Design of the new particles for controlled release of bioactive peptides
Само за регистроване кориснике
2019
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Bioactive peptides carry antioxidative activity and have the potential to be used as additives
in food formulation. When incorporated, major instability of peptides is imposed within
heterogeneous products, as well as the loss of biological activity in the gastrointestinal
system before absorption into the bloodstream and manifestation of biological activity at the
target site in the body. A good solution as a controlled release system is liposomes – particles
made out of phospholipids, natural molecules and a building block of the cell membrane.
Therefore, the aim of this study was to prepare new systems for the encapsulation and
controlled release of soybean antioxidant peptides.
Liposome preparation was performed by forming a uniform thin film of phospholipon 90-G
and hydrating it with an aqueous solution of soy protein concentrate hydrolyzate prepared by
a two-step enzymatic process. The last step of liposome preparation was ultrasonic treatment.
The size distribution, s...urface charge, degree of encapsulation and stability of multilamellar
liposomes were characterized. A thin film method provided a satisfying percentage of
encapsulation of soybean antioxidant peptides (19%). The ultrasonic homogenizer proved to
be three times better than the ultrasonic bath while reducing the size of the particles. The
antioxidant activity was tested by neultralization of the ABTS • + radical cation method and
by Fe2+ ion chelation method. The test have shown that encapsulation of the peptide activity
was partially retained. In the simulated gastrointestinal system, diffusion experiments have
shown that liposomes slow down the release of antioxidant peptides.
The presented results represent important information for the specific application of
liposomes with encapsulated soybean peptides in functional food products.
Кључне речи:
Soybean peptides / Radical-scavenging activity / Metal-ion chelating activity / Encapsulation efficiency / LiposomesИзвор:
Book of Abstracts / Eighteenth Young Researchers’ Conference Materials Science and Engineering, 2019, 13-Издавач:
- Belgrade : Institute of Technical Sciences of SASA, 2019 (Belgrade : Gama digital centar)
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- EUREKA Project / SOYZYME E! 9936 - Design of novel enzyme-based technologies for structuring and processing of soy proteins
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Paunović, Anđela AU - Knežević-Jugović, Zorica AU - Jovanović, Jelena AU - Pavlović, Neda PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6256 AB - Bioactive peptides carry antioxidative activity and have the potential to be used as additives in food formulation. When incorporated, major instability of peptides is imposed within heterogeneous products, as well as the loss of biological activity in the gastrointestinal system before absorption into the bloodstream and manifestation of biological activity at the target site in the body. A good solution as a controlled release system is liposomes – particles made out of phospholipids, natural molecules and a building block of the cell membrane. Therefore, the aim of this study was to prepare new systems for the encapsulation and controlled release of soybean antioxidant peptides. Liposome preparation was performed by forming a uniform thin film of phospholipon 90-G and hydrating it with an aqueous solution of soy protein concentrate hydrolyzate prepared by a two-step enzymatic process. The last step of liposome preparation was ultrasonic treatment. The size distribution, surface charge, degree of encapsulation and stability of multilamellar liposomes were characterized. A thin film method provided a satisfying percentage of encapsulation of soybean antioxidant peptides (19%). The ultrasonic homogenizer proved to be three times better than the ultrasonic bath while reducing the size of the particles. The antioxidant activity was tested by neultralization of the ABTS • + radical cation method and by Fe2+ ion chelation method. The test have shown that encapsulation of the peptide activity was partially retained. In the simulated gastrointestinal system, diffusion experiments have shown that liposomes slow down the release of antioxidant peptides. The presented results represent important information for the specific application of liposomes with encapsulated soybean peptides in functional food products. PB - Belgrade : Institute of Technical Sciences of SASA, 2019 (Belgrade : Gama digital centar) C3 - Book of Abstracts / Eighteenth Young Researchers’ Conference Materials Science and Engineering T1 - Design of the new particles for controlled release of bioactive peptides SP - 13 UR - https://hdl.handle.net/21.15107/rcub_technorep_6256 ER -
@conference{ author = "Paunović, Anđela and Knežević-Jugović, Zorica and Jovanović, Jelena and Pavlović, Neda", year = "2019", abstract = "Bioactive peptides carry antioxidative activity and have the potential to be used as additives in food formulation. When incorporated, major instability of peptides is imposed within heterogeneous products, as well as the loss of biological activity in the gastrointestinal system before absorption into the bloodstream and manifestation of biological activity at the target site in the body. A good solution as a controlled release system is liposomes – particles made out of phospholipids, natural molecules and a building block of the cell membrane. Therefore, the aim of this study was to prepare new systems for the encapsulation and controlled release of soybean antioxidant peptides. Liposome preparation was performed by forming a uniform thin film of phospholipon 90-G and hydrating it with an aqueous solution of soy protein concentrate hydrolyzate prepared by a two-step enzymatic process. The last step of liposome preparation was ultrasonic treatment. The size distribution, surface charge, degree of encapsulation and stability of multilamellar liposomes were characterized. A thin film method provided a satisfying percentage of encapsulation of soybean antioxidant peptides (19%). The ultrasonic homogenizer proved to be three times better than the ultrasonic bath while reducing the size of the particles. The antioxidant activity was tested by neultralization of the ABTS • + radical cation method and by Fe2+ ion chelation method. The test have shown that encapsulation of the peptide activity was partially retained. In the simulated gastrointestinal system, diffusion experiments have shown that liposomes slow down the release of antioxidant peptides. The presented results represent important information for the specific application of liposomes with encapsulated soybean peptides in functional food products.", publisher = "Belgrade : Institute of Technical Sciences of SASA, 2019 (Belgrade : Gama digital centar)", journal = "Book of Abstracts / Eighteenth Young Researchers’ Conference Materials Science and Engineering", title = "Design of the new particles for controlled release of bioactive peptides", pages = "13", url = "https://hdl.handle.net/21.15107/rcub_technorep_6256" }
Paunović, A., Knežević-Jugović, Z., Jovanović, J.,& Pavlović, N.. (2019). Design of the new particles for controlled release of bioactive peptides. in Book of Abstracts / Eighteenth Young Researchers’ Conference Materials Science and Engineering Belgrade : Institute of Technical Sciences of SASA, 2019 (Belgrade : Gama digital centar)., 13. https://hdl.handle.net/21.15107/rcub_technorep_6256
Paunović A, Knežević-Jugović Z, Jovanović J, Pavlović N. Design of the new particles for controlled release of bioactive peptides. in Book of Abstracts / Eighteenth Young Researchers’ Conference Materials Science and Engineering. 2019;:13. https://hdl.handle.net/21.15107/rcub_technorep_6256 .
Paunović, Anđela, Knežević-Jugović, Zorica, Jovanović, Jelena, Pavlović, Neda, "Design of the new particles for controlled release of bioactive peptides" in Book of Abstracts / Eighteenth Young Researchers’ Conference Materials Science and Engineering (2019):13, https://hdl.handle.net/21.15107/rcub_technorep_6256 .