TY  - JOUR
AU  - Milovanović, Ivan
AU  - Zengin, Gokhan
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/17
AB  - Background This study provides an insight into the impact of ultrasound-assisted extraction with water as solvent (UAEW) and extraction by supercritical carbon dioxide (SC-CO2) with 5% EtOH on antioxidant and enzyme inhibitory activity in regard to the chemical profile of the edible and medicinal mushroom,Pleurotus pulmonarius. Results Extraction efficiency was between 0.36% and 63.32%, depending on the extraction technique. The main compounds in the extracts were fatty acids. Supercritical CO(2)extraction with co-solvent was the most suitable method for obtaining extracts that were rich in ergosterol content, reaching a value of 40.1 mg g(-1). The UAEW of crude mushroom powder ensured the highest yield, as well as the extracts with best antioxidative activity. The measurements of enzyme inhibitory activity revealed that all types of investigated extracts exhibited only tyrosinase and amylase inhibition at a significant level. Conclusion Based on our results, the extraction methods significantly affected the chemical profile and bioactivity ofP. pulmonarius.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties
EP  - 2293
IS  - 6
SP  - 2284
VL  - 101
DO  - 10.1002/jsfa.10849
ER  - 

@article{
author = "Milovanović, Ivan and Zengin, Gokhan and Maksimović, Svetolik and Tadić, Vanja",
year = "2021",
abstract = "Background This study provides an insight into the impact of ultrasound-assisted extraction with water as solvent (UAEW) and extraction by supercritical carbon dioxide (SC-CO2) with 5% EtOH on antioxidant and enzyme inhibitory activity in regard to the chemical profile of the edible and medicinal mushroom,Pleurotus pulmonarius. Results Extraction efficiency was between 0.36% and 63.32%, depending on the extraction technique. The main compounds in the extracts were fatty acids. Supercritical CO(2)extraction with co-solvent was the most suitable method for obtaining extracts that were rich in ergosterol content, reaching a value of 40.1 mg g(-1). The UAEW of crude mushroom powder ensured the highest yield, as well as the extracts with best antioxidative activity. The measurements of enzyme inhibitory activity revealed that all types of investigated extracts exhibited only tyrosinase and amylase inhibition at a significant level. Conclusion Based on our results, the extraction methods significantly affected the chemical profile and bioactivity ofP. pulmonarius.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties",
pages = "2293-2284",
number = "6",
volume = "101",
doi = "10.1002/jsfa.10849"
}

Milovanović, I., Zengin, G., Maksimović, S.,& Tadić, V.. (2021). Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 101(6), 2284-2293.
https://doi.org/10.1002/jsfa.10849

Milovanović I, Zengin G, Maksimović S, Tadić V. Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties. in Journal of the Science of Food and Agriculture. 2021;101(6):2284-2293.
doi:10.1002/jsfa.10849 .

Milovanović, Ivan, Zengin, Gokhan, Maksimović, Svetolik, Tadić, Vanja, "Supercritical and ultrasound-assisted extracts fromPleurotus pulmonariusmushroom: chemical profiles, antioxidative, and enzyme-inhibitory properties" in Journal of the Science of Food and Agriculture, 101, no. 6 (2021):2284-2293,
https://doi.org/10.1002/jsfa.10849 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Vulić, Jelena
AU  - Ivanović, Jasna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4561
AB  - Present study was aimed to produce biodegradable films with antioxidant activity for active food packaging. Films of poly(lactic acid) (PLA) with 5 wt% of poly(ε-caprolactone) (PCL), denoted as PLA-5, were loaded with thymol, carvacrol and their mixture using supercritical CO2 at 40 °C and 10 MPa for 5 h, with 27.53, 21.18, and 21.49 % of loading achieved, respectively. Morphological, structural and mechanical properties of the obtained PLA-5 films were analyzed. The highest total polyphenol content and the best antioxidant activity as well as good storage stability, acceptable physical properties and the lowest release rate (44.51 mg/L released after 6 weeks) were observed for PLA-5 film loaded with thymol and carvacrol mixture. Mixture of thymol and carvacrol loaded into PLA-5 film showed synergy and low percentage of migration into distilled water, revealing great potential for application as a food packaging material with aim to decrease requirements for amount of the active ingredients.
T2  - Food Packaging and Shelf Life
T1  - Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO2 for active food packaging
VL  - 26
DO  - 10.1016/j.fpsl.2020.100578
ER  - 

@article{
author = "Lukić, Ivana and Vulić, Jelena and Ivanović, Jasna",
year = "2020",
abstract = "Present study was aimed to produce biodegradable films with antioxidant activity for active food packaging. Films of poly(lactic acid) (PLA) with 5 wt% of poly(ε-caprolactone) (PCL), denoted as PLA-5, were loaded with thymol, carvacrol and their mixture using supercritical CO2 at 40 °C and 10 MPa for 5 h, with 27.53, 21.18, and 21.49 % of loading achieved, respectively. Morphological, structural and mechanical properties of the obtained PLA-5 films were analyzed. The highest total polyphenol content and the best antioxidant activity as well as good storage stability, acceptable physical properties and the lowest release rate (44.51 mg/L released after 6 weeks) were observed for PLA-5 film loaded with thymol and carvacrol mixture. Mixture of thymol and carvacrol loaded into PLA-5 film showed synergy and low percentage of migration into distilled water, revealing great potential for application as a food packaging material with aim to decrease requirements for amount of the active ingredients.",
journal = "Food Packaging and Shelf Life",
title = "Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO2 for active food packaging",
volume = "26",
doi = "10.1016/j.fpsl.2020.100578"
}

Lukić, I., Vulić, J.,& Ivanović, J.. (2020). Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO2 for active food packaging. in Food Packaging and Shelf Life, 26.
https://doi.org/10.1016/j.fpsl.2020.100578

Lukić I, Vulić J, Ivanović J. Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO2 for active food packaging. in Food Packaging and Shelf Life. 2020;26.
doi:10.1016/j.fpsl.2020.100578 .

Lukić, Ivana, Vulić, Jelena, Ivanović, Jasna, "Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO2 for active food packaging" in Food Packaging and Shelf Life, 26 (2020),
https://doi.org/10.1016/j.fpsl.2020.100578 . .

TY  - JOUR
AU  - Lukić, Ivana
AU  - Vulic, Jelena
AU  - Ivanovic, Jasna
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4736
AB  - Present study was aimed to produce biodegradable films with antioxidant activity for active food packaging. Films of poly(lactic acid) (PLA) with 5 wt% of poly(epsilon-caprolactone) (PCL), denoted as PLA-5, were loaded with thymol, carvacrol and their mixture using supercritical CO2 at 40 degrees C and 10 MPa for 5 h, with 27.53, 21.18, and 21.49 % of loading achieved, respectively. Morphological, structural and mechanical properties of the obtained PLA-5 films were analyzed. The highest total polyphenol content and the best antioxidant activity as well as good storage stability, acceptable physical properties and the lowest release rate (44.51 mg/L released after 6 weeks) were observed for PLA-5 film loaded with thymol and carvacrol mixture. Mixture of thymol and carvacrol loaded into PLA-5 film showed synergy and low percentage of migration into distilled water, revealing great potential for application as a food packaging material with aim to decrease requirements for amount of the active ingredients.
T2  - Food Packaging and Shelf Life
T1  - Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO(2) for active food packaging
VL  - 26
DO  - 10.1016/j.fpsl.2020.100578
ER  - 

@article{
author = "Lukić, Ivana and Vulic, Jelena and Ivanovic, Jasna",
year = "2020",
abstract = "Present study was aimed to produce biodegradable films with antioxidant activity for active food packaging. Films of poly(lactic acid) (PLA) with 5 wt% of poly(epsilon-caprolactone) (PCL), denoted as PLA-5, were loaded with thymol, carvacrol and their mixture using supercritical CO2 at 40 degrees C and 10 MPa for 5 h, with 27.53, 21.18, and 21.49 % of loading achieved, respectively. Morphological, structural and mechanical properties of the obtained PLA-5 films were analyzed. The highest total polyphenol content and the best antioxidant activity as well as good storage stability, acceptable physical properties and the lowest release rate (44.51 mg/L released after 6 weeks) were observed for PLA-5 film loaded with thymol and carvacrol mixture. Mixture of thymol and carvacrol loaded into PLA-5 film showed synergy and low percentage of migration into distilled water, revealing great potential for application as a food packaging material with aim to decrease requirements for amount of the active ingredients.",
journal = "Food Packaging and Shelf Life",
title = "Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO(2) for active food packaging",
volume = "26",
doi = "10.1016/j.fpsl.2020.100578"
}

Lukić, I., Vulic, J.,& Ivanovic, J.. (2020). Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO(2) for active food packaging. in Food Packaging and Shelf Life, 26.
https://doi.org/10.1016/j.fpsl.2020.100578

Lukić I, Vulic J, Ivanovic J. Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO(2) for active food packaging. in Food Packaging and Shelf Life. 2020;26.
doi:10.1016/j.fpsl.2020.100578 .

Lukić, Ivana, Vulic, Jelena, Ivanovic, Jasna, "Antioxidant activity of PLA/PCL films loaded with thymol and/or carvacrol using scCO(2) for active food packaging" in Food Packaging and Shelf Life, 26 (2020),
https://doi.org/10.1016/j.fpsl.2020.100578 . .

TY  - JOUR
AU  - Bogdanović, Aleksandra
AU  - Tadić, Vanja
AU  - Petrović, Slobodan
AU  - Skala, Dejan
PY  - 2020
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4543
AB  - Supercritical CO2 extraction was applied on fenugreek (Trigonella foenum-graecum L., Fabaceae) seeds with the aim to define optimal process conditions to obtain the maximal extract yields content of steroidal sapogenins. Central composite rotatable design (CCRD) combined with response surface methodology (RSM) was applied to determine optimal process conditions defined by the influence and interaction of pressure, temperature and time of extraction through consumption of SC CO2. Optimization experiments revealed that the pressure of 24.73 MPa, the temperature of 38.2 degrees C and the consumption of SC CO2 of 19.24 g/g(DM) were the best process conditions enabling the maximal yield of extract and gain of the highest content of sapogenins. The optimal values of pressure and temperature defined SC CO2 density of 885.47 kg/m(3), which provided the maximal yield of the extract with the highest content of steroidal sapogenins. The achieved yield of extract at these conditions was 0.073g/g(DM), with 0.774 mg/g(DM) diosgenin, 0.477 mg/g(DM) protodioscin, 0.713 mg/g(DM) sarsapogenin and 0.205 mg/g(DM) oleanolic and ursolic acid with a significant quantity of 2.475 mg/g(DM) in the obtained extract.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed
EP  - 182
IS  - 2
SP  - 171
VL  - 26
DO  - 10.2298/CICEQ191001036B
ER  - 

@article{
author = "Bogdanović, Aleksandra and Tadić, Vanja and Petrović, Slobodan and Skala, Dejan",
year = "2020",
abstract = "Supercritical CO2 extraction was applied on fenugreek (Trigonella foenum-graecum L., Fabaceae) seeds with the aim to define optimal process conditions to obtain the maximal extract yields content of steroidal sapogenins. Central composite rotatable design (CCRD) combined with response surface methodology (RSM) was applied to determine optimal process conditions defined by the influence and interaction of pressure, temperature and time of extraction through consumption of SC CO2. Optimization experiments revealed that the pressure of 24.73 MPa, the temperature of 38.2 degrees C and the consumption of SC CO2 of 19.24 g/g(DM) were the best process conditions enabling the maximal yield of extract and gain of the highest content of sapogenins. The optimal values of pressure and temperature defined SC CO2 density of 885.47 kg/m(3), which provided the maximal yield of the extract with the highest content of steroidal sapogenins. The achieved yield of extract at these conditions was 0.073g/g(DM), with 0.774 mg/g(DM) diosgenin, 0.477 mg/g(DM) protodioscin, 0.713 mg/g(DM) sarsapogenin and 0.205 mg/g(DM) oleanolic and ursolic acid with a significant quantity of 2.475 mg/g(DM) in the obtained extract.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed",
pages = "182-171",
number = "2",
volume = "26",
doi = "10.2298/CICEQ191001036B"
}

Bogdanović, A., Tadić, V., Petrović, S.,& Skala, D.. (2020). Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 26(2), 171-182.
https://doi.org/10.2298/CICEQ191001036B

Bogdanović A, Tadić V, Petrović S, Skala D. Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed. in Chemical Industry & Chemical Engineering Quarterly. 2020;26(2):171-182.
doi:10.2298/CICEQ191001036B .

