TY  - JOUR
AU  - Perović, Milica N.
AU  - Knežević Jugović, Zorica D.
AU  - Antov, Mirjana G.
PY  - 2024-06
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7257
AB  - Nanoparticles  prepared by heat treatment of protein from pumpkin leaves were evaluated as potential carriers of β-carotene. White protein fraction was recovered from green juice produced by pressing the leaves, with the step of enzyme-assisted extraction (green protocol) or without it (conventional protocol). Heat treatment of white protein fractions from conventional and green protocols at 90 °C and pH 9.3 during 20 min induced formation of nanoparticles with peak diameter 18 nm and 21 nm, respectively. Due to heating, portion of β-sheets in nanoparticles from both native protein fractions decreased by approximately 15 %, associated with an increase in surface hydrophobicity-to-area ratio. Quenching constant of β-carotene for nanoparticles was increased nearly 100 times by heating the white protein fraction recovered in green protocol. Native white protein fraction from conventional protocol and corresponding nanoparticles exhibited high ability to bind β-carotene, with quenching constant 3 × 105 L/mol and 3.3 × 105 L/mol, respectively. White protein fraction from pumpkin leaves appeared to be a suitable substrate for the fabrication of nanoparticles by heat treatment, with potential application as β-carotene nanocarriers in food matrices.
PB  - Elsevier B.V.
T2  - Future Foods
T1  - Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers
SP  - 100310
VL  - 9
DO  - 10.1016/j.fufo.2024.100310
ER  - 

@article{
author = "Perović, Milica N. and Knežević Jugović, Zorica D. and Antov, Mirjana G.",
year = "2024-06",
abstract = "Nanoparticles  prepared by heat treatment of protein from pumpkin leaves were evaluated as potential carriers of β-carotene. White protein fraction was recovered from green juice produced by pressing the leaves, with the step of enzyme-assisted extraction (green protocol) or without it (conventional protocol). Heat treatment of white protein fractions from conventional and green protocols at 90 °C and pH 9.3 during 20 min induced formation of nanoparticles with peak diameter 18 nm and 21 nm, respectively. Due to heating, portion of β-sheets in nanoparticles from both native protein fractions decreased by approximately 15 %, associated with an increase in surface hydrophobicity-to-area ratio. Quenching constant of β-carotene for nanoparticles was increased nearly 100 times by heating the white protein fraction recovered in green protocol. Native white protein fraction from conventional protocol and corresponding nanoparticles exhibited high ability to bind β-carotene, with quenching constant 3 × 105 L/mol and 3.3 × 105 L/mol, respectively. White protein fraction from pumpkin leaves appeared to be a suitable substrate for the fabrication of nanoparticles by heat treatment, with potential application as β-carotene nanocarriers in food matrices.",
publisher = "Elsevier B.V.",
journal = "Future Foods",
title = "Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers",
pages = "100310",
volume = "9",
doi = "10.1016/j.fufo.2024.100310"
}

Perović, M. N., Knežević Jugović, Z. D.,& Antov, M. G.. (2024-06). Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. in Future Foods
Elsevier B.V.., 9, 100310.
https://doi.org/10.1016/j.fufo.2024.100310

Perović MN, Knežević Jugović ZD, Antov MG. Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. in Future Foods. 2024;9:100310.
doi:10.1016/j.fufo.2024.100310 .

Perović, Milica N., Knežević Jugović, Zorica D., Antov, Mirjana G., "Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers" in Future Foods, 9 (2024-06):100310,
https://doi.org/10.1016/j.fufo.2024.100310 . .

TY  - JOUR
AU  - Kosić, Višnja
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Banković, Predrag
AU  - Jović-Jovičić, Nataša
AU  - Knežević-Jugović, Zorica
AU  - Milutinović-Nikolić, Aleksandra
PY  - 2024-03
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7279
AB  - Three glycoside hydrolases (α-amylase, xylanase and pullulanase) were immobilized on low-cost, environmentally friendly, easily modified clay rich in beidellite. Modifications included common procedures: Na-exchange, acid activation, pillaring, pillaring followed by acid activation, and organo-modifications with chitosan. Supports were characterized by chemical analysis, low temperature N2 physisorption, X-ray powder diffraction (XRPD) and Fourier-transform infrared (FT-IR) spectroscopy. The point of zero charge was also determined. Specific activity of different immobilizates of selected glycoside hydrolases was notably influenced by the type of chemical modification of supports. For each enzyme optimal support was chosen and storage stability was tested. α-Amylase immobilized on acid-activated support retained up to 95% of its initial specific activity of 105.6 ± 5.1 U g−1 after a testing period of 120 days. The most suitable support for xylanase was chitosan-modified beidellite with having specific activity of 90.0 ± 1.4 U g−1 which retained >50% its value after 120 days. Specific activity of pullulanase immobilized on pillared sample that was subsequently activated by acid was 44.5 ± 0.7 U g−1. Initial activity was preserved up to 33% for the same testing period. Comparing these results to the storage stability of the free enzymes that completely lost their activity for the longest period of 40 days, it can be concluded that appropriately modified beidellite- based clays could be used as suitable supports for stabilization of glycoside hydrolases. Nevertheless, further characterization of immobilizates (pH, thermal and operational stability) is needed in order to raise the suitability for larger scale processes in food industry.
PB  - Elsevier Ltd.
T2  - Applied Clay Science
T1  - Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite
SP  - 107289
VL  - 250
DO  - 10.1016/j.clay.2024.107289
ER  - 

@article{
author = "Kosić, Višnja and Božić, Nataša and Dojnov, Biljana and Banković, Predrag and Jović-Jovičić, Nataša and Knežević-Jugović, Zorica and Milutinović-Nikolić, Aleksandra",
year = "2024-03",
abstract = "Three glycoside hydrolases (α-amylase, xylanase and pullulanase) were immobilized on low-cost, environmentally friendly, easily modified clay rich in beidellite. Modifications included common procedures: Na-exchange, acid activation, pillaring, pillaring followed by acid activation, and organo-modifications with chitosan. Supports were characterized by chemical analysis, low temperature N2 physisorption, X-ray powder diffraction (XRPD) and Fourier-transform infrared (FT-IR) spectroscopy. The point of zero charge was also determined. Specific activity of different immobilizates of selected glycoside hydrolases was notably influenced by the type of chemical modification of supports. For each enzyme optimal support was chosen and storage stability was tested. α-Amylase immobilized on acid-activated support retained up to 95% of its initial specific activity of 105.6 ± 5.1 U g−1 after a testing period of 120 days. The most suitable support for xylanase was chitosan-modified beidellite with having specific activity of 90.0 ± 1.4 U g−1 which retained >50% its value after 120 days. Specific activity of pullulanase immobilized on pillared sample that was subsequently activated by acid was 44.5 ± 0.7 U g−1. Initial activity was preserved up to 33% for the same testing period. Comparing these results to the storage stability of the free enzymes that completely lost their activity for the longest period of 40 days, it can be concluded that appropriately modified beidellite- based clays could be used as suitable supports for stabilization of glycoside hydrolases. Nevertheless, further characterization of immobilizates (pH, thermal and operational stability) is needed in order to raise the suitability for larger scale processes in food industry.",
publisher = "Elsevier Ltd.",
journal = "Applied Clay Science",
title = "Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite",
pages = "107289",
volume = "250",
doi = "10.1016/j.clay.2024.107289"
}

Kosić, V., Božić, N., Dojnov, B., Banković, P., Jović-Jovičić, N., Knežević-Jugović, Z.,& Milutinović-Nikolić, A.. (2024-03). Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. in Applied Clay Science
Elsevier Ltd.., 250, 107289.
https://doi.org/10.1016/j.clay.2024.107289

Kosić V, Božić N, Dojnov B, Banković P, Jović-Jovičić N, Knežević-Jugović Z, Milutinović-Nikolić A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. in Applied Clay Science. 2024;250:107289.
doi:10.1016/j.clay.2024.107289 .

Kosić, Višnja, Božić, Nataša, Dojnov, Biljana, Banković, Predrag, Jović-Jovičić, Nataša, Knežević-Jugović, Zorica, Milutinović-Nikolić, Aleksandra, "Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite" in Applied Clay Science, 250 (2024-03):107289,
https://doi.org/10.1016/j.clay.2024.107289 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gökhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana B.
AU  - Milinčić, Danijel D.
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7457
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
SP  - 518
VL  - 17
DO  - 10.3390/ph17040518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gökhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana B. and Milinčić, Danijel D. and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
pages = "518",
volume = "17",
doi = "10.3390/ph17040518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M. B., Milinčić, D. D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić MB, Milinčić DD, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gökhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana B., Milinčić, Danijel D., Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Bugarski, Branko
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7288
AB  - In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is a lack of even single-phase EMFD models that can be incorporated in CFD software, while simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented, though they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.
PB  - AIP Publishing
T2  - Physics of Fluids
T1  - Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation
IS  - 2
SP  - 022019
VL  - 36
DO  - 10.1063/5.0190651
ER  - 

@article{
author = "Bošković, Stefan A. and Bugarski, Branko",
year = "2024",
abstract = "In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is a lack of even single-phase EMFD models that can be incorporated in CFD software, while simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented, though they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.",
publisher = "AIP Publishing",
journal = "Physics of Fluids",
title = "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation",
number = "2",
pages = "022019",
volume = "36",
doi = "10.1063/5.0190651"
}

Bošković, S. A.,& Bugarski, B.. (2024). Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids
AIP Publishing., 36(2), 022019.
https://doi.org/10.1063/5.0190651

Bošković SA, Bugarski B. Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids. 2024;36(2):022019.
doi:10.1063/5.0190651 .

