Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants
Imobilizacija lipaze iz Candida rugosa u kopolimerne hidrogelove poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu površinski aktivnih materija
Аутори
Milašinović, NikolaMilosavljević, Nedeljko
Filipović, Jovanka M.
Knežević-Jugović, Zorica
Kalagasidis Krušić, Melina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
To overcome the problems of free enzyme application as catalysts in chemical reactions, i.e., high costs of isolation and purification processes, high sensitivity to process conditions, insufficient enzyme stability etc., different immobilization techniques need to be used. Immobilization to/within solid support improves enzyme stability decreasing its denaturation. This paper deals with hydrogels of N-isopropylacrylamide and itaconic acid with incorporated nonionic surfactants (Triton X-100, Brij 30 and Tween 80) synthesized in distilled water at room temperature by free radical polymerization. These hydrogels were used as supports for immobilization of enzyme, lipase from Candida rugosa by post-entrapment method. The aim was to investigate the effect of the non-ionic surfactants on the lipase binding capacity, as well as on its hydrolytic activity. In order to characterize the obtained hydrogels, FT-IR analysis has been performed. Further, the swelling behaviour of these samples in b...uffer solution of pH 6.80 has been investigated. The dynamic-mechanical properties of hydrogels have been studied, too. The immobilized lipase showed somewhat reduced hydrolytic activity, compared to the activity of free lipase as well as in comparison to the lipase immobilized to the reference sample (sample synthesized under the same polymerization conditions, but in the absence of surfactants). It was concluded that the addition of surfactants increased the hydrogel mesh size. The surfactant addition did not affect the dynamic-mechanical properties of the investigated hydrogels. The largest percentage of specific activity and yield of activity were presented by the reference sample, too. It is obvious that the absence of surfactants charged groups has no influence on the lipase binding capacity, and the obtained activity yields are to be expected.
U radu je ispitana imobilizacija lipaze iz Candida rugosa u hidrogel poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu nejonskih površinski aktivnih materija. Pri sintezi je varirana vrsta i sadržaj površinski aktivnih materija. Ispitana su mehanička svojstva sintetisanih hidrogelova i urađena je FT-IR analiza. Imobilizacija lipaze je izvedena bubrenjem prethodno sintetisanih hidrogelova u rastvoru lipaze, a zatim je ispitan uticaj dodatka površinski aktivnih materija pri sintezi nosača na kapacitet uklapanja lipaze u hidrogel i na aktivnost tako uklopljene lipaze. Imobilisana lipaza iz Candida rugosa je pokazala umanjenu hidrolitičku aktivnost, kako u odnosu na aktivnost slobodne lipaze, što je i očekivano, tako i u odnosu na aktivnost lipaze imobilisane u referentni hidrogel, sintetisan bez prisustva površinski aktivnih materija.
Кључне речи:
non-ionic surfactants / hydrogels / N-isopropylacrylamide / itaconic acid / lipase activity / nejonski surfaktanti / hidrogelovi / N-izopropilakrilamid / itakonska kiselina / aktivnost lipazeИзвор:
Hemijska industrija, 2011, 65, 6, 667-673Издавач:
- Association of Chemical Engineers of Serbia
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Синтеза и карактеризација нових функционалних полимера и полимерних нанокомпозита (RS-MESTD-Basic Research (BR or ON)-172062)
DOI: 10.2298/HEMIND110915063M
ISSN: 0367-598X
WoS: 000302108800005
Scopus: 2-s2.0-84855246653
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Milašinović, Nikola AU - Milosavljević, Nedeljko AU - Filipović, Jovanka M. AU - Knežević-Jugović, Zorica AU - Kalagasidis Krušić, Melina PY - 2011 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1806 AB - To overcome the problems of free enzyme application as catalysts in chemical reactions, i.e., high costs of isolation and purification processes, high sensitivity to process conditions, insufficient enzyme stability etc., different immobilization techniques need to be used. Immobilization to/within solid support improves enzyme stability decreasing its denaturation. This paper deals with hydrogels of N-isopropylacrylamide and itaconic acid with incorporated nonionic surfactants (Triton X-100, Brij 30 and Tween 80) synthesized in distilled water at room temperature by free radical polymerization. These hydrogels were used as supports for immobilization of enzyme, lipase from Candida rugosa by post-entrapment method. The aim was to investigate the effect of the non-ionic surfactants on the lipase binding capacity, as well as on its hydrolytic activity. In order to characterize the obtained hydrogels, FT-IR analysis has been performed. Further, the swelling behaviour of these samples in buffer solution of pH 6.80 has been investigated. The dynamic-mechanical properties of hydrogels have been studied, too. The immobilized lipase showed somewhat reduced hydrolytic activity, compared to the activity of free lipase as well as in comparison to the lipase immobilized to the reference sample (sample synthesized under the same polymerization conditions, but in the absence of surfactants). It was concluded that the addition of surfactants increased the hydrogel mesh size. The surfactant addition did not affect the dynamic-mechanical properties of the investigated hydrogels. The largest percentage of specific activity and yield of activity were presented