TY  - JOUR
AU  - Radisavljević, Anđela
AU  - Stojanović, Dušica B.
AU  - Petrović, Miloš
AU  - Radojević, Vesna
AU  - Uskoković, Petar
AU  - Rajilić-Stojanović, Mirjana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5295
AB  - In this study, polycaprolactone (PCL), as a biocompatible polymer was functionalized by addition of medicinal plant extract- Achillea millefolium L. (yarrow). Nanofiber mats were fabricated from PCL solutions containing dry yarrow extract in four concentrations (5%, 10%, 15%, and 20% relative to the weight of the polymer) by using blend electrospinning method. The nanofibers were characterized for their biological, mechanical and drug release behavior. In vitro release of yarrow polyphenols from the electrospun PCL nanofibers over a period of 5 days showed the release of up to 98% of the total loaded polyphenols. The released polyphenols retained its antioxidant activity, which was determined by DPPH assay. Electrospun PCL/yarrow nanofiber mats exhibited the antibacterial effect against Staphylococcus aureus, but had no effect on the growth of Pseudomonas aeruginosa. All PCL/yarrow nanofiber mats had improved mechanical properties compared to the neat PCL nanofibers, as evident by an increase in Young's modulus of elasticity (up to 5.7 times), the tensile strength (up to 5.5 times), and the strain at break (up to 1.45 times). Based on our results, yarrow-loaded PCL nanofiber mats appeared to be multi-functional biomaterials suitable for the production of catheter-coating materials, patches, or gauzes with antibacterial and antioxidant properties.
PB  - John Wiley and Sons Inc.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Electrospun polycaprolactone nanofibers functionalized with Achillea millefolium extract yield biomaterial with antibacterial, antioxidant and improved mechanical properties
EP  - 974
IS  - 7
SP  - 962
VL  - 111
DO  - 10.1002/jbm.a.37481
ER  - 

@article{
author = "Radisavljević, Anđela and Stojanović, Dušica B. and Petrović, Miloš and Radojević, Vesna and Uskoković, Petar and Rajilić-Stojanović, Mirjana",
year = "2023",
abstract = "In this study, polycaprolactone (PCL), as a biocompatible polymer was functionalized by addition of medicinal plant extract- Achillea millefolium L. (yarrow). Nanofiber mats were fabricated from PCL solutions containing dry yarrow extract in four concentrations (5%, 10%, 15%, and 20% relative to the weight of the polymer) by using blend electrospinning method. The nanofibers were characterized for their biological, mechanical and drug release behavior. In vitro release of yarrow polyphenols from the electrospun PCL nanofibers over a period of 5 days showed the release of up to 98% of the total loaded polyphenols. The released polyphenols retained its antioxidant activity, which was determined by DPPH assay. Electrospun PCL/yarrow nanofiber mats exhibited the antibacterial effect against Staphylococcus aureus, but had no effect on the growth of Pseudomonas aeruginosa. All PCL/yarrow nanofiber mats had improved mechanical properties compared to the neat PCL nanofibers, as evident by an increase in Young's modulus of elasticity (up to 5.7 times), the tensile strength (up to 5.5 times), and the strain at break (up to 1.45 times). Based on our results, yarrow-loaded PCL nanofiber mats appeared to be multi-functional biomaterials suitable for the production of catheter-coating materials, patches, or gauzes with antibacterial and antioxidant properties.",
publisher = "John Wiley and Sons Inc.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Electrospun polycaprolactone nanofibers functionalized with Achillea millefolium extract yield biomaterial with antibacterial, antioxidant and improved mechanical properties",
pages = "974-962",
number = "7",
volume = "111",
doi = "10.1002/jbm.a.37481"
}

Radisavljević, A., Stojanović, D. B., Petrović, M., Radojević, V., Uskoković, P.,& Rajilić-Stojanović, M.. (2023). Electrospun polycaprolactone nanofibers functionalized with Achillea millefolium extract yield biomaterial with antibacterial, antioxidant and improved mechanical properties. in Journal of Biomedical Materials Research - Part A
John Wiley and Sons Inc.., 111(7), 962-974.
https://doi.org/10.1002/jbm.a.37481

Radisavljević A, Stojanović DB, Petrović M, Radojević V, Uskoković P, Rajilić-Stojanović M. Electrospun polycaprolactone nanofibers functionalized with Achillea millefolium extract yield biomaterial with antibacterial, antioxidant and improved mechanical properties. in Journal of Biomedical Materials Research - Part A. 2023;111(7):962-974.
doi:10.1002/jbm.a.37481 .

Radisavljević, Anđela, Stojanović, Dušica B., Petrović, Miloš, Radojević, Vesna, Uskoković, Petar, Rajilić-Stojanović, Mirjana, "Electrospun polycaprolactone nanofibers functionalized with Achillea millefolium extract yield biomaterial with antibacterial, antioxidant and improved mechanical properties" in Journal of Biomedical Materials Research - Part A, 111, no. 7 (2023):962-974,
https://doi.org/10.1002/jbm.a.37481 . .

TY  - JOUR
AU  - Bačić, Ana
AU  - Milivojević, Vladimir
AU  - Petković, Isidora
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Medić Brkić, Branislava
AU  - Popadić, Dušan
AU  - Milosavljević, Tomica
AU  - Rajilić-Stojanović, Mirjana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6054
AB  - Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.
T2  - Journal of Fungi
T1  - In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species
IS  - 3
SP  - 328
VL  - 9
DO  - 10.3390/jof9030328
ER  - 

@article{
author = "Bačić, Ana and Milivojević, Vladimir and Petković, Isidora and Kekić, Dušan and Gajić, Ina and Medić Brkić, Branislava and Popadić, Dušan and Milosavljević, Tomica and Rajilić-Stojanović, Mirjana",
year = "2023",
abstract = "Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.",
journal = "Journal of Fungi",
title = "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species",
number = "3",
pages = "328",
volume = "9",
doi = "10.3390/jof9030328"
}

Bačić, A., Milivojević, V., Petković, I., Kekić, D., Gajić, I., Medić Brkić, B., Popadić, D., Milosavljević, T.,& Rajilić-Stojanović, M.. (2023). In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi, 9(3), 328.
https://doi.org/10.3390/jof9030328

Bačić A, Milivojević V, Petković I, Kekić D, Gajić I, Medić Brkić B, Popadić D, Milosavljević T, Rajilić-Stojanović M. In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi. 2023;9(3):328.
doi:10.3390/jof9030328 .

Bačić, Ana, Milivojević, Vladimir, Petković, Isidora, Kekić, Dušan, Gajić, Ina, Medić Brkić, Branislava, Popadić, Dušan, Milosavljević, Tomica, Rajilić-Stojanović, Mirjana, "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species" in Journal of Fungi, 9, no. 3 (2023):328,
https://doi.org/10.3390/jof9030328 . .

TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Božić, Bojan
AU  - Lunić, Tanja
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Nedeljković Božić, Biljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5097
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - MDPI
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis
IS  - 6
SP  - 1273
VL  - 14
DO  - 10.3390/nu14061273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Nedeljković Božić, Biljana",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "MDPI",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis",
number = "6",
pages = "1273",
volume = "14",
doi = "10.3390/nu14061273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Nedeljković Božić, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients
MDPI., 14(6), 1273.
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Nedeljković Božić B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients. 2022;14(6):1273.
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Nedeljković Božić, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis" in Nutrients, 14, no. 6 (2022):1273,
https://doi.org/10.3390/nu14061273 . .

TY  - JOUR
AU  - Ilić, Nevena
AU  - Davidović, Slađana
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Pecarski, Danijela
AU  - Ivancic-Santek, Mirela
AU  - Mihajlovski, Katarina
AU  - Dimitrijević-Branković, Suzana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5001
AB  - This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.
T2  - Biomass Conversion and Biorefinery
T1  - Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production
EP  - 17186
SP  - 17175
VL  - 13
DO  - 10.1007/s13399-021-02145-x
ER  - 

@article{
author = "Ilić, Nevena and Davidović, Slađana and Milić, Marija and Rajilić-Stojanović, Mirjana and Pecarski, Danijela and Ivancic-Santek, Mirela and Mihajlovski, Katarina and Dimitrijević-Branković, Suzana",
year = "2022",
abstract = "This study aimed at the exploitation of lignocellulosic wastes for the evaluation of the newly isolated white-rot fungal strains enzymatic potential for bioethanol production. The isolates belonging to Basidiomycetes, Fomes fomentarius TMF2, Schizophyllum commune TMF3, and Bjerkandera adusta TMF1, could synthesize extracellular laccase and various hydrolase while growing on lignocellulosic waste materials. More specifically, for the first time, F. fomentarius TMF2 synthesized laccase using sunflower meal as a substrate. This substrate could stimulate B. adusta TMF1 for carboxymethyl cellulase and Avicelase production. The isolate B. adusta TMF1 was able to produce amylase during its growth on brewer's spent grain, which is up to now the best result reported for this activity of any B. adusta strain. Soybean meal was the most potent substrate for stimulating pectinase production by B. adusta TMF1 and S. commune TMF3. While growing on brewer 's spent grain, B. adusta TMF1 and S. commune TMF3 produced high levels of xylanase. Spent coffee residues were for the first time tested as a substrate for hydrolase production by selected fungal species. Also, this is the first attempt where the produced enzymes by isolate B. adusta TMF1 were used for lignocellulose hydrolysis of brewer's spent grain and corn stover for bioethanol production, where under non-optimized conditions 0.94 g/L and 0.86 g/L of bioethanol could be produced, respectively. This study showed that novel white-rot fungal isolates, especially B. adusta TMF1, could grow on unexploited, low-cost lignocellulosic substrates and to produce biotechnological value-added products within environmental and economical accepted processes.",
journal = "Biomass Conversion and Biorefinery",
title = "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production",
pages = "17186-17175",
volume = "13",
doi = "10.1007/s13399-021-02145-x"
}

Ilić, N., Davidović, S., Milić, M., Rajilić-Stojanović, M., Pecarski, D., Ivancic-Santek, M., Mihajlovski, K.,& Dimitrijević-Branković, S.. (2022). Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery, 13, 17175-17186.
https://doi.org/10.1007/s13399-021-02145-x

Ilić N, Davidović S, Milić M, Rajilić-Stojanović M, Pecarski D, Ivancic-Santek M, Mihajlovski K, Dimitrijević-Branković S. Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production. in Biomass Conversion and Biorefinery. 2022;13:17175-17186.
doi:10.1007/s13399-021-02145-x .

Ilić, Nevena, Davidović, Slađana, Milić, Marija, Rajilić-Stojanović, Mirjana, Pecarski, Danijela, Ivancic-Santek, Mirela, Mihajlovski, Katarina, Dimitrijević-Branković, Suzana, "Valorization of lignocellulosic wastes for extracellular enzyme production by novel Basidiomycetes: screening, hydrolysis, and bioethanol production" in Biomass Conversion and Biorefinery, 13 (2022):17175-17186,
https://doi.org/10.1007/s13399-021-02145-x . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Miladinović, Marija
AU  - Gašić, Uroš
AU  - Dimitrijević-Branković, Suzana
AU  - Rajilić‑Stojanović, Mirjana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5137
AB  - Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Biomass Conversion and Biorefinery
T1  - Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction
DO  - 10.1007/s13399-022-02717-5
ER  - 

@article{
author = "Milutinović, Milica and Miladinović, Marija and Gašić, Uroš and Dimitrijević-Branković, Suzana and Rajilić‑Stojanović, Mirjana",
year = "2022",
abstract = "Microwave-assisted extraction (MAE) conditions were optimized to improve extract quality of medicinal herb — Hypericum perforatum L. (St. John’s wort) dust. Response surface methodology was applied initially to obtain the highest concentration of total polyphenols in extract solids (MAE-e). St. John’s wort was mixed with 30% ethanol in 50 mL/g solvent to solid ratio, and treated with 170 W microwave power for 40 s to yield an extract with 411.26 ± 6.21 mg GAE/g of polyphenols. This extract contained a significantly higher content of polyphenols (42.50%) and had significantly higher antioxidant activity than the macerate obtained by using European Medicines Agency (EMA) recommended procedure. The advantage of the EMA procedure was the higher yield of extract per gram of plant material. Therefore, another set of MAE conditions was defined to maximize the yield of polyphenols per gram of plant material (MAE-p). The MAE-p extract was produced by using 30% ethanol, 10 mL/g solvent to solid ratio, and 170 W microwave power for 100 s, which was, again, a markedly shorter period than 72 h of maceration. The MAE-p extract had a slightly, but significantly higher yield (5.2%), more polyphenols (8.8%), and improved antioxidant activity compared to the EMA macerate. Antimicrobial activity against several pathogens was stronger for the MAE extracts. The chemical composition of extracts was slightly different and MAE favored extraction of glycosides, in particular, rutin (quercetin-3-O-rutinoside), while the EMA macerate contained quercetin aglycon in the highest concentration. Our study demonstrates that statistically planned experiments allow for significant improvement of the extraction process, which application could facilitate better use of natural resources and deliver more potent extracts than those obtained by currently recommended procedures.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Biomass Conversion and Biorefinery",
title = "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction",
doi = "10.1007/s13399-022-02717-5"
}

Milutinović, M., Miladinović, M., Gašić, U., Dimitrijević-Branković, S.,& Rajilić‑Stojanović, M.. (2022). Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s13399-022-02717-5

Milutinović M, Miladinović M, Gašić U, Dimitrijević-Branković S, Rajilić‑Stojanović M. Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction. in Biomass Conversion and Biorefinery. 2022;.
doi:10.1007/s13399-022-02717-5 .