Bošković, Stefan A., Bugarski, Branko, "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation" in Physics of Fluids, 36, no. 2 (2024):022019,
https://doi.org/10.1063/5.0190651 . .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Bugarski, Branko
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7348
AB  - In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is lack of even single-phase EMFD models that can be incorporated into CFD software; however, simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented although they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable, and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.
PB  - American Institute of Physics Inc.
T2  - Physics of Fluids
T1  - Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation
IS  - 2
SP  - 022019
VL  - 36
DO  - 10.1063/5.0190651
ER  - 

@article{
author = "Bošković, Stefan A. and Bugarski, Branko",
year = "2024",
abstract = "In this work, we present a two-phase electro-magneto-fluid dynamics (EMFD) model that merges electromagnetics and fluid dynamics. The model is suitable for use in computational fluid dynamics (CFD) software and was incorporated into OpenFOAM® after deriving appropriate equations that bypassed certain limitations of the software. Currently, there is lack of even single-phase EMFD models that can be incorporated into CFD software; however, simpler models from electrohydrodynamics (EHD) and magnetohydrodynamics (MHD) are being implemented although they have certain approximations that can limit their applicability. We conclude that the derived EMFD model is applicable, and show its quality by implementing it and analyzing the results. We use cases with a droplet and the electrospinning process for verification. The drop deformations obtained were closer to analytical predictions than in the literature for two EHD models, but some oscillations were observed. We compared one simulation to the prediction of an analytical equation from MHD, and good agreement was shown. Finally, we simulate the electrospinning process, and the results were very close to the analytical predictions. We conclude that the implementation can be used for both EHD and MHD cases.",
publisher = "American Institute of Physics Inc.",
journal = "Physics of Fluids",
title = "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation",
number = "2",
pages = "022019",
volume = "36",
doi = "10.1063/5.0190651"
}

Bošković, S. A.,& Bugarski, B.. (2024). Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids
American Institute of Physics Inc.., 36(2), 022019.
https://doi.org/10.1063/5.0190651

Bošković SA, Bugarski B. Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation. in Physics of Fluids. 2024;36(2):022019.
doi:10.1063/5.0190651 .

Bošković, Stefan A., Bugarski, Branko, "Two-phase electro-magneto-fluid dynamics model and its computational fluid dynamics implementation" in Physics of Fluids, 36, no. 2 (2024):022019,
https://doi.org/10.1063/5.0190651 . .

TY  - JOUR
AU  - Belošević, Spasoje D.
AU  - Milinčić, Danijel D.
AU  - Gašić, Uroš M.
AU  - Kostić, Aleksandar Ž.
AU  - Salević-Jelić, Ana S.
AU  - Marković, Jovana M.
AU  - Đorđević, Verica B.
AU  - Lević, Steva M.
AU  - Pešić, Mirjana B.
AU  - Nedović, Viktor A.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7383
AB  - The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C-glycosides were most abundant in red beet microgreens. Cold-pressing of microgreens into juice significantly altered the profiles of bioactive compounds. Various isothiocyanates were detected in BCJ, while more phenolic acid aglycones and their derivatives with organic acids (quinic acid and malic acid) were identified in all juices. Microgreen juices exhibited good antioxidant properties, especially ABTS•+ scavenging activity and ferric reducing antioxidant power. Microgreen juices had mild acidity, low sugar content, and good sensory acceptability and quality with the typical flavors of the respective microgreen species. Cold-pressed microgreen juices from AMJ, RBJ, and BCJ represent a rich source of bioactive compounds and can be characterized as novel functional products.
PB  - MDPI
T2  - Foods
T1  - Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties
IS  - 5
SP  - 757
VL  - 13
DO  - 10.3390/foods13050757
ER  - 

@article{
author = "Belošević, Spasoje D. and Milinčić, Danijel D. and Gašić, Uroš M. and Kostić, Aleksandar Ž. and Salević-Jelić, Ana S. and Marković, Jovana M. and Đorđević, Verica B. and Lević, Steva M. and Pešić, Mirjana B. and Nedović, Viktor A.",
year = "2024",
abstract = "The aim of this study was to analyze in detail the phytochemical composition of amaranth (AMJ), red beet (RBJ), and broccoli (BCJ) microgreens and cold-pressed juices and to evaluate the antioxidant and sensory properties of the juices. The results showed the presence of various phenolic compounds in all samples, namely betalains in amaranth and red beet microgreens, while glucosinolates were only detected in broccoli microgreens. Phenolic acids and derivatives dominated in amaranth and broccoli microgreens, while apigenin C-glycosides were most abundant in red beet microgreens. Cold-pressing of microgreens into juice significantly altered the profiles of bioactive compounds. Various isothiocyanates were detected in BCJ, while more phenolic acid aglycones and their derivatives with organic acids (quinic acid and malic acid) were identified in all juices. Microgreen juices exhibited good antioxidant properties, especially ABTS•+ scavenging activity and ferric reducing antioxidant power. Microgreen juices had mild acidity, low sugar content, and good sensory acceptability and quality with the typical flavors of the respective microgreen species. Cold-pressed microgreen juices from AMJ, RBJ, and BCJ represent a rich source of bioactive compounds and can be characterized as novel functional products.",
publisher = "MDPI",
journal = "Foods",
title = "Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties",
number = "5",
pages = "757",
volume = "13",
doi = "10.3390/foods13050757"
}

Belošević, S. D., Milinčić, D. D., Gašić, U. M., Kostić, A. Ž., Salević-Jelić, A. S., Marković, J. M., Đorđević, V. B., Lević, S. M., Pešić, M. B.,& Nedović, V. A.. (2024). Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties. in Foods
MDPI., 13(5), 757.
https://doi.org/10.3390/foods13050757

Belošević SD, Milinčić DD, Gašić UM, Kostić AŽ, Salević-Jelić AS, Marković JM, Đorđević VB, Lević SM, Pešić MB, Nedović VA. Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties. in Foods. 2024;13(5):757.
doi:10.3390/foods13050757 .

Belošević, Spasoje D., Milinčić, Danijel D., Gašić, Uroš M., Kostić, Aleksandar Ž., Salević-Jelić, Ana S., Marković, Jovana M., Đorđević, Verica B., Lević, Steva M., Pešić, Mirjana B., Nedović, Viktor A., "Broccoli, Amaranth, and Red Beet Microgreen Juices: The Influence of Cold-Pressing on the Phytochemical Composition and the Antioxidant and Sensory Properties" in Foods, 13, no. 5 (2024):757,
https://doi.org/10.3390/foods13050757 . .

TY  - DATA
AU  - Perović, Milica N.
AU  - Knežević Jugović, Zorica D.
AU  - Antov, Mirjana G.
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7331
AB  - Nanoparticles prepared by heat treatment of protein from pumpkin leaves were evaluated as potential carriers of β-carotene. White protein fraction was recovered from green juice produced by pressing the leaves, with the step of enzyme-assisted extraction (green protocol) or without it (conventional protocol). Heat treatment of white protein fractions from conventional and green protocols at 90 °C and pH 9.3 during 20 min induced formation of nanoparticles with peak diameter 18 nm and 21 nm, respectively. Due to heating, portion of β-sheets in nanoparticles from both native protein fractions decreased by approximately 15 %, associated with an increase in surface hydrophobicity-to-area ratio. Quenching constant of β-carotene for nanoparticles was increased nearly 100 times by heating the white protein fraction recovered in green protocol. Native white protein fraction from conventional protocol and corresponding nanoparticles exhibited high ability to bind β-carotene, with quenching constant 3 × 105 L/mol and 3.3 × 105 L/mol, respectively. White protein fraction from pumpkin leaves appeared to be a suitable substrate for the fabrication of nanoparticles by heat treatment, with potential application as β-carotene nanocarriers in food matrices.
PB  - Elsevier B.V.
T2  - Future Foods
T1  - Supplementary material for the article: Perović, M. N.; Knežević Jugović, Z. D.; Antov, M. G. Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. Future Foods 2024, 9, 100310. https://doi.org/10.1016/j.fufo.2024.100310
SP  - 100310
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7331
ER  - 

