TY  - JOUR
AU  - Radulović, Zorica
AU  - Mirković, Nemanja
AU  - Bogović-Matijašič, Bojana
AU  - Petrušić, Milica M.
AU  - Petrović, Tanja
AU  - Manojlović, Verica
AU  - Nedović, Viktor
PY  - 2012
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5734
AB  - The basic requirement for probiotic bacteria to be able to perform expected positive effects is to be alive. Therefore, appropriate quantification methods are crucial. Bacterial quantification based on nucleic acid detection is increasingly used. Spray-drying (SD) is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to investigate the survival of spray-dried Lactobacillus plantarum 564 and Lactobacillus paracasei Z-8, and to investigate the impact on some probiotic properties caused by SD of both tested strains. Besides the plate count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (PCR) for determining spray-dried tested strains. The number of intact cells, Lb. plantarum 564 and Lb. paracasei Z-8, was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by the plate count method. Spray-dried Lb. plantarum 564 and Lb. paracasei Z-8 demonstrated very good probiotic ability. It may be concluded that the PMA real-time PCR determination of the viability of probiotic bacteria could complement the plate count method and SD may be a cost-effective way to produce large quantities of some probiotic cultures.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR
EP  - 1472
IS  - 4
SP  - 1465
VL  - 64
DO  - 10.2298/ABS1204465R
ER  - 

@article{
author = "Radulović, Zorica and Mirković, Nemanja and Bogović-Matijašič, Bojana and Petrušić, Milica M. and Petrović, Tanja and Manojlović, Verica and Nedović, Viktor",
year = "2012",
abstract = "The basic requirement for probiotic bacteria to be able to perform expected positive effects is to be alive. Therefore, appropriate quantification methods are crucial. Bacterial quantification based on nucleic acid detection is increasingly used. Spray-drying (SD) is one of the possibilities to improve the survival of probiotic bacteria against negative environmental effects. The aim of this study was to investigate the survival of spray-dried Lactobacillus plantarum 564 and Lactobacillus paracasei Z-8, and to investigate the impact on some probiotic properties caused by SD of both tested strains. Besides the plate count technique, the aim was to examine the possibility of using propidium monoazide (PMA) in combination with real-time polymerase chain reaction (PCR) for determining spray-dried tested strains. The number of intact cells, Lb. plantarum 564 and Lb. paracasei Z-8, was determined by real-time PCR with PMA, and it was similar to the number of investigated strains obtained by the plate count method. Spray-dried Lb. plantarum 564 and Lb. paracasei Z-8 demonstrated very good probiotic ability. It may be concluded that the PMA real-time PCR determination of the viability of probiotic bacteria could complement the plate count method and SD may be a cost-effective way to produce large quantities of some probiotic cultures.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR",
pages = "1472-1465",
number = "4",
volume = "64",
doi = "10.2298/ABS1204465R"
}

Radulović, Z., Mirković, N., Bogović-Matijašič, B., Petrušić, M. M., Petrović, T., Manojlović, V.,& Nedović, V.. (2012). Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1465-1472.
https://doi.org/10.2298/ABS1204465R

Radulović Z, Mirković N, Bogović-Matijašič B, Petrušić MM, Petrović T, Manojlović V, Nedović V. Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR. in Archives of Biological Sciences. 2012;64(4):1465-1472.
doi:10.2298/ABS1204465R .

Radulović, Zorica, Mirković, Nemanja, Bogović-Matijašič, Bojana, Petrušić, Milica M., Petrović, Tanja, Manojlović, Verica, Nedović, Viktor, "Quantification of viable spray-dried potential probiotic lactobacilli using real-time PCR" in Archives of Biological Sciences, 64, no. 4 (2012):1465-1472,
https://doi.org/10.2298/ABS1204465R . .

