TY  - JOUR
AU  - Marković, Maja D.
AU  - Tadić, Julijana D.
AU  - Savić, Sanja I.
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
AU  - Mijin, Dušan
AU  - Panić, Vesna V.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5136
AB  - Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.
PB  - John Wiley and Sons Inc
T2  - Journal of Biomedical Materials Research - Part A
T1  - Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment
DO  - 10.1002/jbm.a.37396
ER  - 

@article{
author = "Marković, Maja D. and Tadić, Julijana D. and Savić, Sanja I. and Matić, Ivana Z. and Stanojković, Tatjana P. and Mijin, Dušan and Panić, Vesna V.",
year = "2022",
abstract = "Researchers are faced with everyday demands for safer and more efficient therapy for many diseases, especially serious one such as various types of cancer. Numerous anticancer drugs are poorly-water soluble and therefore their encapsulation and controlled release remain quite challenge. In present study, we deepened our research of hydrophilic carrier based on poly(methacrylic acid) and casein (PMAC) by investigating its potential for encapsulation and controlled release of novel poorly water-soluble dihydropyrimidion-azo-pyridon compound (DHPMP). DHPMP is a dye that has been proven to show cytotoxic activity against chronic myeloid leukemia K562 cells. By encapsulating DHPMP into the carrier and delivering it into the intestines, DHPMP absorption could be the fastest and the number of therapeutic doses and side effects can be reduced. Carriers based on PMAC and DHPMP (PMAC-DHPMP) were synthetized and characterized by FTIR, SEM and single compression tests. The swelling behavior of PMAC-DHPMP carriers and cumulative DHPMP release were investigated depending on the amount of crosslinker and encapsulated DHPMP in two media which were simulating pH environments in human stomach and intestines. The prolonged and controlled release of DHPMP was achieved. In vitro cytotoxic activity of PMAC-DHPMP carriers against K562 cells and the cell cycle analysis showed great potential of the carriers for application in leukemia treatment.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment",
doi = "10.1002/jbm.a.37396"
}

Marković, M. D., Tadić, J. D., Savić, S. I., Matić, I. Z., Stanojković, T. P., Mijin, D.,& Panić, V. V.. (2022). Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A
John Wiley and Sons Inc..
https://doi.org/10.1002/jbm.a.37396

Marković MD, Tadić JD, Savić SI, Matić IZ, Stanojković TP, Mijin D, Panić VV. Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment. in Journal of Biomedical Materials Research - Part A. 2022;.
doi:10.1002/jbm.a.37396 .

Marković, Maja D., Tadić, Julijana D., Savić, Sanja I., Matić, Ivana Z., Stanojković, Tatjana P., Mijin, Dušan, Panić, Vesna V., "Soft 3D hybrid network for delivery and controlled release of poorly soluble dihydropyrimidinone compound: An insight into the novel system for potential application in leukemia treatment" in Journal of Biomedical Materials Research - Part A (2022),
https://doi.org/10.1002/jbm.a.37396 . .

TY  - CONF
AU  - Tadić, Julijana
AU  - Lađarević, Jelena
AU  - Stanojković, Tatjana
AU  - Matić, Ivana
AU  - Mijin, Dušan
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6754
AB  - In recent years, the growing interest in the synthesis of heterocyclic azo dyes can be assigned to a versatile application and biological activities such as antibacterial, antifungal, antioxidant, analgesic, antitubercular and anticancer, of these compounds. Azo dyes based on 2-pyridone core are important since they manifest a variety of biological properties, including potency for the cancer therapy. Moreover, DHPM (3,4-dihydropyrimidin-2(1H)-on) scaffold displays a wide range of biological activities, which has led to the development of numerous drug candidates, based on this structure. Since the cancer is one of the main targets in therapeutic chemistry, we have made an effort to design azo molecules with potential anticancer activity. In this direction, we have synthesized DHPM scaffold suitable for diazo coupling reaction with different 2-pyridones, in order to design a novel series of azo pyridone molecules. The chemical structures of obtained dyes have been confirmed by ATR-FTIR, NMR, UV-Vis, MS and elemental analysis. The antioxidant activity has been evaluated by ABTS (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) assay. In vitro antitumor action of DHPM precursor and related azo dyes was determined against prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562) tumor cell lines, as well as against human normal lung fibroblast (MRC-5), using MTT (microculture tetrazolium test) assay. The physicochemical parameters, druglikeness and ADME properties of novel compounds were evaluated in silico by SwissADME. The ATR-FTIR and NMR spectroscopy confirmed that obtained dyes exist in hydrazone tautomeric form. The antioxidant assay evinced that three members of this dyes’ series are promising antioxidant candidates. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. It has been noted that cytotoxic activity of investigated dyes depends on the structure of pyridone moiety since DHPM adduct expressed low cytotoxic activity against all investigated cancer cell lines. All tested cancer cell lines were the most sensitive to the cytotoxic action of compound carrying 4-phenyl group in the pyridone moiety. Furthermore, this compound was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. The last but not the least, ADME evaluation in silico has shown that all investigated compounds may be orally bioavailable. Altogether, it can be concluded that designed dyes’ molecules may serve for further structural modification and development of new anticancer drugs.
PB  - Zvornik : University of East Sarajevo Faculty of Technology
C3  - Book of Abstracts / VII International Congress “Engineering, Environment and Materials in Process Industry” EEM 2021, 17-19 mart 2021, Jahorina, Bosna i Hercegovina
T1  - IN VITRO ANTICANCER ACTIVITY STUDY ON SOME AZO PYRIDONE DYES DERIVED FROM DHPM SCAFFOLD
SP  - 243
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6754
ER  - 

