TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Carević, Milica
AU  - Bezbradica, Dejan
AU  - Bulatović, Maja
AU  - Rakin, Marica
AU  - Krunić, Tanja
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6123
AB  - Galacto-oligosaccharides (GOS) are produced from milk lactose during transgalactosylation reaction using  -galactosidase. Depending on reaction condition,  -galactosidase can, also, hydrolyse lactose, in order to make beverage more suitable for lactose intolerant individuals. GOAL: The aim of this study was to evaluate the prebiotic effect of the GOS, inulin and lactulose on growth and viability of probiotic bacteria in fermented whey based beverage. METHOD: Mixture of cow’s milk and whey (30%:70%) was subjected to transgalactosylation reaction using commercial β-galactosidase isolated from Aspergillus oryzae (0,5 mg/l). Before fermentation, samples were heat treated 15 minutes at 90 0C and after cooling on fermentation temperature (42 0C), samples were inoculated with lyophilized ABY-6 starter cultures. After fermentation and during refrigerated storage (28 days), samples were analyzed by measuring: pH value, titratable acidity, carbohydrate concentrations, total number of probiotic bacteria cells and syneresis. RESULT: Higher concentration of galacto-oligosaccharides was reached after 1,5h since, in the further course of transgalactosylation reaction, some amount of galacto-oligosaccharides were hydrolyzed to glucose and galactose. Slightly shorter fermentation time and higher viable cell number of probiotic bacteria were noticed in fermented whey based beverages with galacto-oligosaccharides than in beverages with inulin, lactulose and without prebiotics. During 28 days storage, changes in pH value and titratable acidity were not different significantly among beverages. Beverages with galacto-oligosaccharides had less syneresis and curd showed a better water holding capacity than other tested samples. After 28 days of storage, presence of different prebiotics in samples did not significantly influenced total number of probiotic bacteria cells (Lactobacillus acidophilus and Bifidobacterium bifidum), but in samples with galacto-oligosaccharides were noticed slightly higher content of Bifidobacterium bifidum. CONCLUSION: Regular use of fermented dairy beverages with galacto-oligosaccharides and probiotic bacteria, could favorably affect human health, modulating the composition of the colonic microflora.
PB  - UDRUŽENJE MIKROBIOLOGA SRBIJE
C3  - XI Kongres mikrobiologa Srbije - MIKROMED 2017
T1  - The effect of galacto-oligosaccharides on growth and viability of probiotic bacteria in fermented whey based bevereage
EP  - 199
SP  - 198
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6123
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Carević, Milica and Bezbradica, Dejan and Bulatović, Maja and Rakin, Marica and Krunić, Tanja",
year = "2017",
abstract = "Galacto-oligosaccharides (GOS) are produced from milk lactose during transgalactosylation reaction using  -galactosidase. Depending on reaction condition,  -galactosidase can, also, hydrolyse lactose, in order to make beverage more suitable for lactose intolerant individuals. GOAL: The aim of this study was to evaluate the prebiotic effect of the GOS, inulin and lactulose on growth and viability of probiotic bacteria in fermented whey based beverage. METHOD: Mixture of cow’s milk and whey (30%:70%) was subjected to transgalactosylation reaction using commercial β-galactosidase isolated from Aspergillus oryzae (0,5 mg/l). Before fermentation, samples were heat treated 15 minutes at 90 0C and after cooling on fermentation temperature (42 0C), samples were inoculated with lyophilized ABY-6 starter cultures. After fermentation and during refrigerated storage (28 days), samples were analyzed by measuring: pH value, titratable acidity, carbohydrate concentrations, total number of probiotic bacteria cells and syneresis. RESULT: Higher concentration of galacto-oligosaccharides was reached after 1,5h since, in the further course of transgalactosylation reaction, some amount of galacto-oligosaccharides were hydrolyzed to glucose and galactose. Slightly shorter fermentation time and higher viable cell number of probiotic bacteria were noticed in fermented whey based beverages with galacto-oligosaccharides than in beverages with inulin, lactulose and without prebiotics. During 28 days storage, changes in pH value and titratable acidity were not different significantly among beverages. Beverages with galacto-oligosaccharides had less syneresis and curd showed a better water holding capacity than other tested samples. After 28 days of storage, presence of different prebiotics in samples did not significantly influenced total number of probiotic bacteria cells (Lactobacillus acidophilus and Bifidobacterium bifidum), but in samples with galacto-oligosaccharides were noticed slightly higher content of Bifidobacterium bifidum. CONCLUSION: Regular use of fermented dairy beverages with galacto-oligosaccharides and probiotic bacteria, could favorably affect human health, modulating the composition of the colonic microflora.",
publisher = "UDRUŽENJE MIKROBIOLOGA SRBIJE",
journal = "XI Kongres mikrobiologa Srbije - MIKROMED 2017",
title = "The effect of galacto-oligosaccharides on growth and viability of probiotic bacteria in fermented whey based bevereage",
pages = "199-198",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6123"
}

