In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods
2022
Преузимање 🢃
Аутори
Ðorđević, MilenaVujović, Tatjana
Cerović, Radosav
Glišić, Ivana
Milošević, Nebojša
Marić, Slađana
Radičević, Sanja
Fotirić Akšić, Milica
Meland, Mekjell
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−2...0, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Кључне речи:
European plum / low and sub-zero temperatures / pollen germination in vitro / pollen storage / pollen viabilityИзвор:
Horticulturae, 2022, 8, 7Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200215 (Институт за воћарство, Чачак) (RS-MESTD-inst-2020-200215)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
DOI: 10.3390/horticulturae8070616
ISSN: 2311-7524
WoS: 000833082300001
Scopus: 2-s2.0-85133745921
Институција/група
Inovacioni centarTY - JOUR AU - Ðorđević, Milena AU - Vujović, Tatjana AU - Cerović, Radosav AU - Glišić, Ivana AU - Milošević, Nebojša AU - Marić, Slađana AU - Radičević, Sanja AU - Fotirić Akšić, Milica AU - Meland, Mekjell PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5176 AB - A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−20, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland. T2 - Horticulturae T2 - Horticulturae T1 - In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods IS - 7 VL - 8 DO - 10.3390/horticulturae8070616 ER -
@article{ author = "Ðorđević, Milena and Vujović, Tatjana and Cerović, Radosav and Glišić, Ivana and Milošević, Nebojša and Marić, Slađana and Radičević, Sanja and Fotirić Akšić, Milica and Meland, Mekjell", year = "2022", abstract = "A study was conducted to investigate the effect of different storage periods and temperatures on pollen viability in vitro and in vivo in plum genotypes ‘Valerija’, ‘Čačanska Lepotica’ and ‘Valjevka’. In vitro pollen viability was tested at day 0 (fresh dry pollen) and after 3, 6, 9 and 12 months of storage at four different temperatures (4, −20, −80 and −196◦C), and in vivo after 12 months of storage at distinct temperatures. In vitro germination and fluorescein diacetate (FDA) staining methods were used to test pollen viability, while aniline blue staining was used for observing in vivo pollen tube growth. Fresh pollen germination and viability ranged from 42.35 to 63.79% (‘Valjevka’ and ‘Čačanska Lepotica’, respectively) and 54.58 to 62.15%, (‘Valjevka’ and ‘Valerija’, respectively). With storage at 4◦C, pollen viability and germination decreased over the period, with the lowest value after 12 months of storage. Pollen germination and viability for the other storage temperatures (−20, −80 and −196◦C) were higher than 30% by the end of the 12 months. Pollination using pollen stored at 4◦C showed that pollen tube growth mostly ended in the lower part of the style. With the other storage temperatures, pollen tube growth was similar, ranging between 50 and 100% of the pistils with pollen tubes penetrated into the nucellus of the ovule in the genotype ‘Čačanska Lepotica’. The results of these findings will have implications for plum pollen breeding and conservation. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.", journal = "Horticulturae, Horticulturae", title = "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods", number = "7", volume = "8", doi = "10.3390/horticulturae8070616" }
Ðorđević, M., Vujović, T., Cerović, R., Glišić, I., Milošević, N., Marić, S., Radičević, S., Fotirić Akšić, M.,& Meland, M.. (2022). In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods. in Horticulturae, 8(7). https://doi.org/10.3390/horticulturae8070616
Ðorđević M, Vujović T, Cerović R, Glišić I, Milošević N, Marić S, Radičević S, Fotirić Akšić M, Meland M. In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods. in Horticulturae. 2022;8(7). doi:10.3390/horticulturae8070616 .
Ðorđević, Milena, Vujović, Tatjana, Cerović, Radosav, Glišić, Ivana, Milošević, Nebojša, Marić, Slađana, Radičević, Sanja, Fotirić Akšić, Milica, Meland, Mekjell, "In Vitro and In Vivo Performance of Plum (Prunus domestica L.) Pollen from the Anthers Stored at Distinct Temperatures for Different Periods" in Horticulturae, 8, no. 7 (2022), https://doi.org/10.3390/horticulturae8070616 . .