Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates
Само за регистроване кориснике
2018
Аутори
Buntić, AnetaStajković-Srbinović, Olivera
Milić, Marija
Kuzmanović, Đorđe
Rasulić, Nataša
Dimitrijević-Branković, Suzana
Delić, Dušica
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, afte...r 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.
Кључне речи:
cellulase production / waste material utilization / Avicelase activity / rhizobiumИзвор:
10th International Soil Science Congress on "Environment and Soil Resources Conservation, 2018, 197-200Издавач:
- Soil Science Society of Kazakhstan
- Federation of Eurasian Soil Science Societies
Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
- Проучавање утицаја квалитета земљишта и вода за наводњавање на ефикаснију производњу пољопривредних култура и очување животне средине (RS-MESTD-Technological Development (TD or TR)-37006)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Buntić, Aneta AU - Stajković-Srbinović, Olivera AU - Milić, Marija AU - Kuzmanović, Đorđe AU - Rasulić, Nataša AU - Dimitrijević-Branković, Suzana AU - Delić, Dušica PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6425 AB - Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste. PB - Soil Science Society of Kazakhstan PB - Federation of Eurasian Soil Science Societies C3 - 10th International Soil Science Congress on "Environment and Soil Resources Conservation T1 - Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates EP - 200 SP - 197 UR - https://hdl.handle.net/21.15107/rcub_technorep_6425 ER -
@conference{ author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Kuzmanović, Đorđe and Rasulić, Nataša and Dimitrijević-Branković, Suzana and Delić, Dušica", year = "2018", abstract = "Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.", publisher = "Soil Science Society of Kazakhstan, Federation of Eurasian Soil Science Societies", journal = "10th International Soil Science Congress on "Environment and Soil Resources Conservation", title = "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates", pages = "200-197", url = "https://hdl.handle.net/21.15107/rcub_technorep_6425" }
Buntić, A., Stajković-Srbinović, O., Milić, M., Kuzmanović, Đ., Rasulić, N., Dimitrijević-Branković, S.,& Delić, D.. (2018). Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation Soil Science Society of Kazakhstan., 197-200. https://hdl.handle.net/21.15107/rcub_technorep_6425
Buntić A, Stajković-Srbinović O, Milić M, Kuzmanović Đ, Rasulić N, Dimitrijević-Branković S, Delić D. Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation. 2018;:197-200. https://hdl.handle.net/21.15107/rcub_technorep_6425 .
Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Kuzmanović, Đorđe, Rasulić, Nataša, Dimitrijević-Branković, Suzana, Delić, Dušica, "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates" in 10th International Soil Science Congress on "Environment and Soil Resources Conservation (2018):197-200, https://hdl.handle.net/21.15107/rcub_technorep_6425 .