dc.creator | Drvenica, Ivana | |
dc.creator | Stančić, Ana | |
dc.creator | Kalušević, Ana | |
dc.creator | Marković, Smilja | |
dc.creator | Dragišić-Maksimović, Jelena | |
dc.creator | Nedović, Viktor | |
dc.creator | Bugarski, Branko | |
dc.creator | Ilić, Vesna Lj. | |
dc.date.accessioned | 2021-03-10T13:59:26Z | |
dc.date.available | 2021-03-10T13:59:26Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4135 | |
dc.description.abstract | Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization. | en |
dc.publisher | Srpsko hemijsko društvo, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | slaughterhouse blood | en |
dc.subject | heme-iron protein | en |
dc.subject | dynamic light scattering | en |
dc.subject | UV-Vis spectroscopy | en |
dc.title | Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 1117 | |
dc.citation.issue | 10 | |
dc.citation.other | 84(10): 1105-1117 | |
dc.citation.rank | M23 | |
dc.citation.spage | 1105 | |
dc.citation.volume | 84 | |
dc.identifier.doi | 10.2298/JSC190513067D | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/1852/4132.pdf | |
dc.identifier.scopus | 2-s2.0-85074904247 | |
dc.identifier.wos | 000496149600005 | |
dc.type.version | publishedVersion | |