Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The microbial enzymes are biological catalysts that perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. Microbial enzymes, obtained by different microorganisms are of great importance for application in industrial bioprocesses [1,2]. The use of microbial enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics [3]. Cellulase are the largest group of industrially important enzymes with a potential to convert cellulose into fermentable sugars [4]. Amylases represents a second largest group of industrial enzymes which are used for the degradation of starch to glucose, maltose, dextrins…[5]. Xylanase are important for pentose production, fruit juice clarification…while pectinase degrade pectic substances in the cell wall of higher plants.They are imp...ortant in food-processing industries, mainly for extraction and clarification of fruit juices and wines, extraction of tomato pulp, oil extraction, and tea and chocolate fermentation [1,2]. All these enzymes (cellulase, amylase, xylanase and pectinase) could be used for agro-industrial residues utilization that lead to bioethanol production.
In this study a crude “enzymes cocktail”, that produce Streptomyces fluvissimus CKS7 was used in a hydrolysis process of different lignocellulosic waste materials. The strain CKS7 produced cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase during solid state fermentation (SSF) on agricultural by-product – rye bran. Obtained crude enzymes were used for hydrolysis of different lignocellulosic substrates: corn stalk waste, Equisetum arvense waste, Gentiana lutea waste, cotton fabric and corona treated cotton fabric. Released reducing sugars was measured during 3 days of hydrolysis at 50 °C. The results revealed that the concentration of reducing sugars was increasing during hydrolysis in all tested lignocellulosic substrates. Although the concentration of released reducing sugars are relatively low (~ 1.9 mg/ml) in all tested samples, this is the first study that deals with enzymatic potential of the S. fluvissimus.
Further experiments should be focused on optimization of enzymatic hydrolysis of lignocellulosic substrates.
Кључне речи:
microbial enzymes / Streptomyces fluvissimus CKS7 / lignocellulosic substrte / hydrolysisИзвор:
Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia, 2018Издавач:
- Society of Chemists and Technologists of Macedonia
Финансирање / пројекти:
- Примена биотехнолошких метода у одрживом искоришћењу нус-производа агроиндустрије (RS-MESTD-Technological Development (TD or TR)-31035)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Mihajlovski, Katarina AU - Milić, Marija AU - Marković, Darka AU - Dimitrijević-Branković, Suzana PY - 2018 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6148 AB - The microbial enzymes are biological catalysts that perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. Microbial enzymes, obtained by different microorganisms are of great importance for application in industrial bioprocesses [1,2]. The use of microbial enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics [3]. Cellulase are the largest group of industrially important enzymes with a potential to convert cellulose into fermentable sugars [4]. Amylases represents a second largest group of industrial enzymes which are used for the degradation of starch to glucose, maltose, dextrins…[5]. Xylanase are important for pentose production, fruit juice clarification…while pectinase degrade pectic substances in the cell wall of higher plants.They are important in food-processing industries, mainly for extraction and clarification of fruit juices and wines, extraction of tomato pulp, oil extraction, and tea and chocolate fermentation [1,2]. All these enzymes (cellulase, amylase, xylanase and pectinase) could be used for agro-industrial residues utilization that lead to bioethanol production. In this study a crude “enzymes cocktail”, that produce Streptomyces fluvissimus CKS7 was used in a hydrolysis process of different lignocellulosic waste materials. The strain CKS7 produced cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase during solid state fermentation (SSF) on agricultural by-product – rye bran. Obtained crude enzymes were used for hydrolysis of different lignocellulosic substrates: corn stalk waste, Equisetum arvense waste, Gentiana lutea waste, cotton fabric and corona treated cotton fabric. Released reducing sugars was measured during 3 days of hydrolysis at 50 °C. The results revealed that the concentration of reducing sugars was increasing during hydrolysis in all tested lignocellulosic substrates. Although the concentration of released reducing sugars are relatively low (~ 1.9 mg/ml) in all tested samples, this is the first study that deals with enzymatic potential of the S. fluvissimus. Further experiments should be focused on optimization of enzymatic hydrolysis of lignocellulosic substrates. PB - Society of Chemists and Technologists of Macedonia C3 - Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia T1 - Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process UR - https://hdl.handle.net/21.15107/rcub_technorep_6148 ER -
@conference{ author = "Mihajlovski, Katarina and Milić, Marija and Marković, Darka and Dimitrijević-Branković, Suzana", year = "2018", abstract = "The microbial enzymes are biological catalysts that perform reactions in bio-processes in an economical and environmentally-friendly way as opposed to the use of chemical catalysts. Microbial enzymes, obtained by different microorganisms are of great importance for application in industrial bioprocesses [1,2]. The use of microbial enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics [3]. Cellulase are the largest group of industrially important enzymes with a potential to convert cellulose into fermentable sugars [4]. Amylases represents a second largest group of industrial enzymes which are used for the degradation of starch to glucose, maltose, dextrins…[5]. Xylanase are important for pentose production, fruit juice clarification…while pectinase degrade pectic substances in the cell wall of higher plants.They are important in food-processing industries, mainly for extraction and clarification of fruit juices and wines, extraction of tomato pulp, oil extraction, and tea and chocolate fermentation [1,2]. All these enzymes (cellulase, amylase, xylanase and pectinase) could be used for agro-industrial residues utilization that lead to bioethanol production. In this study a crude “enzymes cocktail”, that produce Streptomyces fluvissimus CKS7 was used in a hydrolysis process of different lignocellulosic waste materials. The strain CKS7 produced cellulase (CMC-ase and Avicelase), amylase, pectinase and xylanase during solid state fermentation (SSF) on agricultural by-product – rye bran. Obtained crude enzymes were used for hydrolysis of different lignocellulosic substrates: corn stalk waste, Equisetum arvense waste, Gentiana lutea waste, cotton fabric and corona treated cotton fabric. Released reducing sugars was measured during 3 days of hydrolysis at 50 °C. The results revealed that the concentration of reducing sugars was increasing during hydrolysis in all tested lignocellulosic substrates. Although the concentration of released reducing sugars are relatively low (~ 1.9 mg/ml) in all tested samples, this is the first study that deals with enzymatic potential of the S. fluvissimus. Further experiments should be focused on optimization of enzymatic hydrolysis of lignocellulosic substrates.", publisher = "Society of Chemists and Technologists of Macedonia", journal = "Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia", title = "Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process", url = "https://hdl.handle.net/21.15107/rcub_technorep_6148" }
Mihajlovski, K., Milić, M., Marković, D.,& Dimitrijević-Branković, S.. (2018). Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process. in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia Society of Chemists and Technologists of Macedonia.. https://hdl.handle.net/21.15107/rcub_technorep_6148
Mihajlovski K, Milić M, Marković D, Dimitrijević-Branković S. Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process. in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia. 2018;. https://hdl.handle.net/21.15107/rcub_technorep_6148 .
Mihajlovski, Katarina, Milić, Marija, Marković, Darka, Dimitrijević-Branković, Suzana, "Possibility of using microbial enzymes produced by Streptomyces fluvissimus CKS7 in hydrolysis process" in Book of abstracts / 25th Congress of the society of chemists and technologists of Macedonia (2018), https://hdl.handle.net/21.15107/rcub_technorep_6148 .