TY  - JOUR
AU  - Glišić, Ivana
AU  - Đorđević, Milena
AU  - Milošević, Nebojša
AU  - Radičević, Sanja
AU  - Marić, Slađana
AU  - Cerović, Radosav
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6807
AB  - The development of European plum (Prunus domestica L.) cultivars is a crucial part of the 70-year-long tradition of breeding work on temperate zone fruit species at the Fruit Research Institute, Čačak, Republic of Serbia. Since its establishment to date, 18 plum cultivars of worldwide importance have been named and released. During the evaluation of newly bred plum genotypes, particular attention has been paid to the study of their self-(in)compatibility status, which is very important both for growers and breeders. Therefore, the objective of this study was to determine the self-(in)compatibility status of the recently released plum cultivars ‘Divna’, ‘Lana’ and ‘Petra’. The 2‑year (2018/19) study included: assessment of in vitro pollen germination on agarose-sucrose medium, observation of pollen tube growth in vivo in pistils under self- and open-pollination modes using fluorescence microscopy and determination of fruit set. In addition, the effect of temperature on the progamic phase was evaluated. In vitro pollen germination had high values, indicating the good in vivo pollen behaviour. High values of the parameters of pollen tube growth in vivo and fruit set in both self- and open-pollination variants were obtained in ‘Divna’ and ‘Petra’. On the other hand, pollen tube growth inhibition was observed in the upper part of the styles after self-pollination of ‘Lana’. Consequently, in the base of the style and in the ovary no tubes were noticed and no fruit set was recorded. Based on the overall results, ‘Lana’ can be characterized as self-incompatible, whilst ‘Divna’ and ‘Petra’ are self-compatible plum cultivars. The influence of air temperatures on the reproductive process and fertilization success was specific to each genotype.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Erwerbs-Obstbau
T1  - Reproductive Behaviour of New European Plum Cultivars ‘Lana’, ‘Divna’ and ‘Petra’
DO  - 10.1007/s10341-023-00972-y
ER  - 

@article{
author = "Glišić, Ivana and Đorđević, Milena and Milošević, Nebojša and Radičević, Sanja and Marić, Slađana and Cerović, Radosav",
year = "2023",
abstract = "The development of European plum (Prunus domestica L.) cultivars is a crucial part of the 70-year-long tradition of breeding work on temperate zone fruit species at the Fruit Research Institute, Čačak, Republic of Serbia. Since its establishment to date, 18 plum cultivars of worldwide importance have been named and released. During the evaluation of newly bred plum genotypes, particular attention has been paid to the study of their self-(in)compatibility status, which is very important both for growers and breeders. Therefore, the objective of this study was to determine the self-(in)compatibility status of the recently released plum cultivars ‘Divna’, ‘Lana’ and ‘Petra’. The 2‑year (2018/19) study included: assessment of in vitro pollen germination on agarose-sucrose medium, observation of pollen tube growth in vivo in pistils under self- and open-pollination modes using fluorescence microscopy and determination of fruit set. In addition, the effect of temperature on the progamic phase was evaluated. In vitro pollen germination had high values, indicating the good in vivo pollen behaviour. High values of the parameters of pollen tube growth in vivo and fruit set in both self- and open-pollination variants were obtained in ‘Divna’ and ‘Petra’. On the other hand, pollen tube growth inhibition was observed in the upper part of the styles after self-pollination of ‘Lana’. Consequently, in the base of the style and in the ovary no tubes were noticed and no fruit set was recorded. Based on the overall results, ‘Lana’ can be characterized as self-incompatible, whilst ‘Divna’ and ‘Petra’ are self-compatible plum cultivars. The influence of air temperatures on the reproductive process and fertilization success was specific to each genotype.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Erwerbs-Obstbau",
title = "Reproductive Behaviour of New European Plum Cultivars ‘Lana’, ‘Divna’ and ‘Petra’",
doi = "10.1007/s10341-023-00972-y"
}

Glišić, I., Đorđević, M., Milošević, N., Radičević, S., Marić, S.,& Cerović, R.. (2023). Reproductive Behaviour of New European Plum Cultivars ‘Lana’, ‘Divna’ and ‘Petra’. in Erwerbs-Obstbau
Springer Science and Business Media Deutschland GmbH..
https://doi.org/10.1007/s10341-023-00972-y

Glišić I, Đorđević M, Milošević N, Radičević S, Marić S, Cerović R. Reproductive Behaviour of New European Plum Cultivars ‘Lana’, ‘Divna’ and ‘Petra’. in Erwerbs-Obstbau. 2023;.
doi:10.1007/s10341-023-00972-y .

Glišić, Ivana, Đorđević, Milena, Milošević, Nebojša, Radičević, Sanja, Marić, Slađana, Cerović, Radosav, "Reproductive Behaviour of New European Plum Cultivars ‘Lana’, ‘Divna’ and ‘Petra’" in Erwerbs-Obstbau (2023),
https://doi.org/10.1007/s10341-023-00972-y . .

TY  - JOUR
AU  - Milošević, Nebojša
AU  - Glišić, Ivana
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Marić, Slađana
AU  - Milošević, Tomo
AU  - Nikolić, Dragan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6575
AB  - Pollination and temperature are predominant factors affecting the progamic phase of sexual plant reproduction. In light of climate change and the emergence of increasing temperatures, new knowledge is necessary to encourage fruit breeding programmes to develop cultivars adapted to these changes, as well as to find the best incompatibility relationships with the aim of achieving high fruit quality and yield. This research was conducted to reveal the effect of pollination treatments and temperature on the progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars (‘Jojo’, ‘Hanita’ and ‘Katinka’) resistant/tolerant to Sharka virus using fluorescent microscopy. Reproductive parameters (flowering overlapping, pollen germination, number of pollen tubes in the upper part of the style and locule of the ovary, dynamics of pollen tubes growth and initial and final fruit set) were investigated in vivo under different pollination treatments (cross, open and self-pollination). The influence of different temperature regimes (20 °C, 23 °C and 26 °C) on the progamic phase was also observed under cross and self-pollination. The pollination treatments significantly influenced the number of pollen tubes in the style and ovary, as well as dynamics of pollen tubes in different regions of pistil. Pollen tubes showed better growth in different crossing combinations compared to open and self-pollination. Higher temperatures accelerated pollen tube growth and reduced the time they need to reach ovules. Generally, ‘Jojo’ appeared to be the best pollenizer among the examined cultivars, while ‘Katinka’ exhibited the best response of pollen tube growth to various temperature regimes. The differences observed between growth of the pollen tubes in each genotype-temperature combination revealed that certain crossing combinations were better adapted to higher temperatures so this can be taken into account as one of the adjustments to climate change. The ability of cultivars to respond to high temperatures in the progamic phase can also be helpful for breeding programs in developing new cultivars better adapted to different temperature conditions.
PB  - Elsevier B.V.
T2  - European Journal of Agronomy
T1  - Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus
SP  - 126909
VL  - 149
DO  - 10.1016/j.eja.2023.126909
ER  - 

@article{
author = "Milošević, Nebojša and Glišić, Ivana and Đorđević, Milena and Cerović, Radosav and Radičević, Sanja and Marić, Slađana and Milošević, Tomo and Nikolić, Dragan",
year = "2023",
abstract = "Pollination and temperature are predominant factors affecting the progamic phase of sexual plant reproduction. In light of climate change and the emergence of increasing temperatures, new knowledge is necessary to encourage fruit breeding programmes to develop cultivars adapted to these changes, as well as to find the best incompatibility relationships with the aim of achieving high fruit quality and yield. This research was conducted to reveal the effect of pollination treatments and temperature on the progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars (‘Jojo’, ‘Hanita’ and ‘Katinka’) resistant/tolerant to Sharka virus using fluorescent microscopy. Reproductive parameters (flowering overlapping, pollen germination, number of pollen tubes in the upper part of the style and locule of the ovary, dynamics of pollen tubes growth and initial and final fruit set) were investigated in vivo under different pollination treatments (cross, open and self-pollination). The influence of different temperature regimes (20 °C, 23 °C and 26 °C) on the progamic phase was also observed under cross and self-pollination. The pollination treatments significantly influenced the number of pollen tubes in the style and ovary, as well as dynamics of pollen tubes in different regions of pistil. Pollen tubes showed better growth in different crossing combinations compared to open and self-pollination. Higher temperatures accelerated pollen tube growth and reduced the time they need to reach ovules. Generally, ‘Jojo’ appeared to be the best pollenizer among the examined cultivars, while ‘Katinka’ exhibited the best response of pollen tube growth to various temperature regimes. The differences observed between growth of the pollen tubes in each genotype-temperature combination revealed that certain crossing combinations were better adapted to higher temperatures so this can be taken into account as one of the adjustments to climate change. The ability of cultivars to respond to high temperatures in the progamic phase can also be helpful for breeding programs in developing new cultivars better adapted to different temperature conditions.",
publisher = "Elsevier B.V.",
journal = "European Journal of Agronomy",
title = "Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus",
pages = "126909",
volume = "149",
doi = "10.1016/j.eja.2023.126909"
}

Milošević, N., Glišić, I., Đorđević, M., Cerović, R., Radičević, S., Marić, S., Milošević, T.,& Nikolić, D.. (2023). Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus. in European Journal of Agronomy
Elsevier B.V.., 149, 126909.
https://doi.org/10.1016/j.eja.2023.126909

Milošević N, Glišić I, Đorđević M, Cerović R, Radičević S, Marić S, Milošević T, Nikolić D. Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus. in European Journal of Agronomy. 2023;149:126909.
doi:10.1016/j.eja.2023.126909 .

Milošević, Nebojša, Glišić, Ivana, Đorđević, Milena, Cerović, Radosav, Radičević, Sanja, Marić, Slađana, Milošević, Tomo, Nikolić, Dragan, "Influence of pollination treatments and temperature regimes on progamic phase and fruit set in three European plum (Prunus domestica L.) cultivars tollerant/resistant to Sharka virus" in European Journal of Agronomy, 149 (2023):126909,
https://doi.org/10.1016/j.eja.2023.126909 . .

TY  - JOUR
AU  - Fotirić-Aksić, Milica
AU  - Cerović, Radosav
AU  - Hjeltnes, Stein Harald
AU  - Meland, Mekjell
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5024
AB  - This study evaluated the effective pollination period (EPP) in four European plum (Prunus domestica L.) cultivars ('Mallard', 'Edda', 'Jubileum', and 'Reeves') during two years (2018-2019) under the environmental conditions in western Norway. The pollination of plum cultivars was carried out one, three, five, seven, and nine days after anthesis (DAA) with a pollen mix of two compatible cultivars ('Victoria' and 'Opal'). Initial, middle-season, and final fruit set was recorded after one month and two months after pollination and just before the harvest, respectively. On average from both years cultivar 'Jubileum' had the highest fruit set when pollinated one, three, five, seven, and nine DAA (33.23%, 30.83%, 8.47%, 3.08%, and 1.15%, respectively), which was more than two folds higher fruit set than in the other studied cultivars. Cultivar 'Jubileum' showed significantly reduced fruit set between pollination on five and nine DAA, while cultivars 'Mallard', 'Edda', and 'Reeves' had markedly reduced fruit set if pollinated three to five DAA, implying that the EPP in 'Jubileum' was five days while in the rest it was three days. Variation of weather conditions during the flowering period in both years did not have a major effect on the receptivity of stigmas in the studied plum cultivars, which means that the existing differences in the length of EPP is maternal-genotype dependent.
PB  - MDPI
T2  - Horticulturae
T1  - The Effective Pollination Period of European Plum (Prunus domestica L.) Cultivars in Western Norway
IS  - 1
SP  - 55
VL  - 8
DO  - 10.3390/horticulturae8010055
ER  - 

@article{
author = "Fotirić-Aksić, Milica and Cerović, Radosav and Hjeltnes, Stein Harald and Meland, Mekjell",
year = "2022",
abstract = "This study evaluated the effective pollination period (EPP) in four European plum (Prunus domestica L.) cultivars ('Mallard', 'Edda', 'Jubileum', and 'Reeves') during two years (2018-2019) under the environmental conditions in western Norway. The pollination of plum cultivars was carried out one, three, five, seven, and nine days after anthesis (DAA) with a pollen mix of two compatible cultivars ('Victoria' and 'Opal'). Initial, middle-season, and final fruit set was recorded after one month and two months after pollination and just before the harvest, respectively. On average from both years cultivar 'Jubileum' had the highest fruit set when pollinated one, three, five, seven, and nine DAA (33.23%, 30.83%, 8.47%, 3.08%, and 1.15%, respectively), which was more than two folds higher fruit set than in the other studied cultivars. Cultivar 'Jubileum' showed significantly reduced fruit set between pollination on five and nine DAA, while cultivars 'Mallard', 'Edda', and 'Reeves' had markedly reduced fruit set if pollinated three to five DAA, implying that the EPP in 'Jubileum' was five days while in the rest it was three days. Variation of weather conditions during the flowering period in both years did not have a major effect on the receptivity of stigmas in the studied plum cultivars, which means that the existing differences in the length of EPP is maternal-genotype dependent.",
publisher = "MDPI",
journal = "Horticulturae",
title = "The Effective Pollination Period of European Plum (Prunus domestica L.) Cultivars in Western Norway",
number = "1",
pages = "55",
volume = "8",
doi = "10.3390/horticulturae8010055"
}

Fotirić-Aksić, M., Cerović, R., Hjeltnes, S. H.,& Meland, M.. (2022). The Effective Pollination Period of European Plum (Prunus domestica L.) Cultivars in Western Norway. in Horticulturae
MDPI., 8(1), 55.
https://doi.org/10.3390/horticulturae8010055

Fotirić-Aksić M, Cerović R, Hjeltnes SH, Meland M. The Effective Pollination Period of European Plum (Prunus domestica L.) Cultivars in Western Norway. in Horticulturae. 2022;8(1):55.
doi:10.3390/horticulturae8010055 .

