TY  - JOUR
AU  - Ilić-Stojanović, Snežana
AU  - Nikolić, Ljubiša
AU  - Nikolić, Vesna
AU  - Petrović, Slobodan
AU  - Oro, Violeta
AU  - Mitić, Žarko
AU  - Najman, Stevo
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4899
AB  - In this study, poly(N-isopropylacrylamide-co-2-hydroxypropyl methacrylate) hydrogels were synthesized using free radical initiated copolymerization method. Four hydrogels with different cross-linker concentrations were prepared. Semi-crystalline, cross-linked copolymer networks were confirmed by FTIR, SEM and XRD analysis. Variation of swelling behaviour was monitored gravimetrically and thermo-responsiveness has been noticed. An application of synthesized thermo-responsive hydrogels as carriers for the modulated release of anti-inflammatory model drug was investigated. Moreover, naproxen loading into these hydrogels was also determined using FTIR, SEM and XRD techniques and release was analyzed using HPLC method at simulated physiological conditions. Swelling kinetic and mechanism of water transport, as well as diffusion of naproxen through the hydrogels were analyzed. Thus, the aim of this work was to study various compositions of obtained hydrogels and their possibility of application as a thermo-responsive carrier for prolonged naproxen release in order to evaluate as a potential candidate for drug carrier in future pharmaceutical applications.
T2  - Pharmaceutics
T1  - Semi-Crystalline Copolymer Hydrogels as Smart Drug Carriers: In Vitro Thermo-Responsive Naproxen Release Study
IS  - 2
VL  - 13
DO  - 10.3390/pharmaceutics13020158
ER  - 

@article{
author = "Ilić-Stojanović, Snežana and Nikolić, Ljubiša and Nikolić, Vesna and Petrović, Slobodan and Oro, Violeta and Mitić, Žarko and Najman, Stevo",
year = "2021",
abstract = "In this study, poly(N-isopropylacrylamide-co-2-hydroxypropyl methacrylate) hydrogels were synthesized using free radical initiated copolymerization method. Four hydrogels with different cross-linker concentrations were prepared. Semi-crystalline, cross-linked copolymer networks were confirmed by FTIR, SEM and XRD analysis. Variation of swelling behaviour was monitored gravimetrically and thermo-responsiveness has been noticed. An application of synthesized thermo-responsive hydrogels as carriers for the modulated release of anti-inflammatory model drug was investigated. Moreover, naproxen loading into these hydrogels was also determined using FTIR, SEM and XRD techniques and release was analyzed using HPLC method at simulated physiological conditions. Swelling kinetic and mechanism of water transport, as well as diffusion of naproxen through the hydrogels were analyzed. Thus, the aim of this work was to study various compositions of obtained hydrogels and their possibility of application as a thermo-responsive carrier for prolonged naproxen release in order to evaluate as a potential candidate for drug carrier in future pharmaceutical applications.",
journal = "Pharmaceutics",
title = "Semi-Crystalline Copolymer Hydrogels as Smart Drug Carriers: In Vitro Thermo-Responsive Naproxen Release Study",
number = "2",
volume = "13",
doi = "10.3390/pharmaceutics13020158"
}

Ilić-Stojanović, S., Nikolić, L., Nikolić, V., Petrović, S., Oro, V., Mitić, Ž.,& Najman, S.. (2021). Semi-Crystalline Copolymer Hydrogels as Smart Drug Carriers: In Vitro Thermo-Responsive Naproxen Release Study. in Pharmaceutics, 13(2).
https://doi.org/10.3390/pharmaceutics13020158

Ilić-Stojanović S, Nikolić L, Nikolić V, Petrović S, Oro V, Mitić Ž, Najman S. Semi-Crystalline Copolymer Hydrogels as Smart Drug Carriers: In Vitro Thermo-Responsive Naproxen Release Study. in Pharmaceutics. 2021;13(2).
doi:10.3390/pharmaceutics13020158 .

Ilić-Stojanović, Snežana, Nikolić, Ljubiša, Nikolić, Vesna, Petrović, Slobodan, Oro, Violeta, Mitić, Žarko, Najman, Stevo, "Semi-Crystalline Copolymer Hydrogels as Smart Drug Carriers: In Vitro Thermo-Responsive Naproxen Release Study" in Pharmaceutics, 13, no. 2 (2021),
https://doi.org/10.3390/pharmaceutics13020158 . .

