dc.creator | Mihajlovski, Katarina | |
dc.creator | Davidović, Slađana | |
dc.creator | Miljković, Miona | |
dc.creator | Rajilić-Stojanović, Mirjana | |
dc.creator | Dimitrijević-Branković, Suzana | |
dc.date.accessioned | 2023-03-20T07:57:54Z | |
dc.date.available | 2023-03-20T07:57:54Z | |
dc.date.issued | 2013 | |
dc.identifier.isbn | 978-86-7132-053-5 | |
dc.identifier.uri | http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6160 | |
dc.description.abstract | Cellulases are the enzymes which cause hydrolyzis of the polysaccharide cellulose into
smaller sugar units. Bacterial cellulases possess more advantages when compared to the
cellulases from other sources. The gram positive, spore forming bacteria was isolated from
soil. Strain was identified as Paenibacillus chitinolyticus after biochemical and 16S rRNA
sequencing studies. The isolated strain was screened for cellulase activity by using a
Gram’s iodine metod on carboxymethyl cellulose (CMC) agar plate [1]. It has been found
to produce cellulases which could liberate glucose from CMC (CMC-ase) and
microcristalline cellulose Avicel (avicelase). The enzyme activity was measured by
estimating the liberated reducing sugars using 3,5-dinitrosalicylic acid method [2].
A liquid medium containing 0.5% CMC and yeast extract proved to be a suitable carbon
and nitrogen sources for cellulase production by the bacterium. Maximal cellulase
production for CMC-ase 0.085 U/ml and for avicelase 0.17 U/ml was obtained after 48 h of
incubation at 30 °C under aerobic conditions with 5% overnight culture and with addition
of 0.5% CMC, 10 g/l yeast extract, MgSO4 0.5g/l, KCl 0.5g/l, CaCl2 0.02g/l and 1g/l Tween
80.
Paenibacillus sp. strain isolated from soil is available for further studies aiming the
optimization of its cellulase production, facilitating its potential use in industrial
applications. | sr |
dc.language.iso | en | sr |
dc.publisher | Serbian Chemical Society | sr |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31035/RS// | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | 8th International Conference of the Chemical Societies of the South-East European Countries - ICOSECS 8 | sr |
dc.title | Cellulolytic potencial of a strain Paenibacillus sp. isolated from soil | sr |
dc.type | conferenceObject | sr |
dc.rights.license | BY | sr |
dc.citation.spage | 244 | |
dc.identifier.fulltext | http://TechnoRep.tmf.bg.ac.rs/bitstream/id/16572/bitstream_16572.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_technorep_6160 | |
dc.type.version | publishedVersion | sr |