TY  - JOUR
AU  - Bačić, Ana
AU  - Milivojević, Vladimir
AU  - Petković, Isidora
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - Medić Brkić, Branislava
AU  - Popadić, Dušan
AU  - Milosavljević, Tomica
AU  - Rajilić-Stojanović, Mirjana
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6054
AB  - Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.
T2  - Journal of Fungi
T1  - In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species
IS  - 3
SP  - 328
VL  - 9
DO  - 10.3390/jof9030328
ER  - 

@article{
author = "Bačić, Ana and Milivojević, Vladimir and Petković, Isidora and Kekić, Dušan and Gajić, Ina and Medić Brkić, Branislava and Popadić, Dušan and Milosavljević, Tomica and Rajilić-Stojanović, Mirjana",
year = "2023",
abstract = "Helicobacter pylori eradication is characterized by decreasing successful eradication rates. Although treatment failure is primarily associated with resistance to antibiotics, other unknown factors may influence the eradication outcome. This study aimed to assess the presence of the antibiotics resistance genes in H. pylori and the presence of Candida spp., which are proposed to be endosymbiotic hosts of H. pylori, in gastric biopsies of H. pylori-positive patients while simultaneously assessing their relationship. The detection and identification of Candida yeasts and the detection of mutations specific for clarithromycin and fluoroquinolones were performed by using the real-time PCR (RT-PCR) method on DNA extracted from 110 gastric biopsy samples of H. pylori-positive participants. Resistance rate to clarithromycin and fluoroquinolone was 52% and 47%, respectively. Antibiotic resistance was associated with more eradication attempts (p < 0.05). Candida species were detected in nine (8.18%) patients. Candida presence was associated with older age (p < 0.05). A high rate of antibiotic resistance was observed, while Candida presence was scarce, suggesting that endosymbiosis between H. pylori and Candida may not be a major contributing factor to the eradication failure. However, the older age favored Candida gastric mucosa colonization, which could contribute to gastric pathologies and microbiome dysbiosis.",
journal = "Journal of Fungi",
title = "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species",
number = "3",
pages = "328",
volume = "9",
doi = "10.3390/jof9030328"
}

Bačić, A., Milivojević, V., Petković, I., Kekić, D., Gajić, I., Medić Brkić, B., Popadić, D., Milosavljević, T.,& Rajilić-Stojanović, M.. (2023). In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi, 9(3), 328.
https://doi.org/10.3390/jof9030328

Bačić A, Milivojević V, Petković I, Kekić D, Gajić I, Medić Brkić B, Popadić D, Milosavljević T, Rajilić-Stojanović M. In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species. in Journal of Fungi. 2023;9(3):328.
doi:10.3390/jof9030328 .

Bačić, Ana, Milivojević, Vladimir, Petković, Isidora, Kekić, Dušan, Gajić, Ina, Medić Brkić, Branislava, Popadić, Dušan, Milosavljević, Tomica, Rajilić-Stojanović, Mirjana, "In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species" in Journal of Fungi, 9, no. 3 (2023):328,
https://doi.org/10.3390/jof9030328 . .

TY  - JOUR
AU  - Assaleh, Mohamed H.
AU  - Bjelogrlic, Snezana K.
AU  - Prlainović, Nevena
AU  - Cvijetić, Ilija
AU  - Bozic, Aleksandra
AU  - Aranđelović, Irena
AU  - Vukovic, Dragana
AU  - Marinković, Aleksandar
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4986
AB  - A series of twelve novel hybrids of cinnamic acid and thiocarbohydrazones were designed, synthesized in high yield using a simple coupling strategy via acid chlorides, and evaluated for their impact against Mycobacterium tuberculosis (Mtb) and cancer cells survival. Among them, compound 3 demonstrated strong anti-Mtb activity by reducing bacilli survival for>90 % in all three treated Mtb isolates, whereas isoniazid and rifampicin did not. Moreover, compound 3 didn't affect vitality of HepG-2 cells, implying on advantageous hepatotoxicity profile compared to current therapeutic options for tuberculosis. Compounds 2a and 3b displayed as strong inducers of apoptosis in A549 cells, both activating intrinsic caspase pathway and cell cycle arrest at the G0/ G1 phase. Subsequent analyses disclosed differences in their activities, where 3b has ability to induce production of mitochondrial superoxide anions, while 2a significantly inhibited cellular mobility. More importantly, 3b considerably affected viability of HepG-2 and HaCaT cells, whereas 2a had moderate impact only on the later. Molecular modeling studies indicated high permeability and good absorption through the human intestine, and moderate aqueous solubility with poor blood-brain barrier permeability. In summary, our results reveal that novel compounds 3 and 2a represent promising agents for tuberculosis and cancer treatment, respectively, indicating that fur-ther investigation needs to be performed to clarify the mechanisms of their anti-Mtb and anticancer activity. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
T2  - Arabian Journal of Chemistry
T1  - Antimycobacterial and anticancer activity of newly designed cinnamic acid hydrazides with favorable toxicity profile: Antimycobacterial and anticancer activity of newly designed cinnamic acid
IS  - 1
SP  - 103532
VL  - 15
DO  - 10.1016/j.arabjc.2021.103532
ER  - 

