TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Balanč, Bojana
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Poklar-Ulrih, Nataša
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4302
AB  - The present study provides insight into changes in the liposomal membrane fluidity as a result of variation of structural components (two types of standard phospholipids - 1,2-dipalmitoyl-sn-glycerero3-phosphocholine, i.e. DPPC and 1-palmitoyl-2-oleoylglycerol-3-phosphocholine, i.e. POPC, one type of phospholipid mixture - Lipoid H and sterols - cholesterol and b-sitosterol), as well as variation in sterol concentration (5-50%). Fluorescence spectroscopy (anisotropy, r) has shown that DPPC liposomal membranes were more rigid compared to POPC and Lipoid H lipid bilayers. Furthermore, the increment of sterol concentration has caused a decrease in the membrane fluidity in all liposomal samples. On the other hand, phospholipid bilayers containing b-sitosterol has possessed better fluidity in comparison to their cholesterol parallels. Higher permeability of b-sitosterol liposomal membranes, as well as the beneficial effects of phytosterol on human health, favor the use of b-sitosterol in the preparation of liposomes as components of food, pharmaceutical and cosmetic products.
AB  - Prikazana studija daje uvid u promene fluidnosti lipozomalne membrane koje nastaju kao posledica promene strukturnih komponenata lipozoma (dve vrste standardnih fosfolipida - 1,2-dipalmitoil-snglicero-3-fosfoholin, odnosno DPPC i 1-palmitoil-2-oleoil-sn-glicero-3-fosfoholin, odnosno POPC, jedne vrste komercijalne fosfolipidne smeše - Lipoid H i sterola - holesterol i b-sitosterol), kao i promene udela sterola (0-50 mol %). Fluorimetrijska spektrofotometrija (anizotropija, r) pokazala je da su DPPC lipozomalne membrane bile rigidnije u poređenju sa POPC i Lipoid H lipidnim dvoslojima. Pored toga, povećenje koncentracije sterola prouzrokovalo je smanjenje permeabilnosti membrane u svim lipozomima. Sa druge strane, fosfolipidni dvosloji koji su sadržali b-sitosterol pokazali su veću fluidnost u poređenju sa njihovim paralelama sa holesterolom. Bolja permeabilnost b-sitosterol lipozomalnih membrana, kao i blagotvorni uticaji fitosterola na zdravlje ljudi favorizuju primenu b-sitosterola u pripremi lipozoma kao sastavnih delova prehrambenih, farmaceutskih i kozmetičkih proizvoda.
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika
T1  - Fluorescence analysis of liposomal membranes permeability
T1  - Fluorimetrijska analiza permeabilnosti lipozomalnih membrana
EP  - 498
IS  - 4
SP  - 493
VL  - 74
DO  - 10.5937/tehnika1904493J
ER  - 

@article{
author = "Jovanović, Aleksandra and Balanč, Bojana and Đorđević, Verica and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko and Poklar-Ulrih, Nataša",
year = "2019",
abstract = "The present study provides insight into changes in the liposomal membrane fluidity as a result of variation of structural components (two types of standard phospholipids - 1,2-dipalmitoyl-sn-glycerero3-phosphocholine, i.e. DPPC and 1-palmitoyl-2-oleoylglycerol-3-phosphocholine, i.e. POPC, one type of phospholipid mixture - Lipoid H and sterols - cholesterol and b-sitosterol), as well as variation in sterol concentration (5-50%). Fluorescence spectroscopy (anisotropy, r) has shown that DPPC liposomal membranes were more rigid compared to POPC and Lipoid H lipid bilayers. Furthermore, the increment of sterol concentration has caused a decrease in the membrane fluidity in all liposomal samples. On the other hand, phospholipid bilayers containing b-sitosterol has possessed better fluidity in comparison to their cholesterol parallels. Higher permeability of b-sitosterol liposomal membranes, as well as the beneficial effects of phytosterol on human health, favor the use of b-sitosterol in the preparation of liposomes as components of food, pharmaceutical and cosmetic products., Prikazana studija daje uvid u promene fluidnosti lipozomalne membrane koje nastaju kao posledica promene strukturnih komponenata lipozoma (dve vrste standardnih fosfolipida - 1,2-dipalmitoil-snglicero-3-fosfoholin, odnosno DPPC i 1-palmitoil-2-oleoil-sn-glicero-3-fosfoholin, odnosno POPC, jedne vrste komercijalne fosfolipidne smeše - Lipoid H i sterola - holesterol i b-sitosterol), kao i promene udela sterola (0-50 mol %). Fluorimetrijska spektrofotometrija (anizotropija, r) pokazala je da su DPPC lipozomalne membrane bile rigidnije u poređenju sa POPC i Lipoid H lipidnim dvoslojima. Pored toga, povećenje koncentracije sterola prouzrokovalo je smanjenje permeabilnosti membrane u svim lipozomima. Sa druge strane, fosfolipidni dvosloji koji su sadržali b-sitosterol pokazali su veću fluidnost u poređenju sa njihovim paralelama sa holesterolom. Bolja permeabilnost b-sitosterol lipozomalnih membrana, kao i blagotvorni uticaji fitosterola na zdravlje ljudi favorizuju primenu b-sitosterola u pripremi lipozoma kao sastavnih delova prehrambenih, farmaceutskih i kozmetičkih proizvoda.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika",
title = "Fluorescence analysis of liposomal membranes permeability, Fluorimetrijska analiza permeabilnosti lipozomalnih membrana",
pages = "498-493",
number = "4",
volume = "74",
doi = "10.5937/tehnika1904493J"
}

