Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers
Само за регистроване кориснике
2021
Аутори
Popovic, MinaStojanovic, Marijana
Veličković, Zlate
Kovacevic, Ana
Miljkovic, Radmila
Mirkovic, Nemanja
Marinković, Aleksandar
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, Lactobacillus reuteri B2 was isolated from the feces of C57BL/6 mice and assessed on probiotic activity. L. reuteri B2 was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0 was 64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2 showed maximum diameters against Klebsiela oxytocaJ7 (12.5 +/- 0.71 mm). We further hypothesized if L. reuteri B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy (SEM), FTIR, and particle size distribution. The high survival rate of L.... reuteri B2 at low pH from 2.0 to 4.0 and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices (SGJ).
Кључне речи:
Probiotics / Lactobacills reuteri / Alginate / Starch maleate / MicroencapsulationИзвор:
International Journal of Biological Macromolecules, 2021, 183, 423-434Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200026 (Универзитет у Београду, Институт за хемију, технологију и металургију - ИХТМ) (RS-MESTD-inst-2020-200026)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200177 (Центар за имунолошка истраживања 'Бранислав Јанковић' Торлак, Београд) (RS-MESTD-inst-2020-200177)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
DOI: 10.1016/j.ijbiomac.2021.04.177
ISSN: 0141-8130
PubMed: 33932415
WoS: 000675542000011
Scopus: 2-s2.0-85105110042
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Popovic, Mina AU - Stojanovic, Marijana AU - Veličković, Zlate AU - Kovacevic, Ana AU - Miljkovic, Radmila AU - Mirkovic, Nemanja AU - Marinković, Aleksandar PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4870 AB - In this study, Lactobacillus reuteri B2 was isolated from the feces of C57BL/6 mice and assessed on probiotic activity. L. reuteri B2 was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0 was 64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2 showed maximum diameters against Klebsiela oxytocaJ7 (12.5 +/- 0.71 mm). We further hypothesized if L. reuteri B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy (SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at low pH from 2.0 to 4.0 and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices (SGJ). T2 - International Journal of Biological Macromolecules T1 - Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers EP - 434 SP - 423 VL - 183 DO - 10.1016/j.ijbiomac.2021.04.177 ER -
@article{ author = "Popovic, Mina and Stojanovic, Marijana and Veličković, Zlate and Kovacevic, Ana and Miljkovic, Radmila and Mirkovic, Nemanja and Marinković, Aleksandar", year = "2021", abstract = "In this study, Lactobacillus reuteri B2 was isolated from the feces of C57BL/6 mice and assessed on probiotic activity. L. reuteri B2 was identified by 16S rDNA sequencing, which the cell viability in acidic conditions at pH 2.0 was 64% after 2 h, and in the presents of 0.30% of the bile salts, after 6 h, was 37%. Antimicrobial assay with L. reuteri B2 showed maximum diameters against Klebsiela oxytocaJ7 (12.5 +/- 0.71 mm). We further hypothesized if L. reuteri B2 strain in the free form can survive all conditions in the gastrointestinal tract (GIT) then the utilization of the appropriate biomaterials would ameliorate its stability and viability in GIT. L. reuteri B2 was microencapsulated into sodium alginate-(Na-alg) and different content of Na-alg and sodium maleate (SM) beads. Characterization materials enveloped their thermal characteristics (TGA/DTA analysis) and structure using: scanning electron microscopy (SEM), FTIR, and particle size distribution. The high survival rate of L. reuteri B2 at low pH from 2.0 to 4.0 and in the presence of the bile salts, at concentrations up to 0.30%, was obtained. L. reuteri B2 showed strong antimicrobial activity and the best protection microencapsulated with Na-alg + SM in simulated gastric juices (SGJ).", journal = "International Journal of Biological Macromolecules", title = "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers", pages = "434-423", volume = "183", doi = "10.1016/j.ijbiomac.2021.04.177" }
Popovic, M., Stojanovic, M., Veličković, Z., Kovacevic, A., Miljkovic, R., Mirkovic, N.,& Marinković, A.. (2021). Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules, 183, 423-434. https://doi.org/10.1016/j.ijbiomac.2021.04.177
Popovic M, Stojanovic M, Veličković Z, Kovacevic A, Miljkovic R, Mirkovic N, Marinković A. Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers. in International Journal of Biological Macromolecules. 2021;183:423-434. doi:10.1016/j.ijbiomac.2021.04.177 .
Popovic, Mina, Stojanovic, Marijana, Veličković, Zlate, Kovacevic, Ana, Miljkovic, Radmila, Mirkovic, Nemanja, Marinković, Aleksandar, "Characterization of potential probiotic strain, L. reuteri B2, and its microencapsulation using alginate-based biopolymers" in International Journal of Biological Macromolecules, 183 (2021):423-434, https://doi.org/10.1016/j.ijbiomac.2021.04.177 . .