Effective approach for obtaining high-purity prebiotics using membrane separation technology
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2023
Authors
Veljković, MilicaSimović, Milica
Banjanac, Katarina
Milivojević, Ana
Ćorović, Marija
Vukoičić, Ana
Bezbradica, Dejan
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With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity... of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.
Keywords:
prebiotics / fructo-oligosaccharides / nanofiltration / enzymatic synthesisSource:
Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023, 2023, 25-Publisher:
- Belgrade : University, Faculty of Geography
Funding / projects:
- PrIntPrEnzy - Prebiotics for functional food and bioactive cosmetics produced in intensified enzymatic processes (RS-ScienceFundRS-Ideje-7750109)
- Horizon Europe 2021-2027 research and innovation programme under grant agreement ID 101060130
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Inovacioni centarTY - CONF AU - Veljković, Milica AU - Simović, Milica AU - Banjanac, Katarina AU - Milivojević, Ana AU - Ćorović, Marija AU - Vukoičić, Ana AU - Bezbradica, Dejan PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6843 AB - With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained. PB - Belgrade : University, Faculty of Geography C3 - Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 T1 - Effective approach for obtaining high-purity prebiotics using membrane separation technology SP - 25 UR - https://hdl.handle.net/21.15107/rcub_technorep_6843 ER -
@conference{ author = "Veljković, Milica and Simović, Milica and Banjanac, Katarina and Milivojević, Ana and Ćorović, Marija and Vukoičić, Ana and Bezbradica, Dejan", year = "2023", abstract = "With the increasing interest in functional products, prebiotics that selectively stimulate growth and activity of good bacteria in human gut, gained attention from both general and scientific community. The range of food products containing prebiotic oligosaccharides is rapidly expanding, causing their intensified production. Currently, there are a few recognized prebiotics, amongst which, fructo-oligosaccharides (FOS) proved to be particularly interesting owing to the confirmed positive effect on human wellbeing (restoring microbiota balance, improving immunity and lipid metabolism, enhancing vitamin production and calcium absorption) and exceptional physicochemical qualities. Namely, good taste quality, low sweetness, and low caloric value make them highly applicable supplement in food industry. Fructo-oligosaccharides are scarcely present in natural sources and nowadays are mainly produced by enzymatic conversion of sucrose and sucrose-rich substrates. Bearing in mind the complexity of synthesis process, resulting reaction mixture besides FOS (mixture of tri-, tetra- and pentasaccharides) contains significant portion of sucrose and monosaccharides. Within this study, efficient method for obtaining high-purity product upon sucrose conversion using fructosyltransferase (Pectinex® Ultra SP-L) was developed through detailed optimization of membrane separation processes. Under determined optimal conditions for nanofiltration (temperature of 35 °C, concentration of initial sugars of 3% and flow rate of 25 mL/min), undesired components (monosaccharides and partially sucrose) were removed from the initial mixture (58% FOS in total carbohydrates) and 1.48-fold increase the proportion of targeted bioactive components was achieved. Thus, product of high purity (86% FOS in total carbohydrates) was obtained with remarkable recovery yields of 90%. The prebiotic activity of the obtained product was confirmed in in vitro tests with several microbial cultures comprising representatives of beneficial (genera Lactobacillus and Saccharomyces) and pathogenic (Escherichia coli) gut microbiota. Having said that, it can be concluded that high-purity prebiotic product with great potential for application in different value-added food industry products was obtained.", publisher = "Belgrade : University, Faculty of Geography", journal = "Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023", title = "Effective approach for obtaining high-purity prebiotics using membrane separation technology", pages = "25", url = "https://hdl.handle.net/21.15107/rcub_technorep_6843" }
Veljković, M., Simović, M., Banjanac, K., Milivojević, A., Ćorović, M., Vukoičić, A.,& Bezbradica, D.. (2023). Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 Belgrade : University, Faculty of Geography., 25. https://hdl.handle.net/21.15107/rcub_technorep_6843
Veljković M, Simović M, Banjanac K, Milivojević A, Ćorović M, Vukoičić A, Bezbradica D. Effective approach for obtaining high-purity prebiotics using membrane separation technology. in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023. 2023;:25. https://hdl.handle.net/21.15107/rcub_technorep_6843 .
Veljković, Milica, Simović, Milica, Banjanac, Katarina, Milivojević, Ana, Ćorović, Marija, Vukoičić, Ana, Bezbradica, Dejan, "Effective approach for obtaining high-purity prebiotics using membrane separation technology" in Book of Abstracts / International Scientific Conference Green Agenda for Western Balkans, Belgrade, June 20-22, 2023 (2023):25, https://hdl.handle.net/21.15107/rcub_technorep_6843 .