Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak
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Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Apples and sweet cherries are generally characterized by self-incompatibility, which is controlled by the multiallelic locus S with gametophytic action. Thus the identification of S-genotype represents crucial information for fruit breeders and growers. The aim of this study was to identify the S-genotype of two apple ('Cacanska Pozna' and 'Cadel') and two sweet cherry ('Asenova Rana' and 'Carna') cultivars developed at Fruit Research Institute, Cacak using the polymerase chain reaction (PCR) method with consensus and allele-specific primers. The S-genotype of apple cultivars 'Cacanska Pozna' (S7S9) and 'Cadel' (S2S7) were consistent with parental combinations 'Starking Delicious' (S9S28) x 'Jonathan' (S7S9) and 'Golden Delicious' (S2S3) x 'Jonathan' (S7S9), respectively. For sweet cherry cultivars 'Asenova Rana' ['Drogans Gelbe' (S1S5) x 'Majova Rana' (S1S4)] and 'Carna' ['Majova Rana' (S1S4) x 'Bigarreau de Schrecken' (S1S3)], S3S9 and S1S4 were identified respectively, suggesting th...at the reported pedigree is erroneous. The trueness-to-type of DNA samples of both sweet cherry cultivars was confirmed by comparison of cultivar samples from more than one site. Upon this testing, the remaining inconsistency indicated that aforementioned parentages of the sweet cherry cultivars were incorrect. These discrepancies could be the result of possible pollen contamination, or mistakes either in breeders recording or in seed processing and seedling production. The results of S-genotyping of apple and sweet cherry cultivars provide relevant information on correct assignment of these cultivars to cross-compatibility groups, which are important for cross design in developing new cultivar, as well as for orchard management in the efficient production of fruits.
Ključne reči:
Malus x domestica / Prunus avium / planned hybridisation / self-incompatibility / PCR amplificationIzvor:
GENETIKA-BELGRADE, 2017, 49, 1, 127-138Izdavač:
- Društvo genetičara Srbije, Beograd
Finansiranje / projekti:
- Stvaranje i očuvanje genetičkog potencijala kontinentalnih vrsta voćaka (RS-MESTD-Technological Development (TD or TR)-31064)
DOI: 10.2298/GENSR1701127M
ISSN: 0534-0012
WoS: 000405684200012
Scopus: 2-s2.0-85026199502
Institucija/grupa
Inovacioni centarTY - JOUR AU - Marić, Slađana AU - Radičević, Sanja AU - Lukić, Milan AU - Cerović, Radosav AU - Paunović, Svetlana A. PY - 2017 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3572 AB - Apples and sweet cherries are generally characterized by self-incompatibility, which is controlled by the multiallelic locus S with gametophytic action. Thus the identification of S-genotype represents crucial information for fruit breeders and growers. The aim of this study was to identify the S-genotype of two apple ('Cacanska Pozna' and 'Cadel') and two sweet cherry ('Asenova Rana' and 'Carna') cultivars developed at Fruit Research Institute, Cacak using the polymerase chain reaction (PCR) method with consensus and allele-specific primers. The S-genotype of apple cultivars 'Cacanska Pozna' (S7S9) and 'Cadel' (S2S7) were consistent with parental combinations 'Starking Delicious' (S9S28) x 'Jonathan' (S7S9) and 'Golden Delicious' (S2S3) x 'Jonathan' (S7S9), respectively. For sweet cherry cultivars 'Asenova Rana' ['Drogans Gelbe' (S1S5) x 'Majova Rana' (S1S4)] and 'Carna' ['Majova Rana' (S1S4) x 'Bigarreau de Schrecken' (S1S3)], S3S9 and S1S4 were identified respectively, suggesting that the reported pedigree is erroneous. The trueness-to-type of DNA samples of both sweet cherry cultivars was confirmed by comparison of cultivar samples from more than one site. Upon this testing, the remaining inconsistency indicated that aforementioned parentages of the sweet cherry cultivars were incorrect. These discrepancies could be the result of possible pollen contamination, or mistakes either in breeders recording or in seed processing and seedling production. The results of S-genotyping of apple and sweet cherry cultivars provide relevant information on correct assignment of these cultivars to cross-compatibility groups, which are important for cross design in developing new cultivar, as well as for orchard management in the efficient production of fruits. PB - Društvo genetičara Srbije, Beograd T2 - GENETIKA-BELGRADE T1 - Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak EP - 138 IS - 1 SP - 127 VL - 49 DO - 10.2298/GENSR1701127M ER -
@article{ author = "Marić, Slađana and Radičević, Sanja and Lukić, Milan and Cerović, Radosav and Paunović, Svetlana A.", year = "2017", abstract = "Apples and sweet cherries are generally characterized by self-incompatibility, which is controlled by the multiallelic locus S with gametophytic action. Thus the identification of S-genotype represents crucial information for fruit breeders and growers. The aim of this study was to identify the S-genotype of two apple ('Cacanska Pozna' and 'Cadel') and two sweet cherry ('Asenova Rana' and 'Carna') cultivars developed at Fruit Research Institute, Cacak using the polymerase chain reaction (PCR) method with consensus and allele-specific primers. The S-genotype of apple cultivars 'Cacanska Pozna' (S7S9) and 'Cadel' (S2S7) were consistent with parental combinations 'Starking Delicious' (S9S28) x 'Jonathan' (S7S9) and 'Golden Delicious' (S2S3) x 'Jonathan' (S7S9), respectively. For sweet cherry cultivars 'Asenova Rana' ['Drogans Gelbe' (S1S5) x 'Majova Rana' (S1S4)] and 'Carna' ['Majova Rana' (S1S4) x 'Bigarreau de Schrecken' (S1S3)], S3S9 and S1S4 were identified respectively, suggesting that the reported pedigree is erroneous. The trueness-to-type of DNA samples of both sweet cherry cultivars was confirmed by comparison of cultivar samples from more than one site. Upon this testing, the remaining inconsistency indicated that aforementioned parentages of the sweet cherry cultivars were incorrect. These discrepancies could be the result of possible pollen contamination, or mistakes either in breeders recording or in seed processing and seedling production. The results of S-genotyping of apple and sweet cherry cultivars provide relevant information on correct assignment of these cultivars to cross-compatibility groups, which are important for cross design in developing new cultivar, as well as for orchard management in the efficient production of fruits.", publisher = "Društvo genetičara Srbije, Beograd", journal = "GENETIKA-BELGRADE", title = "Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak", pages = "138-127", number = "1", volume = "49", doi = "10.2298/GENSR1701127M" }
Marić, S., Radičević, S., Lukić, M., Cerović, R.,& Paunović, S. A.. (2017). Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak. in GENETIKA-BELGRADE Društvo genetičara Srbije, Beograd., 49(1), 127-138. https://doi.org/10.2298/GENSR1701127M
Marić S, Radičević S, Lukić M, Cerović R, Paunović SA. Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak. in GENETIKA-BELGRADE. 2017;49(1):127-138. doi:10.2298/GENSR1701127M .
Marić, Slađana, Radičević, Sanja, Lukić, Milan, Cerović, Radosav, Paunović, Svetlana A., "Determination of s-genotype in apple and sweet cherry cultivars released at fruit research institute, Cacak" in GENETIKA-BELGRADE, 49, no. 1 (2017):127-138, https://doi.org/10.2298/GENSR1701127M . .