Lipase-catalyzed biodiesel synthesis with different acyl acceptors
Primena novih acil akceptora u procesu enzimski katalizovane sinteze biodizela
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Biodiesel is an alternative fuel for diesel engine that is environmentally acceptable. Conventionally, biodiesel is produced by transesterification of triglycerides and short alcohols in the presence of an acid or an alkaline catalyst. There are several problems associated with this kind of production that can be resolved by using lipase as the biocatalyst. The aim of the present work was to investigate novel acyl acceptors for biodiesel production. 2-Propanol and n-butanol have a less negative effect on lipase stability, and they also improve low temperature properties of the fuel. However, excess alcohol leads to inactivation of the enzyme, and glycerol, a major byproduct, can block the immobilized enzyme, resulting in low enzymatic activity. This problem was solved by using methyl acetate as acyl acceptor. Triacetylglycerol is produced instead of glycerol, and it has no negative effect on the activity of the lipase.
U ovom radu ispitana je mogućnost primene različitih acil akceptora kao polaznih reaktanata umesto metanola u sintezi biodizela u cilju smanjenja inhibicije enzima, povećanja njegove stabilnosti i mogućnosti značajnog pojednostavljivanja izvođenja procesa. Prvo je ispitana mogućnost korišćenja različitih alkohola: 2-propanol i n-butanol koji imaju manji uticaj na denaturaciju enzima. Osim toga, primenom ovih alkohola dobija se gorivo boljeg kvaliteta, pošto se sa povećanjem broja ugljenikovih atoma povećava oktanski broj, temperatura paljenja, kao i sadržaj toplote. Drugi deo istraživanja bio je orijentisan ka ispitivanju mogućnosti korišćenja metilacetata kao pogodnog nukleofila. Za razliku od alkohola, primenom ovog acil akceptora omogućen je jednostepeni postupak sinteze biodizela, što značajno smanjuje vreme trajanja reakcije, kao i kompleksnost iste. Takođe, primenom metilacetata povećava se stabilnost biokatalizatora, što omogućava višekratnu upotrebu. Naime, u reakciji sa metila...cetatom kao nusprodukt se ne stvara glicerol već triacetilglicerol, koji nema negativan efekat na stabilnost lipaze.
Keywords:
biodiesel / lipase / methanol / 2-propanol / n-butanol / methyl acetateSource:
Acta periodica technologica, 2008, 39, 161-169Publisher:
- Faculty of Technology, Novi Sad
Funding / projects:
- Razvoj biotehnoloških postupaka za proizvodnju aditiva i novih formulacija za prehrambenu industriju (RS-MESTD-MPN2006-2010-20064)
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Ognjanović, Nevena AU - Šaponjić, Svetlana V. AU - Bezbradica, Dejan AU - Knežević, Zorica PY - 2008 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1279 AB - Biodiesel is an alternative fuel for diesel engine that is environmentally acceptable. Conventionally, biodiesel is produced by transesterification of triglycerides and short alcohols in the presence of an acid or an alkaline catalyst. There are several problems associated with this kind of production that can be resolved by using lipase as the biocatalyst. The aim of the present work was to investigate novel acyl acceptors for biodiesel production. 2-Propanol and n-butanol have a less negative effect on lipase stability, and they also improve low temperature properties of the fuel. However, excess alcohol leads to inactivation of the enzyme, and glycerol, a major byproduct, can block the immobilized enzyme, resulting in low enzymatic activity. This problem was solved by using methyl acetate as acyl acceptor. Triacetylglycerol is produced instead of glycerol, and it has no negative effect on the activity of the lipase. AB - U ovom radu ispitana je mogućnost primene različitih acil akceptora kao polaznih reaktanata umesto metanola u sintezi biodizela u cilju smanjenja inhibicije enzima, povećanja njegove stabilnosti i mogućnosti značajnog pojednostavljivanja izvođenja procesa. Prvo je ispitana mogućnost korišćenja različitih alkohola: 2-propanol i n-butanol koji imaju manji uticaj na denaturaciju enzima. Osim toga, primenom ovih alkohola dobija