@conference{
author = "Tadić, Julijana and Lađarević, Jelena and Stanojković, Tatjana and Matić, Ivana and Mijin, Dušan",
year = "2021",
abstract = "In recent years, the growing interest in the synthesis of heterocyclic azo dyes can be assigned to a versatile application and biological activities such as antibacterial, antifungal, antioxidant, analgesic, antitubercular and anticancer, of these compounds. Azo dyes based on 2-pyridone core are important since they manifest a variety of biological properties, including potency for the cancer therapy. Moreover, DHPM (3,4-dihydropyrimidin-2(1H)-on) scaffold displays a wide range of biological activities, which has led to the development of numerous drug candidates, based on this structure. Since the cancer is one of the main targets in therapeutic chemistry, we have made an effort to design azo molecules with potential anticancer activity. In this direction, we have synthesized DHPM scaffold suitable for diazo coupling reaction with different 2-pyridones, in order to design a novel series of azo pyridone molecules. The chemical structures of obtained dyes have been confirmed by ATR-FTIR, NMR, UV-Vis, MS and elemental analysis. The antioxidant activity has been evaluated by ABTS (2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)) assay. In vitro antitumor action of DHPM precursor and related azo dyes was determined against prostate adenocarcinoma (PC-3), lung carcinoma (A549) and chronic myelogenous leukemia (K562) tumor cell lines, as well as against human normal lung fibroblast (MRC-5), using MTT (microculture tetrazolium test) assay. The physicochemical parameters, druglikeness and ADME properties of novel compounds were evaluated in silico by SwissADME. The ATR-FTIR and NMR spectroscopy confirmed that obtained dyes exist in hydrazone tautomeric form. The antioxidant assay evinced that three members of this dyes’ series are promising antioxidant candidates. Examination of cytotoxic effects on human cancer cell lines showed the concentration dependent cytotoxicity of all investigated compounds. It has been noted that cytotoxic activity of investigated dyes depends on the structure of pyridone moiety since DHPM adduct expressed low cytotoxic activity against all investigated cancer cell lines. All tested cancer cell lines were the most sensitive to the cytotoxic action of compound carrying 4-phenyl group in the pyridone moiety. Furthermore, this compound was particularly prominent and selective in cytotoxic action between K562 (24.97 μM) and PC-3 (48.98 μM) cancer cells, and normal MRC-5 (91.11 μM) cells. The last but not the least, ADME evaluation in silico has shown that all investigated compounds may be orally bioavailable. Altogether, it can be concluded that designed dyes’ molecules may serve for further structural modification and development of new anticancer drugs.",
publisher = "Zvornik : University of East Sarajevo Faculty of Technology",
journal = "Book of Abstracts / VII International Congress “Engineering, Environment and Materials in Process Industry” EEM 2021, 17-19 mart 2021, Jahorina, Bosna i Hercegovina",
title = "IN VITRO ANTICANCER ACTIVITY STUDY ON SOME AZO PYRIDONE DYES DERIVED FROM DHPM SCAFFOLD",
pages = "243",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6754"
}

Tadić, J., Lađarević, J., Stanojković, T., Matić, I.,& Mijin, D.. (2021). IN VITRO ANTICANCER ACTIVITY STUDY ON SOME AZO PYRIDONE DYES DERIVED FROM DHPM SCAFFOLD. in Book of Abstracts / VII International Congress “Engineering, Environment and Materials in Process Industry” EEM 2021, 17-19 mart 2021, Jahorina, Bosna i Hercegovina
Zvornik : University of East Sarajevo Faculty of Technology., 243.
https://hdl.handle.net/21.15107/rcub_technorep_6754

Tadić J, Lađarević J, Stanojković T, Matić I, Mijin D. IN VITRO ANTICANCER ACTIVITY STUDY ON SOME AZO PYRIDONE DYES DERIVED FROM DHPM SCAFFOLD. in Book of Abstracts / VII International Congress “Engineering, Environment and Materials in Process Industry” EEM 2021, 17-19 mart 2021, Jahorina, Bosna i Hercegovina. 2021;:243.
https://hdl.handle.net/21.15107/rcub_technorep_6754 .