@misc{
author = "Perović, Milica N. and Knežević Jugović, Zorica D. and Antov, Mirjana G.",
year = "2024",
abstract = "Nanoparticles prepared by heat treatment of protein from pumpkin leaves were evaluated as potential carriers of β-carotene. White protein fraction was recovered from green juice produced by pressing the leaves, with the step of enzyme-assisted extraction (green protocol) or without it (conventional protocol). Heat treatment of white protein fractions from conventional and green protocols at 90 °C and pH 9.3 during 20 min induced formation of nanoparticles with peak diameter 18 nm and 21 nm, respectively. Due to heating, portion of β-sheets in nanoparticles from both native protein fractions decreased by approximately 15 %, associated with an increase in surface hydrophobicity-to-area ratio. Quenching constant of β-carotene for nanoparticles was increased nearly 100 times by heating the white protein fraction recovered in green protocol. Native white protein fraction from conventional protocol and corresponding nanoparticles exhibited high ability to bind β-carotene, with quenching constant 3 × 105 L/mol and 3.3 × 105 L/mol, respectively. White protein fraction from pumpkin leaves appeared to be a suitable substrate for the fabrication of nanoparticles by heat treatment, with potential application as β-carotene nanocarriers in food matrices.",
publisher = "Elsevier B.V.",
journal = "Future Foods",
title = "Supplementary material for the article: Perović, M. N.; Knežević Jugović, Z. D.; Antov, M. G. Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. Future Foods 2024, 9, 100310. https://doi.org/10.1016/j.fufo.2024.100310",
pages = "100310",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7331"
}

Perović, M. N., Knežević Jugović, Z. D.,& Antov, M. G.. (2024). Supplementary material for the article: Perović, M. N.; Knežević Jugović, Z. D.; Antov, M. G. Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. Future Foods 2024, 9, 100310. https://doi.org/10.1016/j.fufo.2024.100310. in Future Foods
Elsevier B.V.., 9, 100310.
https://hdl.handle.net/21.15107/rcub_technorep_7331

Perović MN, Knežević Jugović ZD, Antov MG. Supplementary material for the article: Perović, M. N.; Knežević Jugović, Z. D.; Antov, M. G. Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. Future Foods 2024, 9, 100310. https://doi.org/10.1016/j.fufo.2024.100310. in Future Foods. 2024;9:100310.
https://hdl.handle.net/21.15107/rcub_technorep_7331 .

Perović, Milica N., Knežević Jugović, Zorica D., Antov, Mirjana G., "Supplementary material for the article: Perović, M. N.; Knežević Jugović, Z. D.; Antov, M. G. Heat-induced nanoparticles from pumpkin leaf protein for potential application as β-carotene carriers. Future Foods 2024, 9, 100310. https://doi.org/10.1016/j.fufo.2024.100310" in Future Foods, 9 (2024):100310,
https://hdl.handle.net/21.15107/rcub_technorep_7331 .

TY  - DATA
AU  - Kosić, Višnja
AU  - Božić, Nataša
AU  - Dojnov, Biljana
AU  - Banković, Predrag
AU  - Jović-Jovičić, Nataša
AU  - Knežević-Jugović, Zorica
AU  - Milutinović-Nikolić, Aleksandra
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7330
AB  - Three glycoside hydrolases (α-amylase, xylanase and pullulanase) were immobilized on low-cost, environmentally friendly, easily modified clay rich in beidellite. Modifications included common procedures: Na-exchange, acid activation, pillaring, pillaring followed by acid activation, and organo-modifications with chitosan. Supports were characterized by chemical analysis, low temperature N2 physisorption, X-ray powder diffraction (XRPD) and Fourier-transform infrared (FT-IR) spectroscopy. The point of zero charge was also determined. Specific activity of different immobilizates of selected glycoside hydrolases was notably influenced by the type of chemical modification of supports. For each enzyme optimal support was chosen and storage stability was tested. α-Amylase immobilized on acid-activated support retained up to 95% of its initial specific activity of 105.6 ± 5.1 U g−1 after a testing period of 120 days. The most suitable support for xylanase was chitosan-modified beidellite with having specific activity of 90.0 ± 1.4 U g−1 which retained >50% its value after 120 days. Specific activity of pullulanase immobilized on pillared sample that was subsequently activated by acid was 44.5 ± 0.7 U g−1. Initial activity was preserved up to 33% for the same testing period. Comparing these results to the storage stability of the free enzymes that completely lost their activity for the longest period of 40 days, it can be concluded that appropriately modified beidellite- based clays could be used as suitable supports for stabilization of glycoside hydrolases. Nevertheless, further characterization of immobilizates (pH, thermal and operational stability) is needed in order to raise the suitability for larger scale processes in food industry.
PB  - Elsevier Ltd.
T2  - Applied Clay Science
T1  - Supplementary material for the article: Kosić, V.; Božić, B.; Dojnov, B.; Banković, P.; Jović-Jovičić, N.; Knežević-Jugović, Z.; Milutinović-Nikolić, A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. Applied Clay Science 2024, 250, 107289. https://doi.org/10.1016/j.clay.2024.107289
SP  - 107289
VL  - 250
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7330
ER  - 

@misc{
author = "Kosić, Višnja and Božić, Nataša and Dojnov, Biljana and Banković, Predrag and Jović-Jovičić, Nataša and Knežević-Jugović, Zorica and Milutinović-Nikolić, Aleksandra",
year = "2024",
abstract = "Three glycoside hydrolases (α-amylase, xylanase and pullulanase) were immobilized on low-cost, environmentally friendly, easily modified clay rich in beidellite. Modifications included common procedures: Na-exchange, acid activation, pillaring, pillaring followed by acid activation, and organo-modifications with chitosan. Supports were characterized by chemical analysis, low temperature N2 physisorption, X-ray powder diffraction (XRPD) and Fourier-transform infrared (FT-IR) spectroscopy. The point of zero charge was also determined. Specific activity of different immobilizates of selected glycoside hydrolases was notably influenced by the type of chemical modification of supports. For each enzyme optimal support was chosen and storage stability was tested. α-Amylase immobilized on acid-activated support retained up to 95% of its initial specific activity of 105.6 ± 5.1 U g−1 after a testing period of 120 days. The most suitable support for xylanase was chitosan-modified beidellite with having specific activity of 90.0 ± 1.4 U g−1 which retained >50% its value after 120 days. Specific activity of pullulanase immobilized on pillared sample that was subsequently activated by acid was 44.5 ± 0.7 U g−1. Initial activity was preserved up to 33% for the same testing period. Comparing these results to the storage stability of the free enzymes that completely lost their activity for the longest period of 40 days, it can be concluded that appropriately modified beidellite- based clays could be used as suitable supports for stabilization of glycoside hydrolases. Nevertheless, further characterization of immobilizates (pH, thermal and operational stability) is needed in order to raise the suitability for larger scale processes in food industry.",
publisher = "Elsevier Ltd.",
journal = "Applied Clay Science",
title = "Supplementary material for the article: Kosić, V.; Božić, B.; Dojnov, B.; Banković, P.; Jović-Jovičić, N.; Knežević-Jugović, Z.; Milutinović-Nikolić, A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. Applied Clay Science 2024, 250, 107289. https://doi.org/10.1016/j.clay.2024.107289",
pages = "107289",
volume = "250",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7330"
}

Kosić, V., Božić, N., Dojnov, B., Banković, P., Jović-Jovičić, N., Knežević-Jugović, Z.,& Milutinović-Nikolić, A.. (2024). Supplementary material for the article: Kosić, V.; Božić, B.; Dojnov, B.; Banković, P.; Jović-Jovičić, N.; Knežević-Jugović, Z.; Milutinović-Nikolić, A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. Applied Clay Science 2024, 250, 107289. https://doi.org/10.1016/j.clay.2024.107289. in Applied Clay Science
Elsevier Ltd.., 250, 107289.
https://hdl.handle.net/21.15107/rcub_technorep_7330

Kosić V, Božić N, Dojnov B, Banković P, Jović-Jovičić N, Knežević-Jugović Z, Milutinović-Nikolić A. Supplementary material for the article: Kosić, V.; Božić, B.; Dojnov, B.; Banković, P.; Jović-Jovičić, N.; Knežević-Jugović, Z.; Milutinović-Nikolić, A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. Applied Clay Science 2024, 250, 107289. https://doi.org/10.1016/j.clay.2024.107289. in Applied Clay Science. 2024;250:107289.
https://hdl.handle.net/21.15107/rcub_technorep_7330 .

Kosić, Višnja, Božić, Nataša, Dojnov, Biljana, Banković, Predrag, Jović-Jovičić, Nataša, Knežević-Jugović, Zorica, Milutinović-Nikolić, Aleksandra, "Supplementary material for the article: Kosić, V.; Božić, B.; Dojnov, B.; Banković, P.; Jović-Jovičić, N.; Knežević-Jugović, Z.; Milutinović-Nikolić, A. Significantly improved stabilization of glycoside hydrolases important in food industry by immobilization onto appropriately modified beidellite. Applied Clay Science 2024, 250, 107289. https://doi.org/10.1016/j.clay.2024.107289" in Applied Clay Science, 250 (2024):107289,
https://hdl.handle.net/21.15107/rcub_technorep_7330 .