Fotirić-Aksić, Milica, Cerović, Radosav, Hjeltnes, Stein Harald, Meland, Mekjell, "The Effective Pollination Period of European Plum (Prunus domestica L.) Cultivars in Western Norway" in Horticulturae, 8, no. 1 (2022):55,
https://doi.org/10.3390/horticulturae8010055 . .

TY  - JOUR
AU  - Ðorđević, Milena
AU  - Vujović, Tatjana
AU  - Cerović, Radosav
AU  - Glišić, Ivana
AU  - Milošević, Nebojša
AU  - Marić, Slađana
AU  - Radičević, Sanja
AU  - Fotirić Akšić, Milica
AU  - Meland, Mekjell
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5176
AB  - A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−20, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
T2  - Horticulturae
T2  - Horticulturae
T1  - In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods
IS  - 7
VL  - 8
DO  - 10.3390/horticulturae8070616
ER  - 

@article{
author = "Ðorđević, Milena and Vujović, Tatjana and Cerović, Radosav and Glišić, Ivana and Milošević, Nebojša and Marić, Slađana and Radičević, Sanja and Fotirić Akšić, Milica and Meland, Mekjell",
year = "2022",
abstract = "A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−20, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
journal = "Horticulturae, Horticulturae",
title = "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods",
number = "7",
volume = "8",
doi = "10.3390/horticulturae8070616"
}

Ðorđević, M., Vujović, T., Cerović, R., Glišić, I., Milošević, N., Marić, S., Radičević, S., Fotirić Akšić, M.,& Meland, M.. (2022). In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods. in Horticulturae, 8(7).
https://doi.org/10.3390/horticulturae8070616

Ðorđević M, Vujović T, Cerović R, Glišić I, Milošević N, Marić S, Radičević S, Fotirić Akšić M, Meland M. In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods. in Horticulturae. 2022;8(7).
doi:10.3390/horticulturae8070616 .

Ðorđević, Milena, Vujović, Tatjana, Cerović, Radosav, Glišić, Ivana, Milošević, Nebojša, Marić, Slađana, Radičević, Sanja, Fotirić Akšić, Milica, Meland, Mekjell, "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods" in Horticulturae, 8, no. 7 (2022),
https://doi.org/10.3390/horticulturae8070616 . .

TY  - JOUR
AU  - Cerović, Radosav
AU  - Fotirić-Akšić, Milica
AU  - Đorđević, Milena
AU  - Meland, Mekjell
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5117
AB  - Compatibility and synchrony between specialized tissues of the pistil, female gametophytes and male gametophytes, are necessary for successful pollination, fertilization, and fruit set in angiosperms. The aim of the present work was to study the development and viability of embryo sacs, as well as fertilization success, in relation to the fruit set of the cultivars ‘Mallard’, ‘Edda’, ‘Jubileum’, and ‘Reeves’, under specific Norwegian climatic conditions. Emasculated, unpollinated, and open-pollinated flowers were collected at the beginning of flowering, and on the 3rd, 6th, 9th, and 12th days after flowering, from all four plum cultivars over two years (2018/2019). Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under a light microscope. Results showed the existence of synchronization between successive phases in the development of the embryo sac and individual phases of flowering. All plum cultivars had higher percentages of viable embryo sacs, fertilized embryo sacs, and fruit set in 2018 than in 2019. These differences may be related to the very low temperatures during the post-full-flowering period in 2019, and to the low adaptation of some studied cultivars to unfavorable conditions. In our study, the cultivar ‘Jubileum’ showed the highest percentage of viable embryo sacs, fertilized embryo sacs, and fruit set compared to other cultivars, i.e., the best low-temperature adaptation.
PB  - MDPI
T2  - Plants
T1  - Viability of Embryo Sacs and Fruit Set in Different Plum (Prunus domestica L.) Cultivars Grown under Norwegian Climatic Conditions
IS  - 2
SP  - 219
VL  - 11
DO  - 10.3390/plants11020219
ER  - 

@article{
author = "Cerović, Radosav and Fotirić-Akšić, Milica and Đorđević, Milena and Meland, Mekjell",
year = "2022",
abstract = "Compatibility and synchrony between specialized tissues of the pistil, female gametophytes and male gametophytes, are necessary for successful pollination, fertilization, and fruit set in angiosperms. The aim of the present work was to study the development and viability of embryo sacs, as well as fertilization success, in relation to the fruit set of the cultivars ‘Mallard’, ‘Edda’, ‘Jubileum’, and ‘Reeves’, under specific Norwegian climatic conditions. Emasculated, unpollinated, and open-pollinated flowers were collected at the beginning of flowering, and on the 3rd, 6th, 9th, and 12th days after flowering, from all four plum cultivars over two years (2018/2019). Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under a light microscope. Results showed the existence of synchronization between successive phases in the development of the embryo sac and individual phases of flowering. All plum cultivars had higher percentages of viable embryo sacs, fertilized embryo sacs, and fruit set in 2018 than in 2019. These differences may be related to the very low temperatures during the post-full-flowering period in 2019, and to the low adaptation of some studied cultivars to unfavorable conditions. In our study, the cultivar ‘Jubileum’ showed the highest percentage of viable embryo sacs, fertilized embryo sacs, and fruit set compared to other cultivars, i.e., the best low-temperature adaptation.",
publisher = "MDPI",
journal = "Plants",
title = "Viability of Embryo Sacs and Fruit Set in Different Plum (Prunus domestica L.) Cultivars Grown under Norwegian Climatic Conditions",
number = "2",
pages = "219",
volume = "11",
doi = "10.3390/plants11020219"
}

Cerović, R., Fotirić-Akšić, M., Đorđević, M.,& Meland, M.. (2022). Viability of Embryo Sacs and Fruit Set in Different Plum (Prunus domestica L.) Cultivars Grown under Norwegian Climatic Conditions. in Plants
MDPI., 11(2), 219.
https://doi.org/10.3390/plants11020219

Cerović R, Fotirić-Akšić M, Đorđević M, Meland M. Viability of Embryo Sacs and Fruit Set in Different Plum (Prunus domestica L.) Cultivars Grown under Norwegian Climatic Conditions. in Plants. 2022;11(2):219.
doi:10.3390/plants11020219 .

Cerović, Radosav, Fotirić-Akšić, Milica, Đorđević, Milena, Meland, Mekjell, "Viability of Embryo Sacs and Fruit Set in Different Plum (Prunus domestica L.) Cultivars Grown under Norwegian Climatic Conditions" in Plants, 11, no. 2 (2022):219,
https://doi.org/10.3390/plants11020219 . .

TY  - JOUR
AU  - Leposavic, A.
AU  - Đorđevic, Milena
AU  - Cerović, Radosav
AU  - Radicevic, S.
AU  - Vujovic, T.
AU  - Durovic, D.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4833
AB  - The paper presents results of the three-year study of fertilization biology of 'Reka' highbush blueberry (Vaccinium corymbosum L.) under the cross-, open and self-pollination variants. Cross-pollination was performed using the pollen of 'Bluecrop', 'Duke', 'Nui' and 'Ozarkblue'. Pollen tube growth in vivo was monitored using fluorescent microscopy method. The folowing parameters were observed: the average number of pollen tubes in the upper part and base of the style, number of ovary locules with the penetration of pollen tubes, and polen tubes' growth dynamics through certain pistil parts. The highest number of pollen tubes in the upper part and base of the style, as well as the fastest penetration into the ovary locules were found in the open pollination variant. Significant decrease of pollen tubes number from the upper third to the base of the style has been recorded in all pollination variants, whereas in the self-pollination variant it was to the greatest extent. The results also indicated that pollen tube growth dynamics in the progamic phase of fertilization was significantly influenced by pollenizer genotype and environmental factors, temperature in particular. On the second day after pollination, pollen tubes penetrated the ovary locules in all pollination variants, implying that favourable period for fertilization in this cultivar was one to two days after flower opening. In self- and cross-pollination variants, dynamics of pollen tubes growth was similar, indicating that cultivar 'Reka', commonly grown in multi-varietal, could also be grown in mono-varietal plantations (without pollenizers).
T2  - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo
T1  - Fertilization biology of 'Reka' highbush blueberry
EP  - 284
SP  - 279
VL  - 1308
DO  - 10.17660/ActaHortic.2021.1308.39
ER  - 

@article{
author = "Leposavic, A. and Đorđevic, Milena and Cerović, Radosav and Radicevic, S. and Vujovic, T. and Durovic, D.",
year = "2021",
abstract = "The paper presents results of the three-year study of fertilization biology of 'Reka' highbush blueberry (Vaccinium corymbosum L.) under the cross-, open and self-pollination variants. Cross-pollination was performed using the pollen of 'Bluecrop', 'Duke', 'Nui' and 'Ozarkblue'. Pollen tube growth in vivo was monitored using fluorescent microscopy method. The folowing parameters were observed: the average number of pollen tubes in the upper part and base of the style, number of ovary locules with the penetration of pollen tubes, and polen tubes' growth dynamics through certain pistil parts. The highest number of pollen tubes in the upper part and base of the style, as well as the fastest penetration into the ovary locules were found in the open pollination variant. Significant decrease of pollen tubes number from the upper third to the base of the style has been recorded in all pollination variants, whereas in the self-pollination variant it was to the greatest extent. The results also indicated that pollen tube growth dynamics in the progamic phase of fertilization was significantly influenced by pollenizer genotype and environmental factors, temperature in particular. On the second day after pollination, pollen tubes penetrated the ovary locules in all pollination variants, implying that favourable period for fertilization in this cultivar was one to two days after flower opening. In self- and cross-pollination variants, dynamics of pollen tubes growth was similar, indicating that cultivar 'Reka', commonly grown in multi-varietal, could also be grown in mono-varietal plantations (without pollenizers).",
journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo",
title = "Fertilization biology of 'Reka' highbush blueberry",
pages = "284-279",
volume = "1308",
doi = "10.17660/ActaHortic.2021.1308.39"
}

Leposavic, A., Đorđevic, M., Cerović, R., Radicevic, S., Vujovic, T.,& Durovic, D.. (2021). Fertilization biology of 'Reka' highbush blueberry. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308, 279-284.
https://doi.org/10.17660/ActaHortic.2021.1308.39

Leposavic A, Đorđevic M, Cerović R, Radicevic S, Vujovic T, Durovic D. Fertilization biology of 'Reka' highbush blueberry. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo. 2021;1308:279-284.
doi:10.17660/ActaHortic.2021.1308.39 .

Leposavic, A., Đorđevic, Milena, Cerović, Radosav, Radicevic, S., Vujovic, T., Durovic, D., "Fertilization biology of 'Reka' highbush blueberry" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308 (2021):279-284,
https://doi.org/10.17660/ActaHortic.2021.1308.39 . .

TY  - JOUR
AU  - Radicevic, S.
AU  - Maric, S.
AU  - Cerović, Radosav
AU  - Đorđevic, Milena
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4832
AB  - The paper presents results of a two-year study of self-(in)compatibility in 'Karina', previously reported as a self-incompatible sweet cherry cultivar. After emasculation and bagging, pistils of 'Karina' were selfed and pollinated with the nominally cross-incompatible cultivars 'Germersdorfer 1' and 'Lambert' at the beginning of full flowering. In the same way, the pollen of 'Karina' was used to pollinate 'Germersdorfer 1' and 'Lambert'. PCR analysis to determine S-genotypes of 'Karina', 'Germersdorfer 1' and 'Lambert' using consensus and allele-specific primers for S-RNase resulted in patterns consistent with S3S4 genotype. After selfing, 'Karina' behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus and fruit set was recorded in both years. Pollen tubes penetrating the nucellus and fruit set were also recorded after pollination with 'Germersdorfer 1' and 'Lambert'. In contrast, 'Germersdorfer 1' and 'Lambert' did not set any fruit when pollinated with 'Karina'. Microscopic observation showed pollen tubes ending the growth in the upper and middle third of the style. The behaviour of 'Karina' after selfing, as well as its behaviour as the pollen donor/recipient in pollination with cultivars of the same S-allele constitution requires further molecular and genetic analysis.
T2  - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo
T1  - Investigation of S-incompatibility breakdown in 'Karina' sweet cherry
EP  - 60
SP  - 53
VL  - 1308
DO  - 10.17660/ActaHortic.2021.1308.9
ER  - 

@article{
author = "Radicevic, S. and Maric, S. and Cerović, Radosav and Đorđevic, Milena",
year = "2021",
abstract = "The paper presents results of a two-year study of self-(in)compatibility in 'Karina', previously reported as a self-incompatible sweet cherry cultivar. After emasculation and bagging, pistils of 'Karina' were selfed and pollinated with the nominally cross-incompatible cultivars 'Germersdorfer 1' and 'Lambert' at the beginning of full flowering. In the same way, the pollen of 'Karina' was used to pollinate 'Germersdorfer 1' and 'Lambert'. PCR analysis to determine S-genotypes of 'Karina', 'Germersdorfer 1' and 'Lambert' using consensus and allele-specific primers for S-RNase resulted in patterns consistent with S3S4 genotype. After selfing, 'Karina' behaved as self-compatible, since its pollen tubes reached the base of the style and ovary, penetrating the nucellus and fruit set was recorded in both years. Pollen tubes penetrating the nucellus and fruit set were also recorded after pollination with 'Germersdorfer 1' and 'Lambert'. In contrast, 'Germersdorfer 1' and 'Lambert' did not set any fruit when pollinated with 'Karina'. Microscopic observation showed pollen tubes ending the growth in the upper and middle third of the style. The behaviour of 'Karina' after selfing, as well as its behaviour as the pollen donor/recipient in pollination with cultivars of the same S-allele constitution requires further molecular and genetic analysis.",
journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo",
title = "Investigation of S-incompatibility breakdown in 'Karina' sweet cherry",
pages = "60-53",
volume = "1308",
doi = "10.17660/ActaHortic.2021.1308.9"
}

Radicevic, S., Maric, S., Cerović, R.,& Đorđevic, M.. (2021). Investigation of S-incompatibility breakdown in 'Karina' sweet cherry. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308, 53-60.
https://doi.org/10.17660/ActaHortic.2021.1308.9

Radicevic S, Maric S, Cerović R, Đorđevic M. Investigation of S-incompatibility breakdown in 'Karina' sweet cherry. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo. 2021;1308:53-60.
doi:10.17660/ActaHortic.2021.1308.9 .

