Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection

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Study of the effects of soil and irrigation water quality on more efficient agricultural crop production and environment protection (en)
Проучавање утицаја квалитета земљишта и вода за наводњавање на ефикаснију производњу пољопривредних култура и очување животне средине (sr)
Proučavanje uticaja kvaliteta zemljišta i voda za navodnjavanje na efikasniju proizvodnju poljoprivrednih kultura i očuvanje životne sredine (sr_RS)
Authors

Publications

Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production

Buntić, Aneta; Milić, Marija; Stajković-Srbinović, Olivera S.; Rasulić, Nataša V.; Delić, Dušica I.; Mihajlovski, Katarina

(Springer, New York, 2019)

TY  - JOUR
AU  - Buntić, Aneta
AU  - Milić, Marija
AU  - Stajković-Srbinović, Olivera S.
AU  - Rasulić, Nataša V.
AU  - Delić, Dušica I.
AU  - Mihajlovski, Katarina
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4075
AB  - This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.
PB  - Springer, New York
T2  - International Journal of Environmental Science and Technology
T1  - Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production
EP  - 5890
IS  - 10
SP  - 5881
VL  - 16
DO  - 10.1007/s13762-019-02230-9
ER  - 
@article{
author = "Buntić, Aneta and Milić, Marija and Stajković-Srbinović, Olivera S. and Rasulić, Nataša V. and Delić, Dušica I. and Mihajlovski, Katarina",
year = "2019",
abstract = "This study has investigated the valorization of waste tobacco, as lignocellulosic biomass, for cellulase production by rhizobium belonging to genus Sinorhizobium. For the first time, Sinorhizobium meliloti strain 224 was used to produce cellulase (Avicelase and carboxymethyl cellulase) during the submerged and solid-state fermentation using tobacco waste as substrate. The effect of substrate chemical modification on enzymes production has been examined as well. The obtained optimal conditions for the maximum activity of both produced enzymes during submerged fermentation using response surface methodology were: 5 g/L of unmodified waste tobacco concentration, incubation time of 2 days and inoculum concentration of 9%. On the other hand, the use of 1 g of sodium hydroxide modified tobacco for the production of cellulase during solid-state fermentation with 10% inoculum, after 2 days of incubation at 28 degrees C, expressed the maximum Avicelase activity of 1.503 U/g and carboxymethyl cellulase activity of 1.615 U/g. In addition to its basic role in plant root colonization and the provision of nitrogen compounds, strain 224 can also be exploited to produce cellulases by bioconversion of plant waste.",
publisher = "Springer, New York",
journal = "International Journal of Environmental Science and Technology",
title = "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production",
pages = "5890-5881",
number = "10",
volume = "16",
doi = "10.1007/s13762-019-02230-9"
}
Buntić, A., Milić, M., Stajković-Srbinović, O. S., Rasulić, N. V., Delić, D. I.,& Mihajlovski, K.. (2019). Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology
Springer, New York., 16(10), 5881-5890.
https://doi.org/10.1007/s13762-019-02230-9
Buntić A, Milić M, Stajković-Srbinović OS, Rasulić NV, Delić DI, Mihajlovski K. Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production. in International Journal of Environmental Science and Technology. 2019;16(10):5881-5890.
doi:10.1007/s13762-019-02230-9 .
Buntić, Aneta, Milić, Marija, Stajković-Srbinović, Olivera S., Rasulić, Nataša V., Delić, Dušica I., Mihajlovski, Katarina, "Cellulase production by Sinorhizobium meliloti strain 224 using waste tobacco as substrate Utilization of waste tobacco for cellulase production" in International Journal of Environmental Science and Technology, 16, no. 10 (2019):5881-5890,
https://doi.org/10.1007/s13762-019-02230-9 . .
15
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The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment

Buntić, Aneta; Stajković-Srbinović, Olivera S.; Delić, Dušica I.; Dimitrijević-Branković, Suzana; Milić, Marija

(Srpsko hemijsko društvo, Beograd, 2019)

