Development of Lipid-β-Sitosterol Small Unilamelar Liposomes as Vehicles for Gentisic Acid
Само за регистроване кориснике
2019
Аутори
Jovanović, Aleksandra A.Balanč, Bojana D.
Đorđević, Verica B.
Ota, Ajda
Bugarski, Branko M.
Nedović, Viktor A.
Poklar Ulrih, Nataša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Liposomes, as biodegradable, non-toxic and non-immunogenic drug delivery systems, can
provide controlled release of active molecules and their protection from degradation. Therefore,
the aim of the present study was encapsulation of gentisic acid (antioxidant polyphenols acid)
into liposomes containing phospholipids (Lipoid) and various amounts of β-sitosterol. Liposomal
bilayer containing β-sitosterol was more fluid and flexible, in comparison to pure Lipoid
liposomes. Gentisic acid has caused the rearranging of the acid chains of phospholipids bilayer
in the 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMADPH) environment. Liposomal vesicles containing Lipoid and β-sitosterol have smaller diameter
compared to pure Lipoid, whereas there is no statistically significant difference in polydispersity
index (PDI). Additionally, higher concentration of β-sitosterol has induced the increase of
absolute zeta potential values and consequently higher stab...ility of liposomal suspension. The
addition of various amounts of gentisic acid did not influence the changes of particle size and
PDI values, while in Lipoid liposomes gentisic acid has induced significant and gradual increase
of zeta potential. Lipid peroxidation was higher in Lipoid-β-sitosterol system, particularly in
Lipoid 50%-β-sitosterol 50% sample, whereas gentisic acid has reduced rate of membrane lipid
peroxidation. In comparison to pure Lipoid, Lipoid-β-sitosterol system has shown better
membrane permeability, smaller particle size, higher zeta potential and stability, which made
Lipoid-β-sitosterol liposomes suitable delivery system for gentisic acid.
Кључне речи:
β sitosterol / encapsulation / gentisic acid / liposomes / phospholipidsИзвор:
VI International Congress: „Engineering, Environment and Materials in Processing Industry“, 2019, 156-166Издавач:
- Faculty of Technology, Zvornik
Финансирање / пројекти:
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
Институција/група
Inovacioni centarTY - CONF AU - Jovanović, Aleksandra A. AU - Balanč, Bojana D. AU - Đorđević, Verica B. AU - Ota, Ajda AU - Bugarski, Branko M. AU - Nedović, Viktor A. AU - Poklar Ulrih, Nataša PY - 2019 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6456 AB - Liposomes, as biodegradable, non-toxic and non-immunogenic drug delivery systems, can provide controlled release of active molecules and their protection from degradation. Therefore, the aim of the present study was encapsulation of gentisic acid (antioxidant polyphenols acid) into liposomes containing phospholipids (Lipoid) and various amounts of β-sitosterol. Liposomal bilayer containing β-sitosterol was more fluid and flexible, in comparison to pure Lipoid liposomes. Gentisic acid has caused the rearranging of the acid chains of phospholipids bilayer in the 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMADPH) environment. Liposomal vesicles containing Lipoid and β-sitosterol have smaller diameter compared to pure Lipoid, whereas there is no statistically significant difference in polydispersity index (PDI). Additionally, higher concentration of β-sitosterol has induced the increase of absolute zeta potential values and consequently higher stability of liposomal suspension. The addition of various amounts of gentisic acid did not influence the changes of particle size and PDI values, while in Lipoid liposomes gentisic acid has induced significant and gradual increase of zeta potential. Lipid peroxidation was higher in Lipoid-β-sitosterol system, particularly in Lipoid 50%-β-sitosterol 50% sample, whereas gentisic acid has reduced rate of membrane lipid peroxidation. In comparison to pure Lipoid, Lipoid-β-sitosterol system has shown better membrane permeability, smaller particle size, higher zeta potential and stability, which made Lipoid-β-sitosterol liposomes suitable delivery system for gentisic acid. PB - Faculty of Technology, Zvornik C3 - VI International Congress: „Engineering, Environment and Materials in Processing Industry“ T1 - Development of Lipid-β-Sitosterol Small Unilamelar Liposomes as Vehicles for Gentisic Acid EP - 166 SP - 156 DO - 10.7251/EEMEN1901156J ER -
@conference{ author = "Jovanović, Aleksandra A. and Balanč, Bojana D. and Đorđević, Verica B. and Ota, Ajda and Bugarski, Branko M. and Nedović, Viktor A. and Poklar Ulrih, Nataša", year = "2019", abstract = "Liposomes, as biodegradable, non-toxic and non-immunogenic drug delivery systems, can provide controlled release of active molecules and their protection from degradation. Therefore, the aim of the present study was encapsulation of gentisic acid (antioxidant polyphenols acid) into liposomes containing phospholipids (Lipoid) and various amounts of β-sitosterol. Liposomal bilayer containing β-sitosterol was more fluid and flexible, in comparison to pure Lipoid liposomes. Gentisic acid has caused the rearranging of the acid chains of phospholipids bilayer in the 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMADPH) environment. Liposomal vesicles containing Lipoid and β-sitosterol have smaller diameter compared to pure Lipoid, whereas there is no statistically significant difference in polydispersity index (PDI). Additionally, higher concentration of β-sitosterol has induced the increase of absolute zeta potential values and consequently higher stability of liposomal suspension. The addition of various amounts of gentisic acid did not influence the changes of particle size and PDI values, while in Lipoid liposomes gentisic acid has induced significant and gradual increase of zeta potential. Lipid peroxidation was higher in Lipoid-β-sitosterol system, particularly in Lipoid 50%-β-sitosterol 50% sample, whereas gentisic acid has reduced rate of membrane lipid peroxidation. In comparison to pure Lipoid, Lipoid-β-sitosterol system has shown better membrane permeability, smaller particle size, higher zeta potential and stability, which made Lipoid-β-sitosterol liposomes suitable delivery system for gentisic acid.", publisher = "Faculty of Technology, Zvornik", journal = "VI International Congress: „Engineering, Environment and Materials in Processing Industry“", title = "Development of Lipid-β-Sitosterol Small Unilamelar Liposomes as Vehicles for Gentisic Acid", pages = "166-156", doi = "10.7251/EEMEN1901156J" }
Jovanović, A. A., Balanč, B. D., Đorđević, V. B., Ota, A., Bugarski, B. M., Nedović, V. A.,& Poklar Ulrih, N.. (2019). Development of Lipid-β-Sitosterol Small Unilamelar Liposomes as Vehicles for Gentisic Acid. in VI International Congress: „Engineering, Environment and Materials in Processing Industry“ Faculty of Technology, Zvornik., 156-166. https://doi.org/10.7251/EEMEN1901156J
Jovanović AA, Balanč BD, Đorđević VB, Ota A, Bugarski BM, Nedović VA, Poklar Ulrih N. Development of Lipid-β-Sitosterol Small Unilamelar Liposomes as Vehicles for Gentisic Acid. in VI International Congress: „Engineering, Environment and Materials in Processing Industry“. 2019;:156-166. doi:10.7251/EEMEN1901156J .
Jovanović, Aleksandra A., Balanč, Bojana D., Đorđević, Verica B., Ota, Ajda, Bugarski, Branko M., Nedović, Viktor A., Poklar Ulrih, Nataša, "Development of Lipid-β-Sitosterol Small Unilamelar Liposomes as Vehicles for Gentisic Acid" in VI International Congress: „Engineering, Environment and Materials in Processing Industry“ (2019):156-166, https://doi.org/10.7251/EEMEN1901156J . .