Vukašinović-Sekulić, M., Carević, M., Bezbradica, D., Bulatović, M., Rakin, M.,& Krunić, T.. (2017). The effect of galacto-oligosaccharides on growth and viability of probiotic bacteria in fermented whey based bevereage. in XI Kongres mikrobiologa Srbije - MIKROMED 2017
UDRUŽENJE MIKROBIOLOGA SRBIJE., 198-199.
https://hdl.handle.net/21.15107/rcub_technorep_6123

Vukašinović-Sekulić M, Carević M, Bezbradica D, Bulatović M, Rakin M, Krunić T. The effect of galacto-oligosaccharides on growth and viability of probiotic bacteria in fermented whey based bevereage. in XI Kongres mikrobiologa Srbije - MIKROMED 2017. 2017;:198-199.
https://hdl.handle.net/21.15107/rcub_technorep_6123 .

Vukašinović-Sekulić, Maja, Carević, Milica, Bezbradica, Dejan, Bulatović, Maja, Rakin, Marica, Krunić, Tanja, "The effect of galacto-oligosaccharides on growth and viability of probiotic bacteria in fermented whey based bevereage" in XI Kongres mikrobiologa Srbije - MIKROMED 2017 (2017):198-199,
https://hdl.handle.net/21.15107/rcub_technorep_6123 .

TY  - CONF
AU  - Ćorović, Marija
AU  - Banjanac, Katarina
AU  - Prlainović, Nevena
AU  - Milisavljević, Ana
AU  - Carević, Milica
AU  - Marinković, Aleksandar
AU  - Bezbradica, Dejan
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6074
AB  - Lipases immobilized onto nonporous fumed silica nanoparticles (FNS) proved to be suitable catalysts for the various commercially important esters synthesis. This inert hydrophilic material is usually modified by different organosilanes, in order to assure formation of strong covalent bonds and/or hydrophobic interactions with enzyme surface amino acid residues. Hereby, we present methods for the modification of FNS surface by 3-aminopropyltrimethoxysilane (APTMS) and/or (Nphenylamino) propyltrimethoxysilane (PAPMS), and subsequent functionalization of amino-modified FNS nanoparticles with cyanuric-chloride (CC). Selected modified nanocarriers were applied for the immobilization of Candida antarctica lipase B (CALB). Influence of chemical modification of FNS surface on the CALB immobilization process, protein loading, and hydrolytic activity of obtained preparations were examined. Furthermore, chosen derivatives were tested in the production of valuable food additive, liposoluble antioxidant, L-ascorbyl oleate.
Results showed that highest protein loadings were accomplished with nanocarriers modified with
combination of both APTMS and PAPMS. However, probably due to hindered approach of substrate molecules to the enzyme active sites, their hydrolytic and esterification activities were not
proportionally high. All preparations obtained with modified FNS nanoparticles were more active in the hydrolysis of p-nitrophenyl butyrate (p-NPB) comparing to CALB immobilized onto unmodified FNS. The highest hydrolytic activity of 1518.6 p-NPB/g was demonstrated by CALB covalently immobilized onto nanoparticles modified by APTMS and CC. Meanwhile, best results in the synthesis of L-ascorbyl oleate were achieved when CALB hydrophobically immobilized onto FNS nanoparticles modified by only PAPMS, with hydrolytic activity of 1200.2 p-NPB/g, was used as a catalyst and yield of 23.4 mM was accomplished.
Therefore, it was proven that different modification strategies ensured formation of different
interactions between lipase molecules and silica nanoparticles during immobilization, which led to
diverse hydrolytic and esterifying characteristics of obtained preparations. CALB immobilized onto
nanocarrier of highest hydrophobicity showed greatest potential for the production of L-ascorbyl
oleate.
PB  - University of Novi Sad, Institute of Food Technology
C3  - III International congress “Food technology, quality and safety”, Novi Sad
T1  - Immobilization of Candida antarctica lipase B onto modified silica nanoparticles and its application for the synthesis of l-ascorbyl oleate
EP  - 192
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6074
ER  - 