TY  - JOUR
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera S.
AU  - Delić, Dušica I.
AU  - Dimitrijević-Branković, Suzana
AU  - Milić, Marija
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4311
AB  - Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment
EP  - 140
IS  - 2
SP  - 129
VL  - 84
DO  - 10.2298/JSC180802114B
ER  - 
@article{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Delić, Dušica I. and Dimitrijević-Branković, Suzana and Milić, Marija",
year = "2019",
abstract = "Agricultural by-products are generated in large amounts in various industries, creating a serious disposal problem. Valorisation of tobacco waste for the extraction of value-added compounds and the production of enzymes could reduce both the problems of its disposal and the costs of cellulase production. Hitherto, there have been no reported studies concerning the utilization of tobacco residues (after extraction of polyphenols and nicotine, and fermentation pre-treatment by Streptomyces fulvissimus CKS7) for the production of cellulase (CMCase and.vicelase) by Paenibacillus chitinolyticus CKS1. The optimal conditions for the polyphenols and nicotine extraction process were obtained using the response surface methodology: 60 s extraction time in water and 30 mL g(-1) liquid/solid ratio. After the applied bacterial fermentation as a pre-treatment of tobacco residues, using Paenibacillus species, the extraction of polyphenols decreased by up to 10 %, while the extraction of nicotine increased by up to 35 %. Afterward, the maximum of cellulase activities (CMCase of 0.878 U g(-1) and avicelase of 1.417 U g(-1)) were achieved using of the strain CKS1.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment",
pages = "140-129",
number = "2",
volume = "84",
doi = "10.2298/JSC180802114B"
}
Buntić, A., Stajković-Srbinović, O. S., Delić, D. I., Dimitrijević-Branković, S.,& Milić, M.. (2019). The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(2), 129-140.
https://doi.org/10.2298/JSC180802114B
Buntić A, Stajković-Srbinović OS, Delić DI, Dimitrijević-Branković S, Milić M. The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment. in Journal of the Serbian Chemical Society. 2019;84(2):129-140.
doi:10.2298/JSC180802114B .
Buntić, Aneta, Stajković-Srbinović, Olivera S., Delić, Dušica I., Dimitrijević-Branković, Suzana, Milić, Marija, "The production of cellulase from the waste tobacco residues remaining after polyphenols and nicotine extraction and bacterial pre-treatment" in Journal of the Serbian Chemical Society, 84, no. 2 (2019):129-140,
https://doi.org/10.2298/JSC180802114B . .
9
6
6

Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti

Buntić, Aneta; Stajković-Srbinović, Olivera; Milić, Marija; Dubljanin, Tijana; Kuzmanović, Đorđe; Knežević, Magdalena; Delić, Dušica

(The Balkans Scientific Center of the Russian Academy of Natural Sciences, 2019)