Milutinović, Milica, Miladinović, Marija, Gašić, Uroš, Dimitrijević-Branković, Suzana, Rajilić‑Stojanović, Mirjana, "Recovery of bioactive molecules from Hypericum perforatum L. dust using microwave-assisted extraction" in Biomass Conversion and Biorefinery (2022),
https://doi.org/10.1007/s13399-022-02717-5 . .

TY  - CHAP
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6125
AB  - Over the course of human evolutionary path, our microbiota has co-evolved to achieve a solid relationship with the human host in various body sites. The most densely populated site is the distal gut and gut microbiota is responsible for many utterly important functions that differ throughout life to meet physiological needs at different ages. A trajectory of microbial development tightly follows that of the human host, with initial unstable and rapidly changing microbial communities that are gradually transformed into a stable microbial ecosystem. During the first years of life, environmental and intrinsic factors affect the microbial assemblage, which when unfavorable may induce microbial dysbiosis and consequently alter the immune system maturation and neurodevelopment. Although adulthood is characterized by long-term stability of the gut microbiota with greater resilience to stressors, profound detrimental factors may lead to alterations in microbial communities. Recently elucidated bidirectional connections between the gut microbiota and distal organs, highlight the association between dysbiosis and the onset of various non-communicable diseases. With emerging evidence showing the importance of diverse and rich microbial communities in maintaining homeostasis and health, gut microbiota appears as a novel highly potent therapeutic target to alleviate ongoing pathophysiological processes and further promote longevity. In this article the characteristics of gut microbiota across human lifespan are discussed, with additional reference to microbiota of ancient and rural societies, with an intention to gain a broader picture and to provide insight into the factors influencing microbial assemblage.
PB  - Elsevier
T2  - Comprehensive Gut Microbiota
T1  - Microbiota changes throughout life - an overview
EP  - 12
SP  - 1
VL  - 2
DO  - 10.1016/B978-0-12-819265-8.00107-8
ER  - 

@inbook{
author = "Bačić, Ana and Rajilić-Stojanović, Mirjana",
year = "2022",
abstract = "Over the course of human evolutionary path, our microbiota has co-evolved to achieve a solid relationship with the human host in various body sites. The most densely populated site is the distal gut and gut microbiota is responsible for many utterly important functions that differ throughout life to meet physiological needs at different ages. A trajectory of microbial development tightly follows that of the human host, with initial unstable and rapidly changing microbial communities that are gradually transformed into a stable microbial ecosystem. During the first years of life, environmental and intrinsic factors affect the microbial assemblage, which when unfavorable may induce microbial dysbiosis and consequently alter the immune system maturation and neurodevelopment. Although adulthood is characterized by long-term stability of the gut microbiota with greater resilience to stressors, profound detrimental factors may lead to alterations in microbial communities. Recently elucidated bidirectional connections between the gut microbiota and distal organs, highlight the association between dysbiosis and the onset of various non-communicable diseases. With emerging evidence showing the importance of diverse and rich microbial communities in maintaining homeostasis and health, gut microbiota appears as a novel highly potent therapeutic target to alleviate ongoing pathophysiological processes and further promote longevity. In this article the characteristics of gut microbiota across human lifespan are discussed, with additional reference to microbiota of ancient and rural societies, with an intention to gain a broader picture and to provide insight into the factors influencing microbial assemblage.",
publisher = "Elsevier",
journal = "Comprehensive Gut Microbiota",
booktitle = "Microbiota changes throughout life - an overview",
pages = "12-1",
volume = "2",
doi = "10.1016/B978-0-12-819265-8.00107-8"
}

Bačić, A.,& Rajilić-Stojanović, M.. (2022). Microbiota changes throughout life - an overview. in Comprehensive Gut Microbiota
Elsevier., 2, 1-12.
https://doi.org/10.1016/B978-0-12-819265-8.00107-8

Bačić A, Rajilić-Stojanović M. Microbiota changes throughout life - an overview. in Comprehensive Gut Microbiota. 2022;2:1-12.
doi:10.1016/B978-0-12-819265-8.00107-8 .

Bačić, Ana, Rajilić-Stojanović, Mirjana, "Microbiota changes throughout life - an overview" in Comprehensive Gut Microbiota, 2 (2022):1-12,
https://doi.org/10.1016/B978-0-12-819265-8.00107-8 . .

TY  - JOUR
AU  - Malfertheiner, Peter
AU  - Megraud, Francis
AU  - Rokkas, Theodore
AU  - Gisbert, Javier P.
AU  - Liou, Jyh-Ming
AU  - Schulz, Christian
AU  - Gasbarrini, Antonio
AU  - Hunt, Richard H.
AU  - Leja, Marcis
AU  - O’Morain, Colm
AU  - Rugge, Massimo
AU  - Suerbaum, Sebastian
AU  - Tilg, Herbert
AU  - Sugano, Kentaro
AU  - El-Omar, Emad M.
AU  - Agreus, L.
AU  - Bazzoli, F
AU  - Bordin, D
AU  - Loginov, A.S.
AU  - Mario, F. D.
AU  - Dinis-Ribeiro, M.
AU  - Engstrand, Lars
AU  - Fallone, C
AU  - Goh, K.L.
AU  - Graham, David
AU  - Kuipers, E.J.
AU  - Kupcinskas, J.
AU  - Lanas, A.
AU  - Machado, Jose C.
AU  - Mahachai, V.
AU  - Marshall, B.J.
AU  - Milosavljevic, Tomica
AU  - Moss, S.F.
AU  - Park, J.Y.
AU  - Niv, Y.
AU  - Rajilić-Stojanović, Mirjana
AU  - Ristimaki, A
AU  - Smith, S.
AU  - Tepeš, Bojan
AU  - Wu, C.Y.
AU  - Zhou, L.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6050
AB  - elicobacter pylori Infection is formally recognised as an infectious disease, an entity that is now included in the International Classification of Diseases 11th Revision. This in principle leads to the recommendation that all infected patients should receive treatment. In the context of the wide clinical spectrum associated with Helicobacter pylori gastritis, specific issues persist and require regular updates for optimised management. The identification of distinct clinical scenarios, proper testing and adoption of effective strategies for prevention of gastric cancer and other complications are addressed. H. pylori treatment is challenged by the continuously rising antibiotic resistance and demands for susceptibility testing with consideration of novel molecular technologies and careful selection of first line and rescue therapies. The role of H. pylori and antibiotic therapies and their impact on the gut microbiota are also considered. Progress made in the management of H. pylori infection is covered in the present sixth edition of the Maastricht/ Florence 2021 Consensus Report, key aspects related to the clinical role of H. pylori infection were re-evaluated and updated. Forty-one experts from 29 countries representing a global community, examined the new data related to H. pylori infection in five working groups: (1) indications/associations, (2) diagnosis, (3) treatment, (4) prevention/gastric cancer and (5) H. pylori and the gut microbiota. The results of the individual working groups were presented for a final consensus voting that included all participants. Recommendations are provided on the basis of the best available evidence and relevance to the management of H. pylori infection in various clinical fields.
T2  - Guidelines
T1  - Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report
EP  - 1762
IS  - 9
SP  - 1724
VL  - 71
DO  - 10.1136/gutjnl-2022-327745
ER  - 

@article{
author = "Malfertheiner, Peter and Megraud, Francis and Rokkas, Theodore and Gisbert, Javier P. and Liou, Jyh-Ming and Schulz, Christian and Gasbarrini, Antonio and Hunt, Richard H. and Leja, Marcis and O’Morain, Colm and Rugge, Massimo and Suerbaum, Sebastian and Tilg, Herbert and Sugano, Kentaro and El-Omar, Emad M. and Agreus, L. and Bazzoli, F and Bordin, D and Loginov, A.S. and Mario, F. D. and Dinis-Ribeiro, M. and Engstrand, Lars and Fallone, C and Goh, K.L. and Graham, David and Kuipers, E.J. and Kupcinskas, J. and Lanas, A. and Machado, Jose C. and Mahachai, V. and Marshall, B.J. and Milosavljevic, Tomica and Moss, S.F. and Park, J.Y. and Niv, Y. and Rajilić-Stojanović, Mirjana and Ristimaki, A and Smith, S. and Tepeš, Bojan and Wu, C.Y. and Zhou, L.",
year = "2022",
abstract = "elicobacter pylori Infection is formally recognised as an infectious disease, an entity that is now included in the International Classification of Diseases 11th Revision. This in principle leads to the recommendation that all infected patients should receive treatment. In the context of the wide clinical spectrum associated with Helicobacter pylori gastritis, specific issues persist and require regular updates for optimised management. The identification of distinct clinical scenarios, proper testing and adoption of effective strategies for prevention of gastric cancer and other complications are addressed. H. pylori treatment is challenged by the continuously rising antibiotic resistance and demands for susceptibility testing with consideration of novel molecular technologies and careful selection of first line and rescue therapies. The role of H. pylori and antibiotic therapies and their impact on the gut microbiota are also considered. Progress made in the management of H. pylori infection is covered in the present sixth edition of the Maastricht/ Florence 2021 Consensus Report, key aspects related to the clinical role of H. pylori infection were re-evaluated and updated. Forty-one experts from 29 countries representing a global community, examined the new data related to H. pylori infection in five working groups: (1) indications/associations, (2) diagnosis, (3) treatment, (4) prevention/gastric cancer and (5) H. pylori and the gut microbiota. The results of the individual working groups were presented for a final consensus voting that included all participants. Recommendations are provided on the basis of the best available evidence and relevance to the management of H. pylori infection in various clinical fields.",
journal = "Guidelines",
title = "Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report",
pages = "1762-1724",
number = "9",
volume = "71",
doi = "10.1136/gutjnl-2022-327745"
}

Malfertheiner, P., Megraud, F., Rokkas, T., Gisbert, J. P., Liou, J., Schulz, C., Gasbarrini, A., Hunt, R. H., Leja, M., O’Morain, C., Rugge, M., Suerbaum, S., Tilg, H., Sugano, K., El-Omar, E. M., Agreus, L., Bazzoli, F., Bordin, D., Loginov, A.S., Mario, F. D., Dinis-Ribeiro, M., Engstrand, L., Fallone, C., Goh, K.L., Graham, D., Kuipers, E.J., Kupcinskas, J., Lanas, A., Machado, J. C., Mahachai, V., Marshall, B.J., Milosavljevic, T., Moss, S.F., Park, J.Y., Niv, Y., Rajilić-Stojanović, M., Ristimaki, A., Smith, S., Tepeš, B., Wu, C.Y.,& Zhou, L.. (2022). Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report. in Guidelines, 71(9), 1724-1762.
https://doi.org/10.1136/gutjnl-2022-327745

Malfertheiner P, Megraud F, Rokkas T, Gisbert JP, Liou J, Schulz C, Gasbarrini A, Hunt RH, Leja M, O’Morain C, Rugge M, Suerbaum S, Tilg H, Sugano K, El-Omar EM, Agreus L, Bazzoli F, Bordin D, Loginov A, Mario FD, Dinis-Ribeiro M, Engstrand L, Fallone C, Goh K, Graham D, Kuipers E, Kupcinskas J, Lanas A, Machado JC, Mahachai V, Marshall B, Milosavljevic T, Moss S, Park J, Niv Y, Rajilić-Stojanović M, Ristimaki A, Smith S, Tepeš B, Wu C, Zhou L. Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report. in Guidelines. 2022;71(9):1724-1762.
doi:10.1136/gutjnl-2022-327745 .