Knežević Jugović, Z., Đorđević, V., Antov, M., Šekuljica, N., Mijalković, J., Jakovetić Tanasković, S., Balanč, B., Petrović, P., Salević-Jelić, A., Rakić, V., Nedović, V.,& Bugarski, B.. (2023). Колачићи на бази протеина нове генерације – протеина из лишћа уљарица. in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа
Београд : Центар за промоцију науке., 37.
https://hdl.handle.net/21.15107/rcub_technorep_7078

Knežević Jugović Z, Đorđević V, Antov M, Šekuljica N, Mijalković J, Jakovetić Tanasković S, Balanč B, Petrović P, Salević-Jelić A, Rakić V, Nedović V, Bugarski B. Колачићи на бази протеина нове генерације – протеина из лишћа уљарица. in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа. 2023;:37.
https://hdl.handle.net/21.15107/rcub_technorep_7078 .

Knežević Jugović, Zorica, Đorđević, Verica, Antov, Mirjana, Šekuljica, Nataša, Mijalković, Jelena, Jakovetić Tanasković, Sonja, Balanč, Bojana, Petrović, Predrag, Salević-Jelić, Ana, Rakić, Vesna, Nedović, Viktor, Bugarski, Branko, "Колачићи на бази протеина нове генерације – протеина из лишћа уљарица" in ИГРАЈ за човечанство! : наука за свe : каталог изложбене поставке 65. Међународни сајам технике и техничких достигнућа (2023):37,
https://hdl.handle.net/21.15107/rcub_technorep_7078 .

TY  - CONF
AU  - Mijalković, Jelena R.
AU  - Šekuljica, Nataša Ž.
AU  - Jakovetić Tanasković, Sonja M.
AU  - Petrović, Predrag M.
AU  - Balanč, Bojana D.
AU  - Đorđević, Verica B.
AU  - Knežević-Jugović, Zorica D.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6914
AB  - The potential of food biodiversity and its production streams to generate additional features for isolating high-value products has been underutilized. Even so, the recovery of valuable nutritional compounds like proteins from waste streams and by-products has been identified as a key strategy for enhancing production sustainability in order to open up new market potential. In accordance with those facts, the primary aim of this research was to utilize a relatively novel extraction technique, high-intensity ultrasound, in order to achieve a reduction in time, energy, and extraction solvent consumption while at the same time improving the extraction yield and nutritional value of extracted leaf proteins. For this purpose, an ultrasound probe system with a low frequency (20±0.2 kHz) was utilized to extract the white proteins from the pumpkin leaf biomass, and the effects of different sonication amplitudes (20, 30, 40, 50, 60 and 70%) and duration periods (0, 1, 3, 5, 7.5, 10, 12.5, 15, 17.5, and 20 min) on the yield, solubility and emulsifying qualities, antioxidant properties, and structural characteristics of proteins were studied. In the functional properties evaluated, leaf proteins isolated using ultrasound outperformed those extracted using the conventional extraction method, maceration. High-intensity ultrasound resulted in a slight but gradual decline in solubility with an increase in amplitude, but a significant increase in solubility in an acidic environment was observed with the decrease of cavitation periods. Ultrasound-extracted proteins exhibit nearly 12, 1.5, and 3-fold greater solubility compared to the maceration-extracted sample at pH 3, 4, and 5, respectively. The emulsifying activity diminishes with increasing sonication amplitude and duration but increases in emulsifying stability as sonication periods are extended. The ultrasound extraction provided pumpkin proteins with high radical scavenging activities (i.e., good electron donors) and chelating activity, with half maximal inhibitory concentrations (IC50) in the range of 0.9 to 1.5 mg/ml, and 0.3 to 0.6, respectively, especially at 20 and 40% amplitude. Raman spectroscopy, surface charge, surface hydrophobicity, and sulfhydryl group contents were employed to characterize the structural changes brought on by ultrasound cavitation, and the achieved changes were more influenced by the treatment periods and amplitudes  applied. The experimental findings show that the use of ultrasound-emerging technology for protein extraction can significantly increase the yield of pumpkin leaf protein by up to 70%. The utilization of leaf proteins in food products and dietary supplements can be augmented by combining the ultrasound periods and amplitudes to create high-value samples with better capabilities.
PB  - Zvornik : Faculty of Technology
C3  - Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023
T1  - Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities
SP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6914
ER  - 

@conference{
author = "Mijalković, Jelena R. and Šekuljica, Nataša Ž. and Jakovetić Tanasković, Sonja M. and Petrović, Predrag M. and Balanč, Bojana D. and Đorđević, Verica B. and Knežević-Jugović, Zorica D.",
year = "2023",
abstract = "The potential of food biodiversity and its production streams to generate additional features for isolating high-value products has been underutilized. Even so, the recovery of valuable nutritional compounds like proteins from waste streams and by-products has been identified as a key strategy for enhancing production sustainability in order to open up new market potential. In accordance with those facts, the primary aim of this research was to utilize a relatively novel extraction technique, high-intensity ultrasound, in order to achieve a reduction in time, energy, and extraction solvent consumption while at the same time improving the extraction yield and nutritional value of extracted leaf proteins. For this purpose, an ultrasound probe system with a low frequency (20±0.2 kHz) was utilized to extract the white proteins from the pumpkin leaf biomass, and the effects of different sonication amplitudes (20, 30, 40, 50, 60 and 70%) and duration periods (0, 1, 3, 5, 7.5, 10, 12.5, 15, 17.5, and 20 min) on the yield, solubility and emulsifying qualities, antioxidant properties, and structural characteristics of proteins were studied. In the functional properties evaluated, leaf proteins isolated using ultrasound outperformed those extracted using the conventional extraction method, maceration. High-intensity ultrasound resulted in a slight but gradual decline in solubility with an increase in amplitude, but a significant increase in solubility in an acidic environment was observed with the decrease of cavitation periods. Ultrasound-extracted proteins exhibit nearly 12, 1.5, and 3-fold greater solubility compared to the maceration-extracted sample at pH 3, 4, and 5, respectively. The emulsifying activity diminishes with increasing sonication amplitude and duration but increases in emulsifying stability as sonication periods are extended. The ultrasound extraction provided pumpkin proteins with high radical scavenging activities (i.e., good electron donors) and chelating activity, with half maximal inhibitory concentrations (IC50) in the range of 0.9 to 1.5 mg/ml, and 0.3 to 0.6, respectively, especially at 20 and 40% amplitude. Raman spectroscopy, surface charge, surface hydrophobicity, and sulfhydryl group contents were employed to characterize the structural changes brought on by ultrasound cavitation, and the achieved changes were more influenced by the treatment periods and amplitudes  applied. The experimental findings show that the use of ultrasound-emerging technology for protein extraction can significantly increase the yield of pumpkin leaf protein by up to 70%. The utilization of leaf proteins in food products and dietary supplements can be augmented by combining the ultrasound periods and amplitudes to create high-value samples with better capabilities.",
publisher = "Zvornik : Faculty of Technology",
journal = "Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023",
title = "Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities",
pages = "178",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6914"
}

Mijalković, J. R., Šekuljica, N. Ž., Jakovetić Tanasković, S. M., Petrović, P. M., Balanč, B. D., Đorđević, V. B.,& Knežević-Jugović, Z. D.. (2023). Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities. in Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023
Zvornik : Faculty of Technology., 178.
https://hdl.handle.net/21.15107/rcub_technorep_6914

Mijalković JR, Šekuljica NŽ, Jakovetić Tanasković SM, Petrović PM, Balanč BD, Đorđević VB, Knežević-Jugović ZD. Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities. in Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023. 2023;:178.
https://hdl.handle.net/21.15107/rcub_technorep_6914 .

Mijalković, Jelena R., Šekuljica, Nataša Ž., Jakovetić Tanasković, Sonja M., Petrović, Predrag M., Balanč, Bojana D., Đorđević, Verica B., Knežević-Jugović, Zorica D., "Ultrasound-emerging technology for valorization of pumpkin leaf biomass: impact of sonication parameters on protein recovery, structure, functionalities, and bioactivities" in Book of Abstracts / VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, March 20-23, 2023 (2023):178,
https://hdl.handle.net/21.15107/rcub_technorep_6914 .

