TY  - CONF
AU  - Marković, Milica
AU  - Markov, Siniša
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Vukašinović, Maja
AU  - Pejin, Jelena
AU  - Joković, Nataša
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6303
AB  - Triticale is a very applicable crop for bioethanol production, and also provides a stillage that can be used as a feed. The main aim of this research was to investigate the possibility of transformation of unused stillage carbohydrate after bioethanol prooduction into the lactis acid fermentation, in order to produce a natural feed preservative. In this study Lactobacillus fermentum Pl-1 was used. Different pre-treatment were done during the preparation of bioethanol fermentation (by triticale autoamylolytic enzymes, technical enzymes and ultrasound), and thus 4 different samples of stillage were prepared. CaCO3 was added in one part of the samples. Results indicated a good conversion factor of glucose into the lactic acid, up to 1.55 after 72 hours of the treatment. The sample pre-treated only by triticale autoamylolytic enzymes had a small part of carbohydrtes utilised during the stillage preparation. As a result, there was more glucose available for lactic acid fermentation. Added CaCo3 probably improved the protection of Lactobacillus fermentum PL-1 cells, but it was not possible to see a real glucose conversion factor due to Ca-lactate formation. Therefore, the stillage produced after the bioethanol production is a very good medium for lactic acid production
PB  - Beograd : Tehnološko-metalurški fakultet Univerziteta
C3  - Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine
T1  - Primena džibre tritikalea za proizvodnju mlečne kiseline
T1  - Triticale stillage appliance for lactic acid production
EP  - 33
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6303
ER  - 

@conference{
author = "Marković, Milica and Markov, Siniša and Pejin, Dušanka and Mojović, Ljiljana and Vukašinović, Maja and Pejin, Jelena and Joković, Nataša",
year = "2010",
abstract = "Triticale is a very applicable crop for bioethanol production, and also provides a stillage that can be used as a feed. The main aim of this research was to investigate the possibility of transformation of unused stillage carbohydrate after bioethanol prooduction into the lactis acid fermentation, in order to produce a natural feed preservative. In this study Lactobacillus fermentum Pl-1 was used. Different pre-treatment were done during the preparation of bioethanol fermentation (by triticale autoamylolytic enzymes, technical enzymes and ultrasound), and thus 4 different samples of stillage were prepared. CaCO3 was added in one part of the samples. Results indicated a good conversion factor of glucose into the lactic acid, up to 1.55 after 72 hours of the treatment. The sample pre-treated only by triticale autoamylolytic enzymes had a small part of carbohydrtes utilised during the stillage preparation. As a result, there was more glucose available for lactic acid fermentation. Added CaCo3 probably improved the protection of Lactobacillus fermentum PL-1 cells, but it was not possible to see a real glucose conversion factor due to Ca-lactate formation. Therefore, the stillage produced after the bioethanol production is a very good medium for lactic acid production",
publisher = "Beograd : Tehnološko-metalurški fakultet Univerziteta",
journal = "Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine",
title = "Primena džibre tritikalea za proizvodnju mlečne kiseline, Triticale stillage appliance for lactic acid production",
pages = "33-32",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6303"
}

Marković, M., Markov, S., Pejin, D., Mojović, L., Vukašinović, M., Pejin, J.,& Joković, N.. (2010). Primena džibre tritikalea za proizvodnju mlečne kiseline. in Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine
Beograd : Tehnološko-metalurški fakultet Univerziteta., 32-33.
https://hdl.handle.net/21.15107/rcub_technorep_6303

Marković M, Markov S, Pejin D, Mojović L, Vukašinović M, Pejin J, Joković N. Primena džibre tritikalea za proizvodnju mlečne kiseline. in Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine. 2010;:32-33.
https://hdl.handle.net/21.15107/rcub_technorep_6303 .

Marković, Milica, Markov, Siniša, Pejin, Dušanka, Mojović, Ljiljana, Vukašinović, Maja, Pejin, Jelena, Joković, Nataša, "Primena džibre tritikalea za proizvodnju mlečne kiseline" in Knjiga izvoda radova / Nacionalna konferencija sa međunarodnim učešćem Biotehnologija za održivi razvoj, Beograd, 24-26.11. 2010. godine (2010):32-33,
https://hdl.handle.net/21.15107/rcub_technorep_6303 .

