TY  - CONF
AU  - Knežević, Magdalena
AU  - Stajković-Srbinović, Olivera
AU  - Maibassova, Assel
AU  - Milić, Marija
AU  - Mihajlovski, Katarina
AU  - Delić, Dušica
AU  - Buntić, Aneta
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6434
AB  - The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.
PB  - MDPI
C3  - 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
T1  - Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6434
ER  - 

@conference{
author = "Knežević, Magdalena and Stajković-Srbinović, Olivera and Maibassova, Assel and Milić, Marija and Mihajlovski, Katarina and Delić, Dušica and Buntić, Aneta",
year = "2022",
abstract = "The preference to use biological control agents to control plant diseases instead of fungicides has arisen worldwide. The aim of this research was to isolate and characterise pseudomonades effective against Fusarium oxysporum and to test its potential in improving infected oat seed germination in vitro. Isolation of bacteria was performed from rhizosphere of Vigna radiata and Medicago sativa on King¢s B medium. Molecular characterisation of isolates was done based on the 16S rRNA sequence (using P0/P6 primers), while the presence of phenazine genes was tested by PCR method using PHZEf/PHZEr primers. Antifungal activity of bacterial isolates against F. oxysporum, as well as the production of HCN and siderophores was tested in vitro. Potential of selected Pseudomonas sp. inoculums to increase relative seed germination index (RSGI, %) of seeds infected with F. oxysporum was tested. Two isolates from V. radiata (Vig3Psd1 and Vig3Psd2) and two from M. sativa (LA1Psd1 and LA1Psd2) rhizosphere were characterised as Pseudomonas sp. based on the molecular determination. Production of siderophores was recorded for all tested isolates, with LA1Psd1 as the most potent producer (20 mm halo zone), while the HCN was recorded for Vig3Psd2 and LA1Psd2. The presence of genes for phenazine production was determined only for Vig3Psd2. Inoculation of infected oat seeds with all selected inoculums increased RSGI in a range from 21% (LA1Psd2) to 33% (Vig3Psd2), in comparison to the infected control. The results indicated that inoculum based on Vig3Psd2 has potential to be used in green farming, after further greenhouse and field experiments.",
publisher = "MDPI",
journal = "1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection",
title = "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6434"
}

Knežević, M., Stajković-Srbinović, O., Maibassova, A., Milić, M., Mihajlovski, K., Delić, D.,& Buntić, A.. (2022). Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection
MDPI..
https://hdl.handle.net/21.15107/rcub_technorep_6434

Knežević M, Stajković-Srbinović O, Maibassova A, Milić M, Mihajlovski K, Delić D, Buntić A. Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades. in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

Knežević, Magdalena, Stajković-Srbinović, Olivera, Maibassova, Assel, Milić, Marija, Mihajlovski, Katarina, Delić, Dušica, Buntić, Aneta, "Suppression of Fusarium oxysporum infection in oat (Avena sativa) seeds by rhizosphere-associated pseudomonades" in 1st International Online Conference on Agriculture - Advances in Agricultural Science and Technology, Section: Zero-Pollution Solutions in Crop Protection (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6434 .

TY  - CONF
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera
AU  - Milić, Marija
AU  - Kuzmanović, Đorđe
AU  - Rasulić, Nataša
AU  - Dimitrijević-Branković, Suzana
AU  - Delić, Dušica
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6425
AB  - Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.
PB  - Soil Science Society of Kazakhstan
PB  - Federation of Eurasian Soil Science Societies
C3  - 10th International Soil Science Congress on "Environment and Soil Resources Conservation
T1  - Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates
EP  - 200
SP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6425
ER  - 

@conference{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Kuzmanović, Đorđe and Rasulić, Nataša and Dimitrijević-Branković, Suzana and Delić, Dušica",
year = "2018",
abstract = "Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.",
publisher = "Soil Science Society of Kazakhstan, Federation of Eurasian Soil Science Societies",
journal = "10th International Soil Science Congress on "Environment and Soil Resources Conservation",
title = "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates",
pages = "200-197",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6425"
}

Buntić, A., Stajković-Srbinović, O., Milić, M., Kuzmanović, Đ., Rasulić, N., Dimitrijević-Branković, S.,& Delić, D.. (2018). Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation
Soil Science Society of Kazakhstan., 197-200.
https://hdl.handle.net/21.15107/rcub_technorep_6425

Buntić A, Stajković-Srbinović O, Milić M, Kuzmanović Đ, Rasulić N, Dimitrijević-Branković S, Delić D. Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation. 2018;:197-200.
https://hdl.handle.net/21.15107/rcub_technorep_6425 .

Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Kuzmanović, Đorđe, Rasulić, Nataša, Dimitrijević-Branković, Suzana, Delić, Dušica, "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates" in 10th International Soil Science Congress on "Environment and Soil Resources Conservation (2018):197-200,
https://hdl.handle.net/21.15107/rcub_technorep_6425 .