TY  - JOUR
AU  - Vajić, Una-Jovana
AU  - Živković, Jelena
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5561
AB  - The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
EP  - 128
IS  - 1
SP  - 119
VL  - 41
DO  - 10.20450/mjcce.2022.2238
ER  - 

@article{
author = "Vajić, Una-Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology",
pages = "128-119",
number = "1",
volume = "41",
doi = "10.20450/mjcce.2022.2238"
}

Vajić, U., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 41(1), 119-128.
https://doi.org/10.20450/mjcce.2022.2238

Vajić U, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128.
doi:10.20450/mjcce.2022.2238 .

Vajić, Una-Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128,
https://doi.org/10.20450/mjcce.2022.2238 . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6409
AB  - Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries.
AB  - Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.
PB  - Serbian Nutrition Society, Belgrade
T2  - Hrana i ishrana
T1  - Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques
EP  - 20
IS  - 2
SP  - 15
VL  - 62
DO  - 10.5937/hraIsh2102015J
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries., Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.",
publisher = "Serbian Nutrition Society, Belgrade",
journal = "Hrana i ishrana",
title = "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques",
pages = "20-15",
number = "2",
volume = "62",
doi = "10.5937/hraIsh2102015J"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana
Serbian Nutrition Society, Belgrade., 62(2), 15-20.
https://doi.org/10.5937/hraIsh2102015J

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Bugarski B. Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana. 2021;62(2):15-20.
doi:10.5937/hraIsh2102015J .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques" in Hrana i ishrana, 62, no. 2 (2021):15-20,
https://doi.org/10.5937/hraIsh2102015J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6441
AB  - The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.
PB  - Serbian Nutrition Society
C3  - 14th Congress of Nutrition
T1  - The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6441
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko",
year = "2021",
abstract = "The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.",
publisher = "Serbian Nutrition Society",
journal = "14th Congress of Nutrition",
title = "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6441"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K., Nedović, V.,& Bugarski, B.. (2021). The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition
Serbian Nutrition Society..
https://hdl.handle.net/21.15107/rcub_technorep_6441

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Nedović V, Bugarski B. The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition. 2021;:null-76.
https://hdl.handle.net/21.15107/rcub_technorep_6441 .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts" in 14th Congress of Nutrition (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6441 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6410
AB  - Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Thymus serpyllum extract loaded liposomes produced by proliposome method
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6410
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Petrović, Predrag and Đorđević, Verica and Šavikin, Katarina and Zdunić, Gordana and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Thymus serpyllum extract loaded liposomes produced by proliposome method",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6410"
}

Jovanović, A. A., Balanč, B., Petrović, P., Đorđević, V., Šavikin, K., Zdunić, G.,& Bugarski, B.. (2021). Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference
University of Belgrade..
https://hdl.handle.net/21.15107/rcub_technorep_6410

Jovanović AA, Balanč B, Petrović P, Đorđević V, Šavikin K, Zdunić G, Bugarski B. Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference. 2021;:null-144.
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

Jovanović, Aleksandra A., Balanč, Bojana, Petrović, Predrag, Đorđević, Verica, Šavikin, Katarina, Zdunić, Gordana, Bugarski, Branko, "Thymus serpyllum extract loaded liposomes produced by proliposome method" in 2nd International UNIfood Conference (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Mijin, Dušan
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6443
AB  - Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.
PB  - Faculty of Technology, Zvornik
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
T1  - The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme
EP  - 143
SP  - 137
DO  - 10.7251/EEMEN2101137J
ER  - 

@conference{
author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Mijin, Dušan and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.",
publisher = "Faculty of Technology, Zvornik",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM",
title = "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme",
pages = "143-137",
doi = "10.7251/EEMEN2101137J"
}

Jovanović, A., Petrović, P., Đorđević, V., Mijin, D., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
Faculty of Technology, Zvornik., 137-143.
https://doi.org/10.7251/EEMEN2101137J

Jovanović A, Petrović P, Đorđević V, Mijin D, Zdunić G, Šavikin K, Bugarski B. The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM. 2021;:137-143.
doi:10.7251/EEMEN2101137J .

Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Mijin, Dušan, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme" in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM (2021):137-143,
https://doi.org/10.7251/EEMEN2101137J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Trajković, Milica
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Živković, Jelena
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6411
AB  - Rosa canina pseudo-fruit is rich in bioactive substances, such are carotenoids, ascorbic acid,
polyphenols, fatty acids and minerals. Because of its diuretic, anti-inflammatory, anti-allergic and
antioxidant properties it is frequently employed in traditional medicine. However, in order to
protect mentioned bioactive compounds and to improve its bioavailability and controlled release,
numerous encapsulation methods have been developed. Thus, R. canina ethanol extract obtained
using percolation at ambient temperature (25°C), was encapsulated into Lipoid G liposomes
produced in thin film procedure. Total polyphenol and flavonoid contents, as well as antioxidant
potential of the extract and extract loaded liposomes were determined. Encapsulation efficiency,
particle size, polydispersity index and zeta potential of the liposomes with extract were monitoring
during 30 days. Total polyphenol and flavonoid contents of the extract were 103.8±1.9 mg of gallic
acid equivalents/g of the extract and 64.2±2.4 mg of catechin equivalents/g of the extract,
respectively; antioxidant potential of the extract was 15.3±2.1 mg of Trolox equivalents/g of the
extract (ABTS assay) and 1.07±0.01 mg/mL of the extract (DPPH assay). Total polyphenol and
flavonoid contents of the extract loaded liposomes were 0.316±0.023 mg of gallic acid
equivalents/mg of lipids and 0.219±0.009 mg of catechin equivalents/mg of lipids, respectively;
antioxidant potential of the sample was 0.134±0.004 mg of Trolox equivalents/mg of lipids (ABTS
assay) and 270.6±10.5 mg/mL of liposomal suspension (DPPH assay). Encapsulation efficiency
was the same during monitoring period and it was amounted 46.6±3.4%. Particle size and
polydispersity index of the liposomes with extract were increased from 618.2±10.9 to 1698.0±104.2
nm and from 0.441±0.007 to 0.589±0.011, respectively, during 30 days. On the other hand, zeta
potential of the extract loaded liposomes was decreased from -10.24±0.95 to -7.21±0.77 mV. R.
canina extract loaded liposomes developed in this study have potential to be used in food,
pharmacological and cosmetic industries due to beneficial health effects of R. canina active
compounds encapsulated into liposomes.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Encapsulation of Rosa canina extract in liposomes produced by thin film method
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6411
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Trajković, Milica and Đorđević, Verica and Šavikin, Katarina and Živković, Jelena and Bugarski, Branko",
year = "2021",
abstract = "Rosa canina pseudo-fruit is rich in bioactive substances, such are carotenoids, ascorbic acid,
polyphenols, fatty acids and minerals. Because of its diuretic, anti-inflammatory, anti-allergic and
antioxidant properties it is frequently employed in traditional medicine. However, in order to
protect mentioned bioactive compounds and to improve its bioavailability and controlled release,
numerous encapsulation methods have been developed. Thus, R. canina ethanol extract obtained
using percolation at ambient temperature (25°C), was encapsulated into Lipoid G liposomes
produced in thin film procedure. Total polyphenol and flavonoid contents, as well as antioxidant
potential of the extract and extract loaded liposomes were determined. Encapsulation efficiency,
particle size, polydispersity index and zeta potential of the liposomes with extract were monitoring
during 30 days. Total polyphenol and flavonoid contents of the extract were 103.8±1.9 mg of gallic
acid equivalents/g of the extract and 64.2±2.4 mg of catechin equivalents/g of the extract,
respectively; antioxidant potential of the extract was 15.3±2.1 mg of Trolox equivalents/g of the
extract (ABTS assay) and 1.07±0.01 mg/mL of the extract (DPPH assay). Total polyphenol and
flavonoid contents of the extract loaded liposomes were 0.316±0.023 mg of gallic acid
equivalents/mg of lipids and 0.219±0.009 mg of catechin equivalents/mg of lipids, respectively;
antioxidant potential of the sample was 0.134±0.004 mg of Trolox equivalents/mg of lipids (ABTS
assay) and 270.6±10.5 mg/mL of liposomal suspension (DPPH assay). Encapsulation efficiency
was the same during monitoring period and it was amounted 46.6±3.4%. Particle size and
polydispersity index of the liposomes with extract were increased from 618.2±10.9 to 1698.0±104.2
nm and from 0.441±0.007 to 0.589±0.011, respectively, during 30 days. On the other hand, zeta
potential of the extract loaded liposomes was decreased from -10.24±0.95 to -7.21±0.77 mV. R.
canina extract loaded liposomes developed in this study have potential to be used in food,
pharmacological and cosmetic industries due to beneficial health effects of R. canina active
compounds encapsulated into liposomes.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Encapsulation of Rosa canina extract in liposomes produced by thin film method",
pages = "176",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6411"
}