TY  - JOUR
AU  - Trifković, Branka
AU  - Todorović, Aleksandar
AU  - Lazić, Vojkan
AU  - Draganjac, Miroslav
AU  - Mirković, Nemanja
AU  - Jokić, Bojan
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5632
AB  - Background/Aim. One of the results of many years of Cerec® 3D CAD/CAM system technological development is implementation of one intraoral and two extraoral optical scanning methods which, depending on the current indications, are applied in making fixed restorations. The aim of this study was to determine the degree of precision of optical scanning methods by the use of the Cerec®3D CAD/CAM system in the process of making ceramic inlays. Methods. The study was conducted in three experimental groups of inlays prepared using the procedure of three methods of scanning Cerec ®3D system. Ceramic inlays made by conventional methodology were the control group. The accuracy of optical scanning methods of the Cerec®3D system computer aided designcomputer aided manufacturing (CAD/CAM) was indirectly examined by measuring a marginal gap size between inlays and demarcation preparation by scanning electron microscope (SEM). Results. The results of the study showed a difference in the accuracy of the existing methods of scanning dental CAD/CAM systems. The highest level of accuracy was achieved by the extraoral optical superficial scanning technique. The value of marginal gap size inlays made with the technique of extraoral optical superficial scanning was 32.97 ± 13.17 μ. Techniques of intraoral optical superficial and extraoral point laser scanning showed a lower level of accuracy (40.29 ± 21.46 μ for inlays of intraoral optical superficial scanning and 99.67 ± 37.25 μ for inlays of extraoral point laser scanning). Conclusion. Optical scanning methods in dental CAM/CAM technologies are precise methods of digitizing the spatial models; application of extraoral optical scanning methods provides the hightest precision.
AB  - Uvod/Cilj. Jedan od rezultata dugogodišnjeg tehnološkog usavršavanja Cerec®3D sistema jeste implementacija jednog intraoralnog i dva ekstraoralna metoda optičkog skeniranja koji se, u zavisnosti od postojeće indikacije, primenjuju u izradi fiksnih nadoknada. Cilj rada bio je da se utvrdi stepen preciznosti optičkih metoda skeniranja Cerec®3D sistema u postupku izrade keramičkih inleja. Metode. Ispitivanje je sprovedeno u okviru tri eksperimentalne grupe inleja u čijoj izradi su primenjena tri postupka skeniranja Cerec®3D sistema. Keramički inleji izrađeni konvencionalnom metodologijom predstavljali su kontrolnu grupu. Preciznost optičkih metoda skeniranja Cerec®3D sistema ispitivana je indirektno, merenjem veličine rubnog zaptivanja između inleja i demarkacije preparacije pomoću skenirajućeg elektronskog mikroskopa (SEM). Rezultati. Istraživanjem je ustanovljeno da postoji razlika u preciznosti postojećih metoda skeniranja stomatoloških Computer aided design-computer aided manufacturing (CAD/CAM) tehnologija. Najviši stepen preciznosti pokazala je tehnika optičkog ekstraoralnog površinskog skeniranja. Vrednost rubnog zaptivanja inleja izrađenih primenom ove tehnike iznosila je 32,97 ± 13,17 μ. Tehnike intraoralnog optičkog površinskog i ekstraoralnog tačkastog skeniranja pokazuju niži nivo preciznosti (40,29 ± 21,46 μ za inleje intraoralnog optičkog površinskog i 99,67 ± 37,25 μ za inleje ekstraoralnog tačkastog skeniranja). Zaključak. Optičke metode skeniranja u stomatološkim CAD/CAM tehnologijama predstavljaju precizne metode prostorne digitalizacije modela; pri tom, najveću preciznost pruža primena ekstraoralnih optičkih metoda skeniranja.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Accuracy of optical scanning methods of the Cerec®3D system in the process of making ceramic inlays
T1  - Preciznost optičkih metoda skeniranja Cerec®3D sistema u postupku izrade keramičkih inleja
EP  - 818
IS  - 10
SP  - 812
VL  - 67
DO  - 10.2298/VSP1010812T
ER  - 

