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Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage
dc.creator | Jovic, Jelena | |
dc.creator | Hao, Jian | |
dc.creator | Mojović, Ljiljana | |
dc.date.accessioned | 2022-03-04T11:27:23Z | |
dc.date.available | 2022-03-04T11:27:23Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/4975 | |
dc.description.abstract | This study provides a detailed analysis of the lignocellulolytic activity of a new isolate Stereum gausapatum F28, a Serbian autochthonous fungi, on beechwood sawdust supplemented with cheap waste, sugar beet molasses still age. Advanced multiple response optimization techniques were applied to improve ligninolytic and reduce hydrolytic activity as a requirement for potential biorefinery use. The applied techniques were supposed to select cultivation conditions that would give manganese peroxidase and laccase activities above 0.84 and 0.12 U g(-1) substrate, respectively, and cellulase and xylanase activities below 1.12 and 1.4 U g(-1) substrate. The optimal cultivation conditions that met the set requirements included molasses stillage concentration of 10 %, substrate moisture content of 53 %, incubation temperature of 23.5 degrees C, and pH 5.2. The research showed that the addition of molasses stillage had a positive effect on enzyme production and that the optimal stillage concentration differed depending on the enzyme type (for laccase it was lt 5 %, manganese peroxidase approximate to 12 %, cellulase approximate to 21 % and xylanase approximate to 16 %), which should be taken into consideration when optimizing the desired process.. | en |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | response surface method | en |
dc.subject | genetic algorithm | en |
dc.subject | fungi | en |
dc.subject | biomass | en |
dc.subject | enzymes | en |
dc.title | Examination and optimization of lignocellulolytic activity of Stereum gausapatum F28 on beechwood sawdust supplemented with molasses stillage | en |
dc.type | article | |
dc.rights.license | BY | |
dc.citation.rank | M23~ | |
dc.identifier.doi | 10.2298/JSC200601075J | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/7457/Examination_and_optimization_pub_2021.pdf | |
dc.identifier.wos | 000720102300001 | |
dc.type.version | publishedVersion |