Jovanović, A., Balanč, B., Đorđević, V., Šavikin, K., Nedović, V., Bugarski, B.,& Poklar-Ulrih, N.. (2019). Fluorescence analysis of liposomal membranes permeability. in Tehnika
Savez inženjera i tehničara Srbije, Beograd., 74(4), 493-498.
https://doi.org/10.5937/tehnika1904493J

Jovanović A, Balanč B, Đorđević V, Šavikin K, Nedović V, Bugarski B, Poklar-Ulrih N. Fluorescence analysis of liposomal membranes permeability. in Tehnika. 2019;74(4):493-498.
doi:10.5937/tehnika1904493J .

Jovanović, Aleksandra, Balanč, Bojana, Đorđević, Verica, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, Poklar-Ulrih, Nataša, "Fluorescence analysis of liposomal membranes permeability" in Tehnika, 74, no. 4 (2019):493-498,
https://doi.org/10.5937/tehnika1904493J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Pravilović, Radoslava
AU  - Ota, Ajda
AU  - Poklar-Ulrih, Nataša
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6447
AB  - Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0-
50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks
(PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i
upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem
polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C,
upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da
povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja
fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona
spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala
kontinuirano povećanje veličine čestica sa porastom koncentracije
holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog
rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice
su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema
dobijenim vrednostima PDI, samo SUV bez holesterola i sa 5, 30 i 40 mol %
holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2).
Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su
negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa
50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje
lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %.
AB  - The aim of this study was examination of the influence of various cholesterol
content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index
(PDI) and zeta potential of liposomes prepared using the thin film method
and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization
(p) and anisotropy (r) measurements, within the temperature range of 20 to
60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have
shown that increasing the cholesterol content in the liposomes decreased the
fluidity and enhanced the rigidity of liposomal membranes. The photon
correlation spectroscopy data have shown that the size of small unilamellar
vesicles (SUV) increased continuously with the increase in cholesterol
concentration, while multilamellar vesicles (MLV) did not show similar
trend. In both cases, significantly larger vesicles were obtained using the
highest content of cholesterol (50 mol %). According to the PDI values, only
SUV without cholesterol and with 5, 30 and 40 mol % of cholesterol exhibit
narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential,
as an important parameter of system stability, were negative in all samples,
while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile
liposomes and an appropriate fluidity of the membrane has been 50 mol %.
PB  - Faculty of Technology, Zvornik
C3  - V International Congress: „Engineering, Environment and Materials in Processing Industry“
T1  - Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal
EP  - 1514
SP  - 1502
DO  - 10.7251/EEMSR15011502J
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Pravilović, Radoslava and Ota, Ajda and Poklar-Ulrih, Nataša and Nedović, Viktor and Bugarski, Branko",
year = "2017",
abstract = "Cilj ove studije bilo je ispitivanje uticaja različitih sadržaja holesterola (0-
50 mol %) na fluidnost membrana, veličinu čestica, polidisperzni indeks