Radicevic, S., Maric, S., Cerović, Radosav, Đorđevic, Milena, "Investigation of S-incompatibility breakdown in 'Karina' sweet cherry" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308 (2021):53-60,
https://doi.org/10.17660/ActaHortic.2021.1308.9 . .

TY  - JOUR
AU  - Vujovic, T.
AU  - Ruzic, D.
AU  - Cerović, Radosav
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4831
AB  - This paper investigates the influence of composition of vitrification solution on the regrowth capacity of apple 'Gala Must' (Malus x domestica Borkh.) cryopreserved by a vitrification technique. In vitro-grown shoot tips were precultured in the dark at 23 degrees C, in a liquid MS medium with two sucrose treatments (0.3 M for 15 h, then 0.7 M for 5 h). After loading at room temperature with solution containing 2 M glycerol and 0.4 M sucrose for 20 min, explants were dehydrated at 0 degrees C for 30, 40 and 50 min. Dehydration was done using either original PVS2 (13.7% w/v sucrose, 30% w/v glycerol, 15% w/v ethylene glycol, 15% w/v DMSO) or modified PVS2 solution (PVS A3 - 22.5% w/v sucrose, 37.5% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO). The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 40 and 85 and 20-40%, respectively. The highest values of both parameters were achieved with longest treatment duration. Dehydration with the PVS A3 solution resulted in considerably higher survival rates (68.2-90%), as well as higher regrowth rates (15-75%) after cryopreservation. Fifty-min treatment with this vitrification solution led to a significant increase in the percentage of regrowth (up to 75%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3 based vitrification technique for a long-term storage of this genotype.
T2  - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo
T1  - Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration
EP  - 40
SP  - 33
VL  - 1308
DO  - 10.17660/ActaHortic.2021.1308.6
ER  - 

@article{
author = "Vujovic, T. and Ruzic, D. and Cerović, Radosav",
year = "2021",
abstract = "This paper investigates the influence of composition of vitrification solution on the regrowth capacity of apple 'Gala Must' (Malus x domestica Borkh.) cryopreserved by a vitrification technique. In vitro-grown shoot tips were precultured in the dark at 23 degrees C, in a liquid MS medium with two sucrose treatments (0.3 M for 15 h, then 0.7 M for 5 h). After loading at room temperature with solution containing 2 M glycerol and 0.4 M sucrose for 20 min, explants were dehydrated at 0 degrees C for 30, 40 and 50 min. Dehydration was done using either original PVS2 (13.7% w/v sucrose, 30% w/v glycerol, 15% w/v ethylene glycol, 15% w/v DMSO) or modified PVS2 solution (PVS A3 - 22.5% w/v sucrose, 37.5% w/v glycerol, 15% w/v ethylene glycol and 15% w/v DMSO). The survival and regrowth of the cryopreserved shoot tips dehydrated with the original PVS2 solution ranged between 40 and 85 and 20-40%, respectively. The highest values of both parameters were achieved with longest treatment duration. Dehydration with the PVS A3 solution resulted in considerably higher survival rates (68.2-90%), as well as higher regrowth rates (15-75%) after cryopreservation. Fifty-min treatment with this vitrification solution led to a significant increase in the percentage of regrowth (up to 75%). After regrowth, shoots were successfully multiplied and rooted. These results prove the feasibility of the PVS A3 based vitrification technique for a long-term storage of this genotype.",
journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo",
title = "Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration",
pages = "40-33",
volume = "1308",
doi = "10.17660/ActaHortic.2021.1308.6"
}

Vujovic, T., Ruzic, D.,& Cerović, R.. (2021). Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308, 33-40.
https://doi.org/10.17660/ActaHortic.2021.1308.6

Vujovic T, Ruzic D, Cerović R. Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo. 2021;1308:33-40.
doi:10.17660/ActaHortic.2021.1308.6 .

Vujovic, T., Ruzic, D., Cerović, Radosav, "Cryopreservation of apple shoot tips by vitrification and subsequent plant regeneration" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308 (2021):33-40,
https://doi.org/10.17660/ActaHortic.2021.1308.6 . .

TY  - JOUR
AU  - Đorđevic, Milena
AU  - Cerović, Radosav
AU  - Radicevic, S.
AU  - Milosevic, N.
AU  - Glisic, I. S.
AU  - Maric, S.
AU  - Lukic, M.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4830
AB  - Plum cultivar 'Pozna Plava' was developed at the Fruit Research Institute, Cacak, and released as a new cultivar in 2008. This plum cultivar bears poorly and irregularly, resulting in very low fruit set. Therefore, the occurrence of specific behaviour of growing pollen tubes in the ovary was studied over a three-year period, in three pollination variants (self-, cross- and open). The cross-pollination was performed using the pollen of 'Cacanska Najbolja', 'Hanita' and 'Presenta' plum cultivars. Growth of pollen tubes was observed by means of fluorescent microscopy. The analysis was conducted taking into consideration whether or not the pollen tube has penetrated into the nucellus. Presence of specific behaviour of growing pollen tubes in the ovary was observed in all pollination variants. Atypical pollen tube growth was recorded in the locyle of the ovary as well as in the region of the obturator, the micropyle and the embryo sac. The largest percentage of atypical pollen tube growth in all pollination variants, before and after the penetration into the nucellus was recorded in the obturator and micropyle region. In the open pollination variant, the ovaries showing this specific behaviour of growing pollen tubes, were noted in only one year. Cross- and self-pollination variant showed the existence of specific behaviour of growing pollen tubes in all regions of the ovule, including the embryo sac (except in self-pollination). Only in cross-pollination variant, correlation-regression analysis has shown that specific behaviour of growing pollen tubes did affect the pollen tube penetrating into the nucellus.
T2  - Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo
T1  - Occurrence of specific behaviour of growing pollen tubes in the ovary of the 'Pozna Plava' plum cultivar
EP  - 12
SP  - 7
VL  - 1308
DO  - 10.17660/ActaHortic.2021.1308.2
ER  - 

@article{
author = "Đorđevic, Milena and Cerović, Radosav and Radicevic, S. and Milosevic, N. and Glisic, I. S. and Maric, S. and Lukic, M.",
year = "2021",
abstract = "Plum cultivar 'Pozna Plava' was developed at the Fruit Research Institute, Cacak, and released as a new cultivar in 2008. This plum cultivar bears poorly and irregularly, resulting in very low fruit set. Therefore, the occurrence of specific behaviour of growing pollen tubes in the ovary was studied over a three-year period, in three pollination variants (self-, cross- and open). The cross-pollination was performed using the pollen of 'Cacanska Najbolja', 'Hanita' and 'Presenta' plum cultivars. Growth of pollen tubes was observed by means of fluorescent microscopy. The analysis was conducted taking into consideration whether or not the pollen tube has penetrated into the nucellus. Presence of specific behaviour of growing pollen tubes in the ovary was observed in all pollination variants. Atypical pollen tube growth was recorded in the locyle of the ovary as well as in the region of the obturator, the micropyle and the embryo sac. The largest percentage of atypical pollen tube growth in all pollination variants, before and after the penetration into the nucellus was recorded in the obturator and micropyle region. In the open pollination variant, the ovaries showing this specific behaviour of growing pollen tubes, were noted in only one year. Cross- and self-pollination variant showed the existence of specific behaviour of growing pollen tubes in all regions of the ovule, including the embryo sac (except in self-pollination). Only in cross-pollination variant, correlation-regression analysis has shown that specific behaviour of growing pollen tubes did affect the pollen tube penetrating into the nucellus.",
journal = "Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo",
title = "Occurrence of specific behaviour of growing pollen tubes in the ovary of the 'Pozna Plava' plum cultivar",
pages = "12-7",
volume = "1308",
doi = "10.17660/ActaHortic.2021.1308.2"
}

Đorđevic, M., Cerović, R., Radicevic, S., Milosevic, N., Glisic, I. S., Maric, S.,& Lukic, M.. (2021). Occurrence of specific behaviour of growing pollen tubes in the ovary of the 'Pozna Plava' plum cultivar. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308, 7-12.
https://doi.org/10.17660/ActaHortic.2021.1308.2

Đorđevic M, Cerović R, Radicevic S, Milosevic N, Glisic IS, Maric S, Lukic M. Occurrence of specific behaviour of growing pollen tubes in the ovary of the 'Pozna Plava' plum cultivar. in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo. 2021;1308:7-12.
doi:10.17660/ActaHortic.2021.1308.2 .

Đorđevic, Milena, Cerović, Radosav, Radicevic, S., Milosevic, N., Glisic, I. S., Maric, S., Lukic, M., "Occurrence of specific behaviour of growing pollen tubes in the ovary of the 'Pozna Plava' plum cultivar" in Proceedings of the II International Symposium on Fruit Culture Along Silk Road Countries - Fruits Fo, 1308 (2021):7-12,
https://doi.org/10.17660/ActaHortic.2021.1308.2 . .

TY  - JOUR
AU  - Fotirić-Aksić, Milica
AU  - Cerović, Radosav
AU  - Radosevic, R.
AU  - Oparnica, C.
AU  - Meland, M.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4806
AB  - The objective of this study was to determine morphological and anatomical leaf characteristics and stomatal traits of three European (Pyrus communis L.) cultivars, three Asian [Pyrus pyrifolia (Burm. f.) Nakai] pear cultivars together with one interspecies hybrid (P. pyrifolia x P. communis 'Bartlett') and link them with the resistance to pathogens. Pear trees were grown under the standard practice without irrigation. Fully developed leaves were picked from the middle part of the extension shoots at the beginning of the July. Leaf traits were measured on leaves picked the same day. Anatomy of leaves was determined under light microscopy (LM) while scanning electron microscopy (SEM) was used for the examination of the stomata cells. Asian pear cultivars ('Kousui', 'Nijisseiki' and 'Niitaka') had much higher leaf parameters (width, length, stem length and leaf area) than the European cultivars ('Conference', 'Williams Bartlett', 'Abbate Fetel') and interspecies hybrid ('Kieffer Seedling'). Midrib parameters (length and width) were the highest in 'Kosui' and 'Nijisseiki'. Leaves of 'Kieffer Seedling' and 'Abbate Fetel' were the thickest, mainly due to increased palisade and spongy parenchyma thickness. The leaf stomata density significantly varied among the pear cultivars, ranging from 89.53 stomata mm(-2) ('Nijisseiki') up to 134.07 stomata mm(-2) ('Housui'). SEM proved that Asian pear cultivars and 'Kieffer Seedling' shared 'paracytic' stomata type, while European pear cultivars had 'anomocytic' stomata type. Cluster analysis distinguished pear cultivars into two distinct groups, where European cultivars formed first sub-cluster and Asian together with 'Kieffer Seedling' second subcluster. Midrib traits and stomata type made a clear separation between the clusters. These results might suggest that thickness of midrib could be a huge barrier for Psylla sp. probing in Asian pears, thus representing one of the key factors in the resistance of these cultivars.
T2  - XIII International Pear Symposium
T1  - Morphological and anatomical leaf characteristics of some European and Asian pear cultivars
EP  - 70
SP  - 63
VL  - 1303
DO  - 10.17660/ActaHortic.2021.1303.10
ER  - 

@article{
author = "Fotirić-Aksić, Milica and Cerović, Radosav and Radosevic, R. and Oparnica, C. and Meland, M.",
year = "2021",
abstract = "The objective of this study was to determine morphological and anatomical leaf characteristics and stomatal traits of three European (Pyrus communis L.) cultivars, three Asian [Pyrus pyrifolia (Burm. f.) Nakai] pear cultivars together with one interspecies hybrid (P. pyrifolia x P. communis 'Bartlett') and link them with the resistance to pathogens. Pear trees were grown under the standard practice without irrigation. Fully developed leaves were picked from the middle part of the extension shoots at the beginning of the July. Leaf traits were measured on leaves picked the same day. Anatomy of leaves was determined under light microscopy (LM) while scanning electron microscopy (SEM) was used for the examination of the stomata cells. Asian pear cultivars ('Kousui', 'Nijisseiki' and 'Niitaka') had much higher leaf parameters (width, length, stem length and leaf area) than the European cultivars ('Conference', 'Williams Bartlett', 'Abbate Fetel') and interspecies hybrid ('Kieffer Seedling'). Midrib parameters (length and width) were the highest in 'Kosui' and 'Nijisseiki'. Leaves of 'Kieffer Seedling' and 'Abbate Fetel' were the thickest, mainly due to increased palisade and spongy parenchyma thickness. The leaf stomata density significantly varied among the pear cultivars, ranging from 89.53 stomata mm(-2) ('Nijisseiki') up to 134.07 stomata mm(-2) ('Housui'). SEM proved that Asian pear cultivars and 'Kieffer Seedling' shared 'paracytic' stomata type, while European pear cultivars had 'anomocytic' stomata type. Cluster analysis distinguished pear cultivars into two distinct groups, where European cultivars formed first sub-cluster and Asian together with 'Kieffer Seedling' second subcluster. Midrib traits and stomata type made a clear separation between the clusters. These results might suggest that thickness of midrib could be a huge barrier for Psylla sp. probing in Asian pears, thus representing one of the key factors in the resistance of these cultivars.",
journal = "XIII International Pear Symposium",
title = "Morphological and anatomical leaf characteristics of some European and Asian pear cultivars",
pages = "70-63",
volume = "1303",
doi = "10.17660/ActaHortic.2021.1303.10"
}

Fotirić-Aksić, M., Cerović, R., Radosevic, R., Oparnica, C.,& Meland, M.. (2021). Morphological and anatomical leaf characteristics of some European and Asian pear cultivars. in XIII International Pear Symposium, 1303, 63-70.
https://doi.org/10.17660/ActaHortic.2021.1303.10

Fotirić-Aksić M, Cerović R, Radosevic R, Oparnica C, Meland M. Morphological and anatomical leaf characteristics of some European and Asian pear cultivars. in XIII International Pear Symposium. 2021;1303:63-70.
doi:10.17660/ActaHortic.2021.1303.10 .