Tadić, Julijana, Lađarević, Jelena, Stanojković, Tatjana, Matić, Ivana, Mijin, Dušan, "IN VITRO ANTICANCER ACTIVITY STUDY ON SOME AZO PYRIDONE DYES DERIVED FROM DHPM SCAFFOLD" in Book of Abstracts / VII International Congress “Engineering, Environment and Materials in Process Industry” EEM 2021, 17-19 mart 2021, Jahorina, Bosna i Hercegovina (2021):243,
https://hdl.handle.net/21.15107/rcub_technorep_6754 .

TY  - JOUR
AU  - Durdević, Sanja
AU  - Šavikin, Katarina
AU  - Živković, Jelena Č.
AU  - Boehm, Volker
AU  - Stanojković, Tatjana
AU  - Damjanović, Ana
AU  - Petrović, Slobodan
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3961
AB  - Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.
EP  - 232
SP  - 225
VL  - 133
DO  - 10.1016/j.supflu.2017.10.021
ER  - 

@article{
author = "Durdević, Sanja and Šavikin, Katarina and Živković, Jelena Č. and Boehm, Volker and Stanojković, Tatjana and Damjanović, Ana and Petrović, Slobodan",
year = "2018",
abstract = "Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.",
pages = "232-225",
volume = "133",
doi = "10.1016/j.supflu.2017.10.021"
}

Durdević, S., Šavikin, K., Živković, J. Č., Boehm, V., Stanojković, T., Damjanović, A.,& Petrović, S.. (2018). Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 133, 225-232.
https://doi.org/10.1016/j.supflu.2017.10.021

Durdević S, Šavikin K, Živković JČ, Boehm V, Stanojković T, Damjanović A, Petrović S. Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids. 2018;133:225-232.
doi:10.1016/j.supflu.2017.10.021 .

Durdević, Sanja, Šavikin, Katarina, Živković, Jelena Č., Boehm, Volker, Stanojković, Tatjana, Damjanović, Ana, Petrović, Slobodan, "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L." in Journal of Supercritical Fluids, 133 (2018):225-232,
https://doi.org/10.1016/j.supflu.2017.10.021 . .

TY  - JOUR
AU  - Knežević, Aleksandar
AU  - Stajić, Mirjana
AU  - Sofrenić, Ivana V.
AU  - Stašić, Jelena M.
AU  - Milovanović, Ivan
AU  - Tešević, Vele
AU  - Vukojević, Jelena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3849
AB  - In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolorwas studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by H-1 NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS(+) scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia
IS  - 8
VL  - 13
DO  - 10.1371/journal.pone.0203064
ER  - 

@article{
author = "Knežević, Aleksandar and Stajić, Mirjana and Sofrenić, Ivana V. and Stašić, Jelena M. and Milovanović, Ivan and Tešević, Vele and Vukojević, Jelena",
year = "2018",
abstract = "In a last few decades mushrooms are increasingly attracting attention as functional food and sources of biologically active compounds. Several Trametes species have been used for centuries in traditional medicine of East Asia cultures, but only T. versicolorwas studied sufficiently while there are less substantial data about medicinal properties of other species. Trametes versicolor, T. hirsuta and T. gibbosa were the species tested for biological activities. Antifungal potentials of extracts were assessed for clinical strains of selected Candida and Aspergillus species. ABTS and FRAP assays were used to evaluate antioxidant capacities of studied extracts. Cytotoxic activity was determined against human cervix and lung adenocarcinoma and colon carcinoma cell lines. Antineurodegenerative activity was assessed by determining the rate of acetylcholinesterase and tyrosinase activity. The presence of metabolites in extracts of mycelia and basidiocarps of studied Trametes species was analyzed by H-1 NMR spectroscopy. Studied extracts showed low antifungal potential in comparison with ketoconazole. Basidiocarp extracts were more effective ABTS(+) scavengers and Fe2+ reducers than mycelium ones but less effective in comparison with L-ascorbic acid. Results showed that mycelium extracts had stronger cytotoxic effects against three cancer cell lines than basidiocarp ones, and that cervix adenocarcinoma cells were the most sensitive to the extracts and commercial cytostatics. T. versicolor mycelium extract was the most effective inhibitor of acetylcholinesterase activity but double weaker than galantamine, and T. gibbosa mycelium extract was significantly better inhibitor of tyrosinase activity than kojic acid for 40.9%. Chemical analysis indicated strong synergistic action of triterpenes, sugars and polyphenols in applied assays. The results suggest that tested Trametes species have significant medicinal potentials which could be attributed to antioxidative and cytotoxic activity. Additionally both, basidiocarps and mycelia extracts can strongly inhibit activity of acetylcholinesterase and tyrosinase.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia",
number = "8",
volume = "13",
doi = "10.1371/journal.pone.0203064"
}