@conference{
author = "Ćorović, Marija and Banjanac, Katarina and Prlainović, Nevena and Milisavljević, Ana and Carević, Milica and Marinković, Aleksandar and Bezbradica, Dejan",
year = "2016",
abstract = "Lipases immobilized onto nonporous fumed silica nanoparticles (FNS) proved to be suitable catalysts for the various commercially important esters synthesis. This inert hydrophilic material is usually modified by different organosilanes, in order to assure formation of strong covalent bonds and/or hydrophobic interactions with enzyme surface amino acid residues. Hereby, we present methods for the modification of FNS surface by 3-aminopropyltrimethoxysilane (APTMS) and/or (Nphenylamino) propyltrimethoxysilane (PAPMS), and subsequent functionalization of amino-modified FNS nanoparticles with cyanuric-chloride (CC). Selected modified nanocarriers were applied for the immobilization of Candida antarctica lipase B (CALB). Influence of chemical modification of FNS surface on the CALB immobilization process, protein loading, and hydrolytic activity of obtained preparations were examined. Furthermore, chosen derivatives were tested in the production of valuable food additive, liposoluble antioxidant, L-ascorbyl oleate.
Results showed that highest protein loadings were accomplished with nanocarriers modified with
combination of both APTMS and PAPMS. However, probably due to hindered approach of substrate molecules to the enzyme active sites, their hydrolytic and esterification activities were not
proportionally high. All preparations obtained with modified FNS nanoparticles were more active in the hydrolysis of p-nitrophenyl butyrate (p-NPB) comparing to CALB immobilized onto unmodified FNS. The highest hydrolytic activity of 1518.6 p-NPB/g was demonstrated by CALB covalently immobilized onto nanoparticles modified by APTMS and CC. Meanwhile, best results in the synthesis of L-ascorbyl oleate were achieved when CALB hydrophobically immobilized onto FNS nanoparticles modified by only PAPMS, with hydrolytic activity of 1200.2 p-NPB/g, was used as a catalyst and yield of 23.4 mM was accomplished.
Therefore, it was proven that different modification strategies ensured formation of different
interactions between lipase molecules and silica nanoparticles during immobilization, which led to
diverse hydrolytic and esterifying characteristics of obtained preparations. CALB immobilized onto
nanocarrier of highest hydrophobicity showed greatest potential for the production of L-ascorbyl
oleate.",
publisher = "University of Novi Sad, Institute of Food Technology",
journal = "III International congress “Food technology, quality and safety”, Novi Sad",
title = "Immobilization of Candida antarctica lipase B onto modified silica nanoparticles and its application for the synthesis of l-ascorbyl oleate",
pages = "192-186",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6074"
}

Ćorović, M., Banjanac, K., Prlainović, N., Milisavljević, A., Carević, M., Marinković, A.,& Bezbradica, D.. (2016). Immobilization of Candida antarctica lipase B onto modified silica nanoparticles and its application for the synthesis of l-ascorbyl oleate. in III International congress “Food technology, quality and safety”, Novi Sad
University of Novi Sad, Institute of Food Technology., 186-192.
https://hdl.handle.net/21.15107/rcub_technorep_6074

Ćorović M, Banjanac K, Prlainović N, Milisavljević A, Carević M, Marinković A, Bezbradica D. Immobilization of Candida antarctica lipase B onto modified silica nanoparticles and its application for the synthesis of l-ascorbyl oleate. in III International congress “Food technology, quality and safety”, Novi Sad. 2016;:186-192.
https://hdl.handle.net/21.15107/rcub_technorep_6074 .

