Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3
Само за регистроване кориснике
2021
Аутори
Mihajlovski, KatarinaPecarski, Danijela
Rajilić-Stojanović, Mirjana
Dimitrijević-Branković, Suzana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under... optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.
Кључне речи:
Hymenobacter / Enzymes / Lignocellulosic hydrolysis / Bioethanol / OptimizationИзвор:
Environmental Technology & Innovation, 2021, 23Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
DOI: 10.1016/j.eti.2021.101627
ISSN: 2352-1864
WoS: 000685036700004
Scopus: 2-s2.0-85106219526
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Mihajlovski, Katarina AU - Pecarski, Danijela AU - Rajilić-Stojanović, Mirjana AU - Dimitrijević-Branković, Suzana PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4827 AB - During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production. T2 - Environmental Technology & Innovation T1 - Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3 VL - 23 DO - 10.1016/j.eti.2021.101627 ER -
@article{ author = "Mihajlovski, Katarina and Pecarski, Danijela and Rajilić-Stojanović, Mirjana and Dimitrijević-Branković, Suzana", year = "2021", abstract = "During the last few decades, energy demand is increasing rapidly. Concerning this, the use of renewables - lignocellulose biomass, for bioethanol production, as an efficient alternative to replacing fossil fuels, is highly recommended. In this study, valorization of two agricultural wastes was used for various hydrolytic enzyme production by Hymenobacter sp. CKS3, lignocellulosic hydrolysis, and bioethanol production. Conditions for obtaining maximum enzyme production, using agro-industrial waste - molasses and corn stover, were statistically optimized. Under the optimal conditions, in a medium containing 5.0% corn stover, 2.5% molasses, and during 94.55 h (similar to 4 days) of fermentation, the maximum enzymatic activity was achieved - CMCase 1.11 IU/ml, Avicelase 0.92 IU/ml, and pectinase 3.69 IU/ml. The obtained crude enzyme mixture was further used for enzymatic hydrolysis of non-treated corn stover and bioethanol production. The reducing sugar yield of 3.85 g/l was obtained under optimal conditions (corn stover 6.6% and time of hydrolysis 78.8 h (similar to 3 days and 7 h)). Scanning electron microscopy revealed structural changes in corn stover samples after enzymatic hydrolysis. Under non-optimized conditions, 0.37% of ethanol was produced by waste brewer's yeast. The obtained results show that bacteria belonging to the genus Hymenobacter have a still unexplored enzymatic potential that could be used for sustainable production of biotechnologically value-added products including biofuels. Specifically, for the first time, a soil bacterium, classified within the genus Hymenobacter, was used for cellulases (CMCase and Avicelase) and pectinase production.", journal = "Environmental Technology & Innovation", title = "Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3", volume = "23", doi = "10.1016/j.eti.2021.101627" }
Mihajlovski, K., Pecarski, D., Rajilić-Stojanović, M.,& Dimitrijević-Branković, S.. (2021). Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3. in Environmental Technology & Innovation, 23. https://doi.org/10.1016/j.eti.2021.101627
Mihajlovski K, Pecarski D, Rajilić-Stojanović M, Dimitrijević-Branković S. Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3. in Environmental Technology & Innovation. 2021;23. doi:10.1016/j.eti.2021.101627 .
Mihajlovski, Katarina, Pecarski, Danijela, Rajilić-Stojanović, Mirjana, Dimitrijević-Branković, Suzana, "Valorization of corn stover and molasses for enzyme synthesis, lignocellulosic hydrolysis and bioethanol production by Hymenobacter sp. CKS3" in Environmental Technology & Innovation, 23 (2021), https://doi.org/10.1016/j.eti.2021.101627 . .