TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Tadić, Vanja
AU  - Maksimović, Svetolik
AU  - Filipović, Vladimir
AU  - Dimitrijević-Branković, Suzana
AU  - Miljković, Miona
AU  - Salamon, Ivan
PY  - 2024-02
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7036
AB  - Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.
PB  - Elsevier B.V.
T2  - Sustainable Chemistry and Pharmacy
T1  - Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique
SP  - 101390
VL  - 37
DO  - 10.1016/j.scp.2023.101390
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Tadić, Vanja and Maksimović, Svetolik and Filipović, Vladimir and Dimitrijević-Branković, Suzana and Miljković, Miona and Salamon, Ivan",
year = "2024-02",
abstract = "Within this investigation, the effect of conventional and sophisticated extraction method was evaluated on the chemical composition of black cumin seeds (Nigella sativa L.) essential oils and their bioactive characteristics. Soxhlet extraction at 60 °C by hexane (SEH) and petroleum ether (SEP) was adopted as traditionally used technique, while the supercritical extraction with CO2 (SFE), under the pressure of 10 MPa and temperature of 40 °C, was performed as a modern method, expected to overcome the drawbacks of the conventional solvent extraction. The results showed that SFE enabled up to ten times higher extraction yields, compared to essential oils derived after Soxhlet extraction, however, the bioactive compounds content, such as polyphenols, flavonoids and carotenoids, were extracted in higher quantities in the oil from Soxhlet extraction performance. Thereby, the strongest antioxidant agent was found to be the essential oil obtained from SEP, followed by the oil from SFE and oil obtained from SEH, respectively. The GC-MS analysis revealed that the content of thymoquinone was the highest in the essential oil from SEP (69.58%), and the obtained values are among the highest reported within the available literature data. Also, this oil sample contained about eleven times higher thymoquinone quantities than the essential oil obtained by SFE, which was mostly constituted of a methyl linoleate, a fatty acid methyl ester of linoleic acid. In addition, the essential oil from SEP exerted a value added antimicrobial properties, where it was found to be particularly effective in suppression of the Gram-positive bacteria growth.",
publisher = "Elsevier B.V.",
journal = "Sustainable Chemistry and Pharmacy",
title = "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique",
pages = "101390",
volume = "37",
doi = "10.1016/j.scp.2023.101390"
}

Dimitrijević, S., Milić, M., Tadić, V., Maksimović, S., Filipović, V., Dimitrijević-Branković, S., Miljković, M.,& Salamon, I.. (2024-02). Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy
Elsevier B.V.., 37, 101390.
https://doi.org/10.1016/j.scp.2023.101390

Dimitrijević S, Milić M, Tadić V, Maksimović S, Filipović V, Dimitrijević-Branković S, Miljković M, Salamon I. Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique. in Sustainable Chemistry and Pharmacy. 2024;37:101390.
doi:10.1016/j.scp.2023.101390 .

Dimitrijević, Snežana, Milić, Marija, Tadić, Vanja, Maksimović, Svetolik, Filipović, Vladimir, Dimitrijević-Branković, Suzana, Miljković, Miona, Salamon, Ivan, "Black cumin essential oil as a valuable source of bioactive compounds: Evaluation of the conventional vs. modern extraction technique" in Sustainable Chemistry and Pharmacy, 37 (2024-02):101390,
https://doi.org/10.1016/j.scp.2023.101390 . .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Zille, Andrea
AU  - Ribeiro, Ana Isabel
AU  - Tadić, Vanja
AU  - Ilić-Tomić, Tatjana
AU  - Marković, Darka
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7460
AB  - The syntheses of Ag-based nanoparticles (NPs) with the assistance of plant extracts have been shown to be environmentally benign and cost-effective alternatives to conventional chemical syntheses. This study discusses the application of Paliurus spina-christi, Juglans regia, Humulus lupulus, and Sambucus nigra leaf extracts for in situ synthesis of Ag-based NPs on cotton fabric modified with citric acid. The presence of NPs with an average size ranging from 57 to 99 nm on the fiber surface was confirmed by FESEM. XPS analysis indicated that metallic (Ag0) and/or ionic silver (Ag2O and AgO) appeared on the surface of the modified cotton. The chemical composition, size, shape, and amounts of synthesized NPs were strongly dependent on the applied plant extract. All fabricated nanocomposites exhibited excellent antifungal activity against yeast Candida albicans. Antibacterial activity was significantly stronger against Gram-positive bacteria Staphylococcus aureus than Gram-negative bacteria Escherichia coli. In addition, 99% of silver was retained on the samples after 24 h of contact with physiological saline solution, implying a high stability of nanoparticles. Cytotoxic activity towards HaCaT and MRC5 cells was only observed for the sample synthetized in the presence of H. lupulus extract. Excellent antimicrobial activity and non-cytotoxicity make the developed composites efficient candidates for medicinal applications.
PB  - MDPI
T2  - Molecules
T1  - Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies
IS  - 7
SP  - 1447
VL  - 29
DO  - 10.3390/molecules29071447
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Zille, Andrea and Ribeiro, Ana Isabel and Tadić, Vanja and Ilić-Tomić, Tatjana and Marković, Darka",
year = "2024",
abstract = "The syntheses of Ag-based nanoparticles (NPs) with the assistance of plant extracts have been shown to be environmentally benign and cost-effective alternatives to conventional chemical syntheses. This study discusses the application of Paliurus spina-christi, Juglans regia, Humulus lupulus, and Sambucus nigra leaf extracts for in situ synthesis of Ag-based NPs on cotton fabric modified with citric acid. The presence of NPs with an average size ranging from 57 to 99 nm on the fiber surface was confirmed by FESEM. XPS analysis indicated that metallic (Ag0) and/or ionic silver (Ag2O and AgO) appeared on the surface of the modified cotton. The chemical composition, size, shape, and amounts of synthesized NPs were strongly dependent on the applied plant extract. All fabricated nanocomposites exhibited excellent antifungal activity against yeast Candida albicans. Antibacterial activity was significantly stronger against Gram-positive bacteria Staphylococcus aureus than Gram-negative bacteria Escherichia coli. In addition, 99% of silver was retained on the samples after 24 h of contact with physiological saline solution, implying a high stability of nanoparticles. Cytotoxic activity towards HaCaT and MRC5 cells was only observed for the sample synthetized in the presence of H. lupulus extract. Excellent antimicrobial activity and non-cytotoxicity make the developed composites efficient candidates for medicinal applications.",
publisher = "MDPI",
journal = "Molecules",
title = "Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies",
number = "7",
pages = "1447",
volume = "29",
doi = "10.3390/molecules29071447"
}

Krkobabić, A., Radetić, M., Zille, A., Ribeiro, A. I., Tadić, V., Ilić-Tomić, T.,& Marković, D.. (2024). Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies. in Molecules
MDPI., 29(7), 1447.
https://doi.org/10.3390/molecules29071447

Krkobabić A, Radetić M, Zille A, Ribeiro AI, Tadić V, Ilić-Tomić T, Marković D. Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies. in Molecules. 2024;29(7):1447.
doi:10.3390/molecules29071447 .

Krkobabić, Ana, Radetić, Maja, Zille, Andrea, Ribeiro, Ana Isabel, Tadić, Vanja, Ilić-Tomić, Tatjana, Marković, Darka, "Plant-Assisted Synthesis of Ag-Based Nanoparticles on Cotton: Antimicrobial and Cytotoxicity Studies" in Molecules, 29, no. 7 (2024):1447,
https://doi.org/10.3390/molecules29071447 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gökhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana B.
AU  - Milinčić, Danijel D.
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7457
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
SP  - 518
VL  - 17
DO  - 10.3390/ph17040518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gökhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana B. and Milinčić, Danijel D. and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
pages = "518",
volume = "17",
doi = "10.3390/ph17040518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M. B., Milinčić, D. D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić MB, Milinčić DD, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gökhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana B., Milinčić, Danijel D., Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Buntić, Aneta
AU  - Dimitrijević-Branković, Suzana
AU  - Filipović, Vladimir
AU  - Popović, Vera
AU  - Salamon, Ivan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7344
AB  - Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.
PB  - MDPI
T2  - Sustainability
T1  - Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost
IS  - 4
SP  - 1632
VL  - 16
DO  - 10.3390/su16041632
ER  - 

@article{
author = "Dimitrijević, Snežana and Milić, Marija and Buntić, Aneta and Dimitrijević-Branković, Suzana and Filipović, Vladimir and Popović, Vera and Salamon, Ivan",
year = "2024",
abstract = "Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.",
publisher = "MDPI",
journal = "Sustainability",
title = "Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost",
number = "4",
pages = "1632",
volume = "16",
doi = "10.3390/su16041632"
}

Dimitrijević, S., Milić, M., Buntić, A., Dimitrijević-Branković, S., Filipović, V., Popović, V.,& Salamon, I.. (2024). Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. in Sustainability
MDPI., 16(4), 1632.
https://doi.org/10.3390/su16041632

Dimitrijević S, Milić M, Buntić A, Dimitrijević-Branković S, Filipović V, Popović V, Salamon I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. in Sustainability. 2024;16(4):1632.
doi:10.3390/su16041632 .

Dimitrijević, Snežana, Milić, Marija, Buntić, Aneta, Dimitrijević-Branković, Suzana, Filipović, Vladimir, Popović, Vera, Salamon, Ivan, "Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost" in Sustainability, 16, no. 4 (2024):1632,
https://doi.org/10.3390/su16041632 . .

TY  - DATA
AU  - Dimitrijević, Snežana
AU  - Milić, Marija
AU  - Buntić, Aneta
AU  - Dimitrijević-Branković, Suzana
AU  - Filipović, Vladimir
AU  - Popović, Vera
AU  - Salamon, Ivan
PY  - 2024
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7345
AB  - Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.
PB  - MDPI
T2  - Sustainability
T1  - Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632
IS  - 4
SP  - 1632
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_technorep_7345
ER  - 

@misc{
author = "Dimitrijević, Snežana and Milić, Marija and Buntić, Aneta and Dimitrijević-Branković, Suzana and Filipović, Vladimir and Popović, Vera and Salamon, Ivan",
year = "2024",
abstract = "Composting of mixed medicinal plant waste was performed with the addition of mixture of plant growth promoting bacteria (PGPB), including Streptomyces sp., Paenybacillus sp., Bacillus sp., and Hymenobacter sp., and portions of spent coffee grounds (SCG). To assess the maturity and quality parameters of the compost, chemical and biological parameters (C/N ratio, loss of organic matter, CO2 production, dehydrogenase activity (DHA), and phytotoxicity) were evaluated during the three months of the composting process. The results revealed that the control sample (without the addition of SCG and PGPB) had evidently lower values of DHA during the entire monitoring period in comparison to other samples, indicating lower microbial activity in the compost mixture. Also, according to the released CO2, the composting process was accelerated in all samples where the SCG and PGPB were added, enabling a reduction in time needed for mixed plant waste to decompose. The germination index (GI) of the tested seeds indicated that the produced compost was acceptable and safe, with regard to all of the evaluated samples. However, at the end of the process the samples contained SCG and PGPB, which caused an increase of more than 50% of the GI in comparison to the control sample, expressing a high phyto-stimulant effect and improving the biofertilization impact.",
publisher = "MDPI",
journal = "Sustainability",
title = "Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632",
number = "4",
pages = "1632",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_technorep_7345"
}

Dimitrijević, S., Milić, M., Buntić, A., Dimitrijević-Branković, S., Filipović, V., Popović, V.,& Salamon, I.. (2024). Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632. in Sustainability
MDPI., 16(4), 1632.
https://hdl.handle.net/21.15107/rcub_technorep_7345

Dimitrijević S, Milić M, Buntić A, Dimitrijević-Branković S, Filipović V, Popović V, Salamon I. Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632. in Sustainability. 2024;16(4):1632.
https://hdl.handle.net/21.15107/rcub_technorep_7345 .