Jovanović, A. A., Balanč, B., Trajković, M., Đorđević, V., Šavikin, K., Živković, J.,& Bugarski, B.. (2021). Encapsulation of Rosa canina extract in liposomes produced by thin film method. in 2nd International UNIfood Conference
University of Belgrade..
https://hdl.handle.net/21.15107/rcub_technorep_6411

Jovanović AA, Balanč B, Trajković M, Đorđević V, Šavikin K, Živković J, Bugarski B. Encapsulation of Rosa canina extract in liposomes produced by thin film method. in 2nd International UNIfood Conference. 2021;:null-176.
https://hdl.handle.net/21.15107/rcub_technorep_6411 .

Jovanović, Aleksandra A., Balanč, Bojana, Trajković, Milica, Đorđević, Verica, Šavikin, Katarina, Živković, Jelena, Bugarski, Branko, "Encapsulation of Rosa canina extract in liposomes produced by thin film method" in 2nd International UNIfood Conference (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6411 .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Skrt, Mihaela
AU  - Petrović, Predrag
AU  - Janković-Častvan, Ivona
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4288
AB  - The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity
EP  - 29
IS  - 39
SP  - 23
DO  - 10.5937/leksir1939023J
ER  - 

@article{
author = "Jovanović, Aleksandra and Skrt, Mihaela and Petrović, Predrag and Janković-Častvan, Ivona and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2019",
abstract = "The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme (Thymus serpyllum L.) as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio, and extraction technique have shown statistically significant influence, whereas the exposure time has not been a relevant factor. In all three extraction procedures (maceration, heat-and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly higher after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)-ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction  gt  heat-assisted extraction , while in 2,2-diphenyl-1-picrylhydrazyl-DPPH neutralization: ultrasound-assisted extraction  gt  heat-assisted extraction  gt  gt  maceration. The study represents an important step in the formulation of antioxidant-rich extracts with potential use in food, pharmaceutical, and cosmetic industry.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity",
pages = "29-23",
number = "39",
doi = "10.5937/leksir1939023J"
}

Jovanović, A., Skrt, M., Petrović, P., Janković-Častvan, I., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2019). Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(39), 23-29.
https://doi.org/10.5937/leksir1939023J

Jovanović A, Skrt M, Petrović P, Janković-Častvan I, Zdunić G, Šavikin K, Bugarski B. Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity. in Lekovite sirovine. 2019;(39):23-29.
doi:10.5937/leksir1939023J .

Jovanović, Aleksandra, Skrt, Mihaela, Petrović, Predrag, Janković-Častvan, Ivona, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity" in Lekovite sirovine, no. 39 (2019):23-29,
https://doi.org/10.5937/leksir1939023J . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Ota, Ajda
AU  - Skrt, Mihaela
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Nedović, Viktor
AU  - Poklar-Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4088
AB  - Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol
VL  - 183
DO  - 10.1016/j.colsurfb.2019.110422
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Đorđević, Verica and Ota, Ajda and Skrt, Mihaela and Šavikin, Katarina and Bugarski, Branko and Nedović, Viktor and Poklar-Ulrih, Nataša",
year = "2019",
abstract = "Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol",
volume = "183",
doi = "10.1016/j.colsurfb.2019.110422"
}

Jovanović, A., Balanč, B., Đorđević, V., Ota, A., Skrt, M., Šavikin, K., Bugarski, B., Nedović, V.,& Poklar-Ulrih, N.. (2019). Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 183.
https://doi.org/10.1016/j.colsurfb.2019.110422

Jovanović A, Balanč B, Đorđević V, Ota A, Skrt M, Šavikin K, Bugarski B, Nedović V, Poklar-Ulrih N. Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces. 2019;183.
doi:10.1016/j.colsurfb.2019.110422 .

Jovanović, Aleksandra, Balanč, Bojana, Đorđević, Verica, Ota, Ajda, Skrt, Mihaela, Šavikin, Katarina, Bugarski, Branko, Nedović, Viktor, Poklar-Ulrih, Nataša, "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol" in Colloids and Surfaces B-Biointerfaces, 183 (2019),
https://doi.org/10.1016/j.colsurfb.2019.110422 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Poklar-Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4302
AB  - The present study provides insight into changes in the liposomal membrane fluidity as a result of variation of structural components (two types of standard phospholipids - 1,2-dipalmitoyl-sn-glycerero3-phosphocholine, i.e. DPPC and 1-palmitoyl-2-oleoylglycerol-3-phosphocholine, i.e. POPC, one type of phospholipid mixture - Lipoid H and sterols - cholesterol and b-sitosterol), as well as variation in sterol concentration (5-50%). Fluorescence spectroscopy (anisotropy, r) has shown that DPPC liposomal membranes were more rigid compared to POPC and Lipoid H lipid bilayers. Furthermore, the increment of sterol concentration has caused a decrease in the membrane fluidity in all liposomal samples. On the other hand, phospholipid bilayers containing b-sitosterol has possessed better fluidity in comparison to their cholesterol parallels. Higher permeability of b-sitosterol liposomal membranes, as well as the beneficial effects of phytosterol on human health, favor the use of b-sitosterol in the preparation of liposomes as components of food, pharmaceutical and cosmetic products.
AB  - Prikazana studija daje uvid u promene fluidnosti lipozomalne membrane koje nastaju kao posledica promene strukturnih komponenata lipozoma (dve vrste standardnih fosfolipida - 1,2-dipalmitoil-snglicero-3-fosfoholin, odnosno DPPC i 1-palmitoil-2-oleoil-sn-glicero-3-fosfoholin, odnosno POPC, jedne vrste komercijalne fosfolipidne smeše - Lipoid H i sterola - holesterol i b-sitosterol), kao i promene udela sterola (0-50 mol %). Fluorimetrijska spektrofotometrija (anizotropija, r) pokazala je da su DPPC lipozomalne membrane bile rigidnije u poređenju sa POPC i Lipoid H lipidnim dvoslojima. Pored toga, povećenje koncentracije sterola prouzrokovalo je smanjenje permeabilnosti membrane u svim lipozomima. Sa druge strane, fosfolipidni dvosloji koji su sadržali b-sitosterol pokazali su veću fluidnost u poređenju sa njihovim paralelama sa holesterolom. Bolja permeabilnost b-sitosterol lipozomalnih membrana, kao i blagotvorni uticaji fitosterola na zdravlje ljudi favorizuju primenu b-sitosterola u pripremi lipozoma kao sastavnih delova prehrambenih, farmaceutskih i kozmetičkih proizvoda.
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika
T1  - Fluorescence analysis of liposomal membranes permeability
T1  - Fluorimetrijska analiza permeabilnosti lipozomalnih membrana
EP  - 498
IS  - 4
SP  - 493
VL  - 74
DO  - 10.5937/tehnika1904493J
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Đorđević, Verica and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko and Poklar-Ulrih, Nataša",
year = "2019",
abstract = "The present study provides insight into changes in the liposomal membrane fluidity as a result of variation of structural components (two types of standard phospholipids - 1,2-dipalmitoyl-sn-glycerero3-phosphocholine, i.e. DPPC and 1-palmitoyl-2-oleoylglycerol-3-phosphocholine, i.e. POPC, one type of phospholipid mixture - Lipoid H and sterols - cholesterol and b-sitosterol), as well as variation in sterol concentration (5-50%). Fluorescence spectroscopy (anisotropy, r) has shown that DPPC liposomal membranes were more rigid compared to POPC and Lipoid H lipid bilayers. Furthermore, the increment of sterol concentration has caused a decrease in the membrane fluidity in all liposomal samples. On the other hand, phospholipid bilayers containing b-sitosterol has possessed better fluidity in comparison to their cholesterol parallels. Higher permeability of b-sitosterol liposomal membranes, as well as the beneficial effects of phytosterol on human health, favor the use of b-sitosterol in the preparation of liposomes as components of food, pharmaceutical and cosmetic products., Prikazana studija daje uvid u promene fluidnosti lipozomalne membrane koje nastaju kao posledica promene strukturnih komponenata lipozoma (dve vrste standardnih fosfolipida - 1,2-dipalmitoil-snglicero-3-fosfoholin, odnosno DPPC i 1-palmitoil-2-oleoil-sn-glicero-3-fosfoholin, odnosno POPC, jedne vrste komercijalne fosfolipidne smeše - Lipoid H i sterola - holesterol i b-sitosterol), kao i promene udela sterola (0-50 mol %). Fluorimetrijska spektrofotometrija (anizotropija, r) pokazala je da su DPPC lipozomalne membrane bile rigidnije u poređenju sa POPC i Lipoid H lipidnim dvoslojima. Pored toga, povećenje koncentracije sterola prouzrokovalo je smanjenje permeabilnosti membrane u svim lipozomima. Sa druge strane, fosfolipidni dvosloji koji su sadržali b-sitosterol pokazali su veću fluidnost u poređenju sa njihovim paralelama sa holesterolom. Bolja permeabilnost b-sitosterol lipozomalnih membrana, kao i blagotvorni uticaji fitosterola na zdravlje ljudi favorizuju primenu b-sitosterola u pripremi lipozoma kao sastavnih delova prehrambenih, farmaceutskih i kozmetičkih proizvoda.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika",
title = "Fluorescence analysis of liposomal membranes permeability, Fluorimetrijska analiza permeabilnosti lipozomalnih membrana",
pages = "498-493",
number = "4",
volume = "74",
doi = "10.5937/tehnika1904493J"
}