Fotirić-Aksić, Milica, Cerović, Radosav, Radosevic, R., Oparnica, C., Meland, M., "Morphological and anatomical leaf characteristics of some European and Asian pear cultivars" in XIII International Pear Symposium, 1303 (2021):63-70,
https://doi.org/10.17660/ActaHortic.2021.1303.10 . .

TY  - JOUR
AU  - Meland, Mekjell
AU  - Fotirić-Aksić, Milica
AU  - Cerović, Radosav
AU  - Froynes, Oddmund
AU  - Kaiser, Clive
AU  - Maas, Frank
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4956
AB  - Norwegian pear (Pyrus communis L.) production has been in decline for the last 25 years. This was mainly because of old cultivars, with low yields and poor consumer appeal, could not compete against strong competition from imported pears, mainly 'Conference'. Since 1994, the Norwegian breeding company, Graminor Ltd., has released several new pear cultivars, which have been evaluated at NIBIO Ullensvang, western Norway. The first trial was planted in 1999 and included the Graminor Ltd. cultivars: 'Ingeborg', 'Fritjof' and 'Anna', which were bred by the Norwegian University of Life Sciences. In 2002, a second trial was planted including Graminor Ltd. cultivars: 'Kristina', 'Ingrid' and 'Celina' and these were compared against a control, 'Clara Frijs'. All scion cultivars were grafted on the semi-vigorous rootstock `Brokmal' and grown for 8 years. In both trials, full bloom (similar to 80% of flowers open) took place between 7 May +/- 3 days and 15 May +/- 4 days. Flowering was however, completed within a 7-day period in any one year, ensuring adequate overlap in flowering time between all cultivars. Over the final 4-year period all cultivars were evaluated, 'Anna', 'Fritjof', 'Kristina' and 'Ingeborg' all had moderate cumulative yields (64.7, 66.2, 36.1, and 30.4 kg.tree(-1), respectively). Fruit weight (212 to 183g) and quality of all these cultivars was acceptable (11.2%   lt = TSS   lt = 11.8%; 0.16%   lt = acidity   lt = 0.22%). However, 'Fritjof' had many misshapen fruit and exhibited pre-harvest shriveling in several instances making it unacceptable for commercial plantings. 'Clara Frijs' and 'Celina' cumulative yields were low (12.5 and 21.2 kg per tree, respectively) and fruit were also small (172 to 161 g, respectively). However, due to the attractive cerise-blush 'Celina' (trademarked QTee (R)) pear is now widely planted in Norway and abroad and grafted on Quince rootstocks.
T2  - Journal of the American Pomological Society
T1  - Evaluation of New Promising Norwegian Pear Cultivars in a Nordic Climate
EP  - 156
IS  - 3
SP  - 149
VL  - 75
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4956
ER  - 

@article{
author = "Meland, Mekjell and Fotirić-Aksić, Milica and Cerović, Radosav and Froynes, Oddmund and Kaiser, Clive and Maas, Frank",
year = "2021",
abstract = "Norwegian pear (Pyrus communis L.) production has been in decline for the last 25 years. This was mainly because of old cultivars, with low yields and poor consumer appeal, could not compete against strong competition from imported pears, mainly 'Conference'. Since 1994, the Norwegian breeding company, Graminor Ltd., has released several new pear cultivars, which have been evaluated at NIBIO Ullensvang, western Norway. The first trial was planted in 1999 and included the Graminor Ltd. cultivars: 'Ingeborg', 'Fritjof' and 'Anna', which were bred by the Norwegian University of Life Sciences. In 2002, a second trial was planted including Graminor Ltd. cultivars: 'Kristina', 'Ingrid' and 'Celina' and these were compared against a control, 'Clara Frijs'. All scion cultivars were grafted on the semi-vigorous rootstock `Brokmal' and grown for 8 years. In both trials, full bloom (similar to 80% of flowers open) took place between 7 May +/- 3 days and 15 May +/- 4 days. Flowering was however, completed within a 7-day period in any one year, ensuring adequate overlap in flowering time between all cultivars. Over the final 4-year period all cultivars were evaluated, 'Anna', 'Fritjof', 'Kristina' and 'Ingeborg' all had moderate cumulative yields (64.7, 66.2, 36.1, and 30.4 kg.tree(-1), respectively). Fruit weight (212 to 183g) and quality of all these cultivars was acceptable (11.2%   lt = TSS   lt = 11.8%; 0.16%   lt = acidity   lt = 0.22%). However, 'Fritjof' had many misshapen fruit and exhibited pre-harvest shriveling in several instances making it unacceptable for commercial plantings. 'Clara Frijs' and 'Celina' cumulative yields were low (12.5 and 21.2 kg per tree, respectively) and fruit were also small (172 to 161 g, respectively). However, due to the attractive cerise-blush 'Celina' (trademarked QTee (R)) pear is now widely planted in Norway and abroad and grafted on Quince rootstocks.",
journal = "Journal of the American Pomological Society",
title = "Evaluation of New Promising Norwegian Pear Cultivars in a Nordic Climate",
pages = "156-149",
number = "3",
volume = "75",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4956"
}

Meland, M., Fotirić-Aksić, M., Cerović, R., Froynes, O., Kaiser, C.,& Maas, F.. (2021). Evaluation of New Promising Norwegian Pear Cultivars in a Nordic Climate. in Journal of the American Pomological Society, 75(3), 149-156.
https://hdl.handle.net/21.15107/rcub_technorep_4956

Meland M, Fotirić-Aksić M, Cerović R, Froynes O, Kaiser C, Maas F. Evaluation of New Promising Norwegian Pear Cultivars in a Nordic Climate. in Journal of the American Pomological Society. 2021;75(3):149-156.
https://hdl.handle.net/21.15107/rcub_technorep_4956 .

Meland, Mekjell, Fotirić-Aksić, Milica, Cerović, Radosav, Froynes, Oddmund, Kaiser, Clive, Maas, Frank, "Evaluation of New Promising Norwegian Pear Cultivars in a Nordic Climate" in Journal of the American Pomological Society, 75, no. 3 (2021):149-156,
https://hdl.handle.net/21.15107/rcub_technorep_4956 .

TY  - JOUR
AU  - Cerović, Radosav
AU  - Fotirić-Aksić, Milica
AU  - Đorđevic, Milena
AU  - Meland, Mekjell
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4871
AB  - Norwegian plum production is characterized by climatic limitations, different flowering time, deficiently of wholly-adapted cultivars and appropriate pollen donors for cultivars that can be grown in this region. This study evaluated the progamic phase of fertilization and fruit set in four European plum cultivars (Mallard, Edda, Jubileum, and Reeves) after crossing with different pollinizers over two years (2018/2019). Reproductive parameters, in vitro pollen germination, number of pollen tubes in the upper part of the style and locule of the ovary, number of pistils with ovule penetrated by pollen tube, fruit set in all crossing combinations, and fruit set in open pollination of pollen recipient cultivars showed different adaptability of both recipient and donor cultivars to the specific ecological conditions prevailing in Western Norway. The pollinizers Victoria, Opal and C. Lepotica proved to be a very good pollinizers for cultivar Jubileum, while pollinizers R. C. Souffriau and Valor for the cultivar Reeves. Cultivars Opal, R. E. Prolific and Mallard are excellent pollinizers for Edda in conditions of higher temperatures during flowering period and post-flowering period. Cultivar. C. Lepotica proved to be the best pollinizer for Edda in conditions when the temperatures were lower. Cultivars Opal and R. E. Prolific can be considered as good pollinizers for Mallard
T2  - Scientia Horticulturae
T1  - The effects of pollinizers on pollen tube growth and fruit set of European plum (Prunus domestica L.) in a Nordic climate
VL  - 288
DO  - 10.1016/j.scienta.2021.110390
ER  - 

@article{
author = "Cerović, Radosav and Fotirić-Aksić, Milica and Đorđevic, Milena and Meland, Mekjell",
year = "2021",
abstract = "Norwegian plum production is characterized by climatic limitations, different flowering time, deficiently of wholly-adapted cultivars and appropriate pollen donors for cultivars that can be grown in this region. This study evaluated the progamic phase of fertilization and fruit set in four European plum cultivars (Mallard, Edda, Jubileum, and Reeves) after crossing with different pollinizers over two years (2018/2019). Reproductive parameters, in vitro pollen germination, number of pollen tubes in the upper part of the style and locule of the ovary, number of pistils with ovule penetrated by pollen tube, fruit set in all crossing combinations, and fruit set in open pollination of pollen recipient cultivars showed different adaptability of both recipient and donor cultivars to the specific ecological conditions prevailing in Western Norway. The pollinizers Victoria, Opal and C. Lepotica proved to be a very good pollinizers for cultivar Jubileum, while pollinizers R. C. Souffriau and Valor for the cultivar Reeves. Cultivars Opal, R. E. Prolific and Mallard are excellent pollinizers for Edda in conditions of higher temperatures during flowering period and post-flowering period. Cultivar. C. Lepotica proved to be the best pollinizer for Edda in conditions when the temperatures were lower. Cultivars Opal and R. E. Prolific can be considered as good pollinizers for Mallard",
journal = "Scientia Horticulturae",
title = "The effects of pollinizers on pollen tube growth and fruit set of European plum (Prunus domestica L.) in a Nordic climate",
volume = "288",
doi = "10.1016/j.scienta.2021.110390"
}

Cerović, R., Fotirić-Aksić, M., Đorđevic, M.,& Meland, M.. (2021). The effects of pollinizers on pollen tube growth and fruit set of European plum (Prunus domestica L.) in a Nordic climate. in Scientia Horticulturae, 288.
https://doi.org/10.1016/j.scienta.2021.110390

Cerović R, Fotirić-Aksić M, Đorđevic M, Meland M. The effects of pollinizers on pollen tube growth and fruit set of European plum (Prunus domestica L.) in a Nordic climate. in Scientia Horticulturae. 2021;288.
doi:10.1016/j.scienta.2021.110390 .

Cerović, Radosav, Fotirić-Aksić, Milica, Đorđevic, Milena, Meland, Mekjell, "The effects of pollinizers on pollen tube growth and fruit set of European plum (Prunus domestica L.) in a Nordic climate" in Scientia Horticulturae, 288 (2021),
https://doi.org/10.1016/j.scienta.2021.110390 . .

TY  - JOUR
AU  - Kolarevic, Tijana
AU  - Milincic, Danijel D.
AU  - Vujovic, Tatjana
AU  - Gasic, Uros M.
AU  - Prokic, Ljiljana
AU  - Kostic, Aleksandar Z.
AU  - Cerović, Radosav
AU  - Stanojevic, Sladjana P.
AU  - Tesic, Zivoslav Lj.
AU  - Pesic, Mirjana B.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4814
AB  - The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry 'Cacanska Bestrna' and blueberry 'Toro'. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA(3) ('Cacanska Bestrna') or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA ('Toro'). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D ('Cacanska Bestrna') or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA ('Toro'). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS(& BULL;+) and DPPH & BULL; scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS(& BULL;+) and ten to seventeen times lower DPPH & BULL; scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
T2  - Horticulturae
T1  - Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
IS  - 11
VL  - 7
DO  - 10.3390/horticulturae7110420
ER  - 

@article{
author = "Kolarevic, Tijana and Milincic, Danijel D. and Vujovic, Tatjana and Gasic, Uros M. and Prokic, Ljiljana and Kostic, Aleksandar Z. and Cerović, Radosav and Stanojevic, Sladjana P. and Tesic, Zivoslav Lj. and Pesic, Mirjana B.",
year = "2021",
abstract = "The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry 'Cacanska Bestrna' and blueberry 'Toro'. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA(3) ('Cacanska Bestrna') or on MS medium with macroelements reduced to 1/2, 2 mg/L zeatin and 0.2 mg/L IAA ('Toro'). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D ('Cacanska Bestrna') or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA ('Toro'). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS(& BULL;+) and DPPH & BULL; scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS(& BULL;+) and ten to seventeen times lower DPPH & BULL; scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.",
journal = "Horticulturae",
title = "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry",
number = "11",
volume = "7",
doi = "10.3390/horticulturae7110420"
}

Kolarevic, T., Milincic, D. D., Vujovic, T., Gasic, U. M., Prokic, L., Kostic, A. Z., Cerović, R., Stanojevic, S. P., Tesic, Z. Lj.,& Pesic, M. B.. (2021). Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae, 7(11).
https://doi.org/10.3390/horticulturae7110420

Kolarevic T, Milincic DD, Vujovic T, Gasic UM, Prokic L, Kostic AZ, Cerović R, Stanojevic SP, Tesic ZL, Pesic MB. Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae. 2021;7(11).
doi:10.3390/horticulturae7110420 .