Dimitrijević, Snežana, Milić, Marija, Buntić, Aneta, Dimitrijević-Branković, Suzana, Filipović, Vladimir, Popović, Vera, Salamon, Ivan, "Supplementary material for the article: Dimitrijević, S.; Milić, M.; Buntić, A.; Dimitrijević-Branković, S.; Filipović, V.; Popović, V. ; Salamon, I. Spent Coffee Grounds, Plant Growth Promoting Bacteria, and Medicinal Plant Waste: The Biofertilizing Effect of High-Value Compost. Sustainability 2024, 16(4), 1632. https://doi.org/10.3390/su16041632" in Sustainability, 16, no. 4 (2024):1632,
https://hdl.handle.net/21.15107/rcub_technorep_7345 .

TY  - JOUR
AU  - Rajab Elferjane, Muna
AU  - Jovanović, Aleksandra A.
AU  - Milutinović, Violeta
AU  - Čutović, Natalija
AU  - Jovanović Krivokuća, Milica
AU  - Marinković, Aleksandar
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6591
AB  - In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions—HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis. The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin.
PB  - MDPI
T2  - Plants
T1  - From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities
IS  - 14
SP  - 2744
VL  - 12
DO  - 10.3390/plants12142744
ER  - 

@article{
author = "Rajab Elferjane, Muna and Jovanović, Aleksandra A. and Milutinović, Violeta and Čutović, Natalija and Jovanović Krivokuća, Milica and Marinković, Aleksandar",
year = "2023",
abstract = "In the study, the optimization of the extraction from Aloe vera leaf waste was performed via varying solid-to-solvent ratio, solvent type, extraction time, and technique (maceration, heat-, ultrasound-, and microwave-assisted extractions—HAE, UAE, and MAE, respectively). The optimal extraction conditions for achieving the highest polyphenol content are a 1:30 ratio, 70% ethanol, and 30 min of HAE. Total flavonoid and protein contents were significantly higher in the extract from MAE, while total condensed tannin content was the highest in HAE. LC-MS analysis quantified 13 anthraquinone and chromone compounds. The variations in the FT-IR spectra of the extracts obtained by different extraction procedures are minor. The influence of extraction conditions on the antioxidant ability of the extracts depended on applied antioxidant assays. The extracts possessed medium inhibition properties against Staphylococcus aureus and weak inhibitory activity against Enterococcus feacalis. The extracts had stimulative effect on HaCaT cell viability. Regarding the extraction yield, there was a significant difference between the used extraction techniques (MAE > HAE > maceration and UAE). The presented study is an initial step in the production of polyphenol-rich extracts from A. vera leaf waste aimed to be used for the potential preparation of pharmaceutical and cosmetic formulations for the skin.",
publisher = "MDPI",
journal = "Plants",
title = "From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities",
number = "14",
pages = "2744",
volume = "12",
doi = "10.3390/plants12142744"
}

Rajab Elferjane, M., Jovanović, A. A., Milutinović, V., Čutović, N., Jovanović Krivokuća, M.,& Marinković, A.. (2023). From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities. in Plants
MDPI., 12(14), 2744.
https://doi.org/10.3390/plants12142744

Rajab Elferjane M, Jovanović AA, Milutinović V, Čutović N, Jovanović Krivokuća M, Marinković A. From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities. in Plants. 2023;12(14):2744.
doi:10.3390/plants12142744 .

Rajab Elferjane, Muna, Jovanović, Aleksandra A., Milutinović, Violeta, Čutović, Natalija, Jovanović Krivokuća, Milica, Marinković, Aleksandar, "From Aloe vera Leaf Waste to the Extracts with Biological Potential: Optimization of the Extractions, Physicochemical Characterization, and Biological Activities" in Plants, 12, no. 14 (2023):2744,
https://doi.org/10.3390/plants12142744 . .

TY  - JOUR
AU  - Čutović, Natalija
AU  - Batinić, Petar
AU  - Marković, Tatjana
AU  - Radanović, Dragoja
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Jovanović, Aleksandra A.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7041
AB  - The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were ~7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g:50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g:10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g:40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g: 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products.
AB  - Prikazana  studija  je  imala  za  cilj  da  optimizuje  ekstrakciju  polifenola  izbiljkeSatureja  montanaL.  sa oglednog  polja  Instituta  za proučavanjelekovitog bilja „Dr Josif Pančić“, Srbija, variranjem najvažnijih parametara  maceracije,  odnosa biljnog materijala i rastvarača,i  ekstrakcionogvremena.  Dobijeni ekstrakti su okarakterisani u pogleduukupnog sadržaja polifenola (engl.total total polyphenol content, TPC), ukupnog sadržaja flavonoida (engl.total  flavonoid  content,TFC),  antioksidativnog  potencijala, prinosa  ekstrakcije,  provodljivosti,  gustine,  površinskog  napona  i  viskoznosti. Vrednostiukupnog sadržaja polifenola(engl.Total  Polyphenol  Content -TPC)iukupnog sadržaja flavonoida (engl.Total Flavonoid  Content -TFC)su  bile  u  opsezima7,2-92,2  mg ekvivalenta  galne  kiseline  (engl.gallic  acid equivalentGAE) /g i 3,7-10,9 mgekvivalenta katehina(engl.catechinequivalentCE)/g, redom. Najveće antioksidativne  aktivnosti dobijenesu  u  ekstraktima  pripremljenim  u  odnosu biljnog  materijala  i rastvarača1g :50cm-3. Najveći prinos ekstrakcije takođe je dobijenu tom ekstraktu (86,0%), dok vreme ekstrakcije nije imalo značajan uticaj. Najveća  provodljivost  izmerena  je  u  ekstraktu  pripremljenom  u odnosu biljnog materijala i rastvarača1g :10cm-3. Najvećagustina i površinski napon su bili u ekstraktu pripremljenom u odnosu 1g :40cm-3(941 kg m-3i 29,0 mNm-1, redom). Najvećaviskoznostje izmerenau  ekstraktu  pripremljenom  u  odnosu  1g :20cm-3(2,89 mPa∙s). Naša studija pokazuje mogućnosti  za proizvodnju  polifenolima  bogatog  ekstraktabiljkeS.  montanakoji se može koristiti u farmaceutskim, prehrambenim ili kozmetičkim proizvodima.
PB  - Savez hemijskih inženjera Srbije
T2  - Hemijska industrija
T1  - Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts
T1  - Optimizacija procesa ekstrakcije iz biljke Satureja montanal.: fizičkohemijska karakterizacija ekstrakata
EP  - 263
IS  - 4
SP  - 251
VL  - 77
DO  - 10.2298/HEMIND221213020C
ER  - 

@article{
author = "Čutović, Natalija and Batinić, Petar and Marković, Tatjana and Radanović, Dragoja and Marinković, Aleksandar and Bugarski, Branko and Jovanović, Aleksandra A.",
year = "2023",
abstract = "The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were ~7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g:50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g:10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g:40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g: 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products., Prikazana  studija  je  imala  za  cilj  da  optimizuje  ekstrakciju  polifenola  izbiljkeSatureja  montanaL.  sa oglednog  polja  Instituta  za proučavanjelekovitog bilja „Dr Josif Pančić“, Srbija, variranjem najvažnijih parametara  maceracije,  odnosa biljnog materijala i rastvarača,i  ekstrakcionogvremena.  Dobijeni ekstrakti su okarakterisani u pogleduukupnog sadržaja polifenola (engl.total total polyphenol content, TPC), ukupnog sadržaja flavonoida (engl.total  flavonoid  content,TFC),  antioksidativnog  potencijala, prinosa  ekstrakcije,  provodljivosti,  gustine,  površinskog  napona  i  viskoznosti. Vrednostiukupnog sadržaja polifenola(engl.Total  Polyphenol  Content -TPC)iukupnog sadržaja flavonoida (engl.Total Flavonoid  Content -TFC)su  bile  u  opsezima7,2-92,2  mg ekvivalenta  galne  kiseline  (engl.gallic  acid equivalentGAE) /g i 3,7-10,9 mgekvivalenta katehina(engl.catechinequivalentCE)/g, redom. Najveće antioksidativne  aktivnosti dobijenesu  u  ekstraktima  pripremljenim  u  odnosu biljnog  materijala  i rastvarača1g :50cm-3. Najveći prinos ekstrakcije takođe je dobijenu tom ekstraktu (86,0%), dok vreme ekstrakcije nije imalo značajan uticaj. Najveća  provodljivost  izmerena  je  u  ekstraktu  pripremljenom  u odnosu biljnog materijala i rastvarača1g :10cm-3. Najvećagustina i površinski napon su bili u ekstraktu pripremljenom u odnosu 1g :40cm-3(941 kg m-3i 29,0 mNm-1, redom). Najvećaviskoznostje izmerenau  ekstraktu  pripremljenom  u  odnosu  1g :20cm-3(2,89 mPa∙s). Naša studija pokazuje mogućnosti  za proizvodnju  polifenolima  bogatog  ekstraktabiljkeS.  montanakoji se može koristiti u farmaceutskim, prehrambenim ili kozmetičkim proizvodima.",
publisher = "Savez hemijskih inženjera Srbije",
journal = "Hemijska industrija",
title = "Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts, Optimizacija procesa ekstrakcije iz biljke Satureja montanal.: fizičkohemijska karakterizacija ekstrakata",
pages = "263-251",
number = "4",
volume = "77",
doi = "10.2298/HEMIND221213020C"
}

Čutović, N., Batinić, P., Marković, T., Radanović, D., Marinković, A., Bugarski, B.,& Jovanović, A. A.. (2023). Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts. in Hemijska industrija
Savez hemijskih inženjera Srbije., 77(4), 251-263.
https://doi.org/10.2298/HEMIND221213020C

Čutović N, Batinić P, Marković T, Radanović D, Marinković A, Bugarski B, Jovanović AA. Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts. in Hemijska industrija. 2023;77(4):251-263.
doi:10.2298/HEMIND221213020C .

Čutović, Natalija, Batinić, Petar, Marković, Tatjana, Radanović, Dragoja, Marinković, Aleksandar, Bugarski, Branko, Jovanović, Aleksandra A., "Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts" in Hemijska industrija, 77, no. 4 (2023):251-263,
https://doi.org/10.2298/HEMIND221213020C . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Volić, Mina
AU  - Pećinar, Ilinka
AU  - Živković, Jelena
AU  - Šavikin, Katarina P.
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6629
AB  - In the present study, rosehip (Rosa canina L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was −22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome–extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Plants
T1  - Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes
IS  - 17
SP  - 3063
VL  - 12
DO  - 10.3390/plants12173063
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Volić, Mina and Pećinar, Ilinka and Živković, Jelena and Šavikin, Katarina P.",
year = "2023",
abstract = "In the present study, rosehip (Rosa canina L.) extract was successfully encapsulated in phospholipid liposomes using a single-step procedure named the proliposome method. Part of the obtained liposomes was subjected to UV irradiation and non-treated (native) and UV-irradiated liposomes were further characterized in terms of encapsulation efficiency, chemical composition (HPLC analysis), antioxidant capacity, particle size, PDI, zeta potential, conductivity, mobility, and antioxidant capacity. Raman spectroscopy as well as DSC analysis were applied to evaluate the influence of UV irradiation on the physicochemical properties of liposomes. The encapsulation efficiency of extract-loaded liposomes was higher than 90%; the average size was 251.5 nm; the zeta potential was −22.4 mV; and the conductivity was found to be 0.007 mS/cm. UV irradiation did not cause a change in the mentioned parameters. In addition, irradiation did not affect the antioxidant potential of the liposome–extract system. Raman spectroscopy indicated that the extract was completely covered by the lipid membrane during liposome entrapment, and the peroxidation process was minimized by the presence of rosehip extract in liposomes. These results may guide the potential application of rosehip extract-loaded liposomes in the food, pharmaceutical, or cosmetic industries, particularly when liposomal sterilization is needed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Plants",
title = "Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes",
number = "17",
pages = "3063",
volume = "12",
doi = "10.3390/plants12173063"
}

Jovanović, A. A., Balanč, B., Volić, M., Pećinar, I., Živković, J.,& Šavikin, K. P.. (2023). Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes. in Plants
Multidisciplinary Digital Publishing Institute (MDPI)., 12(17), 3063.
https://doi.org/10.3390/plants12173063

Jovanović AA, Balanč B, Volić M, Pećinar I, Živković J, Šavikin KP. Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes. in Plants. 2023;12(17):3063.
doi:10.3390/plants12173063 .