TY  - CONF
AU  - Vukašinović-Sekulić, Maja
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Nikolić, Svetlana
AU  - Djukić, Aleksandra
AU  - Marković, Milica
AU  - Markov, Siniša
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6210
AB  - Bioethanol produced by fermentation of biomass, such as sugar, starch, or lignocellulosic materials, is one of the most important renewable energy resources today. Beside the bioethanol, considerable amounts of by-products such as carbon dioxide, solid and liquid stillage are formed during the fermentation. Their further utilization could significantly increase the economy of bioethanol production. It is estimated that per 1hl of the produced bioethanol, approximately 13 hl of liquid thin stillage is formed. It contains high concentrations of organic and inorganic substances and must be pretreated before its disposal to water flows. In addition to recirculation of thin stillage and its reuse, production of soil fertilizers and various types of animal feed, the thin stillage can be used as a substrate for anaerobic fermentation for the production of butanol or especially lactic acid. Lactic acid has versatile application not only in food, pharmaceutical, leather and textile industries, but also as a chemical feedstock for the production of base chemicals (propylene glycol, propylene oxide, acrylic acid, acrylate esters, lactate ester and etc) or for the preparation of biodegradable polymers (poly lactic acid), medical sutures, and green solvents. The aim of this work was to select appropriate species for lactic acid fermentation of the corn thin stillage which is an inexpensive renewable substrate.
PB  - Технолошко-металуршки факултет, Београд
C3  - Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem
T1  - Selection of strains from Lactobacillus sp. for lactic acid fermentation of thin stillage
EP  - 41
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6210
ER  - 

@conference{
author = "Vukašinović-Sekulić, Maja and Mojović, Ljiljana and Rakin, Marica and Nikolić, Svetlana and Djukić, Aleksandra and Marković, Milica and Markov, Siniša",
year = "2010",
abstract = "Bioethanol produced by fermentation of biomass, such as sugar, starch, or lignocellulosic materials, is one of the most important renewable energy resources today. Beside the bioethanol, considerable amounts of by-products such as carbon dioxide, solid and liquid stillage are formed during the fermentation. Their further utilization could significantly increase the economy of bioethanol production. It is estimated that per 1hl of the produced bioethanol, approximately 13 hl of liquid thin stillage is formed. It contains high concentrations of organic and inorganic substances and must be pretreated before its disposal to water flows. In addition to recirculation of thin stillage and its reuse, production of soil fertilizers and various types of animal feed, the thin stillage can be used as a substrate for anaerobic fermentation for the production of butanol or especially lactic acid. Lactic acid has versatile application not only in food, pharmaceutical, leather and textile industries, but also as a chemical feedstock for the production of base chemicals (propylene glycol, propylene oxide, acrylic acid, acrylate esters, lactate ester and etc) or for the preparation of biodegradable polymers (poly lactic acid), medical sutures, and green solvents. The aim of this work was to select appropriate species for lactic acid fermentation of the corn thin stillage which is an inexpensive renewable substrate.",
publisher = "Технолошко-металуршки факултет, Београд",
journal = "Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem",
title = "Selection of strains from Lactobacillus sp. for lactic acid fermentation of thin stillage",
pages = "41-40",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6210"
}

Vukašinović-Sekulić, M., Mojović, L., Rakin, M., Nikolić, S., Djukić, A., Marković, M.,& Markov, S.. (2010). Selection of strains from Lactobacillus sp. for lactic acid fermentation of thin stillage. in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem
Технолошко-металуршки факултет, Београд., 40-41.
https://hdl.handle.net/21.15107/rcub_technorep_6210

Vukašinović-Sekulić M, Mojović L, Rakin M, Nikolić S, Djukić A, Marković M, Markov S. Selection of strains from Lactobacillus sp. for lactic acid fermentation of thin stillage. in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem. 2010;:40-41.
https://hdl.handle.net/21.15107/rcub_technorep_6210 .

Vukašinović-Sekulić, Maja, Mojović, Ljiljana, Rakin, Marica, Nikolić, Svetlana, Djukić, Aleksandra, Marković, Milica, Markov, Siniša, "Selection of strains from Lactobacillus sp. for lactic acid fermentation of thin stillage" in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem (2010):40-41,
https://hdl.handle.net/21.15107/rcub_technorep_6210 .

TY  - CONF
AU  - Nikolić, Svetlana
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Dušanka
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Jelena
AU  - Djukić, Aleksandra
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6304
AB  - The lactic acid production from corn thin stillage using Lactobacillus rhamnosus ATCC 7469 in a batch system was studied. The fermentation temperature was optimized taking into account lactic acid production and glucose consumption as well as other significant process parameters (such as lactic acid yield and volumetric productivity). Also, the effect of pH control on lactic acid production by addition of 1% (w/w) CaCO3 and 10M NaOH was investigated. Based on the obtained results, an optimal fermentation temperature of 41 0C was selected. In this case, lactic acid yield of 0.70 g/g and utilized glucose of 72.55% were achieved. The addition of 10 M NaOH after 72h of fermentation at optimal temperature improved substrate (glucose) utilization for 63.70% compared to the control sample.
PB  - Технолошко-металуршки факултет, Београд
C3  - Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem
T1  - Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469
EP  - 51
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_technorep_6304
ER  - 