by the reference sample, too. It is obvious that the absence of surfactants charged groups has no influence on the lipase binding capacity, and the obtained activity yields are to be expected. AB - U radu je ispitana imobilizacija lipaze iz Candida rugosa u hidrogel poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu nejonskih površinski aktivnih materija. Pri sintezi je varirana vrsta i sadržaj površinski aktivnih materija. Ispitana su mehanička svojstva sintetisanih hidrogelova i urađena je FT-IR analiza. Imobilizacija lipaze je izvedena bubrenjem prethodno sintetisanih hidrogelova u rastvoru lipaze, a zatim je ispitan uticaj dodatka površinski aktivnih materija pri sintezi nosača na kapacitet uklapanja lipaze u hidrogel i na aktivnost tako uklopljene lipaze. Imobilisana lipaza iz Candida rugosa je pokazala umanjenu hidrolitičku aktivnost, kako u odnosu na aktivnost slobodne lipaze, što je i očekivano, tako i u odnosu na aktivnost lipaze imobilisane u referentni hidrogel, sintetisan bez prisustva površinski aktivnih materija. PB - Association of Chemical Engineers of Serbia T2 - Hemijska industrija T1 - Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants T1 - Imobilizacija lipaze iz Candida rugosa u kopolimerne hidrogelove poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu površinski aktivnih materija EP - 673 IS - 6 SP - 667 VL - 65 DO - 10.2298/HEMIND110915063M ER -
@article{ author = "Milašinović, Nikola and Milosavljević, Nedeljko and Filipović, Jovanka M. and Knežević-Jugović, Zorica and Kalagasidis Krušić, Melina", year = "2011", abstract = "To overcome the problems of free enzyme application as catalysts in chemical reactions, i.e., high costs of isolation and purification processes, high sensitivity to process conditions, insufficient enzyme stability etc., different immobilization techniques need to be used. Immobilization to/within solid support improves enzyme stability decreasing its denaturation. This paper deals with hydrogels of N-isopropylacrylamide and itaconic acid with incorporated nonionic surfactants (Triton X-100, Brij 30 and Tween 80) synthesized in distilled water at room temperature by free radical polymerization. These hydrogels were used as supports for immobilization of enzyme, lipase from Candida rugosa by post-entrapment method. The aim was to investigate the effect of the non-ionic surfactants on the lipase binding capacity, as well as on its hydrolytic activity. In order to characterize the obtained hydrogels, FT-IR analysis has been performed. Further, the swelling behaviour of these samples in buffer solution of pH 6.80 has been investigated. The dynamic-mechanical properties of hydrogels have been studied, too. The immobilized lipase showed somewhat reduced hydrolytic activity, compared to the activity of free lipase as well as in comparison to the lipase immobilized to the reference sample (sample synthesized under the same polymerization conditions, but in the absence of surfactants). It was concluded that the addition of surfactants increased the hydrogel mesh size. The surfactant addition did not affect the dynamic-mechanical properties of the investigated hydrogels. The largest percentage of specific activity and yield of activity were presented by the reference sample, too. It is obvious that the absence of surfactants charged groups has no influence on the lipase binding capacity, and the obtained activity yields are to be expected., U radu je ispitana imobilizacija lipaze iz Candida rugosa u hidrogel poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu nejonskih površinski aktivnih materija. Pri sintezi je varirana vrsta i sadržaj površinski aktivnih materija. Ispitana su mehanička svojstva sintetisanih hidrogelova i urađena je FT-IR analiza. Imobilizacija lipaze je izvedena bubrenjem prethodno sintetisanih hidrogelova u rastvoru lipaze, a zatim je ispitan uticaj dodatka površinski aktivnih materija pri sintezi nosača na kapacitet uklapanja lipaze u hidrogel i na aktivnost tako uklopljene lipaze. Imobilisana lipaza iz Candida rugosa je pokazala umanjenu hidrolitičku aktivnost, kako u odnosu na aktivnost slobodne lipaze, što je i očekivano, tako i u odnosu na aktivnost lipaze imobilisane u referentni hidrogel, sintetisan bez prisustva površinski aktivnih materija.", publisher = "Association of Chemical Engineers of Serbia", journal = "Hemijska industrija", title = "Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants, Imobilizacija lipaze iz Candida rugosa u kopolimerne hidrogelove poli(N-izopropilakrilamid-ko-itakonska kiselina) sintetisane u prisustvu površinski aktivnih materija", pages = "673-667", number = "6", volume = "65", doi = "10.2298/HEMIND110915063M" }
Milašinović, N., Milosavljević, N., Filipović, J. M., Knežević-Jugović, Z.,& Kalagasidis Krušić, M.. (2011). Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants. in Hemijska industrija Association of Chemical Engineers of Serbia., 65(6), 667-673. https://doi.org/10.2298/HEMIND110915063M
Milašinović N, Milosavljević N, Filipović JM, Knežević-Jugović Z, Kalagasidis Krušić M. Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants. in Hemijska industrija. 2011;65(6):667-673. doi:10.2298/HEMIND110915063M .
Milašinović, Nikola, Milosavljević, Nedeljko, Filipović, Jovanka M., Knežević-Jugović, Zorica, Kalagasidis Krušić, Melina, "Immobilization of lipase from Candida rugosa into copolymer hydrogels of poly(N-isopropylacrylamide-co-itaconic acid) synthesized in the presence of surfactants" in Hemijska industrija, 65, no. 6 (2011):667-673, https://doi.org/10.2298/HEMIND110915063M . .