Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
Authors
Ponjavić, MarijanaMalagurski, Ivana
Lazić, Jelena
Jeremić, Sanja
Pavlović, Vladimir
Prlainović, Nevena
Maksimović, Vesna
Ćosović, Vladan
Atanase, Leonard Ionut
Article (Published version)
Metadata
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The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8 m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The ca...lculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.
Keywords:
prodigiosin / polyhydroxyalkanoate / PHBV / anticancer activity / film / drug delivery / biopolymer crystallinity / biocompatibilitySource:
International Journal of Molecular Sciences, 2023, 24, 1906-Publisher:
- Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- BioECOLogics - Value-added biologics through eco-sustainable routes (RS-ScienceFundRS-Ideje-7730810)
- Project LA/P/0140/202019 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB
- The national funds from FCT—Fundação para a Ciência e a Tecnologia, in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences—UCIBIO
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Ponjavić, Marijana AU - Malagurski, Ivana AU - Lazić, Jelena AU - Jeremić, Sanja AU - Pavlović, Vladimir AU - Prlainović, Nevena AU - Maksimović, Vesna AU - Ćosović, Vladan AU - Atanase, Leonard Ionut PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/5866 AB - The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8 m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential. PB - Switzerland : Multidisciplinary Digital Publishing Institute (MDPI) T2 - International Journal of Molecular Sciences T1 - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin SP - 1906 VL - 24 DO - 10.3390/ijms24031906 ER -
@article{ author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Ćosović, Vladan and Atanase, Leonard Ionut", year = "2023", abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorableproperties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high productioncosts and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with stronganticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. Thesamples were produced in the form of films 115.6–118.8 m in thickness using the solvent castingmethod. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinityand thermal stability) and functionality of the obtained biomaterials were investigated. PGhas acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability andmorphology of the films. All samples with PG had a more organized internal structure and highermelting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymerwas 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively.Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (coloncancer) cells, thus advancing PHBV biomedical application potential.", publisher = "Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)", journal = "International Journal of Molecular Sciences", title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin", pages = "1906", volume = "24", doi = "10.3390/ijms24031906" }
Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Ćosović, V.,& Atanase, L. I.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences Switzerland : Multidisciplinary Digital Publishing Institute (MDPI)., 24, 1906. https://doi.org/10.3390/ijms24031906
Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Ćosović V, Atanase LI. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24:1906. doi:10.3390/ijms24031906 .
Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Ćosović, Vladan, Atanase, Leonard Ionut, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24 (2023):1906, https://doi.org/10.3390/ijms24031906 . .