The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts
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2021
Authors
Jovanović, Aleksandra A.Petrović, Predrag
Đorđević, Verica
Zdunić, Gordana
Šavikin, Katarina
Nedović, Viktor
Bugarski, Branko
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The aim of the present study was the investigation of the impact of different extraction
conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and
extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts
were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator
shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of
80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1,
20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant
potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of
variance and Duncan’s post-hoc test); the difference was considered statistically significant
...at p<0.05. According to the results, higher degree of herbal drug disintegration resulted
with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted
extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the
increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential
regardless of the type of extraction method. Furthermore, in comparison to aqueous wild
thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the
case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant
activity of the extracts obtained by different extraction procedures, while in neutralization
of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly
higher antioxidant potential. Due to the antioxidant properties shown in the present study,
wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and
cosmetic industries.
Keywords:
antioxidant activity / extraction / polyphenols / wild thymeSource:
14th Congress of Nutrition, 2021, -76Publisher:
- Serbian Nutrition Society
Institution/Community
Inovacioni centarTY - CONF AU - Jovanović, Aleksandra A. AU - Petrović, Predrag AU - Đorđević, Verica AU - Zdunić, Gordana AU - Šavikin, Katarina AU - Nedović, Viktor AU - Bugarski, Branko PY - 2021 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6441 AB - The aim of the present study was the investigation of the impact of different extraction conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of 80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1, 20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of variance and Duncan’s post-hoc test); the difference was considered statistically significant at p<0.05. According to the results, higher degree of herbal drug disintegration resulted with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential regardless of the type of extraction method. Furthermore, in comparison to aqueous wild thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant activity of the extracts obtained by different extraction procedures, while in neutralization of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly higher antioxidant potential. Due to the antioxidant properties shown in the present study, wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and cosmetic industries. PB - Serbian Nutrition Society C3 - 14th Congress of Nutrition T1 - The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts EP - 76 UR - https://hdl.handle.net/21.15107/rcub_technorep_6441 ER -
@conference{ author = "Jovanović, Aleksandra A. and Petrović, Predrag and Đorđević, Verica and Zdunić, Gordana and Šavikin, Katarina and Nedović, Viktor and Bugarski, Branko", year = "2021", abstract = "The aim of the present study was the investigation of the impact of different extraction conditions (particle size of plant material, solvent-to-solid ratio and solvent type) and extraction techniques (maceration, heat- and ultrasound-assisted extractions) on the antioxidant activity of wild thyme (Thymus serpyllum L.) extracts. Liquid wild thyme extracts were obtained in maceration (in shaker at 25°C), in heat-assisted extraction (in incubator shaker at 80°C) and in ultrasound-assisted extraction (by ultrasound probe at amplitude of 80%) using three particle sizes (0.3, 0.7, and 1.5 mm), three solvent-to-solid ratios (10:1, 20:1, and 30:1), and three ethanol concentrations (0, 30 and 50 % ethanol). Antioxidant potential of the obtained extracts was determined using ABTS and DPPH assays. The statistical analysis was performed by using statistical software, STATISTICA 7.0 (analysis of variance and Duncan’s post-hoc test); the difference was considered statistically significant at p<0.05. According to the results, higher degree of herbal drug disintegration resulted with better antioxidant activity, but this effect was statistically significant for heat- and ultrasound-assisted extractions according to ABTS assay, and for maceration and heat-assisted extraction according to DPPH assay. According to the influence of solvent-to-solid ratio, the increase in ratio from 10:1 to 30:1 resulted in the extracts with higher antioxidant potential regardless of the type of extraction method. Furthermore, in comparison to aqueous wild thyme extracts the antioxidant activity of ethanol extracts was higher, particularly in the case of 50 % ethanol extracts. In ABTS assay, there were no differences between antioxidant activity of the extracts obtained by different extraction procedures, while in neutralization of DPPH radicals, the extracts obtained using ultrasonic probe had statistically significantly higher antioxidant potential. Due to the antioxidant properties shown in the present study, wild thyme ethanol extract may be used in the formulations in food, pharmaceutical, and cosmetic industries.", publisher = "Serbian Nutrition Society", journal = "14th Congress of Nutrition", title = "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts", pages = "76", url = "https://hdl.handle.net/21.15107/rcub_technorep_6441" }
Jovanović, A. A., Petrović, P., Đorđević, V., Zdunić, G., Šavikin, K., Nedović, V.,& Bugarski, B.. (2021). The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition Serbian Nutrition Society.. https://hdl.handle.net/21.15107/rcub_technorep_6441
Jovanović AA, Petrović P, Đorđević V, Zdunić G, Šavikin K, Nedović V, Bugarski B. The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts. in 14th Congress of Nutrition. 2021;:null-76. https://hdl.handle.net/21.15107/rcub_technorep_6441 .
Jovanović, Aleksandra A., Petrović, Predrag, Đorđević, Verica, Zdunić, Gordana, Šavikin, Katarina, Nedović, Viktor, Bugarski, Branko, "The influence of different extraction conditions and extraction techniques on antioxidant activity of wild thyme extracts" in 14th Congress of Nutrition (2021), https://hdl.handle.net/21.15107/rcub_technorep_6441 .