Bogdanović, Aleksandra, Tadić, Vanja, Petrović, Slobodan, Skala, Dejan, "Supercritical CO2 extraction of steroidal sapogenins from fenugreek (tngonella foenum-graecum l.) Seed" in Chemical Industry & Chemical Engineering Quarterly, 26, no. 2 (2020):171-182,
https://doi.org/10.2298/CICEQ191001036B . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Đuriš, Jelena
AU  - Dapčević, Aleksandra
AU  - Medarević, Đorđe
AU  - Ibrić, Svetlana
AU  - Žižović, Irena
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4074
AB  - The present work is aimed towards designing advanced materials by means of sustainable processes. In that sense, the utilization of supercritical CO2 (scCO(2)) for processing of pharmaceutical polymers (Soluplus (R), Eudragit (R), and hydroxypropyl methylcellulose acetate succinate), alone and with an addition of cardiovascular drug Carvedilol, was explored. Employed single-step static scCO(2) process (pressure of 30 MPa and temperature of 100 degrees C for 2 h) enabled fabrication of solvent-free polymeric foams and Carvedilol solid dispersions with controlled microstructure and average pore diameter of 101-257 pm suitable for application in the pharmaceutical industry. ScCO2 did not remain in the foams after processing or affected the polymer composition, while Carvedilol formed hydrogen bonds with the polymers. Carvedilol was molecularly dispersed in the fabricated solid dispersions and its transition from the crystalline to amorphous form was complete. Korsmeyer-Peppas model was successfully used for the mathematical description of Carvedilol dissolution from solid dispersions. The dissolution rate of Carvedilol in acidic medium was significantly enhanced by its dispersion in tested polymers using the proposed high-pressure method.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Testing
T1  - Soluplus (R), Eudragit (R), HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO2 process
EP  - 64
SP  - 54
VL  - 76
DO  - 10.1016/j.polymertesting.2019.03.001
ER  - 

@article{
author = "Milovanović, Stoja and Đuriš, Jelena and Dapčević, Aleksandra and Medarević, Đorđe and Ibrić, Svetlana and Žižović, Irena",
year = "2019",
abstract = "The present work is aimed towards designing advanced materials by means of sustainable processes. In that sense, the utilization of supercritical CO2 (scCO(2)) for processing of pharmaceutical polymers (Soluplus (R), Eudragit (R), and hydroxypropyl methylcellulose acetate succinate), alone and with an addition of cardiovascular drug Carvedilol, was explored. Employed single-step static scCO(2) process (pressure of 30 MPa and temperature of 100 degrees C for 2 h) enabled fabrication of solvent-free polymeric foams and Carvedilol solid dispersions with controlled microstructure and average pore diameter of 101-257 pm suitable for application in the pharmaceutical industry. ScCO2 did not remain in the foams after processing or affected the polymer composition, while Carvedilol formed hydrogen bonds with the polymers. Carvedilol was molecularly dispersed in the fabricated solid dispersions and its transition from the crystalline to amorphous form was complete. Korsmeyer-Peppas model was successfully used for the mathematical description of Carvedilol dissolution from solid dispersions. The dissolution rate of Carvedilol in acidic medium was significantly enhanced by its dispersion in tested polymers using the proposed high-pressure method.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Testing",
title = "Soluplus (R), Eudragit (R), HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO2 process",
pages = "64-54",
volume = "76",
doi = "10.1016/j.polymertesting.2019.03.001"
}

Milovanović, S., Đuriš, J., Dapčević, A., Medarević, Đ., Ibrić, S.,& Žižović, I.. (2019). Soluplus (R), Eudragit (R), HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO2 process. in Polymer Testing
Elsevier Sci Ltd, Oxford., 76, 54-64.
https://doi.org/10.1016/j.polymertesting.2019.03.001

Milovanović S, Đuriš J, Dapčević A, Medarević Đ, Ibrić S, Žižović I. Soluplus (R), Eudragit (R), HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO2 process. in Polymer Testing. 2019;76:54-64.
doi:10.1016/j.polymertesting.2019.03.001 .

Milovanović, Stoja, Đuriš, Jelena, Dapčević, Aleksandra, Medarević, Đorđe, Ibrić, Svetlana, Žižović, Irena, "Soluplus (R), Eudragit (R), HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO2 process" in Polymer Testing, 76 (2019):54-64,
https://doi.org/10.1016/j.polymertesting.2019.03.001 . .

TY  - JOUR
AU  - Đuriš, Jelena
AU  - Milovanović, Stoja
AU  - Medarević, Đorđe
AU  - Dobričić, Vladimir
AU  - Dapčević, Aleksandra
AU  - Ibrić, Svetlana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4335
AB  - Solid dispersions production is one of the substantial approaches for improvement of poor drug solubility. Additionally, supercritical fluid assisted method for preparation of solid dispersions can offer many advantages in comparison to the conventional melting or solvent-evaporation methods. Miscibility analysis provides valuable guidance for selection of the most appropriate polymeric carrier for dispersion of the drug of interest. In addition to the increased drug release rate, solid dispersions should have proper mechanical attributes in order to be successfully formulated in the final solid dosage form such as tablet. Therefore, several pharmaceutical grade polymers have been selected for development of BCS Class II drug carvedilol (CARV) solid dispersions. They were compared based on behavior in supercritical CO2 and affinity towards CARV calculated from the miscibility analysis. By utilization of the supercritical CO2 assisted method, solid dispersions of CARV with the selected (co) polymers (polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), Soluplus (R) and Eudragit (R)) were obtained. Properties of the prepared CARV-polymer dispersions were observed by the polarizing and scanning electron microscopy and analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. CARV was additionally characterized by X-ray powder diffraction. Furthermore, in vitro dissolution studies and dynamic compaction analysis were performed on the selected samples of solid dispersions. Among the studied polymers, PVP and HPMC have been identified as polymers with the highest affinity towards CARV, based on the calculated delta(p) values. This has been also confirmed with the highest dissolution efficiency of CARV-PVP and CARV-HPMC solid dispersions. Solid state characterization indicated that CARV was dispersed either molecularly, or in the amorphous form, depending on interactions with each polymer. Determination of CARV-PVP and CARV-HPMC mechanical properties revealed that CARV-PVP solid dispersion has superior compactibility and tabletability. Therefore, CARV-PVP solid dispersion has been highlighted as the most appropriate for the further development of tablets as the final dosage form. Presented study provides an example for efficient approach for development of poorly soluble drug solid dispersion with satisfactory tableting properties.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol solid dispersions
EP  - 200
SP  - 190
VL  - 554
DO  - 10.1016/j.ijpharm.2018.11.015
ER  - 

@article{
author = "Đuriš, Jelena and Milovanović, Stoja and Medarević, Đorđe and Dobričić, Vladimir and Dapčević, Aleksandra and Ibrić, Svetlana",
year = "2019",
abstract = "Solid dispersions production is one of the substantial approaches for improvement of poor drug solubility. Additionally, supercritical fluid assisted method for preparation of solid dispersions can offer many advantages in comparison to the conventional melting or solvent-evaporation methods. Miscibility analysis provides valuable guidance for selection of the most appropriate polymeric carrier for dispersion of the drug of interest. In addition to the increased drug release rate, solid dispersions should have proper mechanical attributes in order to be successfully formulated in the final solid dosage form such as tablet. Therefore, several pharmaceutical grade polymers have been selected for development of BCS Class II drug carvedilol (CARV) solid dispersions. They were compared based on behavior in supercritical CO2 and affinity towards CARV calculated from the miscibility analysis. By utilization of the supercritical CO2 assisted method, solid dispersions of CARV with the selected (co) polymers (polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), Soluplus (R) and Eudragit (R)) were obtained. Properties of the prepared CARV-polymer dispersions were observed by the polarizing and scanning electron microscopy and analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. CARV was additionally characterized by X-ray powder diffraction. Furthermore, in vitro dissolution studies and dynamic compaction analysis were performed on the selected samples of solid dispersions. Among the studied polymers, PVP and HPMC have been identified as polymers with the highest affinity towards CARV, based on the calculated delta(p) values. This has been also confirmed with the highest dissolution efficiency of CARV-PVP and CARV-HPMC solid dispersions. Solid state characterization indicated that CARV was dispersed either molecularly, or in the amorphous form, depending on interactions with each polymer. Determination of CARV-PVP and CARV-HPMC mechanical properties revealed that CARV-PVP solid dispersion has superior compactibility and tabletability. Therefore, CARV-PVP solid dispersion has been highlighted as the most appropriate for the further development of tablets as the final dosage form. Presented study provides an example for efficient approach for development of poorly soluble drug solid dispersion with satisfactory tableting properties.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol solid dispersions",
pages = "200-190",
volume = "554",
doi = "10.1016/j.ijpharm.2018.11.015"
}

Đuriš, J., Milovanović, S., Medarević, Đ., Dobričić, V., Dapčević, A.,& Ibrić, S.. (2019). Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol solid dispersions. in International Journal of Pharmaceutics
Elsevier Science Bv, Amsterdam., 554, 190-200.
https://doi.org/10.1016/j.ijpharm.2018.11.015

Đuriš J, Milovanović S, Medarević Đ, Dobričić V, Dapčević A, Ibrić S. Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol solid dispersions. in International Journal of Pharmaceutics. 2019;554:190-200.
doi:10.1016/j.ijpharm.2018.11.015 .

Đuriš, Jelena, Milovanović, Stoja, Medarević, Đorđe, Dobričić, Vladimir, Dapčević, Aleksandra, Ibrić, Svetlana, "Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol solid dispersions" in International Journal of Pharmaceutics, 554 (2019):190-200,
https://doi.org/10.1016/j.ijpharm.2018.11.015 . .

TY  - JOUR
AU  - Djuris, Jelena
AU  - Milovanovic, Stoja
AU  - Medarevic, Djordje
AU  - Dobricic, Vladimir
AU  - Dapčević, Aleksandra
AU  - Ibric, Svetlana
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5030
AB  - Solid dispersions production is one of the substantial approaches for improvement of poor drug solubility. Additionally, supercritical fluid assisted method for preparation of solid dispersions can offer many advantages in comparison to the conventional melting or solvent-evaporation methods. Miscibility analysis provides valuable guidance for selection of the most appropriate polymeric carrier for dispersion of the drug of interest. In addition to the increased drug release rate, solid dispersions should have proper mechanical attributes in order to be successfully formulated in the final solid dosage form such as tablet. Therefore, several pharmaceutical grade polymers have been selected for development of BCS Class II drug carvedilol (CARV) solid dispersions. They were compared based on behavior in supercritical CO 2 and affinity towards CARV calculated from the miscibility analysis. By utilization of the supercritical CO 2 assisted method, solid dispersions of CARV with the selected (co)polymers (polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), Soluplus® and Eudragit®) were obtained. Properties of the prepared CARV-polymer dispersions were observed by the polarizing and scanning electron microscopy and analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. CARV was additionally characterized by X-ray powder diffraction. Furthermore, in vitro dissolution studies and dynamic compaction analysis were performed on the selected samples of solid dispersions. Among the studied polymers, PVP and HPMC have been identified as polymers with the highest affinity towards CARV, based on the calculated δ p values. This has been also confirmed with the highest dissolution efficiency of CARV-PVP and CARV-HPMC solid dispersions. Solid state characterization indicated that CARV was dispersed either molecularly, or in the amorphous form, depending on interactions with each polymer. Determination of CARV-PVP and CARV-HPMC mechanical properties revealed that CARV-PVP solid dispersion has superior compactibility and tabletability. Therefore, CARV-PVP solid dispersion has been highlighted as the most appropriate for the further development of tablets as the final dosage form. Presented study provides an example for efficient approach for development of poorly soluble drug solid dispersion with satisfactory tableting properties.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions
EP  - 200
SP  - 190
VL  - 554
DO  - 10.1016/j.ijpharm.2018.11.015
ER  - 

@article{
author = "Djuris, Jelena and Milovanovic, Stoja and Medarevic, Djordje and Dobricic, Vladimir and Dapčević, Aleksandra and Ibric, Svetlana",
year = "2019",
abstract = "Solid dispersions production is one of the substantial approaches for improvement of poor drug solubility. Additionally, supercritical fluid assisted method for preparation of solid dispersions can offer many advantages in comparison to the conventional melting or solvent-evaporation methods. Miscibility analysis provides valuable guidance for selection of the most appropriate polymeric carrier for dispersion of the drug of interest. In addition to the increased drug release rate, solid dispersions should have proper mechanical attributes in order to be successfully formulated in the final solid dosage form such as tablet. Therefore, several pharmaceutical grade polymers have been selected for development of BCS Class II drug carvedilol (CARV) solid dispersions. They were compared based on behavior in supercritical CO 2 and affinity towards CARV calculated from the miscibility analysis. By utilization of the supercritical CO 2 assisted method, solid dispersions of CARV with the selected (co)polymers (polyvinylpyrrolidone (PVP), hydroxypropyl methylcellulose (HPMC), Soluplus® and Eudragit®) were obtained. Properties of the prepared CARV-polymer dispersions were observed by the polarizing and scanning electron microscopy and analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. CARV was additionally characterized by X-ray powder diffraction. Furthermore, in vitro dissolution studies and dynamic compaction analysis were performed on the selected samples of solid dispersions. Among the studied polymers, PVP and HPMC have been identified as polymers with the highest affinity towards CARV, based on the calculated δ p values. This has been also confirmed with the highest dissolution efficiency of CARV-PVP and CARV-HPMC solid dispersions. Solid state characterization indicated that CARV was dispersed either molecularly, or in the amorphous form, depending on interactions with each polymer. Determination of CARV-PVP and CARV-HPMC mechanical properties revealed that CARV-PVP solid dispersion has superior compactibility and tabletability. Therefore, CARV-PVP solid dispersion has been highlighted as the most appropriate for the further development of tablets as the final dosage form. Presented study provides an example for efficient approach for development of poorly soluble drug solid dispersion with satisfactory tableting properties.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions",
pages = "200-190",
volume = "554",
doi = "10.1016/j.ijpharm.2018.11.015"
}

Djuris, J., Milovanovic, S., Medarevic, D., Dobricic, V., Dapčević, A.,& Ibric, S.. (2019). Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions. in International Journal of Pharmaceutics
Elsevier B.V.., 554, 190-200.
https://doi.org/10.1016/j.ijpharm.2018.11.015

Djuris J, Milovanovic S, Medarevic D, Dobricic V, Dapčević A, Ibric S. Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions. in International Journal of Pharmaceutics. 2019;554:190-200.
doi:10.1016/j.ijpharm.2018.11.015 .