Jovanović, A., Balanč, B., Đorđević, V., Šavikin, K., Nedović, V., Bugarski, B.,& Poklar-Ulrih, N.. (2019). Fluorescence analysis of liposomal membranes permeability. in Tehnika
Savez inženjera i tehničara Srbije, Beograd., 74(4), 493-498.
https://doi.org/10.5937/tehnika1904493J

Jovanović A, Balanč B, Đorđević V, Šavikin K, Nedović V, Bugarski B, Poklar-Ulrih N. Fluorescence analysis of liposomal membranes permeability. in Tehnika. 2019;74(4):493-498.
doi:10.5937/tehnika1904493J .

Jovanović, Aleksandra, Balanč, Bojana, Đorđević, Verica, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, Poklar-Ulrih, Nataša, "Fluorescence analysis of liposomal membranes permeability" in Tehnika, 74, no. 4 (2019):493-498,
https://doi.org/10.5937/tehnika1904493J . .

TY  - CHAP
AU  - Nikolić, N.C.
AU  - Šavikin, Katarina
AU  - Bigović, D.
AU  - Trifković, Kata T.
AU  - Đorđević, Verica
AU  - Bugarski, Branko
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4201
AB  - In the recent past, bioactive compounds, especially naturally antioxidants, have occupied the main research focus, due to their high biological activity. Plant polyphenols are one of the most potent antioxidants. Encapsulation technique is a promising tool to protect their beneficial properties and to overcome the problems of polyphenols instability and low bioavailability. Micro- and nanohydrogel particles are attractive carriers and have a significant interest because of their unique small size and size-dependent physical properties, which provide many benefits. This chapter is an overview of the current state of the art in encapsulation techniques for polyphenolics with an insight into physicochemical and morphological characteristics of hydrogel-polyphenol systems. Finally, the effect of encapsulation process on biological activity of phenolics will be discussed through the examples available in literature.
T2  - Nanomaterials for Drug Delivery and Therapy
T1  - Potential of encapsulated phytochemicals in hydrogel particles
EP  - 342
SP  - 305
DO  - 10.1016/B978-0-12-816505-8.00009-6
ER  - 

@inbook{
author = "Nikolić, N.C. and Šavikin, Katarina and Bigović, D. and Trifković, Kata T. and Đorđević, Verica and Bugarski, Branko",
year = "2019",
abstract = "In the recent past, bioactive compounds, especially naturally antioxidants, have occupied the main research focus, due to their high biological activity. Plant polyphenols are one of the most potent antioxidants. Encapsulation technique is a promising tool to protect their beneficial properties and to overcome the problems of polyphenols instability and low bioavailability. Micro- and nanohydrogel particles are attractive carriers and have a significant interest because of their unique small size and size-dependent physical properties, which provide many benefits. This chapter is an overview of the current state of the art in encapsulation techniques for polyphenolics with an insight into physicochemical and morphological characteristics of hydrogel-polyphenol systems. Finally, the effect of encapsulation process on biological activity of phenolics will be discussed through the examples available in literature.",
journal = "Nanomaterials for Drug Delivery and Therapy",
booktitle = "Potential of encapsulated phytochemicals in hydrogel particles",
pages = "342-305",
doi = "10.1016/B978-0-12-816505-8.00009-6"
}

Nikolić, N.C., Šavikin, K., Bigović, D., Trifković, K. T., Đorđević, V.,& Bugarski, B.. (2019). Potential of encapsulated phytochemicals in hydrogel particles. in Nanomaterials for Drug Delivery and Therapy, 305-342.
https://doi.org/10.1016/B978-0-12-816505-8.00009-6

Nikolić N, Šavikin K, Bigović D, Trifković KT, Đorđević V, Bugarski B. Potential of encapsulated phytochemicals in hydrogel particles. in Nanomaterials for Drug Delivery and Therapy. 2019;:305-342.
doi:10.1016/B978-0-12-816505-8.00009-6 .