TY  - CONF
AU  - Jakovetić Tanasković, Sonja
AU  - Šekuljica, Nataša
AU  - Mijalković, Jelena
AU  - Gazikalović, Ivana
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7289
AB  - The projection that the world's population will reach nearly 10 billion people
by 2050 serve as a clear signal for the urgent need to find sustainable solutions that can
provide enough food for the growing population, along with reducing environmental
impact. To achieve this, a global transition towards sustainable nutrition that fulfills all
nutritional requirements is essential. Currently, the primary sources of protein, essential
macronutrients in our diet, are of animal origin. This presents a significant challenge for
the environment, given that the livestock system is responsible for emitting significant
amount of greenhouse gases (GHG). As a result, finding alternative protein sources
becomes imperative goal of circular bioeconomy in order to meet the demands for this
crucial macronutrient while respecting the principles of sustainable development. Leafy
biomass presents a good alternative protein source with respect to both nutritive and
sustainability demands.
AB  - Prognoza da će do 2050. godine na planeti biti gotovo 10 milijardi ljudi
predstavlja signal za hitno pronalaženje održivih rešenja koja bi omogućila dovoljno
hrane za rastuću populaciju uz smanjenje ekološkog otiska. Da bi se ovo postiglo
neophodna je globalna tranzicija prema održivoj ishrani koja pritom ispunjava sve
nutritivne zahteve. Osnovni izvori proteina, esencijalnih makronutrijenata, u ishrani su
životinjskog porekla, što predstavlja ozbiljan problem za životnu sredinu, jer je samo
stočarska industrija odgovorna za značajnu emisiju gasova staklene baste. Pronalaženje alternativnih izvora proteina tako postaje imperativ cirkularne bioekonomije kako bi se
zadovoljile potrebe za ovim makronutrijenom uz istovremeno poštovanje principa
održivog razvoja. Lisna biomasa, predstavlja dobar alternativni izvor proteina i sa
nutritivnog i održivog aspekta.
PB  - Beograd : Fakultet organizacionih nauka
C3  - Digitalni i zeleni razvoj : zbornik radova / XIV Skup privrednika i naučnika SPIN’23, 6. - 7. novembar 2023., Beograd, Srbija
T1  - LEAF AS AN ALTERNATIVE PROTEIN SOURCE - TOWARD CIRCULAR BIOECONOMY
T1  - LIST KAO ALTERNATIVNI IZVOR PROTEINA - KA CIRKULARNOJ BIOEKONOMIJI
EP  - 117
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7289
ER  - 

@conference{
author = "Jakovetić Tanasković, Sonja and Šekuljica, Nataša and Mijalković, Jelena and Gazikalović, Ivana and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2023",
abstract = "The projection that the world's population will reach nearly 10 billion people
by 2050 serve as a clear signal for the urgent need to find sustainable solutions that can
provide enough food for the growing population, along with reducing environmental
impact. To achieve this, a global transition towards sustainable nutrition that fulfills all
nutritional requirements is essential. Currently, the primary sources of protein, essential
macronutrients in our diet, are of animal origin. This presents a significant challenge for
the environment, given that the livestock system is responsible for emitting significant
amount of greenhouse gases (GHG). As a result, finding alternative protein sources
becomes imperative goal of circular bioeconomy in order to meet the demands for this
crucial macronutrient while respecting the principles of sustainable development. Leafy
biomass presents a good alternative protein source with respect to both nutritive and
sustainability demands., Prognoza da će do 2050. godine na planeti biti gotovo 10 milijardi ljudi
predstavlja signal za hitno pronalaženje održivih rešenja koja bi omogućila dovoljno
hrane za rastuću populaciju uz smanjenje ekološkog otiska. Da bi se ovo postiglo
neophodna je globalna tranzicija prema održivoj ishrani koja pritom ispunjava sve
nutritivne zahteve. Osnovni izvori proteina, esencijalnih makronutrijenata, u ishrani su
životinjskog porekla, što predstavlja ozbiljan problem za životnu sredinu, jer je samo
stočarska industrija odgovorna za značajnu emisiju gasova staklene baste. Pronalaženje alternativnih izvora proteina tako postaje imperativ cirkularne bioekonomije kako bi se
zadovoljile potrebe za ovim makronutrijenom uz istovremeno poštovanje principa
održivog razvoja. Lisna biomasa, predstavlja dobar alternativni izvor proteina i sa
nutritivnog i održivog aspekta.",
publisher = "Beograd : Fakultet organizacionih nauka",
journal = "Digitalni i zeleni razvoj : zbornik radova / XIV Skup privrednika i naučnika SPIN’23, 6. - 7. novembar 2023., Beograd, Srbija",
title = "LEAF AS AN ALTERNATIVE PROTEIN SOURCE - TOWARD CIRCULAR BIOECONOMY, LIST KAO ALTERNATIVNI IZVOR PROTEINA - KA CIRKULARNOJ BIOEKONOMIJI",
pages = "117-109",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7289"
}

Jakovetić Tanasković, S., Šekuljica, N., Mijalković, J., Gazikalović, I., Luković, N.,& Knežević-Jugović, Z.. (2023). LEAF AS AN ALTERNATIVE PROTEIN SOURCE - TOWARD CIRCULAR BIOECONOMY. in Digitalni i zeleni razvoj : zbornik radova / XIV Skup privrednika i naučnika SPIN’23, 6. - 7. novembar 2023., Beograd, Srbija
Beograd : Fakultet organizacionih nauka., 109-117.
https://hdl.handle.net/21.15107/rcub_technorep_7289

Jakovetić Tanasković S, Šekuljica N, Mijalković J, Gazikalović I, Luković N, Knežević-Jugović Z. LEAF AS AN ALTERNATIVE PROTEIN SOURCE - TOWARD CIRCULAR BIOECONOMY. in Digitalni i zeleni razvoj : zbornik radova / XIV Skup privrednika i naučnika SPIN’23, 6. - 7. novembar 2023., Beograd, Srbija. 2023;:109-117.
https://hdl.handle.net/21.15107/rcub_technorep_7289 .

Jakovetić Tanasković, Sonja, Šekuljica, Nataša, Mijalković, Jelena, Gazikalović, Ivana, Luković, Nevena, Knežević-Jugović, Zorica, "LEAF AS AN ALTERNATIVE PROTEIN SOURCE - TOWARD CIRCULAR BIOECONOMY" in Digitalni i zeleni razvoj : zbornik radova / XIV Skup privrednika i naučnika SPIN’23, 6. - 7. novembar 2023., Beograd, Srbija (2023):109-117,
https://hdl.handle.net/21.15107/rcub_technorep_7289 .

TY  - CONF
AU  - Mijalković, Jelena
AU  - Šekuljica, Nataša
AU  - Jakovetić Tanasković, Sonja
AU  - Luković, Nevena
AU  - Pavlović, Neda
AU  - Bakrač, Jelena
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6913
AB  - RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) is a non-allergenic, easily digestible protein found in the leaves of C3 plants, accounting for up to 50% of the total. It is similar to the FAO's ideal protein and is offered as a valuable alternative for meeting nutritional requirements. Plant-based proteins provide amino acids for human cells development and act as precursors of bioactive peptides. Protein sources and proteolytic enzymes are crucial for producing bioactive peptides effectively.
This research aimed to optimize the hydrolysis of pumpkin leaf-isolated protein in terms of time and type of process (one or two-step) using endo- and exo-peptidases. Efficiency was assessed using SDS-PAGE electrophoresis, quantitative hydrolysis analysis, and peptides' capacity to chelate Fe2+ ions and scavenge ABTS•+ radical cations. The peptide molecular weight was determined by implementing the size-exclusion UFLC method.
The highest degree of hydrolysis, which is correlated with higher antioxidant activity, was shown by Alcalase and Everlase (19.5%), and Neutrase (21.5%) with a tendency to favor Neutrase due to more favorable process conditions and a more sensory-acceptable product. Hydrolysis with Neutrase-Flavourzyme during 225 min yielded hydrolyzates with a 43.5% degree of hydrolysis, and antioxidant activities of 0.74 μmol TEAA/mg (i.e. 48%) and 0.30 μmol EEAA/mg (i.e. 44%). Five peptide fractions were identified as follows: F1 (> 27 kDa), F2 (20-27 kDa), F3 (10-20 kDa), F4 (3-10 kDa), and F5 (< 3 kDa). By establishing a correlation with antioxidant activity, it has been proven that a large proportion of peptide fractions F3, F4, and F5 were accountable for Neutrase-Flavourzyme hydrolyzate's good antioxidant activity. Examined enzymatic approches contributed to generate the antioxidant peptides from pumpkin-leaf proteins with diverse peptide profiles.
PB  - Skopje : Society of chemists and technologists of Macedonia
C3  - Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia
T1  - Pumpkin leaf-isolated RuBisCO as a protein source for bioactive peptides
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6913
ER  - 

@conference{
author = "Mijalković, Jelena and Šekuljica, Nataša and Jakovetić Tanasković, Sonja and Luković, Nevena and Pavlović, Neda and Bakrač, Jelena and Knežević-Jugović, Zorica",
year = "2023",
abstract = "RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) is a non-allergenic, easily digestible protein found in the leaves of C3 plants, accounting for up to 50% of the total. It is similar to the FAO's ideal protein and is offered as a valuable alternative for meeting nutritional requirements. Plant-based proteins provide amino acids for human cells development and act as precursors of bioactive peptides. Protein sources and proteolytic enzymes are crucial for producing bioactive peptides effectively.
This research aimed to optimize the hydrolysis of pumpkin leaf-isolated protein in terms of time and type of process (one or two-step) using endo- and exo-peptidases. Efficiency was assessed using SDS-PAGE electrophoresis, quantitative hydrolysis analysis, and peptides' capacity to chelate Fe2+ ions and scavenge ABTS•+ radical cations. The peptide molecular weight was determined by implementing the size-exclusion UFLC method.
The highest degree of hydrolysis, which is correlated with higher antioxidant activity, was shown by Alcalase and Everlase (19.5%), and Neutrase (21.5%) with a tendency to favor Neutrase due to more favorable process conditions and a more sensory-acceptable product. Hydrolysis with Neutrase-Flavourzyme during 225 min yielded hydrolyzates with a 43.5% degree of hydrolysis, and antioxidant activities of 0.74 μmol TEAA/mg (i.e. 48%) and 0.30 μmol EEAA/mg (i.e. 44%). Five peptide fractions were identified as follows: F1 (> 27 kDa), F2 (20-27 kDa), F3 (10-20 kDa), F4 (3-10 kDa), and F5 (< 3 kDa). By establishing a correlation with antioxidant activity, it has been proven that a large proportion of peptide fractions F3, F4, and F5 were accountable for Neutrase-Flavourzyme hydrolyzate's good antioxidant activity. Examined enzymatic approches contributed to generate the antioxidant peptides from pumpkin-leaf proteins with diverse peptide profiles.",
publisher = "Skopje : Society of chemists and technologists of Macedonia",
journal = "Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia",
title = "Pumpkin leaf-isolated RuBisCO as a protein source for bioactive peptides",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6913"
}

