Advancement of biocompatibility and mechanical surface characteristics of the Ti-13Nb-13Zr alloy using electrochemical anodization
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The coarse-grained (CG) and ultrafine-grained (UFG) Ti-13Nb-13Zr alloy (TNZ) were used as tested materials in this
study. UFG alloy was obtained using a high-pressure torsion process (HPT) at room temperature, with 5 rotations, and
with 4.1 GPa pressure. After that, the surface was modified using electrochemical anodization in the 1M H3PO4 + NaF
electrolyte, for 60 and 90 minutes.
Scanning electron microscopy (SEM) was used to characterize the morphology of the modified surface after
electrochemical anodization. In order to determine whether the electrochemical anodization leads to obtaining the
surface modulus of elasticity values close to the values characteristic of the human bones, a nanoindentation test was
done. The nanoindentation test was performed on the device called nanoindenter G200, Agilent Technologies, where
a diamond tip of the Berkovich type shaped as a three-sided pyramid was used as an indenter. Control of
the nanoindentation test was done by nanoindentation... depth, where for non-anodized materials a depth of 2000 nm was
used, while for anodized materials a minimum value of 10% of the thickness of the formed nanostructured oxide layer
was used. Biocompatibility of the tested titanium alloy was estimated by the tetrazolium salt colorimetric test
(MTT test) using mouse fibroblasts (L-929) and human lung fibroblasts (MRC-5). The cell adhesion on the alloy
surface was analyzed using SEM.
Electrochemical anodization has led to the formation of the nanostructured oxide layer on the titanium alloy surface.
The obtained results show the existence of the influence of anodizing time on the nanostructured oxide layer
morphology. Characterization of the titanium alloy surface using nanoindentation showed a decrease in the values
of modulus of elasticity for an alloy with a nanostructured oxide layer, which is close to the values of bone tissue in
the human body. The results of the MTT test showed that the titanium alloys before and after electrochemical
anodization were not cytotoxic. After electrochemical anodization, a uniform nanostructured surface, with nanotubes,
was formed which led to better cell viability and adhesion of L-929 and MRC-5 cells in contact with the test alloys than
of cells in contact with the control material.
Кључне речи:
High-pressure torsion / Ultrafine-grained Ti-13Nb-13Zr alloy / Electrochemical anodization / Biocompatibility / Surface modulus of elasticityИзвор:
Book of Abstracts / 5th Metallurgical & Materials Engineering Congress of SouthEast Europe MME SEE Congress 2023, Trebinje, Bosnia and Herzegovina 7-10th June 2023, 2023, 56-Издавач:
- Belgrade : Association of Metallurgical Engineers of Serbia (AMES)
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
Институција/група
Tehnološko-metalurški fakultetTY - CONF AU - Mihajlović, Dragana AU - Rakin, Marko AU - Veljović, Đorđe AU - Međo, Bojan AU - Đokić, Veljko PY - 2023 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/7011 AB - The coarse-grained (CG) and ultrafine-grained (UFG) Ti-13Nb-13Zr alloy (TNZ) were used as tested materials in this study. UFG alloy was obtained using a high-pressure torsion process (HPT) at room temperature, with 5 rotations, and with 4.1 GPa pressure. After that, the surface was modified using electrochemical anodization in the 1M H3PO4 + NaF electrolyte, for 60 and 90 minutes. Scanning electron microscopy (SEM) was used to characterize the morphology of the modified surface after electrochemical anodization. In order to determine whether the electrochemical anodization leads to obtaining the surface modulus of elasticity values close to the values characteristic of the human bones, a nanoindentation test was done. The nanoindentation test was performed on the device called nanoindenter G200, Agilent Technologies, where a diamond tip of the Berkovich type shaped as a three-sided pyramid was used as an indenter. Control of the nanoindentation test was done by nanoindentation depth, where for non-anodized materials a depth of 2000 nm was used, while for anodized materials a minimum value of 10% of the thickness of the formed nanostructured oxide layer was used. Biocompatibility of the tested titanium alloy was estimated by the tetrazolium salt colorimetric test (MTT test) using mouse fibroblasts (L-929) and human lung fibroblasts (MRC-5). The cell adhesion on the alloy surface was analyzed using SEM. Electrochemical anodization has led to the formation of the nanostructured oxide layer on the titanium alloy surface. The obtained results show the existence of the influence of anodizing time on the nanostructured oxide layer morphology. Characterization of the titanium alloy surface using nanoindentation showed a decrease in the values of modulus of elasticity for an alloy with a nanostructured oxide layer, which is close to the values of bone tissue in the human body. The