Nikolić, N.C., Šavikin, Katarina, Bigović, D., Trifković, Kata T., Đorđević, Verica, Bugarski, Branko, "Potential of encapsulated phytochemicals in hydrogel particles" in Nanomaterials for Drug Delivery and Therapy (2019):305-342,
https://doi.org/10.1016/B978-0-12-816505-8.00009-6 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Ota, Ajda
AU  - Grabnar, Pegi Ahlin
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Nedović, Viktor
AU  - Poklar-Ulrih, Nataša
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3837
AB  - The influence of different phospholipid types (pure phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC, and one commercial phospholipid mixture, Lipoid H100), sterol types (cholesterol vs. -sitosterol), and various sterol concentrations (5-50mol%) on liposomal membrane fluidity, thermotropic properties, liposome size, zeta potential, and lipid oxidation kinetics using fluorescent lipid probe BODIPY 581/591 C-11 (4,4-difluoro-5-[4-phenyl-1,3-butadienyl]-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid) are investigated. DPPC bilayer is more rigid than POPC and phospholipids mixture membranes. Pure DPPC gives the smallest liposomes, while liposomes of Lipoid H100 have the largest diameter. Both sterols reduce membrane fluidity of all liposomes, increase absolute zeta potential, cause significant changes in particle size, and decrease phase transition temperature (T-m) and enthalpy of DPPC. POPC/-sitosterol liposomes exhibit the most significant lipid oxidation of the lipophilic probe. Along with beneficial effects of phytosterols on human health, better membrane fluidity, more favorable and stabilizing interactions with phospholipids, smaller vesicle size, and enhanced physical stability in comparison to cholesterol are some of the encouraging results for the use of -sitosterol in liposome formulations for potential application in foods, pharmaceutics, and cosmetics.Practical Applications: Adjusting the composition of liposomal membrane (lipid type, sterol type, and concentration) can be used as a tool to control membrane fluidity, permeability, and thermotropic properties, and thus predict release properties, physical, thermal, and oxidative stability. A commercial phospholipid mixture of different natural phospholipids with impurities creates less uniform liposomal membrane that is characterized by higher fluidity in comparison to DPPC. The type of phospholipid has huge influence on MLVs size. -sitosterol, which is a phytosterol with beneficial effects on human health can be used as a replacement for cholesterol in liposomal formulations, but with the following in mind: -sitosterol reduces fluidity of the phospholipid bilayer to a lesser extent than cholesterol, -sitosterol gives smaller MLVs than cholesterol, DPPC/-sitosterol SUVs are bigger than 100nm in diameter (relevant for intravenous administration), MLVs with 30mol% of -sitosterol can be considered as physically stable (unlike those with cholesterol), irrespective to the phospholipid type.
PB  - Wiley, Hoboken
T2  - European Journal of Lipid Science and Technology
T1  - Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics
IS  - 9
VL  - 120
DO  - 10.1002/ejlt.201800039
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Ota, Ajda and Grabnar, Pegi Ahlin and Đorđević, Verica and Šavikin, Katarina and Bugarski, Branko and Nedović, Viktor and Poklar-Ulrih, Nataša",
year = "2018",
abstract = "The influence of different phospholipid types (pure phospholipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, POPC, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC, and one commercial phospholipid mixture, Lipoid H100), sterol types (cholesterol vs. -sitosterol), and various sterol concentrations (5-50mol%) on liposomal membrane fluidity, thermotropic properties, liposome size, zeta potential, and lipid oxidation kinetics using fluorescent lipid probe BODIPY 581/591 C-11 (4,4-difluoro-5-[4-phenyl-1,3-butadienyl]-4-bora-3a,4a-diaza-s-indacene-3-undecanoic acid) are investigated. DPPC bilayer is more rigid than POPC and phospholipids mixture membranes. Pure DPPC gives the smallest liposomes, while liposomes of Lipoid H100 have the largest diameter. Both sterols reduce membrane fluidity of all liposomes, increase absolute zeta potential, cause significant changes in particle size, and decrease phase transition temperature (T-m) and enthalpy of DPPC. POPC/-sitosterol liposomes exhibit the most significant lipid oxidation of the lipophilic probe. Along with beneficial effects of phytosterols on human health, better membrane fluidity, more favorable and stabilizing interactions with phospholipids, smaller vesicle size, and enhanced physical stability in comparison to cholesterol are some of the encouraging results for the use of -sitosterol in liposome formulations for potential application in foods, pharmaceutics, and cosmetics.Practical Applications: Adjusting the composition of liposomal membrane (lipid type, sterol type, and concentration) can be used as a tool to control membrane fluidity, permeability, and thermotropic properties, and thus predict release properties, physical, thermal, and oxidative stability. A commercial phospholipid mixture of different natural phospholipids with impurities creates less uniform liposomal membrane that is characterized by higher fluidity in comparison to DPPC. The type of phospholipid has huge influence on MLVs size. -sitosterol, which is a phytosterol with beneficial effects on human health can be used as a replacement for cholesterol in liposomal formulations, but with the following in mind: -sitosterol reduces fluidity of the phospholipid bilayer to a lesser extent than cholesterol, -sitosterol gives smaller MLVs than cholesterol, DPPC/-sitosterol SUVs are bigger than 100nm in diameter (relevant for intravenous administration), MLVs with 30mol% of -sitosterol can be considered as physically stable (unlike those with cholesterol), irrespective to the phospholipid type.",
publisher = "Wiley, Hoboken",
journal = "European Journal of Lipid Science and Technology",
title = "Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics",
number = "9",
volume = "120",
doi = "10.1002/ejlt.201800039"
}

Jovanović, A., Balanč, B., Ota, A., Grabnar, P. A., Đorđević, V., Šavikin, K., Bugarski, B., Nedović, V.,& Poklar-Ulrih, N.. (2018). Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics. in European Journal of Lipid Science and Technology
Wiley, Hoboken., 120(9).
https://doi.org/10.1002/ejlt.201800039

Jovanović A, Balanč B, Ota A, Grabnar PA, Đorđević V, Šavikin K, Bugarski B, Nedović V, Poklar-Ulrih N. Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics. in European Journal of Lipid Science and Technology. 2018;120(9).
doi:10.1002/ejlt.201800039 .

Jovanović, Aleksandra, Balanč, Bojana, Ota, Ajda, Grabnar, Pegi Ahlin, Đorđević, Verica, Šavikin, Katarina, Bugarski, Branko, Nedović, Viktor, Poklar-Ulrih, Nataša, "Comparative Effects of Cholesterol and β-Sitosterol on the Liposome Membrane Characteristics" in European Journal of Lipid Science and Technology, 120, no. 9 (2018),
https://doi.org/10.1002/ejlt.201800039 . .

TY  - JOUR
AU  - Durdević, Sanja
AU  - Šavikin, Katarina
AU  - Živković, Jelena Č.
AU  - Boehm, Volker
AU  - Stanojković, Tatjana
AU  - Damjanović, Ana
AU  - Petrović, Slobodan
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3961
AB  - Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.
EP  - 232
SP  - 225
VL  - 133
DO  - 10.1016/j.supflu.2017.10.021
ER  - 

@article{
author = "Durdević, Sanja and Šavikin, Katarina and Živković, Jelena Č. and Boehm, Volker and Stanojković, Tatjana and Damjanović, Ana and Petrović, Slobodan",
year = "2018",
abstract = "Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.",
pages = "232-225",
volume = "133",
doi = "10.1016/j.supflu.2017.10.021"
}

Durdević, S., Šavikin, K., Živković, J. Č., Boehm, V., Stanojković, T., Damjanović, A.,& Petrović, S.. (2018). Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 133, 225-232.
https://doi.org/10.1016/j.supflu.2017.10.021

Durdević S, Šavikin K, Živković JČ, Boehm V, Stanojković T, Damjanović A, Petrović S. Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids. 2018;133:225-232.
doi:10.1016/j.supflu.2017.10.021 .

Durdević, Sanja, Šavikin, Katarina, Živković, Jelena Č., Boehm, Volker, Stanojković, Tatjana, Damjanović, Ana, Petrović, Slobodan, "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L." in Journal of Supercritical Fluids, 133 (2018):225-232,
https://doi.org/10.1016/j.supflu.2017.10.021 . .

TY  - JOUR
AU  - Vajić, Una-Jovana
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3880
AB  - Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Phytomedicine
T1  - Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats
EP  - 45
SP  - 39
VL  - 46
DO  - 10.1016/j.phymed.2018.04.037
ER  - 

@article{
author = "Vajić, Una-Jovana and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Ivanov, Milan and Karanović, Danijela and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2018",
abstract = "Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Phytomedicine",
title = "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats",
pages = "45-39",
volume = "46",
doi = "10.1016/j.phymed.2018.04.037"
}

Vajić, U., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Ivanov, M., Karanović, D., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2018). Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 46, 39-45.
https://doi.org/10.1016/j.phymed.2018.04.037

Vajić U, Grujić-Milanović J, Miloradović Z, Jovović Đ, Ivanov M, Karanović D, Šavikin K, Bugarski B, Mihailović-Stanojević N. Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine. 2018;46:39-45.
doi:10.1016/j.phymed.2018.04.037 .

Vajić, Una-Jovana, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Ivanov, Milan, Karanović, Danijela, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats" in Phytomedicine, 46 (2018):39-45,
https://doi.org/10.1016/j.phymed.2018.04.037 . .