@article{
author = "Trifković, Branka and Todorović, Aleksandar and Lazić, Vojkan and Draganjac, Miroslav and Mirković, Nemanja and Jokić, Bojan",
year = "2010",
abstract = "Background/Aim. One of the results of many years of Cerec® 3D CAD/CAM system technological development is implementation of one intraoral and two extraoral optical scanning methods which, depending on the current indications, are applied in making fixed restorations. The aim of this study was to determine the degree of precision of optical scanning methods by the use of the Cerec®3D CAD/CAM system in the process of making ceramic inlays. Methods. The study was conducted in three experimental groups of inlays prepared using the procedure of three methods of scanning Cerec ®3D system. Ceramic inlays made by conventional methodology were the control group. The accuracy of optical scanning methods of the Cerec®3D system computer aided designcomputer aided manufacturing (CAD/CAM) was indirectly examined by measuring a marginal gap size between inlays and demarcation preparation by scanning electron microscope (SEM). Results. The results of the study showed a difference in the accuracy of the existing methods of scanning dental CAD/CAM systems. The highest level of accuracy was achieved by the extraoral optical superficial scanning technique. The value of marginal gap size inlays made with the technique of extraoral optical superficial scanning was 32.97 ± 13.17 μ. Techniques of intraoral optical superficial and extraoral point laser scanning showed a lower level of accuracy (40.29 ± 21.46 μ for inlays of intraoral optical superficial scanning and 99.67 ± 37.25 μ for inlays of extraoral point laser scanning). Conclusion. Optical scanning methods in dental CAM/CAM technologies are precise methods of digitizing the spatial models; application of extraoral optical scanning methods provides the hightest precision., Uvod/Cilj. Jedan od rezultata dugogodišnjeg tehnološkog usavršavanja Cerec®3D sistema jeste implementacija jednog intraoralnog i dva ekstraoralna metoda optičkog skeniranja koji se, u zavisnosti od postojeće indikacije, primenjuju u izradi fiksnih nadoknada. Cilj rada bio je da se utvrdi stepen preciznosti optičkih metoda skeniranja Cerec®3D sistema u postupku izrade keramičkih inleja. Metode. Ispitivanje je sprovedeno u okviru tri eksperimentalne grupe inleja u čijoj izradi su primenjena tri postupka skeniranja Cerec®3D sistema. Keramički inleji izrađeni konvencionalnom metodologijom predstavljali su kontrolnu grupu. Preciznost optičkih metoda skeniranja Cerec®3D sistema ispitivana je indirektno, merenjem veličine rubnog zaptivanja između inleja i demarkacije preparacije pomoću skenirajućeg elektronskog mikroskopa (SEM). Rezultati. Istraživanjem je ustanovljeno da postoji razlika u preciznosti postojećih metoda skeniranja stomatoloških Computer aided design-computer aided manufacturing (CAD/CAM) tehnologija. Najviši stepen preciznosti pokazala je tehnika optičkog ekstraoralnog površinskog skeniranja. Vrednost rubnog zaptivanja inleja izrađenih primenom ove tehnike iznosila je 32,97 ± 13,17 μ. Tehnike intraoralnog optičkog površinskog i ekstraoralnog tačkastog skeniranja pokazuju niži nivo preciznosti (40,29 ± 21,46 μ za inleje intraoralnog optičkog površinskog i 99,67 ± 37,25 μ za inleje ekstraoralnog tačkastog skeniranja). Zaključak. Optičke metode skeniranja u stomatološkim CAD/CAM tehnologijama predstavljaju precizne metode prostorne digitalizacije modela; pri tom, najveću preciznost pruža primena ekstraoralnih optičkih metoda skeniranja.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Accuracy of optical scanning methods of the Cerec®3D system in the process of making ceramic inlays, Preciznost optičkih metoda skeniranja Cerec®3D sistema u postupku izrade keramičkih inleja",
pages = "818-812",
number = "10",
volume = "67",
doi = "10.2298/VSP1010812T"
}

Trifković, B., Todorović, A., Lazić, V., Draganjac, M., Mirković, N.,& Jokić, B.. (2010). Accuracy of optical scanning methods of the Cerec®3D system in the process of making ceramic inlays. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 67(10), 812-818.
https://doi.org/10.2298/VSP1010812T

Trifković B, Todorović A, Lazić V, Draganjac M, Mirković N, Jokić B. Accuracy of optical scanning methods of the Cerec®3D system in the process of making ceramic inlays. in Vojnosanitetski pregled. 2010;67(10):812-818.
doi:10.2298/VSP1010812T .

Trifković, Branka, Todorović, Aleksandar, Lazić, Vojkan, Draganjac, Miroslav, Mirković, Nemanja, Jokić, Bojan, "Accuracy of optical scanning methods of the Cerec®3D system in the process of making ceramic inlays" in Vojnosanitetski pregled, 67, no. 10 (2010):812-818,
https://doi.org/10.2298/VSP1010812T . .