Kolarevic, Tijana, Milincic, Danijel D., Vujovic, Tatjana, Gasic, Uros M., Prokic, Ljiljana, Kostic, Aleksandar Z., Cerović, Radosav, Stanojevic, Sladjana P., Tesic, Zivoslav Lj., Pesic, Mirjana B., "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry" in Horticulturae, 7, no. 11 (2021),
https://doi.org/10.3390/horticulturae7110420 . .

TY  - JOUR
AU  - Radovic, A.
AU  - Nikolic, D.
AU  - Cerović, Radosav
AU  - Milatovic, D.
AU  - Rakonjac, V
AU  - Bakic, I
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4765
AB  - The effect of three different temperatures (8, 16 and 24 degrees C) on pollen germination and pollen tube growth in vitro in eight quince cultivars ('Leskovacka', Vranjska', 'Morava', 'Pazardzijska', 'Hemus', 'Asenica', 'Portugal' and 'Triumph') was studied. The research was carried out in a two-year period (2015-2016). Germination rate and pollen tube growth were determined on the culture medium containing 15% sucrose and 0.7% agar. The temperature showed a significant effect on in vitro pollen germination and pollen tube length. The lowest pollen germination was determined at the temperature of 8 degrees C (22.73%). However, pollen germination was three times higher at temperatures of 16 and 24 degrees C (62.60 and 72.16%, respectively). The temperature effect was significantly more pronounced on the pollen tube length. The smallest pollen tube length was determined at 8 degrees C (241.5 mu m) and it was four to five times lower than the pollen tube length measured at 16 and 24 degrees C (809.6 and 1137.4 mu m, respectively). On this basis, it can be concluded that the temperature of 8 degrees C was not sufficient for pollen germination and pollen tube growth in quince cultivars. However, temperatures of 16 and 24 degrees C were optimal for pollen germination and pollen tube growth.
T2  - IV Balkan Symposium on Fruit Growing
T1  - The effect of temperature on pollen germination and pollen tube growth of quince cultivars
EP  - 72
SP  - 67
VL  - 1289
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4765
ER  - 

@article{
author = "Radovic, A. and Nikolic, D. and Cerović, Radosav and Milatovic, D. and Rakonjac, V and Bakic, I",
year = "2020",
abstract = "The effect of three different temperatures (8, 16 and 24 degrees C) on pollen germination and pollen tube growth in vitro in eight quince cultivars ('Leskovacka', Vranjska', 'Morava', 'Pazardzijska', 'Hemus', 'Asenica', 'Portugal' and 'Triumph') was studied. The research was carried out in a two-year period (2015-2016). Germination rate and pollen tube growth were determined on the culture medium containing 15% sucrose and 0.7% agar. The temperature showed a significant effect on in vitro pollen germination and pollen tube length. The lowest pollen germination was determined at the temperature of 8 degrees C (22.73%). However, pollen germination was three times higher at temperatures of 16 and 24 degrees C (62.60 and 72.16%, respectively). The temperature effect was significantly more pronounced on the pollen tube length. The smallest pollen tube length was determined at 8 degrees C (241.5 mu m) and it was four to five times lower than the pollen tube length measured at 16 and 24 degrees C (809.6 and 1137.4 mu m, respectively). On this basis, it can be concluded that the temperature of 8 degrees C was not sufficient for pollen germination and pollen tube growth in quince cultivars. However, temperatures of 16 and 24 degrees C were optimal for pollen germination and pollen tube growth.",
journal = "IV Balkan Symposium on Fruit Growing",
title = "The effect of temperature on pollen germination and pollen tube growth of quince cultivars",
pages = "72-67",
volume = "1289",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4765"
}

Radovic, A., Nikolic, D., Cerović, R., Milatovic, D., Rakonjac, V.,& Bakic, I.. (2020). The effect of temperature on pollen germination and pollen tube growth of quince cultivars. in IV Balkan Symposium on Fruit Growing, 1289, 67-72.
https://hdl.handle.net/21.15107/rcub_technorep_4765

Radovic A, Nikolic D, Cerović R, Milatovic D, Rakonjac V, Bakic I. The effect of temperature on pollen germination and pollen tube growth of quince cultivars. in IV Balkan Symposium on Fruit Growing. 2020;1289:67-72.
https://hdl.handle.net/21.15107/rcub_technorep_4765 .

Radovic, A., Nikolic, D., Cerović, Radosav, Milatovic, D., Rakonjac, V, Bakic, I, "The effect of temperature on pollen germination and pollen tube growth of quince cultivars" in IV Balkan Symposium on Fruit Growing, 1289 (2020):67-72,
https://hdl.handle.net/21.15107/rcub_technorep_4765 .

TY  - JOUR
AU  - Glisic, I. S.
AU  - Milatovic, D.
AU  - Cerović, Radosav
AU  - Radicevic, S.
AU  - Đorđevic, Milena
AU  - Milosevic, N.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4764
AB  - One of the most important plum (Prunus domestica L.) breeding objectives is regular and large yield which depends on the degree of self-fertility. Partially self-fertile and self-sterile genotypes require compatible pollenizers. The aim of this study was to investigate suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for six promising plum genotypes (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87, and 22/17/87, and the cultivar 'Nada') developed at Fruit Research Institute (FRI), Cacak. Previous studies showed that the abovementioned plum genotypes were characterized by different levels of self-fertility. Also, a satisfactory overlapping in the flowering time between the pollenizer and studied genotypes was found. The investigation was conducted over two years and included pollen germination in vitro of the pollenizer cultivar, as well as quantitative parameters of pollen tube growth in vivo (the number of pollen tubes in the upper third and in the base of the style, number of pollen tubes in the ovary, the percentage of pistils with the pollen tube penetrating the nucellus of the ovule 10 days after pollination) and fruit set under cross-pollination. The same quantitative parameters of pollen tube growth in vivo and fruit set under open pollination were used for comparison. The tested cultivars had good pollen germination, ranging from 44.16% in the first year to 44.57% in the second year of study. All studied plum genotypes except hybrid 22/17/87 had higher values of quantitative parameters of pollen tube growth in vivo and fruit set in the cross-pollination variant compared to the open pollination variant. Average fruit set in the cross-pollination variant ranged from 15.42% (hybrid IV/63/81) to 32.61% ('Nada'). Based on the obtained results, the cultivar 'Cacanska Lepotica' represents a satisfactory pollenizer for hybrids IV/63/81 and 32/21/87, and a good pollenizer for hybrids 38/62/70, 34/41/87, 22/17/87, and the cultivar 'Nada'.
T2  - IV Balkan Symposium on Fruit Growing
T1  - Examination of suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Cacak (Serbia)
EP  - 219
SP  - 213
VL  - 1289
DO  - 10.17660/ActaHortic.2020.1289.30
ER  - 

@article{
author = "Glisic, I. S. and Milatovic, D. and Cerović, Radosav and Radicevic, S. and Đorđevic, Milena and Milosevic, N.",
year = "2020",
abstract = "One of the most important plum (Prunus domestica L.) breeding objectives is regular and large yield which depends on the degree of self-fertility. Partially self-fertile and self-sterile genotypes require compatible pollenizers. The aim of this study was to investigate suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for six promising plum genotypes (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87, and 22/17/87, and the cultivar 'Nada') developed at Fruit Research Institute (FRI), Cacak. Previous studies showed that the abovementioned plum genotypes were characterized by different levels of self-fertility. Also, a satisfactory overlapping in the flowering time between the pollenizer and studied genotypes was found. The investigation was conducted over two years and included pollen germination in vitro of the pollenizer cultivar, as well as quantitative parameters of pollen tube growth in vivo (the number of pollen tubes in the upper third and in the base of the style, number of pollen tubes in the ovary, the percentage of pistils with the pollen tube penetrating the nucellus of the ovule 10 days after pollination) and fruit set under cross-pollination. The same quantitative parameters of pollen tube growth in vivo and fruit set under open pollination were used for comparison. The tested cultivars had good pollen germination, ranging from 44.16% in the first year to 44.57% in the second year of study. All studied plum genotypes except hybrid 22/17/87 had higher values of quantitative parameters of pollen tube growth in vivo and fruit set in the cross-pollination variant compared to the open pollination variant. Average fruit set in the cross-pollination variant ranged from 15.42% (hybrid IV/63/81) to 32.61% ('Nada'). Based on the obtained results, the cultivar 'Cacanska Lepotica' represents a satisfactory pollenizer for hybrids IV/63/81 and 32/21/87, and a good pollenizer for hybrids 38/62/70, 34/41/87, 22/17/87, and the cultivar 'Nada'.",
journal = "IV Balkan Symposium on Fruit Growing",
title = "Examination of suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Cacak (Serbia)",
pages = "219-213",
volume = "1289",
doi = "10.17660/ActaHortic.2020.1289.30"
}

Glisic, I. S., Milatovic, D., Cerović, R., Radicevic, S., Đorđevic, M.,& Milosevic, N.. (2020). Examination of suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Cacak (Serbia). in IV Balkan Symposium on Fruit Growing, 1289, 213-219.
https://doi.org/10.17660/ActaHortic.2020.1289.30

Glisic IS, Milatovic D, Cerović R, Radicevic S, Đorđevic M, Milosevic N. Examination of suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Cacak (Serbia). in IV Balkan Symposium on Fruit Growing. 2020;1289:213-219.
doi:10.17660/ActaHortic.2020.1289.30 .

Glisic, I. S., Milatovic, D., Cerović, Radosav, Radicevic, S., Đorđevic, Milena, Milosevic, N., "Examination of suitability of the cultivar 'Cacanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Cacak (Serbia)" in IV Balkan Symposium on Fruit Growing, 1289 (2020):213-219,
https://doi.org/10.17660/ActaHortic.2020.1289.30 . .

TY  - JOUR
AU  - Cerović, Radosav
AU  - Fotirić-Aksić, Milica
AU  - Meland, Mekjell
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4378
AB  - Norwegian pear production is low due to climatic limitations, a lack of well-adapted cultivars and suitable pollinizers. However, nowadays it is increasing as a result of newly introduced and bred pear cultivars. Since cross pollination is necessary for high yields and good fruit quality, the aim of this investigation was to find the most suitable pollinizers for the pear cultivars "Ingeborg" ("Conference" x "Bonne Louise") and "Celina" ("Coloree de Juillet" x "Williams"). Self-pollination of "Ingeborg" and "Celina", together with "Conference", "Belle Lucrative", "Anna", "Clara Frijs", "Herzogin Elsa", "Kristina" and "Fritjof" as potential pollinizers, were studied in this experiment during the 2017 and 2018 seasons in Norway. The success rate of each pollinizer was tested under field conditions, while the monitoring of pollen tube growth was done using the fluorescence microscopy method. All reproductive parameters (pollen germination, number of pollen tubes in the upper part of the style, pollen tube number in the locule of the ovary, number of fertilized ovules, initial fruit set, and final fruit set) in all crossing combinations were higher in 2018 due to much warmer weather. Based on the flowering overlap and success rate of each individual pollinizer and fruit set, the cultivars "Anna" and "Clara Frijs" can be suggested as pollinizers for the cultivar "Ingeborg", while "Fritjof", "Anna", "Kristina" and "Herzogin Elsa" for the cultivar "Celina". An even distribution of two compatible pollinizers having overlapping flowering times with the main commercial pear cultivar is a general recommendation for commercial pear production.
PB  - MDPI, Basel
T2  - Agronomy-Basel
T1  - Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate
IS  - 7
VL  - 10
DO  - 10.3390/agronomy10070970
ER  - 

@article{
author = "Cerović, Radosav and Fotirić-Aksić, Milica and Meland, Mekjell",
year = "2020",
abstract = "Norwegian pear production is low due to climatic limitations, a lack of well-adapted cultivars and suitable pollinizers. However, nowadays it is increasing as a result of newly introduced and bred pear cultivars. Since cross pollination is necessary for high yields and good fruit quality, the aim of this investigation was to find the most suitable pollinizers for the pear cultivars "Ingeborg" ("Conference" x "Bonne Louise") and "Celina" ("Coloree de Juillet" x "Williams"). Self-pollination of "Ingeborg" and "Celina", together with "Conference", "Belle Lucrative", "Anna", "Clara Frijs", "Herzogin Elsa", "Kristina" and "Fritjof" as potential pollinizers, were studied in this experiment during the 2017 and 2018 seasons in Norway. The success rate of each pollinizer was tested under field conditions, while the monitoring of pollen tube growth was done using the fluorescence microscopy method. All reproductive parameters (pollen germination, number of pollen tubes in the upper part of the style, pollen tube number in the locule of the ovary, number of fertilized ovules, initial fruit set, and final fruit set) in all crossing combinations were higher in 2018 due to much warmer weather. Based on the flowering overlap and success rate of each individual pollinizer and fruit set, the cultivars "Anna" and "Clara Frijs" can be suggested as pollinizers for the cultivar "Ingeborg", while "Fritjof", "Anna", "Kristina" and "Herzogin Elsa" for the cultivar "Celina". An even distribution of two compatible pollinizers having overlapping flowering times with the main commercial pear cultivar is a general recommendation for commercial pear production.",
publisher = "MDPI, Basel",
journal = "Agronomy-Basel",
title = "Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate",
number = "7",
volume = "10",
doi = "10.3390/agronomy10070970"
}

Cerović, R., Fotirić-Aksić, M.,& Meland, M.. (2020). Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate. in Agronomy-Basel
MDPI, Basel., 10(7).
https://doi.org/10.3390/agronomy10070970

Cerović R, Fotirić-Aksić M, Meland M. Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate. in Agronomy-Basel. 2020;10(7).
doi:10.3390/agronomy10070970 .

