TY  - JOUR
AU  - Bačić, Ana
AU  - Milivojević, Vladimir
AU  - Petković, Isidora
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Medić Brkić, Branislava
AU  - Popadić, Dušan
AU  - Milosavljević, Tomica
AU  - Rajilić-Stojanović, Mirjana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6054
AB  - Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.
T2  - Journal of Fungi
T1  - In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species
IS  - 3
SP  - 328
VL  - 9
DO  - 10.3390/jof9030328
ER  - 

@article{
author = "Bačić, Ana and Milivojević, Vladimir and Petković, Isidora and Kekić, Dušan and Gajić, Ina and Medić Brkić, Branislava and Popadić, Dušan and Milosavljević, Tomica and Rajilić-Stojanović, Mirjana",
year = "2023",
abstract = "Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.",
journal = "Journal of Fungi",
title = "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species",
number = "3",
pages = "328",
volume = "9",
doi = "10.3390/jof9030328"
}

Bačić, A., Milivojević, V., Petković, I., Kekić, D., Gajić, I., Medić Brkić, B., Popadić, D., Milosavljević, T.,& Rajilić-Stojanović, M.. (2023). In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi, 9(3), 328.
https://doi.org/10.3390/jof9030328

Bačić A, Milivojević V, Petković I, Kekić D, Gajić I, Medić Brkić B, Popadić D, Milosavljević T, Rajilić-Stojanović M. In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi. 2023;9(3):328.
doi:10.3390/jof9030328 .

Bačić, Ana, Milivojević, Vladimir, Petković, Isidora, Kekić, Dušan, Gajić, Ina, Medić Brkić, Branislava, Popadić, Dušan, Milosavljević, Tomica, Rajilić-Stojanović, Mirjana, "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species" in Journal of Fungi, 9, no. 3 (2023):328,
https://doi.org/10.3390/jof9030328 . .

TY  - JOUR
AU  - Cammarota, Giovanni
AU  - Ianiro, Gianluca
AU  - Tilg, Herbert
AU  - Rajilić-Stojanović, Mirjana
AU  - Kump, Patrizia
AU  - Satokari, Reetta
AU  - Sokol, Harry
AU  - Arkkila, Perttu
AU  - Pintus, Cristina
AU  - Hart, Ailsa
AU  - Segal, Jonathan
AU  - Aloi, Marina
AU  - Masucci, Luca
AU  - Molinaro, Antonio
AU  - Scaldaferri, Franco
AU  - Gasbarrini, Giovanni
AU  - Lopez-Sanroman, Antonio
AU  - Link, Alexander
AU  - De Groot, Pieter
AU  - de Vos, Willem M.
AU  - Hoegenauer, Christoph
AU  - Malfertheiner, Peter
AU  - Mattila, Eero
AU  - Milosavljević, Tomica
AU  - Nieuwdorp, Max
AU  - Sanguinetti, Maurizio
AU  - Simren, Magnus
AU  - Gasbarrini, Antonio
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3706
AB  - Faecal microbiota transplantation (FMT) is an important therapeutic option for Clostridium difficile infection. Promising findings suggest that FMT may play a role also in the management of other disorders associated with the alteration of gut microbiota. Although the health community is assessing FMT with renewed interest and patients are becoming more aware, there are technical and logistical issues in establishing such a non-standardised treatment into the clinical practice with safety and proper governance. In view of this, an evidence-based recommendation is needed to drive the practical implementation of FMT. In this European Consensus Conference, 28 experts from 10 countries collaborated, in separate working groups and through an evidence-based process, to provide statements on the following key issues: FMT indications; donor selection; preparation of faecal material; clinical management and faecal delivery and basic requirements for implementing an FMT centre. Statements developed by each working group were evaluated and voted by all members, first through an electronic Delphi process, and then in a plenary consensus conference. The recommendations were released according to best available evidence, in order to act as guidance for physicians who plan to implement FMT, aiming at supporting the broad availability of the procedure, discussing other issues relevant to FMT and promoting future clinical research in the area of gut microbiota manipulation. This consensus report strongly recommends the implementation of FMT centres for the treatment of C. difficile infection as well as traces the guidelines of technicality, regulatory, administrative and laboratory requirements.
PB  - Bmj Publishing Group, London
T2  - GUT
T1  - European consensus conference on faecal microbiota transplantation in clinical practice
EP  - 580
IS  - 4
SP  - 569
VL  - 66
DO  - 10.1136/gutjnl-2016-313017
ER  - 