TY  - CONF
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera
AU  - Milić, Marija
AU  - Dubljanin, Tijana
AU  - Kuzmanović, Đorđe
AU  - Knežević, Magdalena
AU  - Delić, Dušica
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6415
AB  - Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.
PB  - The Balkans Scientific Center of the Russian Academy of Natural Sciences
C3  - 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection
T1  - Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti
EP  - 163
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6415
ER  - 
@conference{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Dubljanin, Tijana and Kuzmanović, Đorđe and Knežević, Magdalena and Delić, Dušica",
year = "2019",
abstract = "Miscanthus × giganteus, as a lignocellulosic material, consists of about 30%
xylan and represents a good basis for research as a possible raw material in
the production of xylanases. Among the soil bacteria, rhizobium is rarely
investigated as an enzyme producer. For the first time, Sinorhizobium
meliloti strain 207 was used to obtain xylanase during submerged and solid
fermentation using miscanthus biomass as a substrate. During submerged
fermentation (28°C), the maximum xylanase activity was achieved after 48 h
with 10% inoculum and H2SO4 substrate modification. The maximum
xylanase activity of 1.215 U/mL was obtained during solid-state
fermentation (28°C) by using also H2SO4 modified miscanthus biomass
which was moistened with distillate water. The crude enzyme, produced by
strain 207, could be further used in eco-friendly processes of lignocellulose
material bioconversion to useful products.",
publisher = "The Balkans Scientific Center of the Russian Academy of Natural Sciences",
journal = "1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection",
title = "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti",
pages = "163-151",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6415"
}
Buntić, A., Stajković-Srbinović, O., Milić, M., Dubljanin, T., Kuzmanović, Đ., Knežević, M.,& Delić, D.. (2019). Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection
The Balkans Scientific Center of the Russian Academy of Natural Sciences., 151-163.
https://hdl.handle.net/21.15107/rcub_technorep_6415
Buntić A, Stajković-Srbinović O, Milić M, Dubljanin T, Kuzmanović Đ, Knežević M, Delić D. Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti. in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection. 2019;:151-163.
https://hdl.handle.net/21.15107/rcub_technorep_6415 .
Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Dubljanin, Tijana, Kuzmanović, Đorđe, Knežević, Magdalena, Delić, Dušica, "Utilization of miscanthus waste biomass for xylanase production by soil bacterium Sinorhizobium meliloti" in 1st International Symposium: Modern Trends in Agricultural Production and Environmental Protection (2019):151-163,
https://hdl.handle.net/21.15107/rcub_technorep_6415 .

The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination

Buntić, Aneta; Stajković-Srbinović, Olivera S.; Knežević, Magdalena M.; Rasulić, Nataša V.; Kuzmanović, Đorđe Ž.; Dimitrijević-Branković, Suzana; Delić, Dušica I.

(Serbian Soil Science Society, Belgrade, 2019)