Malfertheiner, Peter, Megraud, Francis, Rokkas, Theodore, Gisbert, Javier P., Liou, Jyh-Ming, Schulz, Christian, Gasbarrini, Antonio, Hunt, Richard H., Leja, Marcis, O’Morain, Colm, Rugge, Massimo, Suerbaum, Sebastian, Tilg, Herbert, Sugano, Kentaro, El-Omar, Emad M., Agreus, L., Bazzoli, F, Bordin, D, Loginov, A.S., Mario, F. D., Dinis-Ribeiro, M., Engstrand, Lars, Fallone, C, Goh, K.L., Graham, David, Kuipers, E.J., Kupcinskas, J., Lanas, A., Machado, Jose C., Mahachai, V., Marshall, B.J., Milosavljevic, Tomica, Moss, S.F., Park, J.Y., Niv, Y., Rajilić-Stojanović, Mirjana, Ristimaki, A, Smith, S., Tepeš, Bojan, Wu, C.Y., Zhou, L., "Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report" in Guidelines, 71, no. 9 (2022):1724-1762,
https://doi.org/10.1136/gutjnl-2022-327745 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/20
AB  - Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .
PB  - Springer, Dordrecht
T2  - Waste and Biomass Valorization
T1  - From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production
EP  - 174
IS  - 1
SP  - 165
VL  - 12
DO  - 10.1007/s12649-020-00960-3
ER  - 

@article{
author = "Mihajlovski, Katarina and Buntić, Aneta and Milić, Marija and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Purpose To avoid a negative environmental and economic impact of agricultural wastes, and following the principles of circular economy, the reuse of agricultural wastes is necessary. For this purpose, isolation of novel microorganisms with potential biotechnological application is recommended. The current researches in bioethanol production are aimed to reduce the production costs using low-cost substrates and in-house produced enzymes by novel isolated microorganisms. In line with this, in this study valorization of these agricultural by-products by novel isolate S. fulvissimus CKS7 to biotechnological value added products was done. Methods Standard microbiological methods were used for the isolation and characterization of strain. Enzymes activities were determinated using DNS method while, the ethanol concentration was determined based on the density of the alcohol distillate at 20 degrees C. Results The maximal enzymatic activities for amylase, cellulases (carboxymethyl cellulase and Avicelase), pectinase and xylanase were achieved using rye bran as a waste substrate for CKS7 growth. Obtained crude bacterial enzymes were used for enzymatic hydrolysis of lignocellulosic materials including horsetail waste, yellow gentian waste, corn stover, cotton material and corona pre-treated cotton material. The maximum yield of reducing sugars was obtained on horsetail waste and corona pre-treated cotton material. Waste brewer's yeast Saccharomyces cerevisiae was successfully used for the production of bioethanol using horsetail waste hydrolysate and corona pre-treated cotton material hydrolysate. Conclusion The obtained results showed that bacterial strain CKS7 has a significant, still unexplored enzymatic potential that could be used to achieve a cleaner, environmental friendly and economically acceptable biofuel production. [GRAPHICS] .",
publisher = "Springer, Dordrecht",
journal = "Waste and Biomass Valorization",
title = "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production",
pages = "174-165",
number = "1",
volume = "12",
doi = "10.1007/s12649-020-00960-3"
}

Mihajlovski, K., Buntić, A., Milić, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization
Springer, Dordrecht., 12(1), 165-174.
https://doi.org/10.1007/s12649-020-00960-3

Mihajlovski K, Buntić A, Milić M, Rajilić-Stojanović M, Dimitrijević-Branković S. From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production. in Waste and Biomass Valorization. 2021;12(1):165-174.
doi:10.1007/s12649-020-00960-3 .

Mihajlovski, Katarina, Buntić, Aneta, Milić, Marija, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "From Agricultural Waste to Biofuel: Enzymatic Potential of a Bacterial Isolate Streptomyces fulvissimus CKS7 for Bioethanol Production" in Waste and Biomass Valorization, 12, no. 1 (2021):165-174,
https://doi.org/10.1007/s12649-020-00960-3 . .

TY  - JOUR
AU  - Miljković, Miona
AU  - Davidović, Slađana
AU  - Đukić-Vuković, Aleksandra
AU  - Ilic, Mila, V
AU  - Simović, Milica
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4791
AB  - Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.
T2  - Hemijska industrija
T1  - Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology
EP  - 146
IS  - 3
SP  - 135
VL  - 75
DO  - 10.2298/HEMIND200710015M
ER  - 

@article{
author = "Miljković, Miona and Davidović, Slađana and Đukić-Vuković, Aleksandra and Ilic, Mila, V and Simović, Milica and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "Dextransucrase (DS) is a glucosyltransferase (E. C. 2.4.1.5) that catalyzes the transfer of glucosyl residues from sucrose to dextran polymer and liberates fructose. This enzyme isassociated with a wide application range of dextran and oligosaccharides. DS production by Leuconostoc mesenteroidesT3 was optimized using a Central Composite Design under the Response Surface Methodology. Three variables were chosen for optimization: distillery stillage, sucrose and manganese concentration. The results showed that sucrose and manganese concentrations had a positive linear effect on DS production while all variable interactions (stillage-manganese, stillage-sucrose, and sucrose-manganese) had significant influences on the DS production. The maximal DS yield of 3.391 +/- 0.131 U cm-3, was obtained in the medium with 64.33 % distillery stillage concentration, 5.30% sucrose concentration and 0.022 % manganese concentration. Our study revealed the potential of distillery stillage combined with sugar beet molasses, supplemented with sucrose and manganese to be employed as a valuable medium growth for lactic acid bacteria and production of DS. Also, taking into consideration the origin of the substrates, utilization of industrial by-products in this way has a great environmental relevance and is in accordance with circular economy.",
journal = "Hemijska industrija",
title = "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology",
pages = "146-135",
number = "3",
volume = "75",
doi = "10.2298/HEMIND200710015M"
}

Miljković, M., Davidović, S., Đukić-Vuković, A., Ilic, M. V., Simović, M., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija, 75(3), 135-146.
https://doi.org/10.2298/HEMIND200710015M

Miljković M, Davidović S, Đukić-Vuković A, Ilic MV, Simović M, Rajilić-Stojanović M, Dimitrijević-Branković S. Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology. in Hemijska industrija. 2021;75(3):135-146.
doi:10.2298/HEMIND200710015M .

Miljković, Miona, Davidović, Slađana, Đukić-Vuković, Aleksandra, Ilic, Mila, V, Simović, Milica, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Utilization of agro-industrial by-products as substrates for dextransucrase production by Leuconostoc mesenteroides T3: process optimization using response surface methodology" in Hemijska industrija, 75, no. 3 (2021):135-146,
https://doi.org/10.2298/HEMIND200710015M . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Pecarski, Danijela
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4827
AB  - During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.
T2  - Environmental Technology & Innovation
T1  - Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3
VL  - 23
DO  - 10.1016/j.eti.2021.101627
ER  - 

@article{
author = "Mihajlovski, Katarina and Pecarski, Danijela and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2021",
abstract = "During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.",
journal = "Environmental Technology & Innovation",
title = "Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3",
volume = "23",
doi = "10.1016/j.eti.2021.101627"
}

Mihajlovski, K., Pecarski, D., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3. in Environmental Technology & Innovation, 23.
https://doi.org/10.1016/j.eti.2021.101627

Mihajlovski K, Pecarski D, Rajilić-Stojanović M, Dimitrijević-Branković S. Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3. in Environmental Technology & Innovation. 2021;23.
doi:10.1016/j.eti.2021.101627 .

Mihajlovski, Katarina, Pecarski, Danijela, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3" in Environmental Technology & Innovation, 23 (2021),
https://doi.org/10.1016/j.eti.2021.101627 . .

TY  - JOUR
AU  - Milutinović, Milica
AU  - Dimitrijević-Branković, Suzana
AU  - Rajilić-Stojanović, Mirjana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4858
AB  - Medicinal plants and their extracts contain substantial quantities of polyphenols. As metabolically active plant metabolites, polyphenols are food components with a wide range of biological activities. Given their poor absorbability in the digestive tract their activity toward the human host is typically mediated through interaction with intestinal microbes. As a result, polyphenols comprise a novel group of prebiotics. In this study, we tested the effect of five polyphenol-rich extracts from four medicinal herbs on the growth of probiotic and pathogenic microbes. The studied medicinal herbs were Gentiana asclepiadea L. (willow gentian), Hypericum perforatum L. (St. John's wort), Satureja montana L. (winter savory), and Achillea millefolium L. (yarrow). All these plants are traditionally used for the treatment of digestive problems. Extracts were prepared using safe solvent combinations. We tested the impact of addition of plant extracts on the growth of three probiotic lactobacilli and probiotic yeast Saccharomyces boulardii. The effect of addition of plant extracts to liquid media (concentration range 0.25-10 mg/mL) on the growth of probiotics, was tested in vitro. The antimicrobial activity of the extracts was tested against several opportunistic bacteria and yeast. St. John's wort, winter savory, and willow gentian extracts showed a stimulative effect on probiotic yeast growth, while the highest growth-stimulating effect was achieved when microwave-assisted yarrow extract was used in the concentration of 0.5 mg/mL. Under these conditions growth of S. boulardii was increased 130-fold. In addition, the yarrow extract stimulated the growth of Lactiplantibacillus plantarum 299v. The growth of two Lacticasibacillus rhamnosus strains was not stimulated by the addition of any extracts. Our results show that plant polyphenol-rich extracts can influence the growth of microorganisms that are typical members of the intestinal microbiota. For the first time we demonstrate that probiotic yeast growth can be stimulated by extracts of medicinal herbs, which when accompanied by suppression of Candida yeasts suggests a potential benefit of the treatment in diseases that are associated with fungal dysbiosis.
T2  - Frontiers in Nutrition
T1  - Plant Extracts Rich in Polyphenols as Potent Modulators in the Growth of Probiotic and Pathogenic Intestinal Microorganisms
VL  - 8
DO  - 10.3389/fnut.2021.688843
ER  - 

@article{
author = "Milutinović, Milica and Dimitrijević-Branković, Suzana and Rajilić-Stojanović, Mirjana",
year = "2021",
abstract = "Medicinal plants and their extracts contain substantial quantities of polyphenols. As metabolically active plant metabolites, polyphenols are food components with a wide range of biological activities. Given their poor absorbability in the digestive tract their activity toward the human host is typically mediated through interaction with intestinal microbes. As a result, polyphenols comprise a novel group of prebiotics. In this study, we tested the effect of five polyphenol-rich extracts from four medicinal herbs on the growth of probiotic and pathogenic microbes. The studied medicinal herbs were Gentiana asclepiadea L. (willow gentian), Hypericum perforatum L. (St. John's wort), Satureja montana L. (winter savory), and Achillea millefolium L. (yarrow). All these plants are traditionally used for the treatment of digestive problems. Extracts were prepared using safe solvent combinations. We tested the impact of addition of plant extracts on the growth of three probiotic lactobacilli and probiotic yeast Saccharomyces boulardii. The effect of addition of plant extracts to liquid media (concentration range 0.25-10 mg/mL) on the growth of probiotics, was tested in vitro. The antimicrobial activity of the extracts was tested against several opportunistic bacteria and yeast. St. John's wort, winter savory, and willow gentian extracts showed a stimulative effect on probiotic yeast growth, while the highest growth-stimulating effect was achieved when microwave-assisted yarrow extract was used in the concentration of 0.5 mg/mL. Under these conditions growth of S. boulardii was increased 130-fold. In addition, the yarrow extract stimulated the growth of Lactiplantibacillus plantarum 299v. The growth of two Lacticasibacillus rhamnosus strains was not stimulated by the addition of any extracts. Our results show that plant polyphenol-rich extracts can influence the growth of microorganisms that are typical members of the intestinal microbiota. For the first time we demonstrate that probiotic yeast growth can be stimulated by extracts of medicinal herbs, which when accompanied by suppression of Candida yeasts suggests a potential benefit of the treatment in diseases that are associated with fungal dysbiosis.",
journal = "Frontiers in Nutrition",
title = "Plant Extracts Rich in Polyphenols as Potent Modulators in the Growth of Probiotic and Pathogenic Intestinal Microorganisms",
volume = "8",
doi = "10.3389/fnut.2021.688843"
}

Milutinović, M., Dimitrijević-Branković, S.,& Rajilić-Stojanović, M.. (2021). Plant Extracts Rich in Polyphenols as Potent Modulators in the Growth of Probiotic and Pathogenic Intestinal Microorganisms. in Frontiers in Nutrition, 8.
https://doi.org/10.3389/fnut.2021.688843

Milutinović M, Dimitrijević-Branković S, Rajilić-Stojanović M. Plant Extracts Rich in Polyphenols as Potent Modulators in the Growth of Probiotic and Pathogenic Intestinal Microorganisms. in Frontiers in Nutrition. 2021;8.
doi:10.3389/fnut.2021.688843 .