Djuris, Jelena, Milovanovic, Stoja, Medarevic, Djordje, Dobricic, Vladimir, Dapčević, Aleksandra, Ibric, Svetlana, "Selection of the suitable polymer for supercritical fluid assisted preparation of carvedilol  solid dispersions" in International Journal of Pharmaceutics, 554 (2019):190-200,
https://doi.org/10.1016/j.ijpharm.2018.11.015 . .

TY  - JOUR
AU  - Kuska, Robert
AU  - Milovanović, Stoja
AU  - Frerich, Sulamith
AU  - Ivanović, Jasna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5041
AB  - Thermal properties of extrusion and injection grade polylactic acid (PLA) were analysed using high pressure differential scanning calorimetry (HP-DSC) under CO2 pressures of up to 50 MPa. The greatest depression of melting point and degree of crystallinity of the samples occurred at 20–30 MPa (∼97–115 °C). Batch and semi-continuous processes for supercritical foaming and impregnation of PLA with thymol or thyme extract were performed at 30 MPa and 100–110 °C to prevent thymol degradation, decrease heating requirements and ease polymer processing. At these conditions, PLA foams containing 5.6% or 1.1% of thymol and 0.7% of thyme extract were obtained using static or dynamic batch impregnation and semi-continuous extraction-impregnation process for 7 h, respectively. DSC and HP-DSC analyses revealed more pronounced effect of scCO2 plasticizing than foaming on PLA crystallinity. Neat and impregnated PLA foams with pores size of 15-200 μm have potential for food packaging, biomedical and insulation applications.
PB  - Elsevier B.V.
T2  - Journal of Supercritical Fluids
T1  - Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design
EP  - 80
SP  - 71
VL  - 144
DO  - 10.1016/j.supflu.2018.10.008
ER  - 

@article{
author = "Kuska, Robert and Milovanović, Stoja and Frerich, Sulamith and Ivanović, Jasna",
year = "2019",
abstract = "Thermal properties of extrusion and injection grade polylactic acid (PLA) were analysed using high pressure differential scanning calorimetry (HP-DSC) under CO2 pressures of up to 50 MPa. The greatest depression of melting point and degree of crystallinity of the samples occurred at 20–30 MPa (∼97–115 °C). Batch and semi-continuous processes for supercritical foaming and impregnation of PLA with thymol or thyme extract were performed at 30 MPa and 100–110 °C to prevent thymol degradation, decrease heating requirements and ease polymer processing. At these conditions, PLA foams containing 5.6% or 1.1% of thymol and 0.7% of thyme extract were obtained using static or dynamic batch impregnation and semi-continuous extraction-impregnation process for 7 h, respectively. DSC and HP-DSC analyses revealed more pronounced effect of scCO2 plasticizing than foaming on PLA crystallinity. Neat and impregnated PLA foams with pores size of 15-200 μm have potential for food packaging, biomedical and insulation applications.",
publisher = "Elsevier B.V.",
journal = "Journal of Supercritical Fluids",
title = "Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design",
pages = "80-71",
volume = "144",
doi = "10.1016/j.supflu.2018.10.008"
}

Kuska, R., Milovanović, S., Frerich, S.,& Ivanović, J.. (2019). Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design. in Journal of Supercritical Fluids
Elsevier B.V.., 144, 71-80.
https://doi.org/10.1016/j.supflu.2018.10.008

Kuska R, Milovanović S, Frerich S, Ivanović J. Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design. in Journal of Supercritical Fluids. 2019;144:71-80.
doi:10.1016/j.supflu.2018.10.008 .

Kuska, Robert, Milovanović, Stoja, Frerich, Sulamith, Ivanović, Jasna, "Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design" in Journal of Supercritical Fluids, 144 (2019):71-80,
https://doi.org/10.1016/j.supflu.2018.10.008 . .

TY  - JOUR
AU  - Kuska, Robert
AU  - Milovanović, Stoja
AU  - Frerich, Sulamith
AU  - Ivanović, Jasna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4328
AB  - Thermal properties of extrusion and injection grade polylactic acid (PLA) were analysed using high pressure differential scanning calorimetry (HP-DSC) under CO2 pressures of up to 50 MPa. The greatest depression of melting point and degree of crystallinity of the samples occurred at 20-30 MPa (similar to 97-115 degrees C). Batch and semi continuous processes for supercritical foaming and impregnation of PLA with thymol or thyme extract were performed at 30 MPa and 100-110 degrees C to prevent thymol degradation, decrease heating requirements and ease polymer processing. At these conditions, PLA foams containing 5.6% or 1.1% of thymol and 0.7% of thyme extract were obtained using static or dynamic batch impregnation and semi-continuous extraction-impregnation process for 7 h, respectively. DSC and HP-DSC analyses revealed more pronounced effect of scCO(2) plasticizing than foaming on PLA crystallinity. Neat and impregnated PLA foams with pores size of 15-200 pm have potential for food packaging, biomedical and insulation applications.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design
EP  - 80
SP  - 71
VL  - 144
DO  - 10.1016/j.supflu.2018.10.008
ER  - 

@article{
author = "Kuska, Robert and Milovanović, Stoja and Frerich, Sulamith and Ivanović, Jasna",
year = "2019",
abstract = "Thermal properties of extrusion and injection grade polylactic acid (PLA) were analysed using high pressure differential scanning calorimetry (HP-DSC) under CO2 pressures of up to 50 MPa. The greatest depression of melting point and degree of crystallinity of the samples occurred at 20-30 MPa (similar to 97-115 degrees C). Batch and semi continuous processes for supercritical foaming and impregnation of PLA with thymol or thyme extract were performed at 30 MPa and 100-110 degrees C to prevent thymol degradation, decrease heating requirements and ease polymer processing. At these conditions, PLA foams containing 5.6% or 1.1% of thymol and 0.7% of thyme extract were obtained using static or dynamic batch impregnation and semi-continuous extraction-impregnation process for 7 h, respectively. DSC and HP-DSC analyses revealed more pronounced effect of scCO(2) plasticizing than foaming on PLA crystallinity. Neat and impregnated PLA foams with pores size of 15-200 pm have potential for food packaging, biomedical and insulation applications.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design",
pages = "80-71",
volume = "144",
doi = "10.1016/j.supflu.2018.10.008"
}

Kuska, R., Milovanović, S., Frerich, S.,& Ivanović, J.. (2019). Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 144, 71-80.
https://doi.org/10.1016/j.supflu.2018.10.008

Kuska R, Milovanović S, Frerich S, Ivanović J. Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design. in Journal of Supercritical Fluids. 2019;144:71-80.
doi:10.1016/j.supflu.2018.10.008 .

Kuska, Robert, Milovanović, Stoja, Frerich, Sulamith, Ivanović, Jasna, "Thermal analysis of polylactic acid under high CO2 pressure applied in supercritical impregnation and foaming process design" in Journal of Supercritical Fluids, 144 (2019):71-80,
https://doi.org/10.1016/j.supflu.2018.10.008 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Mraković, Ana
AU  - Kuska, Robert
AU  - Žižović, Irena
AU  - Frerich, Sulamith
AU  - Ivanović, Jasna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4173
AB  - Amorphous, medical grade poly(D,L-lactic acid) (PLA) and poly(D,L-lactic-co-glycolic acid) (PLGA) were used to develop systems for controlled release of a natural bioactive substance - thymol. Supercritical carbon dioxide (scCO(2)) was successfully used both as an impregnation medium for thymol incorporation into the polymer matrix and a foaming agent in a single-step batch process. Impregnation of samples using low to moderate scCO(2) densities (273 kg/m(3) and 630 kg/m(3)) and short processing times (2 h and 4 h) enabled thymol loading of 0.920/0-6.62% and formation of microcellular foams upon system depressurization. Thymol effect on structural and thermal properties on foamed samples was proven by FTIR and DSC. The effect of CO2 under elevated pressure on the neat polymers was analysed by high pressure DSC. Foaming of polymers with lower molecular weight by CO2 of higher density yielded foams with smaller pores. All tested foams released thymol in a controlled manner in phosphate buffered saline (PBS) at 37 degrees C within 3 to 6 weeks. Higher loading and lower cell density favoured thymol release rate, while its concentration in PBS for the tested period depended on foam interaction with the medium. Representative PLGA foam sample with the highest thymol loading (6.62%) showed controlled thymol release within 72 h in mediums having pH values from 1.1 to 7.4.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Science & Engineering C-Materials for Biological Applications
T1  - Supercritical CO2 - assisted production of PLA and PLGA foams for controlled thymol release
EP  - 404
SP  - 394
VL  - 99
DO  - 10.1016/j.msec.2019.01.106
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Mraković, Ana and Kuska, Robert and Žižović, Irena and Frerich, Sulamith and Ivanović, Jasna",
year = "2019",
abstract = "Amorphous, medical grade poly(D,L-lactic acid) (PLA) and poly(D,L-lactic-co-glycolic acid) (PLGA) were used to develop systems for controlled release of a natural bioactive substance - thymol. Supercritical carbon dioxide (scCO(2)) was successfully used both as an impregnation medium for thymol incorporation into the polymer matrix and a foaming agent in a single-step batch process. Impregnation of samples using low to moderate scCO(2) densities (273 kg/m(3) and 630 kg/m(3)) and short processing times (2 h and 4 h) enabled thymol loading of 0.920/0-6.62% and formation of microcellular foams upon system depressurization. Thymol effect on structural and thermal properties on foamed samples was proven by FTIR and DSC. The effect of CO2 under elevated pressure on the neat polymers was analysed by high pressure DSC. Foaming of polymers with lower molecular weight by CO2 of higher density yielded foams with smaller pores. All tested foams released thymol in a controlled manner in phosphate buffered saline (PBS) at 37 degrees C within 3 to 6 weeks. Higher loading and lower cell density favoured thymol release rate, while its concentration in PBS for the tested period depended on foam interaction with the medium. Representative PLGA foam sample with the highest thymol loading (6.62%) showed controlled thymol release within 72 h in mediums having pH values from 1.1 to 7.4.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Science & Engineering C-Materials for Biological Applications",
title = "Supercritical CO2 - assisted production of PLA and PLGA foams for controlled thymol release",
pages = "404-394",
volume = "99",
doi = "10.1016/j.msec.2019.01.106"
}

Milovanović, S., Marković, D., Mraković, A., Kuska, R., Žižović, I., Frerich, S.,& Ivanović, J.. (2019). Supercritical CO2 - assisted production of PLA and PLGA foams for controlled thymol release. in Materials Science & Engineering C-Materials for Biological Applications
Elsevier Science Bv, Amsterdam., 99, 394-404.
https://doi.org/10.1016/j.msec.2019.01.106

Milovanović S, Marković D, Mraković A, Kuska R, Žižović I, Frerich S, Ivanović J. Supercritical CO2 - assisted production of PLA and PLGA foams for controlled thymol release. in Materials Science & Engineering C-Materials for Biological Applications. 2019;99:394-404.
doi:10.1016/j.msec.2019.01.106 .