Cerović, Radosav, Fotirić-Aksić, Milica, Meland, Mekjell, "Success Rate of Individual Pollinizers for the Pear Cultivars "Ingeborg" and "Celina" in a Nordic Climate" in Agronomy-Basel, 10, no. 7 (2020),
https://doi.org/10.3390/agronomy10070970 . .

TY  - JOUR
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Nikolić, Dragan
AU  - Radičević, Sanja
AU  - Glišić, Ivana
AU  - Milošević, Nebojša
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4447
AB  - Pollen grain micromorphological characterisation of four plum (Prunus domestica L.) genotypes from two different breeding programmes (Fruit Research Institute, Serbia, and Hohenheim, Germany) was performed using scanning electron microscopy (SEM). The studied characteristics were pollen size and shape, colpus length, mesocolpium width as well as exine characteristics. All genotypes had tricolpate pollen grains and striate exine ornamentation, with more parallel longitudinal ridges. The most significant differences among the analysed genotypes concerned the length of the pollen grain and exine sculpturing. The genotypes Pozna Plava', Presenta' and 'Caanska najbolja' had the largest pollen grains ( gt  57 mu m), whereas `Hanita' showed a smaller pollen size, below 54 mu m. Pozna Plava' had perprolate pollen in the equatorial view (LAW ratio 2.06), while the other three genotypes had prolate shaped pollen. By analysing the pollen morphological characteristics of the plum varieties in this paper, primarily the shape, size, length of colpi and, most importantly, the sculpture of the exine, each variety showed a specificity which can be used together with other morphological characteristics for description and characterisation.
PB  - Publ House Bulgarian Acad Sci, Sofia
T2  - Comptes Rendus de l Academie Bulgare Des Sciences
T1  - Using scanning electron microscopy to characterise plum (prunus domestica l.) Genotypes
EP  - 1397
IS  - 10
SP  - 1390
VL  - 73
DO  - 10.7546/CRABS.2020.10.08
ER  - 

@article{
author = "Đorđević, Milena and Cerović, Radosav and Nikolić, Dragan and Radičević, Sanja and Glišić, Ivana and Milošević, Nebojša",
year = "2020",
abstract = "Pollen grain micromorphological characterisation of four plum (Prunus domestica L.) genotypes from two different breeding programmes (Fruit Research Institute, Serbia, and Hohenheim, Germany) was performed using scanning electron microscopy (SEM). The studied characteristics were pollen size and shape, colpus length, mesocolpium width as well as exine characteristics. All genotypes had tricolpate pollen grains and striate exine ornamentation, with more parallel longitudinal ridges. The most significant differences among the analysed genotypes concerned the length of the pollen grain and exine sculpturing. The genotypes Pozna Plava', Presenta' and 'Caanska najbolja' had the largest pollen grains ( gt  57 mu m), whereas `Hanita' showed a smaller pollen size, below 54 mu m. Pozna Plava' had perprolate pollen in the equatorial view (LAW ratio 2.06), while the other three genotypes had prolate shaped pollen. By analysing the pollen morphological characteristics of the plum varieties in this paper, primarily the shape, size, length of colpi and, most importantly, the sculpture of the exine, each variety showed a specificity which can be used together with other morphological characteristics for description and characterisation.",
publisher = "Publ House Bulgarian Acad Sci, Sofia",
journal = "Comptes Rendus de l Academie Bulgare Des Sciences",
title = "Using scanning electron microscopy to characterise plum (prunus domestica l.) Genotypes",
pages = "1397-1390",
number = "10",
volume = "73",
doi = "10.7546/CRABS.2020.10.08"
}

Đorđević, M., Cerović, R., Nikolić, D., Radičević, S., Glišić, I.,& Milošević, N.. (2020). Using scanning electron microscopy to characterise plum (prunus domestica l.) Genotypes. in Comptes Rendus de l Academie Bulgare Des Sciences
Publ House Bulgarian Acad Sci, Sofia., 73(10), 1390-1397.
https://doi.org/10.7546/CRABS.2020.10.08

Đorđević M, Cerović R, Nikolić D, Radičević S, Glišić I, Milošević N. Using scanning electron microscopy to characterise plum (prunus domestica l.) Genotypes. in Comptes Rendus de l Academie Bulgare Des Sciences. 2020;73(10):1390-1397.
doi:10.7546/CRABS.2020.10.08 .

Đorđević, Milena, Cerović, Radosav, Nikolić, Dragan, Radičević, Sanja, Glišić, Ivana, Milošević, Nebojša, "Using scanning electron microscopy to characterise plum (prunus domestica l.) Genotypes" in Comptes Rendus de l Academie Bulgare Des Sciences, 73, no. 10 (2020):1390-1397,
https://doi.org/10.7546/CRABS.2020.10.08 . .

TY  - JOUR
AU  - Cerović, Radosav
AU  - Akšić, M.F.
AU  - Ðorđević, M.
AU  - Meland, Mekjell
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4426
AB  - Since the European pear (Pyrus communis L.) is a self-incompatible fruit species, synchrony and compatibility between female parts of the mother plant and male gametes from the pollen donor must be fulfilled. Besides pollination and fertilization, normal embryo and zygote development is one of the prerequisites for the satisfactory yields in pears. The main goal of this experiment was to investigate the functionality of embryo sacs and the embryo’s early stages of growth in relation to the fruit set of diploid (‘Celina’) and the triploid (‘Ingeborg’) pear cultivars under specific Norwegian climatic conditions. For this purpose, flowers were collected at the beginning of flowering, and on the third, sixth, ninth, and twelfth days after the beginning of this phenophase for two consecutive years. Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under the light microscope. In the analyzed cultivars, results showed different tendencies in embryo sac development and degradation processes, in both experimental years, which is probably due to the genetic background of the examined cultivars. Also, fertilization success and fruit set were higher in the second year of study due to the higher average temperature during the flowering period. Diploid cultivar ‘Celina’ showed much better adaptation to high temperatures in relation to triploid cultivar ‘Ingeborg’.
PB  - MDPI, Basel
T2  - Plants
T1  - Functionality of embryo sacs in pear cultivars ‘ingeborg’ and ‘celina’ as related to fruit set under nordic climate
EP  - 14
IS  - 12
SP  - 1
VL  - 9
DO  - 10.3390/plants9121716
ER  - 

@article{
author = "Cerović, Radosav and Akšić, M.F. and Ðorđević, M. and Meland, Mekjell",
year = "2020",
abstract = "Since the European pear (Pyrus communis L.) is a self-incompatible fruit species, synchrony and compatibility between female parts of the mother plant and male gametes from the pollen donor must be fulfilled. Besides pollination and fertilization, normal embryo and zygote development is one of the prerequisites for the satisfactory yields in pears. The main goal of this experiment was to investigate the functionality of embryo sacs and the embryo’s early stages of growth in relation to the fruit set of diploid (‘Celina’) and the triploid (‘Ingeborg’) pear cultivars under specific Norwegian climatic conditions. For this purpose, flowers were collected at the beginning of flowering, and on the third, sixth, ninth, and twelfth days after the beginning of this phenophase for two consecutive years. Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under the light microscope. In the analyzed cultivars, results showed different tendencies in embryo sac development and degradation processes, in both experimental years, which is probably due to the genetic background of the examined cultivars. Also, fertilization success and fruit set were higher in the second year of study due to the higher average temperature during the flowering period. Diploid cultivar ‘Celina’ showed much better adaptation to high temperatures in relation to triploid cultivar ‘Ingeborg’.",
publisher = "MDPI, Basel",
journal = "Plants",
title = "Functionality of embryo sacs in pear cultivars ‘ingeborg’ and ‘celina’ as related to fruit set under nordic climate",
pages = "14-1",
number = "12",
volume = "9",
doi = "10.3390/plants9121716"
}

Cerović, R., Akšić, M.F., Ðorđević, M.,& Meland, M.. (2020). Functionality of embryo sacs in pear cultivars ‘ingeborg’ and ‘celina’ as related to fruit set under nordic climate. in Plants
MDPI, Basel., 9(12), 1-14.
https://doi.org/10.3390/plants9121716

Cerović R, Akšić M, Ðorđević M, Meland M. Functionality of embryo sacs in pear cultivars ‘ingeborg’ and ‘celina’ as related to fruit set under nordic climate. in Plants. 2020;9(12):1-14.
doi:10.3390/plants9121716 .

Cerović, Radosav, Akšić, M.F., Ðorđević, M., Meland, Mekjell, "Functionality of embryo sacs in pear cultivars ‘ingeborg’ and ‘celina’ as related to fruit set under nordic climate" in Plants, 9, no. 12 (2020):1-14,
https://doi.org/10.3390/plants9121716 . .

TY  - JOUR
AU  - Radović, Aleksandar
AU  - Cerović, Radosav
AU  - Milatović, Dragan
AU  - Nikolić, Dragan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4390
AB  - Aim of study: To determine the self-compatibility level of eight quince cultivars. Area of study: The region of Belgrade (Central Serbia). Material and methods: Pollen tube growth in vivo and fruit set in two pollination variants (self- and open-pollination) were studied in eight quince cultivars. The quantitative parameters of pollen tube growth (average number of pollen tubes in the upper and middle third of the style, base of the style and in the ovary; the dynamics of pollen tube growth through these parts of the pistil) was determined using the fluorescence microscopy. Main results: The parameters of pollen tube growth and fruit set were primarily dependent on the genotype and variants of pollination. All studied parameters were significantly higher in the open-pollination variant compared with the self-pollination in all cultivars. In the self-pollination variant, 'Leskovacka' and Vranjska' had the highest number of pollen tubes that penetrated the ovary (2.10 and 0.54 in average, respectively), as well as the largest percentage of pistils with the penetration of pollen tubes in the nucellus of ovules six days after pollination (40.09% and 14.74%). Also, they had the highest percentage of initial fruit set (17.01% and 28.52%) and final fruit set (9.32% and 9.86%). Based on this, 'Leskovacka' and 'Vranjska' can be classified as self-compatible cultivars, while the others are self-incompatible. Research highlights: The majority of quince cultivars were self-incompatible. When establishing new orchards with these cultivars, care should be taken about the choice of pollenisers in order to achieve high yields.
PB  - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
T2  - Spanish Journal of Agricultural Research
T1  - Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.)
IS  - 2
VL  - 18
DO  - 10.5424/sjar/2020182-15551
ER  - 

@article{
author = "Radović, Aleksandar and Cerović, Radosav and Milatović, Dragan and Nikolić, Dragan",
year = "2020",
abstract = "Aim of study: To determine the self-compatibility level of eight quince cultivars. Area of study: The region of Belgrade (Central Serbia). Material and methods: Pollen tube growth in vivo and fruit set in two pollination variants (self- and open-pollination) were studied in eight quince cultivars. The quantitative parameters of pollen tube growth (average number of pollen tubes in the upper and middle third of the style, base of the style and in the ovary; the dynamics of pollen tube growth through these parts of the pistil) was determined using the fluorescence microscopy. Main results: The parameters of pollen tube growth and fruit set were primarily dependent on the genotype and variants of pollination. All studied parameters were significantly higher in the open-pollination variant compared with the self-pollination in all cultivars. In the self-pollination variant, 'Leskovacka' and Vranjska' had the highest number of pollen tubes that penetrated the ovary (2.10 and 0.54 in average, respectively), as well as the largest percentage of pistils with the penetration of pollen tubes in the nucellus of ovules six days after pollination (40.09% and 14.74%). Also, they had the highest percentage of initial fruit set (17.01% and 28.52%) and final fruit set (9.32% and 9.86%). Based on this, 'Leskovacka' and 'Vranjska' can be classified as self-compatible cultivars, while the others are self-incompatible. Research highlights: The majority of quince cultivars were self-incompatible. When establishing new orchards with these cultivars, care should be taken about the choice of pollenisers in order to achieve high yields.",
publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid",
journal = "Spanish Journal of Agricultural Research",
title = "Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.)",
number = "2",
volume = "18",
doi = "10.5424/sjar/2020182-15551"
}

Radović, A., Cerović, R., Milatović, D.,& Nikolić, D.. (2020). Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.). in Spanish Journal of Agricultural Research
Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 18(2).
https://doi.org/10.5424/sjar/2020182-15551

Radović A, Cerović R, Milatović D, Nikolić D. Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.). in Spanish Journal of Agricultural Research. 2020;18(2).
doi:10.5424/sjar/2020182-15551 .