TY  - JOUR
AU  - Krezović, Bojana D.
AU  - Miljković, Miona
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Filipović, Jovanka
AU  - Tomić, Simonida
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3709
AB  - The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.
PB  - Inst Chemical Engineers, Rugby
T2  - Chemical Engineering Research & Design
T1  - Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels
EP  - 380
SP  - 368
VL  - 121
DO  - 10.1016/j.cherd.2017.03.030
ER  - 

@article{
author = "Krezović, Bojana D. and Miljković, Miona and Stojanović, Sanja T. and Najman, Stevo and Filipović, Jovanka and Tomić, Simonida",
year = "2017",
abstract = "The aim of this work was to synthesize a series of semi-interpenetrating polymer networks (semi-IPNs) of 2-hydroxyethyl acrylate and itaconic acid, in the presence of poly(N-vinylpyrrolidone). Samples were synthesized by free radical copolymerization with constant PVP and variable IA content and characterized for structural, morphological, thermal, swelling, drug release, antibacterial and cytotoxic properties. The chemical structure of samples was confirmed by Fourier-transform infrared spectroscopy (FTIR). Scanning electron microscopy (SEM) was used to examine morphology of samples and glass transition temperatures were determined by differential scanning calorimetry (DCS). The pH and temperature sensitivity was confirmed by measuring the dependence of the degree of swelling on pH and temperature. All samples show volume phase transition temperature (VPTT) around 47 degrees C. Drug release profiles were investigated using vitamin B-3 as a model drug. The vitamin B-3 transport mechanism was studied by fitting experimental data to five different model equations and calculating the corresponding parameters. It was also observed that IA content has a marked influence on the release profiles of vitamin B-3, so the rate of drug release can be designed by changing the HEA/IA ratio. Cell viability, performed using MTT test, was high for all samples and all concentration of extract used. The antibacterial activity of hydrogels was determined against Pseudomonas aeruginosa. It turns out that IA content and time of exposure have an influence on the antibacterial potential. All samples showed satisfied cell viability. Due to these characteristics, P(HEA/IA)/PVP semi-IPNs represent interesting biomaterials for biomedical applications.",
publisher = "Inst Chemical Engineers, Rugby",
journal = "Chemical Engineering Research & Design",
title = "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels",
pages = "380-368",
volume = "121",
doi = "10.1016/j.cherd.2017.03.030"
}

Krezović, B. D., Miljković, M., Stojanović, S. T., Najman, S., Filipović, J.,& Tomić, S.. (2017). Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design
Inst Chemical Engineers, Rugby., 121, 368-380.
https://doi.org/10.1016/j.cherd.2017.03.030

Krezović BD, Miljković M, Stojanović ST, Najman S, Filipović J, Tomić S. Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels. in Chemical Engineering Research & Design. 2017;121:368-380.
doi:10.1016/j.cherd.2017.03.030 .

Krezović, Bojana D., Miljković, Miona, Stojanović, Sanja T., Najman, Stevo, Filipović, Jovanka, Tomić, Simonida, "Structural, thermal, mechanical, swelling, drug release, antibacterial and cytotoxic properties of P(HEMA)/PVP semi-IPN hydrogels" in Chemical Engineering Research & Design, 121 (2017):368-380,
https://doi.org/10.1016/j.cherd.2017.03.030 . .

TY  - JOUR
AU  - Vuković, Jovana
AU  - Babić, Marija
AU  - Antić, Katarina
AU  - Filipović, Jovanka M.
AU  - Stojanović, Sanja T.
AU  - Najman, Stevo
AU  - Tomić, Simonida
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3352
AB  - In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.
PB  - Elsevier Science Sa, Lausanne
T2  - Materials Chemistry and Physics
T1  - In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management
EP  - 163
SP  - 158
VL  - 175
DO  - 10.1016/j.matchemphys.2016.03.009
ER  - 