Ćorović, Marija, Banjanac, Katarina, Prlainović, Nevena, Milisavljević, Ana, Carević, Milica, Marinković, Aleksandar, Bezbradica, Dejan, "Immobilization of Candida antarctica lipase B onto modified silica nanoparticles and its application for the synthesis of l-ascorbyl oleate" in III International congress “Food technology, quality and safety”, Novi Sad (2016):186-192,
https://hdl.handle.net/21.15107/rcub_technorep_6074 .

TY  - THES
AU  - Carević, Milica
PY  - 2016
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6075
AB  - U okviru ove doktorske disertacije ispitan je veliki broj mikroorganizama iz
kolekcije kultura Tehnološko-metalurškog fakulteta Univerziteta u Beogradu u
cilju izbora najefikasnijeg producenta β-galaktozidaze. Kao najbolji producent sa
aspekta aktivnosti, ali i karakteristika dobijenog enzima izdvojila se probiotska
vrsta Lactobacillus acidophilus. Stoga, u cilju poboljšanja procesa produkcije β-
galaktozidaze, u nastavku rada izvršena je detaljna optimizacija sastava hranljivih
podloga, parametara procesa proizvodnje, kao i izolovanja enzima. Okarakterisana
su najbitnija svojstva izolovane β-galaktozidaze iz L. acidophilus (tremperaturna i
pH aktivnost/stabilnost, kinetički parametri) na osnovu čega je potom upoređena
sa komercijalnim preparatom β-galaktozidaze iz A. oryzae. Oba enzima primenjena
su u reakciji transgalaktozilacije za sintezu galakto-oligosaharida. )majući u vidu
kompleksnost ove reakcije, identifikovani su i ispitani uticaji ključnih reakcionih
parametara: koncentracije enzima i laktoze, kao i vremena trajanja reakcije. Pored
optimizacije prinosa, određena je i detaljna struktura dobijenih galaktooligosaharida.
U tu svrhu razvijena je brza i efikasna jednostepena metoda
primenom spektrometrije jonske pokretljivosti spregnute sa masenom
spektroskopijom (IMS/MS). Ustanovljeno je da galakto-oligosaharidi dobijeni u
reakcijama katalizovanim pomoću β-galaktozidaze iz A. oryzae uglavnom sadrže
β(1→6) i β (1→3) glikozidne veze, dok galakto-oligosaharidi dobijeni pomoću β-
galaktozidaze iz L. acidophilus sadrže β(1→6) i β(1→4) veze. Takođe, utvrđeno je
da su dobijeni proizvodi različitih stepena polimerizacije, ali i što je najbitnije da se
udeo pojedinih veza menja sa vremenom reakcije. U cilju poboljšanja stabilnosti
ispitivanih enzimskih preparata, i njihove implementacije u kontinualnim
reaktorskim sistemima, izvršena je imobilizacija enzima na čvrste nosače. )spitano
je više modifikovanih i nemodifikovanih nosača za imobilizaciju različitih
karakteristika posredstvom različitih mehanizama vezivanja, pri čemu je pokazano
da su za imobilizaciju oba enzima najpogodniji nosači sa primarnim amino
grupama, koje omogućavaju adsorbciju enzima elektrostatičkim interakcijama.
Najbolji rezultati u slučaju komercijalnog enezima iz A. oryzae postignuti su sa
komercijalnim nosačem Purolite® A109, pri čemu je postignuto znatno
unapređenje procesa hemijskom aktivacijom karboksilnih grupa na površini
enzima, što je dovelo do formiranja kovalentnih veza između nosača i enzima.
Ovom imobilizacijom postignuto je značajno povećanje termičke i operativne
stabilnosti, ali i povećanje specifičnosti prema reakciji transgalaktozilacije. Sa
druge strane, izolovani enzim iz L. acidophilus uspešno je imobilisan na
komercijalni nosač Lifetech ECR8409. Imobilisani preparati β-galaktozidaza potom
primenjeni su u šaržnom i bioreaktoru sa fluidizovanim slojem enzima i
recirkulacijom supstrata. U oba reaktora izvršena je optimizacija relevantnih
procesnih parametara, a rezultati su pokazali da se reakcije u oba enzima odvijaju
približno jednakom brzinom, ali je stabilnost enzima i reaktoru sa fluidizovanim
slojem povećana, pa se imobilisani enzim može koristiti u većem broju reakcionih
ciklusa. U poslednjem delu ove doktorske disertacije, ispitana je mogućnost
korišćenja alternativnih akceptora galaktozil ostataka, salicina i sorbitola, u
reakciji transgalaktozilacije, pri čemu kao proizvod reakcije nastaju nova fiziološki
aktivna jedinjenja. Oba enzima pokazala su visok afinitet prema sintezi novih
proizvoda, a osnovni parametri reakcije optimizovani su primenom metode
statističkog planiranja eksperimenata, pri čemu su ustanovljeni najznačajniji uticaji
ključnih parametara, ali i njihove međusobne interakcije. U oba slučaja utvrđeno je
da su najbolji prinosi postignuti pri visokim koncentracijama laktoze, kao i u
prisustvu akceptora (salicina ili sorbitola) u višku.
PB  - [Carević, M. V.]
T1  - Proizvodnja i imobilizacija mikrobnih β-galaktozidaza za primenu u transgalaktozilacionim reakcijama
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6075
ER  - 