Radović, Aleksandar, Cerović, Radosav, Milatović, Dragan, Nikolić, Dragan, "Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.)" in Spanish Journal of Agricultural Research, 18, no. 2 (2020),
https://doi.org/10.5424/sjar/2020182-15551 . .

TY  - JOUR
AU  - Cerović, Radosav
AU  - Fotirić-Aksić, Milica
AU  - Đorđevic, Milena
AU  - Meland, Mekjell
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4738
AB  - Since the European pear (Pyrus communis L.) is a self-incompatible fruit species, synchrony and compatibility between female parts of the mother plant and male gametes from the pollen donor must be fulfilled. Besides pollination and fertilization, normal embryo and zygote development is one of the prerequisites for the satisfactory yields in pears. The main goal of this experiment was to investigate the functionality of embryo sacs and the embryo's early stages of growth in relation to the fruit set of diploid ('Celina') and the triploid ('Ingeborg') pear cultivars under specific Norwegian climatic conditions. For this purpose, flowers were collected at the beginning of flowering, and on the third, sixth, ninth, and twelfth days after the beginning of this phenophase for two consecutive years. Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under the light microscope. In the analyzed cultivars, results showed different tendencies in embryo sac development and degradation processes, in both experimental years, which is probably due to the genetic background of the examined cultivars. Also, fertilization success and fruit set were higher in the second year of study due to the higher average temperature during the flowering period. Diploid cultivar 'Celina' showed much better adaptation to high temperatures in relation to triploid cultivar 'Ingeborg'.
T2  - Plants-Basel
T1  - Functionality of Embryo Sacs in Pear Cultivars 'Ingeborg' and 'Celina' as Related to Fruit Set under Nordic Climate
IS  - 12
VL  - 9
DO  - 10.3390/plants9121716
ER  - 

@article{
author = "Cerović, Radosav and Fotirić-Aksić, Milica and Đorđevic, Milena and Meland, Mekjell",
year = "2020",
abstract = "Since the European pear (Pyrus communis L.) is a self-incompatible fruit species, synchrony and compatibility between female parts of the mother plant and male gametes from the pollen donor must be fulfilled. Besides pollination and fertilization, normal embryo and zygote development is one of the prerequisites for the satisfactory yields in pears. The main goal of this experiment was to investigate the functionality of embryo sacs and the embryo's early stages of growth in relation to the fruit set of diploid ('Celina') and the triploid ('Ingeborg') pear cultivars under specific Norwegian climatic conditions. For this purpose, flowers were collected at the beginning of flowering, and on the third, sixth, ninth, and twelfth days after the beginning of this phenophase for two consecutive years. Ovaries were dehydrated, embedded in paraffin wax, sectioned, stained, and observed under the light microscope. In the analyzed cultivars, results showed different tendencies in embryo sac development and degradation processes, in both experimental years, which is probably due to the genetic background of the examined cultivars. Also, fertilization success and fruit set were higher in the second year of study due to the higher average temperature during the flowering period. Diploid cultivar 'Celina' showed much better adaptation to high temperatures in relation to triploid cultivar 'Ingeborg'.",
journal = "Plants-Basel",
title = "Functionality of Embryo Sacs in Pear Cultivars 'Ingeborg' and 'Celina' as Related to Fruit Set under Nordic Climate",
number = "12",
volume = "9",
doi = "10.3390/plants9121716"
}

Cerović, R., Fotirić-Aksić, M., Đorđevic, M.,& Meland, M.. (2020). Functionality of Embryo Sacs in Pear Cultivars 'Ingeborg' and 'Celina' as Related to Fruit Set under Nordic Climate. in Plants-Basel, 9(12).
https://doi.org/10.3390/plants9121716

Cerović R, Fotirić-Aksić M, Đorđevic M, Meland M. Functionality of Embryo Sacs in Pear Cultivars 'Ingeborg' and 'Celina' as Related to Fruit Set under Nordic Climate. in Plants-Basel. 2020;9(12).
doi:10.3390/plants9121716 .

Cerović, Radosav, Fotirić-Aksić, Milica, Đorđevic, Milena, Meland, Mekjell, "Functionality of Embryo Sacs in Pear Cultivars 'Ingeborg' and 'Celina' as Related to Fruit Set under Nordic Climate" in Plants-Basel, 9, no. 12 (2020),
https://doi.org/10.3390/plants9121716 . .

TY  - JOUR
AU  - Glišić, Ivana
AU  - Milatović, Dragan
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Đorđević, M.
AU  - Milošević, Nebojša
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4578
AB  - One of the most important plum (Prunus domestica L.) breeding objectives is regular and large yield which depends on the degree of self-fertility. Partially self-fertile and self-sterile genotypes require compatible pollenizers. The aim of this study was to investigate suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for six promising plum genotypes (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87, and 22/17/87, and the cultivar 'Nada') developed at Fruit Research Institute (FRI), Čačak. Previous studies showed that the abovementioned plum genotypes were characterized by different levels of self-fertility. Also, a satisfactory overlapping in the flowering time between the pollenizer and studied genotypes was found. The investigation was conducted over two years and included pollen germination in vitro of the pollenizer cultivar, as well as quantitative parameters of pollen tube growth in vivo (the number of pollen tubes in the upper third and in the base of the style, number of pollen tubes in the ovary, the percentage of pistils with the pollen tube penetrating the nucellus of the ovule 10 days after pollination) and fruit set under cross-pollination. The same quantitative parameters of pollen tube growth in vivo and fruit set under open pollination were used for comparison. The tested cultivars had good pollen germination, ranging from 44.16% in the first year to 44.57% in the second year of study. All studied plum genotypes except hybrid 22/17/87 had higher values of quantitative parameters of pollen tube growth in vivo and fruit set in the cross-pollination variant compared to the open pollination variant. Average fruit set in the cross-pollination variant ranged from 15.42% (hybrid IV/63/81) to 32.61% ('Nada'). Based on the obtained results, the cultivar 'Čačanska Lepotica' represents a satisfactory pollenizer for hybrids IV/63/81 and 32/21/87, and a good pollenizer for hybrids 38/62/70, 34/41/87, 22/17/87, and the cultivar 'Nada'.
PB  - Int Soc Horticultural Science, Leuven 1
T2  - Acta Horticulturae
T1  - Examination of suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Čačak (Serbia)
EP  - 219
SP  - 213
VL  - 1289
DO  - 10.17660/ActaHortic.2020.1289.30
ER  - 

@article{
author = "Glišić, Ivana and Milatović, Dragan and Cerović, Radosav and Radičević, Sanja and Đorđević, M. and Milošević, Nebojša",
year = "2020",
abstract = "One of the most important plum (Prunus domestica L.) breeding objectives is regular and large yield which depends on the degree of self-fertility. Partially self-fertile and self-sterile genotypes require compatible pollenizers. The aim of this study was to investigate suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for six promising plum genotypes (hybrids 38/62/70, IV/63/81, 32/21/87, 34/41/87, and 22/17/87, and the cultivar 'Nada') developed at Fruit Research Institute (FRI), Čačak. Previous studies showed that the abovementioned plum genotypes were characterized by different levels of self-fertility. Also, a satisfactory overlapping in the flowering time between the pollenizer and studied genotypes was found. The investigation was conducted over two years and included pollen germination in vitro of the pollenizer cultivar, as well as quantitative parameters of pollen tube growth in vivo (the number of pollen tubes in the upper third and in the base of the style, number of pollen tubes in the ovary, the percentage of pistils with the pollen tube penetrating the nucellus of the ovule 10 days after pollination) and fruit set under cross-pollination. The same quantitative parameters of pollen tube growth in vivo and fruit set under open pollination were used for comparison. The tested cultivars had good pollen germination, ranging from 44.16% in the first year to 44.57% in the second year of study. All studied plum genotypes except hybrid 22/17/87 had higher values of quantitative parameters of pollen tube growth in vivo and fruit set in the cross-pollination variant compared to the open pollination variant. Average fruit set in the cross-pollination variant ranged from 15.42% (hybrid IV/63/81) to 32.61% ('Nada'). Based on the obtained results, the cultivar 'Čačanska Lepotica' represents a satisfactory pollenizer for hybrids IV/63/81 and 32/21/87, and a good pollenizer for hybrids 38/62/70, 34/41/87, 22/17/87, and the cultivar 'Nada'.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "Acta Horticulturae",
title = "Examination of suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Čačak (Serbia)",
pages = "219-213",
volume = "1289",
doi = "10.17660/ActaHortic.2020.1289.30"
}

Glišić, I., Milatović, D., Cerović, R., Radičević, S., Đorđević, M.,& Milošević, N.. (2020). Examination of suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Čačak (Serbia). in Acta Horticulturae
Int Soc Horticultural Science, Leuven 1., 1289, 213-219.
https://doi.org/10.17660/ActaHortic.2020.1289.30

Glišić I, Milatović D, Cerović R, Radičević S, Đorđević M, Milošević N. Examination of suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Čačak (Serbia). in Acta Horticulturae. 2020;1289:213-219.
doi:10.17660/ActaHortic.2020.1289.30 .

Glišić, Ivana, Milatović, Dragan, Cerović, Radosav, Radičević, Sanja, Đorđević, M., Milošević, Nebojša, "Examination of suitability of the cultivar 'Čačanska Lepotica' as a pollenizer for promising plum genotypes developed at FRI, Čačak (Serbia)" in Acta Horticulturae, 1289 (2020):213-219,
https://doi.org/10.17660/ActaHortic.2020.1289.30 . .

TY  - JOUR
AU  - Radović, Aleksandar
AU  - Nikolić, D.
AU  - Cerović, Radosav
AU  - Milatović, Dragan
AU  - Rakonjac, Vera
AU  - Bakić, I.
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4564
AB  - The effect of three different temperatures (8, 16 and 24°C) on pollen germination and pollen tube growth in vitro in eight quince cultivars (‘Leskovacka’, Vranjska’, ‘Morava’, ‘Pazardzijska’, ‘Hemus’, ‘Asenica’, ‘Portugal’ and ‘Triumph’) was studied. The research was carried out in a two-year period (2015-2016). Germination rate and pollen tube growth were determined on the culture medium containing 15% sucrose and 0.7% agar. The temperature showed a significant effect on in vitro pollen germination and pollen tube length. The lowest pollen germination was determined at the temperature of 8°C (22.73%). However, pollen germination was three times higher at temperatures of 16 and 24°C (62.60 and 72.16%, respectively). The temperature effect was significantly more pronounced on the pollen tube length. The smallest pollen tube length was determined at 8°C (241.5 μm) and it was four to five times lower than the pollen tube length measured at 16 and 24°C (809.6 and 1137.4 μm, respectively). On this basis, it can be concluded that the temperature of 8°C was not sufficient for pollen germination and pollen tube growth in quince cultivars. However, temperatures of 16 and 24°C were optimal for pollen germination and pollen tube growth.
PB  - Int Soc Horticultural Science, Leuven 1
T2  - Acta Horticulturae
T1  - The effect of temperature on pollen germination and pollen tube growth of quince cultivars
EP  - 72
SP  - 67
VL  - 1289
DO  - 10.17660/ActaHortic.2020.1289.10
ER  - 

@article{
author = "Radović, Aleksandar and Nikolić, D. and Cerović, Radosav and Milatović, Dragan and Rakonjac, Vera and Bakić, I.",
year = "2020",
abstract = "The effect of three different temperatures (8, 16 and 24°C) on pollen germination and pollen tube growth in vitro in eight quince cultivars (‘Leskovacka’, Vranjska’, ‘Morava’, ‘Pazardzijska’, ‘Hemus’, ‘Asenica’, ‘Portugal’ and ‘Triumph’) was studied. The research was carried out in a two-year period (2015-2016). Germination rate and pollen tube growth were determined on the culture medium containing 15% sucrose and 0.7% agar. The temperature showed a significant effect on in vitro pollen germination and pollen tube length. The lowest pollen germination was determined at the temperature of 8°C (22.73%). However, pollen germination was three times higher at temperatures of 16 and 24°C (62.60 and 72.16%, respectively). The temperature effect was significantly more pronounced on the pollen tube length. The smallest pollen tube length was determined at 8°C (241.5 μm) and it was four to five times lower than the pollen tube length measured at 16 and 24°C (809.6 and 1137.4 μm, respectively). On this basis, it can be concluded that the temperature of 8°C was not sufficient for pollen germination and pollen tube growth in quince cultivars. However, temperatures of 16 and 24°C were optimal for pollen germination and pollen tube growth.",
publisher = "Int Soc Horticultural Science, Leuven 1",
journal = "Acta Horticulturae",
title = "The effect of temperature on pollen germination and pollen tube growth of quince cultivars",
pages = "72-67",
volume = "1289",
doi = "10.17660/ActaHortic.2020.1289.10"
}

Radović, A., Nikolić, D., Cerović, R., Milatović, D., Rakonjac, V.,& Bakić, I.. (2020). The effect of temperature on pollen germination and pollen tube growth of quince cultivars. in Acta Horticulturae
Int Soc Horticultural Science, Leuven 1., 1289, 67-72.
https://doi.org/10.17660/ActaHortic.2020.1289.10

Radović A, Nikolić D, Cerović R, Milatović D, Rakonjac V, Bakić I. The effect of temperature on pollen germination and pollen tube growth of quince cultivars. in Acta Horticulturae. 2020;1289:67-72.
doi:10.17660/ActaHortic.2020.1289.10 .