@conference{
author = "Nikolić, Svetlana and Vukašinović-Sekulić, Maja and Pejin, Dušanka and Mojović, Ljiljana and Rakin, Marica and Pejin, Jelena and Djukić, Aleksandra",
year = "2010",
abstract = "The lactic acid production from corn thin stillage using Lactobacillus rhamnosus ATCC 7469 in a batch system was studied. The fermentation temperature was optimized taking into account lactic acid production and glucose consumption as well as other significant process parameters (such as lactic acid yield and volumetric productivity). Also, the effect of pH control on lactic acid production by addition of 1% (w/w) CaCO3 and 10M NaOH was investigated. Based on the obtained results, an optimal fermentation temperature of 41 0C was selected. In this case, lactic acid yield of 0.70 g/g and utilized glucose of 72.55% were achieved. The addition of 10 M NaOH after 72h of fermentation at optimal temperature improved substrate (glucose) utilization for 63.70% compared to the control sample.",
publisher = "Технолошко-металуршки факултет, Београд",
journal = "Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem",
title = "Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469",
pages = "51-50",
url = "https://hdl.handle.net/21.15107/rcub_technorep_6304"
}

Nikolić, S., Vukašinović-Sekulić, M., Pejin, D., Mojović, L., Rakin, M., Pejin, J.,& Djukić, A.. (2010). Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469. in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem
Технолошко-металуршки факултет, Београд., 50-51.
https://hdl.handle.net/21.15107/rcub_technorep_6304

Nikolić S, Vukašinović-Sekulić M, Pejin D, Mojović L, Rakin M, Pejin J, Djukić A. Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469. in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem. 2010;:50-51.
https://hdl.handle.net/21.15107/rcub_technorep_6304 .

Nikolić, Svetlana, Vukašinović-Sekulić, Maja, Pejin, Dušanka, Mojović, Ljiljana, Rakin, Marica, Pejin, Jelena, Djukić, Aleksandra, "Production of lactic acid from corn thin stillage using Lactobacillus rhamnosus ATCC 7469" in Biotehnologija za održivi razvoj-Nacionalna koferencija sa međunarodnim učešćem (2010):50-51,
https://hdl.handle.net/21.15107/rcub_technorep_6304 .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1636
AB  - The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield and volumetric productivity. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v v(-1)) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. Optimal initial glucose concentrations of 150 and 176 g l(-1) for free and immobilized system were achieved, respectively. The immobilized cell system was superior to the free cell system since higher ethanol tolerance and productivity and lower substrate inhibition were detected.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Biomass & Bioenergy
T1  - Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 1456
IS  - 10
SP  - 1449
VL  - 34
DO  - 10.1016/j.biombioe.2010.04.008
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Vukašinović-Sekulić, Maja",
year = "2010",
abstract = "The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield and volumetric productivity. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v v(-1)) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. Optimal initial glucose concentrations of 150 and 176 g l(-1) for free and immobilized system were achieved, respectively. The immobilized cell system was superior to the free cell system since higher ethanol tolerance and productivity and lower substrate inhibition were detected.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Biomass & Bioenergy",
title = "Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "1456-1449",
number = "10",
volume = "34",
doi = "10.1016/j.biombioe.2010.04.008"
}

Nikolić, S., Mojović, L., Pejin, D., Rakin, M.,& Vukašinović-Sekulić, M.. (2010). Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Biomass & Bioenergy
Pergamon-Elsevier Science Ltd, Oxford., 34(10), 1449-1456.
https://doi.org/10.1016/j.biombioe.2010.04.008

Nikolić S, Mojović L, Pejin D, Rakin M, Vukašinović-Sekulić M. Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Biomass & Bioenergy. 2010;34(10):1449-1456.
doi:10.1016/j.biombioe.2010.04.008 .

Nikolić, Svetlana, Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Vukašinović-Sekulić, Maja, "Production of bioethanol from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Biomass & Bioenergy, 34, no. 10 (2010):1449-1456,
https://doi.org/10.1016/j.biombioe.2010.04.008 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Pejin, Dušanka
AU  - Pejin, Jelena
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1738
AB  - Bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal by Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The SSF process kinetics were assessed and determined. Based on the obtained results, both pretreatments improved ethanol production during the SSF process. The ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process for 11.15 % and 13.40 % (compared to the control sample), respectively. The application of microwave pretreatment resulted in higher increase in ethanol concentration compared to the ultrasound pretreatment.
PB  - Aidic Servizi Srl, Milano
T2  - Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
T1  - Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments
EP  - 1332
SP  - 1327
VL  - 21
DO  - 10.3303/CET1021222
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Vukašinović-Sekulić, Maja and Pejin, Dušanka and Pejin, Jelena",
year = "2010",
abstract = "Bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal by Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system with prior ultrasound or microwave treatment was studied. The SSF process kinetics were assessed and determined. Based on the obtained results, both pretreatments improved ethanol production during the SSF process. The ultrasound and microwave pretreatments increased the maximum ethanol concentration produced in the SSF process for 11.15 % and 13.40 % (compared to the control sample), respectively. The application of microwave pretreatment resulted in higher increase in ethanol concentration compared to the ultrasound pretreatment.",
publisher = "Aidic Servizi Srl, Milano",
journal = "Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener",
title = "Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments",
pages = "1332-1327",
volume = "21",
doi = "10.3303/CET1021222"
}

Nikolić, S., Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Pejin, D.,& Pejin, J.. (2010). Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
Aidic Servizi Srl, Milano., 21, 1327-1332.
https://doi.org/10.3303/CET1021222