TY  - JOUR
AU  - Durdević, Sanja
AU  - Milovanović, Stoja
AU  - Šavikin, Katarina
AU  - Ristić, Mihailo S.
AU  - Menković, Nebojša
AU  - Pljevljakusić, Dejan
AU  - Petrović, Slobodan
AU  - Bogdanović, Aleksandra
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3715
AB  - Microwave radiation was suggested as a pretreatment technique to increase the yield of pomegranate seed oil. Seeds were pretreated at 100, 250 and 600 W during 2 and 6 min and then extracted by supercritical carbon dioxide (scCO(2)) in high pressure unit as well as by n-hexane in Soxhlet apparatus. Even at the lowest microwave pretreatment parameters applied (100 W for 2 min) increased the yield of seed oil obtained by both extraction techniques compared with untreated seeds (from 27.7 to 34.0% and from 21.6 to 25.5% for Soxhlet and scCO(2) extractions, respectively). Maximal oil yield in Soxhlet extraction (36.3%) was obtained with microwave radiation of 600 W for 6 min while for scCO(2) extraction maximal oil yield (27.2%) was with 250 W for 6 min microwave radiation pretreatment. The qualitative and quantitative composition of fatty acids of the obtained oils was determined by gas chromatography/flame ionization detection and gas chromatography/mass spectrometry. Punicic acid was the most abundant fatty acid in pomegranate seed oil ( gt  60%). Microwave pretreatment of seeds showed negligible influence on profile and the amount of fatty acids in obtained extracts, compared with its significant influence on extraction yield. Our results recognize microwave pretreatment as a promising technique for intensification of oil extraction from pomegranate seeds.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Improvement of supercritical CO2 and n-hexane extraction of wild growing pomegranate seed oil by microwave pretreatment
EP  - 27
SP  - 21
VL  - 104
DO  - 10.1016/j.indcrop.2017.04.024
ER  - 

@article{
author = "Durdević, Sanja and Milovanović, Stoja and Šavikin, Katarina and Ristić, Mihailo S. and Menković, Nebojša and Pljevljakusić, Dejan and Petrović, Slobodan and Bogdanović, Aleksandra",
year = "2017",
abstract = "Microwave radiation was suggested as a pretreatment technique to increase the yield of pomegranate seed oil. Seeds were pretreated at 100, 250 and 600 W during 2 and 6 min and then extracted by supercritical carbon dioxide (scCO(2)) in high pressure unit as well as by n-hexane in Soxhlet apparatus. Even at the lowest microwave pretreatment parameters applied (100 W for 2 min) increased the yield of seed oil obtained by both extraction techniques compared with untreated seeds (from 27.7 to 34.0% and from 21.6 to 25.5% for Soxhlet and scCO(2) extractions, respectively). Maximal oil yield in Soxhlet extraction (36.3%) was obtained with microwave radiation of 600 W for 6 min while for scCO(2) extraction maximal oil yield (27.2%) was with 250 W for 6 min microwave radiation pretreatment. The qualitative and quantitative composition of fatty acids of the obtained oils was determined by gas chromatography/flame ionization detection and gas chromatography/mass spectrometry. Punicic acid was the most abundant fatty acid in pomegranate seed oil ( gt  60%). Microwave pretreatment of seeds showed negligible influence on profile and the amount of fatty acids in obtained extracts, compared with its significant influence on extraction yield. Our results recognize microwave pretreatment as a promising technique for intensification of oil extraction from pomegranate seeds.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Improvement of supercritical CO2 and n-hexane extraction of wild growing pomegranate seed oil by microwave pretreatment",
pages = "27-21",
volume = "104",
doi = "10.1016/j.indcrop.2017.04.024"
}

Durdević, S., Milovanović, S., Šavikin, K., Ristić, M. S., Menković, N., Pljevljakusić, D., Petrović, S.,& Bogdanović, A.. (2017). Improvement of supercritical CO2 and n-hexane extraction of wild growing pomegranate seed oil by microwave pretreatment. in Industrial Crops and Products
Elsevier, Amsterdam., 104, 21-27.
https://doi.org/10.1016/j.indcrop.2017.04.024

Durdević S, Milovanović S, Šavikin K, Ristić MS, Menković N, Pljevljakusić D, Petrović S, Bogdanović A. Improvement of supercritical CO2 and n-hexane extraction of wild growing pomegranate seed oil by microwave pretreatment. in Industrial Crops and Products. 2017;104:21-27.
doi:10.1016/j.indcrop.2017.04.024 .

Durdević, Sanja, Milovanović, Stoja, Šavikin, Katarina, Ristić, Mihailo S., Menković, Nebojša, Pljevljakusić, Dejan, Petrović, Slobodan, Bogdanović, Aleksandra, "Improvement of supercritical CO2 and n-hexane extraction of wild growing pomegranate seed oil by microwave pretreatment" in Industrial Crops and Products, 104 (2017):21-27,
https://doi.org/10.1016/j.indcrop.2017.04.024 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3522
AB  - Different conventional (maceration and heat-assisted extraction) and new alternative techniques (ultrasound-assisted and microwave-assisted extractions) have been developed for the extraction of polyphenols compounds from different plant sources. Novel procedures have established with the aim to reduce the extraction time and solvent consumption, as well as to increase polyphenols yield and to improve extract quality. A critical review was conducted to introduce and compare traditional and modern procedures applied for extraction of bioactive polyphenols compounds. This review focuses on the different techniques of polyphenols extraction, discussing their operating conditions, mechanism, choice of particle size and solvent, solid/solvent ratio, extraction time, advantages/disadvantages and effectiveness. Finally, potential application of these extraction procedures in polyphenols isolation is reviewed.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Polyphenols extraction from plant sources
EP  - 49
IS  - 37
SP  - 45
DO  - 10.5937/leksir1737045J
ER  - 

@article{
author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2017",
abstract = "Different conventional (maceration and heat-assisted extraction) and new alternative techniques (ultrasound-assisted and microwave-assisted extractions) have been developed for the extraction of polyphenols compounds from different plant sources. Novel procedures have established with the aim to reduce the extraction time and solvent consumption, as well as to increase polyphenols yield and to improve extract quality. A critical review was conducted to introduce and compare traditional and modern procedures applied for extraction of bioactive polyphenols compounds. This review focuses on the different techniques of polyphenols extraction, discussing their operating conditions, mechanism, choice of particle size and solvent, solid/solvent ratio, extraction time, advantages/disadvantages and effectiveness. Finally, potential application of these extraction procedures in polyphenols isolation is reviewed.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Polyphenols extraction from plant sources",
pages = "49-45",
number = "37",
doi = "10.5937/leksir1737045J"
}

Jovanović, A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2017). Polyphenols extraction from plant sources. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(37), 45-49.
https://doi.org/10.5937/leksir1737045J

Jovanović A, Petrović P, Đorđević V, Zdunić G, Šavikin K, Bugarski B. Polyphenols extraction from plant sources. in Lekovite sirovine. 2017;(37):45-49.
doi:10.5937/leksir1737045J .

Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Polyphenols extraction from plant sources" in Lekovite sirovine, no. 37 (2017):45-49,
https://doi.org/10.5937/leksir1737045J . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Pljevljakusić, Dejan
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3751
AB  - Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE  gt  HAE  gt  maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.
PB  - Elsevier Science Bv, Amsterdam
T2  - Separation and Purification Technology
T1  - Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques
EP  - 380
SP  - 369
VL  - 179
DO  - 10.1016/j.seppur.2017.01.055
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Zdunić, Gordana and Pljevljakusić, Dejan and Šavikin, Katarina and Gođevac, Dejan and Bugarski, Branko",
year = "2017",
abstract = "Despite the fact that Thymus serpyllum is well-known medicinal plant and its chemical profile and biological activity have been investigated, there is no detailed study regarding the influence of different techniques and conditions on the extraction of polyphenolic compounds from Serpylli herba. The aim of this study was optimization of the extraction parameters that improves the efficiency of polyphenols extraction from T. serpyllum: particle size, solid-to-solvent ratio, solvent type and extraction time, by using maceration, heat- and ultrasound-assisted extraction (HAE and UAE). The extraction efficiency was expressed via total polyphenol content (TPC) and total flavonoid content (TFC). The statistical analysis (one-way ANOVA and full factorial design) has revealed that the optimal conditions for achieving the best polyphenols yield were particle size of 0.3 mm, 1:30 solid-to-solvent ratio and 50% ethanol, as environmentally friendly extraction medium, while extraction time has not shown statistically significant influence on polyphenols concentration, in all procedures. Under these conditions, the measured TPC was 26.6 mg GAE/L in maceration, 29.8 mg GAE/L in HAE and 32.7 mg GAE/L in UAE, which was in agreement with the predicted values, while TFC was 143 mg CE/L, 12.4 mg CE/L and 16.7 mg CE/L for maceration, HAE and UAE, respectively. According to total polyphenols yield, the efficiency of the extraction methods for all variables was ranked by significance in the following order: UAE  gt  HAE  gt  maceration, whereas total flavonoids yield was the highest in UAE, although there was no statistically significant difference between maceration and HAE. According to our results, UAE could be selected as the most successful and suitable technique for extraction of bioactive polyphenolic compounds from Serpylli herba. Using LC/MS and HPLC analysis, 9 polyphenolic compounds were identified and quantified: 6,8-Di-C-glucosylapigenin, chlorogenic acid, 6-hydroxyluteolin 7-O-glucoside, caffeic acid, luteolin 7-O-glucuronide, apigenin glucuronide, salvianolic acid g isomer, rosmarinic acid and salvianolic acid I. This study was an initial step in production of polyphenols-rich wild thyme extracts aimed to be used for formulation of foodstuffs and medicines.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Separation and Purification Technology",
title = "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques",
pages = "380-369",
volume = "179",
doi = "10.1016/j.seppur.2017.01.055"
}