(PDI) i zeta potencijal lipozoma, pripremljenih metodom tankog filma i
upotrebom fosfatidilholina soje, Phospholipon 90 (Ph). Merenjem
polarizacije (p) i anizotropije (r), u temperaturnom intervalu od 20 do 60˚C,
upotrebom fluorofore 1,6-difenil-1,3,5-heksatrien (DPH), pokazano je da
povećanjem koncentracije holesterola u lipozomima dolazi do smanjenja
fluidnosti i povećanja rigidnosti membrane lipozoma. Foton korelaciona
spektroskopija je kod malih unilamelarnih lipozoma (SUV) pokazala
kontinuirano povećanje veličine čestica sa porastom koncentracije
holesterola, dok kod multilamelarnih lipozoma (MLV) trend kontinuiranog
rasta čestica nije primećen. U oba slučaja, statistički značajno veće čestice
su dobijene sa najvišom koncentracijom holesterola (50 mol %). Prema
dobijenim vrednostima PDI, samo SUV bez holesterola i sa 5, 30 i 40 mol %
holesterola, poseduju usku raspodelu čestica po veličini (PDI manji od 0,2).
Vrednosti zeta potencijala, kao važnog parametra stabilnosti sistema, bile su
negativne u svim uzorcima, dok su najviše apsolutne vrednosti dostignute sa
50 mol % holesterola. Optimalna koncentracija holesterola za dobijanje
lipozoma odgovarajuće stabilnosti i fluidnosti membrana jeste 50 mol %., The aim of this study was examination of the influence of various cholesterol
content (0-50 mol %) on membrane fluidity, vesicle size, polydispersity index
(PDI) and zeta potential of liposomes prepared using the thin film method
and phosphatidylcholine from soybean, Phospholipon 90 (Ph). Polarization
(p) and anisotropy (r) measurements, within the temperature range of 20 to
60˚C, using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH), have
shown that increasing the cholesterol content in the liposomes decreased the
fluidity and enhanced the rigidity of liposomal membranes. The photon
correlation spectroscopy data have shown that the size of small unilamellar
vesicles (SUV) increased continuously with the increase in cholesterol
concentration, while multilamellar vesicles (MLV) did not show similar
trend. In both cases, significantly larger vesicles were obtained using the
highest content of cholesterol (50 mol %). According to the PDI values, only
SUV without cholesterol and with 5, 30 and 40 mol % of cholesterol exhibit
narrow size distribution (PDI < 0,2). Furthermore, values of zeta potential,
as an important parameter of system stability, were negative in all samples,
while the highest absolute values were achieved with 50 mol % of cholesterol. The optimal concentration of cholesterol to obtain stabile
liposomes and an appropriate fluidity of the membrane has been 50 mol %.",
publisher = "Faculty of Technology, Zvornik",
journal = "V International Congress: „Engineering, Environment and Materials in Processing Industry“",
title = "Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal",
pages = "1514-1502",
doi = "10.7251/EEMSR15011502J"
}

Jovanović, A. A., Balanč, B., Pravilović, R., Ota, A., Poklar-Ulrih, N., Nedović, V.,& Bugarski, B.. (2017). Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal. in V International Congress: „Engineering, Environment and Materials in Processing Industry“
Faculty of Technology, Zvornik., 1502-1514.
https://doi.org/10.7251/EEMSR15011502J

Jovanović AA, Balanč B, Pravilović R, Ota A, Poklar-Ulrih N, Nedović V, Bugarski B. Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal. in V International Congress: „Engineering, Environment and Materials in Processing Industry“. 2017;:1502-1514.
doi:10.7251/EEMSR15011502J .

Jovanović, Aleksandra A., Balanč, Bojana, Pravilović, Radoslava, Ota, Ajda, Poklar-Ulrih, Nataša, Nedović, Viktor, Bugarski, Branko, "Uticaj holesterola na fluidnost membrane lipozoma, veličinu lipozomnih čestica i njihov zeta potencijal" in V International Congress: „Engineering, Environment and Materials in Processing Industry“ (2017):1502-1514,
https://doi.org/10.7251/EEMSR15011502J . .