Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116
Authors
Obradović, AnaZizić, Jovana
Trišović, Nemanja
Božić, Bojan
Ušćumlić, Gordana
Božić, Biljana
Marković, Snežana
Article (Published version)
Metadata
Show full item recordAbstract
Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.
Keywords:
Antioxidants / phenytoin derivatives / free radicals / redox homeostasisSource:
Turkish Journal of Biology, 2013, 37, 6, 741-747Publisher:
- Tubitak Scientific & Technical Research Council Turkey, Ankara
Funding / projects:
- Preclinical investigation of bioactive substances (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41010)
- Study of the Synthesis, Structure and Activity of Natural and Synthetic Organic Compounds (RS-MESTD-Basic Research (BR or ON)-172013)
DOI: 10.3906/biy-1302-15
ISSN: 1300-0152
WoS: 000325747400014
Scopus: 2-s2.0-84885199159
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Obradović, Ana AU - Zizić, Jovana AU - Trišović, Nemanja AU - Božić, Bojan AU - Ušćumlić, Gordana AU - Božić, Biljana AU - Marković, Snežana PY - 2013 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/2427 AB - Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production. PB - Tubitak Scientific & Technical Research Council Turkey, Ankara T2 - Turkish Journal of Biology T1 - Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116 EP - 747 IS - 6 SP - 741 VL - 37 DO - 10.3906/biy-1302-15 ER -
@article{ author = "Obradović, Ana and Zizić, Jovana and Trišović, Nemanja and Božić, Bojan and Ušćumlić, Gordana and Božić, Biljana and Marković, Snežana", year = "2013", abstract = "Antioxidant effects of twelve 3-substituted-5,5-diphenylhydantoins in concentrations of 0.01, 0.1, 1, 10, and 100 mu M on human colon cancer cell line HCT-116 were determined 24 h after treatment. The concentrations of superoxide anion radical (O(2)radical anion), nitrites (NO2-), and total glutathione were determined spectrophotometrically. Results indicated that treatment with all compounds induced a decrease in O(2)radical anion and an increase in NO(2)(-)concentrations in HCT-116 cells. Treatment with all compounds resulted in an increase in glutathione, and this indicates changes in redox homeostasis. Based on the obtained results, the investigated hydantoins act as antioxidants because they decrease the production of superoxide anion radical and increase concentrations of glutathione, but they also induce an increase in nitrite production.", publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara", journal = "Turkish Journal of Biology", title = "Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116", pages = "747-741", number = "6", volume = "37", doi = "10.3906/biy-1302-15" }
Obradović, A., Zizić, J., Trišović, N., Božić, B., Ušćumlić, G., Božić, B.,& Marković, S.. (2013). Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116. in Turkish Journal of Biology Tubitak Scientific & Technical Research Council Turkey, Ankara., 37(6), 741-747. https://doi.org/10.3906/biy-1302-15
Obradović A, Zizić J, Trišović N, Božić B, Ušćumlić G, Božić B, Marković S. Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116. in Turkish Journal of Biology. 2013;37(6):741-747. doi:10.3906/biy-1302-15 .
Obradović, Ana, Zizić, Jovana, Trišović, Nemanja, Božić, Bojan, Ušćumlić, Gordana, Božić, Biljana, Marković, Snežana, "Evaluation of antioxidative effects of twelve 3-substituted-5,5-diphenylhydantoins on human colon cancer cell line HCT-116" in Turkish Journal of Biology, 37, no. 6 (2013):741-747, https://doi.org/10.3906/biy-1302-15 . .