Jovanović, A., Đorđević, V., Zdunić, G., Pljevljakusić, D., Šavikin, K., Gođevac, D.,& Bugarski, B.. (2017). Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology
Elsevier Science Bv, Amsterdam., 179, 369-380.
https://doi.org/10.1016/j.seppur.2017.01.055

Jovanović A, Đorđević V, Zdunić G, Pljevljakusić D, Šavikin K, Gođevac D, Bugarski B. Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques. in Separation and Purification Technology. 2017;179:369-380.
doi:10.1016/j.seppur.2017.01.055 .

Jovanović, Aleksandra, Đorđević, Verica, Zdunić, Gordana, Pljevljakusić, Dejan, Šavikin, Katarina, Gođevac, Dejan, Bugarski, Branko, "Optimization of the extraction process of polyphenols from Thymus serpyllum L. herb using maceration, heat- and ultrasound-assisted techniques" in Separation and Purification Technology, 179 (2017):369-380,
https://doi.org/10.1016/j.seppur.2017.01.055 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Pljevljakusić, Dejan
AU  - Bugarski, Branko
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3291
AB  - The present study was designed to establish and optimize a method for extracting natural bioactive compounds from Thymus serpyllum which possess antioxidant, antimicrobial, antispasmodic and stimulant properties. Ultrasound-assisted extraction (UAE) is a welle-stablished method in the processing of plant material, particularly for extraction of bioactive substances such as polyphenols. The influential factors including extraction time (3, 7 and 10 min), solid:solvent ratio (1:10, 1:20 and 1:30) and particle size (0.3, 0.7 and 1.5 mm), have been studied to optimize the extraction process, while using 30% ethanol as an extraction medium and amplitude set to 65%. The yield of UAE was expressed via total phenol content and antioxidant activity of the obtained extracts. The optimum process parameters were found to be: extraction time, 3 min; solid:solvent ratio, 1:30; particle size, 0.3 mm. Under these conditions, the yield of total polyphenols was raised up to 23.03 mg/L GA and the highest antioxidant activity was recorded (10.32 mmol/mg Trolox and IC50 of 3.00 mg/ml).
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity
EP  - 398
IS  - 4
SP  - 391
VL  - 70
DO  - 10.2298/HEMIND150629044J
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Pljevljakusić, Dejan and Bugarski, Branko",
year = "2016",
abstract = "The present study was designed to establish and optimize a method for extracting natural bioactive compounds from Thymus serpyllum which possess antioxidant, antimicrobial, antispasmodic and stimulant properties. Ultrasound-assisted extraction (UAE) is a welle-stablished method in the processing of plant material, particularly for extraction of bioactive substances such as polyphenols. The influential factors including extraction time (3, 7 and 10 min), solid:solvent ratio (1:10, 1:20 and 1:30) and particle size (0.3, 0.7 and 1.5 mm), have been studied to optimize the extraction process, while using 30% ethanol as an extraction medium and amplitude set to 65%. The yield of UAE was expressed via total phenol content and antioxidant activity of the obtained extracts. The optimum process parameters were found to be: extraction time, 3 min; solid:solvent ratio, 1:30; particle size, 0.3 mm. Under these conditions, the yield of total polyphenols was raised up to 23.03 mg/L GA and the highest antioxidant activity was recorded (10.32 mmol/mg Trolox and IC50 of 3.00 mg/ml).",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity",
pages = "398-391",
number = "4",
volume = "70",
doi = "10.2298/HEMIND150629044J"
}

Jovanović, A., Đorđević, V., Zdunić, G., Šavikin, K., Pljevljakusić, D.,& Bugarski, B.. (2016). Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(4), 391-398.
https://doi.org/10.2298/HEMIND150629044J

Jovanović A, Đorđević V, Zdunić G, Šavikin K, Pljevljakusić D, Bugarski B. Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity. in Hemijska industrija. 2016;70(4):391-398.
doi:10.2298/HEMIND150629044J .

Jovanović, Aleksandra, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Pljevljakusić, Dejan, Bugarski, Branko, "Ultrasound-assisted extraction of polyphenols from Thymus serpyllum and its antioxidant activity" in Hemijska industrija, 70, no. 4 (2016):391-398,
https://doi.org/10.2298/HEMIND150629044J . .

TY  - JOUR
AU  - Cujić, Nada
AU  - Trifković, Kata T.
AU  - Bugarski, Branko
AU  - Ibrić, Svetlana
AU  - Pljevljakusić, Dejan
AU  - Šavikin, Katarina
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3246
AB  - Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.
PB  - Elsevier Science Bv, Amsterdam
T2  - Industrial Crops and Products
T1  - Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system
EP  - 131
SP  - 120
VL  - 86
DO  - 10.1016/j.indcrop.2016.03.045
ER  - 

@article{
author = "Cujić, Nada and Trifković, Kata T. and Bugarski, Branko and Ibrić, Svetlana and Pljevljakusić, Dejan and Šavikin, Katarina",
year = "2016",
abstract = "Chokeberry (Aronia melanocarpa) is a rich source of polyphenols with confirmed health benefits. Microencapsulation tehnique is a promising tool for improving its polyphenols functionality, stability and bioavailability. Electrostatic extrusion process was carried out to obtain microbeads with encapsulated chokeberry extract. The effects of the carrier type (alginate of low and medium viscosity), addition of inulin as filler, and the needle diameter (18, 20, 22 gauges) on the morphological characteristics and release properties of the microbeads were studied. Particles obtained with medium viscosity alginate carrier (1.5% w/v), using inulin as filler (5% w/v) and medium needle size (20 gauges) showed the best results in the release studies. Drying process affected the encapsulation efficiency, the amount of encapsulated polyphenols increased from 0.24 mg GAE/g in hydrogel beads to 3.57 mg GAE/g in freeze dried beads, and the release profile of encapsulated extracts was prolonged to 40 min. SEM micrographs confirmed that the addition of inulin as filler improved the final properties of the microbeads, while FTIR analysis showed that the extract was successfully incorporated into the particles. Due to the extended storage and stability, dry microbeads showed the best potential as a delivery system suitable for pharmaceutical or functional food industry.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Industrial Crops and Products",
title = "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system",
pages = "131-120",
volume = "86",
doi = "10.1016/j.indcrop.2016.03.045"
}

Cujić, N., Trifković, K. T., Bugarski, B., Ibrić, S., Pljevljakusić, D.,& Šavikin, K.. (2016). Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products
Elsevier Science Bv, Amsterdam., 86, 120-131.
https://doi.org/10.1016/j.indcrop.2016.03.045

Cujić N, Trifković KT, Bugarski B, Ibrić S, Pljevljakusić D, Šavikin K. Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system. in Industrial Crops and Products. 2016;86:120-131.
doi:10.1016/j.indcrop.2016.03.045 .

Cujić, Nada, Trifković, Kata T., Bugarski, Branko, Ibrić, Svetlana, Pljevljakusić, Dejan, Šavikin, Katarina, "Chokeberry (Aronia melanocarpa L.) extract loaded in alginate and alginate/inulin system" in Industrial Crops and Products, 86 (2016):120-131,
https://doi.org/10.1016/j.indcrop.2016.03.045 . .