Milovanović, Stoja, Marković, Darka, Mraković, Ana, Kuska, Robert, Žižović, Irena, Frerich, Sulamith, Ivanović, Jasna, "Supercritical CO2 - assisted production of PLA and PLGA foams for controlled thymol release" in Materials Science & Engineering C-Materials for Biological Applications, 99 (2019):394-404,
https://doi.org/10.1016/j.msec.2019.01.106 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Ivanović, Jasna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4061
AB  - This study explores utilization of biodegradable and biocompatible polymers for controlled release of natural bioactive substance. For that purpose, poly(epsilon-caprolactone) (PCL) beads, cellulose acetate (CA) film, and poly lactic-co-glycolic acid (PLGA) flakes were impregnated with thymol by employing environmentally friendly process of supercritical carbon dioxide (scCO(2)) impregnation. At selected pressure and temperature, prolongation of operating time increased thymol loading. Pure scCO(2) did not affect CA film with average pore diameter of approximately 3 mu m, while it enabled change of PCL beads and PLGA flakes into foams with average pore diameter approximately 175 mu m and 87 mu m, respectively. Additionally to scCO(2), thymol acted as a plasticizer increasing pore size of polymers up to three times. Kinetic of thymol release from selected samples was tested using phosphate buffer saline at 37 degrees C and successfully described with Korsmeyer-Peppas, zero-order, first-order, and Higuchi models. The suggested method of PCL, CA, and PLGA supercritical impregnation led to development of porous, solvent free, added-value materials that release thymol in a controlled manner from 5 h to several days.
PB  - Sage Publications Ltd, London
T2  - Cellular Polymers
T1  - Added-value porous materials for controlled thymol release obtained by supercritical CO2 impregnation process
EP  - 166
IS  - 5-6
SP  - 153
VL  - 38
DO  - 10.1177/0262489319872329
ER  - 

@article{
author = "Milovanović, Stoja and Marković, Darka and Ivanović, Jasna",
year = "2019",
abstract = "This study explores utilization of biodegradable and biocompatible polymers for controlled release of natural bioactive substance. For that purpose, poly(epsilon-caprolactone) (PCL) beads, cellulose acetate (CA) film, and poly lactic-co-glycolic acid (PLGA) flakes were impregnated with thymol by employing environmentally friendly process of supercritical carbon dioxide (scCO(2)) impregnation. At selected pressure and temperature, prolongation of operating time increased thymol loading. Pure scCO(2) did not affect CA film with average pore diameter of approximately 3 mu m, while it enabled change of PCL beads and PLGA flakes into foams with average pore diameter approximately 175 mu m and 87 mu m, respectively. Additionally to scCO(2), thymol acted as a plasticizer increasing pore size of polymers up to three times. Kinetic of thymol release from selected samples was tested using phosphate buffer saline at 37 degrees C and successfully described with Korsmeyer-Peppas, zero-order, first-order, and Higuchi models. The suggested method of PCL, CA, and PLGA supercritical impregnation led to development of porous, solvent free, added-value materials that release thymol in a controlled manner from 5 h to several days.",
publisher = "Sage Publications Ltd, London",
journal = "Cellular Polymers",
title = "Added-value porous materials for controlled thymol release obtained by supercritical CO2 impregnation process",
pages = "166-153",
number = "5-6",
volume = "38",
doi = "10.1177/0262489319872329"
}

Milovanović, S., Marković, D.,& Ivanović, J.. (2019). Added-value porous materials for controlled thymol release obtained by supercritical CO2 impregnation process. in Cellular Polymers
Sage Publications Ltd, London., 38(5-6), 153-166.
https://doi.org/10.1177/0262489319872329

Milovanović S, Marković D, Ivanović J. Added-value porous materials for controlled thymol release obtained by supercritical CO2 impregnation process. in Cellular Polymers. 2019;38(5-6):153-166.
doi:10.1177/0262489319872329 .

Milovanović, Stoja, Marković, Darka, Ivanović, Jasna, "Added-value porous materials for controlled thymol release obtained by supercritical CO2 impregnation process" in Cellular Polymers, 38, no. 5-6 (2019):153-166,
https://doi.org/10.1177/0262489319872329 . .

TY  - JOUR
AU  - Radović, Marko N.
AU  - Adamović, Tijana
AU  - Pavlović, Jelena
AU  - Rusmirović, Jelena
AU  - Tadić, Vanja
AU  - Branković, Zorica
AU  - Ivanović, Jasna
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4232
AB  - Supercritical CO2 impregnation process was used for the first time to fabricate biodegradable gelatin-chitosan (G/Ch) films containing dove oil (CO) for potential use in active food packaging. All the impregnations were carried out at moderately low temperature (40 degrees C). Aiming to maximize CO loading in the films with acceptable morphological, structural and thermal properties, CO2 pressure, impregnation time and G:Ch mass ratio were varied. Gelatin fraction in the films of 25-50 wt. %, scCO(2) pressures of 10-20 MPa and impregnation time of 2-6 h impregnation favoured the CO loading. Processing of the film with equal gelatin to chitosan mass ratio (G/Ch(50:50)) at 10 MPa for 2 h yielded sufficiently high loading (56 mg CO/g(film)) without an adverse effect on morphological properties. The G/Ch(50:50) film was therefore chosen for structural and thermal analyses. ATR-FTIR analysis confirmed successful CO incorporation into the G/Ch(50:50) and its interaction with the film. Plasticizing effect of scCO(2) and CO on the film was evidenced by DSC. Incorporation of 56 mg CO/g(film) into the G/Ch(50:50) didn't affect thermal stability of the film. Beside environmental benefits, supercritical impregnation process enables fast fabrication of G/Ch bio-composite films containing CO, thermally stable to 110 degrees C, which is suitable for most of food packaging applications.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Supercritical co2 impregnation of gelatin-chitosan films with clove essential oil and characterization thereof
EP  - 130
IS  - 2
SP  - 119
VL  - 25
DO  - 10.2298/CICEQ180323025R
ER  - 

@article{
author = "Radović, Marko N. and Adamović, Tijana and Pavlović, Jelena and Rusmirović, Jelena and Tadić, Vanja and Branković, Zorica and Ivanović, Jasna",
year = "2019",
abstract = "Supercritical CO2 impregnation process was used for the first time to fabricate biodegradable gelatin-chitosan (G/Ch) films containing dove oil (CO) for potential use in active food packaging. All the impregnations were carried out at moderately low temperature (40 degrees C). Aiming to maximize CO loading in the films with acceptable morphological, structural and thermal properties, CO2 pressure, impregnation time and G:Ch mass ratio were varied. Gelatin fraction in the films of 25-50 wt. %, scCO(2) pressures of 10-20 MPa and impregnation time of 2-6 h impregnation favoured the CO loading. Processing of the film with equal gelatin to chitosan mass ratio (G/Ch(50:50)) at 10 MPa for 2 h yielded sufficiently high loading (56 mg CO/g(film)) without an adverse effect on morphological properties. The G/Ch(50:50) film was therefore chosen for structural and thermal analyses. ATR-FTIR analysis confirmed successful CO incorporation into the G/Ch(50:50) and its interaction with the film. Plasticizing effect of scCO(2) and CO on the film was evidenced by DSC. Incorporation of 56 mg CO/g(film) into the G/Ch(50:50) didn't affect thermal stability of the film. Beside environmental benefits, supercritical impregnation process enables fast fabrication of G/Ch bio-composite films containing CO, thermally stable to 110 degrees C, which is suitable for most of food packaging applications.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Supercritical co2 impregnation of gelatin-chitosan films with clove essential oil and characterization thereof",
pages = "130-119",
number = "2",
volume = "25",
doi = "10.2298/CICEQ180323025R"
}

Radović, M. N., Adamović, T., Pavlović, J., Rusmirović, J., Tadić, V., Branković, Z.,& Ivanović, J.. (2019). Supercritical co2 impregnation of gelatin-chitosan films with clove essential oil and characterization thereof. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 25(2), 119-130.
https://doi.org/10.2298/CICEQ180323025R

Radović MN, Adamović T, Pavlović J, Rusmirović J, Tadić V, Branković Z, Ivanović J. Supercritical co2 impregnation of gelatin-chitosan films with clove essential oil and characterization thereof. in Chemical Industry & Chemical Engineering Quarterly. 2019;25(2):119-130.
doi:10.2298/CICEQ180323025R .

Radović, Marko N., Adamović, Tijana, Pavlović, Jelena, Rusmirović, Jelena, Tadić, Vanja, Branković, Zorica, Ivanović, Jasna, "Supercritical co2 impregnation of gelatin-chitosan films with clove essential oil and characterization thereof" in Chemical Industry & Chemical Engineering Quarterly, 25, no. 2 (2019):119-130,
https://doi.org/10.2298/CICEQ180323025R . .

TY  - JOUR
AU  - Milovanović, Ivan
AU  - Lajin, Bassam
AU  - Braeuer, Simone
AU  - Steiner, Oliver
AU  - Lisa, Fasshold
AU  - Goessler, Walter
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4334
AB  - Selenium (Se) and sulfur (S) speciation analysis in edible and medicinal Se enriched P. pulmonarius extracts was performed. Mycelium, colonized substrate, and fruiting bodies at different harvesting times were analyzed using ion-pairing reversed-phase chromatography coupled to an ICPMS/MS detector. Extraction efficiencies in en-zymatically digested and aqueous extracts were between 45.3 and 109% for Se, depending on the sample type. Selenomethionine (Se-Met) was found to be the major Se-compound, together with a number of unknown Se-species. Cystine (Cys(2)), methionine (Met), and sulfate were also detected and quantified in all samples. Most of the Se-Met (84.0%) and met (75.8%) were found to be in free form in the fruiting body, in contrast with the mycelium where 53.4% of Se-Met and 80.5% of met is incorporated into proteins.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Simultaneous selenium and sulfur speciation analysis in cultivated Pleurotus pulmonarius mushroom
EP  - 236
SP  - 231
VL  - 279
DO  - 10.1016/j.foodchem.2018.12.009
ER  - 

@article{
author = "Milovanović, Ivan and Lajin, Bassam and Braeuer, Simone and Steiner, Oliver and Lisa, Fasshold and Goessler, Walter",
year = "2019",
abstract = "Selenium (Se) and sulfur (S) speciation analysis in edible and medicinal Se enriched P. pulmonarius extracts was performed. Mycelium, colonized substrate, and fruiting bodies at different harvesting times were analyzed using ion-pairing reversed-phase chromatography coupled to an ICPMS/MS detector. Extraction efficiencies in en-zymatically digested and aqueous extracts were between 45.3 and 109% for Se, depending on the sample type. Selenomethionine (Se-Met) was found to be the major Se-compound, together with a number of unknown Se-species. Cystine (Cys(2)), methionine (Met), and sulfate were also detected and quantified in all samples. Most of the Se-Met (84.0%) and met (75.8%) were found to be in free form in the fruiting body, in contrast with the mycelium where 53.4% of Se-Met and 80.5% of met is incorporated into proteins.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Simultaneous selenium and sulfur speciation analysis in cultivated Pleurotus pulmonarius mushroom",
pages = "236-231",
volume = "279",
doi = "10.1016/j.foodchem.2018.12.009"
}

Milovanović, I., Lajin, B., Braeuer, S., Steiner, O., Lisa, F.,& Goessler, W.. (2019). Simultaneous selenium and sulfur speciation analysis in cultivated Pleurotus pulmonarius mushroom. in Food Chemistry
Elsevier Sci Ltd, Oxford., 279, 231-236.
https://doi.org/10.1016/j.foodchem.2018.12.009

Milovanović I, Lajin B, Braeuer S, Steiner O, Lisa F, Goessler W. Simultaneous selenium and sulfur speciation analysis in cultivated Pleurotus pulmonarius mushroom. in Food Chemistry. 2019;279:231-236.
doi:10.1016/j.foodchem.2018.12.009 .

Milovanović, Ivan, Lajin, Bassam, Braeuer, Simone, Steiner, Oliver, Lisa, Fasshold, Goessler, Walter, "Simultaneous selenium and sulfur speciation analysis in cultivated Pleurotus pulmonarius mushroom" in Food Chemistry, 279 (2019):231-236,
https://doi.org/10.1016/j.foodchem.2018.12.009 . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Senerović, Lidija
AU  - Morić, Ivana
AU  - Adamović, Tijana
AU  - Jovanović, Milena
AU  - Kalagasidis Krušić, Melina
AU  - Mišić, Dušan
AU  - Stojanović, Dušica
AU  - Milovanović, Stoja
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3923
AB  - This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus
EP  - 20
SP  - 11
VL  - 140
DO  - 10.1016/j.supflu.2018.05.025
ER  - 

@article{
author = "Žižović, Irena and Senerović, Lidija and Morić, Ivana and Adamović, Tijana and Jovanović, Milena and Kalagasidis Krušić, Melina and Mišić, Dušan and Stojanović, Dušica and Milovanović, Stoja",
year = "2018",
abstract = "This study discusses utilization of supercritical carbon dioxide for impregnation of cellulose acetate films with thymol in order to produce material with anti-biofilm activity against Pseudomonas aeruginosa and Staphylococcus aureus. Analysis of anti-biofilm activity of cellulose acetate beads impregnated with thymol suggested that optimal thymol loading was in the range from 26% to 30% for efficient reduction of biofilm formation and eradication of pre-formed biofilms. Polymer films were fabricated by the solvent casting method from polymer solutions of different contents, and loaded with thymol using supercritical carbon dioxide at 15.5 MPa and 35 degrees C. The film containing 30% of thymol (F1 30%) exhibited substantial anti-adhesion properties inhibiting biofilm formation on its surface and considerably reduced formation of biofilms on the surrounding surfaces (up to 80%) by all tested strains including antibiotic resistant P. aeruginosa DM50 and methicillin-resistant S. aureus.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus",
pages = "20-11",
volume = "140",
doi = "10.1016/j.supflu.2018.05.025"
}

Žižović, I., Senerović, L., Morić, I., Adamović, T., Jovanović, M., Kalagasidis Krušić, M., Mišić, D., Stojanović, D.,& Milovanović, S.. (2018). Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 140, 11-20.
https://doi.org/10.1016/j.supflu.2018.05.025

Žižović I, Senerović L, Morić I, Adamović T, Jovanović M, Kalagasidis Krušić M, Mišić D, Stojanović D, Milovanović S. Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus. in Journal of Supercritical Fluids. 2018;140:11-20.
doi:10.1016/j.supflu.2018.05.025 .