@article{
author = "Vuković, Jovana and Babić, Marija and Antić, Katarina and Filipović, Jovanka M. and Stojanović, Sanja T. and Najman, Stevo and Tomić, Simonida",
year = "2016",
abstract = "In order to evaluate biological response and potential toxicity prior to clinical use as wound dressing materials, three series of hydrogels, based on 2-hydroxyethyl acrylate (HEA) and itaconic acid (IA), unloaded, with incorporated copper(II) ions and reduced copper, were subjected to cytotoxicity testing on L929 cell line (mouse fibroblasts). MTT test revealed proper fibroblast growth in the presence of tested hydrogels, with an absence of any acute toxic effects. Mechanical properties of all three hydrogel series were studied by dynamic mechanical analysis (DMA) and obtained results indicated dominant elastic over viscous behaviour, which supports their application in wound management. The temperature sensitive behaviour of the hydrogels was detected in swelling study conducted in wide range of temperature values 25-50 degrees C, in buffer pH 7.40. The hydrogels exhibited excellent microbial barrier characteristics against Pseudomonas aeruginosa, reducing the risk of bacterial infection in wound bed.",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Materials Chemistry and Physics",
title = "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management",
pages = "163-158",
volume = "175",
doi = "10.1016/j.matchemphys.2016.03.009"
}

Vuković, J., Babić, M., Antić, K., Filipović, J. M., Stojanović, S. T., Najman, S.,& Tomić, S.. (2016). In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics
Elsevier Science Sa, Lausanne., 175, 158-163.
https://doi.org/10.1016/j.matchemphys.2016.03.009

Vuković J, Babić M, Antić K, Filipović JM, Stojanović ST, Najman S, Tomić S. In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management. in Materials Chemistry and Physics. 2016;175:158-163.
doi:10.1016/j.matchemphys.2016.03.009 .

Vuković, Jovana, Babić, Marija, Antić, Katarina, Filipović, Jovanka M., Stojanović, Sanja T., Najman, Stevo, Tomić, Simonida, "In vitro cytotoxicity assessment of intelligent acrylate based hydrogels with incorporated copper in wound management" in Materials Chemistry and Physics, 175 (2016):158-163,
https://doi.org/10.1016/j.matchemphys.2016.03.009 . .

TY  - JOUR
AU  - Takić-Miladinov, Dijana
AU  - Tomić, Simonida
AU  - Stojanović, Sanja T.
AU  - Najdanović, Jelena
AU  - Filipović, Jovanka
AU  - Trajanović, Miroslav
AU  - Najman, Stevo
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3307
AB  - In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.
PB  - University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos
T2  - Materials Research-Ibero-American Journal of Materials
T1  - Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid
EP  - 1079
IS  - 5
SP  - 1070
VL  - 19
DO  - 10.1590/1980-5373-MR-2016-0222
ER  - 

@article{
author = "Takić-Miladinov, Dijana and Tomić, Simonida and Stojanović, Sanja T. and Najdanović, Jelena and Filipović, Jovanka and Trajanović, Miroslav and Najman, Stevo",
year = "2016",
abstract = "In this study we prepared hydrogels based on 2-hydroxyethyl methacrylate (HEMA): PHEMA homopolymer and two terpolymers of HEMA, itaconic acid (IA) and two poly(alkylene glycol) (meth) acrylates (PAGM): poly(ethylene glycol)(6) acrylate (P(HEMA/IA/PAGM1)) and poly(propylene glycol)(5) methacrylate (P(HEMA/IA/PAGM2)). Hydrogels were synthesized by gamma-irradiated radical polymerization and subjected to swelling measurements and genotoxicity evaluation. Swelling studies confirmed that these hydrogels deserve consideration as biomaterials due to their ability to swell in phosphate buffer but maintaining physical integrity for a prolonged contact time after equilibrium state has been reached. Comet assay showed certain genotoxic effect following cell exposure to extracts of hydrogels, which was dependent on the concentration of extracts, chemical composition of hydrogels and the degree of crosslinking. The influence of concentration on genotoxicity was the most pronounced. The synthesis of these novel HEMA-based hydrogels should be optimized so as to reduce their toxicity and enable the use in clinical practice.",
publisher = "University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos",
journal = "Materials Research-Ibero-American Journal of Materials",
title = "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid",
pages = "1079-1070",
number = "5",
volume = "19",
doi = "10.1590/1980-5373-MR-2016-0222"
}

Takić-Miladinov, D., Tomić, S., Stojanović, S. T., Najdanović, J., Filipović, J., Trajanović, M.,& Najman, S.. (2016). Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials
University of Fed Sao Carlos, Dept Engenharia Materials, Sao Carlos., 19(5), 1070-1079.
https://doi.org/10.1590/1980-5373-MR-2016-0222

Takić-Miladinov D, Tomić S, Stojanović ST, Najdanović J, Filipović J, Trajanović M, Najman S. Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid. in Materials Research-Ibero-American Journal of Materials. 2016;19(5):1070-1079.
doi:10.1590/1980-5373-MR-2016-0222 .