TY  - JOUR
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera S.
AU  - Knežević, Magdalena M.
AU  - Rasulić, Nataša V.
AU  - Kuzmanović, Đorđe Ž.
AU  - Dimitrijević-Branković, Suzana
AU  - Delić, Dušica I.
PY  - 2019
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4291
AB  - Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley.
AB  - Zemljišne bakterije iz rizosfere korena biljaka najčešće imaju širok spektar pozitivnog dejstva na biljke I mogu poboljšati njhiov rast. Vrste roda Bacillus jedne su od najistraživanijih bakterija koje mogu doprinositi poboljšanju rasta biljaka. U ovom radu je izolovano sedam Bacillus izolata iz rizosfere lucerke (Medicago sativa). Ispitivana je sposobnost produkcije jedinjenja kao što su vodonik-cijanid, indol-3-sirćetna kiselina I siderofore. Procenjena je I sposobnost indukcije klijavosti na semena pšenice (Triticum aestivum) I ječma (Hordeum jubatum). Izolat BMG2 je proizveo najviše indol-3-sirćetne kiseline (28.89 µg/mL). Dužina korenčića je povećana za 60%, dok je dužina izdanka uvećana 2.23 puta primenom izolata PAZE-6 i BMG1. Pored toga, izolati BMG1, BMG2 i PAZE-6, su poboljšali klijavost obe vrste semena I pokazali sposobnost da proizvedu korisne supstance kao što su sideroforei indol-3sirćetna kiselina. Na dalje bi ovi izolati mogli da se koriste u proizvodnji tečnih aditiva koji bi povećali prinos kultivisanih biljaka, a posebno ječma.
PB  - Serbian Soil Science Society, Belgrade
T2  - Zemljište i biljka
T1  - The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination
T1  - Uticaj bakterijskih izolata iz zemljišta rizosfere na indukciju klijavosti semena pšenice i ječma
EP  - 11
IS  - 2
SP  - 1
VL  - 68
DO  - 10.5937/ZemBilj1902001B
ER  - 
@article{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Knežević, Magdalena M. and Rasulić, Nataša V. and Kuzmanović, Đorđe Ž. and Dimitrijević-Branković, Suzana and Delić, Dušica I.",
year = "2019",
abstract = "Soil microorganisms, which are usually found in plant rhizosphere, have a wide spectrum of beneficial effects on the promotion of plant growth. The most comprehensively studied bacteria with these effects belong to the Bacillus genus. In this study, seven Bacillus isolates from Medicago sativa rhizosphere soils were isolated. Plant growth promoting characteristics of these isolates, such as production of indole-3aceticacid, siderophores and hydrogen cyanide were tested. The induction of wheat (Triticum aestivum) and barley (Hordeum jubatum) seed germination was evaluated in vitro. Isolate BMG2 produced the highest indole-3-aceticacid of 24.89 µg/mL. The length of roots of barley increased up to 60%, while the length of shoots of barley increased 2.23 times afterapplying isolatesPAZE-6 and BMG1, respectively. In addition, isolates BMG1, BMG2 and PAZE-6 improved germination of both types of seeds and showed ability to produce useful substances such as siderophores and indole-3-acetic acid. Further, these isolates could be used in the production of liquid crop additives that can improve the total yield of cultivated plants, especially barley., Zemljišne bakterije iz rizosfere korena biljaka najčešće imaju širok spektar pozitivnog dejstva na biljke I mogu poboljšati njhiov rast. Vrste roda Bacillus jedne su od najistraživanijih bakterija koje mogu doprinositi poboljšanju rasta biljaka. U ovom radu je izolovano sedam Bacillus izolata iz rizosfere lucerke (Medicago sativa). Ispitivana je sposobnost produkcije jedinjenja kao što su vodonik-cijanid, indol-3-sirćetna kiselina I siderofore. Procenjena je I sposobnost indukcije klijavosti na semena pšenice (Triticum aestivum) I ječma (Hordeum jubatum). Izolat BMG2 je proizveo najviše indol-3-sirćetne kiseline (28.89 µg/mL). Dužina korenčića je povećana za 60%, dok je dužina izdanka uvećana 2.23 puta primenom izolata PAZE-6 i BMG1. Pored toga, izolati BMG1, BMG2 i PAZE-6, su poboljšali klijavost obe vrste semena I pokazali sposobnost da proizvedu korisne supstance kao što su sideroforei indol-3sirćetna kiselina. Na dalje bi ovi izolati mogli da se koriste u proizvodnji tečnih aditiva koji bi povećali prinos kultivisanih biljaka, a posebno ječma.",
publisher = "Serbian Soil Science Society, Belgrade",
journal = "Zemljište i biljka",
title = "The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination, Uticaj bakterijskih izolata iz zemljišta rizosfere na indukciju klijavosti semena pšenice i ječma",
pages = "11-1",
number = "2",
volume = "68",
doi = "10.5937/ZemBilj1902001B"
}
Buntić, A., Stajković-Srbinović, O. S., Knežević, M. M., Rasulić, N. V., Kuzmanović, Đ. Ž., Dimitrijević-Branković, S.,& Delić, D. I.. (2019). The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination. in Zemljište i biljka
Serbian Soil Science Society, Belgrade., 68(2), 1-11.
https://doi.org/10.5937/ZemBilj1902001B
Buntić A, Stajković-Srbinović OS, Knežević MM, Rasulić NV, Kuzmanović ĐŽ, Dimitrijević-Branković S, Delić DI. The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination. in Zemljište i biljka. 2019;68(2):1-11.
doi:10.5937/ZemBilj1902001B .
Buntić, Aneta, Stajković-Srbinović, Olivera S., Knežević, Magdalena M., Rasulić, Nataša V., Kuzmanović, Đorđe Ž., Dimitrijević-Branković, Suzana, Delić, Dušica I., "The effect of bacterial isolates from rhizosphere soils on wheat and barley seed germination" in Zemljište i biljka, 68, no. 2 (2019):1-11,
https://doi.org/10.5937/ZemBilj1902001B . .
3

Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates

Buntić, Aneta; Stajković-Srbinović, Olivera; Milić, Marija; Kuzmanović, Đorđe; Rasulić, Nataša; Dimitrijević-Branković, Suzana; Delić, Dušica

(Soil Science Society of Kazakhstan, 2018)