TY  - JOUR
AU  - Trifković, Kata T.
AU  - Milašinović, Nikola
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Kalagasidis Krušić, Melina
AU  - Knežević-Jugović, Zorica
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3077
AB  - Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.
PB  - Sage Publications Ltd, London
T2  - Journal of Biomaterials Applications
T1  - Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties
EP  - 631
IS  - 5
SP  - 618
VL  - 30
DO  - 10.1177/0885328215598940
ER  - 

@article{
author = "Trifković, Kata T. and Milašinović, Nikola and Đorđević, Verica and Zdunić, Gordana and Kalagasidis Krušić, Melina and Knežević-Jugović, Zorica and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko",
year = "2015",
abstract = "Chitosan-glutaraldehyde microparticles were produced by emulsion crosslinking method to be used as drug delivery system for polyphenols from Thymus serpyllum L. aqueous extract. The effect of preparation conditions, chitosan concentration (1.5-3% w/v), and glutaraldehyde/chitosan (GA/Ch) mass ratio (0.15-1.20) on water and polyphenols transport properties was investigated. Swelling ratio of dry particles (68-230 mm) in water ranged from 280% to 530%, depending on the formulation. The decrease in swelling was observed with increased GA/Ch mass ratio (i.e. crosslinking degree) at the same chitosan concentration, or with increased chitosan concentration at the same GA/Ch mass ratio. The increase in GA/Ch mass ratio was also manifested by increased particle compactness i.e. decreased size and reduced surface roughness. The sorption capacity for polyphenols seems to be a complex interplay of swelling behaviour and interactions chitosan-glutaraldehyde-polyphenols identified by Fourier transmission infrared analysis. An increase in crystallinity of chitosan was observed upon crosslinking with glutaraldehyde and encapsulation of polyphenols, as observed by X-ray diffraction analysis. The results obtained from release kinetics of selected polyphenolic compounds (caffeic acid, rosmarinic acid, total flavonoids, and total phenol content) showed that polyphenols were released at a lower amount (2-4 times) in water, but more rapidly (45-120 min) in comparison with the release in gastric followed by intestinal simulated fluid (SGF-SIF) (120-240 min). The experimental results of the time-dependent swelling in water and polyphenols release in both, water and SGF-SIF, were analyzed with several mathematical models. The results depicted Fickian diffusion as the water transport mechanism. In the case of polyphenols, only empirical Weibull model could be suggested for describing release kinetics.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Biomaterials Applications",
title = "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties",
pages = "631-618",
number = "5",
volume = "30",
doi = "10.1177/0885328215598940"
}

Trifković, K. T., Milašinović, N., Đorđević, V., Zdunić, G., Kalagasidis Krušić, M., Knežević-Jugović, Z., Šavikin, K., Nedović, V.,& Bugarski, B.. (2015). Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of Biomaterials Applications
Sage Publications Ltd, London., 30(5), 618-631.
https://doi.org/10.1177/0885328215598940

Trifković KT, Milašinović N, Đorđević V, Zdunić G, Kalagasidis Krušić M, Knežević-Jugović Z, Šavikin K, Nedović V, Bugarski B. Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties. in Journal of Biomaterials Applications. 2015;30(5):618-631.
doi:10.1177/0885328215598940 .

Trifković, Kata T., Milašinović, Nikola, Đorđević, Verica, Zdunić, Gordana, Kalagasidis Krušić, Melina, Knežević-Jugović, Zorica, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "Chitosan crosslinked microparticles with encapsulated polyphenols: Water sorption and release properties" in Journal of Biomaterials Applications, 30, no. 5 (2015):618-631,
https://doi.org/10.1177/0885328215598940 . .

TY  - JOUR
AU  - Vajić, Una-Jovana
AU  - Grujić-Milanović, Jelica
AU  - Živković, Jelena Č.
AU  - Šavikin, Katarina
AU  - Gođevac, Dejan
AU  - Miloradović, Zoran
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3145
AB  - A full three level factorial design was implemented for optimization of extraction parameters in order to maximize total phenolic (TP) yield from stinging nettle leaf. Factors considered were percentage of methanol in solvent (X-1 : 50-100% methanol) and extraction time (X-2: 30-90 min), and maceration was used as extraction method. A second-order polynomial model was applied for fitting experimental data and predicting the response, and this mathematical model produced a satisfactory fit (R-2 = 0.993,p lt 0.01). The optimal extraction conditions were 54% aqueous methanol and 38 min extraction time, while maximal theoretical TP yield was 8.9 mg GAE/g DW. Solvent composition significantly affected extraction efficiency causing decrease of TP yield with increase of methanol percentage. On the other hand, extraction time did not influence significantly efficiency of extraction. Using LC/MS and HPLC analysis we detected and quantified three most abundant phenolic compounds: 2-O-caffeoyl malic acid, chlorogenic acid, and rutin. Comparison between maceration and ultrasound-assisted extraction (UAE) obtained extracts based on TP content as well as antiradical activity and HPLC results, showed that UAE have a better extraction capability affecting yield and time of extraction. Of all tested extracts, 54% aqueous methanolic extracts obtained with UAE and 38 min extraction time had the highest TP content.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology
EP  - 917
SP  - 912
VL  - 74
DO  - 10.1016/j.indcrop.2015.06.032
ER  - 

@article{
author = "Vajić, Una-Jovana and Grujić-Milanović, Jelica and Živković, Jelena Č. and Šavikin, Katarina and Gođevac, Dejan and Miloradović, Zoran and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2015",
abstract = "A full three level factorial design was implemented for optimization of extraction parameters in order to maximize total phenolic (TP) yield from stinging nettle leaf. Factors considered were percentage of methanol in solvent (X-1 : 50-100% methanol) and extraction time (X-2: 30-90 min), and maceration was used as extraction method. A second-order polynomial model was applied for fitting experimental data and predicting the response, and this mathematical model produced a satisfactory fit (R-2 = 0.993,p lt 0.01). The optimal extraction conditions were 54% aqueous methanol and 38 min extraction time, while maximal theoretical TP yield was 8.9 mg GAE/g DW. Solvent composition significantly affected extraction efficiency causing decrease of TP yield with increase of methanol percentage. On the other hand, extraction time did not influence significantly efficiency of extraction. Using LC/MS and HPLC analysis we detected and quantified three most abundant phenolic compounds: 2-O-caffeoyl malic acid, chlorogenic acid, and rutin. Comparison between maceration and ultrasound-assisted extraction (UAE) obtained extracts based on TP content as well as antiradical activity and HPLC results, showed that UAE have a better extraction capability affecting yield and time of extraction. Of all tested extracts, 54% aqueous methanolic extracts obtained with UAE and 38 min extraction time had the highest TP content.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology",
pages = "917-912",
volume = "74",
doi = "10.1016/j.indcrop.2015.06.032"
}

Vajić, U., Grujić-Milanović, J., Živković, J. Č., Šavikin, K., Gođevac, D., Miloradović, Z., Bugarski, B.,& Mihailović-Stanojević, N.. (2015). Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. in Industrial Crops and Products
Elsevier, Amsterdam., 74, 912-917.
https://doi.org/10.1016/j.indcrop.2015.06.032

Vajić U, Grujić-Milanović J, Živković JČ, Šavikin K, Gođevac D, Miloradović Z, Bugarski B, Mihailović-Stanojević N. Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. in Industrial Crops and Products. 2015;74:912-917.
doi:10.1016/j.indcrop.2015.06.032 .

Vajić, Una-Jovana, Grujić-Milanović, Jelica, Živković, Jelena Č., Šavikin, Katarina, Gođevac, Dejan, Miloradović, Zoran, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology" in Industrial Crops and Products, 74 (2015):912-917,
https://doi.org/10.1016/j.indcrop.2015.06.032 . .