Nikolić S, Mojović L, Rakin M, Vukašinović-Sekulić M, Pejin D, Pejin J. Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener. 2010;21:1327-1332.
doi:10.3303/CET1021222 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Vukašinović-Sekulić, Maja, Pejin, Dušanka, Pejin, Jelena, "Improvement of Bioethanol Production from Corn by Ultrasound and Microwave Pretreatments" in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener, 21 (2010):1327-1332,
https://doi.org/10.3303/CET1021222 . .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Nikolić, Svetlana
AU  - Pejin, Jelena
AU  - Pejin, Dušanka
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1736
AB  - The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieved after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals.
PB  - Aidic Servizi Srl, Milano
T2  - Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
T1  - Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast
EP  - 1338
SP  - 1333
VL  - 21
DO  - 10.3303/CET1021223
ER  - 

@article{
author = "Mojović, Ljiljana and Rakin, Marica and Vukašinović-Sekulić, Maja and Nikolić, Svetlana and Pejin, Jelena and Pejin, Dušanka",
year = "2010",
abstract = "The production of bioethanol by simultaneous saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied in this work. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The kinetics of the SSF process with immobilized yeast was assessed with various initial glucose concentrations. In addition, the effect of media supplementation with the yeast activators such as mineral salts, ZnSO4. 7H(2)O and MgSO4. 7H(2)O and vitamins, Ca-pantothenate, biotin and myoinositol was investigated. The system was compared with the SSF system with free yeast. The immobilized system was found superior to the free system since higher ethanol concentration and process productivity were obtained. The maximum increase in ethanol concentration was achieved in immobilized system when mineral salts were added. In this case, the ethanol concentration of 9.70% (w/w) was achieved after 38 hours of fermentation. Addition of the magnesium and zinc contributed to the achievement of a high productivity of the batch SSF of corn meal with immobilized S. cerevisiae var. ellipsoideus yeast, while still preserving a physical and chemical stability of Ca-alginate gel beads. High productivity of the system was attributed to the cell protection from inhibition by ethanol that was achieved by immobilization, and the activation of the yeast metabolism that was accomplished by the minerals.",
publisher = "Aidic Servizi Srl, Milano",
journal = "Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener",
title = "Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast",
pages = "1338-1333",
volume = "21",
doi = "10.3303/CET1021223"
}

Mojović, L., Rakin, M., Vukašinović-Sekulić, M., Nikolić, S., Pejin, J.,& Pejin, D.. (2010). Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener
Aidic Servizi Srl, Milano., 21, 1333-1338.
https://doi.org/10.3303/CET1021223

Mojović L, Rakin M, Vukašinović-Sekulić M, Nikolić S, Pejin J, Pejin D. Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast. in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener. 2010;21:1333-1338.
doi:10.3303/CET1021223 .

Mojović, Ljiljana, Rakin, Marica, Vukašinović-Sekulić, Maja, Nikolić, Svetlana, Pejin, Jelena, Pejin, Dušanka, "Production of Bioethanol by Simultaneous Saccharification and Fermentation of Corn Meal by Immobilized Yeast" in Pres 2010: 13th International Conference on Process Integration, Modelling and Optimisation for Ener, 21 (2010):1333-1338,
https://doi.org/10.3303/CET1021223 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Pejin, Jelena
PY  - 2010
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1698
AB  - An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 degrees C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of corn meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal
EP  - 222
IS  - 1
SP  - 216
VL  - 122
DO  - 10.1016/j.foodchem.2010.02.063
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Pejin, Jelena",
year = "2010",
abstract = "An ultrasound-assisted liquefaction as a pretreatment for bioethanol production by simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. Ultrasound pretreatment (at a frequency of 40 kHz) was performed at different sonication times and temperatures, before addition of liquefying enzyme. An optimal duration of the treatment of 5 min and sonication temperature of 60 degrees C were selected, taking into account glucose concentration after the liquefaction step. Under the optimum conditions an increase of glucose concentration of 6.82% over untreated control sample was achieved. Furthermore, the SSF process kinetics was assessed and determined, and the effect of ultrasound pretreatment on an increase of ethanol productivity was investigated. The obtained results indicated that the ultrasound pretreatment could increase the ethanol concentration by 11.15% (compared to the control sample) as well as other significant process parameters. In this case, the maximum ethanol concentration of 9.67% w/w (which corresponded to percentage of the theoretical ethanol yield of 88.96%) was achieved after 32 h of the SSF process. A comparison of scanning electron micrographs of the ultrasound-pretreated and untreated samples of corn meal suspensions showed that the ultrasound stimulated degradation of starch granules and release of glucose, and thereby accelerated the starch hydrolysis due to the cavitation and acoustic streaming caused by the ultrasonic action.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal",
pages = "222-216",
number = "1",
volume = "122",
doi = "10.1016/j.foodchem.2010.02.063"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Pejin, J.. (2010). Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal. in Food Chemistry
Elsevier Sci Ltd, Oxford., 122(1), 216-222.
https://doi.org/10.1016/j.foodchem.2010.02.063