Jovanović, Aleksandra A., Balanč, Bojana, Volić, Mina, Pećinar, Ilinka, Živković, Jelena, Šavikin, Katarina P., "Rosehip Extract-Loaded Liposomes for Potential Skin Application: Physicochemical Properties of Non- and UV-Irradiated Liposomes" in Plants, 12, no. 17 (2023):3063,
https://doi.org/10.3390/plants12173063 . .

TY  - JOUR
AU  - Maksimović, Svetolik
AU  - Stanković, Milica
AU  - Roganović, Sonja
AU  - Nešić, Ivana
AU  - Zvezdanović, Jelena
AU  - Tadić, Vanja
AU  - Žižović, Irena
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5230
AB  - Ethnopharmacological relevance: Helichrysum italicum has been widely used in traditional medicine to treat allergies, colds, cough, skin, liver and gallbladder disorders, inflammation, infections, and sleeplessness. Furthermore, it possesses considerable wound healing and skin protective properties, documented by several in vivo studies performed on animals. However, there is a lack of experimental evidence supporting its potential as a topical agent tested by human clinical trials. Aim of the study: The study aimed to investigate the skin protective activity of cotton gauze and polypropylene non-woven fabric, impregnated with H. italicum extract by the integrated supercritical CO2 extraction-supercritical solvent impregnation process. Materials and methods: The integrated process of supercritical CO2 extraction of H. italicum and the impregnation of cotton gauze and polypropylene non-woven fabric was performed under 350 bar and 40 °C with and without the addition of ethanol as a cosolvent. Impregnated textile materials were tested in vivo for their bioactivity on irritated human skin. Randomized in vivo studies performed involved assays of both safety and efficacy of the impregnated textiles. The effects were evaluated using the in vivo non-invasive biophysical measurements of the following skin parameters: electrical capacitance, transepidermal water loss, melanin index, erythema index, and skin pH. Results: Both cotton gauze and polypropylene non-woven fabric were impregnated with H. italicum extracts under supercritical conditions with considerable values of the impregnation yield (1.97%–4.25%). The addition of ethanol as a cosolvent during the process caused significant changes in the incorporated extracts’ impregnation yield and chemical profile. Both impregnated textile materials were safe, evaluated by their testing on the human skin with no cause of any irritation and redness. However, efficacy studies revealed that polypropylene non-woven fabric impregnated with H. italicum extract with ethanol as a cosolvent, possessed significantly greater potential for skin protection than the other investigated samples. Conclusions: The present study demonstrated the feasibility of the combined supercritical extraction and impregnation process in developing materials for topical application based on H. italicum extract. The results of in vivo studies performed on human volunteers confirmed the suitability of H. italicum active components to be a part of human skin protective preparations because of their ability to maintain the skin unimpaired. Traditionally claimed applications as a medicinal plant capable of regenerating skin have been scientifically proven, in addition to employing green technology in obtaining the impregnated materials with a broad spectrum of utilization.
PB  - Elsevier Ireland Ltd
T2  - Journal of Ethnopharmacology
T1  - Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin
SP  - 115779
VL  - 301
DO  - 10.1016/j.jep.2022.115779
ER  - 

@article{
author = "Maksimović, Svetolik and Stanković, Milica and Roganović, Sonja and Nešić, Ivana and Zvezdanović, Jelena and Tadić, Vanja and Žižović, Irena",
year = "2023",
abstract = "Ethnopharmacological relevance: Helichrysum italicum has been widely used in traditional medicine to treat allergies, colds, cough, skin, liver and gallbladder disorders, inflammation, infections, and sleeplessness. Furthermore, it possesses considerable wound healing and skin protective properties, documented by several in vivo studies performed on animals. However, there is a lack of experimental evidence supporting its potential as a topical agent tested by human clinical trials. Aim of the study: The study aimed to investigate the skin protective activity of cotton gauze and polypropylene non-woven fabric, impregnated with H. italicum extract by the integrated supercritical CO2 extraction-supercritical solvent impregnation process. Materials and methods: The integrated process of supercritical CO2 extraction of H. italicum and the impregnation of cotton gauze and polypropylene non-woven fabric was performed under 350 bar and 40 °C with and without the addition of ethanol as a cosolvent. Impregnated textile materials were tested in vivo for their bioactivity on irritated human skin. Randomized in vivo studies performed involved assays of both safety and efficacy of the impregnated textiles. The effects were evaluated using the in vivo non-invasive biophysical measurements of the following skin parameters: electrical capacitance, transepidermal water loss, melanin index, erythema index, and skin pH. Results: Both cotton gauze and polypropylene non-woven fabric were impregnated with H. italicum extracts under supercritical conditions with considerable values of the impregnation yield (1.97%–4.25%). The addition of ethanol as a cosolvent during the process caused significant changes in the incorporated extracts’ impregnation yield and chemical profile. Both impregnated textile materials were safe, evaluated by their testing on the human skin with no cause of any irritation and redness. However, efficacy studies revealed that polypropylene non-woven fabric impregnated with H. italicum extract with ethanol as a cosolvent, possessed significantly greater potential for skin protection than the other investigated samples. Conclusions: The present study demonstrated the feasibility of the combined supercritical extraction and impregnation process in developing materials for topical application based on H. italicum extract. The results of in vivo studies performed on human volunteers confirmed the suitability of H. italicum active components to be a part of human skin protective preparations because of their ability to maintain the skin unimpaired. Traditionally claimed applications as a medicinal plant capable of regenerating skin have been scientifically proven, in addition to employing green technology in obtaining the impregnated materials with a broad spectrum of utilization.",
publisher = "Elsevier Ireland Ltd",
journal = "Journal of Ethnopharmacology",
title = "Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin",
pages = "115779",
volume = "301",
doi = "10.1016/j.jep.2022.115779"
}

Maksimović, S., Stanković, M., Roganović, S., Nešić, I., Zvezdanović, J., Tadić, V.,& Žižović, I.. (2023). Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin. in Journal of Ethnopharmacology
Elsevier Ireland Ltd., 301, 115779.
https://doi.org/10.1016/j.jep.2022.115779

Maksimović S, Stanković M, Roganović S, Nešić I, Zvezdanović J, Tadić V, Žižović I. Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin. in Journal of Ethnopharmacology. 2023;301:115779.
doi:10.1016/j.jep.2022.115779 .

Maksimović, Svetolik, Stanković, Milica, Roganović, Sonja, Nešić, Ivana, Zvezdanović, Jelena, Tadić, Vanja, Žižović, Irena, "Towards a modern approach to traditional use of Helichrysum italicum in dermatological conditions: In vivo testing supercritical extract on artificially irritated skin" in Journal of Ethnopharmacology, 301 (2023):115779,
https://doi.org/10.1016/j.jep.2022.115779 . .

TY  - JOUR
AU  - Vukmirović, Saša
AU  - Ilić, Vladimirka
AU  - Tadić, Vanja
AU  - Čapo, Ivan
AU  - Pavlović, Nebojša
AU  - Tomas, Ana
AU  - Paut Kusturica, Milica
AU  - Tomić, Nataša
AU  - Maksimović, Svetolik
AU  - Stilinović, Nebojša
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6221
AB  - The framework of this study was a comprehensive investigation of Morus nigra L. extracts, with the aim to establish the correlation between chemical composition and antioxidant/hepatoprotective activity of a series of black mulberry extracts obtained from aerial parts of the plant. Black mulberry leaf (MLEE), bark (MBEE), juice (MJ) and fresh fruit (MFEE) extracts were obtained using the conventional Soxhlet extraction, while the supercritical CO2 extraction procedure was employed for preparation of the seed oil (MSO). Analysis of the chemical composition was performed using spectrophotometric, HPLC and GC methods. For the evaluation of antioxidant activity, in vitro FRAP and DPPH assays were applied. In Haan strain NMRI mice with streptozotocin-induced oxidative stress, in vivo antioxidant activity and liver tissue integrity were examined. The content of polyphenolic compounds was the highest in MBEE (68.3 ± 0.7 mgGAE/g) with the most abundant compounds being polyphenolic acids, followed by MLEE (23.4 ± 0.5 mgGAE/g) with the flavonoids isoquercetin and rutin being present in a significant amount. An analysis of MSO revealed a high content of γ-linoleic acid. The highest antioxidant activity in vitro (FRAP and DPPH) was observed for MLEE, MBEE and MSO. Beneficial effects were confirmed in vivo, with lower values of hepatosomatic index, potentiation of the activity of the enzymes superoxide dismutase and catalase, a lower rate of lipid peroxidation and reduced positivity for the P450 enzyme in animals treated with MLEE, MBEE and MSO. Black mulberry leaf and bark extracts as well as seed oil exhibited significant antioxidant activity. Apart from the confirmed biological properties of the fruit and leaf extracts, the observed activities of black mulberry seed oil and bark extract imply its importance as a sustainable source of phytochemicals.
PB  - MDPI
T2  - Antioxidants
T1  - Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.
IS  - 2
SP  - 382
VL  - 12
DO  - 10.3390/antiox12020382
ER  - 

@article{
author = "Vukmirović, Saša and Ilić, Vladimirka and Tadić, Vanja and Čapo, Ivan and Pavlović, Nebojša and Tomas, Ana and Paut Kusturica, Milica and Tomić, Nataša and Maksimović, Svetolik and Stilinović, Nebojša",
year = "2023",
abstract = "The framework of this study was a comprehensive investigation of Morus nigra L. extracts, with the aim to establish the correlation between chemical composition and antioxidant/hepatoprotective activity of a series of black mulberry extracts obtained from aerial parts of the plant. Black mulberry leaf (MLEE), bark (MBEE), juice (MJ) and fresh fruit (MFEE) extracts were obtained using the conventional Soxhlet extraction, while the supercritical CO2 extraction procedure was employed for preparation of the seed oil (MSO). Analysis of the chemical composition was performed using spectrophotometric, HPLC and GC methods. For the evaluation of antioxidant activity, in vitro FRAP and DPPH assays were applied. In Haan strain NMRI mice with streptozotocin-induced oxidative stress, in vivo antioxidant activity and liver tissue integrity were examined. The content of polyphenolic compounds was the highest in MBEE (68.3 ± 0.7 mgGAE/g) with the most abundant compounds being polyphenolic acids, followed by MLEE (23.4 ± 0.5 mgGAE/g) with the flavonoids isoquercetin and rutin being present in a significant amount. An analysis of MSO revealed a high content of γ-linoleic acid. The highest antioxidant activity in vitro (FRAP and DPPH) was observed for MLEE, MBEE and MSO. Beneficial effects were confirmed in vivo, with lower values of hepatosomatic index, potentiation of the activity of the enzymes superoxide dismutase and catalase, a lower rate of lipid peroxidation and reduced positivity for the P450 enzyme in animals treated with MLEE, MBEE and MSO. Black mulberry leaf and bark extracts as well as seed oil exhibited significant antioxidant activity. Apart from the confirmed biological properties of the fruit and leaf extracts, the observed activities of black mulberry seed oil and bark extract imply its importance as a sustainable source of phytochemicals.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.",
number = "2",
pages = "382",
volume = "12",
doi = "10.3390/antiox12020382"
}

Vukmirović, S., Ilić, V., Tadić, V., Čapo, I., Pavlović, N., Tomas, A., Paut Kusturica, M., Tomić, N., Maksimović, S.,& Stilinović, N.. (2023). Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. in Antioxidants
MDPI., 12(2), 382.
https://doi.org/10.3390/antiox12020382

Vukmirović S, Ilić V, Tadić V, Čapo I, Pavlović N, Tomas A, Paut Kusturica M, Tomić N, Maksimović S, Stilinović N. Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. in Antioxidants. 2023;12(2):382.
doi:10.3390/antiox12020382 .