@phdthesis{
author = "Carević, Milica",
year = "2016",
abstract = "U okviru ove doktorske disertacije ispitan je veliki broj mikroorganizama iz
kolekcije kultura Tehnološko-metalurškog fakulteta Univerziteta u Beogradu u
cilju izbora najefikasnijeg producenta β-galaktozidaze. Kao najbolji producent sa
aspekta aktivnosti, ali i karakteristika dobijenog enzima izdvojila se probiotska
vrsta Lactobacillus acidophilus. Stoga, u cilju poboljšanja procesa produkcije β-
galaktozidaze, u nastavku rada izvršena je detaljna optimizacija sastava hranljivih
podloga, parametara procesa proizvodnje, kao i izolovanja enzima. Okarakterisana
su najbitnija svojstva izolovane β-galaktozidaze iz L. acidophilus (tremperaturna i
pH aktivnost/stabilnost, kinetički parametri) na osnovu čega je potom upoređena
sa komercijalnim preparatom β-galaktozidaze iz A. oryzae. Oba enzima primenjena
su u reakciji transgalaktozilacije za sintezu galakto-oligosaharida. )majući u vidu
kompleksnost ove reakcije, identifikovani su i ispitani uticaji ključnih reakcionih
parametara: koncentracije enzima i laktoze, kao i vremena trajanja reakcije. Pored
optimizacije prinosa, određena je i detaljna struktura dobijenih galaktooligosaharida.
U tu svrhu razvijena je brza i efikasna jednostepena metoda
primenom spektrometrije jonske pokretljivosti spregnute sa masenom
spektroskopijom (IMS/MS). Ustanovljeno je da galakto-oligosaharidi dobijeni u
reakcijama katalizovanim pomoću β-galaktozidaze iz A. oryzae uglavnom sadrže
β(1→6) i β (1→3) glikozidne veze, dok galakto-oligosaharidi dobijeni pomoću β-
galaktozidaze iz L. acidophilus sadrže β(1→6) i β(1→4) veze. Takođe, utvrđeno je
da su dobijeni proizvodi različitih stepena polimerizacije, ali i što je najbitnije da se
udeo pojedinih veza menja sa vremenom reakcije. U cilju poboljšanja stabilnosti
ispitivanih enzimskih preparata, i njihove implementacije u kontinualnim
reaktorskim sistemima, izvršena je imobilizacija enzima na čvrste nosače. )spitano
je više modifikovanih i nemodifikovanih nosača za imobilizaciju različitih
karakteristika posredstvom različitih mehanizama vezivanja, pri čemu je pokazano
da su za imobilizaciju oba enzima najpogodniji nosači sa primarnim amino
grupama, koje omogućavaju adsorbciju enzima elektrostatičkim interakcijama.
Najbolji rezultati u slučaju komercijalnog enezima iz A. oryzae postignuti su sa
komercijalnim nosačem Purolite® A109, pri čemu je postignuto znatno
unapređenje procesa hemijskom aktivacijom karboksilnih grupa na površini
enzima, što je dovelo do formiranja kovalentnih veza između nosača i enzima.
Ovom imobilizacijom postignuto je značajno povećanje termičke i operativne
stabilnosti, ali i povećanje specifičnosti prema reakciji transgalaktozilacije. Sa
druge strane, izolovani enzim iz L. acidophilus uspešno je imobilisan na
komercijalni nosač Lifetech ECR8409. Imobilisani preparati β-galaktozidaza potom
primenjeni su u šaržnom i bioreaktoru sa fluidizovanim slojem enzima i
recirkulacijom supstrata. U oba reaktora izvršena je optimizacija relevantnih
procesnih parametara, a rezultati su pokazali da se reakcije u oba enzima odvijaju
približno jednakom brzinom, ali je stabilnost enzima i reaktoru sa fluidizovanim
slojem povećana, pa se imobilisani enzim može koristiti u većem broju reakcionih
ciklusa. U poslednjem delu ove doktorske disertacije, ispitana je mogućnost
korišćenja alternativnih akceptora galaktozil ostataka, salicina i sorbitola, u
reakciji transgalaktozilacije, pri čemu kao proizvod reakcije nastaju nova fiziološki
aktivna jedinjenja. Oba enzima pokazala su visok afinitet prema sintezi novih
proizvoda, a osnovni parametri reakcije optimizovani su primenom metode
statističkog planiranja eksperimenata, pri čemu su ustanovljeni najznačajniji uticaji
ključnih parametara, ali i njihove međusobne interakcije. U oba slučaja utvrđeno je
da su najbolji prinosi postignuti pri visokim koncentracijama laktoze, kao i u
prisustvu akceptora (salicina ili sorbitola) u višku.",
publisher = "[Carević, M. V.]",
title = "Proizvodnja i imobilizacija mikrobnih β-galaktozidaza za primenu u transgalaktozilacionim reakcijama",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6075"
}