Mijalković, J., Šekuljica, N., Jakovetić Tanasković, S., Luković, N., Pavlović, N., Bakrač, J.,& Knežević-Jugović, Z.. (2023). Pumpkin leaf-isolated RuBisCO as a protein source for bioactive peptides. in Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia
Skopje : Society of chemists and technologists of Macedonia., 149.
https://hdl.handle.net/21.15107/rcub_technorep_6913

Mijalković J, Šekuljica N, Jakovetić Tanasković S, Luković N, Pavlović N, Bakrač J, Knežević-Jugović Z. Pumpkin leaf-isolated RuBisCO as a protein source for bioactive peptides. in Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia. 2023;:149.
https://hdl.handle.net/21.15107/rcub_technorep_6913 .

Mijalković, Jelena, Šekuljica, Nataša, Jakovetić Tanasković, Sonja, Luković, Nevena, Pavlović, Neda, Bakrač, Jelena, Knežević-Jugović, Zorica, "Pumpkin leaf-isolated RuBisCO as a protein source for bioactive peptides" in Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia (2023):149,
https://hdl.handle.net/21.15107/rcub_technorep_6913 .

TY  - CONF
AU  - Šekuljica, Nataša
AU  - Mijalković, Jelena
AU  - Jakovetić Tanasković, Sonja
AU  - Korićanac, Marija
AU  - Gazikalović, Ivana
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7020
AB  - Incorporation of alternative plant protein concentrates or isolates into cereal-based matrices can lead to the production of nutritionally improved products such as bread with high protein content and an improved essential amino acid profile, especially lysine. Green leaves, as the richest protein source containing soluble – white and insoluble – green proteins, seem to be an attractive and sustainable source for obtaining alternative proteins in line with the concept of green biorefinery, compared to the cultivation of soybeans for protein production. Thus, the overall objective of this study was to evaluate the quality of white and green proteins from various leaf sources for inclusion in a library of alternative proteins and for use in the development of plant protein-based products.
A food-grade, sustainable three-step process was used to produce the protein concentrate from the pumpkin and spinach leaves, including mechanical pressing of the leaves with screw presses, thermal coagulation, and acid precipitation. The functional properties of the white and green protein concentrates, such as solubility, emulsifying activity/stability, and water/oil holding capacity, were investigated. The rheological behaviour of wheat flour enriched with leaf protein was studied using the Mixolab Chopin. The functional properties of the leaf protein concentrates, as well as, the rheological properties of the leaf protein-enriched flour were compared with a soy protein concentrate that served as a reference.
The leaf protein concentrates showed comparable or better functional properties than the currently used soy protein concentrates. The changes in the rheological behaviour were observed in terms of water absorption, development and stability time, mechanical resistance during mixing, and behaviour of the protein-enriched flour during heating. This study has shown that leaf protein has the potential for the production of leaf protein-rich baked goods.
PB  - Skopje : Society of chemists and technologists of Macedonia
C3  - Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia
T1  - Testing The Quality of White and Green Leaf Proteins Using Mixolabtm for Applications in Bakery Products Formulations
SP  - 148
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7020
ER  - 

@conference{
author = "Šekuljica, Nataša and Mijalković, Jelena and Jakovetić Tanasković, Sonja and Korićanac, Marija and Gazikalović, Ivana and Knežević-Jugović, Zorica",
year = "2023",
abstract = "Incorporation of alternative plant protein concentrates or isolates into cereal-based matrices can lead to the production of nutritionally improved products such as bread with high protein content and an improved essential amino acid profile, especially lysine. Green leaves, as the richest protein source containing soluble – white and insoluble – green proteins, seem to be an attractive and sustainable source for obtaining alternative proteins in line with the concept of green biorefinery, compared to the cultivation of soybeans for protein production. Thus, the overall objective of this study was to evaluate the quality of white and green proteins from various leaf sources for inclusion in a library of alternative proteins and for use in the development of plant protein-based products.
A food-grade, sustainable three-step process was used to produce the protein concentrate from the pumpkin and spinach leaves, including mechanical pressing of the leaves with screw presses, thermal coagulation, and acid precipitation. The functional properties of the white and green protein concentrates, such as solubility, emulsifying activity/stability, and water/oil holding capacity, were investigated. The rheological behaviour of wheat flour enriched with leaf protein was studied using the Mixolab Chopin. The functional properties of the leaf protein concentrates, as well as, the rheological properties of the leaf protein-enriched flour were compared with a soy protein concentrate that served as a reference.
The leaf protein concentrates showed comparable or better functional properties than the currently used soy protein concentrates. The changes in the rheological behaviour were observed in terms of water absorption, development and stability time, mechanical resistance during mixing, and behaviour of the protein-enriched flour during heating. This study has shown that leaf protein has the potential for the production of leaf protein-rich baked goods.",
publisher = "Skopje : Society of chemists and technologists of Macedonia",
journal = "Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia",
title = "Testing The Quality of White and Green Leaf Proteins Using Mixolabtm for Applications in Bakery Products Formulations",
pages = "148",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7020"
}

Šekuljica, N., Mijalković, J., Jakovetić Tanasković, S., Korićanac, M., Gazikalović, I.,& Knežević-Jugović, Z.. (2023). Testing The Quality of White and Green Leaf Proteins Using Mixolabtm for Applications in Bakery Products Formulations. in Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia
Skopje : Society of chemists and technologists of Macedonia., 148.
https://hdl.handle.net/21.15107/rcub_technorep_7020

Šekuljica N, Mijalković J, Jakovetić Tanasković S, Korićanac M, Gazikalović I, Knežević-Jugović Z. Testing The Quality of White and Green Leaf Proteins Using Mixolabtm for Applications in Bakery Products Formulations. in Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia. 2023;:148.
https://hdl.handle.net/21.15107/rcub_technorep_7020 .

Šekuljica, Nataša, Mijalković, Jelena, Jakovetić Tanasković, Sonja, Korićanac, Marija, Gazikalović, Ivana, Knežević-Jugović, Zorica, "Testing The Quality of White and Green Leaf Proteins Using Mixolabtm for Applications in Bakery Products Formulations" in Book of abstracts / 26th Congress of SCTM with international participation 20–23 September 2023 Metropol Lake Resort Ohrid, R. Macedonia (2023):148,
https://hdl.handle.net/21.15107/rcub_technorep_7020 .

TY  - JOUR
AU  - Šekuljica, Nataša
AU  - Jakovetić Tanasković, Sonja
AU  - Mijalković, Jelena
AU  - Simović, Milica
AU  - Pavlović, Neda
AU  - Đorđević, Nikola
AU  - Culetu, Alina
AU  - Gazikalović, Ivana
AU  - Luković, Nevena
AU  - Bakrač, Jelena
AU  - Knežević-Jugović, Zorica
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7084
AB  - Research background. The development of a novel process for the production of xy-looligosaccharides (XOS) based on the 4R concept is made possible by the integration of numerous techniques, especially enzymatic modification together with the physical pre-treatment of renewable materials. This study aims to integrate the use of agricultural wastes for the production of xylanase by a new strain of Penicillium sp. and value-added products, XOS. Experimental approach. For the production of xylanase, a solid-state fermentation was performed using wheat bran as substrate. To obtain the most active crude extract of xy-lanase, the time frame of cultivation was first adjusted. Then, the downstream process for xylanase purification was developed by combining different membrane separation units with size exclusion chromatography. Further characterisation included determination of the optimal pH and temperature, determination of the molecular mass of the purified xy-lanase and analysis of kinetic parameters. Subsequently, the hydrolytic ability of the par-tially purified xylanase in the hydrolysis of alkali-extracted hemicellulose from soybean hulls was investigated. Results and conclusions. Our results show that Penicillium rubens produced extracellular xylanase at a yield of 21 U/g during solid-state fermentation. Using two ultrafiltration membranes of 10 and 3 kDa in combination with size exclusion chromatography, a yield of 49 % and 13-fold purification of xylanase was achieved. The purified xylanase (35 kDa) cleaved linear bonds β-(1→4) in beechwood xylan at a maximum rate of 0.64 μmol/ (min·mg) and a Michaelis constant of 44 mg/mL. At pH=6 and 45 °C, the purified xylanase showed its maximum activity. The xylanase produced showed a high ability to hydrolyse the hemicellulose fraction isolated from soybean hulls, as confirmed by thin-layer chro-matography. In the hydrothermally pretreated hemicellulose hydrolysate, the content of XOS with different degrees of polymerisation was detected, while in the non-pretreated hemicellulose hydrolysate, the content of xylotriose and glucose was confirmed. Novelty and scientific contribution. Future research focusing on the creation of new en-zymatic pathways for use in processes to convert renewable materials into value-added products can draw on our findings.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls
EP  - 450
IS  - 4
SP  - 439
VL  - 61
DO  - 10.17113/ftb.61.04.23.8073
ER  - 