Vukmirović, Saša, Ilić, Vladimirka, Tadić, Vanja, Čapo, Ivan, Pavlović, Nebojša, Tomas, Ana, Paut Kusturica, Milica, Tomić, Nataša, Maksimović, Svetolik, Stilinović, Nebojša, "Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L." in Antioxidants, 12, no. 2 (2023):382,
https://doi.org/10.3390/antiox12020382 . .

TY  - JOUR
AU  - Krkobabić, Ana
AU  - Radetić, Maja
AU  - Tseng, Hsiang-Han
AU  - Nunney, Tim S.
AU  - Tadić, Vanja
AU  - Ilić-Tomić, Tatjana
AU  - Marković, Darka
PY  - 2023
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5286
AB  - The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.
PB  - Elsevier B.V.
T2  - Applied Surface Science
T1  - Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract
SP  - 155612
VL  - 611
DO  - 10.1016/j.apsusc.2022.155612
ER  - 

@article{
author = "Krkobabić, Ana and Radetić, Maja and Tseng, Hsiang-Han and Nunney, Tim S. and Tadić, Vanja and Ilić-Tomić, Tatjana and Marković, Darka",
year = "2023",
abstract = "The demand for medical textiles in various forms with strong antimicrobial activity drastically increased during the COVID19 pandemic. In an attempt to tackle this issue and to develop antimicrobial textiles in more environmentally benign manner, a viscose fabric after coating with biopolymer chitosan has been impregnated with Ag- and Cu-based nanoparticles. Chitosan was applied in the presence and absence of cross-linker 1,2,3,4-butanetetracarboxylic acid (BTCA). In situ green synthesis of nanoparticles was performed using a Punica granatum (pomegranate) peel extract as a reducing and stabilizing agent. Formation of nanoparticles on the fiber surface was confirmed by FESEM. Elemental analysis by XPS showed the synthesized nanoparticles exist as AgCl and a mixture of Cu/CuO/Cu2O in the modified samples. Moreover, these nanoparticles appeared to be present not only on the sample surface but also buried within the fibers, as indicated by XPS mapping and depth profiling measurements. All impregnated fabrics exhibited excellent antifungal activity providing the maximum reduction of yeast Candida albicans colonies. Antibacterial activity was stronger against Gram-negative bacteria Escherichia coli than Gram-positive bacteria Staphylococcus aureus, and it was highly influenced by metal content. The fabrics impregnated with AgCl nanoparticles showed lower cytotoxicity towards human keratinocyte cells.",
publisher = "Elsevier B.V.",
journal = "Applied Surface Science",
title = "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract",
pages = "155612",
volume = "611",
doi = "10.1016/j.apsusc.2022.155612"
}

Krkobabić, A., Radetić, M., Tseng, H., Nunney, T. S., Tadić, V., Ilić-Tomić, T.,& Marković, D.. (2023). Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science
Elsevier B.V.., 611, 155612.
https://doi.org/10.1016/j.apsusc.2022.155612

Krkobabić A, Radetić M, Tseng H, Nunney TS, Tadić V, Ilić-Tomić T, Marković D. Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract. in Applied Surface Science. 2023;611:155612.
doi:10.1016/j.apsusc.2022.155612 .

Krkobabić, Ana, Radetić, Maja, Tseng, Hsiang-Han, Nunney, Tim S., Tadić, Vanja, Ilić-Tomić, Tatjana, Marković, Darka, "Green in situ synthesis of Ag- and Cu-based nanoparticles on viscose fabric using a Punica granatum peel extract" in Applied Surface Science, 611 (2023):155612,
https://doi.org/10.1016/j.apsusc.2022.155612 . .

TY  - JOUR
AU  - Dimitrijević, Snežana
AU  - Filipović, Vladimir
AU  - Milić, Marija
AU  - Dimitrijević-Branković, Suzana
AU  - Ugrenović, Vladan
AU  - Popović, Vera
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6412
AB  - U cilju proučavanja uticaja bakterija koje stimulišu rast biljaka, a koje pripadaju rodu Strep-
tomices sp , Paenibacillus sp i Hymenibacter sp , na prinos i kvalitet semena uljanog lana i crnog
kima, kao i na morfološke karakteristike biljaka, sprovedeni su dvogodišnji terenski eksperimen-
ti Tretman zemljišta mešanom kulturom bakterija različitih koncentracija je primenjen tokom
setve biljaka Pre početka setve ispitan je uticaj pojedinačnih sojeva na indeks klijavosti seme-
na uljanog lana i crnog kima Na osnovu rezultata prikazanih u radu, može se uočiti da sojevi,
CKS1 – Paenybacillus chitinolyticus, CKS3 – Hymenobacter sp , CKS7 – Streptomyces fulvissimus,
u svim ispitivanim koncentracijama, daju vrednosti indeksa klijavosti GI preko 100% kod uljanog
lana, dok je kod crnog kima takav efekat pokazao samo soj CKS7 Pozitivno dejstvo bakterijskog
preparata kod obe ispitane biljne vrste zabeleženo je merenjem prinosa semena po hektaru tre-
tiranom manjom koncentracijom bakterijskog preparata, u odnosu na odgovarajuće netretirane
uzorke (kontrolu) Pozitivna razlika zabeležena je merenjem broja čaura u uzorcima tretiranog
uljanog lana i crnog kima u odnosu na netretirani, kao i mase korena obe tretirane biljne vrste
Takođe, najveću ukupnu klijavost u odnosu na kontrolu je ostvarila varijanta uljanog lana L2 u
tretmanu manjom koncentracijom bakterija od 98,3±1,4%.
PB  - Serbian Association of Plant Breedings and Seed Producers, Belgrade
T2  - Selekcija i semenarstvo
T1  - Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima
EP  - 38
IS  - 2
SP  - 30
VL  - 28
DO  - 10.5937/SelSem2202030D
ER  - 

@article{
author = "Dimitrijević, Snežana and Filipović, Vladimir and Milić, Marija and Dimitrijević-Branković, Suzana and Ugrenović, Vladan and Popović, Vera",
year = "2022",
abstract = "U cilju proučavanja uticaja bakterija koje stimulišu rast biljaka, a koje pripadaju rodu Strep-
tomices sp , Paenibacillus sp i Hymenibacter sp , na prinos i kvalitet semena uljanog lana i crnog
kima, kao i na morfološke karakteristike biljaka, sprovedeni su dvogodišnji terenski eksperimen-
ti Tretman zemljišta mešanom kulturom bakterija različitih koncentracija je primenjen tokom
setve biljaka Pre početka setve ispitan je uticaj pojedinačnih sojeva na indeks klijavosti seme-
na uljanog lana i crnog kima Na osnovu rezultata prikazanih u radu, može se uočiti da sojevi,
CKS1 – Paenybacillus chitinolyticus, CKS3 – Hymenobacter sp , CKS7 – Streptomyces fulvissimus,
u svim ispitivanim koncentracijama, daju vrednosti indeksa klijavosti GI preko 100% kod uljanog
lana, dok je kod crnog kima takav efekat pokazao samo soj CKS7 Pozitivno dejstvo bakterijskog
preparata kod obe ispitane biljne vrste zabeleženo je merenjem prinosa semena po hektaru tre-
tiranom manjom koncentracijom bakterijskog preparata, u odnosu na odgovarajuće netretirane
uzorke (kontrolu) Pozitivna razlika zabeležena je merenjem broja čaura u uzorcima tretiranog
uljanog lana i crnog kima u odnosu na netretirani, kao i mase korena obe tretirane biljne vrste
Takođe, najveću ukupnu klijavost u odnosu na kontrolu je ostvarila varijanta uljanog lana L2 u
tretmanu manjom koncentracijom bakterija od 98,3±1,4%.",
publisher = "Serbian Association of Plant Breedings and Seed Producers, Belgrade",
journal = "Selekcija i semenarstvo",
title = "Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima",
pages = "38-30",
number = "2",
volume = "28",
doi = "10.5937/SelSem2202030D"
}

Dimitrijević, S., Filipović, V., Milić, M., Dimitrijević-Branković, S., Ugrenović, V.,& Popović, V.. (2022). Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima. in Selekcija i semenarstvo
Serbian Association of Plant Breedings and Seed Producers, Belgrade., 28(2), 30-38.
https://doi.org/10.5937/SelSem2202030D

Dimitrijević S, Filipović V, Milić M, Dimitrijević-Branković S, Ugrenović V, Popović V. Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima. in Selekcija i semenarstvo. 2022;28(2):30-38.
doi:10.5937/SelSem2202030D .

Dimitrijević, Snežana, Filipović, Vladimir, Milić, Marija, Dimitrijević-Branković, Suzana, Ugrenović, Vladan, Popović, Vera, "Ispitivanje fitostimulatornog delovanja bakterijskih sojeva tokom gajenja uljanog lana i crnog kima" in Selekcija i semenarstvo, 28, no. 2 (2022):30-38,
https://doi.org/10.5937/SelSem2202030D . .

Filipović, V., Ugrenović, V., Maksimović, Z., Dimitrijević, S., Milić, M., Popović, V.,& Terzić, D.. (2022). Vegetativno razmnožavanje panonskog timijana (Thymus pannonicus All.) uz primenu fitohormona. .
https://hdl.handle.net/21.15107/rcub_technorep_6454

Filipović V, Ugrenović V, Maksimović Z, Dimitrijević S, Milić M, Popović V, Terzić D. Vegetativno razmnožavanje panonskog timijana (Thymus pannonicus All.) uz primenu fitohormona. 2022;.
https://hdl.handle.net/21.15107/rcub_technorep_6454 .

