dc.creator | Mladenović, Dragana | |
dc.creator | Pejin, Jelena | |
dc.creator | Kocić-Tanackov, Sunčica | |
dc.creator | Đukić-Vuković, Aleksandra | |
dc.creator | Mojović, Ljiljana | |
dc.date.accessioned | 2021-03-10T14:05:11Z | |
dc.date.available | 2021-03-10T14:05:11Z | |
dc.date.issued | 2019 | |
dc.identifier.issn | 0273-2289 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4224 | |
dc.description.abstract | The aim of this study was to perform the adaptation of Lactobacillus paracasei NRRL B-4564 to substrate through adaptive evolution in order to ensure intensive substrate utilization and enhanced L (+)-lactic acid (LA) production on molasses-enriched potato stillage. To evaluate the strain response to environmental conditions exposed during the adaptation process and to select the best adapted cells, the antioxidant activity and LA-producing capability were assessed in batch fermentation. The most promising adapted strain was further used in a pulsed fed-batch mode. Among three selected adapted strains, L. paracasei A-22 showed considerably improved antioxidant capacity, demonstrating more than onefold higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging rates compared to parent strain. This strain also exhibited superior LA production in batch fermentation and reached 89.4gL(-1) of LA, with a yield of 0.89gg(-1), a productivity of 1.49gL(-1)h(-1), and an optical purity greater than 99%. Furthermore, in fed-batch mode L. paracasei A-22 resulted in 59% higher LA concentration (169.9gL(-1)) compared to parent strain (107.1gL(-1)). The strain adaptation to molasses environment, performed in this study, is a rather simple and promising method for enhancement of LA production on the complex agro-industrial substrate. | en |
dc.publisher | Springer, New York | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31017/RS// | |
dc.rights | restrictedAccess | |
dc.source | Applied Biochemistry and Biotechnology | |
dc.subject | Strain adaptation | en |
dc.subject | Antioxidant activity | en |
dc.subject | Lactic acid | en |
dc.subject | Fed-batch fermentation | en |
dc.subject | Sugar beet molasses | en |
dc.subject | Potato stillage | en |
dc.title | Enhanced Lactic Acid Production by Adaptive Evolution of Lactobacillus paracasei on Agro-industrial Substrate | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 769 | |
dc.citation.issue | 3 | |
dc.citation.other | 187(3): 753-769 | |
dc.citation.rank | M22 | |
dc.citation.spage | 753 | |
dc.citation.volume | 187 | |
dc.identifier.doi | 10.1007/s12010-018-2852-x | |
dc.identifier.pmid | 30058018 | |
dc.identifier.scopus | 2-s2.0-85050955692 | |
dc.identifier.wos | 000463838900007 | |
dc.type.version | publishedVersion | |