Milutinović, Milica, Dimitrijević-Branković, Suzana, Rajilić-Stojanović, Mirjana, "Plant Extracts Rich in Polyphenols as Potent Modulators in the Growth of Probiotic and Pathogenic Intestinal Microorganisms" in Frontiers in Nutrition, 8 (2021),
https://doi.org/10.3389/fnut.2021.688843 . .

TY  - JOUR
AU  - Radisavljević, Anđela
AU  - Milutinović, Milica
AU  - Dimitrijević-Branković, Suzana
AU  - Uskoković, Petar
AU  - Rajilić-Stojanović, Mirjana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4948
AB  - In this study, a spray drying process of yarrow (Achillea millefolium L.) liquid extracts was optimized by using the response surface methodology. The study aimed to determine the influence of temperature (120-195 degrees C), liquid flow rate (3-16.5 cm(3) min(-1)) and dry matter content in the liquid extract (0.3-1.5 %) on the yield of the drying process, the total polyphenols content and the antioxidant activity of the dry powder. Under the tested conditions the yield varied dramatically and ranged from 8 to 75 %, while the effects on the polyphenol content and antioxidant activity were lower. The optimized conditions for the maximum antioxidant activity and maximal yield of the dried extract were as follows: temperature of 130 degrees C, liquid flow rate of 7.5 cm(3) min(-1) and dry matter content of 1.2 %. Under the optimal conditions, the yield was 66 %, while there was a slight decrease in the polyphenol content in the dried extract as compared to that in the liquid extract (145 mg of gallic acid equivalents [GAE] per g of the total dry matter vs. 152 mg GAE g(-1), respectively). Consequently, antioxidant activity of the dry powder was only slightly reduced as compared to that of the liquid extract (DPPH neutralization was 58 vs. 64 %, respectively). The dried yarrow powder preserved its antimicrobial activity against pathogenic bacteria Staphylococcus aureus (MIC value of 10 mg cm(-3)) and Pseudomonas aeruginosa (MIC value of 20 mg cm(-3)).
T2  - Hemijska industrija
T1  - Optimization of spray drying conditions for production of Achillea millefolium extract powder
EP  - 363
IS  - 6
SP  - 353
VL  - 75
DO  - 10.2298/HEMIND210527030R
ER  - 

@article{
author = "Radisavljević, Anđela and Milutinović, Milica and Dimitrijević-Branković, Suzana and Uskoković, Petar and Rajilić-Stojanović, Mirjana",
year = "2021",
abstract = "In this study, a spray drying process of yarrow (Achillea millefolium L.) liquid extracts was optimized by using the response surface methodology. The study aimed to determine the influence of temperature (120-195 degrees C), liquid flow rate (3-16.5 cm(3) min(-1)) and dry matter content in the liquid extract (0.3-1.5 %) on the yield of the drying process, the total polyphenols content and the antioxidant activity of the dry powder. Under the tested conditions the yield varied dramatically and ranged from 8 to 75 %, while the effects on the polyphenol content and antioxidant activity were lower. The optimized conditions for the maximum antioxidant activity and maximal yield of the dried extract were as follows: temperature of 130 degrees C, liquid flow rate of 7.5 cm(3) min(-1) and dry matter content of 1.2 %. Under the optimal conditions, the yield was 66 %, while there was a slight decrease in the polyphenol content in the dried extract as compared to that in the liquid extract (145 mg of gallic acid equivalents [GAE] per g of the total dry matter vs. 152 mg GAE g(-1), respectively). Consequently, antioxidant activity of the dry powder was only slightly reduced as compared to that of the liquid extract (DPPH neutralization was 58 vs. 64 %, respectively). The dried yarrow powder preserved its antimicrobial activity against pathogenic bacteria Staphylococcus aureus (MIC value of 10 mg cm(-3)) and Pseudomonas aeruginosa (MIC value of 20 mg cm(-3)).",
journal = "Hemijska industrija",
title = "Optimization of spray drying conditions for production of Achillea millefolium extract powder",
pages = "363-353",
number = "6",
volume = "75",
doi = "10.2298/HEMIND210527030R"
}

Radisavljević, A., Milutinović, M., Dimitrijević-Branković, S., Uskoković, P.,& Rajilić-Stojanović, M.. (2021). Optimization of spray drying conditions for production of Achillea millefolium extract powder. in Hemijska industrija, 75(6), 353-363.
https://doi.org/10.2298/HEMIND210527030R

Radisavljević A, Milutinović M, Dimitrijević-Branković S, Uskoković P, Rajilić-Stojanović M. Optimization of spray drying conditions for production of Achillea millefolium extract powder. in Hemijska industrija. 2021;75(6):353-363.
doi:10.2298/HEMIND210527030R .

Radisavljević, Anđela, Milutinović, Milica, Dimitrijević-Branković, Suzana, Uskoković, Petar, Rajilić-Stojanović, Mirjana, "Optimization of spray drying conditions for production of Achillea millefolium extract powder" in Hemijska industrija, 75, no. 6 (2021):353-363,
https://doi.org/10.2298/HEMIND210527030R . .

TY  - JOUR
AU  - Milovanović, Tamara
AU  - Pantić, Ivana
AU  - Dragašević, Sanja
AU  - Lugonja, Sofija
AU  - Dumić, Igor
AU  - Rajilić-Stojanović, Mirjana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4724
AB  - Non-alcoholic  fatty  liver  disease  and  colonic  diverticulosis  are  widespread,  obesity-related  diseases.  It  has  
recently  become  clear  that  non-alcoholic  fatty  liver  disease  is  a  systemic  disease  and  may  play  a  key  role  
in  metabolic  syndrome;  therefore,  the  term  metabolic-dysfunction-associated  fatty  liver  disease  has  been  
introduced in the literature. Excess visceral adipose tissue is an important predictor of complications in both 
non-alcoholic fatty liver disease and colonic diverticulosis. Current evidence suggests that intestinal dysbiosis 
may be involved in the development of both non-alcoholic fatty liver disease and colonic diverticulosis, and 
that metabolic syndrome is a consequence rather than a cause of this complex relationship. In this review, our 
aim was to assess the current knowledge of the complex interplay between metabolic syndrome, non-alcoholic 
fatty liver disease, and colonic diverticulosis.
PB  - Romanian Society of Gastroenterology
T2  - Journal of Gastrointestinal and Liver Diseases
T1  - The interrelationship among non-alcoholic fatty liver disease, colonic diverticulosis and metabolic syndrome
EP  - 9
IS  - 2
SP  - 1
VL  - 30
DO  - 10.15403/jgld-3308
ER  - 

@article{
author = "Milovanović, Tamara and Pantić, Ivana and Dragašević, Sanja and Lugonja, Sofija and Dumić, Igor and Rajilić-Stojanović, Mirjana",
year = "2021",
abstract = "Non-alcoholic  fatty  liver  disease  and  colonic  diverticulosis  are  widespread,  obesity-related  diseases.  It  has  
recently  become  clear  that  non-alcoholic  fatty  liver  disease  is  a  systemic  disease  and  may  play  a  key  role  
in  metabolic  syndrome;  therefore,  the  term  metabolic-dysfunction-associated  fatty  liver  disease  has  been  
introduced in the literature. Excess visceral adipose tissue is an important predictor of complications in both 
non-alcoholic fatty liver disease and colonic diverticulosis. Current evidence suggests that intestinal dysbiosis 
may be involved in the development of both non-alcoholic fatty liver disease and colonic diverticulosis, and 
that metabolic syndrome is a consequence rather than a cause of this complex relationship. In this review, our 
aim was to assess the current knowledge of the complex interplay between metabolic syndrome, non-alcoholic 
fatty liver disease, and colonic diverticulosis.",
publisher = "Romanian Society of Gastroenterology",
journal = "Journal of Gastrointestinal and Liver Diseases",
title = "The interrelationship among non-alcoholic fatty liver disease, colonic diverticulosis and metabolic syndrome",
pages = "9-1",
number = "2",
volume = "30",
doi = "10.15403/jgld-3308"
}

Milovanović, T., Pantić, I., Dragašević, S., Lugonja, S., Dumić, I.,& Rajilić-Stojanović, M.. (2021). The interrelationship among non-alcoholic fatty liver disease, colonic diverticulosis and metabolic syndrome. in Journal of Gastrointestinal and Liver Diseases
Romanian Society of Gastroenterology., 30(2), 1-9.
https://doi.org/10.15403/jgld-3308

Milovanović T, Pantić I, Dragašević S, Lugonja S, Dumić I, Rajilić-Stojanović M. The interrelationship among non-alcoholic fatty liver disease, colonic diverticulosis and metabolic syndrome. in Journal of Gastrointestinal and Liver Diseases. 2021;30(2):1-9.
doi:10.15403/jgld-3308 .

Milovanović, Tamara, Pantić, Ivana, Dragašević, Sanja, Lugonja, Sofija, Dumić, Igor, Rajilić-Stojanović, Mirjana, "The interrelationship among non-alcoholic fatty liver disease, colonic diverticulosis and metabolic syndrome" in Journal of Gastrointestinal and Liver Diseases, 30, no. 2 (2021):1-9,
https://doi.org/10.15403/jgld-3308 . .