Filipović, Vladimir, Ugrenović, Vladan, Maksimović, Zoran, Dimitrijević, Snežana, Milić, Marija, Popović, Vera, Terzić, Dragan, "Vegetativno razmnožavanje panonskog timijana (Thymus pannonicus All.) uz primenu fitohormona" (2022),
https://hdl.handle.net/21.15107/rcub_technorep_6454 .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Vajić, Una-Jovana V.
AU  - Mijin, Dušan Ž.
AU  - Zdunić, Gordana M.
AU  - Šavikin, Katarina P.
AU  - Branković, Suzana
AU  - Kitić, Dušanka
AU  - Bugarski, Branko M.
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5194
AB  - In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products
PB  - Elsevier GmbH
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract
SP  - 100417
VL  - 31
DO  - 10.1016/j.jarmap.2022.100417
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Vajić, Una-Jovana V. and Mijin, Dušan Ž. and Zdunić, Gordana M. and Šavikin, Katarina P. and Branković, Suzana and Kitić, Dušanka and Bugarski, Branko M.",
year = "2022",
abstract = "In the present study, polyphenol extraction from Thymus serpyllum by-product (herbal dust) in microwave reactor was optimized through varying extraction time, ethanol concentration and solid-to-solvent ratiovia determination of total polyphenol and flavonoid contents, and antioxidant activity, using response surface methodology and a central composite design. Total polyphenol and flavonoids contents, as well as antioxidant activity of the extracts were statistically significantly affected by ethanol concentration and solid-to-solvent ratio. However, extraction time had statistically significant influence only on polyphenol yield. The extraction conditions that maximized polyphenol recovery were 48% ethanol and 0.0402 g/mL solid-to-solvent ratio during 86 s of microwave-assisted extraction, and under these conditions total polyphenol content was estimated to be 57.2 mg of gallic acid equivalents (GAE)/L, while measured value was 58.1 ± 3.2 mg GAE/L. Polyphenol content (HPLC analysis), antioxidant, antimicrobial and antispasmodic potential of the extract prepared under these optimal extraction conditions were evaluated. Rosmarinic acids, luteolin 7-O-glucuronide, caffeic acid, apigenin glucuronide, 6-hydroxyluteolin 7-O-glucoside, 6,8-di-C-glucosylapigenin, and traces of chlorogenic acid were quantified in the extract (descending order of content). The selected extract inhibited 95% ± 0.9 of lipid peroxidation in β-Carotene and linoleic acid emulsion. It was more effective against tested Gram-positive bacteria than against Gram-negative and showed dose-dependent antispasmodic activity on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions. Microwave-assisted extraction could be selected as a successful technique for polyphenol extraction from wild thyme by-product. Biological activities shown in the study open the possibility of potential application of the extract in pharmaceutical, food, functional food and cosmetic products",
publisher = "Elsevier GmbH",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract",
pages = "100417",
volume = "31",
doi = "10.1016/j.jarmap.2022.100417"
}

Jovanović, A. A., Vajić, U. V., Mijin, D. Ž., Zdunić, G. M., Šavikin, K. P., Branković, S., Kitić, D.,& Bugarski, B. M.. (2022). Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants
Elsevier GmbH., 31, 100417.
https://doi.org/10.1016/j.jarmap.2022.100417

Jovanović AA, Vajić UV, Mijin DŽ, Zdunić GM, Šavikin KP, Branković S, Kitić D, Bugarski BM. Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract. in Journal of Applied Research on Medicinal and Aromatic Plants. 2022;31:100417.
doi:10.1016/j.jarmap.2022.100417 .

Jovanović, Aleksandra A., Vajić, Una-Jovana V., Mijin, Dušan Ž., Zdunić, Gordana M., Šavikin, Katarina P., Branković, Suzana, Kitić, Dušanka, Bugarski, Branko M., "Polyphenol extraction in microwave reactor using by-product of Thymus serpyllum L. and biological potential of the extract" in Journal of Applied Research on Medicinal and Aromatic Plants, 31 (2022):100417,
https://doi.org/10.1016/j.jarmap.2022.100417 . .

TY  - JOUR
AU  - Vajić, Una-Jovana
AU  - Živković, Jelena
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5561
AB  - The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
EP  - 128
IS  - 1
SP  - 119
VL  - 41
DO  - 10.20450/mjcce.2022.2238
ER  - 

@article{
author = "Vajić, Una-Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "The aim of this study was to optimize the parameters for the extraction of total flavonoids from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless of extraction time, aqueous methanolic extracts had higher total flavonoid content than did aqueous ethanolic extracts. So, full factorial design and response surface methodology (RSM) were em-ployed to estimate the effects of methanol content (50, 75 and 100 %) and extraction time (30, 60 and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol content in the solvent influenced significantly total flavonoid content and FRAP (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69 % aqueous methanol and 67 min, and 65 % aqueous methanol and 83 min for maximal FRAP antioxidant capacity. DPPH (2,2-diphenyl-1-picrylhydrazyl) antioxidant capacity was not significantly affected by extraction time or methanol percentage in the solvent.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology",
pages = "128-119",
number = "1",
volume = "41",
doi = "10.20450/mjcce.2022.2238"
}

Vajić, U., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 41(1), 119-128.
https://doi.org/10.20450/mjcce.2022.2238

Vajić U, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128.
doi:10.20450/mjcce.2022.2238 .

Vajić, Una-Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128,
https://doi.org/10.20450/mjcce.2022.2238 . .

TY  - CONF
AU  - Filipović, Vladimir
AU  - Ugrenović, Vladan
AU  - Maksimović, Zoran
AU  - Dimitrijević, Snežana
AU  - Popović, Vera
AU  - Mihajlovski, Katarina
AU  - Milić, Marija
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6424
AB  - This study has explored the effect of three phytohormones application, based on α-naphthyl acetic acid, named INCIT 1, INCIT 8 and INCIT K, on the root system growing potential and rooting stimulation of Thymus pannonicus All. cuttings. This plant is highly reputable not only in a folk medicine, but also recognized in the business sector, due to a multiple uses and beneficial effects on human health.
The research was located in Veliko Središte, Vršac (Serbia) and conducted in May, 2021. The cuttings were dipped in a powdered formulation of phytohormones and placed in plastic containers pre-filled with commercial substrate. The phytohormone-free variant was taken as a control. After 2 months, the cuttings were removed and the morphological and biological properties (polyphenols content and antioxidant activity by DPPH radical and FRAP method) were measured.
Based on the morphological results, all of treated samples showed satisfactory behaviour, whereby the most suitable phytohormone to stimulate the growth of cuttings was INCIT 8, in which the average weight of the aboveground vegetative part of the plant and root mass were higher for more than 60% in relation to the control; the number of roots were about 25% higher, whereby the mass of the whole plant was almost 3 times increased. Considering the biological activities in a 5% aqueous infusions of the samples, it was found that the polyphenols content and the antioxidant activity were lowered in treated samples, with relation to the control, excluding the sample INCIT 8 where the FRAP value was slightly increased, while the inhibition of DPPH radicals was higher by around 15%.
The applied phytohormone treatment may ensure economically viable yields of standard or even improved plant quality. In the following period, it is expected that all of treated adult plants will have a higher content of biologically active substances.
PB  - University of Belgrade
C3  - UNIFood Conference
T1  - The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6424
ER  - 

@conference{
author = "Filipović, Vladimir and Ugrenović, Vladan and Maksimović, Zoran and Dimitrijević, Snežana and Popović, Vera and Mihajlovski, Katarina and Milić, Marija",
year = "2021",
abstract = "This study has explored the effect of three phytohormones application, based on α-naphthyl acetic acid, named INCIT 1, INCIT 8 and INCIT K, on the root system growing potential and rooting stimulation of Thymus pannonicus All. cuttings. This plant is highly reputable not only in a folk medicine, but also recognized in the business sector, due to a multiple uses and beneficial effects on human health.
The research was located in Veliko Središte, Vršac (Serbia) and conducted in May, 2021. The cuttings were dipped in a powdered formulation of phytohormones and placed in plastic containers pre-filled with commercial substrate. The phytohormone-free variant was taken as a control. After 2 months, the cuttings were removed and the morphological and biological properties (polyphenols content and antioxidant activity by DPPH radical and FRAP method) were measured.
Based on the morphological results, all of treated samples showed satisfactory behaviour, whereby the most suitable phytohormone to stimulate the growth of cuttings was INCIT 8, in which the average weight of the aboveground vegetative part of the plant and root mass were higher for more than 60% in relation to the control; the number of roots were about 25% higher, whereby the mass of the whole plant was almost 3 times increased. Considering the biological activities in a 5% aqueous infusions of the samples, it was found that the polyphenols content and the antioxidant activity were lowered in treated samples, with relation to the control, excluding the sample INCIT 8 where the FRAP value was slightly increased, while the inhibition of DPPH radicals was higher by around 15%.
The applied phytohormone treatment may ensure economically viable yields of standard or even improved plant quality. In the following period, it is expected that all of treated adult plants will have a higher content of biologically active substances.",
publisher = "University of Belgrade",
journal = "UNIFood Conference",
title = "The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all",
pages = "151",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6424"
}

Filipović, V., Ugrenović, V., Maksimović, Z., Dimitrijević, S., Popović, V., Mihajlovski, K.,& Milić, M.. (2021). The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all. in UNIFood Conference
University of Belgrade., 151.
https://hdl.handle.net/21.15107/rcub_technorep_6424

Filipović V, Ugrenović V, Maksimović Z, Dimitrijević S, Popović V, Mihajlovski K, Milić M. The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all. in UNIFood Conference. 2021;:151.
https://hdl.handle.net/21.15107/rcub_technorep_6424 .

Filipović, Vladimir, Ugrenović, Vladan, Maksimović, Zoran, Dimitrijević, Snežana, Popović, Vera, Mihajlovski, Katarina, Milić, Marija, "The effect of phytohormones application on morphological and biological properties of Thymus pannonicus all" in UNIFood Conference (2021):151,
https://hdl.handle.net/21.15107/rcub_technorep_6424 .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Đorđević, Verica
AU  - Petrović, Predrag
AU  - Pljevljakusic, Dejan S.
AU  - Zdunic, Gordana M.
AU  - Savikin, Katarina P.
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4794
AB  - The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.
T2  - Journal of Applied Research on Medicinal and Aromatic Plants
T1  - The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme
VL  - 25
DO  - 10.1016/j.jarmap.2021.100328
ER  - 

@article{
author = "Jovanović, Aleksandra and Đorđević, Verica and Petrović, Predrag and Pljevljakusic, Dejan S. and Zdunic, Gordana M. and Savikin, Katarina P. and Bugarski, Branko",
year = "2021",
abstract = "The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 +/- 0.1 mg gallic acid equivalents (GAE)/L and 16.8 +/- 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucumnide and salvianolic acid I (185.2 +/- 7.3, 141.6 +/- 3.4 and 76.4 +/- 2.1 mu g/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.",
journal = "Journal of Applied Research on Medicinal and Aromatic Plants",
title = "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme",
volume = "25",
doi = "10.1016/j.jarmap.2021.100328"
}

Jovanović, A., Đorđević, V., Petrović, P., Pljevljakusic, D. S., Zdunic, G. M., Savikin, K. P.,& Bugarski, B.. (2021). The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants, 25.
https://doi.org/10.1016/j.jarmap.2021.100328

Jovanović A, Đorđević V, Petrović P, Pljevljakusic DS, Zdunic GM, Savikin KP, Bugarski B. The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme. in Journal of Applied Research on Medicinal and Aromatic Plants. 2021;25.
doi:10.1016/j.jarmap.2021.100328 .

