Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses
Само за регистроване кориснике
2016
Аутори
Miljković, MionaDavidović, Slađana
Simović, Milica
Veljović, Đorđe
Mladenović, Dragana
Rajilić-Stojanović, Mirjana
Dimitrijević-Branković, Suzana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated... SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product.
Кључне речи:
Molasses / Sugar beep pulp / Leuconostoc mesenteroides T3 / Immobilization / DextransucraseИзвор:
Applied Biochemistry and Biotechnology, 2016, 180, 5, 1016-1027Издавач:
- Humana Press Inc, Totowa
Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
DOI: 10.1007/s12010-016-2149-x
ISSN: 0273-2289
PubMed: 27287996
WoS: 000387540700015
Scopus: 2-s2.0-84973620842
Колекције
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Miljković, Miona AU - Davidović, Slađana AU - Simović, Milica AU - Veljović, Đorđe AU - Mladenović, Dragana AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2016 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3290 AB - Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product. PB - Humana Press Inc, Totowa T2 - Applied Biochemistry and Biotechnology T1 - Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses EP - 1027 IS - 5 SP - 1016 VL - 180 DO - 10.1007/s12010-016-2149-x ER -
@article{ author = "Miljković, Miona and Davidović, Slađana and Simović, Milica and Veljović, Đorđe and Mladenović, Dragana and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2016", abstract = "Sugar beet pulp (SBP) and molasses, as an agro industrial waste material, are produced in large amounts annually. Thus, a major challenge nowadays is to develop procedures that could increase the value of the generated waste. In this study, SBP as a support for cell immobilization and molasses as a source of nutrients were used for a dextransucrase (DS) production by Leuconostoc mesenteroides T3. The influence of SBP in native form (SBP-N) and after treatment with NaOH (SBP-NaOH) on DS production was investigated. The optimal medium composition for the maximum DS production was determined by varying the concentration of molasses, SBP, and sucrose. The maximum DS yield of 2.02 U/ml was obtained in the medium with 2.5 % of molasses, 2.5 % SBP-NaOH, and 4 % of sucrose concentration. Scanning electron microscopy (SEM) showed immobilization of Lc. mesenteroides T3 cells onto SBP-NaOH. According to the obtained results, the production of DS on molasses could be improved by using NaOH-treated SBP as a carrier for whole-cell immobilization. Our study reveals the basis for the development of process for DS production with additional reduction of expenses by using waste materials for obtaining the valuable biotechnological product.", publisher = "Humana Press Inc, Totowa", journal = "Applied Biochemistry and Biotechnology", title = "Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses", pages = "1027-1016", number = "5", volume = "180", doi = "10.1007/s12010-016-2149-x" }
Miljković, M., Davidović, S., Simović, M., Veljović, Đ., Mladenović, D., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2016). Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses. in Applied Biochemistry and Biotechnology Humana Press Inc, Totowa., 180(5), 1016-1027. https://doi.org/10.1007/s12010-016-2149-x
Miljković M, Davidović S, Simović M, Veljović Đ, Mladenović D, Rajilić-Stojanović M, Dimitrijević-Branković S. Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses. in Applied Biochemistry and Biotechnology. 2016;180(5):1016-1027. doi:10.1007/s12010-016-2149-x .
Miljković, Miona, Davidović, Slađana, Simović, Milica, Veljović, Đorđe, Mladenović, Dragana, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Sugar Beet Pulp as Leuconostoc mesenteroides T3 Support for Enhanced Dextransucrase Production on Molasses" in Applied Biochemistry and Biotechnology, 180, no. 5 (2016):1016-1027, https://doi.org/10.1007/s12010-016-2149-x . .