Takić-Miladinov, Dijana, Tomić, Simonida, Stojanović, Sanja T., Najdanović, Jelena, Filipović, Jovanka, Trajanović, Miroslav, Najman, Stevo, "Synthesis, Swelling Properties and Evaluation of Genotoxicity of Hydrogels Based on (Meth)acrylates and Itaconic Acid" in Materials Research-Ibero-American Journal of Materials, 19, no. 5 (2016):1070-1079,
https://doi.org/10.1590/1980-5373-MR-2016-0222 . .

TY  - JOUR
AU  - Tomić, Simonida
AU  - Dimitrijević, Suzana
AU  - Marinković, Aleksandar
AU  - Najman, Stevo
AU  - Filipović, Jovanka M.
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1465
AB  - In the present study poly(2-hydroxyethyl methacrylate-co-itaconic acid) (P(HEMA/IA)) hydrogels were synthesized by free-radical copolymerization of 2-hydroxyethyl methacrylate and itaconic acid in order to evaluate as controlled release drug delivery system. All polymerizations were performed in a mixture of water/ethanol with ethylene glycol dimethacrylate, as crosslinking agent, potassium persulfate, as initiator, and N, N, N', N'-tetramethylethylene diamine, as activator. The samples were characterized by FTIR and SEM. Swelling kinetics and antibiotic release studies were performed using gravimetry and UV spectrophotometry, respectively. The antibacterial activity studies were performed bearing in mind that infections are the most common cause of biomaterial implant failure and represent a constant menace to the application of medical implants. The hemocompatibility testing was performed as imperative for medical devices intended for direct or indirect blood exposure. In accordance with the results of antibacterial assessment on gels loaded with antibiotics and hemolytic activity testing these gels exerted good bacterial growth inhibition and favorable hemolytic activity. The release profiles of antibiotics, evaluated in vitro conditions, were correspondent for antibacterial therapeutics. Therefore, it was concluded that (P(HEMA/IA)) gels could be propound for the potential application as drug delivery systems for the controlled release of antibiotics.
PB  - Springer, New York
T2  - Polymer Bulletin
T1  - Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid) copolymeric hydrogels
EP  - 851
IS  - 6
SP  - 837
VL  - 63
DO  - 10.1007/s00289-009-0123-2
ER  - 

@article{
author = "Tomić, Simonida and Dimitrijević, Suzana and Marinković, Aleksandar and Najman, Stevo and Filipović, Jovanka M.",
year = "2009",
abstract = "In the present study poly(2-hydroxyethyl methacrylate-co-itaconic acid) (P(HEMA/IA)) hydrogels were synthesized by free-radical copolymerization of 2-hydroxyethyl methacrylate and itaconic acid in order to evaluate as controlled release drug delivery system. All polymerizations were performed in a mixture of water/ethanol with ethylene glycol dimethacrylate, as crosslinking agent, potassium persulfate, as initiator, and N, N, N', N'-tetramethylethylene diamine, as activator. The samples were characterized by FTIR and SEM. Swelling kinetics and antibiotic release studies were performed using gravimetry and UV spectrophotometry, respectively. The antibacterial activity studies were performed bearing in mind that infections are the most common cause of biomaterial implant failure and represent a constant menace to the application of medical implants. The hemocompatibility testing was performed as imperative for medical devices intended for direct or indirect blood exposure. In accordance with the results of antibacterial assessment on gels loaded with antibiotics and hemolytic activity testing these gels exerted good bacterial growth inhibition and favorable hemolytic activity. The release profiles of antibiotics, evaluated in vitro conditions, were correspondent for antibacterial therapeutics. Therefore, it was concluded that (P(HEMA/IA)) gels could be propound for the potential application as drug delivery systems for the controlled release of antibiotics.",
publisher = "Springer, New York",
journal = "Polymer Bulletin",
title = "Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid) copolymeric hydrogels",
pages = "851-837",
number = "6",
volume = "63",
doi = "10.1007/s00289-009-0123-2"
}