Jovanović, Aleksandra, Đorđević, Verica, Petrović, Predrag, Pljevljakusic, Dejan S., Zdunic, Gordana M., Savikin, Katarina P., Bugarski, Branko, "The influence of different extraction conditions on polyphenol content, antioxidant and antimicrobial activities of wild thyme" in Journal of Applied Research on Medicinal and Aromatic Plants, 25 (2021),
https://doi.org/10.1016/j.jarmap.2021.100328 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Zdunic, Gordana M.
AU  - Savikin, Katarina P.
AU  - Kitic, Dusanka
AU  - Đorđević, Verica
AU  - Bugarski, Branko
AU  - Brankovic, Suzana
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4798
AB  - In the presented study, polyphenol profile, spasmolytic, antimicrobial and antioxidant properties of lyophilized Thymus serpyllum extracts were examined. The main phenolic acid in the extracts was rosmarinic acid, followed by salvianolic acid I, salvianolic acid K isomer and caffeic acid, whereas chlorogenic acid was detected only in traces; the dominant flavonoids were luteolin 7-O-glucuronide and 6-hydroxyluteolin 7-O-glucoside, while the contents of apigenin glucuronide and 6,8-di-C-glucosylapigenin were significantly lower. All tested extracts have shown spasmolytic effect in a dose-dependent manner on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions; lyophilized extract obtained in maceration has shown significantly better spasmolytic activity. The mentioned extract has shown dosedependent spasmolytic effect in the model of contractions induced by barium chloride and calcium chloride, as well. Furthermore, lyophilized extracts were effective against Gram-positive bacteria and showed only moderate antifungal activity. Ultrasound-assisted extraction provided extracts with better ability of neutralization DPPH radicals, whereas maceration and ultrasound-assisted extraction approved to be better regarding beta-carotene bleaching assay. Due to spasmolytic, antimicrobial and antioxidant properties shown in the present study, polyphenols-rich T. serpyllum extract may be used as adjuvant in therapy of gastrointestinal disorders and infections caused by some of the susceptible pathogens.
T2  - South African Journal of Botany
T1  - Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties
EP  - 283
SP  - 274
VL  - 142
DO  - 10.1016/j.sajb.2021.06.028
ER  - 

@article{
author = "Jovanović, Aleksandra and Petrović, Predrag and Zdunic, Gordana M. and Savikin, Katarina P. and Kitic, Dusanka and Đorđević, Verica and Bugarski, Branko and Brankovic, Suzana",
year = "2021",
abstract = "In the presented study, polyphenol profile, spasmolytic, antimicrobial and antioxidant properties of lyophilized Thymus serpyllum extracts were examined. The main phenolic acid in the extracts was rosmarinic acid, followed by salvianolic acid I, salvianolic acid K isomer and caffeic acid, whereas chlorogenic acid was detected only in traces; the dominant flavonoids were luteolin 7-O-glucuronide and 6-hydroxyluteolin 7-O-glucoside, while the contents of apigenin glucuronide and 6,8-di-C-glucosylapigenin were significantly lower. All tested extracts have shown spasmolytic effect in a dose-dependent manner on spontaneous contractions, acetylcholine- and potassium chloride-induced contractions; lyophilized extract obtained in maceration has shown significantly better spasmolytic activity. The mentioned extract has shown dosedependent spasmolytic effect in the model of contractions induced by barium chloride and calcium chloride, as well. Furthermore, lyophilized extracts were effective against Gram-positive bacteria and showed only moderate antifungal activity. Ultrasound-assisted extraction provided extracts with better ability of neutralization DPPH radicals, whereas maceration and ultrasound-assisted extraction approved to be better regarding beta-carotene bleaching assay. Due to spasmolytic, antimicrobial and antioxidant properties shown in the present study, polyphenols-rich T. serpyllum extract may be used as adjuvant in therapy of gastrointestinal disorders and infections caused by some of the susceptible pathogens.",
journal = "South African Journal of Botany",
title = "Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties",
pages = "283-274",
volume = "142",
doi = "10.1016/j.sajb.2021.06.028"
}

Jovanović, A., Petrović, P., Zdunic, G. M., Savikin, K. P., Kitic, D., Đorđević, V., Bugarski, B.,& Brankovic, S.. (2021). Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties. in South African Journal of Botany, 142, 274-283.
https://doi.org/10.1016/j.sajb.2021.06.028

Jovanović A, Petrović P, Zdunic GM, Savikin KP, Kitic D, Đorđević V, Bugarski B, Brankovic S. Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties. in South African Journal of Botany. 2021;142:274-283.
doi:10.1016/j.sajb.2021.06.028 .

Jovanović, Aleksandra, Petrović, Predrag, Zdunic, Gordana M., Savikin, Katarina P., Kitic, Dusanka, Đorđević, Verica, Bugarski, Branko, Brankovic, Suzana, "Influence of lyophilized Thymus serpyllum L. extracts on the gastrointestinal system: Spasmolytic, antimicrobial and antioxidant properties" in South African Journal of Botany, 142 (2021):274-283,
https://doi.org/10.1016/j.sajb.2021.06.028 . .

TY  - JOUR
AU  - Jovanovic, Milos
AU  - Cujic-Nikolic, Nada
AU  - Drinic, Zorica
AU  - Jankovic, Teodora
AU  - Markovic, Smilja
AU  - Petrović, Predrag
AU  - Savikin, Katarina
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4799
AB  - Willow gentian as a source of bitter compounds is traditionally used for digestive disorders. Gentiana root extract was spray dried using five different carriers (maltodextrin, whey protein, pectin, starch, gelatine) at various concentrations. Powders were characterized in terms of physical properties and encapsulation efficiency of bioactive compounds. The moisture content of all powders was between 1.78 and 3.46 %, and bulk density from 0.23 to 0.32 g/mL. Powders produced with maltodextrin and whey protein provided the highest yield (around 75 and 70 %, respectively) and the lowest hygroscopicity (6 and 7%, respectively). Gelatin and pectin provided powders with the highest encapsulation efficiency of total phenolic as well as the individual compounds. The stability of encapsulated bioactive compounds was studied after 6 months, and the most stable in all samples were gentiopicrin and sweroside with their content decreased by 10 % only. This study has shown that spray drying of gentian root extract produces powders with good physical properties and encapsulation of bioactives.
T2  - Industrial Crops and Products
T1  - Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds
VL  - 172
DO  - 10.1016/j.indcrop.2021.114044
ER  - 

@article{
author = "Jovanovic, Milos and Cujic-Nikolic, Nada and Drinic, Zorica and Jankovic, Teodora and Markovic, Smilja and Petrović, Predrag and Savikin, Katarina",
year = "2021",
abstract = "Willow gentian as a source of bitter compounds is traditionally used for digestive disorders. Gentiana root extract was spray dried using five different carriers (maltodextrin, whey protein, pectin, starch, gelatine) at various concentrations. Powders were characterized in terms of physical properties and encapsulation efficiency of bioactive compounds. The moisture content of all powders was between 1.78 and 3.46 %, and bulk density from 0.23 to 0.32 g/mL. Powders produced with maltodextrin and whey protein provided the highest yield (around 75 and 70 %, respectively) and the lowest hygroscopicity (6 and 7%, respectively). Gelatin and pectin provided powders with the highest encapsulation efficiency of total phenolic as well as the individual compounds. The stability of encapsulated bioactive compounds was studied after 6 months, and the most stable in all samples were gentiopicrin and sweroside with their content decreased by 10 % only. This study has shown that spray drying of gentian root extract produces powders with good physical properties and encapsulation of bioactives.",
journal = "Industrial Crops and Products",
title = "Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds",
volume = "172",
doi = "10.1016/j.indcrop.2021.114044"
}

Jovanovic, M., Cujic-Nikolic, N., Drinic, Z., Jankovic, T., Markovic, S., Petrović, P.,& Savikin, K.. (2021). Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds. in Industrial Crops and Products, 172.
https://doi.org/10.1016/j.indcrop.2021.114044

Jovanovic M, Cujic-Nikolic N, Drinic Z, Jankovic T, Markovic S, Petrović P, Savikin K. Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds. in Industrial Crops and Products. 2021;172.
doi:10.1016/j.indcrop.2021.114044 .

Jovanovic, Milos, Cujic-Nikolic, Nada, Drinic, Zorica, Jankovic, Teodora, Markovic, Smilja, Petrović, Predrag, Savikin, Katarina, "Spray drying of Gentiana asclepiadea L. root extract: Successful encapsulation into powders with preserved stability of bioactive compounds" in Industrial Crops and Products, 172 (2021),
https://doi.org/10.1016/j.indcrop.2021.114044 . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana D.
AU  - Petrović, Predrag
AU  - Pravilović, Radoslava
AU  - Đorđević, Verica B.
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5997
AB  - In traditional medicine Thymus serpyllum L. (wild thyme) herb represents a part of a large number of herbal medicinal formulations such as syrups, tinctures, infusions, teas, and decoctions. In recent years, there has been a growing interest in testing the biological properties of wild thyme, because the plant is a high-quality raw material, rich in essential oil and pharmacologically active polyphenolic compounds, which can be included in various formulations in the pharmaceutical, cosmetic, food, and chemical industries. Wild thyme extracts and essential oil have shown significant nitric oxide, ABTS and DPPH radicals scavenging potential, ferrous ion chelating property, reducing activity and ability to inhibit peroxidation of deoxyribonucleic acid (DNA), proteins and lipids. Wild thyme extracts exerted antibacterial activity against Bacillus cereus, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enterica, Yersinia enterocolitica, as well as Lactic acid bacteria. Wild thyme essential oil has shown antibacterial potential against E. coli, L. monocytogenes, P. aeruginosa, S. enteritidis, S. aureus, Streptococcus salivariu, S. mutans, S. sanguinis, S. pyogenes, E. feacalis, B. cereus, B. subtilis, B. pumilis, Lactobacillus acidophilus, Proteus mirabilis, Klebsiella pneumoniae, Salmonella choleraesuis, and Salmonella poona. T. serpyllum extract possesses antifungal activity against Fusarium solani, F. moniliforme, Aspergillus flavus, Microsporum canis, Alternaria species, Candida albicans, and C. glabarata. The essential oil efficiently inhibited the growth of C. albicans, C. glabrata and Aspergillus fungi. Depending on the concentration, wild thyme extract significantly relaxed spontaneous contractions, as well as acetylcholine-, potassium chloride-, barium chlorideand calcium chloride-induced contractions of the isolated rat ileum. The extract significantly reduced the expression of the inducible enzyme cyclooxygenase-2 and inhibited acetylcholinesterase, myeloperoxidase and α-glucosidase activity. T. serpyllum extract has shown cytotoxic activity on human breast cancer cell lines, while essential oil has shown the antitumor potential in human cell lung cancer, colon, cervical, hepatocellular, prostate, and breast adeno-carcinoma.
PB  - University of East Sarajevo, Faculty of Technology
T2  - Journal of Engineering & Processing Management
T1  - Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review
EP  - 41
IS  - 2
SP  - 32
VL  - 13
DO  - 10.7251/JEPM2102032J
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Balanč, Bojana D. and Petrović, Predrag and Pravilović, Radoslava and Đorđević, Verica B.",
year = "2021",
abstract = "In traditional medicine Thymus serpyllum L. (wild thyme) herb represents a part of a large number of herbal medicinal formulations such as syrups, tinctures, infusions, teas, and decoctions. In recent years, there has been a growing interest in testing the biological properties of wild thyme, because the plant is a high-quality raw material, rich in essential oil and pharmacologically active polyphenolic compounds, which can be included in various formulations in the pharmaceutical, cosmetic, food, and chemical industries. Wild thyme extracts and essential oil have shown significant nitric oxide, ABTS and DPPH radicals scavenging potential, ferrous ion chelating property, reducing activity and ability to inhibit peroxidation of deoxyribonucleic acid (DNA), proteins and lipids. Wild thyme extracts exerted antibacterial activity against Bacillus cereus, Enterococcus faecalis, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella enterica, Yersinia enterocolitica, as well as Lactic acid bacteria. Wild thyme essential oil has shown antibacterial potential against E. coli, L. monocytogenes, P. aeruginosa, S. enteritidis, S. aureus, Streptococcus salivariu, S. mutans, S. sanguinis, S. pyogenes, E. feacalis, B. cereus, B. subtilis, B. pumilis, Lactobacillus acidophilus, Proteus mirabilis, Klebsiella pneumoniae, Salmonella choleraesuis, and Salmonella poona. T. serpyllum extract possesses antifungal activity against Fusarium solani, F. moniliforme, Aspergillus flavus, Microsporum canis, Alternaria species, Candida albicans, and C. glabarata. The essential oil efficiently inhibited the growth of C. albicans, C. glabrata and Aspergillus fungi. Depending on the concentration, wild thyme extract significantly relaxed spontaneous contractions, as well as acetylcholine-, potassium chloride-, barium chlorideand calcium chloride-induced contractions of the isolated rat ileum. The extract significantly reduced the expression of the inducible enzyme cyclooxygenase-2 and inhibited acetylcholinesterase, myeloperoxidase and α-glucosidase activity. T. serpyllum extract has shown cytotoxic activity on human breast cancer cell lines, while essential oil has shown the antitumor potential in human cell lung cancer, colon, cervical, hepatocellular, prostate, and breast adeno-carcinoma.",
publisher = "University of East Sarajevo, Faculty of Technology",
journal = "Journal of Engineering & Processing Management",
title = "Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review",
pages = "41-32",
number = "2",
volume = "13",
doi = "10.7251/JEPM2102032J"
}

Jovanović, A. A., Balanč, B. D., Petrović, P., Pravilović, R.,& Đorđević, V. B.. (2021). Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review. in Journal of Engineering & Processing Management
University of East Sarajevo, Faculty of Technology., 13(2), 32-41.
https://doi.org/10.7251/JEPM2102032J

Jovanović AA, Balanč BD, Petrović P, Pravilović R, Đorđević VB. Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review. in Journal of Engineering & Processing Management. 2021;13(2):32-41.
doi:10.7251/JEPM2102032J .

