Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor
Autori
Svetozarević, MilicaŠekuljica, Nataša
Onjia, Antonije
Barać, Nemanja
Mihajlović, Marina
Knežević-Jugović, Zorica
Mijin, Dušan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This paper aims to reduce the gap between intensified innovative process designs and the application of enzyme technologies at an industrial scale that often take full advantage of microreactor systems. Soybean and potato processing waste was used as the source of peroxidase in both the free and immobilized form for the biodegradation of the Acid Violet 109 anthraquinone dye (AV109) in a PTFE (polytetrafluoroethylene) microreactor which is 0.5 mm in diameter and 6 m in length. High biodegradation efficiencies of 95 and 76% were achieved by soybean and potato peroxidase respectively after 3 min of residence in the microreactor, with a noticeable lack of hydrogen peroxide inhibition. The reactor’s design, with regard to its diameter and length, significantly affected the biodegradation efficiency. The polymer membrane formation by the cross-linking approach with oxidized pectin and soybean or potato peel peroxidase on the inner PTFE wall was SEM confirmed. The cross-linking efficiency wi...th oxidized pectin of both peroxidases is inversely correlated with the activity, hence the highest recorded activity of immobilized peroxidase was 11 and 19μmol/min of soybean and potato peroxidase, respectively, at an initial pectin concentration of 0.05%. A promising reusability for AV109 biodegradation in the microreactor with immobilized soybean and potato peroxidase was confirmed, since the immobilized peroxidases retained 65 and 35% of the initial activity after the tenth cycle, respectively. The LC–MS and GC–MS techniques confirmed the biodegradation of the AV 109 dye, and the intermediates with a lower molecular weight were separated and identified.
Ključne reči:
Anthraquinone dye / Biodegradation / Immobilization / Microreactor / Potato peroxidase / Soybean peroxidaseIzvor:
Environmental Technology & Innovation, 2022, 26, 102373-Izdavač:
- Elsevier B.V.
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200287 (Inovacioni centar Tehnološko-metalurškog fakulteta u Beogradu doo) (RS-MESTD-inst-2020-200287)
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200135 (Univerzitet u Beogradu, Tehnološko-metalurški fakultet) (RS-MESTD-inst-2020-200135)
Napomena:
- Supplementary material: http://technorep.tmf.bg.ac.rs/handle/123456789/4730
Povezane informacije:
DOI: 10.1016/j.eti.2022.102373
ISSN: 2352-1864
WoS: 00083287670000
Scopus: 2-s2.0-85124697703
Kolekcije
Institucija/grupa
Inovacioni centarTY - JOUR AU - Svetozarević, Milica AU - Šekuljica, Nataša AU - Onjia, Antonije AU - Barać, Nemanja AU - Mihajlović, Marina AU - Knežević-Jugović, Zorica AU - Mijin, Dušan PY - 2022 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4729 AB - This paper aims to reduce the gap between intensified innovative process designs and the application of enzyme technologies at an industrial scale that often take full advantage of microreactor systems. Soybean and potato processing waste was used as the source of peroxidase in both the free and immobilized form for the biodegradation of the Acid Violet 109 anthraquinone dye (AV109) in a PTFE (polytetrafluoroethylene) microreactor which is 0.5 mm in diameter and 6 m in length. High biodegradation efficiencies of 95 and 76% were achieved by soybean and potato peroxidase respectively after 3 min of residence in the microreactor, with a noticeable lack of hydrogen peroxide inhibition. The reactor’s design, with regard to its diameter and length, significantly affected the biodegradation efficiency. The polymer membrane formation by the cross-linking approach with oxidized pectin and soybean or potato peel peroxidase on the inner PTFE wall was SEM confirmed. The cross-linking efficiency with oxidized pectin of both peroxidases is inversely correlated with the activity, hence the highest recorded activity of immobilized peroxidase was 11 and 19μmol/min of soybean and potato peroxidase, respectively, at an initial pectin concentration of 0.05%. A promising reusability for AV109 biodegradation in the microreactor with immobilized soybean and potato peroxidase was confirmed, since the immobilized peroxidases retained 65 and 35% of the initial activity after the tenth cycle, respectively. The LC–MS and GC–MS techniques confirmed the biodegradation of the AV 109 dye, and the intermediates with a lower molecular weight were separated and identified. PB - Elsevier B.V. T2 - Environmental Technology & Innovation T1 - Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor SP - 102373 VL - 26 DO - 10.1016/j.eti.2022.102373 ER -
@article{ author = "Svetozarević, Milica and Šekuljica, Nataša and Onjia, Antonije and Barać, Nemanja and Mihajlović, Marina and Knežević-Jugović, Zorica and Mijin, Dušan", year = "2022", abstract = "This paper aims to reduce the gap between intensified innovative process designs and the application of enzyme technologies at an industrial scale that often take full advantage of microreactor systems. Soybean and potato processing waste was used as the source of peroxidase in both the free and immobilized form for the biodegradation of the Acid Violet 109 anthraquinone dye (AV109) in a PTFE (polytetrafluoroethylene) microreactor which is 0.5 mm in diameter and 6 m in length. High biodegradation efficiencies of 95 and 76% were achieved by soybean and potato peroxidase respectively after 3 min of residence in the microreactor, with a noticeable lack of hydrogen peroxide inhibition. The reactor’s design, with regard to its diameter and length, significantly affected the biodegradation efficiency. The polymer membrane formation by the cross-linking approach with oxidized pectin and soybean or potato peel peroxidase on the inner PTFE wall was SEM confirmed. The cross-linking efficiency with oxidized pectin of both peroxidases is inversely correlated with the activity, hence the highest recorded activity of immobilized peroxidase was 11 and 19μmol/min of soybean and potato peroxidase, respectively, at an initial pectin concentration of 0.05%. A promising reusability for AV109 biodegradation in the microreactor with immobilized soybean and potato peroxidase was confirmed, since the immobilized peroxidases retained 65 and 35% of the initial activity after the tenth cycle, respectively. The LC–MS and GC–MS techniques confirmed the biodegradation of the AV 109 dye, and the intermediates with a lower molecular weight were separated and identified.", publisher = "Elsevier B.V.", journal = "Environmental Technology & Innovation", title = "Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor", pages = "102373", volume = "26", doi = "10.1016/j.eti.2022.102373" }
Svetozarević, M., Šekuljica, N., Onjia, A., Barać, N., Mihajlović, M., Knežević-Jugović, Z.,& Mijin, D.. (2022). Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor. in Environmental Technology & Innovation Elsevier B.V.., 26, 102373. https://doi.org/10.1016/j.eti.2022.102373
Svetozarević M, Šekuljica N, Onjia A, Barać N, Mihajlović M, Knežević-Jugović Z, Mijin D. Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor. in Environmental Technology & Innovation. 2022;26:102373. doi:10.1016/j.eti.2022.102373 .
Svetozarević, Milica, Šekuljica, Nataša, Onjia, Antonije, Barać, Nemanja, Mihajlović, Marina, Knežević-Jugović, Zorica, Mijin, Dušan, "Biodegradation of synthetic dyes by free and cross-linked peroxidase in microfluidic reactor" in Environmental Technology & Innovation, 26 (2022):102373, https://doi.org/10.1016/j.eti.2022.102373 . .