Tomić, S., Dimitrijević, S., Marinković, A., Najman, S.,& Filipović, J. M.. (2009). Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid) copolymeric hydrogels. in Polymer Bulletin
Springer, New York., 63(6), 837-851.
https://doi.org/10.1007/s00289-009-0123-2

Tomić S, Dimitrijević S, Marinković A, Najman S, Filipović JM. Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid) copolymeric hydrogels. in Polymer Bulletin. 2009;63(6):837-851.
doi:10.1007/s00289-009-0123-2 .

Tomić, Simonida, Dimitrijević, Suzana, Marinković, Aleksandar, Najman, Stevo, Filipović, Jovanka M., "Synthesis and characterization of poly(2-hydroxyethyl methacrylate/itaconic acid) copolymeric hydrogels" in Polymer Bulletin, 63, no. 6 (2009):837-851,
https://doi.org/10.1007/s00289-009-0123-2 . .

TY  - CONF
AU  - Milojević, Mirjana
AU  - Najman, Stevo
AU  - Tomić, Simonida
AU  - Đorđević, Ljubiša
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7369
AB  - In this study hemolytic activity of hydrogels different chemical construction and
synthesized under different conditions was examined. The hydrogels are synthesized by
radiation and chemical polymerization of 2-hydroxyethyl methacrylate (HEMA),
copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with
poly (alkylene glycol) (met) acrylates - Bisomers. In this purpose we used hemolytic test on rat
erythrocytes. Materials with the same area show different hemolytic activity. According to given
hemolysis, all of tested materials can be considered nonhemolytic.
PB  - Belgrade : Institute of Technical Sciences of SASA
C3  - Programme and the Book of Abstracts / Ninth Annual Conference of the Yugoslav Materials Research Society YUCOMAT 2007, Herceg Novi, September 10-14, 2007
T1  - Effect of hydrogels based on 2-hydroxyethyl methacrylates to erythrocyte resistance to hemolysis
SP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7369
ER  - 

@conference{
author = "Milojević, Mirjana and Najman, Stevo and Tomić, Simonida and Đorđević, Ljubiša",
year = "2007",
abstract = "In this study hemolytic activity of hydrogels different chemical construction and
synthesized under different conditions was examined. The hydrogels are synthesized by
radiation and chemical polymerization of 2-hydroxyethyl methacrylate (HEMA),
copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with
poly (alkylene glycol) (met) acrylates - Bisomers. In this purpose we used hemolytic test on rat
erythrocytes. Materials with the same area show different hemolytic activity. According to given
hemolysis, all of tested materials can be considered nonhemolytic.",
publisher = "Belgrade : Institute of Technical Sciences of SASA",
journal = "Programme and the Book of Abstracts / Ninth Annual Conference of the Yugoslav Materials Research Society YUCOMAT 2007, Herceg Novi, September 10-14, 2007",
title = "Effect of hydrogels based on 2-hydroxyethyl methacrylates to erythrocyte resistance to hemolysis",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7369"
}

Milojević, M., Najman, S., Tomić, S.,& Đorđević, L.. (2007). Effect of hydrogels based on 2-hydroxyethyl methacrylates to erythrocyte resistance to hemolysis. in Programme and the Book of Abstracts / Ninth Annual Conference of the Yugoslav Materials Research Society YUCOMAT 2007, Herceg Novi, September 10-14, 2007
Belgrade : Institute of Technical Sciences of SASA., 180.
https://hdl.handle.net/21.15107/rcub_technorep_7369

Milojević M, Najman S, Tomić S, Đorđević L. Effect of hydrogels based on 2-hydroxyethyl methacrylates to erythrocyte resistance to hemolysis. in Programme and the Book of Abstracts / Ninth Annual Conference of the Yugoslav Materials Research Society YUCOMAT 2007, Herceg Novi, September 10-14, 2007. 2007;:180.
https://hdl.handle.net/21.15107/rcub_technorep_7369 .