TY  - CONF
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera
AU  - Milić, Marija
AU  - Kuzmanović, Đorđe
AU  - Rasulić, Nataša
AU  - Dimitrijević-Branković, Suzana
AU  - Delić, Dušica
PY  - 2018
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6425
AB  - Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.
PB  - Soil Science Society of Kazakhstan
PB  - Federation of Eurasian Soil Science Societies
C3  - 10th International Soil Science Congress on "Environment and Soil Resources Conservation
T1  - Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates
EP  - 200
SP  - 197
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6425
ER  - 
@conference{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera and Milić, Marija and Kuzmanović, Đorđe and Rasulić, Nataša and Dimitrijević-Branković, Suzana and Delić, Dušica",
year = "2018",
abstract = "Cellulases are industrially important enzymes which can convert the most abundant natural polysaccha-ride - cellulose into glucose, making it as a renewable resource of carbohydrate for the obtaining of bio-based products and bioenergy. The latest trends in microbial cellulase production include the use of various waste products for the microorganism‘s growth, especially of agroindustrial origin, within the solid-state fermentation (SSF), as a promising technology that can potentially reduce the overall costs. Among soil bacteria, rhizobia are rarely investigated as a cellulase producer. The rhizobial soil bacte-rium Sinorhizobium meliloti strain 224 was able to grow and express the metabolic activity on the commercial substrate carboxymethyl cellulose (CMC), but also cellulose based waste materials, such as soybean stems and oat dust. The cellulase produced in a liquid medium with commercial substrates, containing 0.1% (w/v) of CMC in yeast mannitol broth and 10% of the inoculum, after 48 h of incuba-tion, at 28 °C, expressed maximum Avicelase activity of 0.077 U/mL. On the other side, the cellulase produced in a medium with waste substrates, containing 1 g of soybean waste with 10% of the inocu-lum, after 48 h of incubation, at 28 °C, expressed maximum Avicelase activity of 1.295 U/g. The hy-drolysis of Avicel indicate a pre-dominant activity of exoglucanases, those produce the glucose, with traces of other soluble sugars, showing that the crude enzyme, produced on waste material using the soil bacteria S. meliloti 224, could be used in eco-friendly processes of cellulose bioconversion and reduction of biological waste.",
publisher = "Soil Science Society of Kazakhstan, Federation of Eurasian Soil Science Societies",
journal = "10th International Soil Science Congress on "Environment and Soil Resources Conservation",
title = "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates",
pages = "200-197",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6425"
}
Buntić, A., Stajković-Srbinović, O., Milić, M., Kuzmanović, Đ., Rasulić, N., Dimitrijević-Branković, S.,& Delić, D.. (2018). Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation
Soil Science Society of Kazakhstan., 197-200.
https://hdl.handle.net/21.15107/rcub_technorep_6425
Buntić A, Stajković-Srbinović O, Milić M, Kuzmanović Đ, Rasulić N, Dimitrijević-Branković S, Delić D. Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates. in 10th International Soil Science Congress on "Environment and Soil Resources Conservation. 2018;:197-200.
https://hdl.handle.net/21.15107/rcub_technorep_6425 .
Buntić, Aneta, Stajković-Srbinović, Olivera, Milić, Marija, Kuzmanović, Đorđe, Rasulić, Nataša, Dimitrijević-Branković, Suzana, Delić, Dušica, "Sustainable production of cellulase by soil bacterium Sinorhizobium meliloti using commercial and agroindustrial waste substrates" in 10th International Soil Science Congress on "Environment and Soil Resources Conservation (2018):197-200,
https://hdl.handle.net/21.15107/rcub_technorep_6425 .