Nikolić S, Mojović L, Rakin M, Pejin D, Pejin J. Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal. in Food Chemistry. 2010;122(1):216-222.
doi:10.1016/j.foodchem.2010.02.063 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Pejin, Jelena, "Ultrasound-assisted production of bioethanol by simultaneous saccharification and fermentation of corn meal" in Food Chemistry, 122, no. 1 (2010):216-222,
https://doi.org/10.1016/j.foodchem.2010.02.063 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1500
AB  - The simultaneous enzymatic saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 center dot 7H(2)O and MgSO4 center dot 7H(2)O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/l.h were obtained.
PB  - Elsevier Sci Ltd, Oxford
T2  - Fuel
T1  - Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 1607
IS  - 9
SP  - 1602
VL  - 88
DO  - 10.1016/j.fuel.2008.12.019
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka",
year = "2009",
abstract = "The simultaneous enzymatic saccharification and fermentation (SSF) of corn meal using immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The yeast cells were immobilized in Ca-alginate by electrostatic droplet generation method. The process kinetics was assessed and determined and the effect of addition of various yeast activators (mineral salts: ZnSO4 center dot 7H(2)O and MgSO4 center dot 7H(2)O, and vitamins: Ca-pantothenate, biotin and myo-inositol) separately or mixed, was investigated. Taking into account high values of process parameters (such as ethanol concentration, ethanol yield, percentage of the theoretical ethanol yield, volumetric productivity and utilized glucose) and significant energy savings the SSF process was found to be superior compared to the SHF process. Further improvement in ethanol production was accomplished with the addition of mineral salts as yeast activators which contributed to the highest increase in ethanol production. In this case, the ethanol concentration of 10.23% (w/w), percentage of the theoretical ethanol yield of 98.08%, the ethanol yield of 0.55 g/g and the volumetric productivity of 2.13 g/l.h were obtained.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fuel",
title = "Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "1607-1602",
number = "9",
volume = "88",
doi = "10.1016/j.fuel.2008.12.019"
}

Nikolić, S., Mojović, L., Rakin, M.,& Pejin, D.. (2009). Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Fuel
Elsevier Sci Ltd, Oxford., 88(9), 1602-1607.
https://doi.org/10.1016/j.fuel.2008.12.019

Nikolić S, Mojović L, Rakin M, Pejin D. Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Fuel. 2009;88(9):1602-1607.
doi:10.1016/j.fuel.2008.12.019 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, "Bioethanol production from corn meal by simultaneous enzymatic saccharification and fermentation with immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Fuel, 88, no. 9 (2009):1602-1607,
https://doi.org/10.1016/j.fuel.2008.12.019 . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Nedović, Viktor
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1485
AB  - BACKGROUND: Bioethanol produced from renewable biomass, such as corn meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca-alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L-1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca-alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca-alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed.
PB  - Wiley, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus
EP  - 503
IS  - 4
SP  - 497
VL  - 84
DO  - 10.1002/jctb.2068
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Nedović, Viktor",
year = "2009",
abstract = "BACKGROUND: Bioethanol produced from renewable biomass, such as corn meal, is a biofuel that is both renewable and environmentally friendly. Significant scientific and technological investments will be needed to achieve substitution of conventional fossil fuels with alternative fuels. The ethanol fermentation of enzymatically obtained corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. The initial glucose and inoculum concentration and the time required for the efficient ethanol production were optimized taking into account parameters such as ethanol concentration, ethanol yield, percentage of the theoretical yield of ethanol and volumetric productivity in both immobilized and free cell systems. RESULTS: The yeast cells were immobilized in Ca-alginate by an electrostatic droplet generation method. An optimal initial inoculum concentration of 2% (v/v) and optimal fermentation time of 38 h for both immobilized and free yeasts were determined. An optimal initial glucose concentration of 150 g L-1 for free system was achieved. At the initial glucose concentration of 176 g L no substrate or product inhibition were achieved with immobilized yeast. CONCLUSION: By immobilization of the yeast into Ca-alginate using the method of electrostatic droplet generation a superior system was realized, which exhibited lower substrate inhibition and higher tolerance to ethanol. The cells of S. cerevisiae var. ellipsoideus yeast entrapped in Ca-alginate showed good physical and chemical stability, and no substrate and product diffusion restrictions were noticed.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus",
pages = "503-497",
number = "4",
volume = "84",
doi = "10.1002/jctb.2068"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Nedović, V.. (2009). Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 84(4), 497-503.
https://doi.org/10.1002/jctb.2068

Nikolić S, Mojović L, Rakin M, Pejin D, Nedović V. Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus. in Journal of Chemical Technology and Biotechnology. 2009;84(4):497-503.
doi:10.1002/jctb.2068 .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Nedović, Viktor, "Effect of different fermentation parameters on bioethanol production from corn meal hydrolyzates by free and immobilized cells of Saccharomyces cerevisiae var. ellipsoideus" in Journal of Chemical Technology and Biotechnology, 84, no. 4 (2009):497-503,
https://doi.org/10.1002/jctb.2068 . .