Milojević, Mirjana, Najman, Stevo, Tomić, Simonida, Đorđević, Ljubiša, "Effect of hydrogels based on 2-hydroxyethyl methacrylates to erythrocyte resistance to hemolysis" in Programme and the Book of Abstracts / Ninth Annual Conference of the Yugoslav Materials Research Society YUCOMAT 2007, Herceg Novi, September 10-14, 2007 (2007):180,
https://hdl.handle.net/21.15107/rcub_technorep_7369 .

TY  - JOUR
AU  - Milojević, Mirjana
AU  - Najman, Stevo
AU  - Tomić, Simonida
PY  - 2007
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1187
AB  - The aim of this study was to examine the effect of hydrogels different chemical construction and synthesized under different conditions, to oxidative activity of rat peritoneal macrophages. The hydrogels are synthesized by radiation and chemical polymerization of 2-hydroxyethylmethacrylate (HEMA), copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with poly(alkylene glycol) (met)acrylates-Bisomers. After incubation materials with macrophages, the effect of hydrogels was examine. All of tested materials reduct chemiluminescent activity of zimozan stimulated macrophages. Radiation polimerized PHEMA shows high inhibitory effect than chemical polimerized PHEMA. Hydrogels with more itaconic acid show high reducity effect, too. Different conditions of polimerization show differences in reducity effects to chemiluminescent activity of macrophages only in definite (order) reference (relation) between components of copolymers.
AB  - U radu je ispitivan efekat hidrogelova različitog hemijskog sastava i sintetisanih pod različitim uslovima na oksidativnu aktivnost peritonelanih makrofaga pacova. Hidrogelovi su dobijeni termički (hemijski) i radijaciono indukovanom polimerizacijom 2-hidroksietil metakrilata (HEMA) kopolimerizacijom HEMA i itakonske kiseline (IK), kao i kopolimerizacijom HEMA, IK i poli(alkilen glikol) (met)akrilata-Bisomeri (BIS). Efekat hidrogelova je ispitivan posle inkubacije materijala sa makrofagima dobijenim peritonealnom lavažom. Svi ispitivani materijali redukuju hemiluminescentnu aktivnost makrofaga stimulisanih zimozanom. Radijaciono umreženi PHEMA pokazuje veći inhibitorni efekat od hemijski umreženog. Hidrogelovi sa više itakonske kiseline pokazuju veći redukujući efekat. Različit način umrežavanja pokazuje razlike u redukujućim efektima na hemiluminescentnu aktivnost makrofaga samo pri određenim molarnim odnosima komponenti kopolimera.
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika - Novi materijali
T1  - Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages
T1  - Efekat hidrogelova na bazi 2-hidroksietil metakrilata na hemiluminescentnu aktivnost peritonealnih makrofaga pacova
EP  - 8
IS  - 3
SP  - 5
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1187
ER  - 

@article{
author = "Milojević, Mirjana and Najman, Stevo and Tomić, Simonida",
year = "2007",
abstract = "The aim of this study was to examine the effect of hydrogels different chemical construction and synthesized under different conditions, to oxidative activity of rat peritoneal macrophages. The hydrogels are synthesized by radiation and chemical polymerization of 2-hydroxyethylmethacrylate (HEMA), copolymerization of HEMA with itaconic acid (IA), and copolymerization of HEMA, IA with poly(alkylene glycol) (met)acrylates-Bisomers. After incubation materials with macrophages, the effect of hydrogels was examine. All of tested materials reduct chemiluminescent activity of zimozan stimulated macrophages. Radiation polimerized PHEMA shows high inhibitory effect than chemical polimerized PHEMA. Hydrogels with more itaconic acid show high reducity effect, too. Different conditions of polimerization show differences in reducity effects to chemiluminescent activity of macrophages only in definite (order) reference (relation) between components of copolymers., U radu je ispitivan efekat hidrogelova različitog hemijskog sastava i sintetisanih pod različitim uslovima na oksidativnu aktivnost peritonelanih makrofaga pacova. Hidrogelovi su dobijeni termički (hemijski) i radijaciono indukovanom polimerizacijom 2-hidroksietil metakrilata (HEMA) kopolimerizacijom HEMA i itakonske kiseline (IK), kao i kopolimerizacijom HEMA, IK i poli(alkilen glikol) (met)akrilata-Bisomeri (BIS). Efekat hidrogelova je ispitivan posle inkubacije materijala sa makrofagima dobijenim peritonealnom lavažom. Svi ispitivani materijali redukuju hemiluminescentnu aktivnost makrofaga stimulisanih zimozanom. Radijaciono umreženi PHEMA pokazuje veći inhibitorni efekat od hemijski umreženog. Hidrogelovi sa više itakonske kiseline pokazuju veći redukujući efekat. Različit način umrežavanja pokazuje razlike u redukujućim efektima na hemiluminescentnu aktivnost makrofaga samo pri određenim molarnim odnosima komponenti kopolimera.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika - Novi materijali",
title = "Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages, Efekat hidrogelova na bazi 2-hidroksietil metakrilata na hemiluminescentnu aktivnost peritonealnih makrofaga pacova",
pages = "8-5",
number = "3",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1187"
}