@article{
author = "Šekuljica, Nataša and Jakovetić Tanasković, Sonja and Mijalković, Jelena and Simović, Milica and Pavlović, Neda and Đorđević, Nikola and Culetu, Alina and Gazikalović, Ivana and Luković, Nevena and Bakrač, Jelena and Knežević-Jugović, Zorica",
year = "2023",
abstract = "Research background. The development of a novel process for the production of xy-looligosaccharides (XOS) based on the 4R concept is made possible by the integration of numerous techniques, especially enzymatic modification together with the physical pre-treatment of renewable materials. This study aims to integrate the use of agricultural wastes for the production of xylanase by a new strain of Penicillium sp. and value-added products, XOS. Experimental approach. For the production of xylanase, a solid-state fermentation was performed using wheat bran as substrate. To obtain the most active crude extract of xy-lanase, the time frame of cultivation was first adjusted. Then, the downstream process for xylanase purification was developed by combining different membrane separation units with size exclusion chromatography. Further characterisation included determination of the optimal pH and temperature, determination of the molecular mass of the purified xy-lanase and analysis of kinetic parameters. Subsequently, the hydrolytic ability of the par-tially purified xylanase in the hydrolysis of alkali-extracted hemicellulose from soybean hulls was investigated. Results and conclusions. Our results show that Penicillium rubens produced extracellular xylanase at a yield of 21 U/g during solid-state fermentation. Using two ultrafiltration membranes of 10 and 3 kDa in combination with size exclusion chromatography, a yield of 49 % and 13-fold purification of xylanase was achieved. The purified xylanase (35 kDa) cleaved linear bonds β-(1→4) in beechwood xylan at a maximum rate of 0.64 μmol/ (min·mg) and a Michaelis constant of 44 mg/mL. At pH=6 and 45 °C, the purified xylanase showed its maximum activity. The xylanase produced showed a high ability to hydrolyse the hemicellulose fraction isolated from soybean hulls, as confirmed by thin-layer chro-matography. In the hydrothermally pretreated hemicellulose hydrolysate, the content of XOS with different degrees of polymerisation was detected, while in the non-pretreated hemicellulose hydrolysate, the content of xylotriose and glucose was confirmed. Novelty and scientific contribution. Future research focusing on the creation of new en-zymatic pathways for use in processes to convert renewable materials into value-added products can draw on our findings.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls",
pages = "450-439",
number = "4",
volume = "61",
doi = "10.17113/ftb.61.04.23.8073"
}

Šekuljica, N., Jakovetić Tanasković, S., Mijalković, J., Simović, M., Pavlović, N., Đorđević, N., Culetu, A., Gazikalović, I., Luković, N., Bakrač, J.,& Knežević-Jugović, Z.. (2023). Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls. in Food Technology and Biotechnology
University of Zagreb., 61(4), 439-450.
https://doi.org/10.17113/ftb.61.04.23.8073

Šekuljica N, Jakovetić Tanasković S, Mijalković J, Simović M, Pavlović N, Đorđević N, Culetu A, Gazikalović I, Luković N, Bakrač J, Knežević-Jugović Z. Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls. in Food Technology and Biotechnology. 2023;61(4):439-450.
doi:10.17113/ftb.61.04.23.8073 .

Šekuljica, Nataša, Jakovetić Tanasković, Sonja, Mijalković, Jelena, Simović, Milica, Pavlović, Neda, Đorđević, Nikola, Culetu, Alina, Gazikalović, Ivana, Luković, Nevena, Bakrač, Jelena, Knežević-Jugović, Zorica, "Xylanase Production by Solid-State Fermentation for the Extraction of Xylooligosaccharides from Soybean Hulls" in Food Technology and Biotechnology, 61, no. 4 (2023):439-450,
https://doi.org/10.17113/ftb.61.04.23.8073 . .

TY  - CONF
AU  - Pavlović, Neda
AU  - Mijalković, Jelena
AU  - Šekuljica, Nataša
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6251
AB  - Nowadays, the necessity for dietary nutrient encapsulation emerges largely since the action of digestive enzymes causes the loss of structural integrity and function of nutrients. Proteins have high nutritional value and thus are considered as the most nutritionally beneficial carriers; moreover, due to their versatility, proteins can be generated in a variety of structures (nanoparticles, nanofilms, nanofibers, coacervates, and hydrogels), allowing for the delivery of hydrophobic or hydrophilic nutrients. Proteins of vegetable origin have broader application potential over proteins of animal sources. Thus, the modern research focuses on waste leaf biomass, such as pumpkin leaves, as a rich source of plant proteins, specifically the most abundant protein in the world, ribulose-1,5-bisphosphate carboxylase-oxygenase, RuBisCo protein. 
The aim of this work was to isolate the water-soluble proteins, mostly RuBisCO protein, from pumpkin leaves in order to utilize it as a carrier for folic acid (FA) encapsulation. The resulting protein leaf concentrate was lyophilized and used as such to form nanoparticles. Leaf protein nanoparticles were prepared by employing a Ca-induced cold gelation method. 
Results and conclusions. The results showed that the Ca-induced protein nanoparticles have a small particle size (58–208 nm), polydispersity index (PDI) of 0.241 to 0.377, and negative zeta potential (-16.2 to -23.9 mV). Protein leaf nanoparticles exhibited uniform unimodal size distribution and spherical shape with a unique honeycomb-like core structure. Nanoparticle characteristics including size, surface charge and hydrophobicity could be adjustable by changing calcium chloride concentration from 2 to 10 mM and environmental pH (7 to 9). All manufactured nanoparticles demonstrated good stability over seven days, with no significant changes in zeta potential or formation agglomerates. Release studies showed that the leaf protein nanoparticles with FA, encapsulation efficiency amounted 40.2%, were resistant to pepsin and low pH in simulated gastric fluid (10% of initial FA amount was realized), but released the encapsulated FA in simulated intestinal conditions was enriched, even another 50% was achieved.  
Pumpkin leaf protein nanoparticles have a promising possibility for bioactive ingredient delivery owing to the characteristics described above. These findings would be of great importance for the development of food-grade nanoparticles suitable for the formulations of functional foods.
PB  - Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists
C3  - Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity
T1  - Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins
EP  - 142
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6251
ER  - 

@conference{
author = "Pavlović, Neda and Mijalković, Jelena and Šekuljica, Nataša and Petrović, Predrag and Đorđević, Verica and Bugarski, Branko and Knežević-Jugović, Zorica",
year = "2022",
abstract = "Nowadays, the necessity for dietary nutrient encapsulation emerges largely since the action of digestive enzymes causes the loss of structural integrity and function of nutrients. Proteins have high nutritional value and thus are considered as the most nutritionally beneficial carriers; moreover, due to their versatility, proteins can be generated in a variety of structures (nanoparticles, nanofilms, nanofibers, coacervates, and hydrogels), allowing for the delivery of hydrophobic or hydrophilic nutrients. Proteins of vegetable origin have broader application potential over proteins of animal sources. Thus, the modern research focuses on waste leaf biomass, such as pumpkin leaves, as a rich source of plant proteins, specifically the most abundant protein in the world, ribulose-1,5-bisphosphate carboxylase-oxygenase, RuBisCo protein. 
The aim of this work was to isolate the water-soluble proteins, mostly RuBisCO protein, from pumpkin leaves in order to utilize it as a carrier for folic acid (FA) encapsulation. The resulting protein leaf concentrate was lyophilized and used as such to form nanoparticles. Leaf protein nanoparticles were prepared by employing a Ca-induced cold gelation method. 
Results and conclusions. The results showed that the Ca-induced protein nanoparticles have a small particle size (58–208 nm), polydispersity index (PDI) of 0.241 to 0.377, and negative zeta potential (-16.2 to -23.9 mV). Protein leaf nanoparticles exhibited uniform unimodal size distribution and spherical shape with a unique honeycomb-like core structure. Nanoparticle characteristics including size, surface charge and hydrophobicity could be adjustable by changing calcium chloride concentration from 2 to 10 mM and environmental pH (7 to 9). All manufactured nanoparticles demonstrated good stability over seven days, with no significant changes in zeta potential or formation agglomerates. Release studies showed that the leaf protein nanoparticles with FA, encapsulation efficiency amounted 40.2%, were resistant to pepsin and low pH in simulated gastric fluid (10% of initial FA amount was realized), but released the encapsulated FA in simulated intestinal conditions was enriched, even another 50% was achieved.  
Pumpkin leaf protein nanoparticles have a promising possibility for bioactive ingredient delivery owing to the characteristics described above. These findings would be of great importance for the development of food-grade nanoparticles suitable for the formulations of functional foods.",
publisher = "Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists",
journal = "Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity",
title = "Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins",
pages = "142-141",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6251"
}

Pavlović, N., Mijalković, J., Šekuljica, N., Petrović, P., Đorđević, V., Bugarski, B.,& Knežević-Jugović, Z.. (2022). Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins. in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity
Zagreb, Croatia : Croatian Society of Food Technologists, Biotechnologists and Nutritionists., 141-142.
https://hdl.handle.net/21.15107/rcub_technorep_6251

Pavlović N, Mijalković J, Šekuljica N, Petrović P, Đorđević V, Bugarski B, Knežević-Jugović Z. Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins. in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity. 2022;:141-142.
https://hdl.handle.net/21.15107/rcub_technorep_6251 .

