By-products from the processing of herbs as sources of antioxidants
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2023
Authors
Mladenović, MihailoGrbić, Jovana
Moskovljević, Mia
Bogdanović, Mihajlo
Mladenović, Dragana
Lukić, Ivana
Đukić-Vuković, Aleksandra
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During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and
basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the ...obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu
method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.
Keywords:
hoary willowherb / sage / basil / waste / circular economySource:
Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade, 2023, 20-Publisher:
- Belgrade : University, Faculty of Technology and Metallurgy
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200287 (Innovation Center of the Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200287)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200135 (University of Belgrade, Faculty of Technology and Metallurgy) (RS-MESTD-inst-2020-200135)
- Alliance of International Science Organizations, project SparkGREEN (ANSO-CR-PP 2022-08)
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Institution/Community
Tehnološko-metalurški fakultetTY - CONF AU - Mladenović, Mihailo AU - Grbić, Jovana AU - Moskovljević, Mia AU - Bogdanović, Mihajlo AU - Mladenović, Dragana AU - Lukić, Ivana AU - Đukić-Vuković, Aleksandra PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6944 AB - During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations. PB - Belgrade : University, Faculty of Technology and Metallurgy C3 - Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade T1 - By-products from the processing of herbs as sources of antioxidants SP - 20 UR - https://hdl.handle.net/21.15107/rcub_technorep_6944 ER -
@conference{ author = "Mladenović, Mihailo and Grbić, Jovana and Moskovljević, Mia and Bogdanović, Mihajlo and Mladenović, Dragana and Lukić, Ivana and Đukić-Vuković, Aleksandra", year = "2023", abstract = "During the processing of herbs, a significant amount of chopped biomass by-products is left behind, often discarded and underutilized. Since they are important sources of different groups of bioactives like polyphenolics, essential oils or terpenes and structural compounds like lignocellulose, they could be exploited in fermentation by different microorganisms. However, fractionation and extractions could significantly increase the effectiveness and productivity of the valorization process. We examined the possibility of extracting antioxidants from by-products of hoary willowherb (Epilobium spp.), sage (Salvia spp.), and basil (Ocimum spp.), as medicinal herbs with significant pharmaceutical and market value. Extracts of hoary willowherb, sage and basil residues were obtained by a conventional, Soxhlet extraction for polar compounds and supercritical carbon(IV)-oxide extraction as an unconventional extraction method for non-polar compounds. We measured the antioxidant content in the obtained extracts by the ABTS and DPPH spectrophotometric methods. In addition, we measured the total polyphenol content of the extracts by the Folin-Coicalteu method. According to the ABTS method, the antioxidant activity in the sage extracts obtained from Soxhlet extraction is 2.2 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity in the basil extracts obtained from Soxhlet extraction is 9.1 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction, according to the DPPH method. The total polyphenol content in the hoary willowherb extracts obtained from Soxhlet extraction is 4.6 times greater than in the extracts obtained from supercritical carbon(IV)-oxide extraction. The antioxidant activity is greater in the extracts obtained by Soxhlet extraction than in the extracts obtained with supercritical carbon(IV)-oxide extraction. Because we examined residues from the tea processing industry, the content of easily volatile non-polar components is expected to be lower. The results confirm that valorization of these residues should be focused on the polar components and their microbial biotransformations.", publisher = "Belgrade : University, Faculty of Technology and Metallurgy", journal = "Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade", title = "By-products from the processing of herbs as sources of antioxidants", pages = "20", url = "https://hdl.handle.net/21.15107/rcub_technorep_6944" }
Mladenović, M., Grbić, J., Moskovljević, M., Bogdanović, M., Mladenović, D., Lukić, I.,& Đukić-Vuković, A.. (2023). By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade Belgrade : University, Faculty of Technology and Metallurgy., 20. https://hdl.handle.net/21.15107/rcub_technorep_6944
Mladenović M, Grbić J, Moskovljević M, Bogdanović M, Mladenović D, Lukić I, Đukić-Vuković A. By-products from the processing of herbs as sources of antioxidants. in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade. 2023;:20. https://hdl.handle.net/21.15107/rcub_technorep_6944 .
Mladenović, Mihailo, Grbić, Jovana, Moskovljević, Mia, Bogdanović, Mihajlo, Mladenović, Dragana, Lukić, Ivana, Đukić-Vuković, Aleksandra, "By-products from the processing of herbs as sources of antioxidants" in Book of Abstracts / International Conference on Biochemical Engineering and Biotechnology for Young Scientists, 7-8 December, 2023, Belgrade (2023):20, https://hdl.handle.net/21.15107/rcub_technorep_6944 .