Žižović, Irena, Senerović, Lidija, Morić, Ivana, Adamović, Tijana, Jovanović, Milena, Kalagasidis Krušić, Melina, Mišić, Dušan, Stojanović, Dušica, Milovanović, Stoja, "Utilization of supercritical carbon dioxide in fabrication of cellulose acetate films with anti-biofilm effects against Pseudomonas aeruginosa and Staphylococcus aureus" in Journal of Supercritical Fluids, 140 (2018):11-20,
https://doi.org/10.1016/j.supflu.2018.05.025 . .

TY  - JOUR
AU  - Terzić, Ivan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
AU  - Lučić-Škorić, Marija
AU  - Milosavljević, Nedeljko
AU  - Milašinović, Nikola
AU  - Kalagasidis Krušić, Melina
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3946
AB  - Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018.
PB  - Wiley, Hoboken
T2  - Polymer Engineering and Science
T1  - A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol
EP  - 2199
IS  - 12
SP  - 2192
VL  - 58
DO  - 10.1002/pen.24834
ER  - 

@article{
author = "Terzić, Ivan and Ivanović, Jasna and Žižović, Irena and Lučić-Škorić, Marija and Milosavljević, Nedeljko and Milašinović, Nikola and Kalagasidis Krušić, Melina",
year = "2018",
abstract = "Supercritical carbon dioxide (scCO(2)) technology was used for preparation of functional pH sensitive chitosan-based aerogels characterized with micron size pores and their impregnation with thymol as a natural bioactive substance. Hydrogels based on chitosan, itaconic and methacrylic acids were transformed to alcogels and dried in the air or with scCO(2) to obtain xero- and aerogels, respectively. Applying 10 min of static and 120 min of dynamic scCO(2) drying at 11 MPa and 45 degrees C followed with the decompression at a rate of 1 MPa/min yielded an advantageous aerogel with favorable swelling kinetics and elasticity, compared to the xerogel and aerogels obtained at other decompression rates and drying times. This aerogel was successfully loaded with thymol (up to 4.6 wt.%) using supercritical scCO(2) at 10 MPa and 35 degrees C. In vitro studies of swelling in PBS at 37 degrees C indicated a great potential of the obtained stimuli-responsive chitosan gels for topical administration of thymol known for antimicrobial, antioxidant and anti-inflammatory activities. POLYM. ENG. SCI., 58:2192-2199, 2018.",
publisher = "Wiley, Hoboken",
journal = "Polymer Engineering and Science",
title = "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol",
pages = "2199-2192",
number = "12",
volume = "58",
doi = "10.1002/pen.24834"
}

Terzić, I., Ivanović, J., Žižović, I., Lučić-Škorić, M., Milosavljević, N., Milašinović, N.,& Kalagasidis Krušić, M.. (2018). A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer Engineering and Science
Wiley, Hoboken., 58(12), 2192-2199.
https://doi.org/10.1002/pen.24834

Terzić I, Ivanović J, Žižović I, Lučić-Škorić M, Milosavljević N, Milašinović N, Kalagasidis Krušić M. A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol. in Polymer Engineering and Science. 2018;58(12):2192-2199.
doi:10.1002/pen.24834 .

Terzić, Ivan, Ivanović, Jasna, Žižović, Irena, Lučić-Škorić, Marija, Milosavljević, Nedeljko, Milašinović, Nikola, Kalagasidis Krušić, Melina, "A novel chitosan gels: Supercritical CO2 drying and impregnation with thymol" in Polymer Engineering and Science, 58, no. 12 (2018):2192-2199,
https://doi.org/10.1002/pen.24834 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Radetić, Maja
AU  - Stojanović, Dušica
AU  - Janković-Častvan, Ivona
AU  - Tadić, Vanja
AU  - Stanković, Miroslav
AU  - Jovanović, Dušan M.
AU  - Žižović, Irena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4035
AB  - This study discusses the possibility of supercritical solvent impregnation of polypropylene and polyamide fabrics as well as cellulose acetate in the form of beads and films with pyrethrum extract in order to fabricate functionalized materials with repellent properties. Impregnation experiments were performed at temperature of 40 degrees C and pressures of 10 and 20 MPa in supercritical carbon dioxide. The time of impregnation was varied from 0.5 to 18 h. Loadings of pyrethrum extract into polypropylene fabrics and cellulose acetate films higher than 30% were attainable at 10 MPa. In the case of polyamide fabrics and cellulose acetate beads, the pressure of 20 MPa was needed for obtaining the loadings higher than 11%. Pyrethrum extract release study revealed different capabilities of the tested materials for the repellent release, implying a variety of their possible applications. The impregnated solids were characterized by SEM, DSC, FTIR, Mercury intrusion porosimetry analyses and HPLC analyses.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Functionalization of polypropylene, polyamide and cellulose acetate materials with pyrethrum extract as a natural repellent in supercritical carbon dioxide
EP  - 81
SP  - 70
VL  - 136
DO  - 10.1016/j.supflu.2018.02.014
ER  - 

@article{
author = "Pajnik, Jelena and Radetić, Maja and Stojanović, Dušica and Janković-Častvan, Ivona and Tadić, Vanja and Stanković, Miroslav and Jovanović, Dušan M. and Žižović, Irena",
year = "2018",
abstract = "This study discusses the possibility of supercritical solvent impregnation of polypropylene and polyamide fabrics as well as cellulose acetate in the form of beads and films with pyrethrum extract in order to fabricate functionalized materials with repellent properties. Impregnation experiments were performed at temperature of 40 degrees C and pressures of 10 and 20 MPa in supercritical carbon dioxide. The time of impregnation was varied from 0.5 to 18 h. Loadings of pyrethrum extract into polypropylene fabrics and cellulose acetate films higher than 30% were attainable at 10 MPa. In the case of polyamide fabrics and cellulose acetate beads, the pressure of 20 MPa was needed for obtaining the loadings higher than 11%. Pyrethrum extract release study revealed different capabilities of the tested materials for the repellent release, implying a variety of their possible applications. The impregnated solids were characterized by SEM, DSC, FTIR, Mercury intrusion porosimetry analyses and HPLC analyses.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Functionalization of polypropylene, polyamide and cellulose acetate materials with pyrethrum extract as a natural repellent in supercritical carbon dioxide",
pages = "81-70",
volume = "136",
doi = "10.1016/j.supflu.2018.02.014"
}

Pajnik, J., Radetić, M., Stojanović, D., Janković-Častvan, I., Tadić, V., Stanković, M., Jovanović, D. M.,& Žižović, I.. (2018). Functionalization of polypropylene, polyamide and cellulose acetate materials with pyrethrum extract as a natural repellent in supercritical carbon dioxide. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 136, 70-81.
https://doi.org/10.1016/j.supflu.2018.02.014

Pajnik J, Radetić M, Stojanović D, Janković-Častvan I, Tadić V, Stanković M, Jovanović DM, Žižović I. Functionalization of polypropylene, polyamide and cellulose acetate materials with pyrethrum extract as a natural repellent in supercritical carbon dioxide. in Journal of Supercritical Fluids. 2018;136:70-81.
doi:10.1016/j.supflu.2018.02.014 .

Pajnik, Jelena, Radetić, Maja, Stojanović, Dušica, Janković-Častvan, Ivona, Tadić, Vanja, Stanković, Miroslav, Jovanović, Dušan M., Žižović, Irena, "Functionalization of polypropylene, polyamide and cellulose acetate materials with pyrethrum extract as a natural repellent in supercritical carbon dioxide" in Journal of Supercritical Fluids, 136 (2018):70-81,
https://doi.org/10.1016/j.supflu.2018.02.014 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Hollermann, Gesa
AU  - Errenst, Cornelia
AU  - Pajnik, Jelena
AU  - Frerich, Sulamith
AU  - Kroll, Stephen
AU  - Rezwan, Kurosch
AU  - Ivanović, Jasna
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4037
AB  - Biodegradable polymers with antibacterial properties are highly desirable materials for active food packaging applications. Thymol, a dietary monoterpene phenol with a strong antibacterial activity is abundant in plants belonging to the genus Thymus. This study presents two approaches for supercritical CO2 impregnation of poly (lactic acid)(PLA)/poly(epsilon-caprolactone)(PCL) blended films to induce antibacterial properties of the material: (i) a batch impregnation process for loading pure thymol, and (ii) an integrated supercritical extraction-impregnation process for isolation of thyme extract and its incorporation into the films, operated in both batch or semi-continuous modes with supercritical solution circulation. The PCL content in films, impregnation time and CO2 flow regime were varied to maximize loading of the films with thymol or thyme extract with preserving films' structure and thermal stability. Representative film samples impregnated with thymol and thyme extract were tested against Gram ( - ) (Escherichia col) and Gram( + ) (Bacillus subtilis) model strains, by measuring their metabolic activity and re-cultivation after exposure to the films. The film containing thymol (35.8 wt%) showed a strong antibacterial activity leading to a total reduction of bacterial cell viability. Proposed processes enable fast, controlled and organic solvent-free fabrication of the PLA/PCL films containing natural antibacterial substances at moderately low temperature, with a compact structure and a good thermal stability, for potential use as active food packaging materials.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Supercritical CO2 impregnation of PLA/PCL films with natural substances for bacterial growth control in food packaging
EP  - 495
SP  - 486
VL  - 107
DO  - 10.1016/j.foodres.2018.02.065
ER  - 

@article{
author = "Milovanović, Stoja and Hollermann, Gesa and Errenst, Cornelia and Pajnik, Jelena and Frerich, Sulamith and Kroll, Stephen and Rezwan, Kurosch and Ivanović, Jasna",
year = "2018",
abstract = "Biodegradable polymers with antibacterial properties are highly desirable materials for active food packaging applications. Thymol, a dietary monoterpene phenol with a strong antibacterial activity is abundant in plants belonging to the genus Thymus. This study presents two approaches for supercritical CO2 impregnation of poly (lactic acid)(PLA)/poly(epsilon-caprolactone)(PCL) blended films to induce antibacterial properties of the material: (i) a batch impregnation process for loading pure thymol, and (ii) an integrated supercritical extraction-impregnation process for isolation of thyme extract and its incorporation into the films, operated in both batch or semi-continuous modes with supercritical solution circulation. The PCL content in films, impregnation time and CO2 flow regime were varied to maximize loading of the films with thymol or thyme extract with preserving films' structure and thermal stability. Representative film samples impregnated with thymol and thyme extract were tested against Gram ( - ) (Escherichia col) and Gram( + ) (Bacillus subtilis) model strains, by measuring their metabolic activity and re-cultivation after exposure to the films. The film containing thymol (35.8 wt%) showed a strong antibacterial activity leading to a total reduction of bacterial cell viability. Proposed processes enable fast, controlled and organic solvent-free fabrication of the PLA/PCL films containing natural antibacterial substances at moderately low temperature, with a compact structure and a good thermal stability, for potential use as active food packaging materials.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Supercritical CO2 impregnation of PLA/PCL films with natural substances for bacterial growth control in food packaging",
pages = "495-486",
volume = "107",
doi = "10.1016/j.foodres.2018.02.065"
}

Milovanović, S., Hollermann, G., Errenst, C., Pajnik, J., Frerich, S., Kroll, S., Rezwan, K.,& Ivanović, J.. (2018). Supercritical CO2 impregnation of PLA/PCL films with natural substances for bacterial growth control in food packaging. in Food Research International
Elsevier Science Bv, Amsterdam., 107, 486-495.
https://doi.org/10.1016/j.foodres.2018.02.065

Milovanović S, Hollermann G, Errenst C, Pajnik J, Frerich S, Kroll S, Rezwan K, Ivanović J. Supercritical CO2 impregnation of PLA/PCL films with natural substances for bacterial growth control in food packaging. in Food Research International. 2018;107:486-495.
doi:10.1016/j.foodres.2018.02.065 .

Milovanović, Stoja, Hollermann, Gesa, Errenst, Cornelia, Pajnik, Jelena, Frerich, Sulamith, Kroll, Stephen, Rezwan, Kurosch, Ivanović, Jasna, "Supercritical CO2 impregnation of PLA/PCL films with natural substances for bacterial growth control in food packaging" in Food Research International, 107 (2018):486-495,
https://doi.org/10.1016/j.foodres.2018.02.065 . .