TY  - JOUR
AU  - Vork, Lisa
AU  - Penders, John
AU  - Jalanka, Jonna
AU  - Bojic, Svetlana
AU  - van Kuijk, Sander M. J.
AU  - Salonen, Anne
AU  - de Vos, Willem M.
AU  - Rajilić-Stojanović, Mirjana
AU  - Weerts, Zsa Zsa R. M.
AU  - Masclee, Ad A. M.
AU  - Pozuelo, Marta
AU  - Manichanh, Chaysavanh
AU  - Jonkers, Daisy M. A. E.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4854
AB  - Introduction Stool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days. Methods Twelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined. Results Linear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this. Conclusion This study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual's microbiota at a given time point. NCT00775060.
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?
VL  - 11
DO  - 10.3389/fcimb.2021.639667
ER  - 

@article{
author = "Vork, Lisa and Penders, John and Jalanka, Jonna and Bojic, Svetlana and van Kuijk, Sander M. J. and Salonen, Anne and de Vos, Willem M. and Rajilić-Stojanović, Mirjana and Weerts, Zsa Zsa R. M. and Masclee, Ad A. M. and Pozuelo, Marta and Manichanh, Chaysavanh and Jonkers, Daisy M. A. E.",
year = "2021",
abstract = "Introduction Stool consistency has been associated with fecal microbial composition. Stool consistency often varies over time, in subjects with and without gastrointestinal disorders, raising the question whether variability in the microbial composition should be considered in microbiota studies. We evaluated within-subject day-to-day variability in stool consistency and the association with the fecal microbiota in irritable bowel syndrome (IBS) and healthy subjects, over seven days. Methods Twelve IBS patients and 12 healthy subjects collected fecal samples during seven consecutive days. Stool consistency was determined by the patient-reported Bristol Stool Scale (BSS) and fecal dry weight percentage. 16S rRNA V4 gene sequencing was performed and microbial richness (alpha diversity; Chao1 index, observed number of species, effective Shannon index) and microbial community structure (beta diversity; Bray-Curtis distance, generalized UniFrac, and taxa abundance on family level) were determined. Results Linear mixed-effects models showed significant associations between stool consistency and microbial richness, but no time effect. This implies that between-subject but not within-subject variation in microbiota over time can partially be explained by variation in stool consistency. Redundancy analysis showed a significant association between stool consistency and microbial community structure, but additional linear mixed-effects models did not demonstrate a time effect on this. Conclusion This study supports an association between stool consistency and fecal microbiota, but no effect of day-to-day fluctuations in stool consistency within seven days. This consolidates the importance of considering stool consistency in gut microbiota research, though confirms the validity of single fecal sampling to represent an individual's microbiota at a given time point. NCT00775060.",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?",
volume = "11",
doi = "10.3389/fcimb.2021.639667"
}

Vork, L., Penders, J., Jalanka, J., Bojic, S., van Kuijk, S. M. J., Salonen, A., de Vos, W. M., Rajilić-Stojanović, M., Weerts, Z. Z. R. M., Masclee, A. A. M., Pozuelo, M., Manichanh, C.,& Jonkers, D. M. A. E.. (2021). Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?. in Frontiers in Cellular and Infection Microbiology, 11.
https://doi.org/10.3389/fcimb.2021.639667

Vork L, Penders J, Jalanka J, Bojic S, van Kuijk SMJ, Salonen A, de Vos WM, Rajilić-Stojanović M, Weerts ZZRM, Masclee AAM, Pozuelo M, Manichanh C, Jonkers DMAE. Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?. in Frontiers in Cellular and Infection Microbiology. 2021;11.
doi:10.3389/fcimb.2021.639667 .

Vork, Lisa, Penders, John, Jalanka, Jonna, Bojic, Svetlana, van Kuijk, Sander M. J., Salonen, Anne, de Vos, Willem M., Rajilić-Stojanović, Mirjana, Weerts, Zsa Zsa R. M., Masclee, Ad A. M., Pozuelo, Marta, Manichanh, Chaysavanh, Jonkers, Daisy M. A. E., "Does Day-to-Day Variability in Stool Consistency Link to the Fecal Microbiota Composition?" in Frontiers in Cellular and Infection Microbiology, 11 (2021),
https://doi.org/10.3389/fcimb.2021.639667 . .

TY  - JOUR
AU  - Milovanovic, Tamara
AU  - Pantic, Ivana
AU  - Dragasevic, Sanja
AU  - Lugonja, Sofija
AU  - Dumic, Igor
AU  - Rajilić-Stojanović, Mirjana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4920
AB  - Non-alcoholic fatty liver disease and colonic diverticulosis are widespread, obesity-related diseases. It has recently become clear that non-alcoholic fatty liver disease is a systemic disease and may play a key role in metabolic syndrome; therefore, the term metabolic-dysfunction-associated fatty liver disease has been introduced in the literature. Excess visceral adipose tissue is an important predictor of complications in both non-alcoholic fatty liver disease and colonic diverticulosis. Current evidence suggests that intestinal dysbiosis may be involved in the development of both non-alcoholic fatty liver disease and colonic diverticulosis, and that metabolic syndrome is a consequence rather than a cause of this complex relationship. In this review, our aim was to assess the current knowledge of the complex interplay between metabolic syndrome, non-alcoholic fatty liver disease, and colonic diverticulosis.
T2  - Journal of Gastrointestinal and Liver Diseases
T1  - The Interrelationship Among Non-Alcoholic Fatty Liver Disease, Colonic Diverticulosis and Metabolic Syndrome
EP  - 282
IS  - 2
SP  - 274
VL  - 30
DO  - 10.15403/jgld-3308
ER  - 

@article{
author = "Milovanovic, Tamara and Pantic, Ivana and Dragasevic, Sanja and Lugonja, Sofija and Dumic, Igor and Rajilić-Stojanović, Mirjana",
year = "2021",
abstract = "Non-alcoholic fatty liver disease and colonic diverticulosis are widespread, obesity-related diseases. It has recently become clear that non-alcoholic fatty liver disease is a systemic disease and may play a key role in metabolic syndrome; therefore, the term metabolic-dysfunction-associated fatty liver disease has been introduced in the literature. Excess visceral adipose tissue is an important predictor of complications in both non-alcoholic fatty liver disease and colonic diverticulosis. Current evidence suggests that intestinal dysbiosis may be involved in the development of both non-alcoholic fatty liver disease and colonic diverticulosis, and that metabolic syndrome is a consequence rather than a cause of this complex relationship. In this review, our aim was to assess the current knowledge of the complex interplay between metabolic syndrome, non-alcoholic fatty liver disease, and colonic diverticulosis.",
journal = "Journal of Gastrointestinal and Liver Diseases",
title = "The Interrelationship Among Non-Alcoholic Fatty Liver Disease, Colonic Diverticulosis and Metabolic Syndrome",
pages = "282-274",
number = "2",
volume = "30",
doi = "10.15403/jgld-3308"
}

Milovanovic, T., Pantic, I., Dragasevic, S., Lugonja, S., Dumic, I.,& Rajilić-Stojanović, M.. (2021). The Interrelationship Among Non-Alcoholic Fatty Liver Disease, Colonic Diverticulosis and Metabolic Syndrome. in Journal of Gastrointestinal and Liver Diseases, 30(2), 274-282.
https://doi.org/10.15403/jgld-3308

Milovanovic T, Pantic I, Dragasevic S, Lugonja S, Dumic I, Rajilić-Stojanović M. The Interrelationship Among Non-Alcoholic Fatty Liver Disease, Colonic Diverticulosis and Metabolic Syndrome. in Journal of Gastrointestinal and Liver Diseases. 2021;30(2):274-282.
doi:10.15403/jgld-3308 .

Milovanovic, Tamara, Pantic, Ivana, Dragasevic, Sanja, Lugonja, Sofija, Dumic, Igor, Rajilić-Stojanović, Mirjana, "The Interrelationship Among Non-Alcoholic Fatty Liver Disease, Colonic Diverticulosis and Metabolic Syndrome" in Journal of Gastrointestinal and Liver Diseases, 30, no. 2 (2021):274-282,
https://doi.org/10.15403/jgld-3308 . .

TY  - JOUR
AU  - Mihajlovski, Katarina
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4536
AB  - Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Renewable Energy
T1  - Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production
EP  - 633
SP  - 627
VL  - 152
DO  - 10.1016/j.renene.2020.01.101
ER  - 

@article{
author = "Mihajlovski, Katarina and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2020",
abstract = "Microbial hydrolytic enzymes are relevant biotechnological products that can be applied in various industries. In this study, we have tested the activity of hydrolytic enzymes of a newly isolated Hymenobacter sp. CKS3 strain and showed, for the first time, that members of Hymenobacter genus have still unexplored hydrolytic potential. Crude hydrolytic enzymes, produced by the strain CKS3 on a waste medium, were incorporated into a process of bioethanol production using waste bread. The conditions for bread hydrolysis were optimized using statistical design. Waste bread hydrolysate obtained under optimal conditions (100.73 h of hydrolysis, waste bread concentration 20.36% and agitation speed 200 rpm) contained 19.89 g/l of reducing sugars. A high performance liquid chromatography of hydrolyzed waste bread samples showed that the main components of the hydrolysate were dextrins, maltotriose, maltose and glucose. When using this substrate and waste baker's yeast for ethanol production under non-optimized conditions 1.73% of ethanol was produced. The results of this study showed that a newly isolated Hymenobacter sp. CKS3 can be utilized for enzymatic hydrolysis and bioethanol production in a process relying on waste materials. Furthermore, it was demonstrated that members of Hymenobacter genus have a significant and currently unexplored potential for bio-based industrial applications.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Renewable Energy",
title = "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production",
pages = "633-627",
volume = "152",
doi = "10.1016/j.renene.2020.01.101"
}

Mihajlovski, K., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2020). Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production. in Renewable Energy
Pergamon-Elsevier Science Ltd, Oxford., 152, 627-633.
https://doi.org/10.1016/j.renene.2020.01.101

Mihajlovski K, Rajilić-Stojanović M, Dimitrijević-Branković S. Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production. in Renewable Energy. 2020;152:627-633.
doi:10.1016/j.renene.2020.01.101 .

Mihajlovski, Katarina, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Enzymatic hydrolysis of waste bread by newly isolated Hymenobacter sp. CKS3: Statistical optimization and bioethanol production" in Renewable Energy, 152 (2020):627-633,
https://doi.org/10.1016/j.renene.2020.01.101 . .

TY  - JOUR
AU  - Swann, J. R.
AU  - Rajilić-Stojanović, Mirjana
AU  - Salonen, Anne
AU  - Sakwinska, O.
AU  - Gill, C.
AU  - Meynier, A.
AU  - Fanca-Berthon, P.
AU  - Schelkle, B.
AU  - Segata, N.
AU  - Shortt, C.
AU  - Tuohy, K.
AU  - Hasselwander, O.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4473
AB  - With the growing appreciation for the influence of the intestinal microbiota on human health, there is increasing motivation to design and refine interventions to promote favorable shifts in the microbiota and their interactions with the host. Technological advances have improved our understanding and ability to measure this indigenous population and the impact of such interventions. However, the rapid growth and evolution of the field, as well as the diversity of methods used, parameters measured and populations studied, make it difficult to interpret the significance of the findings and translate their outcomes to the wider population. This can prevent comparisons across studies and hinder the drawing of appropriate conclusions. This review outlines considerations to facilitate the design, implementation and interpretation of human gut microbiota intervention studies relating to foods based upon our current understanding of the intestinal microbiota, its functionality and interactions with the human host. This includes parameters associated with study design, eligibility criteria, statistical considerations, characterization of products and the measurement of compliance. Methodologies and markers to assess compositional and functional changes in the microbiota, following interventions are discussed in addition to approaches to assess changes in microbiota-host interactions and host responses. Last, EU legislative aspects in relation to foods and health claims are presented. While it is appreciated that the field of gastrointestinal microbiology is rapidly evolving, such guidance will assist in the design and interpretation of human gut microbiota interventional studies relating to foods.
PB  - Springer Heidelberg, Heidelberg
T2  - European Journal of Nutrition
T1  - Considerations for the design and conduct of human gut microbiota intervention studies relating to foods
EP  - 3368
IS  - 8
SP  - 3347
VL  - 59
DO  - 10.1007/s00394-020-02232-1
ER  - 

@article{
author = "Swann, J. R. and Rajilić-Stojanović, Mirjana and Salonen, Anne and Sakwinska, O. and Gill, C. and Meynier, A. and Fanca-Berthon, P. and Schelkle, B. and Segata, N. and Shortt, C. and Tuohy, K. and Hasselwander, O.",
year = "2020",
abstract = "With the growing appreciation for the influence of the intestinal microbiota on human health, there is increasing motivation to design and refine interventions to promote favorable shifts in the microbiota and their interactions with the host. Technological advances have improved our understanding and ability to measure this indigenous population and the impact of such interventions. However, the rapid growth and evolution of the field, as well as the diversity of methods used, parameters measured and populations studied, make it difficult to interpret the significance of the findings and translate their outcomes to the wider population. This can prevent comparisons across studies and hinder the drawing of appropriate conclusions. This review outlines considerations to facilitate the design, implementation and interpretation of human gut microbiota intervention studies relating to foods based upon our current understanding of the intestinal microbiota, its functionality and interactions with the human host. This includes parameters associated with study design, eligibility criteria, statistical considerations, characterization of products and the measurement of compliance. Methodologies and markers to assess compositional and functional changes in the microbiota, following interventions are discussed in addition to approaches to assess changes in microbiota-host interactions and host responses. Last, EU legislative aspects in relation to foods and health claims are presented. While it is appreciated that the field of gastrointestinal microbiology is rapidly evolving, such guidance will assist in the design and interpretation of human gut microbiota interventional studies relating to foods.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "European Journal of Nutrition",
title = "Considerations for the design and conduct of human gut microbiota intervention studies relating to foods",
pages = "3368-3347",
number = "8",
volume = "59",
doi = "10.1007/s00394-020-02232-1"
}

Swann, J. R., Rajilić-Stojanović, M., Salonen, A., Sakwinska, O., Gill, C., Meynier, A., Fanca-Berthon, P., Schelkle, B., Segata, N., Shortt, C., Tuohy, K.,& Hasselwander, O.. (2020). Considerations for the design and conduct of human gut microbiota intervention studies relating to foods. in European Journal of Nutrition
Springer Heidelberg, Heidelberg., 59(8), 3347-3368.
https://doi.org/10.1007/s00394-020-02232-1

Swann JR, Rajilić-Stojanović M, Salonen A, Sakwinska O, Gill C, Meynier A, Fanca-Berthon P, Schelkle B, Segata N, Shortt C, Tuohy K, Hasselwander O. Considerations for the design and conduct of human gut microbiota intervention studies relating to foods. in European Journal of Nutrition. 2020;59(8):3347-3368.
doi:10.1007/s00394-020-02232-1 .

