TY  - JOUR
AU  - Bačić, Ana
AU  - Milivojević, Vladimir
AU  - Petković, Isidora
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Medić Brkić, Branislava
AU  - Popadić, Dušan
AU  - Milosavljević, Tomica
AU  - Rajilić-Stojanović, Mirjana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6054
AB  - Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.
T2  - Journal of Fungi
T1  - In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species
IS  - 3
SP  - 328
VL  - 9
DO  - 10.3390/jof9030328
ER  - 

@article{
author = "Bačić, Ana and Milivojević, Vladimir and Petković, Isidora and Kekić, Dušan and Gajić, Ina and Medić Brkić, Branislava and Popadić, Dušan and Milosavljević, Tomica and Rajilić-Stojanović, Mirjana",
year = "2023",
abstract = "Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.",
journal = "Journal of Fungi",
title = "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species",
number = "3",
pages = "328",
volume = "9",
doi = "10.3390/jof9030328"
}

Bačić, A., Milivojević, V., Petković, I., Kekić, D., Gajić, I., Medić Brkić, B., Popadić, D., Milosavljević, T.,& Rajilić-Stojanović, M.. (2023). In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi, 9(3), 328.
https://doi.org/10.3390/jof9030328

Bačić A, Milivojević V, Petković I, Kekić D, Gajić I, Medić Brkić B, Popadić D, Milosavljević T, Rajilić-Stojanović M. In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi. 2023;9(3):328.
doi:10.3390/jof9030328 .

Bačić, Ana, Milivojević, Vladimir, Petković, Isidora, Kekić, Dušan, Gajić, Ina, Medić Brkić, Branislava, Popadić, Dušan, Milosavljević, Tomica, Rajilić-Stojanović, Mirjana, "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species" in Journal of Fungi, 9, no. 3 (2023):328,
https://doi.org/10.3390/jof9030328 . .

TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Božić, Bojan
AU  - Lunić, Tanja
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Nedeljković Božić, Biljana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5097
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - MDPI
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis
IS  - 6
SP  - 1273
VL  - 14
DO  - 10.3390/nu14061273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Nedeljković Božić, Biljana",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12—VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "MDPI",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis",
number = "6",
pages = "1273",
volume = "14",
doi = "10.3390/nu14061273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Nedeljković Božić, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients
MDPI., 14(6), 1273.
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Nedeljković Božić B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients. 2022;14(6):1273.
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Nedeljković Božić, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis—Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis" in Nutrients, 14, no. 6 (2022):1273,
https://doi.org/10.3390/nu14061273 . .

TY  - CHAP
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6125
AB  - Over the course of human evolutionary path, our microbiota has co-evolved to achieve a solid relationship with the human host in various body sites. The most densely populated site is the distal gut and gut microbiota is responsible for many utterly important functions that differ throughout life to meet physiological needs at different ages. A trajectory of microbial development tightly follows that of the human host, with initial unstable and rapidly changing microbial communities that are gradually transformed into a stable microbial ecosystem. During the first years of life, environmental and intrinsic factors affect the microbial assemblage, which when unfavorable may induce microbial dysbiosis and consequently alter the immune system maturation and neurodevelopment. Although adulthood is characterized by long-term stability of the gut microbiota with greater resilience to stressors, profound detrimental factors may lead to alterations in microbial communities. Recently elucidated bidirectional connections between the gut microbiota and distal organs, highlight the association between dysbiosis and the onset of various non-communicable diseases. With emerging evidence showing the importance of diverse and rich microbial communities in maintaining homeostasis and health, gut microbiota appears as a novel highly potent therapeutic target to alleviate ongoing pathophysiological processes and further promote longevity. In this article the characteristics of gut microbiota across human lifespan are discussed, with additional reference to microbiota of ancient and rural societies, with an intention to gain a broader picture and to provide insight into the factors influencing microbial assemblage.
PB  - Elsevier
T2  - Comprehensive Gut Microbiota
T1  - Microbiota changes throughout life - an overview
EP  - 12
SP  - 1
VL  - 2
DO  - 10.1016/B978-0-12-819265-8.00107-8
ER  - 

@inbook{
author = "Bačić, Ana and Rajilić-Stojanović, Mirjana",
year = "2022",
abstract = "Over the course of human evolutionary path, our microbiota has co-evolved to achieve a solid relationship with the human host in various body sites. The most densely populated site is the distal gut and gut microbiota is responsible for many utterly important functions that differ throughout life to meet physiological needs at different ages. A trajectory of microbial development tightly follows that of the human host, with initial unstable and rapidly changing microbial communities that are gradually transformed into a stable microbial ecosystem. During the first years of life, environmental and intrinsic factors affect the microbial assemblage, which when unfavorable may induce microbial dysbiosis and consequently alter the immune system maturation and neurodevelopment. Although adulthood is characterized by long-term stability of the gut microbiota with greater resilience to stressors, profound detrimental factors may lead to alterations in microbial communities. Recently elucidated bidirectional connections between the gut microbiota and distal organs, highlight the association between dysbiosis and the onset of various non-communicable diseases. With emerging evidence showing the importance of diverse and rich microbial communities in maintaining homeostasis and health, gut microbiota appears as a novel highly potent therapeutic target to alleviate ongoing pathophysiological processes and further promote longevity. In this article the characteristics of gut microbiota across human lifespan are discussed, with additional reference to microbiota of ancient and rural societies, with an intention to gain a broader picture and to provide insight into the factors influencing microbial assemblage.",
publisher = "Elsevier",
journal = "Comprehensive Gut Microbiota",
booktitle = "Microbiota changes throughout life - an overview",
pages = "12-1",
volume = "2",
doi = "10.1016/B978-0-12-819265-8.00107-8"
}

Bačić, A.,& Rajilić-Stojanović, M.. (2022). Microbiota changes throughout life - an overview. in Comprehensive Gut Microbiota
Elsevier., 2, 1-12.
https://doi.org/10.1016/B978-0-12-819265-8.00107-8

Bačić A, Rajilić-Stojanović M. Microbiota changes throughout life - an overview. in Comprehensive Gut Microbiota. 2022;2:1-12.
doi:10.1016/B978-0-12-819265-8.00107-8 .

Bačić, Ana, Rajilić-Stojanović, Mirjana, "Microbiota changes throughout life - an overview" in Comprehensive Gut Microbiota, 2 (2022):1-12,
https://doi.org/10.1016/B978-0-12-819265-8.00107-8 . .