Covalent immobilization of enzymes on Eupergit® supports: Effect of the immobilization protocol
Authorized Users Only
2017
Authors
Knežević-Jugović, ZoricaGrbavčić, Sanja
Jovanović, Jelena
Stefanović, Andrea
Bezbradica, Dejan
Mijin, Dušan
Antov, Mirjana
Article (Published version)
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Show full item recordAbstract
A selection of best combination of adequate immobilization support and efficient immobilization method is still a key requirement for successful application of immobilized enzymes on an industrial level. Eupergit® supports exhibit good mechanical and chemical properties and allow establishment of satisfactory hydrodynamic regime in enzyme reactors. This is advantageous for their wide application in enzyme immobilization after finding the most favorable immobilization method. Methods for enzyme immobilization that have been previously reported as efficient considering the obtained activity of immobilized enzyme are presented: direct binding to polymers via their epoxy groups, binding to polymers via a spacer made from ethylene diamine/glutaraldehyde, and coupling the periodate-oxidized sugar moieties of the enzymes to the polymer beads. The modification of the conventionally immobilized enzyme with ethylenediamine via the carbodiimide route seems to be a powerful tool to improve its sta...bility and catalytic activity.
Keywords:
Aminated support / Carbodiimide chemistry / Covalent immobilization / Enzyme chemical amination / Epoxy groups / Eupergit C / Glycoenzymes / Multipoint covalent attachment / Periodate oxidationSource:
Methods in Molecular Biology, 2017, 1504, 75-91Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1007/978-1-4939-6499-4_7
ISSN: 1064-3745
PubMed:
Scopus: 2-s2.0-84992457812
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Knežević-Jugović, Zorica AU - Grbavčić, Sanja AU - Jovanović, Jelena AU - Stefanović, Andrea AU - Bezbradica, Dejan AU - Mijin, Dušan AU - Antov, Mirjana PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3499 AB - A selection of best combination of adequate immobilization support and efficient immobilization method is still a key requirement for successful application of immobilized enzymes on an industrial level. Eupergit® supports exhibit good mechanical and chemical properties and allow establishment of satisfactory hydrodynamic regime in enzyme reactors. This is advantageous for their wide application in enzyme immobilization after finding the most favorable immobilization method. Methods for enzyme immobilization that have been previously reported as efficient considering the obtained activity of immobilized enzyme are presented: direct binding to polymers via their epoxy groups, binding to polymers via a spacer made from ethylene diamine/glutaraldehyde, and coupling the periodate-oxidized sugar moieties of the enzymes to the polymer beads. The modification of the conventionally immobilized enzyme with ethylenediamine via the carbodiimide route seems to be a powerful tool to improve its stability and catalytic activity. T2 - Methods in Molecular Biology T1 - Covalent immobilization of enzymes on Eupergit® supports: Effect of the immobilization protocol EP - 91 SP - 75 VL - 1504 DO - 10.1007/978-1-4939-6499-4_7 ER -
@article{ author = "Knežević-Jugović, Zorica and Grbavčić, Sanja and Jovanović, Jelena and Stefanović, Andrea and Bezbradica, Dejan and Mijin, Dušan and Antov, Mirjana", year = "2017", abstract = "A selection of best combination of adequate immobilization support and efficient immobilization method is still a key requirement for successful application of immobilized enzymes on an industrial level. Eupergit® supports exhibit good mechanical and chemical properties and allow establishment of satisfactory hydrodynamic regime in enzyme reactors. This is advantageous for their wide application in enzyme immobilization after finding the most favorable immobilization method. Methods for enzyme immobilization that have been previously reported as efficient considering the obtained activity of immobilized enzyme are presented: direct binding to polymers via their epoxy groups, binding to polymers via a spacer made from ethylene diamine/glutaraldehyde, and coupling the periodate-oxidized sugar moieties of the enzymes to the polymer beads. The modification of the conventionally immobilized enzyme with ethylenediamine via the carbodiimide route seems to be a powerful tool to improve its stability and catalytic activity.", journal = "Methods in Molecular Biology", title = "Covalent immobilization of enzymes on Eupergit® supports: Effect of the immobilization protocol", pages = "91-75", volume = "1504", doi = "10.1007/978-1-4939-6499-4_7" }
Knežević-Jugović, Z., Grbavčić, S., Jovanović, J., Stefanović, A., Bezbradica, D., Mijin, D.,& Antov, M.. (2017). Covalent immobilization of enzymes on Eupergit® supports: Effect of the immobilization protocol. in Methods in Molecular Biology, 1504, 75-91. https://doi.org/10.1007/978-1-4939-6499-4_7
Knežević-Jugović Z, Grbavčić S, Jovanović J, Stefanović A, Bezbradica D, Mijin D, Antov M. Covalent immobilization of enzymes on Eupergit® supports: Effect of the immobilization protocol. in Methods in Molecular Biology. 2017;1504:75-91. doi:10.1007/978-1-4939-6499-4_7 .
Knežević-Jugović, Zorica, Grbavčić, Sanja, Jovanović, Jelena, Stefanović, Andrea, Bezbradica, Dejan, Mijin, Dušan, Antov, Mirjana, "Covalent immobilization of enzymes on Eupergit® supports: Effect of the immobilization protocol" in Methods in Molecular Biology, 1504 (2017):75-91, https://doi.org/10.1007/978-1-4939-6499-4_7 . .