TY  - JOUR
AU  - Veljković, Milica
AU  - Stepanović, Relja
AU  - Banjanac, Katarina
AU  - Ćorović, Marija
AU  - Milivojević, Ana
AU  - Simović, Milica
AU  - Milivojević, Milan
AU  - Bezbradica, Dejan
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5247
AB  - Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.
PB  - Korean Society of Industrial Engineering Chemistry
T2  - Journal of Industrial and Engineering Chemistry
T1  - Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109
EP  - 156
SP  - 149
VL  - 117
DO  - 10.1016/j.jiec.2022.09.051
ER  - 

@article{
author = "Veljković, Milica and Stepanović, Relja and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Milivojević, Milan and Bezbradica, Dejan",
year = "2023",
abstract = "Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.",
publisher = "Korean Society of Industrial Engineering Chemistry",
journal = "Journal of Industrial and Engineering Chemistry",
title = "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109",
pages = "156-149",
volume = "117",
doi = "10.1016/j.jiec.2022.09.051"
}

Veljković, M., Stepanović, R., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Milivojević, M.,& Bezbradica, D.. (2023). Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry
Korean Society of Industrial Engineering Chemistry., 117, 149-156.
https://doi.org/10.1016/j.jiec.2022.09.051

Veljković M, Stepanović R, Banjanac K, Ćorović M, Milivojević A, Simović M, Milivojević M, Bezbradica D. Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry. 2023;117:149-156.
doi:10.1016/j.jiec.2022.09.051 .

Veljković, Milica, Stepanović, Relja, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Milivojević, Milan, Bezbradica, Dejan, "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109" in Journal of Industrial and Engineering Chemistry, 117 (2023):149-156,
https://doi.org/10.1016/j.jiec.2022.09.051 . .