The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium
Само за регистроване кориснике
2021
Аутори
Knezevic, Magdalena M.Stajkovic-Srbinovic, Olivera S.
Assel, Maibassova
Milić, Marija
Mihajlovski, Katarina
Delic, Dusica, I
Buntić, Aneta V.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and po...ssess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.
Кључне речи:
Bacillus pseudomycoides / PGPR / Fusarium biocontrol / Chromium tolerance / Alfalfa seed germinationИзвор:
Rhizosphere, 2021, 18Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200011 (Институт за земљиште, Београд) (RS-MESTD-inst-2020-200011)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
DOI: 10.1016/j.rhisph.2021.100353
ISSN: 2452-2198
WoS: 000663433400010
Scopus: 2-s2.0-85104071991
Институција/група
Tehnološko-metalurški fakultetTY - JOUR AU - Knezevic, Magdalena M. AU - Stajkovic-Srbinovic, Olivera S. AU - Assel, Maibassova AU - Milić, Marija AU - Mihajlovski, Katarina AU - Delic, Dusica, I AU - Buntić, Aneta V. PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4919 AB - This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide. T2 - Rhizosphere T1 - The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium VL - 18 DO - 10.1016/j.rhisph.2021.100353 ER -
@article{ author = "Knezevic, Magdalena M. and Stajkovic-Srbinovic, Olivera S. and Assel, Maibassova and Milić, Marija and Mihajlovski, Katarina and Delic, Dusica, I and Buntić, Aneta V.", year = "2021", abstract = "This study was conducted to improve alfalfa seed germination under stress conditions, elevated chromium concentrations or Fusarium infection by seed inoculation. The new Bacillus pseudomycoides strain BM1 was selected as the inoculant according to its ability to suppress plants pathogens and tolerate increased chromium concentrations. This rhizobacteria was isolated and identified based on 16S rDNA sequence from the soil of the rhizosphere of Medicago sativa. Additionally, in this study, the ability of strain BM1 to produce phytohormone Indole-3-Acetic Acid, siderophores, Hydrogen cyanide, extracellular lipase, cellulase, pectinase and to solubilize phosphate was examined. Strain BM1 showed in vitro strong inhibition rate of fungal growth of 59.68%, 48.72% and 43.59% for F. graminearum, F. proliferatum and F. oxysporum f. sp. medicaginis, respectively. To the best of our knowledge, this is the first time that Bacillus pseudomycoides has been reported to have antifungal potential and possess biosynthetic genes for fengycin and subtilin. This rhizobacteria can produce Indole-3-Acetic Acid, siderophores, extracellular lipase, cellulose and pectinase. Inhibition percentage of alfalfa seedling growth infected by F. oxysporum f. sp. medicaginis (in vitro) was reduced from 27.87% to 2.46%. The highest increase of root and shoot length of 12.36% and 21.43% was reached by applying BM1 culture inoculum. Seeds inoculated with BM1 showed no inhibition compared to the control in the presence of a chromium concentration of 0.11 mM L-1 and decreasing inhibition when chromium concentration increased in comparison to control samples (1.54-2.30 mM L-1). The new strain BM1 may be a good choice in phytoremediation of contaminated soil as well as a good active agent in biofertilizers or biofungicide.", journal = "Rhizosphere", title = "The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium", volume = "18", doi = "10.1016/j.rhisph.2021.100353" }
Knezevic, M. M., Stajkovic-Srbinovic, O. S., Assel, M., Milić, M., Mihajlovski, K., Delic, D. I.,& Buntić, A. V.. (2021). The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium. in Rhizosphere, 18. https://doi.org/10.1016/j.rhisph.2021.100353
Knezevic MM, Stajkovic-Srbinovic OS, Assel M, Milić M, Mihajlovski K, Delic DI, Buntić AV. The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium. in Rhizosphere. 2021;18. doi:10.1016/j.rhisph.2021.100353 .
Knezevic, Magdalena M., Stajkovic-Srbinovic, Olivera S., Assel, Maibassova, Milić, Marija, Mihajlovski, Katarina, Delic, Dusica, I, Buntić, Aneta V., "The ability of a new strain of Bacillus pseudomycoides to improve the germination of alfalfa seeds in the presence of fungal infection or chromium" in Rhizosphere, 18 (2021), https://doi.org/10.1016/j.rhisph.2021.100353 . .