TY  - JOUR
AU  - Radulović, Zorica
AU  - Petrović, Tanja
AU  - Nedović, Viktor
AU  - Dimitrijević, Suzana
AU  - Mirković, Nemanja
AU  - Petrusić, Milica
AU  - Paunović, Dušanka
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1546
AB  - Lactic acid bacteria strains isolated from traditional made cheeses constitute a reservoir of unexplored potential in biotechnology. In this study four autochthonous lactobacilli strains, isolated from traditional white brined cheeses and identified as Lactobacillus paracasei (08, 564, 05 and 02), were investigated on potential probiotic ability. The investigation comprised sensitivity to simulated gastrointestinal tract conditions, antimicrobial activity against wide range of pathogens, antibiotic resistance as well as autoaggregation ability. Lactobacillus rhamnosus GG was used as referent strain. Three tested strains grew well in simulated gastrointestinal conditions, but their sensitivity was greater on bile acids and pancreatin compared with pepsin low pH 2.5. The examined strains had different sensitivity to antibiotics, but three strains showed very good antimicrobial activity to pathogens. All strains demonstrated very good autoaggregation ability. For three of four examined strains of Lb. paracasei probiotic potential was similar with referent strain Lb. rhamnosus GG, determined in vitro.
PB  - Croatian Dairy Union
T2  - Mljekarstvo
T1  - Characterization of autochthonous Lactobacillus paracasei strains on potential probiotic ability
EP  - 93
IS  - 2
SP  - 86
VL  - 60
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1546
ER  - 

@article{
author = "Radulović, Zorica and Petrović, Tanja and Nedović, Viktor and Dimitrijević, Suzana and Mirković, Nemanja and Petrusić, Milica and Paunović, Dušanka",
year = "2010",
abstract = "Lactic acid bacteria strains isolated from traditional made cheeses constitute a reservoir of unexplored potential in biotechnology. In this study four autochthonous lactobacilli strains, isolated from traditional white brined cheeses and identified as Lactobacillus paracasei (08, 564, 05 and 02), were investigated on potential probiotic ability. The investigation comprised sensitivity to simulated gastrointestinal tract conditions, antimicrobial activity against wide range of pathogens, antibiotic resistance as well as autoaggregation ability. Lactobacillus rhamnosus GG was used as referent strain. Three tested strains grew well in simulated gastrointestinal conditions, but their sensitivity was greater on bile acids and pancreatin compared with pepsin low pH 2.5. The examined strains had different sensitivity to antibiotics, but three strains showed very good antimicrobial activity to pathogens. All strains demonstrated very good autoaggregation ability. For three of four examined strains of Lb. paracasei probiotic potential was similar with referent strain Lb. rhamnosus GG, determined in vitro.",
publisher = "Croatian Dairy Union",
journal = "Mljekarstvo",
title = "Characterization of autochthonous Lactobacillus paracasei strains on potential probiotic ability",
pages = "93-86",
number = "2",
volume = "60",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1546"
}

Radulović, Z., Petrović, T., Nedović, V., Dimitrijević, S., Mirković, N., Petrusić, M.,& Paunović, D.. (2010). Characterization of autochthonous Lactobacillus paracasei strains on potential probiotic ability. in Mljekarstvo
Croatian Dairy Union., 60(2), 86-93.
https://hdl.handle.net/21.15107/rcub_technorep_1546

Radulović Z, Petrović T, Nedović V, Dimitrijević S, Mirković N, Petrusić M, Paunović D. Characterization of autochthonous Lactobacillus paracasei strains on potential probiotic ability. in Mljekarstvo. 2010;60(2):86-93.
https://hdl.handle.net/21.15107/rcub_technorep_1546 .

Radulović, Zorica, Petrović, Tanja, Nedović, Viktor, Dimitrijević, Suzana, Mirković, Nemanja, Petrusić, Milica, Paunović, Dušanka, "Characterization of autochthonous Lactobacillus paracasei strains on potential probiotic ability" in Mljekarstvo, 60, no. 2 (2010):86-93,
https://hdl.handle.net/21.15107/rcub_technorep_1546 .