TY  - THES
AU  - Pajnik, Jelena
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6556
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:19318/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50897423
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4676
AB  - This PhD thesis was aimed to investigate the possibility of supercritical solvent impregnation (SSI) of selected polymer materials (cotton fabric, polypropylene (PP) non-woven fabric, polyamide (PA) polymers (fabric, nanofibres) and polymers of cellulose acetate (CA) in the form of granules and films) with pyrethrin extract in order to develop functionalized materials with repellant properties. Pyrethrum extract obtained from Chrysanthemum cinerariaefolium with proven insecticidal properities was selected as an active substance in the impregnation experiments. Determination of the pyrethrum extract solubility in supercritical carbon dioxide (scCO2) and the SSI of selected solids with the same extract were studied in the high pressure view cell (25 cm3) and the high pressure laboratory system (300 cm3) using a static mode. The pyrethrum extract release from the impregnated substrates was evaluated by exposing them to air for 30 days under controlled temperature (25 °C) with an use of digital incubator. Impregnated substrates were analized by FT-IR, FE-SEM, DSC, HPLC and mercury intrusion porosimetry methods. The solubilities of pyrethrum extract in scCO2 were in the range of 15.09-48.69 kg/m3. Impregnated cotton fabrics with pyrethrum contents of 0.5% and 1% exhibited significant repellent activity against ticks. The SSI enabled incorporation of large amounts of the extract into the PP non-woven fabric (up to 78.01%). Impregnation yields of PA and CA polymers were in the range of 4.55-59.59% and 0.86-30.43%, respectively. The impregnated PP non-woven fabrics revieled potential to release large amounts of repellent ( gt 70% for 30 days period), while repellent content in the impregnated PA substrates remined stable after 30 days of exposure to air.
AB  - U ovoj doktorskoj disertaciji su ispitane mogućnosti natkritične impregnacije (NKI) različitih polimernih nosača (pamučna tkanina, polipropilenski (PP) netkani materijal, poliamidni (PA) tekstilni materijali (tkanina, nanovlakna) i acetat celuloze (AC) u formi granula i filmova) ekstraktom piretrina, kako bi se istražila mogućnost dobijanja funkcionalizovanih materijala sa repelentnim svojstvima. Kao aktivna supstanca u procesima impregnacije je korišćen ekstrakt piretrina iz cveta Chrysanthemum cinerariaefolium zbog svog dokazanog insekticidnog dejstva. Rastvorljivost ekstrakta piretrina u natkritičnom ugljenik(IV)-oksidu (nkCO2) i mogućnost NKI odabranih polimera ekstraktom, ispitane su korišćenjem sledeće opreme: ćelija za rad pod visokim pritiscima (25 cm3) i laboratorijsko postrojenje za rad pod visokim pritiscima (300 cm3), primenom statičke metode. Otpuštanje ekstrakta iz impregniranih supstrata je ispitivano na vazduhu tokom 30 dana u kontrolisanim uslovima temperature (25 °C) u digitalnom inkubatoru. Impregnirani nosači su analizirani sledećim metodama: FT-IR, FE-SEM, DSC, HPLC i metodom živine porozimetrije. Vrednosti rastvorljivosti ekstrakta piretrina u nkCO2 bile su u opsegu od 15,09-48,69 kg/m3. Sadržaji impregniranog ekstrakta na pamučnoj tkanini od 0,5% i 1% obezbedili su repelentnu aktivnost materijala protiv krpelja. Impregnacija PP netkanog materijala je omogućila inkorporaciju velikih količina ekstrakta piretrina u polimer (do 78,01%), dok su prinosi impregnacije polimera na bazi PA bili u opsegu od 4,55-59,59%, a za AC od 0,86-30,43%. Impregnirana PP vlakna su pokazala sposobnost da otpuste velike količine ekstrakta ( gt 70% tokom 30 dana), dok je sadržaj impregniranog ekstrakta u PA polimerima ostao stabilan nakon ispitivanog perioda.
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Application of supercritical carbon dioxide in fabrication of materials with repellent properties based on pyrethrins
T1  - Primena natkritičnog ugljenik(IV)-oksida za dobijanje materijala sa repelentnim svojstvima na bazi piretrina
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4676
ER  - 

@phdthesis{
author = "Pajnik, Jelena",
year = "2018",
abstract = "This PhD thesis was aimed to investigate the possibility of supercritical solvent impregnation (SSI) of selected polymer materials (cotton fabric, polypropylene (PP) non-woven fabric, polyamide (PA) polymers (fabric, nanofibres) and polymers of cellulose acetate (CA) in the form of granules and films) with pyrethrin extract in order to develop functionalized materials with repellant properties. Pyrethrum extract obtained from Chrysanthemum cinerariaefolium with proven insecticidal properities was selected as an active substance in the impregnation experiments. Determination of the pyrethrum extract solubility in supercritical carbon dioxide (scCO2) and the SSI of selected solids with the same extract were studied in the high pressure view cell (25 cm3) and the high pressure laboratory system (300 cm3) using a static mode. The pyrethrum extract release from the impregnated substrates was evaluated by exposing them to air for 30 days under controlled temperature (25 °C) with an use of digital incubator. Impregnated substrates were analized by FT-IR, FE-SEM, DSC, HPLC and mercury intrusion porosimetry methods. The solubilities of pyrethrum extract in scCO2 were in the range of 15.09-48.69 kg/m3. Impregnated cotton fabrics with pyrethrum contents of 0.5% and 1% exhibited significant repellent activity against ticks. The SSI enabled incorporation of large amounts of the extract into the PP non-woven fabric (up to 78.01%). Impregnation yields of PA and CA polymers were in the range of 4.55-59.59% and 0.86-30.43%, respectively. The impregnated PP non-woven fabrics revieled potential to release large amounts of repellent ( gt 70% for 30 days period), while repellent content in the impregnated PA substrates remined stable after 30 days of exposure to air., U ovoj doktorskoj disertaciji su ispitane mogućnosti natkritične impregnacije (NKI) različitih polimernih nosača (pamučna tkanina, polipropilenski (PP) netkani materijal, poliamidni (PA) tekstilni materijali (tkanina, nanovlakna) i acetat celuloze (AC) u formi granula i filmova) ekstraktom piretrina, kako bi se istražila mogućnost dobijanja funkcionalizovanih materijala sa repelentnim svojstvima. Kao aktivna supstanca u procesima impregnacije je korišćen ekstrakt piretrina iz cveta Chrysanthemum cinerariaefolium zbog svog dokazanog insekticidnog dejstva. Rastvorljivost ekstrakta piretrina u natkritičnom ugljenik(IV)-oksidu (nkCO2) i mogućnost NKI odabranih polimera ekstraktom, ispitane su korišćenjem sledeće opreme: ćelija za rad pod visokim pritiscima (25 cm3) i laboratorijsko postrojenje za rad pod visokim pritiscima (300 cm3), primenom statičke metode. Otpuštanje ekstrakta iz impregniranih supstrata je ispitivano na vazduhu tokom 30 dana u kontrolisanim uslovima temperature (25 °C) u digitalnom inkubatoru. Impregnirani nosači su analizirani sledećim metodama: FT-IR, FE-SEM, DSC, HPLC i metodom živine porozimetrije. Vrednosti rastvorljivosti ekstrakta piretrina u nkCO2 bile su u opsegu od 15,09-48,69 kg/m3. Sadržaji impregniranog ekstrakta na pamučnoj tkanini od 0,5% i 1% obezbedili su repelentnu aktivnost materijala protiv krpelja. Impregnacija PP netkanog materijala je omogućila inkorporaciju velikih količina ekstrakta piretrina u polimer (do 78,01%), dok su prinosi impregnacije polimera na bazi PA bili u opsegu od 4,55-59,59%, a za AC od 0,86-30,43%. Impregnirana PP vlakna su pokazala sposobnost da otpuste velike količine ekstrakta ( gt 70% tokom 30 dana), dok je sadržaj impregniranog ekstrakta u PA polimerima ostao stabilan nakon ispitivanog perioda.",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Application of supercritical carbon dioxide in fabrication of materials with repellent properties based on pyrethrins, Primena natkritičnog ugljenik(IV)-oksida za dobijanje materijala sa repelentnim svojstvima na bazi piretrina",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4676"
}

Pajnik, J.. (2018). Application of supercritical carbon dioxide in fabrication of materials with repellent properties based on pyrethrins. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_technorep_4676

Pajnik J. Application of supercritical carbon dioxide in fabrication of materials with repellent properties based on pyrethrins. 2018;.
https://hdl.handle.net/21.15107/rcub_technorep_4676 .

Pajnik, Jelena, "Application of supercritical carbon dioxide in fabrication of materials with repellent properties based on pyrethrins" (2018),
https://hdl.handle.net/21.15107/rcub_technorep_4676 .

TY  - JOUR
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
AU  - Ivanović, Jasna
AU  - Radmanović, Tanja
AU  - Milovanović, Stoja
AU  - Stanković, Milica
AU  - Žižović, Irena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3867
AB  - Supercritical CO2 extraction of Helichrysum italicum and impregnation of starch xerogels with the extract by using an integrated scCO(2) extraction and impregnation process were performed at 350 bar and 40 degrees C in order to produce biomaterials for possible oral intake of the extract. Xerogels produced by air-drying of acetogels and alcogels were used as carriers in the supercritical impregnation process. The effect of ethanol as a co-solvent, contact time, plant material/carrier mass ratio and xerogel preparation on the impregnation loading was studied. The highest impregnation loading (1.261 +/- 0.22%) was achieved after 5 h impregnation of the xerogel obtained from alcogel using pure scCO(2) and plant material/carrier mass ratio of 10. Chemical analysis of the extracts showed that the addition of ethanol as co-solvent had a positive effect on scCO(2) selectivity to terpene fraction and total flavonoids, while it lowered the total phenolic content. Despite the difference in chemical composition, both extracts expressed similar antioxidant activity according to the DPPH and FRAP methods. The integrated process was shown to be a feasible method for isolation and incorporation of bioactive components of H italicum into starch xerogels.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel
EP  - 200
IS  - 2
SP  - 191
VL  - 24
DO  - 10.2298/CICEQ170223031M
ER  - 

@article{
author = "Maksimović, Svetolik and Tadić, Vanja and Ivanović, Jasna and Radmanović, Tanja and Milovanović, Stoja and Stanković, Milica and Žižović, Irena",
year = "2018",
abstract = "Supercritical CO2 extraction of Helichrysum italicum and impregnation of starch xerogels with the extract by using an integrated scCO(2) extraction and impregnation process were performed at 350 bar and 40 degrees C in order to produce biomaterials for possible oral intake of the extract. Xerogels produced by air-drying of acetogels and alcogels were used as carriers in the supercritical impregnation process. The effect of ethanol as a co-solvent, contact time, plant material/carrier mass ratio and xerogel preparation on the impregnation loading was studied. The highest impregnation loading (1.261 +/- 0.22%) was achieved after 5 h impregnation of the xerogel obtained from alcogel using pure scCO(2) and plant material/carrier mass ratio of 10. Chemical analysis of the extracts showed that the addition of ethanol as co-solvent had a positive effect on scCO(2) selectivity to terpene fraction and total flavonoids, while it lowered the total phenolic content. Despite the difference in chemical composition, both extracts expressed similar antioxidant activity according to the DPPH and FRAP methods. The integrated process was shown to be a feasible method for isolation and incorporation of bioactive components of H italicum into starch xerogels.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel",
pages = "200-191",
number = "2",
volume = "24",
doi = "10.2298/CICEQ170223031M"
}

Maksimović, S., Tadić, V., Ivanović, J., Radmanović, T., Milovanović, S., Stanković, M.,& Žižović, I.. (2018). Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 24(2), 191-200.
https://doi.org/10.2298/CICEQ170223031M

Maksimović S, Tadić V, Ivanović J, Radmanović T, Milovanović S, Stanković M, Žižović I. Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel. in Chemical Industry & Chemical Engineering Quarterly. 2018;24(2):191-200.
doi:10.2298/CICEQ170223031M .

Maksimović, Svetolik, Tadić, Vanja, Ivanović, Jasna, Radmanović, Tanja, Milovanović, Stoja, Stanković, Milica, Žižović, Irena, "Utilization of the integrated process of supercritical extraction and impregnation for incorporation of helichrysum italicum extract into corn starch xerogel" in Chemical Industry & Chemical Engineering Quarterly, 24, no. 2 (2018):191-200,
https://doi.org/10.2298/CICEQ170223031M . .