Swann, J. R., Rajilić-Stojanović, Mirjana, Salonen, Anne, Sakwinska, O., Gill, C., Meynier, A., Fanca-Berthon, P., Schelkle, B., Segata, N., Shortt, C., Tuohy, K., Hasselwander, O., "Considerations for the design and conduct of human gut microbiota intervention studies relating to foods" in European Journal of Nutrition, 59, no. 8 (2020):3347-3368,
https://doi.org/10.1007/s00394-020-02232-1 . .

TY  - JOUR
AU  - Rajilić-Stojanović, Mirjana
AU  - Figueiredo, Ceu
AU  - Smet, Annemieke
AU  - Hansen, Richard
AU  - Kupcinskas, Juozas
AU  - Rokkas, Theo
AU  - Andersen, Leif
AU  - Machado, Jose C.
AU  - Ianiro, Gianluca
AU  - Gasbarrini, Antonio
AU  - Leja, Marcis
AU  - Gisbert, Javier P.
AU  - Hold, Georgina L.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4478
AB  - Background Helicobacter pylori is the most infamous constituent of the gastric microbiota and its presence is the strongest risk factor for gastric cancer and other gastroduodenal diseases. Although historically the healthy stomach was considered a sterile organ, we now know it is colonised with a complex microbiota. However, its role in health and disease is not well understood. Aim To systematically explore the literature on the gastric microbiota in health and disease as well as the gut microbiota after bariatric surgery. Methods A systematic search of online bibliographic databases MEDLINE/EMBASE was performed between 1966 and February 2019 with screening in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Randomised controlled trials, cohort studies and observational studies were included if they reported next-generation sequencing derived microbiota analysis on gastric aspirate/tissue or stool samples (bariatric surgical outcomes). Results Sixty-five papers were eligible for inclusion. With the exception of H pylori-induced conditions, overarching gastric microbiota signatures of health or disease could not be determined. Gastric carcinogenesis induces a progressively altered microbiota with an enrichment of oral and intestinal taxa as well as significant changes in host gastric mucin expression. Proton pump inhibitors usage increases gastric microbiota richness. Bariatric surgery is associated with an increase in potentially pathogenic proteobacterial species in patient stool samples. Conclusion While H pylori remains the single most important risk factor for gastric disease, its capacity to shape the collective gastric microbiota remains to be fully elucidated. Further studies are needed to explore the intricate host/microbial and microbial/microbial interplay.
PB  - Wiley, Hoboken
T2  - Alimentary Pharmacology & Therapeutics
T1  - Systematic review: gastric microbiota in health and disease
EP  - 602
IS  - 6
SP  - 582
VL  - 51
DO  - 10.1111/apt.15650
ER  - 

@article{
author = "Rajilić-Stojanović, Mirjana and Figueiredo, Ceu and Smet, Annemieke and Hansen, Richard and Kupcinskas, Juozas and Rokkas, Theo and Andersen, Leif and Machado, Jose C. and Ianiro, Gianluca and Gasbarrini, Antonio and Leja, Marcis and Gisbert, Javier P. and Hold, Georgina L.",
year = "2020",
abstract = "Background Helicobacter pylori is the most infamous constituent of the gastric microbiota and its presence is the strongest risk factor for gastric cancer and other gastroduodenal diseases. Although historically the healthy stomach was considered a sterile organ, we now know it is colonised with a complex microbiota. However, its role in health and disease is not well understood. Aim To systematically explore the literature on the gastric microbiota in health and disease as well as the gut microbiota after bariatric surgery. Methods A systematic search of online bibliographic databases MEDLINE/EMBASE was performed between 1966 and February 2019 with screening in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Randomised controlled trials, cohort studies and observational studies were included if they reported next-generation sequencing derived microbiota analysis on gastric aspirate/tissue or stool samples (bariatric surgical outcomes). Results Sixty-five papers were eligible for inclusion. With the exception of H pylori-induced conditions, overarching gastric microbiota signatures of health or disease could not be determined. Gastric carcinogenesis induces a progressively altered microbiota with an enrichment of oral and intestinal taxa as well as significant changes in host gastric mucin expression. Proton pump inhibitors usage increases gastric microbiota richness. Bariatric surgery is associated with an increase in potentially pathogenic proteobacterial species in patient stool samples. Conclusion While H pylori remains the single most important risk factor for gastric disease, its capacity to shape the collective gastric microbiota remains to be fully elucidated. Further studies are needed to explore the intricate host/microbial and microbial/microbial interplay.",
publisher = "Wiley, Hoboken",
journal = "Alimentary Pharmacology & Therapeutics",
title = "Systematic review: gastric microbiota in health and disease",
pages = "602-582",
number = "6",
volume = "51",
doi = "10.1111/apt.15650"
}

Rajilić-Stojanović, M., Figueiredo, C., Smet, A., Hansen, R., Kupcinskas, J., Rokkas, T., Andersen, L., Machado, J. C., Ianiro, G., Gasbarrini, A., Leja, M., Gisbert, J. P.,& Hold, G. L.. (2020). Systematic review: gastric microbiota in health and disease. in Alimentary Pharmacology & Therapeutics
Wiley, Hoboken., 51(6), 582-602.
https://doi.org/10.1111/apt.15650

Rajilić-Stojanović M, Figueiredo C, Smet A, Hansen R, Kupcinskas J, Rokkas T, Andersen L, Machado JC, Ianiro G, Gasbarrini A, Leja M, Gisbert JP, Hold GL. Systematic review: gastric microbiota in health and disease. in Alimentary Pharmacology & Therapeutics. 2020;51(6):582-602.
doi:10.1111/apt.15650 .

Rajilić-Stojanović, Mirjana, Figueiredo, Ceu, Smet, Annemieke, Hansen, Richard, Kupcinskas, Juozas, Rokkas, Theo, Andersen, Leif, Machado, Jose C., Ianiro, Gianluca, Gasbarrini, Antonio, Leja, Marcis, Gisbert, Javier P., Hold, Georgina L., "Systematic review: gastric microbiota in health and disease" in Alimentary Pharmacology & Therapeutics, 51, no. 6 (2020):582-602,
https://doi.org/10.1111/apt.15650 . .

TY  - JOUR
AU  - Barbara, Giovanni
AU  - Grover, Madhusudan
AU  - Bercik, Premysl
AU  - Corsetti, Maura
AU  - Ghoshal, Uday C.
AU  - Ohman, Lena
AU  - Rajilić-Stojanović, Mirjana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4322
AB  - BACKGROUND & AIMS: The existence of postinfection irritable bowel syndrome (PI-IBS) has been substantiated by epidemiology studies conducted in diverse geographic and clinical settings. However, the available evidence has not been well summarized, and there is little guidance for diagnosis and treatment of PI-IBS. The ROME Foundation has produced a working team report to summarize the available evidence on the pathophysiology of PI-IBS and provide guidance for diagnosis and treatment, based on findings reported in the literature and clinical experience. METHODS: The working team conducted an evidence-based review of publication databases for articles describing the clinical features (diagnosis), pathophysiology (intestinal sensorimotor function, microbiota, immune dysregulation, barrier dysfunction, enteroendocrine pathways, and genetics), and animal models of PI-IBS. We used a Delphi-based consensus system to create guidelines for management of PI-IBS and a developed treatment algorithm based on published findings and experiences of team members. RESULTS: PI-IBS develops in about 10% of patients with infectious enteritis. Risk factors include female sex, younger age, psychological distress during or before acute gastroenteritis, and severity of the acute episode. The pathogenesis of PI-PBS appears to involve changes in the intestinal microbiome as well as epithelial, serotonergic, and immune system factors. However, these mechanisms are incompletely understood. There are no evidence-based, effective pharmacologic strategies for treatment of PI-IBS. We provide a consensus-based treatment algorithm, based on clinical presentation and potential disease mechanisms. CONCLUSIONS: Based on a systematic review of the literature and team experience, we summarize the clinical features, pathophysiology (from animal models and human studies), and progression of PI-IBS. Based on these findings, we present an algorithm for diagnosis and treatment of PI-IBS based on team consensus. We also propose areas for future investigation.
PB  - W B Saunders Co-Elsevier Inc, Philadelphia
T2  - Gastroenterology
T1  - Rome Foundation Working Team Report on Post-Infection Irritable Bowel Syndrome
EP  - +
IS  - 1
SP  - 46
VL  - 156
DO  - 10.1053/j.gastro.2018.07.011
ER  - 

@article{
author = "Barbara, Giovanni and Grover, Madhusudan and Bercik, Premysl and Corsetti, Maura and Ghoshal, Uday C. and Ohman, Lena and Rajilić-Stojanović, Mirjana",
year = "2019",
abstract = "BACKGROUND & AIMS: The existence of postinfection irritable bowel syndrome (PI-IBS) has been substantiated by epidemiology studies conducted in diverse geographic and clinical settings. However, the available evidence has not been well summarized, and there is little guidance for diagnosis and treatment of PI-IBS. The ROME Foundation has produced a working team report to summarize the available evidence on the pathophysiology of PI-IBS and provide guidance for diagnosis and treatment, based on findings reported in the literature and clinical experience. METHODS: The working team conducted an evidence-based review of publication databases for articles describing the clinical features (diagnosis), pathophysiology (intestinal sensorimotor function, microbiota, immune dysregulation, barrier dysfunction, enteroendocrine pathways, and genetics), and animal models of PI-IBS. We used a Delphi-based consensus system to create guidelines for management of PI-IBS and a developed treatment algorithm based on published findings and experiences of team members. RESULTS: PI-IBS develops in about 10% of patients with infectious enteritis. Risk factors include female sex, younger age, psychological distress during or before acute gastroenteritis, and severity of the acute episode. The pathogenesis of PI-PBS appears to involve changes in the intestinal microbiome as well as epithelial, serotonergic, and immune system factors. However, these mechanisms are incompletely understood. There are no evidence-based, effective pharmacologic strategies for treatment of PI-IBS. We provide a consensus-based treatment algorithm, based on clinical presentation and potential disease mechanisms. CONCLUSIONS: Based on a systematic review of the literature and team experience, we summarize the clinical features, pathophysiology (from animal models and human studies), and progression of PI-IBS. Based on these findings, we present an algorithm for diagnosis and treatment of PI-IBS based on team consensus. We also propose areas for future investigation.",
publisher = "W B Saunders Co-Elsevier Inc, Philadelphia",
journal = "Gastroenterology",
title = "Rome Foundation Working Team Report on Post-Infection Irritable Bowel Syndrome",
pages = "+-46",
number = "1",
volume = "156",
doi = "10.1053/j.gastro.2018.07.011"
}

Barbara, G., Grover, M., Bercik, P., Corsetti, M., Ghoshal, U. C., Ohman, L.,& Rajilić-Stojanović, M.. (2019). Rome Foundation Working Team Report on Post-Infection Irritable Bowel Syndrome. in Gastroenterology
W B Saunders Co-Elsevier Inc, Philadelphia., 156(1), 46-+.
https://doi.org/10.1053/j.gastro.2018.07.011

Barbara G, Grover M, Bercik P, Corsetti M, Ghoshal UC, Ohman L, Rajilić-Stojanović M. Rome Foundation Working Team Report on Post-Infection Irritable Bowel Syndrome. in Gastroenterology. 2019;156(1):46-+.
doi:10.1053/j.gastro.2018.07.011 .

