Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109
Само за регистроване кориснике
2023
Аутори
Veljković, MilicaStepanović, Relja
Banjanac, Katarina
Ćorović, Marija
Milivojević, Ana
Simović, Milica
Milivojević, Milan
Bezbradica, Dejan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exception...ally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.
Кључне речи:
Air-lift reactor / Fructo-oligosaccharides / Fructosyltransferase / Purolite® A109 / Selective immobilizationИзвор:
Journal of Industrial and Engineering Chemistry, 2023, 117, 149-156Издавач:
- Korean Society of Industrial Engineering Chemistry
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Science Fund of the Republic of Serbia (Programe IDEAS, PrIntPrEnzy, project ID 7750109).
Колекције
Институција/група
Inovacioni centarTY - JOUR AU - Veljković, Milica AU - Stepanović, Relja AU - Banjanac, Katarina AU - Ćorović, Marija AU - Milivojević, Ana AU - Simović, Milica AU - Milivojević, Milan AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5247 AB - Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems. PB - Korean Society of Industrial Engineering Chemistry T2 - Journal of Industrial and Engineering Chemistry T1 - Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109 EP - 156 SP - 149 VL - 117 DO - 10.1016/j.jiec.2022.09.051 ER -
@article{ author = "Veljković, Milica and Stepanović, Relja and Banjanac, Katarina and Ćorović, Marija and Milivojević, Ana and Simović, Milica and Milivojević, Milan and Bezbradica, Dejan", year = "2023", abstract = "Within this study, the investigation of potential selective immobilization of fructosyltransferase (FTase) derived from Aspergillus aculeatus (from commercial enzyme preparation Pectinex® Ultra SP-L) onto the polystyrenic macroporous resin Purolite® A109 and its further utilization for the synthesis of a highly valuable group of prebiotic compounds named fructo-oligosaccharides (FOS), was performed. The obtained results suggest that partial purification of fructosyltransferase from complex enzyme mixture with predominant pectinase activity (ratio of activity immobilization yields around 20.0) occurred by performing a simple immobilization protocol, provided that immobilization lasted for 5 h using an enzyme concentration of 50 mg g−1 of support under slightly acidic conditions (pH 6.0). Immobilized preparation was thereafter successfully utilized for FOS synthesis (56–59 % FOS of total sugars) in both a batch and air-lift reactor system. Operational stability study proved the exceptionally high potential of immobilized preparation for reproducible and cost-effective application, since 90 % of initial activity was preserved after ten consecutive reaction cycles. Thereafter, a prospectus for continuous production of FOS in the air-lift reactor was assayed. Achieved yields, 53 % FOS of total sugars for at least 9 h, indicated the great suitability of the obtained immobilized preparation for application in large-scale systems.", publisher = "Korean Society of Industrial Engineering Chemistry", journal = "Journal of Industrial and Engineering Chemistry", title = "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109", pages = "156-149", volume = "117", doi = "10.1016/j.jiec.2022.09.051" }
Veljković, M., Stepanović, R., Banjanac, K., Ćorović, M., Milivojević, A., Simović, M., Milivojević, M.,& Bezbradica, D.. (2023). Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry Korean Society of Industrial Engineering Chemistry., 117, 149-156. https://doi.org/10.1016/j.jiec.2022.09.051
Veljković M, Stepanović R, Banjanac K, Ćorović M, Milivojević A, Simović M, Milivojević M, Bezbradica D. Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109. in Journal of Industrial and Engineering Chemistry. 2023;117:149-156. doi:10.1016/j.jiec.2022.09.051 .
Veljković, Milica, Stepanović, Relja, Banjanac, Katarina, Ćorović, Marija, Milivojević, Ana, Simović, Milica, Milivojević, Milan, Bezbradica, Dejan, "Continuous production of fructo-oligosaccharides using selectively immobilized fructosyltransferase from Aspergillus aculeatus onto Purolite® A109" in Journal of Industrial and Engineering Chemistry, 117 (2023):149-156, https://doi.org/10.1016/j.jiec.2022.09.051 . .