TY  - JOUR
AU  - Stanković, Milica
AU  - Maksimović, Svetolik
AU  - Tadić, Vanja
AU  - Arsić, Ivana A.
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3811
AB  - Oils from wild fruits have gained attention due to the presence of the wide variety of natural bioactive lipid components (essential polyunsaturated fatty acids, phytosterols, lipid-soluble vitamins), which have positive effects on human health. The present study describes the extraction of oils from fruits of different plant species, Sambucus nigra, Prunus spinosa, Sorbus aucuparia and Rosa canina, using the conventional Soxhlet extraction technique with petroleum ether. Before the extraction, mechanical processing of fruit samples (exposure to elevated pressure and temperature) was performed. The yield of oil (%) was expressed as mass of obtained oil per mass of dry plant material (w/w). Moisture content of fruit samples was also determined. Wild fruits showed different moisture contents, ranging from 6.53 to 71.52 g/100 g of fresh weight, in R. canina and S. aucuparia fruits, respectively. S. nigra and P. spinosa fruits revealed similar moisture contents, 68.48 g/100 g and 68.89 g/100 g of fresh weight, respectively. Oil yields of the investigated fruit samples showed statistically significant differences (p  lt  0.05) and ranged from 0.38 to 3.22% (w/w) in the R. canina and P. spinosa fruits, respectively. Dry fruits of S. nigra had 2.55% (w/w) of oil, while the oil content in S. aucuparia fruits was 0.68% (w/w). The findings obtained from this study might be useful from the aspect of potential utilization of these wild fruits, in particular fruits of P. spinosa and S. nigra, as raw materials for the extraction of valuable oil for the application in food, cosmetic and pharmaceutical industry.
AB  - Ulja dobijena iz plodova biljaka sve više dobijaju na značaju, upravo zbog prisustva velikog broja prirodnih, bioaktivnih lipidnih komponenata (esencijalnih, polinezasićenih masnih kiselina, fitosterola, liposolubilnih vitamina), koji ispoljavaju pozitivne efekte na ljudsko zdravlje. U ovom radu opisan je postupak ekstrakcije ulja iz plodova različitih biljnih vrsta (Sambucus nigra, Prunus spinosa, Sorbus aucuparia i Rosa canina), primenom konvencionalne Soxhlet ekstrakcione tehnike sa petrol-etrom. Pre ekstrakcije izvršena je mehanička obrada uzoraka plodova, njihovim izlaganjem povišenom pritisku i temperaturi. Prinos ulja (%) je izražen kao masa dobijenog ulja u odnosu na masu suvog biljnog materijala (w/w). Određen je i sadržaj vlage u uzorcima plodova. Divlji plodovi su se razlikovali po sadržaju vlage, čije su vrednosti bile u granicama od 6,53 g/100 g svežih plodova (R. canina) do 71,52 g/100 g svežih plodova (S. aucuparia). Biljne vrste S. nigra i P. spinosa pokazale su sličan sadržaj vlage u plodovima, i to 68,48 g/100 g i 68,89 g/100 g svežih plodova. Prinosi ulja ispitivanih uzoraka plodova razlikovali su se statistički značajno (p  lt  0,05), a vrednosti su bile od 0,38% (kod R. canina plodova) do 3,22% (plodovi P. spinosa). Kod suvih plodova S. nigra dobijeni prinos ulja je iznosio 2,55%, dok je sadržaj ulja u plodovima S. aucuparia iznosio 0,68%. Dobijeni rezultati su korisni zbog mogućnosti upotrebe ispitivanih divljih plodova, naročito plodova P. spinosa i S. nigra (imale su najveći sadržaj ulja), kao polaznih sirovina za ekstrakciju biljnog ulja za primenu u prehrambenoj, kozmetičkoj i farmaceutskoj industriji.
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique
T1  - Sadržaj ulja divljih plodova različitih biljnih vrsta dobijen konvencionalnom Soxhlet tehnikom ekstrakcije
EP  - 200
IS  - 3
SP  - 193
VL  - 35
DO  - 10.2478/afmnai-2018-0021
ER  - 

@article{
author = "Stanković, Milica and Maksimović, Svetolik and Tadić, Vanja and Arsić, Ivana A.",
year = "2018",
abstract = "Oils from wild fruits have gained attention due to the presence of the wide variety of natural bioactive lipid components (essential polyunsaturated fatty acids, phytosterols, lipid-soluble vitamins), which have positive effects on human health. The present study describes the extraction of oils from fruits of different plant species, Sambucus nigra, Prunus spinosa, Sorbus aucuparia and Rosa canina, using the conventional Soxhlet extraction technique with petroleum ether. Before the extraction, mechanical processing of fruit samples (exposure to elevated pressure and temperature) was performed. The yield of oil (%) was expressed as mass of obtained oil per mass of dry plant material (w/w). Moisture content of fruit samples was also determined. Wild fruits showed different moisture contents, ranging from 6.53 to 71.52 g/100 g of fresh weight, in R. canina and S. aucuparia fruits, respectively. S. nigra and P. spinosa fruits revealed similar moisture contents, 68.48 g/100 g and 68.89 g/100 g of fresh weight, respectively. Oil yields of the investigated fruit samples showed statistically significant differences (p  lt  0.05) and ranged from 0.38 to 3.22% (w/w) in the R. canina and P. spinosa fruits, respectively. Dry fruits of S. nigra had 2.55% (w/w) of oil, while the oil content in S. aucuparia fruits was 0.68% (w/w). The findings obtained from this study might be useful from the aspect of potential utilization of these wild fruits, in particular fruits of P. spinosa and S. nigra, as raw materials for the extraction of valuable oil for the application in food, cosmetic and pharmaceutical industry., Ulja dobijena iz plodova biljaka sve više dobijaju na značaju, upravo zbog prisustva velikog broja prirodnih, bioaktivnih lipidnih komponenata (esencijalnih, polinezasićenih masnih kiselina, fitosterola, liposolubilnih vitamina), koji ispoljavaju pozitivne efekte na ljudsko zdravlje. U ovom radu opisan je postupak ekstrakcije ulja iz plodova različitih biljnih vrsta (Sambucus nigra, Prunus spinosa, Sorbus aucuparia i Rosa canina), primenom konvencionalne Soxhlet ekstrakcione tehnike sa petrol-etrom. Pre ekstrakcije izvršena je mehanička obrada uzoraka plodova, njihovim izlaganjem povišenom pritisku i temperaturi. Prinos ulja (%) je izražen kao masa dobijenog ulja u odnosu na masu suvog biljnog materijala (w/w). Određen je i sadržaj vlage u uzorcima plodova. Divlji plodovi su se razlikovali po sadržaju vlage, čije su vrednosti bile u granicama od 6,53 g/100 g svežih plodova (R. canina) do 71,52 g/100 g svežih plodova (S. aucuparia). Biljne vrste S. nigra i P. spinosa pokazale su sličan sadržaj vlage u plodovima, i to 68,48 g/100 g i 68,89 g/100 g svežih plodova. Prinosi ulja ispitivanih uzoraka plodova razlikovali su se statistički značajno (p  lt  0,05), a vrednosti su bile od 0,38% (kod R. canina plodova) do 3,22% (plodovi P. spinosa). Kod suvih plodova S. nigra dobijeni prinos ulja je iznosio 2,55%, dok je sadržaj ulja u plodovima S. aucuparia iznosio 0,68%. Dobijeni rezultati su korisni zbog mogućnosti upotrebe ispitivanih divljih plodova, naročito plodova P. spinosa i S. nigra (imale su najveći sadržaj ulja), kao polaznih sirovina za ekstrakciju biljnog ulja za primenu u prehrambenoj, kozmetičkoj i farmaceutskoj industriji.",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique, Sadržaj ulja divljih plodova različitih biljnih vrsta dobijen konvencionalnom Soxhlet tehnikom ekstrakcije",
pages = "200-193",
number = "3",
volume = "35",
doi = "10.2478/afmnai-2018-0021"
}

Stanković, M., Maksimović, S., Tadić, V.,& Arsić, I. A.. (2018). The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique. in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 35(3), 193-200.
https://doi.org/10.2478/afmnai-2018-0021

Stanković M, Maksimović S, Tadić V, Arsić IA. The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique. in Acta Facultatis Medicae Naissensis. 2018;35(3):193-200.
doi:10.2478/afmnai-2018-0021 .

Stanković, Milica, Maksimović, Svetolik, Tadić, Vanja, Arsić, Ivana A., "The oil content of wild fruits from different plant species obtained by conventional Soxhlet extraction technique" in Acta Facultatis Medicae Naissensis, 35, no. 3 (2018):193-200,
https://doi.org/10.2478/afmnai-2018-0021 . .

TY  - JOUR
AU  - Marković, Darka
AU  - Milovanović, Stoja
AU  - De Clerck, Karen
AU  - Žižović, Irena
AU  - Stojanović, Dušica
AU  - Radetić, Maja
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3889
AB  - This study discusses the potential of high pressure (supercritical and liquid) CO2 impregnation to load electrospun polyamide nanofibers with thymol as an antimicrobial agent. The influence of selected pressures and temperatures on the thymol impregnation yield has been evaluated. High pressure impregnation provided superior thymol impregnation yields (up to similar to 60%) on nanofibers compared to conventional immersion methods (similar to 2%). The presence of thymol in impregnated samples was confirmed by FT-IR analysis. FESEM analysis revealed that impregnation with thymol resulted in a swelling of the nanofibers. The nanofiber morphological changes strongly depended on the thymol impregnation yield. Nanofibers impregnated with thymol using high pressure CO2 ensured excellent antimicrobial activity against Gram-negative bacterium E. coli, Gram-positive bacterium S. aureus and fungus C. albicans. In addition, the Higuchi release model well fitted the thymol release studies.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of CO2 Utilization
T1  - Development of material with strong antimicrobial activity by high pressure CO2 impregnation of polyamide nanofibers with thymol
EP  - 27
SP  - 19
VL  - 26
DO  - 10.1016/j.jcou.2018.04.019
ER  - 

@article{
author = "Marković, Darka and Milovanović, Stoja and De Clerck, Karen and Žižović, Irena and Stojanović, Dušica and Radetić, Maja",
year = "2018",
abstract = "This study discusses the potential of high pressure (supercritical and liquid) CO2 impregnation to load electrospun polyamide nanofibers with thymol as an antimicrobial agent. The influence of selected pressures and temperatures on the thymol impregnation yield has been evaluated. High pressure impregnation provided superior thymol impregnation yields (up to similar to 60%) on nanofibers compared to conventional immersion methods (similar to 2%). The presence of thymol in impregnated samples was confirmed by FT-IR analysis. FESEM analysis revealed that impregnation with thymol resulted in a swelling of the nanofibers. The nanofiber morphological changes strongly depended on the thymol impregnation yield. Nanofibers impregnated with thymol using high pressure CO2 ensured excellent antimicrobial activity against Gram-negative bacterium E. coli, Gram-positive bacterium S. aureus and fungus C. albicans. In addition, the Higuchi release model well fitted the thymol release studies.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of CO2 Utilization",
title = "Development of material with strong antimicrobial activity by high pressure CO2 impregnation of polyamide nanofibers with thymol",
pages = "27-19",
volume = "26",
doi = "10.1016/j.jcou.2018.04.019"
}

Marković, D., Milovanović, S., De Clerck, K., Žižović, I., Stojanović, D.,& Radetić, M.. (2018). Development of material with strong antimicrobial activity by high pressure CO2 impregnation of polyamide nanofibers with thymol. in Journal of CO2 Utilization
Elsevier Sci Ltd, Oxford., 26, 19-27.
https://doi.org/10.1016/j.jcou.2018.04.019

Marković D, Milovanović S, De Clerck K, Žižović I, Stojanović D, Radetić M. Development of material with strong antimicrobial activity by high pressure CO2 impregnation of polyamide nanofibers with thymol. in Journal of CO2 Utilization. 2018;26:19-27.
doi:10.1016/j.jcou.2018.04.019 .

Marković, Darka, Milovanović, Stoja, De Clerck, Karen, Žižović, Irena, Stojanović, Dušica, Radetić, Maja, "Development of material with strong antimicrobial activity by high pressure CO2 impregnation of polyamide nanofibers with thymol" in Journal of CO2 Utilization, 26 (2018):19-27,
https://doi.org/10.1016/j.jcou.2018.04.019 . .

