@article{
author = "Klaus, Anita and Kozarski, Maja and Vunduk, Jovana and Petrović, Predrag and Nikšić, Miomir",
year = "2016",
abstract = "Hot water extract (AN), partially purified polysaccharides (AP) and hot alkali extract (ANa) obtained from wild mushroom G. applanatum were examined for their antibacterial and antifungal activity. Five Gram negative (Proteus hauseri, Escherichia coli O157:H7, Salmonella Enteritidis, Shigella sonnei, Yersinia enterocolitica), five Gram positive (Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Geobacillus stearothermophyllus, Enterococcus faecalis) bacterial strains, as well as two fungal strains (Candida albicans and Cryptococcus neoformans), all belonging to the American Type Culture Collection (ATCC), were tested by broth microdilution assay. In general, Gram-positive bacteria were more sensitive in the presence of tested extracts than Gram-negative bacterial species. Almost all tested extracts demonstrated a high microbistatic potential, and in most cases the microbicidal effect also has been reached. The best antibacterial effect of hot water alkali extract, ANa, was observed in the case of E. faecalis (MIC 0.039 mg/mL, MBC 1.25 mg/mL), while the same extract was the most effective antifungal agent towards C. neoformans (MIC 0.078 mg/mL, MFC 2.5 mg/mL). This research indicates possibility to use selected extracts, obtained from the mushroom G. applanatum, in order to prevent the development of some pathogenic microorganisms and food spoilage., Ispitivana je antibakterijska i antifungalna aktivnost vrelog vodenog ekstrakta (AN), parcijalno prečišćenog polisaharidnog ekstrakta (AP) i vrelog alkalnog ekstrakta, dobijenih iz G. applanatum, gljive iz prirode. Pet Gram negativnih (Proteus hauseri, Escherichia coli O157:H7, Salmonella Enteritidis, Shigella sonnei, Yersinia enterocolitica), pet Gram pozitivnih (Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Geobacillus stearothermophyllus, Enterococcus faecalis) bakterijskih vrsta, kao i dva soja gljiva (Candida albicans and Cryptococcus neoformans), od kojih svi pripadaju Američkoj tipskoj kolekciji kultura (ATCC), testirani su mikrodilucionom metodom u bujonu. Generelno, Gram pozitivne bakterije su bile mnogo osetljivije u prisustvu testiranih ekstrakata nego Gram negativne bakterijske vrste. Skoro svi testirani ekstrakti pokazali su visok mikrobistatički potencijal, a u najvećem broju slučajeva dostignut je i mikrobicidni efekat. Najbolji antibakterijski efekat vrelog alkalnog ekstrakta, ANa, uočen je u slučaju E. faecalis (MIC 0.039 mg/mL, MBC 1.25 mg/mL), dok je ovaj ekstrakt bio i najefikasniji antifungalni agens prema C. neoformans (MIC 0.078 mg/mL, MFC 2.5 mg/mL). Ovo istraživanje ukazuje na mogućnost korišćenja odabranih ekstrakata dobijenih iz gljive G. applanatum u cilju prevencije razvoja nekih patogenih mikroorganizama i kvarenja hrane.",
publisher = "Institute for Medicinal Plants Research "Dr. Josif Pancic"",
journal = "Lekovite sirovine",
title = "Antibacterial and antifungal potential of wild basidiomycete mushroom Ganoderma applanatum, Antibakterijski i antifungalni potencijal gljive Ganoderma applanatum bazidiomicete iz prirode",
pages = "46-37",
number = "36",
doi = "10.5937/leksir1636037K"
}