TY  - JOUR
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Grujić, Olgica
AU  - Markov, Siniša
AU  - Pejin, Jelena
AU  - Rakin, Marica
AU  - Vukašinović-Sekulić, Maja
AU  - Nikolić, Svetlana
AU  - Savić, Dragiša
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1446
AB  - Bioethanol produced from renewable biomass, such as sugar, starch, or lignocellulosic materials, is one of the alternative energy resources, which is both renewable and environmentally friendly. Although, the priority in global future ethanol production is put on lignocellulosic processing, which is considered as one of the most promising second-generation biofuel technologies, the utilization of lignocellulosic material for fuel ethanol is still under improvement. Sugar-based (molasses, sugar cane, sugar beet) and starch-based (corn, wheat, triticale, potato, rice, etc.) feedstock are still currently predominant at the industrial level and they are, so far, economically favorable compared to lingocelluloses. Currently, approx. 80 % of total world ethanol production is obtained from the fermentation of simple sugars by yeast. In Serbia, one of the most suitable and available agricultural raw material for the industrial ethanol production are cereals such as corn, wheat and triticale. In addition, surpluses of this feedstock are being produced in our country constantly. In this paper, a brief review of the state of the art in bioethanol production and biomass availability is given, pointing out the progress possibilities on starch-based production. The progress possibilities are discussed in the domain of feedstock choice and pretreatment, optimization of fermentation, process integration and utilization of the process byproducts.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Progress in the production of bioethanol on starch-based feedstocks
EP  - 226
IS  - 4
SP  - 211
VL  - 15
DO  - 10.2298/CICEQ0904211M
ER  - 

@article{
author = "Mojović, Ljiljana and Pejin, Dušanka and Grujić, Olgica and Markov, Siniša and Pejin, Jelena and Rakin, Marica and Vukašinović-Sekulić, Maja and Nikolić, Svetlana and Savić, Dragiša",
year = "2009",
abstract = "Bioethanol produced from renewable biomass, such as sugar, starch, or lignocellulosic materials, is one of the alternative energy resources, which is both renewable and environmentally friendly. Although, the priority in global future ethanol production is put on lignocellulosic processing, which is considered as one of the most promising second-generation biofuel technologies, the utilization of lignocellulosic material for fuel ethanol is still under improvement. Sugar-based (molasses, sugar cane, sugar beet) and starch-based (corn, wheat, triticale, potato, rice, etc.) feedstock are still currently predominant at the industrial level and they are, so far, economically favorable compared to lingocelluloses. Currently, approx. 80 % of total world ethanol production is obtained from the fermentation of simple sugars by yeast. In Serbia, one of the most suitable and available agricultural raw material for the industrial ethanol production are cereals such as corn, wheat and triticale. In addition, surpluses of this feedstock are being produced in our country constantly. In this paper, a brief review of the state of the art in bioethanol production and biomass availability is given, pointing out the progress possibilities on starch-based production. The progress possibilities are discussed in the domain of feedstock choice and pretreatment, optimization of fermentation, process integration and utilization of the process byproducts.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Progress in the production of bioethanol on starch-based feedstocks",
pages = "226-211",
number = "4",
volume = "15",
doi = "10.2298/CICEQ0904211M"
}

Mojović, L., Pejin, D., Grujić, O., Markov, S., Pejin, J., Rakin, M., Vukašinović-Sekulić, M., Nikolić, S.,& Savić, D.. (2009). Progress in the production of bioethanol on starch-based feedstocks. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 15(4), 211-226.
https://doi.org/10.2298/CICEQ0904211M

Mojović L, Pejin D, Grujić O, Markov S, Pejin J, Rakin M, Vukašinović-Sekulić M, Nikolić S, Savić D. Progress in the production of bioethanol on starch-based feedstocks. in Chemical Industry & Chemical Engineering Quarterly. 2009;15(4):211-226.
doi:10.2298/CICEQ0904211M .