Jovanović, Aleksandra A., Balanč, Bojana D., Petrović, Predrag, Pravilović, Radoslava, Đorđević, Verica B., "Pharmacological potential of Thymus serpyllum L. (wild thyme) extracts and essential oil: A review" in Journal of Engineering & Processing Management, 13, no. 2 (2021):32-41,
https://doi.org/10.7251/JEPM2102032J . .

TY  - JOUR
AU  - Jovanović, Aleksandra A.
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6409
AB  - Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries.
AB  - Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.
PB  - Serbian Nutrition Society, Belgrade
T2  - Hrana i ishrana
T1  - Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques
EP  - 20
IS  - 2
SP  - 15
VL  - 62
DO  - 10.5937/hraIsh2102015J
ER  - 

@article{
author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as plant secondary metabolites, achieve strong antioxidant capacity by scavenging free radicals, chelating metals, and interacting with lipid membranes, proteins and nucleic acids. The aim of the present study was the examination of the applied extraction conditions (particle size of plant material, solvent-to-solid ratio, solvent type, and extraction time) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the free radicals' scavenging capacity of Thymus serpyllum polyphenol extracts, determined in ABTS and DPPH methods. According to the results, smaller particle size of herbal drug resulted with better antioxidant potential. However, this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. Furthermore, the increase in solvent-to-solid ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant activity regardless of the type of extraction technique, whereas the scavenging activity of ethanol extracts was higher in comparison to aqueous extracts. In terms of extraction time for achieving the highest free radicals' scavenging capacity, 30 min can be recommended for maceration and heat-assisted extraction, and 5 min for processing by ultrasonic probe. In neutralization of ABTS radicals, there were no statistically significant differences between antioxidant capacity of T. serpyllum extracts obtained by using different extraction techniques, whereas in DPPH scavenging activity, the extracts obtained in ultrasound-assisted extraction have shown statistically significantly higher activity. Due to the free radicals' scavenging properties shown in the present study, T. serpyllum extracts may be used in the formulations in food, pharmaceutical, cosmetic, and chemical industries., Polifenoli, kao biljni sekundarni metaboliti, poseduju
snažan antioksidativni kapacitet za neutralizaciju slobodnih radikala, heliranje metala i interakciju sa lipidnim membranama, proteinima i nukleinskim kiselinama. Cilj prikazane studije je ispitivanje primenjenih uslova ekstrakcije
(veličine čestica biljnog materijala, odnos rastvarač:biljna
droga, vrsta rastvarača i vreme ekstrakcije) i metode
ekstrakcije (maceracija, ekstrakcija na povišenoj temperaturi i ekstrakcija ultrazvučnim talasima) na sposobnost
neutralizacije slobodnih radikala od strane polifenolnih ekstrakata biljke Thymus serpyllum, ispitanu primenom ABTS i
DPPH metoda. Prema dobijenim rezultatima, manje čestice biljnog materijala daju ekstrakte sa boljim antioksidativnim
potencijalom. Međutim, ovaj efekat je statistički značajan za ekstrakte dobijene na povišenoj temperaturi i ultrazvučnim
talasima prema ABTS testu, dok je u DPPH testu efekat bio značajan samo za esktrakte dobijene maceracijom i na
povišenoj temperaturi. Povećanje odnosa rastvarač:biljna droga od 10:1 do 30:1 dovodi do povećanja antioksidativne
aktivnosti ekstrakata bez obzira na primenjenu tehniku ekstrakcije, dok etanolni ekstrakti pokazuju bolji potencijal u neutralizaciji slobodnih radikala u odnosu na vodene ekstrakte. Pri ispitivanju vremena ekstrakcije koje je neophodno da se
dostigne najveći antioksidativni kapacitet ekstrakta, pokazano je da je 30 min optimalno vreme za maceraciju i ekstrakciju na povišenoj temperaturi, dok je za ekstrakciju ultrazvučnom sondom potrebno 5 min. Pri neutralizaciji ABTS radikala
nije bilo statistički značajne razlike između T. serpyllum ekstrakata dobijenim različitim tehnikama, dok su u DPPH testu
ekstrakti dobijeni primenom ultrazvučne sonde pokazali statistički značajno veću aktivnost. Zahvaljujući prikazanim svojstvima u neutralizaciji slobodnih radikala, ekstrakti biljke T. serpyllum mogu se koristiti u formulacijama u prehrambenoj,
farmaceutskoj, kozmetičkoj i hemijskoj industriji.",
publisher = "Serbian Nutrition Society, Belgrade",
journal = "Hrana i ishrana",
title = "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques",
pages = "20-15",
number = "2",
volume = "62",
doi = "10.5937/hraIsh2102015J"
}

Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana
Serbian Nutrition Society, Belgrade., 62(2), 15-20.
https://doi.org/10.5937/hraIsh2102015J

Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Bugarski B. Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques. in Hrana i ishrana. 2021;62(2):15-20.
doi:10.5937/hraIsh2102015J .

Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "Free radicals' scavenging capacity of Thymus serpyllum L. extracts depending on applied extraction conditions and extraction techniques" in Hrana i ishrana, 62, no. 2 (2021):15-20,
https://doi.org/10.5937/hraIsh2102015J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Zdunić, Gordana
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6410
AB  - Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Thymus serpyllum extract loaded liposomes produced by proliposome method
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6410
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Petrović, Predrag and Đorđević, Verica and Šavikin, Katarina and Zdunić, Gordana and Bugarski, Branko",
year = "2021",
abstract = "Polyphenols, as secondary metabolites from plants, are used as food complements or ingredients
within the pharmaceutical or cosmetic formulations. However, their use is rather limited, due to
their low bioavailability, integrity, permeability, and solubility. Namely, polyphenols’ sensitivity to
environmental factors during food processing, distribution, or storage, or in the gastrointestinal
tract, also limit their activity and the potential health benefits. Thus, the encapsulation of
polyphenol extracts represents an appropriate way to overcome the mentioned disadvantages.
Thymus serpyllum L. ethanol extract obtained in heat-assisted extraction (80°C), rich in polyphenol
compounds, was encapsulated into liposomes produced using phospholipids and proliposome
method. Total polyphenol content in extract and encapsulation efficiency were determined using the
Folin-Ciocalteu procedure. Particle size, polydispersity index, and zeta potential of empty and
extract loaded liposomes were measured during 28 days using photon correlation spectroscopy.
Total polyphenol content of the extract was amounted to 2.08±0.14 mg of gallic acid
equivalents/mL, whereas encapsulation efficiency was 89.4±0.8%. During the 28-days stability
study, the particle size of empty liposomes varied between 420.6±4.3 and 581.6±3.4 nm with
polydispersity index from 0.109±0.067 to 0.295±0.009, while the size of extract loaded liposomes
was between 278.7±1.5 and 456.4±9.3 nm with polydispersity index 0.179±0.094 to 0.284±0.005.
Zeta potential of empty liposomes varied from -17.1±0.2 to -27.3±0.5 mV, whereas the zeta
potential of extract loaded liposomes was between -13.6±0.3 and -25.4±1.4 mV. The aim of this
study is to provide evidence for food manufacturers and food scientists to make broader use of T.
serpyllum loaded liposomes that can add value and improve the quality of existing food,
pharmaceutical and cosmetic products.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Thymus serpyllum extract loaded liposomes produced by proliposome method",
pages = "144",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6410"
}

Jovanović, A. A., Balanč, B., Petrović, P., Đorđević, V., Šavikin, K., Zdunić, G.,& Bugarski, B.. (2021). Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference
University of Belgrade..
https://hdl.handle.net/21.15107/rcub_technorep_6410

Jovanović AA, Balanč B, Petrović P, Đorđević V, Šavikin K, Zdunić G, Bugarski B. Thymus serpyllum extract loaded liposomes produced by proliposome method. in 2nd International UNIfood Conference. 2021;:null-144.
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

Jovanović, Aleksandra A., Balanč, Bojana, Petrović, Predrag, Đorđević, Verica, Šavikin, Katarina, Zdunić, Gordana, Bugarski, Branko, "Thymus serpyllum extract loaded liposomes produced by proliposome method" in 2nd International UNIfood Conference (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6410 .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Petrović, Predrag
AU  - Đorđević, Verica
AU  - Mijin, Dušan
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6443
AB  - Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.
PB  - Faculty of Technology, Zvornik
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
T1  - The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme
EP  - 143
SP  - 137
DO  - 10.7251/EEMEN2101137J
ER  - 

@conference{
author = "Jovanović, Aleksandra and Petrović, Predrag and Đorđević, Verica and Mijin, Dušan and Zdunić, Gordana and Šavikin, Katarina and Bugarski, Branko",
year = "2021",
abstract = "Aromatic plants are commercially important and find wide use in food and pharmaceutical
industry, because of health benefits related with their antioxidant, antimicrobial, antiinflammatory, cardioprotective, cytotoxic, expectorant, anti-spasmodic and stimulant effects.
Most of phytochemicals are impossible to synthesize, therefore the best option is their extraction
from natural sources. Microwave-assisted extraction offers a rapid delivery of energy to a total
volume of solvent and solid plant material, improving the recovery of polyphenols. In the study,
wild thyme herb was evaluated as a source of polyphenols, which were extracted by the
application of microwaves instead of the traditional extraction techniques. The objective of the
present study was optimization of microwave-assisted extraction process through varying
irradiation time (5-180 s), temperature (60-200°C), particle size (0.3, 0.7 and 1.5 mm), solventto-solid ratio (10:1, 20:1, 30:1 and 40:1) and ethanol concentration in the extraction medium (0-
60%). Extraction efficiency was expressed via total polyphenol content (Folin-Ciocalteu method)
and antioxidant activity (ABTS radical scavenging method). The statistical analysis (one-way
ANOVA and Duncan's post-hoc test) has revealed that irradiation time above 15 s and particle
size have not shown statistically significant influence on polyphenol yield, while there were
significant differences between extracts obtained by various temperatures and solvent-to-solid
ratios. Although the highest polyphenol yield has achieved by using 50% of ethanol, there was
no statistically significant difference between ethanol concentration in the range from 30% to
50%. The highest polyphenol yield and antioxidant activity were achived in the extract with
particle size of 0.3 mm, 30:1 ratio and 50% ethanol, after 15 s, at 200°C (45.11 mg GAE/L and
27.22 µmol/L Trolox). According to the presented results, microwave-assisted extraction may be
used as an efficient and time saving alternative to traditional extraction techniques for extraction
of bioactive polyphenolic compounds from wild thyme.",
publisher = "Faculty of Technology, Zvornik",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM",
title = "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme",
pages = "143-137",
doi = "10.7251/EEMEN2101137J"
}

Jovanović, A., Petrović, P., Đorđević, V., Mijin, D., Zdunić, G., Šavikin, K.,& Bugarski, B.. (2021). The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM
Faculty of Technology, Zvornik., 137-143.
https://doi.org/10.7251/EEMEN2101137J

Jovanović A, Petrović P, Đorđević V, Mijin D, Zdunić G, Šavikin K, Bugarski B. The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme. in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM. 2021;:137-143.
doi:10.7251/EEMEN2101137J .