se gorivo boljeg kvaliteta, pošto se sa povećanjem broja ugljenikovih atoma povećava oktanski broj, temperatura paljenja, kao i sadržaj toplote. Drugi deo istraživanja bio je orijentisan ka ispitivanju mogućnosti korišćenja metilacetata kao pogodnog nukleofila. Za razliku od alkohola, primenom ovog acil akceptora omogućen je jednostepeni postupak sinteze biodizela, što značajno smanjuje vreme trajanja reakcije, kao i kompleksnost iste. Takođe, primenom metilacetata povećava se stabilnost biokatalizatora, što omogućava višekratnu upotrebu. Naime, u reakciji sa metilacetatom kao nusprodukt se ne stvara glicerol već triacetilglicerol, koji nema negativan efekat na stabilnost lipaze. PB - Faculty of Technology, Novi Sad T2 - Acta periodica technologica T1 - Lipase-catalyzed biodiesel synthesis with different acyl acceptors T1 - Primena novih acil akceptora u procesu enzimski katalizovane sinteze biodizela EP - 169 IS - 39 SP - 161 DO - 10.2298/APT0839161O ER -
@article{ author = "Ognjanović, Nevena and Šaponjić, Svetlana V. and Bezbradica, Dejan and Knežević, Zorica", year = "2008", abstract = "Biodiesel is an alternative fuel for diesel engine that is environmentally acceptable. Conventionally, biodiesel is produced by transesterification of triglycerides and short alcohols in the presence of an acid or an alkaline catalyst. There are several problems associated with this kind of production that can be resolved by using lipase as the biocatalyst. The aim of the present work was to investigate novel acyl acceptors for biodiesel production. 2-Propanol and n-butanol have a less negative effect on lipase stability, and they also improve low temperature properties of the fuel. However, excess alcohol leads to inactivation of the enzyme, and glycerol, a major byproduct, can block the immobilized enzyme, resulting in low enzymatic activity. This problem was solved by using methyl acetate as acyl acceptor. Triacetylglycerol is produced instead of glycerol, and it has no negative effect on the activity of the lipase., U ovom radu ispitana je mogućnost primene različitih acil akceptora kao polaznih reaktanata umesto metanola u sintezi biodizela u cilju smanjenja inhibicije enzima, povećanja njegove stabilnosti i mogućnosti značajnog pojednostavljivanja izvođenja procesa. Prvo je ispitana mogućnost korišćenja različitih alkohola: 2-propanol i n-butanol koji imaju manji uticaj na denaturaciju enzima. Osim toga, primenom ovih alkohola dobija se gorivo boljeg kvaliteta, pošto se sa povećanjem broja ugljenikovih atoma povećava oktanski broj, temperatura paljenja, kao i sadržaj toplote. Drugi deo istraživanja bio je orijentisan ka ispitivanju mogućnosti korišćenja metilacetata kao pogodnog nukleofila. Za razliku od alkohola, primenom ovog acil akceptora omogućen je jednostepeni postupak sinteze biodizela, što značajno smanjuje vreme trajanja reakcije, kao i kompleksnost iste. Takođe, primenom metilacetata povećava se stabilnost biokatalizatora, što omogućava višekratnu upotrebu. Naime, u reakciji sa metilacetatom kao nusprodukt se ne stvara glicerol već triacetilglicerol, koji nema negativan efekat na stabilnost lipaze.", publisher = "Faculty of Technology, Novi Sad", journal = "Acta periodica technologica", title = "Lipase-catalyzed biodiesel synthesis with different acyl acceptors, Primena novih acil akceptora u procesu enzimski katalizovane sinteze biodizela", pages = "169-161", number = "39", doi = "10.2298/APT0839161O" }
Ognjanović, N., Šaponjić, S. V., Bezbradica, D.,& Knežević, Z.. (2008). Lipase-catalyzed biodiesel synthesis with different acyl acceptors. in Acta periodica technologica Faculty of Technology, Novi Sad.(39), 161-169. https://doi.org/10.2298/APT0839161O
Ognjanović N, Šaponjić SV, Bezbradica D, Knežević Z. Lipase-catalyzed biodiesel synthesis with different acyl acceptors. in Acta periodica technologica. 2008;(39):161-169. doi:10.2298/APT0839161O .
Ognjanović, Nevena, Šaponjić, Svetlana V., Bezbradica, Dejan, Knežević, Zorica, "Lipase-catalyzed biodiesel synthesis with different acyl acceptors" in Acta periodica technologica, no. 39 (2008):161-169, https://doi.org/10.2298/APT0839161O . .