Influence of spray drying technique on survival of Bradyrhizobium onto sodium alginate based carriers

Buntić, Aneta; Stajković-Srbinović, Olivera S.; Rasulić, Nataša V.; Kuzmanović, Đorđe Ž.; Delić, Dušica I.; Dimitrijević-Branković, Suzana

(Serbian Soil Science Society, Belgrade, 2015)

TY  - JOUR
AU  - Buntić, Aneta
AU  - Stajković-Srbinović, Olivera S.
AU  - Rasulić, Nataša V.
AU  - Kuzmanović, Đorđe Ž.
AU  - Delić, Dušica I.
AU  - Dimitrijević-Branković, Suzana
PY  - 2015
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2946
AB  - Application of microbial inoculants to the soil can improve the nutrient uptake by plants and increase the productivity of the crops. Inoculants should ensure a suitable microenvironment and long survival of bacteria in biological fertilizer and after application to seeds. The immobilization of rhizobia onto a carrier can involve the covering and protecting of the microorganisms. Spray drying is a relatively new immobilization technique for the immobilization of microorganisms onto a carrier and it is rarely used for rhizobia. The survival of bacteria during spray drying immobilization onto alginate based carriers was just 8.1 and 6.8% for Bradyrhizobium spp. strains 542 and 526. The working temperature during drying process was high (outlet 50°C) and it presents more than temperature limit for rhizobia survival. Accordingly, the obtained results were justifiably low in value. The presence of sucrose in liquid inoculant had a positive influence on the survival of rhizobia during immobilization.
PB  - Serbian Soil Science Society, Belgrade
T2  - Zemljište i biljka
T1  - Influence of spray drying technique on survival of Bradyrhizobium onto sodium alginate based carriers
EP  - 16
IS  - 2
SP  - 9
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_technorep_2946
ER  - 
@article{
author = "Buntić, Aneta and Stajković-Srbinović, Olivera S. and Rasulić, Nataša V. and Kuzmanović, Đorđe Ž. and Delić, Dušica I. and Dimitrijević-Branković, Suzana",
year = "2015",
abstract = "Application of microbial inoculants to the soil can improve the nutrient uptake by plants and increase the productivity of the crops. Inoculants should ensure a suitable microenvironment and long survival of bacteria in biological fertilizer and after application to seeds. The immobilization of rhizobia onto a carrier can involve the covering and protecting of the microorganisms. Spray drying is a relatively new immobilization technique for the immobilization of microorganisms onto a carrier and it is rarely used for rhizobia. The survival of bacteria during spray drying immobilization onto alginate based carriers was just 8.1 and 6.8% for Bradyrhizobium spp. strains 542 and 526. The working temperature during drying process was high (outlet 50°C) and it presents more than temperature limit for rhizobia survival. Accordingly, the obtained results were justifiably low in value. The presence of sucrose in liquid inoculant had a positive influence on the survival of rhizobia during immobilization.",
publisher = "Serbian Soil Science Society, Belgrade",
journal = "Zemljište i biljka",
title = "Influence of spray drying technique on survival of Bradyrhizobium onto sodium alginate based carriers",
pages = "16-9",
number = "2",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_technorep_2946"
}
Buntić, A., Stajković-Srbinović, O. S., Rasulić, N. V., Kuzmanović, Đ. Ž., Delić, D. I.,& Dimitrijević-Branković, S.. (2015). Influence of spray drying technique on survival of Bradyrhizobium onto sodium alginate based carriers. in Zemljište i biljka
Serbian Soil Science Society, Belgrade., 64(2), 9-16.
https://hdl.handle.net/21.15107/rcub_technorep_2946
Buntić A, Stajković-Srbinović OS, Rasulić NV, Kuzmanović ĐŽ, Delić DI, Dimitrijević-Branković S. Influence of spray drying technique on survival of Bradyrhizobium onto sodium alginate based carriers. in Zemljište i biljka. 2015;64(2):9-16.
https://hdl.handle.net/21.15107/rcub_technorep_2946 .
Buntić, Aneta, Stajković-Srbinović, Olivera S., Rasulić, Nataša V., Kuzmanović, Đorđe Ž., Delić, Dušica I., Dimitrijević-Branković, Suzana, "Influence of spray drying technique on survival of Bradyrhizobium onto sodium alginate based carriers" in Zemljište i biljka, 64, no. 2 (2015):9-16,
https://hdl.handle.net/21.15107/rcub_technorep_2946 .