Jovanović, Aleksandra, Petrović, Predrag, Đorđević, Verica, Mijin, Dušan, Zdunić, Gordana, Šavikin, Katarina, Bugarski, Branko, "The influence of different microwave-assisted extraction conditions on polyphenol yield and antioxidant activity of wild thyme" in VII International Congress “Engineering, Environment and Materials in Process Industry“ - EEM (2021):137-143,
https://doi.org/10.7251/EEMEN2101137J . .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Trajković, Milica
AU  - Đorđević, Verica
AU  - Šavikin, Katarina
AU  - Živković, Jelena
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6411
AB  - Rosa canina pseudo-fruit is rich in bioactive substances, such are carotenoids, ascorbic acid,
polyphenols, fatty acids and minerals. Because of its diuretic, anti-inflammatory, anti-allergic and
antioxidant properties it is frequently employed in traditional medicine. However, in order to
protect mentioned bioactive compounds and to improve its bioavailability and controlled release,
numerous encapsulation methods have been developed. Thus, R. canina ethanol extract obtained
using percolation at ambient temperature (25°C), was encapsulated into Lipoid G liposomes
produced in thin film procedure. Total polyphenol and flavonoid contents, as well as antioxidant
potential of the extract and extract loaded liposomes were determined. Encapsulation efficiency,
particle size, polydispersity index and zeta potential of the liposomes with extract were monitoring
during 30 days. Total polyphenol and flavonoid contents of the extract were 103.8±1.9 mg of gallic
acid equivalents/g of the extract and 64.2±2.4 mg of catechin equivalents/g of the extract,
respectively; antioxidant potential of the extract was 15.3±2.1 mg of Trolox equivalents/g of the
extract (ABTS assay) and 1.07±0.01 mg/mL of the extract (DPPH assay). Total polyphenol and
flavonoid contents of the extract loaded liposomes were 0.316±0.023 mg of gallic acid
equivalents/mg of lipids and 0.219±0.009 mg of catechin equivalents/mg of lipids, respectively;
antioxidant potential of the sample was 0.134±0.004 mg of Trolox equivalents/mg of lipids (ABTS
assay) and 270.6±10.5 mg/mL of liposomal suspension (DPPH assay). Encapsulation efficiency
was the same during monitoring period and it was amounted 46.6±3.4%. Particle size and
polydispersity index of the liposomes with extract were increased from 618.2±10.9 to 1698.0±104.2
nm and from 0.441±0.007 to 0.589±0.011, respectively, during 30 days. On the other hand, zeta
potential of the extract loaded liposomes was decreased from -10.24±0.95 to -7.21±0.77 mV. R.
canina extract loaded liposomes developed in this study have potential to be used in food,
pharmacological and cosmetic industries due to beneficial health effects of R. canina active
compounds encapsulated into liposomes.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Encapsulation of Rosa canina extract in liposomes produced by thin film method
EP  - 176
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6411
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Trajković, Milica and Đorđević, Verica and Šavikin, Katarina and Živković, Jelena and Bugarski, Branko",
year = "2021",
abstract = "Rosa canina pseudo-fruit is rich in bioactive substances, such are carotenoids, ascorbic acid,
polyphenols, fatty acids and minerals. Because of its diuretic, anti-inflammatory, anti-allergic and
antioxidant properties it is frequently employed in traditional medicine. However, in order to
protect mentioned bioactive compounds and to improve its bioavailability and controlled release,
numerous encapsulation methods have been developed. Thus, R. canina ethanol extract obtained
using percolation at ambient temperature (25°C), was encapsulated into Lipoid G liposomes
produced in thin film procedure. Total polyphenol and flavonoid contents, as well as antioxidant
potential of the extract and extract loaded liposomes were determined. Encapsulation efficiency,
particle size, polydispersity index and zeta potential of the liposomes with extract were monitoring
during 30 days. Total polyphenol and flavonoid contents of the extract were 103.8±1.9 mg of gallic
acid equivalents/g of the extract and 64.2±2.4 mg of catechin equivalents/g of the extract,
respectively; antioxidant potential of the extract was 15.3±2.1 mg of Trolox equivalents/g of the
extract (ABTS assay) and 1.07±0.01 mg/mL of the extract (DPPH assay). Total polyphenol and
flavonoid contents of the extract loaded liposomes were 0.316±0.023 mg of gallic acid
equivalents/mg of lipids and 0.219±0.009 mg of catechin equivalents/mg of lipids, respectively;
antioxidant potential of the sample was 0.134±0.004 mg of Trolox equivalents/mg of lipids (ABTS
assay) and 270.6±10.5 mg/mL of liposomal suspension (DPPH assay). Encapsulation efficiency
was the same during monitoring period and it was amounted 46.6±3.4%. Particle size and
polydispersity index of the liposomes with extract were increased from 618.2±10.9 to 1698.0±104.2
nm and from 0.441±0.007 to 0.589±0.011, respectively, during 30 days. On the other hand, zeta
potential of the extract loaded liposomes was decreased from -10.24±0.95 to -7.21±0.77 mV. R.
canina extract loaded liposomes developed in this study have potential to be used in food,
pharmacological and cosmetic industries due to beneficial health effects of R. canina active
compounds encapsulated into liposomes.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Encapsulation of Rosa canina extract in liposomes produced by thin film method",
pages = "176",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6411"
}

Jovanović, A. A., Balanč, B., Trajković, M., Đorđević, V., Šavikin, K., Živković, J.,& Bugarski, B.. (2021). Encapsulation of Rosa canina extract in liposomes produced by thin film method. in 2nd International UNIfood Conference
University of Belgrade..
https://hdl.handle.net/21.15107/rcub_technorep_6411

Jovanović AA, Balanč B, Trajković M, Đorđević V, Šavikin K, Živković J, Bugarski B. Encapsulation of Rosa canina extract in liposomes produced by thin film method. in 2nd International UNIfood Conference. 2021;:null-176.
https://hdl.handle.net/21.15107/rcub_technorep_6411 .

Jovanović, Aleksandra A., Balanč, Bojana, Trajković, Milica, Đorđević, Verica, Šavikin, Katarina, Živković, Jelena, Bugarski, Branko, "Encapsulation of Rosa canina extract in liposomes produced by thin film method" in 2nd International UNIfood Conference (2021),
https://hdl.handle.net/21.15107/rcub_technorep_6411 .

TY  - CONF
AU  - Jovanović, Aleksandra A.
AU  - Balanč, Bojana
AU  - Šavikin, Katarina
AU  - Živković, Jelena
AU  - Đorđević, Verica
AU  - Bugarski, Branko
PY  - 2021
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6442
AB  - Rosa canina L. (Rosaceae) is frequently employed in traditional medicine, due to its diuretic,
anti-inflammatory, anti-allergic, antioxidant and analgesic effects. It is rich in various bioactive
compounds, including polyphenols, carotenoids, ascorbic acid, mineral elements and fatty acids.
Microencapsulation tehnique is a promising tool for improving R. canina polyphenols'
functionality, stability and bioavailability. Еlectrostatic extrusion process was carried out to
obtain calcium-alginate beads with encapsulated R. canina extract. In pure extract, total
polyphenol content was 103.8±1.9 mg GAE/g, whereas the concentration of flavonoids was
64.2±2.40 mg CE/g. Furthermore, polyphenol and flavonoid contents in extract loaded hydrogel
beads were 4.68±0.12 mg GAE/g and 2.93±0.23 mg CE/g, respectively. Thus, determined
encapsulation efficiency was 45.04±1.15%. In ABTS test, antioxidant activity of pure extract and
extract loaded hydrogel beads was 15.30±2.10 and 2.93±0.23 mg Trolox/g, respectively. In
DPPH test, IC50 of pure extract and extract loaded hydrogel beads was 1.07±0.01 and
29.32±2.15 mg/mL, respectively. The size of the produced R. canina extract encapsulated
hydrogel particles was 550.30±34.56 µm. In distilled water as a medium, most of polyphenols
were released relatively rapidly from alginate beads (in the first 20 min), and the maximum
release plateau was reached at 60 min. This study demonstrates the potential of using alginate
for encapsulation of R. canina polyphenols, with the aim to improve their functionality,
bioavailability and stability in food, cosmetic and pharmaceutical products.
PB  - Faculty of Technology, Zvornik
C3  - VII International Congress “Engineering, Environment and Materials in Process Industry“
T1  - Alginate-based encapsulation of polyphenols from Rosa canina L. extract
EP  - 136
SP  - 131
DO  - 10.7251/EEMEN2101131J
ER  - 

@conference{
author = "Jovanović, Aleksandra A. and Balanč, Bojana and Šavikin, Katarina and Živković, Jelena and Đorđević, Verica and Bugarski, Branko",
year = "2021",
abstract = "Rosa canina L. (Rosaceae) is frequently employed in traditional medicine, due to its diuretic,
anti-inflammatory, anti-allergic, antioxidant and analgesic effects. It is rich in various bioactive
compounds, including polyphenols, carotenoids, ascorbic acid, mineral elements and fatty acids.
Microencapsulation tehnique is a promising tool for improving R. canina polyphenols'
functionality, stability and bioavailability. Еlectrostatic extrusion process was carried out to
obtain calcium-alginate beads with encapsulated R. canina extract. In pure extract, total
polyphenol content was 103.8±1.9 mg GAE/g, whereas the concentration of flavonoids was
64.2±2.40 mg CE/g. Furthermore, polyphenol and flavonoid contents in extract loaded hydrogel
beads were 4.68±0.12 mg GAE/g and 2.93±0.23 mg CE/g, respectively. Thus, determined
encapsulation efficiency was 45.04±1.15%. In ABTS test, antioxidant activity of pure extract and
extract loaded hydrogel beads was 15.30±2.10 and 2.93±0.23 mg Trolox/g, respectively. In
DPPH test, IC50 of pure extract and extract loaded hydrogel beads was 1.07±0.01 and
29.32±2.15 mg/mL, respectively. The size of the produced R. canina extract encapsulated
hydrogel particles was 550.30±34.56 µm. In distilled water as a medium, most of polyphenols
were released relatively rapidly from alginate beads (in the first 20 min), and the maximum
release plateau was reached at 60 min. This study demonstrates the potential of using alginate
for encapsulation of R. canina polyphenols, with the aim to improve their functionality,
bioavailability and stability in food, cosmetic and pharmaceutical products.",
publisher = "Faculty of Technology, Zvornik",
journal = "VII International Congress “Engineering, Environment and Materials in Process Industry“",
title = "Alginate-based encapsulation of polyphenols from Rosa canina L. extract",
pages = "136-131",
doi = "10.7251/EEMEN2101131J"
}

Jovanović, A. A., Balanč, B., Šavikin, K., Živković, J., Đorđević, V.,& Bugarski, B.. (2021). Alginate-based encapsulation of polyphenols from Rosa canina L. extract. in VII International Congress “Engineering, Environment and Materials in Process Industry“
Faculty of Technology, Zvornik., 131-136.
https://doi.org/10.7251/EEMEN2101131J

Jovanović AA, Balanč B, Šavikin K, Živković J, Đorđević V, Bugarski B. Alginate-based encapsulation of polyphenols from Rosa canina L. extract. in VII International Congress “Engineering, Environment and Materials in Process Industry“. 2021;:131-136.
doi:10.7251/EEMEN2101131J .

Jovanović, Aleksandra A., Balanč, Bojana, Šavikin, Katarina, Živković, Jelena, Đorđević, Verica, Bugarski, Branko, "Alginate-based encapsulation of polyphenols from Rosa canina L. extract" in VII International Congress “Engineering, Environment and Materials in Process Industry“ (2021):131-136,
https://doi.org/10.7251/EEMEN2101131J . .