results of the MTT test showed that the titanium alloys before and after electrochemical anodization were not cytotoxic. After electrochemical anodization, a uniform nanostructured surface, with nanotubes, was formed which led to better cell viability and adhesion of L-929 and MRC-5 cells in contact with the test alloys than of cells in contact with the control material. PB - Belgrade : Association of Metallurgical Engineers of Serbia (AMES) C3 - Book of Abstracts / 5th Metallurgical & Materials Engineering Congress of SouthEast Europe MME SEE Congress 2023, Trebinje, Bosnia and Herzegovina 7-10th June 2023 T1 - Advancement of biocompatibility and mechanical surface characteristics of the Ti-13Nb-13Zr alloy using electrochemical anodization SP - 56 UR - https://hdl.handle.net/21.15107/rcub_technorep_7011 ER -
@conference{ author = "Mihajlović, Dragana and Rakin, Marko and Veljović, Đorđe and Međo, Bojan and Đokić, Veljko", year = "2023", abstract = "The coarse-grained (CG) and ultrafine-grained (UFG) Ti-13Nb-13Zr alloy (TNZ) were used as tested materials in this study. UFG alloy was obtained using a high-pressure torsion process (HPT) at room temperature, with 5 rotations, and with 4.1 GPa pressure. After that, the surface was modified using electrochemical anodization in the 1M H3PO4 + NaF electrolyte, for 60 and 90 minutes. Scanning electron microscopy (SEM) was used to characterize the morphology of the modified surface after electrochemical anodization. In order to determine whether the electrochemical anodization leads to obtaining the surface modulus of elasticity values close to the values characteristic of the human bones, a nanoindentation test was done. The nanoindentation test was performed on the device called nanoindenter G200, Agilent Technologies, where a diamond tip of the Berkovich type shaped as a three-sided pyramid was used as an indenter. Control of the nanoindentation test was done by nanoindentation depth, where for non-anodized materials a depth of 2000 nm was used, while for anodized materials a minimum value of 10% of the thickness of the formed nanostructured oxide layer was used. Biocompatibility of the tested titanium alloy was estimated by the tetrazolium salt colorimetric test (MTT test) using mouse fibroblasts (L-929) and human lung fibroblasts (MRC-5). The cell adhesion on the alloy surface was analyzed using SEM. Electrochemical anodization has led to the formation of the nanostructured oxide layer on the titanium alloy surface. The obtained results show the existence of the influence of anodizing time on the nanostructured oxide layer morphology. Characterization of the titanium alloy surface using nanoindentation showed a decrease in the values of modulus of elasticity for an alloy with a nanostructured oxide layer, which is close to the values of bone tissue in the human body. The results of the MTT test showed that the titanium alloys before and after electrochemical anodization were not cytotoxic. After electrochemical anodization, a uniform nanostructured surface, with nanotubes, was formed which led to better cell viability and adhesion of L-929 and MRC-5 cells in contact with the test alloys than of cells in contact with the control material.", publisher = "Belgrade : Association of Metallurgical Engineers of Serbia (AMES)", journal = "Book of Abstracts / 5th Metallurgical & Materials Engineering Congress of SouthEast Europe MME SEE Congress 2023, Trebinje, Bosnia and Herzegovina 7-10th June 2023", title = "Advancement of biocompatibility and mechanical surface characteristics of the Ti-13Nb-13Zr alloy using electrochemical anodization", pages = "56", url = "https://hdl.handle.net/21.15107/rcub_technorep_7011" }
Mihajlović, D., Rakin, M., Veljović, Đ., Međo, B.,& Đokić, V.. (2023). Advancement of biocompatibility and mechanical surface characteristics of the Ti-13Nb-13Zr alloy using electrochemical anodization. in Book of Abstracts / 5th Metallurgical & Materials Engineering Congress of SouthEast Europe MME SEE Congress 2023, Trebinje, Bosnia and Herzegovina 7-10th June 2023 Belgrade : Association of Metallurgical Engineers of Serbia (AMES)., 56. https://hdl.handle.net/21.15107/rcub_technorep_7011
Mihajlović D, Rakin M, Veljović Đ, Međo B, Đokić V. Advancement of biocompatibility and mechanical surface characteristics of the Ti-13Nb-13Zr alloy using electrochemical anodization. in Book of Abstracts / 5th Metallurgical & Materials Engineering Congress of SouthEast Europe MME SEE Congress 2023, Trebinje, Bosnia and Herzegovina 7-10th June 2023. 2023;:56. https://hdl.handle.net/21.15107/rcub_technorep_7011 .
Mihajlović, Dragana, Rakin, Marko, Veljović, Đorđe, Međo, Bojan, Đokić, Veljko, "Advancement of biocompatibility and mechanical surface characteristics of the Ti-13Nb-13Zr alloy using electrochemical anodization" in Book of Abstracts / 5th Metallurgical & Materials Engineering Congress of SouthEast Europe MME SEE Congress 2023, Trebinje, Bosnia and Herzegovina 7-10th June 2023 (2023):56, https://hdl.handle.net/21.15107/rcub_technorep_7011 .