Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis
Abstract
Lipase from Candida rugosa was immobilized on Cuprophane membrane in a hollow-fibre module for possible application for oil hydrolysis. A simple immobilization technique was applied and 154.8 mg m(-2) lipase was immobilized with approximately 40% mass yield. The reactor operating regime was investigated with respect to flow patterns in reactor zones, lipase desorption and hydrolysis rates. Flow pattern studies in the experimental system revealed nearly plug flow with a stagnant region in the reactor lumen and significant flow channeling in the shell zone. Mass transfer limitations were apparent in the lumen zone at flow rates below 15 cm(3) min(-1). Higher flow rates up to 50 cm(3) min(-1) in the lumen zone and in the range 2.5-26 cm(3) min(-1) in the shell side had no apparent influence on hydrolysis rates. At the enzyme load employed in this study, loss of enzyme activity due to desorption had little effect on reactor stability and oil hydrolysis.
Keywords:
Candida rugosa / immobilized lipase / Cuprophane hollow fibre / palm oil hydrolysis / membrane reactor / reactor operating regimeSource:
Process Biochemistry, 2004, 39, 11, 1377-1385Publisher:
- Elsevier Sci Ltd, Oxford
DOI: 10.1016/S0032-9592(03)00268-1
ISSN: 1359-5113
WoS: 000223089500007
Scopus: 2-s2.0-3042810388
Institution/Community
Tehnološko-metalurški fakultetTY - JOUR AU - Knežević, Zorica AU - Kukić, Gojko AU - Vuković, Marija AU - Bugarski, Branko AU - Obradović, Bojana PY - 2004 UR - http://TechnoRep.tmf.bg.ac.rs/handle/123456789/704 AB - Lipase from Candida rugosa was immobilized on Cuprophane membrane in a hollow-fibre module for possible application for oil hydrolysis. A simple immobilization technique was applied and 154.8 mg m(-2) lipase was immobilized with approximately 40% mass yield. The reactor operating regime was investigated with respect to flow patterns in reactor zones, lipase desorption and hydrolysis rates. Flow pattern studies in the experimental system revealed nearly plug flow with a stagnant region in the reactor lumen and significant flow channeling in the shell zone. Mass transfer limitations were apparent in the lumen zone at flow rates below 15 cm(3) min(-1). Higher flow rates up to 50 cm(3) min(-1) in the lumen zone and in the range 2.5-26 cm(3) min(-1) in the shell side had no apparent influence on hydrolysis rates. At the enzyme load employed in this study, loss of enzyme activity due to desorption had little effect on reactor stability and oil hydrolysis. PB - Elsevier Sci Ltd, Oxford T2 - Process Biochemistry T1 - Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis EP - 1385 IS - 11 SP - 1377 VL - 39 DO - 10.1016/S0032-9592(03)00268-1 ER -
@article{ author = "Knežević, Zorica and Kukić, Gojko and Vuković, Marija and Bugarski, Branko and Obradović, Bojana", year = "2004", abstract = "Lipase from Candida rugosa was immobilized on Cuprophane membrane in a hollow-fibre module for possible application for oil hydrolysis. A simple immobilization technique was applied and 154.8 mg m(-2) lipase was immobilized with approximately 40% mass yield. The reactor operating regime was investigated with respect to flow patterns in reactor zones, lipase desorption and hydrolysis rates. Flow pattern studies in the experimental system revealed nearly plug flow with a stagnant region in the reactor lumen and significant flow channeling in the shell zone. Mass transfer limitations were apparent in the lumen zone at flow rates below 15 cm(3) min(-1). Higher flow rates up to 50 cm(3) min(-1) in the lumen zone and in the range 2.5-26 cm(3) min(-1) in the shell side had no apparent influence on hydrolysis rates. At the enzyme load employed in this study, loss of enzyme activity due to desorption had little effect on reactor stability and oil hydrolysis.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Process Biochemistry", title = "Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis", pages = "1385-1377", number = "11", volume = "39", doi = "10.1016/S0032-9592(03)00268-1" }
Knežević, Z., Kukić, G., Vuković, M., Bugarski, B.,& Obradović, B.. (2004). Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis. in Process Biochemistry Elsevier Sci Ltd, Oxford., 39(11), 1377-1385. https://doi.org/10.1016/S0032-9592(03)00268-1
Knežević Z, Kukić G, Vuković M, Bugarski B, Obradović B. Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis. in Process Biochemistry. 2004;39(11):1377-1385. doi:10.1016/S0032-9592(03)00268-1 .
Knežević, Zorica, Kukić, Gojko, Vuković, Marija, Bugarski, Branko, Obradović, Bojana, "Operating regime of a biphasic oil/aqueous hollow-fibre reactor with immobilized lipase for oil hydrolysis" in Process Biochemistry, 39, no. 11 (2004):1377-1385, https://doi.org/10.1016/S0032-9592(03)00268-1 . .