Barbara, Giovanni, Grover, Madhusudan, Bercik, Premysl, Corsetti, Maura, Ghoshal, Uday C., Ohman, Lena, Rajilić-Stojanović, Mirjana, "Rome Foundation Working Team Report on Post-Infection Irritable Bowel Syndrome" in Gastroenterology, 156, no. 1 (2019):46-+,
https://doi.org/10.1053/j.gastro.2018.07.011 . .

TY  - CHAP
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević, Suzana
AU  - Golić, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6055
AB  - In the complex ecosystem of gut microbiota, lactic acid bacteria (LAB)+B represent prevalent, abundant, and highly important subpopulation. LAB+B are well-known for their health-promoting effects: they are found to positively influence gut microbiota diversity, modulate immune response and intestinal permeability, and produce bioactive or regulatory metabolites. Bifidobacteriaceae represent critical early colonizers of the human gut, and the depletion of this microbial group in infants is correlated with allergy development. LAB+B are considered as one of the first human gut colonizers and recent studies have shown that humans might be exposed to them even in the womb. The primary role of the human gut microbiota, as in other animals, is its contribution to digestion. Several types of health-promoting dietary polyphenols are able to boost the abundance of LAB+B in human trials, but also in in vitro tests and animal models. A molecular methods-based insight into gut microbiota diversity showed presence of its disturbed composition-dysbiosis-in various diseases.
PB  - CRC Press
T2  - Lactic Acid Bacteria
T1  - Lactic Acid Bacteria in the Gut
EP  - 407
SP  - 381
DO  - 10.1201/9780429057465-24
ER  - 

@inbook{
author = "Rajilić-Stojanović, Mirjana and Dimitrijević, Suzana and Golić, Nataša",
year = "2019",
abstract = "In the complex ecosystem of gut microbiota, lactic acid bacteria (LAB)+B represent prevalent, abundant, and highly important subpopulation. LAB+B are well-known for their health-promoting effects: they are found to positively influence gut microbiota diversity, modulate immune response and intestinal permeability, and produce bioactive or regulatory metabolites. Bifidobacteriaceae represent critical early colonizers of the human gut, and the depletion of this microbial group in infants is correlated with allergy development. LAB+B are considered as one of the first human gut colonizers and recent studies have shown that humans might be exposed to them even in the womb. The primary role of the human gut microbiota, as in other animals, is its contribution to digestion. Several types of health-promoting dietary polyphenols are able to boost the abundance of LAB+B in human trials, but also in in vitro tests and animal models. A molecular methods-based insight into gut microbiota diversity showed presence of its disturbed composition-dysbiosis-in various diseases.",
publisher = "CRC Press",
journal = "Lactic Acid Bacteria",
booktitle = "Lactic Acid Bacteria in the Gut",
pages = "407-381",
doi = "10.1201/9780429057465-24"
}

Rajilić-Stojanović, M., Dimitrijević, S.,& Golić, N.. (2019). Lactic Acid Bacteria in the Gut. in Lactic Acid Bacteria
CRC Press., 381-407.
https://doi.org/10.1201/9780429057465-24

Rajilić-Stojanović M, Dimitrijević S, Golić N. Lactic Acid Bacteria in the Gut. in Lactic Acid Bacteria. 2019;:381-407.
doi:10.1201/9780429057465-24 .

Rajilić-Stojanović, Mirjana, Dimitrijević, Suzana, Golić, Nataša, "Lactic Acid Bacteria in the Gut" in Lactic Acid Bacteria (2019):381-407,
https://doi.org/10.1201/9780429057465-24 . .

TY  - CHAP
AU  - Bojić, Svetlana
AU  - Tolinački, Maja
AU  - Rajilić-Stojanović, Mirjana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6129
AB  - Gut microbiota is an exceptionally diverse, variable and dense microbial ecosystem that has been extensively studied in recent years. Numerous technological developments have facilitated the rapid progress of the field by the improvement of old and the emergence of new methods. Most of the available methods are based on the sequence of the small subunit ribosomal RNA and are suitable for assessment of gut microbiota composition. Others, such as metagenomics and metabolomics, provide information about gut microbiota function. In this chapter we systematically present the most widely used approaches including cultivation/culturomics, quantitative and profiling small subunit ribosomal RNA-based methods, sequencing technologies with specific focus on bioinformatics and data processing, finishing with omics that facilitate functional profiling. The advantages and limitations of each technology are presented, while examples of successful applications provide evidence that different scientific questions call for the application of different techniques.
T2  - Microbiota y probióticos en gastroenterología
T1  - Techniques for the study of microbiota
EP  - 20
SP  - 11
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6129
ER  - 

@inbook{
author = "Bojić, Svetlana and Tolinački, Maja and Rajilić-Stojanović, Mirjana",
year = "2019",
abstract = "Gut microbiota is an exceptionally diverse, variable and dense microbial ecosystem that has been extensively studied in recent years. Numerous technological developments have facilitated the rapid progress of the field by the improvement of old and the emergence of new methods. Most of the available methods are based on the sequence of the small subunit ribosomal RNA and are suitable for assessment of gut microbiota composition. Others, such as metagenomics and metabolomics, provide information about gut microbiota function. In this chapter we systematically present the most widely used approaches including cultivation/culturomics, quantitative and profiling small subunit ribosomal RNA-based methods, sequencing technologies with specific focus on bioinformatics and data processing, finishing with omics that facilitate functional profiling. The advantages and limitations of each technology are presented, while examples of successful applications provide evidence that different scientific questions call for the application of different techniques.",
journal = "Microbiota y probióticos en gastroenterología",
booktitle = "Techniques for the study of microbiota",
pages = "20-11",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6129"
}

Bojić, S., Tolinački, M.,& Rajilić-Stojanović, M.. (2019). Techniques for the study of microbiota. in Microbiota y probióticos en gastroenterología, 11-20.
https://hdl.handle.net/21.15107/rcub_technorep_6129

Bojić S, Tolinački M, Rajilić-Stojanović M. Techniques for the study of microbiota. in Microbiota y probióticos en gastroenterología. 2019;:11-20.
https://hdl.handle.net/21.15107/rcub_technorep_6129 .

Bojić, Svetlana, Tolinački, Maja, Rajilić-Stojanović, Mirjana, "Techniques for the study of microbiota" in Microbiota y probióticos en gastroenterología (2019):11-20,
https://hdl.handle.net/21.15107/rcub_technorep_6129 .

TY  - JOUR
AU  - Radisavljević, Anđela
AU  - Stojanović, Dušica
AU  - Perišić, Srđan
AU  - Đokić, Veljko
AU  - Radojević, Vesna
AU  - Rajilić-Stojanović, Mirjana
AU  - Uskoković, Petar
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3931
AB  - Antibiotic containing polycaprolactone (PCL) fibers were produced by using three electrospinning methods: blend, emulsion and co-axial electrospinning (labeled as S1, S2 and S3, respectively). The profiles of drug release from three different systems were studied and antimicrobial properties of produced materials were evaluated. Morphology of the produced fibers was characterized and revealed that cefazolin-loaded PCL fibers had smaller diameter compared to neat PCL fibers, while the chemical interaction between the antibiotic and PCL showed that cefazolin neither had reacted with PCL phase, nor had degraded during the electrospinning process. The crystallinity and thermal characterization of fabricated fibers showed that the addition of cefazolin decreased the crystallinity of PCL. The results of the drug release behavior of the blend and co-axial electrospun fibers was on a higher level (similar to 68% and similar to 43%, respectively) compared to the emulsion electrospun fibers (similar to 5%), after a period of 30 days. The obtained data had the best fitting with the first order model and the Higuchi model, while the Korsmeyer-Peppas model showed a Pseudo-Fickian diffusion of the drug. Antibacterial evaluations showed that cefazolin-loaded PCL fibers had better effects on Staphylococcus aureus compared to Escherichia coll. during the treatment period and that the effect of the emulsion fibers was notably weaker than the other two studied systems. The aim of the study was to test different systems for control drug release of different dynamics, which will be applied for prevent bacterial accumulation when indwelling urinary catheters, applied for different periods of time.
PB  - Elsevier, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect
EP  - 36
SP  - 26
VL  - 124
DO  - 10.1016/j.ejps.2018.08.023
ER  - 

@article{
author = "Radisavljević, Anđela and Stojanović, Dušica and Perišić, Srđan and Đokić, Veljko and Radojević, Vesna and Rajilić-Stojanović, Mirjana and Uskoković, Petar",
year = "2018",
abstract = "Antibiotic containing polycaprolactone (PCL) fibers were produced by using three electrospinning methods: blend, emulsion and co-axial electrospinning (labeled as S1, S2 and S3, respectively). The profiles of drug release from three different systems were studied and antimicrobial properties of produced materials were evaluated. Morphology of the produced fibers was characterized and revealed that cefazolin-loaded PCL fibers had smaller diameter compared to neat PCL fibers, while the chemical interaction between the antibiotic and PCL showed that cefazolin neither had reacted with PCL phase, nor had degraded during the electrospinning process. The crystallinity and thermal characterization of fabricated fibers showed that the addition of cefazolin decreased the crystallinity of PCL. The results of the drug release behavior of the blend and co-axial electrospun fibers was on a higher level (similar to 68% and similar to 43%, respectively) compared to the emulsion electrospun fibers (similar to 5%), after a period of 30 days. The obtained data had the best fitting with the first order model and the Higuchi model, while the Korsmeyer-Peppas model showed a Pseudo-Fickian diffusion of the drug. Antibacterial evaluations showed that cefazolin-loaded PCL fibers had better effects on Staphylococcus aureus compared to Escherichia coll. during the treatment period and that the effect of the emulsion fibers was notably weaker than the other two studied systems. The aim of the study was to test different systems for control drug release of different dynamics, which will be applied for prevent bacterial accumulation when indwelling urinary catheters, applied for different periods of time.",
publisher = "Elsevier, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect",
pages = "36-26",
volume = "124",
doi = "10.1016/j.ejps.2018.08.023"
}

Radisavljević, A., Stojanović, D., Perišić, S., Đokić, V., Radojević, V., Rajilić-Stojanović, M.,& Uskoković, P.. (2018). Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect. in European Journal of Pharmaceutical Sciences
Elsevier, Amsterdam., 124, 26-36.
https://doi.org/10.1016/j.ejps.2018.08.023

Radisavljević A, Stojanović D, Perišić S, Đokić V, Radojević V, Rajilić-Stojanović M, Uskoković P. Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect. in European Journal of Pharmaceutical Sciences. 2018;124:26-36.
doi:10.1016/j.ejps.2018.08.023 .

Radisavljević, Anđela, Stojanović, Dušica, Perišić, Srđan, Đokić, Veljko, Radojević, Vesna, Rajilić-Stojanović, Mirjana, Uskoković, Petar, "Cefazolin-loaded polycaprolactone fibers produced via different electrospinning methods: Characterization, drug release and antibacterial effect" in European Journal of Pharmaceutical Sciences, 124 (2018):26-36,
https://doi.org/10.1016/j.ejps.2018.08.023 . .