Milojević, M., Najman, S.,& Tomić, S.. (2007). Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages. in Tehnika - Novi materijali
Savez inženjera i tehničara Srbije, Beograd., 16(3), 5-8.
https://hdl.handle.net/21.15107/rcub_technorep_1187

Milojević M, Najman S, Tomić S. Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages. in Tehnika - Novi materijali. 2007;16(3):5-8.
https://hdl.handle.net/21.15107/rcub_technorep_1187 .

Milojević, Mirjana, Najman, Stevo, Tomić, Simonida, "Effect of hydrogels based on 2-hydroxyethyl methacrilate to chemiluminescent activity of rat peritoneal macrophages" in Tehnika - Novi materijali, 16, no. 3 (2007):5-8,
https://hdl.handle.net/21.15107/rcub_technorep_1187 .

TY  - JOUR
AU  - Ignjatović, Nenad
AU  - Savić, Vojin
AU  - Najman, Stevo
AU  - Plavšić, Milenko
AU  - Uskoković, Dragan
PY  - 2001
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5350
AB  - Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C=O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found. Copyright © 2001 Elsevier Science Ltd.

Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C double bond O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found.
PB  - Elsevier Science Ltd
T2  - Biomaterials
T1  - A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy
EP  - 575
IS  - 6
SP  - 571
VL  - 22
DO  - 10.1016/S0142-9612(00)00215-5
ER  - 

@article{
author = "Ignjatović, Nenad and Savić, Vojin and Najman, Stevo and Plavšić, Milenko and Uskoković, Dragan",
year = "2001",
abstract = "Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C=O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found. Copyright © 2001 Elsevier Science Ltd.

Chemically synthesized hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial was studied in vivo. The biocomposite was implanted into Balb/c Singen mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After 1 week of testing in vivo the implanted sample gave a spectrum in which absorption bands arising from newly formed functional groups of amine and peptide can be seen. After 3 weeks, a spectrum with pronounced absorption bands at 3420 and 1650cm-1 assigned to newly generated collagen, a component of the extracellular connective-tissue matrix, was registered. Also, decrease of the intensity absorption band at 1760cm-1 originating from the C double bond O group of PLLA indicates bioresorption of the PLLA used. Analysis of the microstructure of the sample surface by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial used alone were found.",
publisher = "Elsevier Science Ltd",
journal = "Biomaterials",
title = "A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy",
pages = "575-571",
number = "6",
volume = "22",
doi = "10.1016/S0142-9612(00)00215-5"
}

Ignjatović, N., Savić, V., Najman, S., Plavšić, M.,& Uskoković, D.. (2001). A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy. in Biomaterials
Elsevier Science Ltd., 22(6), 571-575.
https://doi.org/10.1016/S0142-9612(00)00215-5

Ignjatović N, Savić V, Najman S, Plavšić M, Uskoković D. A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy. in Biomaterials. 2001;22(6):571-575.
doi:10.1016/S0142-9612(00)00215-5 .

Ignjatović, Nenad, Savić, Vojin, Najman, Stevo, Plavšić, Milenko, Uskoković, Dragan, "A study of HAp/PLLA composite as a substitute for bone powder, using FT-IR spectroscopy" in Biomaterials, 22, no. 6 (2001):571-575,
https://doi.org/10.1016/S0142-9612(00)00215-5 . .