@article{
author = "Assaleh, Mohamed H. and Bjelogrlic, Snezana K. and Prlainović, Nevena and Cvijetić, Ilija and Bozic, Aleksandra and Aranđelović, Irena and Vukovic, Dragana and Marinković, Aleksandar",
year = "2022",
abstract = "A series of twelve novel hybrids of cinnamic acid and thiocarbohydrazones were designed, synthesized in high yield using a simple coupling strategy via acid chlorides, and evaluated for their impact against Mycobacterium tuberculosis (Mtb) and cancer cells survival. Among them, compound 3 demonstrated strong anti-Mtb activity by reducing bacilli survival for>90 % in all three treated Mtb isolates, whereas isoniazid and rifampicin did not. Moreover, compound 3 didn't affect vitality of HepG-2 cells, implying on advantageous hepatotoxicity profile compared to current therapeutic options for tuberculosis. Compounds 2a and 3b displayed as strong inducers of apoptosis in A549 cells, both activating intrinsic caspase pathway and cell cycle arrest at the G0/ G1 phase. Subsequent analyses disclosed differences in their activities, where 3b has ability to induce production of mitochondrial superoxide anions, while 2a significantly inhibited cellular mobility. More importantly, 3b considerably affected viability of HepG-2 and HaCaT cells, whereas 2a had moderate impact only on the later. Molecular modeling studies indicated high permeability and good absorption through the human intestine, and moderate aqueous solubility with poor blood-brain barrier permeability. In summary, our results reveal that novel compounds 3 and 2a represent promising agents for tuberculosis and cancer treatment, respectively, indicating that fur-ther investigation needs to be performed to clarify the mechanisms of their anti-Mtb and anticancer activity. (c) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).",
journal = "Arabian Journal of Chemistry",
title = "Antimycobacterial and anticancer activity of newly designed cinnamic acid hydrazides with favorable toxicity profile: Antimycobacterial and anticancer activity of newly designed cinnamic acid",
number = "1",
pages = "103532",
volume = "15",
doi = "10.1016/j.arabjc.2021.103532"
}

Assaleh, M. H., Bjelogrlic, S. K., Prlainović, N., Cvijetić, I., Bozic, A., Aranđelović, I., Vukovic, D.,& Marinković, A.. (2022). Antimycobacterial and anticancer activity of newly designed cinnamic acid hydrazides with favorable toxicity profile: Antimycobacterial and anticancer activity of newly designed cinnamic acid. in Arabian Journal of Chemistry, 15(1), 103532.
https://doi.org/10.1016/j.arabjc.2021.103532

Assaleh MH, Bjelogrlic SK, Prlainović N, Cvijetić I, Bozic A, Aranđelović I, Vukovic D, Marinković A. Antimycobacterial and anticancer activity of newly designed cinnamic acid hydrazides with favorable toxicity profile: Antimycobacterial and anticancer activity of newly designed cinnamic acid. in Arabian Journal of Chemistry. 2022;15(1):103532.
doi:10.1016/j.arabjc.2021.103532 .

Assaleh, Mohamed H., Bjelogrlic, Snezana K., Prlainović, Nevena, Cvijetić, Ilija, Bozic, Aleksandra, Aranđelović, Irena, Vukovic, Dragana, Marinković, Aleksandar, "Antimycobacterial and anticancer activity of newly designed cinnamic acid hydrazides with favorable toxicity profile: Antimycobacterial and anticancer activity of newly designed cinnamic acid" in Arabian Journal of Chemistry, 15, no. 1 (2022):103532,
https://doi.org/10.1016/j.arabjc.2021.103532 . .