Mojović, Ljiljana, Pejin, Dušanka, Grujić, Olgica, Markov, Siniša, Pejin, Jelena, Rakin, Marica, Vukašinović-Sekulić, Maja, Nikolić, Svetlana, Savić, Dragiša, "Progress in the production of bioethanol on starch-based feedstocks" in Chemical Industry & Chemical Engineering Quarterly, 15, no. 4 (2009):211-226,
https://doi.org/10.2298/CICEQ0904211M . .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Pejin, Dušanka
AU  - Rakin, Marica
AU  - Vucurović, Vesna
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1363
AB  - The possibilities of improving ethanol fermentation of enzymatically obtained corn semolina hydrolyzates with alginate-immobilized yeast Saccharomyces cerevisiae var. ellipsoideus by medium supplementation with mineral salts as sources of magnesium, zinc, calcium and copper ions, and vitamins (pantothenate, thiamine, pyridoxine, biotin and inositol), separately or as combined mixtures, have been investigated. Among all tested minerals, alone or combined, the most efficient in improving ethanol productivity during fermentation of corn semolina hydrolyzates was a mixture of magnesium and zinc salts: MgSO4 (2 g/L) and ZnSO4 (0.3 g/L). Positive effects were also obtained with the addition of copper ions (CuCl2, 1 mg/L) or calcium ions (CaCl2, 40 mg/L). Among vitamins, the most effective was Ca-pantothenate (1 g/L), which caused an increase in the fermentation efficiency for approx. 8 %, compared to the control sample. Based on these results, an effective mixture of vitamins and minerals consisting Of MgSO4 (2 g/L), ZnSO4 (0.3 g/L), CuCl2 (1 mg/L), Ca-pantothenate (1 g/L) and inositol (1 g/L) was arranged for the supplementation of the medium based on corn semolina hydrolyzates. The supplementation with this Mixture provided an increase of the fermentation efficiency for 20 % compared to the control sample, without supplementation.
PB  - University of Zagreb
T2  - Food Technology and Biotechnology
T1  - Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation
EP  - 89
IS  - 1
SP  - 83
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1363
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Pejin, Dušanka and Rakin, Marica and Vucurović, Vesna",
year = "2009",
abstract = "The possibilities of improving ethanol fermentation of enzymatically obtained corn semolina hydrolyzates with alginate-immobilized yeast Saccharomyces cerevisiae var. ellipsoideus by medium supplementation with mineral salts as sources of magnesium, zinc, calcium and copper ions, and vitamins (pantothenate, thiamine, pyridoxine, biotin and inositol), separately or as combined mixtures, have been investigated. Among all tested minerals, alone or combined, the most efficient in improving ethanol productivity during fermentation of corn semolina hydrolyzates was a mixture of magnesium and zinc salts: MgSO4 (2 g/L) and ZnSO4 (0.3 g/L). Positive effects were also obtained with the addition of copper ions (CuCl2, 1 mg/L) or calcium ions (CaCl2, 40 mg/L). Among vitamins, the most effective was Ca-pantothenate (1 g/L), which caused an increase in the fermentation efficiency for approx. 8 %, compared to the control sample. Based on these results, an effective mixture of vitamins and minerals consisting Of MgSO4 (2 g/L), ZnSO4 (0.3 g/L), CuCl2 (1 mg/L), Ca-pantothenate (1 g/L) and inositol (1 g/L) was arranged for the supplementation of the medium based on corn semolina hydrolyzates. The supplementation with this Mixture provided an increase of the fermentation efficiency for 20 % compared to the control sample, without supplementation.",
publisher = "University of Zagreb",
journal = "Food Technology and Biotechnology",
title = "Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation",
pages = "89-83",
number = "1",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1363"
}

Nikolić, S., Mojović, L., Pejin, D., Rakin, M.,& Vucurović, V.. (2009). Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation. in Food Technology and Biotechnology
University of Zagreb., 47(1), 83-89.
https://hdl.handle.net/21.15107/rcub_technorep_1363

Nikolić S, Mojović L, Pejin D, Rakin M, Vucurović V. Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation. in Food Technology and Biotechnology. 2009;47(1):83-89.
https://hdl.handle.net/21.15107/rcub_technorep_1363 .

Nikolić, Svetlana, Mojović, Ljiljana, Pejin, Dušanka, Rakin, Marica, Vucurović, Vesna, "Improvement of Ethanol Fermentation of Corn Semolina Hydrolyzates with Immobilized Yeast by Medium Supplementation" in Food Technology and Biotechnology, 47, no. 1 (2009):83-89,
https://hdl.handle.net/21.15107/rcub_technorep_1363 .

TY  - JOUR
AU  - Rakin, Marica
AU  - Mojović, Ljiljana
AU  - Nikolić, Svetlana
AU  - Vukašinović-Sekulić, Maja
AU  - Nedović, Viktor
PY  - 2009
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1362
AB  - Bioethanol can be produced by fermentation of sugars from various waste agricultural materials. Whichever system for bioethanol production is chosen, the attention must be paid to the overall economics and energy consumption. The aim of the present study was to investigate the immobilization of Sacharomyces cerevisiae var. ellipsoideus yeast cells for bioethanol production from corn meal hydrolyzates. For this purpose the biocompatible polymers such as polyvinil alcohol (PVA) and Ca-alginate were assessed. The parameters of ethanol fermentation, such as inoculum concentration in different carriers and the choice of a convenient carrier for the efficient ethanol production were studied. The maximum ethanol concentration of 10.05% (w/w) was obtained in the fermentation of corn meal hydrolyzates by 5% (v/v) of inoculum concentration of the yeast immobilized in Ca-alginate using a method of electrostatic droplet generation. The repeated batch fermentation with the yeast immobilized in Ca-alginate indicated that alginate gels degraded after the second fermentation cycle. PVA carrier exhibited better mechanical properties and stability; however lower ethanol concentrations were achieved during the fermentation.
T2  - African Journal of Biotechnology
T1  - Bioethanol production by immobilized Sacharomyces cerevisiae var. ellipsoideus cells
EP  - 471
IS  - 3
SP  - 464
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_technorep_1362
ER  - 