Carević, M.. (2016). Proizvodnja i imobilizacija mikrobnih β-galaktozidaza za primenu u transgalaktozilacionim reakcijama. 
[Carević, M. V.]..
https://hdl.handle.net/21.15107/rcub_technorep_6075

Carević M. Proizvodnja i imobilizacija mikrobnih β-galaktozidaza za primenu u transgalaktozilacionim reakcijama. 2016;.
https://hdl.handle.net/21.15107/rcub_technorep_6075 .

Carević, Milica, "Proizvodnja i imobilizacija mikrobnih β-galaktozidaza za primenu u transgalaktozilacionim reakcijama" (2016),
https://hdl.handle.net/21.15107/rcub_technorep_6075 .

TY  - CONF
AU  - Jakovetić, Sonja
AU  - Luković, Nevena
AU  - Grbavčić, Sanja
AU  - Jovanović, Jelena
AU  - Stefanović, Andrea
AU  - Carević, Milica
AU  - Knežević-Jugović, Zorica
PY  - 2013
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6212
AB  - Increased interest in phenolic acids stems from their physiological benefits, which make them very interesting for various applications in food and pharmaceutical industries [1]. The main advantage of phenolic compounds application as natural antioxidants instead of traditionally used synthetic ones, butylated hydroxytoluen (BHT) and butylated hydroxyanisole (BHA), is found in the fact that both BHT and BHA are potential carcinogens, hence their application continuously decreases [2]. On the other hand, one of the main obstacles for their utilization in foods and cosmetics is their low solubility in emulsions and oil-based formulas as repercussion of their hydrophilic nature. Introduction of aliphatic side chain groups via enzyme-catalyzed esterification, causing solubility alternations of phenolic acids, is an adequate way for increasing their industrial application potential [3]. The aim of this research was to enable efficient enzyme-catalyzed synthesis of liposoluble phenolic esters using synthesis oleyl cinnamate as model reaction and to determine kinetic constants which are very important for process scale up. Enzyme used for all esterifications is commercial preparation of lipase B from Candida antarctica, Novozyme 435. Initial kinetic study was performed in capped vessels where different concentrations of cinnamic acid (0.1-0.7 mol dm-3), oleyl alcohol (0.1-0.7 mol dm-3) and immobilized lipase (40 mg) were mixed in isooctane and put on orbital shaker set at 55°C and 150 rpm for 6 hours. Several different models were used to fit experimentally obtained data, but the best fit was obtained when ping-pong bi-bi model was used.
PB  - Serbian Chemical Society
C3  - ICOSECS8
T1  - The kinetic study of oleylcinnamate synthesis
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6212
ER  - 

@conference{
