A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone
Autori
Prlainović, NevenaBezbradica, Dejan
Knežević-Jugović, Zorica
Kozlowska, Roksana T.
Mijin, Dušan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 degrees C with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.
Ključne reči:
Candida rugosa lipase / ping-pong kinetics / cyanoacetamide inhibition / acetylacetone / pyridoneIzvor:
Journal of the Brazilian Chemical Society, 2010, 21, 12, 2285-2293Izdavač:
- Sociedade Brasileira de Química
Finansiranje / projekti:
- Proučavanje sinteze, strukture i aktivnosti organskih jedinjenja prirodnog i sintetskog porekla (RS-MESTD-MPN2006-2010-142063)
- Razvoj biotehnoloških postupaka za proizvodnju aditiva i novih formulacija za prehrambenu industriju (RS-MESTD-MPN2006-2010-20064)
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Prlainović, Nevena AU - Bezbradica, Dejan AU - Knežević-Jugović, Zorica AU - Kozlowska, Roksana T. AU - Mijin, Dušan PY - 2010 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1563 AB - The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 degrees C with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed. PB - Sociedade Brasileira de Química T2 - Journal of the Brazilian Chemical Society T1 - A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone EP - 2293 IS - 12 SP - 2285 VL - 21 UR - https://hdl.handle.net/21.15107/rcub_technorep_1563 ER -
@article{ author = "Prlainović, Nevena and Bezbradica, Dejan and Knežević-Jugović, Zorica and Kozlowska, Roksana T. and Mijin, Dušan", year = "2010", abstract = "The synthesis of 4,6-dimethyl-3-cyano-2-pyridone was studied in a reaction catalyzed with Candida rugosa lipase. A response surface methodology (RSM) was applied for the optimization of the experimental conditions. The study showed that temperature and molar ratio cyanoacetamide/acetylacetone have a great influence on the yield of the reaction; the maximum yield was achieved at 44 degrees C with a molar ratio cyanoacetamide/acetylacetone of 36. The kinetics of the enzymatic synthesis of 4,6-dimethyl-3-cyano-2-pyridone was investigated, and it was determined that a ping-pong bi-ter mechanism with cyanoacetamide inhibition fits the obtained results. The mechanism of lipase-catalyzed reaction was proposed.", publisher = "Sociedade Brasileira de Química", journal = "Journal of the Brazilian Chemical Society", title = "A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone", pages = "2293-2285", number = "12", volume = "21", url = "https://hdl.handle.net/21.15107/rcub_technorep_1563" }
Prlainović, N., Bezbradica, D., Knežević-Jugović, Z., Kozlowska, R. T.,& Mijin, D.. (2010). A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone. in Journal of the Brazilian Chemical Society Sociedade Brasileira de Química., 21(12), 2285-2293. https://hdl.handle.net/21.15107/rcub_technorep_1563
Prlainović N, Bezbradica D, Knežević-Jugović Z, Kozlowska RT, Mijin D. A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone. in Journal of the Brazilian Chemical Society. 2010;21(12):2285-2293. https://hdl.handle.net/21.15107/rcub_technorep_1563 .
Prlainović, Nevena, Bezbradica, Dejan, Knežević-Jugović, Zorica, Kozlowska, Roksana T., Mijin, Dušan, "A Kinetic Study of Candida rugosa Lipase-Catalyzed Synthesis of 4,6-Dimethyl-3-cyano-2-pyridone" in Journal of the Brazilian Chemical Society, 21, no. 12 (2010):2285-2293, https://hdl.handle.net/21.15107/rcub_technorep_1563 .