Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase
Samo za registrovane korisnike
2011
Autori
Gvozdenović, MilicaJugović, Branimir
Bezbradica, Dejan
Antov, Mirjana
Knežević-Jugović, Zorica
Grgur, Branimir
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm(-3) HCl and 0.25 mol dm(-3) aniline at constant current density of 2.0 mA cm(-2). Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 mu A cm(-2) were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm(-3) of glucose concentration. The estimated apparent Michaelis-Menten constant, was K-m(') = 0.30 mmol dm(-3), which is significantly lower than that of free enzyme.
Ključne reči:
Glucose oxidase / Immobilisation / Electrochemical synthesis / Polyaniline / BiosensorsIzvor:
Food Chemistry, 2011, 124, 1, 396-400Izdavač:
- Elsevier Sci Ltd, Oxford
Finansiranje / projekti:
- Modifikacija metalnih i nemetalnih materijala elektroprovodnim polimerima za primenu u novim tehnologijama (RS-MESTD-MPN2006-2010-142044)
DOI: 10.1016/j.foodchem.2010.06.046
ISSN: 0308-8146
WoS: 000282500000057
Scopus: 2-s2.0-77955267763
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Gvozdenović, Milica AU - Jugović, Branimir AU - Bezbradica, Dejan AU - Antov, Mirjana AU - Knežević-Jugović, Zorica AU - Grgur, Branimir PY - 2011 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1826 AB - Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm(-3) HCl and 0.25 mol dm(-3) aniline at constant current density of 2.0 mA cm(-2). Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 mu A cm(-2) were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm(-3) of glucose concentration. The estimated apparent Michaelis-Menten constant, was K-m(') = 0.30 mmol dm(-3), which is significantly lower than that of free enzyme. PB - Elsevier Sci Ltd, Oxford T2 - Food Chemistry T1 - Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase EP - 400 IS - 1 SP - 396 VL - 124 DO - 10.1016/j.foodchem.2010.06.046 ER -
@article{ author = "Gvozdenović, Milica and Jugović, Branimir and Bezbradica, Dejan and Antov, Mirjana and Knežević-Jugović, Zorica and Grgur, Branimir", year = "2011", abstract = "Polyaniline (PANI) enzyme electrode was formed by immobilisation of Glucose oxidase (GOx) via glutaraldehyde into electrochemically polymerised PANI on graphite electrode. Electrochemical polymerisation of PANI on graphite was performed from aqueous solution of 1.0 mol dm(-3) HCl and 0.25 mol dm(-3) aniline at constant current density of 2.0 mA cm(-2). Hronopotentiometric curves of the PANI enzyme electrode obtained at current density of 10 mu A cm(-2) were recorded in different glucose concentrations. The linearity response range was between 1.0 and 5.0 mmol dm(-3) of glucose concentration. The estimated apparent Michaelis-Menten constant, was K-m(') = 0.30 mmol dm(-3), which is significantly lower than that of free enzyme.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Food Chemistry", title = "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase", pages = "400-396", number = "1", volume = "124", doi = "10.1016/j.foodchem.2010.06.046" }
Gvozdenović, M., Jugović, B., Bezbradica, D., Antov, M., Knežević-Jugović, Z.,& Grgur, B.. (2011). Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry Elsevier Sci Ltd, Oxford., 124(1), 396-400. https://doi.org/10.1016/j.foodchem.2010.06.046
Gvozdenović M, Jugović B, Bezbradica D, Antov M, Knežević-Jugović Z, Grgur B. Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase. in Food Chemistry. 2011;124(1):396-400. doi:10.1016/j.foodchem.2010.06.046 .
Gvozdenović, Milica, Jugović, Branimir, Bezbradica, Dejan, Antov, Mirjana, Knežević-Jugović, Zorica, Grgur, Branimir, "Electrochemical determination of glucose using polyaniline electrode modified by glucose oxidase" in Food Chemistry, 124, no. 1 (2011):396-400, https://doi.org/10.1016/j.foodchem.2010.06.046 . .