TY  - JOUR
AU  - Rajilić-Stojanović, Mirjana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3762
AB  - Humans live in a close association with a complex microbial ecosystem that is collectively termed microbiota. Microbiota is concentrated in the last part of the gastrointestinal tract, where it ferments undigested food residues and produces various metabolites that have a systemic influence on human physiology. The composition of microbiota is influenced by genetic and environmental factors that shape a unique ecosystem in each human. Despite considerable compositional variations, the functional output of the ecosystem is highly similar in all healthy individuals. Although it is still not possible to define normal microbiota, comparisons between patients suffering from a number of chronic diseases with unknown etiology and controls have shown that patients have specific, dysbiotic microbiota composition. While it still remains to be determined if the microbiota dysbiosis is an etiological factor or a consequence of a disease, these findings have initiated intensive microbiota research. Numerous proven functions of microbiota include contribution to the digestion, synthesis of essential metabolites (vitamins and amino acids) and the intensive crosstalk with the immune, the endocrine and the nervous system. Even though this field of research is still in an early stage, it is clear that microbial metabolites can have various effects on humans including direct beneficial effect, toxicity, but also more subtle interactions since microbial products can act as immune regulating or neuro-signaling molecules. The complex network of bidirectional interactions between microbiota and humans is unexplored but highly potent for development of novel strategies to preserve and improve health.
PB  - University of Rijeka
T2  - Psihologijske Teme
T1  - Gut microbiota and its role in human health
EP  - 32
IS  - 1
SP  - 17
VL  - 27
DO  - 10.31820/pt.27.1.2
ER  - 

@article{
author = "Rajilić-Stojanović, Mirjana",
year = "2018",
abstract = "Humans live in a close association with a complex microbial ecosystem that is collectively termed microbiota. Microbiota is concentrated in the last part of the gastrointestinal tract, where it ferments undigested food residues and produces various metabolites that have a systemic influence on human physiology. The composition of microbiota is influenced by genetic and environmental factors that shape a unique ecosystem in each human. Despite considerable compositional variations, the functional output of the ecosystem is highly similar in all healthy individuals. Although it is still not possible to define normal microbiota, comparisons between patients suffering from a number of chronic diseases with unknown etiology and controls have shown that patients have specific, dysbiotic microbiota composition. While it still remains to be determined if the microbiota dysbiosis is an etiological factor or a consequence of a disease, these findings have initiated intensive microbiota research. Numerous proven functions of microbiota include contribution to the digestion, synthesis of essential metabolites (vitamins and amino acids) and the intensive crosstalk with the immune, the endocrine and the nervous system. Even though this field of research is still in an early stage, it is clear that microbial metabolites can have various effects on humans including direct beneficial effect, toxicity, but also more subtle interactions since microbial products can act as immune regulating or neuro-signaling molecules. The complex network of bidirectional interactions between microbiota and humans is unexplored but highly potent for development of novel strategies to preserve and improve health.",
publisher = "University of Rijeka",
journal = "Psihologijske Teme",
title = "Gut microbiota and its role in human health",
pages = "32-17",
number = "1",
volume = "27",
doi = "10.31820/pt.27.1.2"
}

Rajilić-Stojanović, M.. (2018). Gut microbiota and its role in human health. in Psihologijske Teme
University of Rijeka., 27(1), 17-32.
https://doi.org/10.31820/pt.27.1.2

Rajilić-Stojanović M. Gut microbiota and its role in human health. in Psihologijske Teme. 2018;27(1):17-32.
doi:10.31820/pt.27.1.2 .

Rajilić-Stojanović, Mirjana, "Gut microbiota and its role in human health" in Psihologijske Teme, 27, no. 1 (2018):17-32,
https://doi.org/10.31820/pt.27.1.2 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Milutinović, Milica
AU  - Mihajlovski, Katarina
AU  - Jović, Jelena M.
AU  - Nastasijević, Branislav J.
AU  - Rajilić-Stojanović, Mirjana
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3948
AB  - In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusariurn graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment
EP  - 78
SP  - 71
VL  - 120
DO  - 10.1016/j.micpath.2018.04.056
ER  - 

@article{
author = "Radovanović, Neda and Milutinović, Milica and Mihajlovski, Katarina and Jović, Jelena M. and Nastasijević, Branislav J. and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "In the current study, the biocontrol potential of a novel strain Bacillus sp. PPM3 isolated from marine sediment from the Red Sea in Hurghada, Egypt is recognized. This novel strain was selected out of 32 isolates based on its ability to suppress the growth of four plant pathogenic fungi: Aspergillus flavus, Fusariurn graminearum, Mucor sp. and Alternaria sp. The new marine strain was identified and characterized by phenotypic and molecular approaches. The culture filtrate of Bacillus sp. PPM3 suppressed the growth and spore germination of all tested fungi in vitro with the highest value of inhibition reported for Mucor sp. (97.5%). The antifungal effect of the culture filtrate from the strain PPM3 was due to production of highly stable secondary metabolites resistant to extreme pH, temperature and enzymatic treatments. A PCR analysis confirmed the expression of genes involved in the synthesis of antifungal lipopeptides: iturin, bacillomycin D, mycosubtilin and surfactin. In a greenhouse experiment strain PPM3 effectively reduced disease incidence of F. graminearum in maize plants and displayed additional plant growth stimulating effect. The results show that novel marine strain PPM3 could have a potential in commercial application as biocontrol agent for treatment of various plant diseases caused by soil-borne and postharvest pathogenic fungi.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment",
pages = "78-71",
volume = "120",
doi = "10.1016/j.micpath.2018.04.056"
}

Radovanović, N., Milutinović, M., Mihajlovski, K., Jović, J. M., Nastasijević, B. J., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2018). Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 120, 71-78.
https://doi.org/10.1016/j.micpath.2018.04.056

Radovanović N, Milutinović M, Mihajlovski K, Jović JM, Nastasijević BJ, Rajilić-Stojanović M, Dimitrijević-Branković S. Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment. in Microbial Pathogenesis. 2018;120:71-78.
doi:10.1016/j.micpath.2018.04.056 .

Radovanović, Neda, Milutinović, Milica, Mihajlovski, Katarina, Jović, Jelena M., Nastasijević, Branislav J., Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Biocontrol and plant stimulating potential of novel strain Bacillus sp. PPM3 isolated from marine sediment" in Microbial Pathogenesis, 120 (2018):71-78,
https://doi.org/10.1016/j.micpath.2018.04.056 . .

TY  - JOUR
AU  - Rosania, Rosa
AU  - Von Arnim, Ulrike
AU  - Link, Alexander
AU  - Rajilić-Stojanović, Mirjana
AU  - Franck, Caspar
AU  - Canbay, Ali
AU  - Malfertheiner, Peter
AU  - Venerito, Marino
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4013
AB  - Background & Aims: A negative association between H. pylori and inflammatory bowel disease (IBD) has been previously reported. There were also case reports suggesting a new onset of IBD 6-12 months after H. pylori eradication therapy. In a case-control study we investigated whether previous H. pylori eradication therapy was associated with the risk of developing IBD. Methods: IBD outpatients with both Crohn's disease (CD) and ulcerative colitis (UC) were enrolled. Age- and sex-matched blood donors served as controls in a 1:2 fashion. Information on demographics, medical history, previous H. pylori infection and eradication therapy was recorded. Serum samples for H. pylori serology testing (anti-H. pylori-IgG and anti-CagA-IgG) were obtained. Controls that received H. pylori eradication therapy during the 12 months previous to enrollment were excluded. Results: Overall, 127 IBD patients (CD N= 90; UC N= 37) and 254 controls were enrolled. The prevalence of H. pylori infection (positive H. pylori serology and/or previous eradication) in IBD patients and controls was 11% and 23%, respectively (OR 0.4, 95% CI 0.21-0.74, p lt 0.003). Four patients (3%) developed IBD (3 MC and 1 CU) after receiving successful H. pylori eradication (latency 6-12 months). The rate of previous H. pylori eradication therapy in patents who successively developed IBD was lower but not statistically different from that observed in the control group (OR 0.43, 95% CI 0.14-1.29, p=0.16). Conclusions: In our study previous H. pylori eradication therapy was not associated with the onset of IBD. Whether in a subgroup of patients, H. pylori eradication therapy may trigger a latent IBD, cannot be excluded.
PB  - Medical Univ Press, Cluj-Napoca
T2  - Journal of Gastrointestinal and Liver Diseases
T1  - Helicobacter Pylori Eradication Therapy is Not Associated with the Onset of Inflammatory Bowel Diseases. A Case-Control Study
EP  - 125
IS  - 2
SP  - 119
VL  - 27
DO  - 10.15403/jgld.2014.1121.272.hpy
ER  - 

@article{
author = "Rosania, Rosa and Von Arnim, Ulrike and Link, Alexander and Rajilić-Stojanović, Mirjana and Franck, Caspar and Canbay, Ali and Malfertheiner, Peter and Venerito, Marino",
year = "2018",
abstract = "Background & Aims: A negative association between H. pylori and inflammatory bowel disease (IBD) has been previously reported. There were also case reports suggesting a new onset of IBD 6-12 months after H. pylori eradication therapy. In a case-control study we investigated whether previous H. pylori eradication therapy was associated with the risk of developing IBD. Methods: IBD outpatients with both Crohn's disease (CD) and ulcerative colitis (UC) were enrolled. Age- and sex-matched blood donors served as controls in a 1:2 fashion. Information on demographics, medical history, previous H. pylori infection and eradication therapy was recorded. Serum samples for H. pylori serology testing (anti-H. pylori-IgG and anti-CagA-IgG) were obtained. Controls that received H. pylori eradication therapy during the 12 months previous to enrollment were excluded. Results: Overall, 127 IBD patients (CD N= 90; UC N= 37) and 254 controls were enrolled. The prevalence of H. pylori infection (positive H. pylori serology and/or previous eradication) in IBD patients and controls was 11% and 23%, respectively (OR 0.4, 95% CI 0.21-0.74, p lt 0.003). Four patients (3%) developed IBD (3 MC and 1 CU) after receiving successful H. pylori eradication (latency 6-12 months). The rate of previous H. pylori eradication therapy in patents who successively developed IBD was lower but not statistically different from that observed in the control group (OR 0.43, 95% CI 0.14-1.29, p=0.16). Conclusions: In our study previous H. pylori eradication therapy was not associated with the onset of IBD. Whether in a subgroup of patients, H. pylori eradication therapy may trigger a latent IBD, cannot be excluded.",
publisher = "Medical Univ Press, Cluj-Napoca",
journal = "Journal of Gastrointestinal and Liver Diseases",
title = "Helicobacter Pylori Eradication Therapy is Not Associated with the Onset of Inflammatory Bowel Diseases. A Case-Control Study",
pages = "125-119",
number = "2",
volume = "27",
doi = "10.15403/jgld.2014.1121.272.hpy"
}

Rosania, R., Von Arnim, U., Link, A., Rajilić-Stojanović, M., Franck, C., Canbay, A., Malfertheiner, P.,& Venerito, M.. (2018). Helicobacter Pylori Eradication Therapy is Not Associated with the Onset of Inflammatory Bowel Diseases. A Case-Control Study. in Journal of Gastrointestinal and Liver Diseases
Medical Univ Press, Cluj-Napoca., 27(2), 119-125.
https://doi.org/10.15403/jgld.2014.1121.272.hpy

Rosania R, Von Arnim U, Link A, Rajilić-Stojanović M, Franck C, Canbay A, Malfertheiner P, Venerito M. Helicobacter Pylori Eradication Therapy is Not Associated with the Onset of Inflammatory Bowel Diseases. A Case-Control Study. in Journal of Gastrointestinal and Liver Diseases. 2018;27(2):119-125.
doi:10.15403/jgld.2014.1121.272.hpy .

Rosania, Rosa, Von Arnim, Ulrike, Link, Alexander, Rajilić-Stojanović, Mirjana, Franck, Caspar, Canbay, Ali, Malfertheiner, Peter, Venerito, Marino, "Helicobacter Pylori Eradication Therapy is Not Associated with the Onset of Inflammatory Bowel Diseases. A Case-Control Study" in Journal of Gastrointestinal and Liver Diseases, 27, no. 2 (2018):119-125,
https://doi.org/10.15403/jgld.2014.1121.272.hpy . .