Micro-environmentally restricted hybridoma cell growth within polysaccharide hydrogel microbeads
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2013
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The mechanism of micro-environmentally restricted hybridoma cell growth caused by action of local mechanical compression stress generated within various polysaccharide hydrogel matrixes is estimated by comparing the growth of hybridoma cells within (1) 1.5% Ca-alginate microbeads from Bugarski et al. [in: Fundamentals of Animal Cells Immobilization and Microencapsulation, M. F. A. Goosen, ed., CRC Press, Boca Raton, FL, 1993, p. 267] and (2) 1.3% alginate-agarose microbeads from Shen et al. [Animal Cell Technology: Basic & Applied Aspects, H. Murakami ed., Kluwer Academic Publishers, The Netherlands, 1992, p. 173]. Consideration of restricted cell growth dynamics based on developed kinetic model and kinetic 3D Monte Carlo simulation include: (1) changes the fraction of active proliferating cells in the exponential phase and (2) changes of non-proliferating cell concentration in the plateau phase. Higher value of the specific decrease of active fraction of proliferating cells. is obtain...ed for 1.3% alginate-agarose compared to 1.5% alginate microbeads. It corresponds to higher compression stress generated within hydrogel matrix during cell growth obtained for 1.3% alginate-agarose microbeads.
Ključne reči:
Immobilized cells / hydrogel / local compression stress / bioprocess design / modelingIzvor:
Bio-Medical Materials and Engineering, 2013, 23, 5, 361-371Izdavač:
- IOS Press, Amsterdam
Finansiranje / projekti:
- Razvoj i primena novih i tradicionalnih tehnologija u proizvodnji konkurentnih prehrambenih proizvoda sa dodatom vrednošću za evropsko i svetsko tržište - Stvorimo bogatstvo iz bogatstva Srbije (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46001)
DOI: 10.3233/BME-130760
ISSN: 0959-2989
PubMed: 23988708
WoS: 000323845500005
Scopus: 2-s2.0-84883704482
Institucija/grupa
Tehnološko-metalurški fakultetTY - JOUR AU - Pajić-Lijaković, Ivana PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2394 AB - The mechanism of micro-environmentally restricted hybridoma cell growth caused by action of local mechanical compression stress generated within various polysaccharide hydrogel matrixes is estimated by comparing the growth of hybridoma cells within (1) 1.5% Ca-alginate microbeads from Bugarski et al. [in: Fundamentals of Animal Cells Immobilization and Microencapsulation, M. F. A. Goosen, ed., CRC Press, Boca Raton, FL, 1993, p. 267] and (2) 1.3% alginate-agarose microbeads from Shen et al. [Animal Cell Technology: Basic & Applied Aspects, H. Murakami ed., Kluwer Academic Publishers, The Netherlands, 1992, p. 173]. Consideration of restricted cell growth dynamics based on developed kinetic model and kinetic 3D Monte Carlo simulation include: (1) changes the fraction of active proliferating cells in the exponential phase and (2) changes of non-proliferating cell concentration in the plateau phase. Higher value of the specific decrease of active fraction of proliferating cells. is obtained for 1.3% alginate-agarose compared to 1.5% alginate microbeads. It corresponds to higher compression stress generated within hydrogel matrix during cell growth obtained for 1.3% alginate-agarose microbeads. PB - IOS Press, Amsterdam T2 - Bio-Medical Materials and Engineering T1 - Micro-environmentally restricted hybridoma cell growth within polysaccharide hydrogel microbeads EP - 371 IS - 5 SP - 361 VL - 23 DO - 10.3233/BME-130760 ER -
@article{ author = "Pajić-Lijaković, Ivana", year = "2013", abstract = "The mechanism of micro-environmentally restricted hybridoma cell growth caused by action of local mechanical compression stress generated within various polysaccharide hydrogel matrixes is estimated by comparing the growth of hybridoma cells within (1) 1.5% Ca-alginate microbeads from Bugarski et al. [in: Fundamentals of Animal Cells Immobilization and Microencapsulation, M. F. A. Goosen, ed., CRC Press, Boca Raton, FL, 1993, p. 267] and (2) 1.3% alginate-agarose microbeads from Shen et al. [Animal Cell Technology: Basic & Applied Aspects, H. Murakami ed., Kluwer Academic Publishers, The Netherlands, 1992, p. 173]. Consideration of restricted cell growth dynamics based on developed kinetic model and kinetic 3D Monte Carlo simulation include: (1) changes the fraction of active proliferating cells in the exponential phase and (2) changes of non-proliferating cell concentration in the plateau phase. Higher value of the specific decrease of active fraction of proliferating cells. is obtained for 1.3% alginate-agarose compared to 1.5% alginate microbeads. It corresponds to higher compression stress generated within hydrogel matrix during cell growth obtained for 1.3% alginate-agarose microbeads.", publisher = "IOS Press, Amsterdam", journal = "Bio-Medical Materials and Engineering", title = "Micro-environmentally restricted hybridoma cell growth within polysaccharide hydrogel microbeads", pages = "371-361", number = "5", volume = "23", doi = "10.3233/BME-130760" }
Pajić-Lijaković, I.. (2013). Micro-environmentally restricted hybridoma cell growth within polysaccharide hydrogel microbeads. in Bio-Medical Materials and Engineering IOS Press, Amsterdam., 23(5), 361-371. https://doi.org/10.3233/BME-130760
Pajić-Lijaković I. Micro-environmentally restricted hybridoma cell growth within polysaccharide hydrogel microbeads. in Bio-Medical Materials and Engineering. 2013;23(5):361-371. doi:10.3233/BME-130760 .
Pajić-Lijaković, Ivana, "Micro-environmentally restricted hybridoma cell growth within polysaccharide hydrogel microbeads" in Bio-Medical Materials and Engineering, 23, no. 5 (2013):361-371, https://doi.org/10.3233/BME-130760 . .