Radović, Aleksandar, Nikolić, D., Cerović, Radosav, Milatović, Dragan, Rakonjac, Vera, Bakić, I., "The effect of temperature on pollen germination and pollen tube growth of quince cultivars" in Acta Horticulturae, 1289 (2020):67-72,
https://doi.org/10.17660/ActaHortic.2020.1289.10 . .

TY  - JOUR
AU  - Radicevic, S.
AU  - Maric, S.
AU  - Cerović, Radosav
AU  - Milosevic, N.
AU  - Paunovic, S. M.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4734
AB  - Autochthonous cherry genotypes in the Republic of Serbia can potentially provide a rich and useful genetic variability, especially for ripening time, fruit quality and resistance to diseases. Fruit Research Institute, Cacak has a long tradition in collecting new sweet and sour cherry genotypes, as well as in evaluation and utilization of autochthonous material with good agronomic properties. This study was carried out to determine the main biological properties of six sweet cherry (Prunus avium L.) genotypes ('GT-1', 'GT-2', 'GT-3', 'GT-4', 'GT-5', 'GT-6'), and eight sour cherry (Prunus cerasus L.) genotypes ('GV-1', 'GV-2', 'GV-3', 'GV-6', 'GV-7', 'GV-10', 'GV-12', 'GV-13'). Over two years (2016-2017), the following properties of in situ cherry genotypes in West Serbia region were investigated: ripening time, pomological properties (morphometric and chemical), and field resistance to causal agents of cherry diseases - cherry leaf spot (Blumeriella jaapii (Rehm.) v. Arx.), brown rot (Monilinia spp.) and cherry fruit fly (Rhagoletis cerasi L.). The highest average fruit weight among investigated sweet and sour cherry genotypes was found in 'GT-5' (11.00 g) and 'GV-13' (7.64 g), respectively. The highest soluble solids content was found in 'GT-1' and 'GT-3' (16.20%) and 'GV-7' (17.30%), respectively. In terms of field resistance to pathogens, 'GT-1', 'GV-6' and 'GV-10' showed the best performance. To avoid the loss of this material, it is necessary to collect and characterize these genotypes to allow their conservation in germplasm collections, and to provide preconditions for a successful cherry breeding work.
T2  - Iii International Symposium on Horticultural Crop Wild Relatives
T1  - In situ characterization of some sweet and sour cherry autochthonous genotypes in West Serbia region
EP  - 89
SP  - 81
VL  - 1259
DO  - 10.17660/ActaHortic.2019.1259.15
ER  - 

@article{
author = "Radicevic, S. and Maric, S. and Cerović, Radosav and Milosevic, N. and Paunovic, S. M.",
year = "2019",
abstract = "Autochthonous cherry genotypes in the Republic of Serbia can potentially provide a rich and useful genetic variability, especially for ripening time, fruit quality and resistance to diseases. Fruit Research Institute, Cacak has a long tradition in collecting new sweet and sour cherry genotypes, as well as in evaluation and utilization of autochthonous material with good agronomic properties. This study was carried out to determine the main biological properties of six sweet cherry (Prunus avium L.) genotypes ('GT-1', 'GT-2', 'GT-3', 'GT-4', 'GT-5', 'GT-6'), and eight sour cherry (Prunus cerasus L.) genotypes ('GV-1', 'GV-2', 'GV-3', 'GV-6', 'GV-7', 'GV-10', 'GV-12', 'GV-13'). Over two years (2016-2017), the following properties of in situ cherry genotypes in West Serbia region were investigated: ripening time, pomological properties (morphometric and chemical), and field resistance to causal agents of cherry diseases - cherry leaf spot (Blumeriella jaapii (Rehm.) v. Arx.), brown rot (Monilinia spp.) and cherry fruit fly (Rhagoletis cerasi L.). The highest average fruit weight among investigated sweet and sour cherry genotypes was found in 'GT-5' (11.00 g) and 'GV-13' (7.64 g), respectively. The highest soluble solids content was found in 'GT-1' and 'GT-3' (16.20%) and 'GV-7' (17.30%), respectively. In terms of field resistance to pathogens, 'GT-1', 'GV-6' and 'GV-10' showed the best performance. To avoid the loss of this material, it is necessary to collect and characterize these genotypes to allow their conservation in germplasm collections, and to provide preconditions for a successful cherry breeding work.",
journal = "Iii International Symposium on Horticultural Crop Wild Relatives",
title = "In situ characterization of some sweet and sour cherry autochthonous genotypes in West Serbia region",
pages = "89-81",
volume = "1259",
doi = "10.17660/ActaHortic.2019.1259.15"
}

Radicevic, S., Maric, S., Cerović, R., Milosevic, N.,& Paunovic, S. M.. (2019). In situ characterization of some sweet and sour cherry autochthonous genotypes in West Serbia region. in Iii International Symposium on Horticultural Crop Wild Relatives, 1259, 81-89.
https://doi.org/10.17660/ActaHortic.2019.1259.15

Radicevic S, Maric S, Cerović R, Milosevic N, Paunovic SM. In situ characterization of some sweet and sour cherry autochthonous genotypes in West Serbia region. in Iii International Symposium on Horticultural Crop Wild Relatives. 2019;1259:81-89.
doi:10.17660/ActaHortic.2019.1259.15 .

Radicevic, S., Maric, S., Cerović, Radosav, Milosevic, N., Paunovic, S. M., "In situ characterization of some sweet and sour cherry autochthonous genotypes in West Serbia region" in Iii International Symposium on Horticultural Crop Wild Relatives, 1259 (2019):81-89,
https://doi.org/10.17660/ActaHortic.2019.1259.15 . .

TY  - JOUR
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Nikolić, Dragan
AU  - Milošević, Nebojša
AU  - Glišić, Ivana
AU  - Marić, Slađana
AU  - Lukić, Milan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4117
AB  - A newly released, late ripening plum cultivar 'Pozna Plava' sets fruit poorly, although it produces high quality fruit. This study aimed to evaluate which factors in the reproductive process could be related to the lack of fruit set. In two consecutive years, establishment of a suitable polleniser and the stage of ovule development at anthesis as well as initial and final fruit set have been studied. In addition to this, the impact made by temperature fluctuations on the interaction between male gametophytes and female sporophytes was also analysed. Growth of the pollen tubes in the style and penetration into the nucellus as well as fruit set were more effective in cross-pollination than in open and self-pollination. A relative delay in ovule development was observed, and most ovules had an embryo sac with eight nuclei. Considering the results of the quantitative parameter study of pollen tube growth in the ovary as well as the results of the stage of ovule development, a conclusion can be made that this cultivar is characterised by an extremely short effective period of pollination.
PB  - Springer, New York
T2  - Erwerbs-Obstbau
T1  - Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set
EP  - 322
IS  - 4
SP  - 313
VL  - 61
DO  - 10.1007/s10341-019-00431-7
ER  - 

@article{
author = "Đorđević, Milena and Cerović, Radosav and Radičević, Sanja and Nikolić, Dragan and Milošević, Nebojša and Glišić, Ivana and Marić, Slađana and Lukić, Milan",
year = "2019",
abstract = "A newly released, late ripening plum cultivar 'Pozna Plava' sets fruit poorly, although it produces high quality fruit. This study aimed to evaluate which factors in the reproductive process could be related to the lack of fruit set. In two consecutive years, establishment of a suitable polleniser and the stage of ovule development at anthesis as well as initial and final fruit set have been studied. In addition to this, the impact made by temperature fluctuations on the interaction between male gametophytes and female sporophytes was also analysed. Growth of the pollen tubes in the style and penetration into the nucellus as well as fruit set were more effective in cross-pollination than in open and self-pollination. A relative delay in ovule development was observed, and most ovules had an embryo sac with eight nuclei. Considering the results of the quantitative parameter study of pollen tube growth in the ovary as well as the results of the stage of ovule development, a conclusion can be made that this cultivar is characterised by an extremely short effective period of pollination.",
publisher = "Springer, New York",
journal = "Erwerbs-Obstbau",
title = "Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set",
pages = "322-313",
number = "4",
volume = "61",
doi = "10.1007/s10341-019-00431-7"
}

Đorđević, M., Cerović, R., Radičević, S., Nikolić, D., Milošević, N., Glišić, I., Marić, S.,& Lukić, M.. (2019). Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set. in Erwerbs-Obstbau
Springer, New York., 61(4), 313-322.
https://doi.org/10.1007/s10341-019-00431-7

Đorđević M, Cerović R, Radičević S, Nikolić D, Milošević N, Glišić I, Marić S, Lukić M. Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set. in Erwerbs-Obstbau. 2019;61(4):313-322.
doi:10.1007/s10341-019-00431-7 .

Đorđević, Milena, Cerović, Radosav, Radičević, Sanja, Nikolić, Dragan, Milošević, Nebojša, Glišić, Ivana, Marić, Slađana, Lukić, Milan, "Pollen Tube Growth and Embryo Sac Development in 'Pozna Plava' Plum Cultivar Related to Fruit Set" in Erwerbs-Obstbau, 61, no. 4 (2019):313-322,
https://doi.org/10.1007/s10341-019-00431-7 . .

TY  - JOUR
AU  - Đorđević, Milena
AU  - Cerović, Radosav
AU  - Radičević, Sanja
AU  - Glišić, Ivana
AU  - Milošević, Nebojša
AU  - Marić, Slađana
AU  - Lukić, Milan
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4216
AB  - Factors such as the stage of ovule maturity and longevity at flowering, when the stigma is able to receive the pollen, are significant in terms of fruit set. Various abnormalities during ovule development can often limit pollination, effective pollination period (EPP) and fruit set. In the plum cultivar 'Pozna Plava' (Prunus domestica L.), in the ovaries of open-pollinated flowers and of emasculated unpollinated flowers, we investigated abnormalities in the tissues of primary and secondary ovules during the time of anthesis until 10 days thereafter. At the same time, the viability of the ovules of the unpollinated flowers was examined. Abnormal ovules were considered those with degenerations in the egg apparatus, inverse arrangement of egg cells, more than eight nuclei in the embryo sac as well as degeneration of the nucellus and integuments. In primary ovules, the most frequent abnormality in open-pollinated and unpollinated flowers was the degeneration of the egg apparatus, with frequencies of 14.3-50.0% and 10.0-89.0%, respectively. In the secondary ovules, in both treatments, a completely different picture was obtained. The processes that led to abnormalities in the structure of the secondary ovules first occurred in the cells of the nucellus, while the elements of the embryo sac had a relatively normal cytological organisation. However, the observed abnormalities do not explain the low fruit set of this cultivar.
PB  - Elsevier Science Bv, Amsterdam
T2  - Scientia Horticulturae
T1  - Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis
EP  - 228
SP  - 222
VL  - 252
DO  - 10.1016/j.scienta.2019.03.059
ER  - 

@article{
author = "Đorđević, Milena and Cerović, Radosav and Radičević, Sanja and Glišić, Ivana and Milošević, Nebojša and Marić, Slađana and Lukić, Milan",
year = "2019",
abstract = "Factors such as the stage of ovule maturity and longevity at flowering, when the stigma is able to receive the pollen, are significant in terms of fruit set. Various abnormalities during ovule development can often limit pollination, effective pollination period (EPP) and fruit set. In the plum cultivar 'Pozna Plava' (Prunus domestica L.), in the ovaries of open-pollinated flowers and of emasculated unpollinated flowers, we investigated abnormalities in the tissues of primary and secondary ovules during the time of anthesis until 10 days thereafter. At the same time, the viability of the ovules of the unpollinated flowers was examined. Abnormal ovules were considered those with degenerations in the egg apparatus, inverse arrangement of egg cells, more than eight nuclei in the embryo sac as well as degeneration of the nucellus and integuments. In primary ovules, the most frequent abnormality in open-pollinated and unpollinated flowers was the degeneration of the egg apparatus, with frequencies of 14.3-50.0% and 10.0-89.0%, respectively. In the secondary ovules, in both treatments, a completely different picture was obtained. The processes that led to abnormalities in the structure of the secondary ovules first occurred in the cells of the nucellus, while the elements of the embryo sac had a relatively normal cytological organisation. However, the observed abnormalities do not explain the low fruit set of this cultivar.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Scientia Horticulturae",
title = "Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis",
pages = "228-222",
volume = "252",
doi = "10.1016/j.scienta.2019.03.059"
}

Đorđević, M., Cerović, R., Radičević, S., Glišić, I., Milošević, N., Marić, S.,& Lukić, M.. (2019). Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis. in Scientia Horticulturae
Elsevier Science Bv, Amsterdam., 252, 222-228.
https://doi.org/10.1016/j.scienta.2019.03.059

Đorđević M, Cerović R, Radičević S, Glišić I, Milošević N, Marić S, Lukić M. Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis. in Scientia Horticulturae. 2019;252:222-228.
doi:10.1016/j.scienta.2019.03.059 .

Đorđević, Milena, Cerović, Radosav, Radičević, Sanja, Glišić, Ivana, Milošević, Nebojša, Marić, Slađana, Lukić, Milan, "Abnormalities in the ovule development of the European plum cultivar 'Pozna Plava' in the days following anthesis" in Scientia Horticulturae, 252 (2019):222-228,
https://doi.org/10.1016/j.scienta.2019.03.059 . .