TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Carević, Tamara
AU  - Stojković, Dejan
AU  - Bugarski, Branko
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7073
AB  - Paeonia tenuifolia L. (steppe peony) petal extract was proficiently encapsulated into liposomes and biopolymer films in the current work, both times utilizing a single-step procedure. The encapsulation efficiency, size of the particles, and index of polydispersity (PDI), as well as the ζ potential of the obtained liposomes were determined, whereas in the case of films, the test included moisture content and mechanical property assessment. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical composition and existence of numerous interactions in the systems. All the obtained encapsulates were subjected to antibacterial, antifungal and antibiofilm activity testing of the pathogens associated with human skin. The results indicated that the liposomes prepared using Phospholipon had the highest encapsulation efficiency (72.04%), making them the most favorable ones in the release study as well. The biological assays also revealed that Phospholipon was the most beneficial phospholipid mixture for the preparation of liposomes, whereas the film containing these liposomes did not have the ability to inhibit pathogen growth, making the double encapsulation of P. tenuifolia L. petal extract needless. These findings may be a first step toward the potential use of steppe peony extract-loaded films and liposomes in pharmaceutical and cosmetical industries.
PB  - MDPI
T2  - Pharmaceutics
T1  - Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential
IS  - 12
SP  - 2742
VL  - 15
DO  - 10.3390/pharmaceutics15122742
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Carević, Tamara and Stojković, Dejan and Bugarski, Branko and Jovanović, Aleksandra A.",
year = "2023",
abstract = "Paeonia tenuifolia L. (steppe peony) petal extract was proficiently encapsulated into liposomes and biopolymer films in the current work, both times utilizing a single-step procedure. The encapsulation efficiency, size of the particles, and index of polydispersity (PDI), as well as the ζ potential of the obtained liposomes were determined, whereas in the case of films, the test included moisture content and mechanical property assessment. Fourier transform infrared spectroscopy (FT-IR) was used to evaluate the chemical composition and existence of numerous interactions in the systems. All the obtained encapsulates were subjected to antibacterial, antifungal and antibiofilm activity testing of the pathogens associated with human skin. The results indicated that the liposomes prepared using Phospholipon had the highest encapsulation efficiency (72.04%), making them the most favorable ones in the release study as well. The biological assays also revealed that Phospholipon was the most beneficial phospholipid mixture for the preparation of liposomes, whereas the film containing these liposomes did not have the ability to inhibit pathogen growth, making the double encapsulation of P. tenuifolia L. petal extract needless. These findings may be a first step toward the potential use of steppe peony extract-loaded films and liposomes in pharmaceutical and cosmetical industries.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential",
number = "12",
pages = "2742",
volume = "15",
doi = "10.3390/pharmaceutics15122742"
}

Čutović, N., Marković, T., Carević, T., Stojković, D., Bugarski, B.,& Jovanović, A. A.. (2023). Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential. in Pharmaceutics
MDPI., 15(12), 2742.
https://doi.org/10.3390/pharmaceutics15122742

Čutović N, Marković T, Carević T, Stojković D, Bugarski B, Jovanović AA. Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential. in Pharmaceutics. 2023;15(12):2742.
doi:10.3390/pharmaceutics15122742 .

Čutović, Natalija, Marković, Tatjana, Carević, Tamara, Stojković, Dejan, Bugarski, Branko, Jovanović, Aleksandra A., "Liposomal and Liposomes-Film Systems as Carriers for Bioactives from Paeonia tenuifolia L. Petals: Physicochemical Characterization and Biological Potential" in Pharmaceutics, 15, no. 12 (2023):2742,
https://doi.org/10.3390/pharmaceutics15122742 . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Marković, Tatjana
AU  - Kostić, Marina
AU  - Gašić, Uroš
AU  - Prijić, Željana
AU  - Ren, Xiuxia
AU  - Lukić, Milan
AU  - Bugarski, Branko
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5298
AB  - Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia
IS  - 12
SP  - 1537
VL  - 15
DO  - 10.3390/ph15121537
ER  - 

@article{
author = "Čutović, Natalija and Marković, Tatjana and Kostić, Marina and Gašić, Uroš and Prijić, Željana and Ren, Xiuxia and Lukić, Milan and Bugarski, Branko",
year = "2022",
abstract = "Without being aware of its chemical makeup, many ancient societies have used Steppe peony in their traditional medicine. Given that modern phytopreparation intended for use on human skin requires, above all, knowledge of its chemical composition, the goal of this study was to make a screening of the composition of aqueous and methanolic extracts of the petals of P. tenuifolia L. and to examine them for various skin-beneficial properties. The extracts were prepared by maceration, ultrasound-assisted, and microwave-assisted extraction procedures. The chemical profiling was conducted by the use of UHPLC-LTQ-OrbiTrap MS and UHPLC/MS, and spectrophotometric methods for the determination of total polyphenol and total flavonoid contents. The biological activities entailed antioxidant ABTS, DPPH, CUPRAC (Cupric Ion Reducing Antioxidant Capacity), and FRAP (Ferric Reducing Antioxidant Power) assays, antimicrobial (antibacterial and antifungal) and antibiofilm activities, cytotoxicity, wound healing potential, as well as the adhesion and invasion of Staphylococcus lugdunensis. The results showed that the petals are rich in phenolic acids and flavonoids, which are commonly associated with numerous biological activities. The aqueous extracts were more efficient in the majority of the bioactivity assays then the methanolic ones, whereas the optimal extraction method varied between the assays. This study is the first step towards the safe use of the aqueous extracts of P. tenuifolia petals for therapeutic skin treatments.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia",
number = "12",
pages = "1537",
volume = "15",
doi = "10.3390/ph15121537"
}

Čutović, N., Marković, T., Kostić, M., Gašić, U., Prijić, Ž., Ren, X., Lukić, M.,& Bugarski, B.. (2022). Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals
MDPI., 15(12), 1537.
https://doi.org/10.3390/ph15121537

Čutović N, Marković T, Kostić M, Gašić U, Prijić Ž, Ren X, Lukić M, Bugarski B. Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia. in Pharmaceuticals. 2022;15(12):1537.
doi:10.3390/ph15121537 .

Čutović, Natalija, Marković, Tatjana, Kostić, Marina, Gašić, Uroš, Prijić, Željana, Ren, Xiuxia, Lukić, Milan, Bugarski, Branko, "Chemical Profile and Skin-Beneficial Activities of the Petal Extracts of Paeonia tenuifolia L. from Serbia" in Pharmaceuticals, 15, no. 12 (2022):1537,
https://doi.org/10.3390/ph15121537 . .