TY  - JOUR
AU  - Adamović, Tijana
AU  - Milovanović, Stoja
AU  - Marković, Darka
AU  - Žižović, Irena
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3817
AB  - Cellulose acetate films were impregnated with carvacrol using supercritical carbon dioxide. The supercritical impregnation process, conducted in a static regime at pressure of 21 MPa and temperature of 50°C, was optimized by variation in the processing time (30 and 120 min) and decompression rate (from 0.3 MPa/min to 36 MPa/min). Characterization of the obtained cellulose acetate films was performed by Atomic Force Microscopy and Differential Scanning Calorimetry. Effects of glycerol and carvacrol on the properties of the films were discussed. Release kinetics from the cellulose acetate film with 31.4% of carvacrol was investigated in a physiological saline solution. In addition, the Higuchi and Korsmeyer-Peppas release models fitted the carvacrol release curve well. Obtained cellulose acetate films impregnated with carvacrol can be of interest for the application in medicine as wound dressings considering their biocompatibility and biodegradability as well as their potential antimicrobial activity or in the food industry as an active food packaging.
AB  - Filmovi acetata celuloze su impregnirani karvakrolom pomoću natkritičnog ugljenik(IV)-oksida. Proces natkritične impregnacije, izveden u statičkom režimu na pritisku 21 MPa i temperaturi 50°C, je optimizovan varijacijom procesnog vremena (30 i 120 min) i brzine dekompresije (od 0,3 MPa/min do 36 MPa/min). Karakterizacija dobijenih filmova acetata celuloze je izvedena pomoću mikroskopije atomskih sila i diferencijalne skenirajuće kalorimetrije. Efekat glicerola i karvakrola na karakteristike dobijenih filmova je objašnjen. Otpuštanje karvakrola iz filma koji je ima 31,4% primos impregnacije karvakrola je ispitano u fiziološkom rastvoru. Dodatno, Higuči i Korsmejer-Pepas modeli otpuštanja su dobro fitovali krivu otpuštanja karvakrola. Dobijeni filmovi acetata celuloze impregnirani karvakrolom mogu biti značajni za primenu u medicini za previjanje rana uzimajući u obzir njihovu biokompatibilnost i biodegradabilnost kao i potencijalno antimikrobno dejstvo ili u industriji hrane kao aktivno pakovanje.
PB  - Savez inženjera i tehničara Srbije, Beograd
T2  - Tehnika
T1  - Impregnation of cellulose acetate films with carvacrol using supercritical carbon ioxide
T1  - Impregnacija filmova acetata celuloze karvakrolom pomoću natkritičnog ugljenik(IV)-oksida
EP  - 25
IS  - 1
SP  - 19
VL  - 73
DO  - 10.5937/tehnika1801017A
ER  - 

@article{
author = "Adamović, Tijana and Milovanović, Stoja and Marković, Darka and Žižović, Irena",
year = "2018",
abstract = "Cellulose acetate films were impregnated with carvacrol using supercritical carbon dioxide. The supercritical impregnation process, conducted in a static regime at pressure of 21 MPa and temperature of 50°C, was optimized by variation in the processing time (30 and 120 min) and decompression rate (from 0.3 MPa/min to 36 MPa/min). Characterization of the obtained cellulose acetate films was performed by Atomic Force Microscopy and Differential Scanning Calorimetry. Effects of glycerol and carvacrol on the properties of the films were discussed. Release kinetics from the cellulose acetate film with 31.4% of carvacrol was investigated in a physiological saline solution. In addition, the Higuchi and Korsmeyer-Peppas release models fitted the carvacrol release curve well. Obtained cellulose acetate films impregnated with carvacrol can be of interest for the application in medicine as wound dressings considering their biocompatibility and biodegradability as well as their potential antimicrobial activity or in the food industry as an active food packaging., Filmovi acetata celuloze su impregnirani karvakrolom pomoću natkritičnog ugljenik(IV)-oksida. Proces natkritične impregnacije, izveden u statičkom režimu na pritisku 21 MPa i temperaturi 50°C, je optimizovan varijacijom procesnog vremena (30 i 120 min) i brzine dekompresije (od 0,3 MPa/min do 36 MPa/min). Karakterizacija dobijenih filmova acetata celuloze je izvedena pomoću mikroskopije atomskih sila i diferencijalne skenirajuće kalorimetrije. Efekat glicerola i karvakrola na karakteristike dobijenih filmova je objašnjen. Otpuštanje karvakrola iz filma koji je ima 31,4% primos impregnacije karvakrola je ispitano u fiziološkom rastvoru. Dodatno, Higuči i Korsmejer-Pepas modeli otpuštanja su dobro fitovali krivu otpuštanja karvakrola. Dobijeni filmovi acetata celuloze impregnirani karvakrolom mogu biti značajni za primenu u medicini za previjanje rana uzimajući u obzir njihovu biokompatibilnost i biodegradabilnost kao i potencijalno antimikrobno dejstvo ili u industriji hrane kao aktivno pakovanje.",
publisher = "Savez inženjera i tehničara Srbije, Beograd",
journal = "Tehnika",
title = "Impregnation of cellulose acetate films with carvacrol using supercritical carbon ioxide, Impregnacija filmova acetata celuloze karvakrolom pomoću natkritičnog ugljenik(IV)-oksida",
pages = "25-19",
number = "1",
volume = "73",
doi = "10.5937/tehnika1801017A"
}

Adamović, T., Milovanović, S., Marković, D.,& Žižović, I.. (2018). Impregnation of cellulose acetate films with carvacrol using supercritical carbon ioxide. in Tehnika
Savez inženjera i tehničara Srbije, Beograd., 73(1), 19-25.
https://doi.org/10.5937/tehnika1801017A

Adamović T, Milovanović S, Marković D, Žižović I. Impregnation of cellulose acetate films with carvacrol using supercritical carbon ioxide. in Tehnika. 2018;73(1):19-25.
doi:10.5937/tehnika1801017A .

Adamović, Tijana, Milovanović, Stoja, Marković, Darka, Žižović, Irena, "Impregnation of cellulose acetate films with carvacrol using supercritical carbon ioxide" in Tehnika, 73, no. 1 (2018):19-25,
https://doi.org/10.5937/tehnika1801017A . .

TY  - JOUR
AU  - Durdević, Sanja
AU  - Šavikin, Katarina
AU  - Živković, Jelena Č.
AU  - Boehm, Volker
AU  - Stanojković, Tatjana
AU  - Damjanović, Ana
AU  - Petrović, Slobodan
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3961
AB  - Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.
EP  - 232
SP  - 225
VL  - 133
DO  - 10.1016/j.supflu.2017.10.021
ER  - 

@article{
author = "Durdević, Sanja and Šavikin, Katarina and Živković, Jelena Č. and Boehm, Volker and Stanojković, Tatjana and Damjanović, Ana and Petrović, Slobodan",
year = "2018",
abstract = "Pomegranate seeds are an excellent source of compounds with biological activity. In the present study, the pomegranate seed oil (PSO) was tested for antioxidant and cytotoxic potential. PSO was extracted by super-critical fluid extraction with previous treatment of seeds by microwaves and analysed for its fatty acid profile, carotenoid and tocopherol contents. PSO demonstrated to be a good source of polyunsaturated fatty acids (PUFAs) (83.5%), followed by monounsaturated fatty acid (MUFA) and saturated fatty acid (SFA) (9.5% and 7.0%, respectively). Concerning fatty acid profile, punicic (C18:3), oleic (C18:1) and linoleic (C18:2) acids were the predominant ones. Carotenoids and tocopherols were identified and quantified by HPLC. Results showed that the PSO was a good source of gamma-tocopherol as dominant among investigated compounds (128.6 mg/100 g). The extract was screened for its antioxidant activity using alpha-TEAC assay. Cytotoxic effect was determined by MTT test against malignant HeLa, LS174 and A549 cells (IC50 = 49.51-91.54 mu g/mL), and against normal MRC-5 and EA.hy926 cells (IC50  gt  200 mu g/mL). The best effect was exhibited on cervix adenocarcinoma cell line while no cytotoxicity was found for normal cells pointing out PSO safety.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.",
pages = "232-225",
volume = "133",
doi = "10.1016/j.supflu.2017.10.021"
}

Durdević, S., Šavikin, K., Živković, J. Č., Boehm, V., Stanojković, T., Damjanović, A.,& Petrović, S.. (2018). Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 133, 225-232.
https://doi.org/10.1016/j.supflu.2017.10.021

Durdević S, Šavikin K, Živković JČ, Boehm V, Stanojković T, Damjanović A, Petrović S. Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L.. in Journal of Supercritical Fluids. 2018;133:225-232.
doi:10.1016/j.supflu.2017.10.021 .

Durdević, Sanja, Šavikin, Katarina, Živković, Jelena Č., Boehm, Volker, Stanojković, Tatjana, Damjanović, Ana, Petrović, Slobodan, "Antioxidant and cytotoxic activity of fatty oil isolated by supercritical fluid extraction from microwave pretreated seeds of wild growing Punica granatum L." in Journal of Supercritical Fluids, 133 (2018):225-232,
https://doi.org/10.1016/j.supflu.2017.10.021 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Stamenić, Marko
AU  - Radetić, Maja
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Mihaljica, Darko
AU  - Žižović, Irena
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3574
AB  - This study discusses a possibility of cotton fabric impregnation with pyrethrum extract as a tick repellent in supercritical carbon dioxide. A solubility of pyrethrum extract in supercritical carbon dioxide has been determined at 35 and 40 degrees C and pressures ranging from 8 to 20 MPa. The highest solubility of 48.69 kg/m(3) was obtained at temperature of 35 degrees C and pressure of 20 MPa. Density -based equations of Chrastil, Adachi-Lu and del Valle-Aguilera were employed to correlate the experimental data. Based on the solubility data, conditions for the impregnation of cotton fabric were selected. Conditions at 40 degrees C and 8 MPa provided targeted quantities of the impregnated extract of 0.5% and 1% after 1 h and 2 h of the impregnation, respectively. The presence of pyrethrins on the cotton fabric's surface was confirmed by FTIR analysis. Repellent activity of both impregnated cotton fabrics (pyrethrum extract contents of 0.5% and 1%) was proven against ticks.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide
EP  - 72
SP  - 66
VL  - 128
DO  - 10.1016/j.supflu.2017.05.006
ER  - 

@article{
author = "Pajnik, Jelena and Stamenić, Marko and Radetić, Maja and Tomanović, Snežana and Sukara, Ratko and Mihaljica, Darko and Žižović, Irena",
year = "2017",
abstract = "This study discusses a possibility of cotton fabric impregnation with pyrethrum extract as a tick repellent in supercritical carbon dioxide. A solubility of pyrethrum extract in supercritical carbon dioxide has been determined at 35 and 40 degrees C and pressures ranging from 8 to 20 MPa. The highest solubility of 48.69 kg/m(3) was obtained at temperature of 35 degrees C and pressure of 20 MPa. Density -based equations of Chrastil, Adachi-Lu and del Valle-Aguilera were employed to correlate the experimental data. Based on the solubility data, conditions for the impregnation of cotton fabric were selected. Conditions at 40 degrees C and 8 MPa provided targeted quantities of the impregnated extract of 0.5% and 1% after 1 h and 2 h of the impregnation, respectively. The presence of pyrethrins on the cotton fabric's surface was confirmed by FTIR analysis. Repellent activity of both impregnated cotton fabrics (pyrethrum extract contents of 0.5% and 1%) was proven against ticks.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide",
pages = "72-66",
volume = "128",
doi = "10.1016/j.supflu.2017.05.006"
}

Pajnik, J., Stamenić, M., Radetić, M., Tomanović, S., Sukara, R., Mihaljica, D.,& Žižović, I.. (2017). Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 128, 66-72.
https://doi.org/10.1016/j.supflu.2017.05.006

Pajnik J, Stamenić M, Radetić M, Tomanović S, Sukara R, Mihaljica D, Žižović I. Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide. in Journal of Supercritical Fluids. 2017;128:66-72.
doi:10.1016/j.supflu.2017.05.006 .

Pajnik, Jelena, Stamenić, Marko, Radetić, Maja, Tomanović, Snežana, Sukara, Ratko, Mihaljica, Darko, Žižović, Irena, "Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide" in Journal of Supercritical Fluids, 128 (2017):66-72,
https://doi.org/10.1016/j.supflu.2017.05.006 . .

TY  - JOUR
AU  - Milovanović, Stoja
AU  - Adamović, Tijana
AU  - Aksentijević, Ksenija
AU  - Mišić, Dušan
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2017
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3571
AB  - Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.
PB  - Hindawi Ltd, London
T2  - International Journal of Polymer Science
T1  - Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation
VL  - 2017
DO  - 10.1155/2017/8762649
ER  - 

@article{
author = "Milovanović, Stoja and Adamović, Tijana and Aksentijević, Ksenija and Mišić, Dušan and Ivanović, Jasna and Žižović, Irena",
year = "2017",
abstract = "Supercritical CO2 was used as a green solvent and impregnation medium for loading cellulose acetate beads with carvacrol in order to obtain a biomaterial with antibacterial properties. Supercritical solvent impregnation was performed in a high-pressure view cell at temperature of 50 degrees C and pressures of 10, 21, and 30 MPa with the processing time ranging from 2 to 18 h. The rate of impregnation increased with the pressure increase. However, maximum impregnation yield (round 60%) was not affected by the pressure applied. Selected samples of the impregnated cellulose acetate containing 6-60% of carvacrol were proven to have considerable antibacterial effect against Gram-positive and Gram-negative bacterial strains including methicillin-resistant Staphylococcus aureus which causes severe infections in humans and animals. In addition, cellulose acetate beads containing 6.0-33.6% of carvacrol were shown to have a porous structure with submicron pores which is of interest for the controlled delivery applications.",
publisher = "Hindawi Ltd, London",
journal = "International Journal of Polymer Science",
title = "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation",
volume = "2017",
doi = "10.1155/2017/8762649"
}

Milovanović, S., Adamović, T., Aksentijević, K., Mišić, D., Ivanović, J.,& Žižović, I.. (2017). Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science
Hindawi Ltd, London., 2017.
https://doi.org/10.1155/2017/8762649

Milovanović S, Adamović T, Aksentijević K, Mišić D, Ivanović J, Žižović I. Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation. in International Journal of Polymer Science. 2017;2017.
doi:10.1155/2017/8762649 .

Milovanović, Stoja, Adamović, Tijana, Aksentijević, Ksenija, Mišić, Dušan, Ivanović, Jasna, Žižović, Irena, "Cellulose Acetate Based Material with Antibacterial Properties Created by Supercritical Solvent Impregnation" in International Journal of Polymer Science, 2017 (2017),
https://doi.org/10.1155/2017/8762649 . .