TY  - CONF
AU  - Ignjatović, Nenad
AU  - Plavšić, Milenko
AU  - Najman, Stevo
AU  - Savić, Vojin
AU  - Uskoković, Dragan
PY  - 2000
UR  - https://dais.sanu.ac.rs/123456789/3859
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5313
AB  - Hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial with PLLA of 50,000 and 430,000 g/mole molecular weight was studied in vivo. The biocomposite with PLLA of both molecular weights was implanted into mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After one week of testing in vivo, the implanted samples gave spectra in which absorption bands arising from new-formed functional groups of amine and peptide can be seen. Analysis of the spectra revealed faster formation of peptide compounds when the biocomposite with PLLA of lower molecular weight was used. After 3 weeks, the spectra of the biocomposite of both compositions were registered with pronounced absorption bands at about 3420 and 1650 cm-1 assigned to new-generated collagen, a component of the extracellular connective-tissue matrix. Also, in the case of the biocomposite sample with PLLA of lower molecular weight, disappearance of the previously present absorption band at about 1760 cm-1 originating from the C double bond O group of PLLA indicates complete bioresorption of the PLLA used. Analysis of the microstructures of the sample surfaces by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase in the system with PLLA of lower molecular weight and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial alone were found.
PB  - Uetikon-Zuerich : Trans Tech Publ
C3  - Trends in Advanced Materials and Processes
T1  - Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy
EP  - 150
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_dais_3859
ER  - 

@conference{
author = "Ignjatović, Nenad and Plavšić, Milenko and Najman, Stevo and Savić, Vojin and Uskoković, Dragan",
year = "2000",
abstract = "Hydroxyapatite/poly-L-lactide (HAp/PLLA) composite biomaterial with PLLA of 50,000 and 430,000 g/mole molecular weight was studied in vivo. The biocomposite with PLLA of both molecular weights was implanted into mice and after 1 and 3 weeks removed from their organisms and analyzed by the FT-IR spectroscopy. After one week of testing in vivo, the implanted samples gave spectra in which absorption bands arising from new-formed functional groups of amine and peptide can be seen. Analysis of the spectra revealed faster formation of peptide compounds when the biocomposite with PLLA of lower molecular weight was used. After 3 weeks, the spectra of the biocomposite of both compositions were registered with pronounced absorption bands at about 3420 and 1650 cm-1 assigned to new-generated collagen, a component of the extracellular connective-tissue matrix. Also, in the case of the biocomposite sample with PLLA of lower molecular weight, disappearance of the previously present absorption band at about 1760 cm-1 originating from the C double bond O group of PLLA indicates complete bioresorption of the PLLA used. Analysis of the microstructures of the sample surfaces by scanning electron microscopy before and after implantation revealed bioresorption of the PLLA polymer phase in the system with PLLA of lower molecular weight and generation of collagen fibers at the sites of implanted bioresorptive PLLA. A mixture of autologous bone powder and HAp/PLLA biocomposite was also examined. After implantation, the same final products as in the case of HAp/PLLA composite biomaterial alone were found.",
publisher = "Uetikon-Zuerich : Trans Tech Publ",
journal = "Trends in Advanced Materials and Processes",
title = "Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy",
pages = "150-143",
url = "https://hdl.handle.net/21.15107/rcub_dais_3859"
}

Ignjatović, N., Plavšić, M., Najman, S., Savić, V.,& Uskoković, D.. (2000). Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy. in Trends in Advanced Materials and Processes
Uetikon-Zuerich : Trans Tech Publ., 143-150.
https://hdl.handle.net/21.15107/rcub_dais_3859

Ignjatović N, Plavšić M, Najman S, Savić V, Uskoković D. Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy. in Trends in Advanced Materials and Processes. 2000;:143-150.
https://hdl.handle.net/21.15107/rcub_dais_3859 .

Ignjatović, Nenad, Plavšić, Milenko, Najman, Stevo, Savić, Vojin, Uskoković, Dragan, "Analysis of in vivo substitution of bone tissue by HAp/PLLA composite biomaterial with PLLA of different molecular weights using FTIR spectroscopy" in Trends in Advanced Materials and Processes (2000):143-150,
https://hdl.handle.net/21.15107/rcub_dais_3859 .