author = "Jakovetić, Sonja and Luković, Nevena and Grbavčić, Sanja and Jovanović, Jelena and Stefanović, Andrea and Carević, Milica and Knežević-Jugović, Zorica",
year = "2013",
abstract = "Increased interest in phenolic acids stems from their physiological benefits, which make them very interesting for various applications in food and pharmaceutical industries [1]. The main advantage of phenolic compounds application as natural antioxidants instead of traditionally used synthetic ones, butylated hydroxytoluen (BHT) and butylated hydroxyanisole (BHA), is found in the fact that both BHT and BHA are potential carcinogens, hence their application continuously decreases [2]. On the other hand, one of the main obstacles for their utilization in foods and cosmetics is their low solubility in emulsions and oil-based formulas as repercussion of their hydrophilic nature. Introduction of aliphatic side chain groups via enzyme-catalyzed esterification, causing solubility alternations of phenolic acids, is an adequate way for increasing their industrial application potential [3]. The aim of this research was to enable efficient enzyme-catalyzed synthesis of liposoluble phenolic esters using synthesis oleyl cinnamate as model reaction and to determine kinetic constants which are very important for process scale up. Enzyme used for all esterifications is commercial preparation of lipase B from Candida antarctica, Novozyme 435. Initial kinetic study was performed in capped vessels where different concentrations of cinnamic acid (0.1-0.7 mol dm-3), oleyl alcohol (0.1-0.7 mol dm-3) and immobilized lipase (40 mg) were mixed in isooctane and put on orbital shaker set at 55°C and 150 rpm for 6 hours. Several different models were used to fit experimentally obtained data, but the best fit was obtained when ping-pong bi-bi model was used.",
publisher = "Serbian Chemical Society",
journal = "ICOSECS8",
title = "The kinetic study of oleylcinnamate synthesis",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6212"
}

Jakovetić, S., Luković, N., Grbavčić, S., Jovanović, J., Stefanović, A., Carević, M.,& Knežević-Jugović, Z.. (2013). The kinetic study of oleylcinnamate synthesis. in ICOSECS8
Serbian Chemical Society..
https://hdl.handle.net/21.15107/rcub_technorep_6212

Jakovetić S, Luković N, Grbavčić S, Jovanović J, Stefanović A, Carević M, Knežević-Jugović Z. The kinetic study of oleylcinnamate synthesis. in ICOSECS8. 2013;.
https://hdl.handle.net/21.15107/rcub_technorep_6212 .

Jakovetić, Sonja, Luković, Nevena, Grbavčić, Sanja, Jovanović, Jelena, Stefanović, Andrea, Carević, Milica, Knežević-Jugović, Zorica, "The kinetic study of oleylcinnamate synthesis" in ICOSECS8 (2013),
https://hdl.handle.net/21.15107/rcub_technorep_6212 .