@article{
author = "Rakin, Marica and Mojović, Ljiljana and Nikolić, Svetlana and Vukašinović-Sekulić, Maja and Nedović, Viktor",
year = "2009",
abstract = "Bioethanol can be produced by fermentation of sugars from various waste agricultural materials. Whichever system for bioethanol production is chosen, the attention must be paid to the overall economics and energy consumption. The aim of the present study was to investigate the immobilization of Sacharomyces cerevisiae var. ellipsoideus yeast cells for bioethanol production from corn meal hydrolyzates. For this purpose the biocompatible polymers such as polyvinil alcohol (PVA) and Ca-alginate were assessed. The parameters of ethanol fermentation, such as inoculum concentration in different carriers and the choice of a convenient carrier for the efficient ethanol production were studied. The maximum ethanol concentration of 10.05% (w/w) was obtained in the fermentation of corn meal hydrolyzates by 5% (v/v) of inoculum concentration of the yeast immobilized in Ca-alginate using a method of electrostatic droplet generation. The repeated batch fermentation with the yeast immobilized in Ca-alginate indicated that alginate gels degraded after the second fermentation cycle. PVA carrier exhibited better mechanical properties and stability; however lower ethanol concentrations were achieved during the fermentation.",
journal = "African Journal of Biotechnology",
title = "Bioethanol production by immobilized Sacharomyces cerevisiae var. ellipsoideus cells",
pages = "471-464",
number = "3",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_technorep_1362"
}

Rakin, M., Mojović, L., Nikolić, S., Vukašinović-Sekulić, M.,& Nedović, V.. (2009). Bioethanol production by immobilized Sacharomyces cerevisiae var. ellipsoideus cells. in African Journal of Biotechnology, 8(3), 464-471.
https://hdl.handle.net/21.15107/rcub_technorep_1362

Rakin M, Mojović L, Nikolić S, Vukašinović-Sekulić M, Nedović V. Bioethanol production by immobilized Sacharomyces cerevisiae var. ellipsoideus cells. in African Journal of Biotechnology. 2009;8(3):464-471.
https://hdl.handle.net/21.15107/rcub_technorep_1362 .

Rakin, Marica, Mojović, Ljiljana, Nikolić, Svetlana, Vukašinović-Sekulić, Maja, Nedović, Viktor, "Bioethanol production by immobilized Sacharomyces cerevisiae var. ellipsoideus cells" in African Journal of Biotechnology, 8, no. 3 (2009):464-471,
https://hdl.handle.net/21.15107/rcub_technorep_1362 .

TY  - JOUR
AU  - Nikolić, Svetlana
AU  - Mojović, Ljiljana
AU  - Rakin, Marica
AU  - Pejin, Dušanka
AU  - Savić, Dragiša
PY  - 2008
UR  - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1340
AB  - A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.
PB  - Association of the Chemical Engineers of Serbia
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal
EP  - 234
IS  - 4
SP  - 231
VL  - 14
DO  - 10.2298/CICEQ0804231N
ER  - 

@article{
author = "Nikolić, Svetlana and Mojović, Ljiljana and Rakin, Marica and Pejin, Dušanka and Savić, Dragiša",
year = "2008",
abstract = "A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation (SSF) of corn meal using Saccharomyces cerevisiae var. ellipsoideus yeast in a batch system was studied. An optimal power of microwaves of 80 W and the 5-min duration of the microwave treatment were selected by following the concentration of glucose released from the corn meal suspensions at hidromodul of 1:3 (corn meal to water ratio) in the liquefaction step. The results indicated that the microwave pretreatment could increase the maximum ethanol concentration produced in the SSF process for 13.4 %. Consequently, a significant increase of the ethanol productivity on substrate (YP/S), as well as the volumetric ethanol productivity (P) in this process, could be achieved.",
publisher = "Association of the Chemical Engineers of Serbia",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal",
pages = "234-231",
number = "4",
volume = "14",
doi = "10.2298/CICEQ0804231N"
}

Nikolić, S., Mojović, L., Rakin, M., Pejin, D.,& Savić, D.. (2008). A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal. in Chemical Industry & Chemical Engineering Quarterly
Association of the Chemical Engineers of Serbia., 14(4), 231-234.
https://doi.org/10.2298/CICEQ0804231N

Nikolić S, Mojović L, Rakin M, Pejin D, Savić D. A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal. in Chemical Industry & Chemical Engineering Quarterly. 2008;14(4):231-234.
doi:10.2298/CICEQ0804231N .

Nikolić, Svetlana, Mojović, Ljiljana, Rakin, Marica, Pejin, Dušanka, Savić, Dragiša, "A microwave-assisted liquefaction as a pretreatment for the bioethanol production by the simultaneous saccharification and fermentation of corn meal" in Chemical Industry & Chemical Engineering Quarterly, 14, no. 4 (2008):231-234,
https://doi.org/10.2298/CICEQ0804231N . .