Pavlović, Neda, Mijalković, Jelena, Šekuljica, Nataša, Petrović, Predrag, Đorđević, Verica, Bugarski, Branko, Knežević-Jugović, Zorica, "Cold gelation of leaf protein concentrate for nanoencapsulation of vitamins" in Book of Abstracts / 10th International Congress of Food Technologists, Biotechnologists and Nutritionists - Smart Food for a Healthy Planet and Human Prosperity (2022):141-142,
https://hdl.handle.net/21.15107/rcub_technorep_6251 .

TY  - JOUR
AU  - Bošković, Stefan A.
AU  - Karač, Aleksandar
AU  - Vrhovac, Slobodan B.
AU  - Aleksandar, Belić
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6619
AB  - The electrohydrodynamics' (EHD) perfect dielectric model was added into computational fluid dynamics (CFD) software OpenFOAM® in order to improve its usability for the EHD field and specifically for the mentioned model. Based on the investigated literature, it can be said that this is the most complete implementation of the said model. Two sets of numerical simulations with two different fluids are presented and analyzed. One set is one-dimensional. The other set is with a drop of one fluid surrounded by other fluid. Oscillations can be observed with certain expressions or calculation strategies for the electrostrictive force, and used for disregarding them. Results that are closer to analytical predictions can be obtained by using appropriate expression for the dielectric force. The electrostrictive force was implemented not only for nonpolar, but also for polar fluids, and it is shown that it might significantly influence the drop deformation. Calculated and analytically predicted drop deformations were close or comparable even up to around 0.25, what is significantly higher and different from a previous study made by other authors. Different expressions for the electric permittivity and usage of limiters for volume fractions were investigated. Conclusions from this paper can be transferred to more complicated models.
PB  - University of East Sarajevo, Faculty of Technology Zvornik
T2  - Journal of Engineering & Processing Management
T1  - Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®
EP  - 75
IS  - 2
SP  - 66
VL  - 14
DO  - 10.7251/JEPM2202066B
ER  - 

@article{
author = "Bošković, Stefan A. and Karač, Aleksandar and Vrhovac, Slobodan B. and Aleksandar, Belić and Bugarski, Branko",
year = "2022",
abstract = "The electrohydrodynamics' (EHD) perfect dielectric model was added into computational fluid dynamics (CFD) software OpenFOAM® in order to improve its usability for the EHD field and specifically for the mentioned model. Based on the investigated literature, it can be said that this is the most complete implementation of the said model. Two sets of numerical simulations with two different fluids are presented and analyzed. One set is one-dimensional. The other set is with a drop of one fluid surrounded by other fluid. Oscillations can be observed with certain expressions or calculation strategies for the electrostrictive force, and used for disregarding them. Results that are closer to analytical predictions can be obtained by using appropriate expression for the dielectric force. The electrostrictive force was implemented not only for nonpolar, but also for polar fluids, and it is shown that it might significantly influence the drop deformation. Calculated and analytically predicted drop deformations were close or comparable even up to around 0.25, what is significantly higher and different from a previous study made by other authors. Different expressions for the electric permittivity and usage of limiters for volume fractions were investigated. Conclusions from this paper can be transferred to more complicated models.",
publisher = "University of East Sarajevo, Faculty of Technology Zvornik",
journal = "Journal of Engineering & Processing Management",
title = "Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®",
pages = "75-66",
number = "2",
volume = "14",
doi = "10.7251/JEPM2202066B"
}

Bošković, S. A., Karač, A., Vrhovac, S. B., Aleksandar, B.,& Bugarski, B.. (2022). Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology Zvornik., 14(2), 66-75.
https://doi.org/10.7251/JEPM2202066B

Bošković SA, Karač A, Vrhovac SB, Aleksandar B, Bugarski B. Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®. in Journal of Engineering & Processing Management. 2022;14(2):66-75.
doi:10.7251/JEPM2202066B .

Bošković, Stefan A., Karač, Aleksandar, Vrhovac, Slobodan B., Aleksandar, Belić, Bugarski, Branko, "Implementation of the electrohydrodynamics' perfect dielectric model in OpenFOAM®" in Journal of Engineering & Processing Management, 14, no. 2 (2022):66-75,
https://doi.org/10.7251/JEPM2202066B . .

TY  - CONF
AU  - Mijalković, Jelena
AU  - Šekuljica, Nataša
AU  - Biçer, Furkan
AU  - Pavlović, Neda
AU  - Jakovetić Tanasković, Sonja
AU  - Knežević-Jugović, Zorica
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6252
AB  - Proteins are an essential component of the human diet and effectively are provided from
both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase
(RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry,
is a promising tool for obtaining protein with an excellent nutritional profile under the
green biorafinery concept, that can be used as a substitute for animal proteins. This study
provides the results regarding the implementation of ultrasound-assisted processing (UAP)
in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was
optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and
time. UAP appears to have a strong potential for recovering RuBisCO protein from
pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP
amplitude and time.
AB  - Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema
konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat
karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije
prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom
vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom
radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća
bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem
odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka.
Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća
bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.
PB  - Beograd : Srpsko hemijsko društvo = Serbian Chemical Society
C3  - Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade
T1  - Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves
EP  - 174
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6252
ER  - 

@conference{
author = "Mijalković, Jelena and Šekuljica, Nataša and Biçer, Furkan and Pavlović, Neda and Jakovetić Tanasković, Sonja and Knežević-Jugović, Zorica",
year = "2022",
abstract = "Proteins are an essential component of the human diet and effectively are provided from
both animal and plant sources. Sourcing ribulose-1,5-bisphosphate carboxylase/oxygenase
(RuBisCO) from green biomass such as pumpkin leaves, a by-product of the oil industry,
is a promising tool for obtaining protein with an excellent nutritional profile under the
green biorafinery concept, that can be used as a substitute for animal proteins. This study
provides the results regarding the implementation of ultrasound-assisted processing (UAP)
in the protein extraction from pumpkin leaves. UAP for recovery of RuBisCO proteins was
optimized by varing the solvent-to-sample ratio, solvent pH, ultrasound amplitudes and
time. UAP appears to have a strong potential for recovering RuBisCO protein from
pumpkin leaves, as it was highly influenced by the solvent-to-sample ratio along with UAP
amplitude and time., Proteini životinjskog i biljnog porekla čine osnovne namirnice ljudske ishrane. Prema
konceptu zelene biorafinerije, izolovanje RuBisCO proteina (ribuloza-1,5-bisfosfat
karboksilaza/oksigenaze) iz zelene biomase kakva je lišće bundeve, nusproizvod industrije
prerade uljarica, je obećavajući pristup za dobijanje proteina sa visokom nutritivnom
vrednošću koje je moguće koristiti kao zamenu za proteine životinjskog porekla. U ovom
radu, ultrazvučno potpomognutim procesom ekstrakcije izolovani su proteini iz lišća
bundeve. Ultrazvučni proces ekstrakcije RuBisCO proteina optimizovan je variranjem
odnosa rastvarač-uzorak, pH rastvarača, vremena trajanja i amplitude ultrazvuka.
Ultrazvuk je ispoljio veliki pozitivan efekat na izolovanje RuBisCO proteina iz lišća
bundeve, na šta je u najvećoj meri tokom optimizacije procesa uticao odnos rastvaračuzorak, kao i amplituda i vreme ultrazvuka.",
publisher = "Beograd : Srpsko hemijsko društvo = Serbian Chemical Society",
journal = "Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade",
title = "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6252"
}

Mijalković, J., Šekuljica, N., Biçer, F., Pavlović, N., Jakovetić Tanasković, S.,& Knežević-Jugović, Z.. (2022). Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves. in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade
Beograd : Srpsko hemijsko društvo = Serbian Chemical Society., 174-174.
https://hdl.handle.net/21.15107/rcub_technorep_6252

Mijalković J, Šekuljica N, Biçer F, Pavlović N, Jakovetić Tanasković S, Knežević-Jugović Z. Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves. in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade. 2022;:174-174.
https://hdl.handle.net/21.15107/rcub_technorep_6252 .

Mijalković, Jelena, Šekuljica, Nataša, Biçer, Furkan, Pavlović, Neda, Jakovetić Tanasković, Sonja, Knežević-Jugović, Zorica, "Ultrasound-assisted extraction process for recovery of RuBisCO proteins from pumpkin leaves" in Book of Abstracts - Proceedings / 58th meeting of the Serbian Chemical Society, Belgrade (2022):174-174,
https://hdl.handle.net/21.15107/rcub_technorep_6252 .