@article{
author = "Cammarota, Giovanni and Ianiro, Gianluca and Tilg, Herbert and Rajilić-Stojanović, Mirjana and Kump, Patrizia and Satokari, Reetta and Sokol, Harry and Arkkila, Perttu and Pintus, Cristina and Hart, Ailsa and Segal, Jonathan and Aloi, Marina and Masucci, Luca and Molinaro, Antonio and Scaldaferri, Franco and Gasbarrini, Giovanni and Lopez-Sanroman, Antonio and Link, Alexander and De Groot, Pieter and de Vos, Willem M. and Hoegenauer, Christoph and Malfertheiner, Peter and Mattila, Eero and Milosavljević, Tomica and Nieuwdorp, Max and Sanguinetti, Maurizio and Simren, Magnus and Gasbarrini, Antonio",
year = "2017",
abstract = "Faecal microbiota transplantation (FMT) is an important therapeutic option for Clostridium difficile infection. Promising findings suggest that FMT may play a role also in the management of other disorders associated with the alteration of gut microbiota. Although the health community is assessing FMT with renewed interest and patients are becoming more aware, there are technical and logistical issues in establishing such a non-standardised treatment into the clinical practice with safety and proper governance. In view of this, an evidence-based recommendation is needed to drive the practical implementation of FMT. In this European Consensus Conference, 28 experts from 10 countries collaborated, in separate working groups and through an evidence-based process, to provide statements on the following key issues: FMT indications; donor selection; preparation of faecal material; clinical management and faecal delivery and basic requirements for implementing an FMT centre. Statements developed by each working group were evaluated and voted by all members, first through an electronic Delphi process, and then in a plenary consensus conference. The recommendations were released according to best available evidence, in order to act as guidance for physicians who plan to implement FMT, aiming at supporting the broad availability of the procedure, discussing other issues relevant to FMT and promoting future clinical research in the area of gut microbiota manipulation. This consensus report strongly recommends the implementation of FMT centres for the treatment of C. difficile infection as well as traces the guidelines of technicality, regulatory, administrative and laboratory requirements.",
publisher = "Bmj Publishing Group, London",
journal = "GUT",
title = "European consensus conference on faecal microbiota transplantation in clinical practice",
pages = "580-569",
number = "4",
volume = "66",
doi = "10.1136/gutjnl-2016-313017"
}

Cammarota, G., Ianiro, G., Tilg, H., Rajilić-Stojanović, M., Kump, P., Satokari, R., Sokol, H., Arkkila, P., Pintus, C., Hart, A., Segal, J., Aloi, M., Masucci, L., Molinaro, A., Scaldaferri, F., Gasbarrini, G., Lopez-Sanroman, A., Link, A., De Groot, P., de Vos, W. M., Hoegenauer, C., Malfertheiner, P., Mattila, E., Milosavljević, T., Nieuwdorp, M., Sanguinetti, M., Simren, M.,& Gasbarrini, A.. (2017). European consensus conference on faecal microbiota transplantation in clinical practice. in GUT
Bmj Publishing Group, London., 66(4), 569-580.
https://doi.org/10.1136/gutjnl-2016-313017

Cammarota G, Ianiro G, Tilg H, Rajilić-Stojanović M, Kump P, Satokari R, Sokol H, Arkkila P, Pintus C, Hart A, Segal J, Aloi M, Masucci L, Molinaro A, Scaldaferri F, Gasbarrini G, Lopez-Sanroman A, Link A, De Groot P, de Vos WM, Hoegenauer C, Malfertheiner P, Mattila E, Milosavljević T, Nieuwdorp M, Sanguinetti M, Simren M, Gasbarrini A. European consensus conference on faecal microbiota transplantation in clinical practice. in GUT. 2017;66(4):569-580.
doi:10.1136/gutjnl-2016-313017 .

Cammarota, Giovanni, Ianiro, Gianluca, Tilg, Herbert, Rajilić-Stojanović, Mirjana, Kump, Patrizia, Satokari, Reetta, Sokol, Harry, Arkkila, Perttu, Pintus, Cristina, Hart, Ailsa, Segal, Jonathan, Aloi, Marina, Masucci, Luca, Molinaro, Antonio, Scaldaferri, Franco, Gasbarrini, Giovanni, Lopez-Sanroman, Antonio, Link, Alexander, De Groot, Pieter, de Vos, Willem M., Hoegenauer, Christoph, Malfertheiner, Peter, Mattila, Eero, Milosavljević, Tomica, Nieuwdorp, Max, Sanguinetti, Maurizio, Simren, Magnus, Gasbarrini, Antonio, "European consensus conference on faecal microbiota transplantation in clinical practice" in GUT, 66, no. 4 (2017):569-580,
https://doi.org/10.1136/gutjnl-2016-313017 . .