TY  - CONF
AU  - Knežević-Jugović, Zorica
AU  - Jugović, Branimir
AU  - Jakovetić, Sonja
AU  - Bezbradica, Dejan
AU  - Antov, Mirjana
AU  - Saied, Omar Ali
AU  - Gvozdenović, Milica
PY  - 2011
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1768
AB  - The present study compares the results of two different methods employed for preparation of polyaniline based glucose biosensor with respect to enzyme loading, biosensing efficiency and potential stability. Kinetic analysis of the potentiometric data for two enzyme immobilized electrode systems show that the GOx/PANI electrode is suitable for assaying samples with low analyte concentrations, whereas the GOx/m-ABA/PANI electrode system exhibits a better potential stability. It may therefore be possible to achieve high level of biosensing efficiency by chemical modeling and synthesis combined with careful selection of the immobilization method.
PB  - Tatranské Matliare : Slovak Society of Chemical Engineering
C3  - Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering
T1  - Design of a polyaniline based biosensor electrode for glucose: A comparative study of two immobilized systems
EP  - 1525
SP  - 1519
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1768
ER  - 

@conference{
author = "Knežević-Jugović, Zorica and Jugović, Branimir and Jakovetić, Sonja and Bezbradica, Dejan and Antov, Mirjana and Saied, Omar Ali and Gvozdenović, Milica",
year = "2011",
abstract = "The present study compares the results of two different methods employed for preparation of polyaniline based glucose biosensor with respect to enzyme loading, biosensing efficiency and potential stability. Kinetic analysis of the potentiometric data for two enzyme immobilized electrode systems show that the GOx/PANI electrode is suitable for assaying samples with low analyte concentrations, whereas the GOx/m-ABA/PANI electrode system exhibits a better potential stability. It may therefore be possible to achieve high level of biosensing efficiency by chemical modeling and synthesis combined with careful selection of the immobilization method.",
publisher = "Tatranské Matliare : Slovak Society of Chemical Engineering",
journal = "Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering",
title = "Design of a polyaniline based biosensor electrode for glucose: A comparative study of two immobilized systems",
pages = "1525-1519",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1768"
}

Knežević-Jugović, Z., Jugović, B., Jakovetić, S., Bezbradica, D., Antov, M., Saied, O. A.,& Gvozdenović, M.. (2011). Design of a polyaniline based biosensor electrode for glucose: A comparative study of two immobilized systems. in Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering
Tatranské Matliare : Slovak Society of Chemical Engineering., 1519-1525.
https://hdl.handle.net/21.15107/rcub_technorep_1768

Knežević-Jugović Z, Jugović B, Jakovetić S, Bezbradica D, Antov M, Saied OA, Gvozdenović M. Design of a polyaniline based biosensor electrode for glucose: A comparative study of two immobilized systems. in Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering. 2011;:1519-1525.
https://hdl.handle.net/21.15107/rcub_technorep_1768 .

Knežević-Jugović, Zorica, Jugović, Branimir, Jakovetić, Sonja, Bezbradica, Dejan, Antov, Mirjana, Saied, Omar Ali, Gvozdenović, Milica, "Design of a polyaniline based biosensor electrode for glucose: A comparative study of two immobilized systems" in Proceedings of the 38th International Conference of Slovak Society of Chemical Engineering (2011):1519-1525,
https://hdl.handle.net/21.15107/rcub_technorep_1768 .

TY  - JOUR
AU  - Šaponjić, Svetlana V.
AU  - Knežević-Jugović, Zorica
AU  - Bezbradica, Dejan
AU  - Žuža, Milena
AU  - Saied, Omar Ali
AU  - Bošković-Vragolović, Nevenka
AU  - Mijin, Dušan
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1691
AB  - Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion ( gt  99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g(-1) dry support and in a relatively short time (24 hrs) at 37 degrees C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale.
PB  - University of Catolica De Valparaiso, Valparaiso
T2  - Electronic Journal of Biotechnology
T1  - Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study
IS  - 6
VL  - 13
DO  - 10.2225/vol13-issue6-fulltext-8
ER  - 

@article{
author = "Šaponjić, Svetlana V. and Knežević-Jugović, Zorica and Bezbradica, Dejan and Žuža, Milena and Saied, Omar Ali and Bošković-Vragolović, Nevenka and Mijin, Dušan",
year = "2010",
abstract = "Lipase from Candida rugosa was covalently immobilized on Sepabeads EC-EP for application for amyl caprylate synthesis in an organic solvent system. Several solvents were tested in terms of biocatalyst stability and the best result was obtained with isooctane. The lipase-catalyzed esterification in the selected system was performed in batch and fluidized bed reactor systems. The influence of several important reaction parameters including temperature, initial water content, enzyme loading, acid/alcohol molar ratio, and time of addition of molecular sieves is carefully analyzed by means of an experimental design. Almost complete conversion ( gt  99%) of the substrate to ester could be performed in a batch reactor system, using lipase loading as low as 37 mg g(-1) dry support and in a relatively short time (24 hrs) at 37 degrees C, when high initial substrate molar ratio of 2.2 is used. Kinetics in a fluidized bed reactor system seems to still have a slightly better profile than in the batch system (90.2% yields after 14 hrs). The fluidized bed reactor operated for up 70 hrs almost with no loss in productivity, implying that the proposed process and the immobilized system could provide a promising approach for the amyl caprylate synthesis at the industrial scale.",
publisher = "University of Catolica De Valparaiso, Valparaiso",
journal = "Electronic Journal of Biotechnology",
title = "Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study",
number = "6",
volume = "13",
doi = "10.2225/vol13-issue6-fulltext-8"
}

Šaponjić, S. V., Knežević-Jugović, Z., Bezbradica, D., Žuža, M., Saied, O. A., Bošković-Vragolović, N.,& Mijin, D.. (2010). Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study. in Electronic Journal of Biotechnology
University of Catolica De Valparaiso, Valparaiso., 13(6).
https://doi.org/10.2225/vol13-issue6-fulltext-8

Šaponjić SV, Knežević-Jugović Z, Bezbradica D, Žuža M, Saied OA, Bošković-Vragolović N, Mijin D. Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study. in Electronic Journal of Biotechnology. 2010;13(6).
doi:10.2225/vol13-issue6-fulltext-8 .

Šaponjić, Svetlana V., Knežević-Jugović, Zorica, Bezbradica, Dejan, Žuža, Milena, Saied, Omar Ali, Bošković-Vragolović, Nevenka, Mijin, Dušan, "Use of Candida rugosa lipase immobilized on sepabeads for the amyl caprylate synthesis: Batch and fluidized bed reactor study" in Electronic Journal of Biotechnology, 13, no. 6 (2010),
https://doi.org/10.2225/vol13-issue6-fulltext-8 . .