Knežević, A., Stajić, M., Sofrenić, I. V., Stašić, J. M., Milovanović, I., Tešević, V.,& Vukojević, J.. (2018). Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. in PLoS One
Public Library Science, San Francisco., 13(8).
https://doi.org/10.1371/journal.pone.0203064

Knežević A, Stajić M, Sofrenić IV, Stašić JM, Milovanović I, Tešević V, Vukojević J. Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia. in PLoS One. 2018;13(8).
doi:10.1371/journal.pone.0203064 .

Knežević, Aleksandar, Stajić, Mirjana, Sofrenić, Ivana V., Stašić, Jelena M., Milovanović, Ivan, Tešević, Vele, Vukojević, Jelena, "Antioxidative, antifungal, cytotoxic and antineurodegenerative activity of selected Trametes species from Serbia" in PLoS One, 13, no. 8 (2018),
https://doi.org/10.1371/journal.pone.0203064 . .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Stajić, Mirjana
AU  - Ćilerdžić, Jasmina
AU  - Stanojković, Tatjana
AU  - Knezević, Aleksandar
AU  - Vukojević, Jelena
PY  - 2014
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2689
AB  - The goal of this study was the evaluation of antifungal, antioxidant and anticancer potentials of Pleurotus eryngii, P. ostreatus and P. pulmonarius mycelial extracts, and the influence of mycelium enrichment with selenium on these activities. Both Se-amended and non-amended extracts showed the same or similar minimal inhibitory concentration for 14 studied micromycetes, while a fungicidal effect was not noted, contrary to ketoconazole, which had inhibitory and fungicidal effects at very low concentrations. Se-non-amended extracts exhibited antioxidant activity, especially at higher concentrations. Selenium enrichment influenced activity, its effects decreasing in P. eryngii and P. pulmonarius, while in P. ostreatus no effect was noted. The DPPH radical scavenging capacity of the extracts was in direct correlation with their phenol and flavonoid contents. Cytotoxic activity against both HeLa and LS174 cell lines was very low compared with cis-DDP. These features suggest that mycelium should be an object of intensive studies.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts
EP  - 1388
IS  - 4
SP  - 1379
VL  - 66
DO  - 10.2298/ABS1404379M
ER  - 

@article{
author = "Milovanović, Ivan and Stajić, Mirjana and Ćilerdžić, Jasmina and Stanojković, Tatjana and Knezević, Aleksandar and Vukojević, Jelena",
year = "2014",
abstract = "The goal of this study was the evaluation of antifungal, antioxidant and anticancer potentials of Pleurotus eryngii, P. ostreatus and P. pulmonarius mycelial extracts, and the influence of mycelium enrichment with selenium on these activities. Both Se-amended and non-amended extracts showed the same or similar minimal inhibitory concentration for 14 studied micromycetes, while a fungicidal effect was not noted, contrary to ketoconazole, which had inhibitory and fungicidal effects at very low concentrations. Se-non-amended extracts exhibited antioxidant activity, especially at higher concentrations. Selenium enrichment influenced activity, its effects decreasing in P. eryngii and P. pulmonarius, while in P. ostreatus no effect was noted. The DPPH radical scavenging capacity of the extracts was in direct correlation with their phenol and flavonoid contents. Cytotoxic activity against both HeLa and LS174 cell lines was very low compared with cis-DDP. These features suggest that mycelium should be an object of intensive studies.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts",
pages = "1388-1379",
number = "4",
volume = "66",
doi = "10.2298/ABS1404379M"
}

Milovanović, I., Stajić, M., Ćilerdžić, J., Stanojković, T., Knezević, A.,& Vukojević, J.. (2014). Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1379-1388.
https://doi.org/10.2298/ABS1404379M

Milovanović I, Stajić M, Ćilerdžić J, Stanojković T, Knezević A, Vukojević J. Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts. in Archives of Biological Sciences. 2014;66(4):1379-1388.
doi:10.2298/ABS1404379M .

Milovanović, Ivan, Stajić, Mirjana, Ćilerdžić, Jasmina, Stanojković, Tatjana, Knezević, Aleksandar, Vukojević, Jelena, "Antioxidant, antifungal and anticancer activities of se-enriched pleurotus spp. Mycelium extracts" in Archives of Biological Sciences, 66, no. 4 (2014):1379-1388,
https://doi.org/10.2298/ABS1404379M . .