TY  - JOUR
AU  - Čančarević, Aleksandra
AU  - Bugarski, Branko
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2297
AB  - The Lamiaceae family, which contains about 200 genus and 3000 species, has a cosmopolitan distribution. Plants of this family are rich in essential oils and phenol compounds. Thymus vulgaris (Thyme) is perennial, subshrub plant which belongs to family of Labiates. Calyx and corolla are covered by dense gleaming glands that eradiate essential oil. Leaves or herba, and essential oils are used. Thymus serpyllum (Wild Thyme) is perennial, herbaceous plant. Leaves are evergreen, small and oval. Commitee on Herbal Medicinal Products (HMPC) classifies thyme herba and essential oil as a traditional herbal medicine used as expectorant in cough associated with cold. It is used in folk medicine to treat many diseases, including respiratory disease (cough pertussis, bronchitis and asthma), and has analgesic properties. Wild Thyme is approved for use in treatment of cough and bronchitis. It is unproven to treat kidney and bladder disorders. It is also used internally for dysmenorrheal, colic-like pain, and whooping cough. Thymol and carvacrol from essential oils of Thyme and Wild Thyme inhibit lipid peroxidation and manifest strong antimicrobial activity against different kinds of microorganisms. All the aromatic and medicinal plants available worldwide have been used for their preservative and medicinal values. Polyphenolic phytochemicals in traditionally used medicinal plants act as powerful antioxidants, which aroused an increasing interest in their application in functional food development. Recently, there has been growing considerable interest in extracts and essential oils of aromatic plants for the development of alternative food additives, in order to prevent the growth of food-borne pathogens or to delay the onset of food spoilage.
AB  - Familija Lamiaceae (Usnatice) obuhvata veliki broj kosmopolitskih vrsta, među kojima i vrste T. vulgaris (timijan) i T. serpyllum (majčina dušica), koje karakteriše prisustvo etarskih ulja i fenolnih jedinjenja. Timijan predstavlja višegodišnju polužbunastu biljku. Čašicu i krunicu gusto pokrivaju trihome u kojima se sintetišu i skladište etarska ulja. Koristi se list ili zeljasti nadzemni deo biljke u cvetu i etarsko ulje. Majčina dušica je višegodišnja zeljasta biljka. Cvetne grane su ravnomerno prekrivene žlezdanim trihomama. Koristi se nadzemni deo biljke u cvetu. Herbal Medicinal Product Committee (HMPC) klasifikuje herbu i etarsko ulje timijana kao tradicionalni biljni lek koji se koristi kao ekspektorans kod kašlja udruženog sa prehladom. Potvrđena primena majčine dušice je u tretmanu kašlja, bronhitisa i upalnih procesa gornjih disajnih puteva. Snažnu antioksidativnu aktivnost timijan duguje visokoj koncentraciji polifenolnih kiselina i flavonoidima. Timol i karvakrol prisutni u etarskom ulju majčine dušice inhibiraju lipidnu peroksidaciju i ispoljavaju antimikrobno dejstvo na različite vrste mikroorganizama. Dugogodišnja tradicionalna primena aromatičnih i lekovitih biljaka, pritisak potrošača da se toksični i kancerogeni sintetski aditivi zamene prirodnim supstancama i pojava multirezistentnih sojeva mikroorganizama povećavaju interes za istraživanjem datih biljnih vrsta, što bi omogućilo njihovu širu primenu u medicini, prehrambenoj, farmaceutskoj i kozmetičkoj industriji.
PB  - Institute for Medicinal Plants Research "Dr. Josif Pancic"
T2  - Lekovite sirovine
T1  - Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum
T1  - Biološka aktivnost vrsta Thymus vulgaris i Thymus serpyllum i njihovo korišćenje u etnomedicini
EP  - 17
IS  - 33
SP  - 3
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2297
ER  - 

@article{
author = "Čančarević, Aleksandra and Bugarski, Branko and Šavikin, Katarina and Zdunić, Gordana",
year = "2013",
abstract = "The Lamiaceae family, which contains about 200 genus and 3000 species, has a cosmopolitan distribution. Plants of this family are rich in essential oils and phenol compounds. Thymus vulgaris (Thyme) is perennial, subshrub plant which belongs to family of Labiates. Calyx and corolla are covered by dense gleaming glands that eradiate essential oil. Leaves or herba, and essential oils are used. Thymus serpyllum (Wild Thyme) is perennial, herbaceous plant. Leaves are evergreen, small and oval. Commitee on Herbal Medicinal Products (HMPC) classifies thyme herba and essential oil as a traditional herbal medicine used as expectorant in cough associated with cold. It is used in folk medicine to treat many diseases, including respiratory disease (cough pertussis, bronchitis and asthma), and has analgesic properties. Wild Thyme is approved for use in treatment of cough and bronchitis. It is unproven to treat kidney and bladder disorders. It is also used internally for dysmenorrheal, colic-like pain, and whooping cough. Thymol and carvacrol from essential oils of Thyme and Wild Thyme inhibit lipid peroxidation and manifest strong antimicrobial activity against different kinds of microorganisms. All the aromatic and medicinal plants available worldwide have been used for their preservative and medicinal values. Polyphenolic phytochemicals in traditionally used medicinal plants act as powerful antioxidants, which aroused an increasing interest in their application in functional food development. Recently, there has been growing considerable interest in extracts and essential oils of aromatic plants for the development of alternative food additives, in order to prevent the growth of food-borne pathogens or to delay the onset of food spoilage., Familija Lamiaceae (Usnatice) obuhvata veliki broj kosmopolitskih vrsta, među kojima i vrste T. vulgaris (timijan) i T. serpyllum (majčina dušica), koje karakteriše prisustvo etarskih ulja i fenolnih jedinjenja. Timijan predstavlja višegodišnju polužbunastu biljku. Čašicu i krunicu gusto pokrivaju trihome u kojima se sintetišu i skladište etarska ulja. Koristi se list ili zeljasti nadzemni deo biljke u cvetu i etarsko ulje. Majčina dušica je višegodišnja zeljasta biljka. Cvetne grane su ravnomerno prekrivene žlezdanim trihomama. Koristi se nadzemni deo biljke u cvetu. Herbal Medicinal Product Committee (HMPC) klasifikuje herbu i etarsko ulje timijana kao tradicionalni biljni lek koji se koristi kao ekspektorans kod kašlja udruženog sa prehladom. Potvrđena primena majčine dušice je u tretmanu kašlja, bronhitisa i upalnih procesa gornjih disajnih puteva. Snažnu antioksidativnu aktivnost timijan duguje visokoj koncentraciji polifenolnih kiselina i flavonoidima. Timol i karvakrol prisutni u etarskom ulju majčine dušice inhibiraju lipidnu peroksidaciju i ispoljavaju antimikrobno dejstvo na različite vrste mikroorganizama. Dugogodišnja tradicionalna primena aromatičnih i lekovitih biljaka, pritisak potrošača da se toksični i kancerogeni sintetski aditivi zamene prirodnim supstancama i pojava multirezistentnih sojeva mikroorganizama povećavaju interes za istraživanjem datih biljnih vrsta, što bi omogućilo njihovu širu primenu u medicini, prehrambenoj, farmaceutskoj i kozmetičkoj industriji.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum, Biološka aktivnost vrsta Thymus vulgaris i Thymus serpyllum i njihovo korišćenje u etnomedicini",
pages = "17-3",
number = "33",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2297"
}

Čančarević, A., Bugarski, B., Šavikin, K.,& Zdunić, G.. (2013). Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum. in Lekovite sirovine
Institute for Medicinal Plants Research "Dr. Josif Pancic".(33), 3-17.
https://hdl.handle.net/21.15107/rcub_technorep_2297

Čančarević A, Bugarski B, Šavikin K, Zdunić G. Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum. in Lekovite sirovine. 2013;(33):3-17.
https://hdl.handle.net/21.15107/rcub_technorep_2297 .

Čančarević, Aleksandra, Bugarski, Branko, Šavikin, Katarina, Zdunić, Gordana, "Biological activity and ethnomedicinal use of Thymus vulgaris and Thymus serpyllum" in Lekovite sirovine, no. 33 (2013):3-17,
https://hdl.handle.net/21.15107/rcub_technorep_2297 .